Rorug02G0043600

Thermospermine synthase ACAULIS5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0004438
Physical Location & Seq
Reverse (-)
3410046 .. 3410855
810 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0043600.1

Sequence Viewer

Length: 810 bp
ATGGGGAGCTACATTGTCTCTTGTGTCCACATAGTATTGAAGACACCTCTCACTTATTCTGCAAATGTCGATCCCTTGGCATCGACGATCCTCTCTGCTCCACCTTTTAACCTACAGTCGGAACTGTCTTTAGCGGCCAGCTTCAAAGATTGGATGCTAGACAGGGCCCTTACGTTGAGTGGTGAGGTTTTTGAGAAACTTATGATGATCTTGTGGGCTATTTGGAAAAACAGGAATAACTTCTTGTGGAATGGTACACAACAAACTGCTCAAGATATCCTTTTAAGCAGCTTTGCTTGGTTACATGAATTTCAGAAAGCGCAGGTCCCAGCTACACAGAAAGTGGCACAACGAAGACAGTTTTGGAACCCACCTGCGTCTGGAATCCTTAAATTGAATGTGGATGCCTCACACCTACCGAACCAAACTCGAGGGGGAGTTGGGGGTGTTTTGCGCAATAGCACTGGCCAGGTTATAGCAGCATTTGCTGGCCCCATCCCTCATGCCGCCTCCCCAAAGCAGAGTGAACTATATGCCATAAGAGCTGGCCTTGATCTGGTGAACACTCTAAGGAAGCAAGCTGTTATTATCGAAAGTGATTGTACTGAGGCAATTACAGAAGTTTCGTGTGTAGATCATAGTCTCCTTGGCAACGAAGGGCTAATTGATGATATCAAACATGCTATGGCTTCGATCTGTCAAGTGCAGCTGTGTCATACCCCTCGATCATGCAACCTAGTTGGCCATAGACTAGCGGGAATAGGCTTCGAAGCCACCCACCATGTGGTGTGGTTACAGGCACGGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spermine_synt_N Spermine_synth

Protein Analysis

269

Amino Acids

29.52

Weight (kDa)

7.71

Isoelectric Point (pI)

42.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 133 - 252 9.1e-18 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 382
Acc16I TGCGCA 1 cut(s) 455
Acc36I ACCTGC 2 cut(s) 313, 382
AccB7I CCANNNNNTGG 1 cut(s) 784
AciI CCGC 3 cut(s) 134, 507, 755
AclWI GGATC 2 cut(s) 65, 82
AcoI YGGCCR 3 cut(s) 135, 466, 742
AcsI RAATTY 1 cut(s) 308
AdeI CACNNNGTG 1 cut(s) 784
AfaI GTAC 2 cut(s) 256, 604
AfiI CCNNNNNNNGG 4 cut(s) 118, 380, 556, 784
AgsI TTSAA 3 cut(s) 40, 145, 397
AjnI CCWGG 1 cut(s) 468
AjuI GAANNNNNNNTTGG 2 cut(s) 346, 378
AluBI AGCT 7 cut(s) 9, 141, 291, 332, 545, 581, 709
AluI AGCT 7 cut(s) 9, 141, 291, 332, 545, 581, 709
Alw26I GTCTC 2 cut(s) 22, 647
AlwI GGATC 2 cut(s) 65, 82
Ama87I CYCGRG 1 cut(s) 429
AoxI GGCC 6 cut(s) 135, 165, 466, 490, 547, 742
ApaI GGGCCC 1 cut(s) 169
ApeKI GCWGC 3 cut(s) 288, 479, 706
ApoI RAATTY 1 cut(s) 308
Asp700I GAANNNNTTC 1 cut(s) 239
AspLEI GCGC 2 cut(s) 322, 456
AspS9I GGNCC 4 cut(s) 165, 166, 325, 491
AsuHPI GGTGA 2 cut(s) 194, 571
AsuII TTCGAA 1 cut(s) 768
AvaI CYCGRG 1 cut(s) 429
AvaII GGWCC 1 cut(s) 325
BaeGI GKGCMC 1 cut(s) 169
BalI TGGCCA 2 cut(s) 468, 744
BanII GRGCYC 1 cut(s) 169
BbsI GAAGAC 2 cut(s) 47, 361
BbvI GCAGC 3 cut(s) 300, 491, 718
BccI CCATC 1 cut(s) 503
BciT130I CCWGG 1 cut(s) 470
BcoDI GTCTC 2 cut(s) 22, 647
BfaI CTAG 3 cut(s) 158, 737, 752
BfmI CTRYAG 1 cut(s) 113
BfuAI ACCTGC 2 cut(s) 313, 382
BisI GCNGC 5 cut(s) 135, 289, 480, 507, 707
BlsI GCNGC 5 cut(s) 136, 290, 481, 508, 708
Bme1390I CCNGG 1 cut(s) 470
Bme18I GGWCC 1 cut(s) 325
BmeT110I CYCGRG 1 cut(s) 429
BmgT120I GGNCC 4 cut(s) 165, 166, 325, 491
BmiI GGNNCC 4 cut(s) 167, 327, 368, 493
BmrFI CCNGG 1 cut(s) 470
BmsI GCATC 3 cut(s) 89, 144, 394
BpiI GAAGAC 2 cut(s) 47, 361
Bpu14I TTCGAA 1 cut(s) 768
BpuEI CTTGAG 1 cut(s) 255
BsaJI CCNNGG 2 cut(s) 75, 646
Bsc4I CCNNNNNNNGG 4 cut(s) 118, 380, 556, 784
Bse1I ACTGG 1 cut(s) 469
BseBI CCWGG 1 cut(s) 470
BseDI CCNNGG 2 cut(s) 75, 646
BseGI GGATG 4 cut(s) 159, 409, 495, 810
BseLI CCNNNNNNNGG 4 cut(s) 118, 380, 556, 784
BseMII CTCAG 1 cut(s) 597
BseNI ACTGG 1 cut(s) 469
BseSI GKGCMC 1 cut(s) 169
BseXI GCAGC 3 cut(s) 300, 491, 718
BseYI CCCAGC 1 cut(s) 328
BsgI GTGCAG 1 cut(s) 725
BshFI GGCC 6 cut(s) 137, 167, 468, 492, 549, 744
BsiHKCI CYCGRG 1 cut(s) 429
BslFI GGGAC 1 cut(s) 311
BslI CCNNNNNNNGG 4 cut(s) 118, 380, 556, 784
BsmAI GTCTC 2 cut(s) 22, 647
BsmFI GGGAC 1 cut(s) 311
BsnI GGCC 6 cut(s) 137, 167, 468, 492, 549, 744
BsoBI CYCGRG 1 cut(s) 429
Bsp119I TTCGAA 1 cut(s) 768
Bsp120I GGGCCC 1 cut(s) 165
Bsp1286I GDGCHC 1 cut(s) 169
Bsp143I GATC 7 cut(s) 70, 87, 207, 553, 634, 693, 725
BspACI CCGC 3 cut(s) 134, 507, 755
BspANI GGCC 6 cut(s) 137, 167, 468, 492, 549, 744
BspCNI CTCAG 1 cut(s) 598
BspLI GGNNCC 4 cut(s) 167, 327, 368, 493
BspMI ACCTGC 2 cut(s) 313, 382
BspPI GGATC 2 cut(s) 65, 82
BspT104I TTCGAA 1 cut(s) 768
BsrI ACTGG 1 cut(s) 469
BssECI CCNNGG 2 cut(s) 75, 646
BssMI GATC 7 cut(s) 70, 87, 207, 553, 634, 693, 725
BssT1I CCWWGG 2 cut(s) 75, 646
Bst2UI CCWGG 1 cut(s) 470
Bst4CI ACNGT 3 cut(s) 117, 126, 360
BstAPI GCANNNNNTGC 1 cut(s) 485
BstBI TTCGAA 1 cut(s) 768
BstC8I GCNNGC 4 cut(s) 139, 490, 547, 579
BstDEI CTNAG 2 cut(s) 569, 606
BstF5I GGATG 4 cut(s) 159, 409, 495, 810
BstHHI GCGC 2 cut(s) 322, 456
BstKTI GATC 7 cut(s) 73, 90, 210, 556, 637, 696, 728
BstMAI GTCTC 2 cut(s) 22, 647
BstMBI GATC 7 cut(s) 70, 87, 207, 553, 634, 693, 725
BstMWI GCNNNNNNNGC 2 cut(s) 485, 542
BstNI CCWGG 1 cut(s) 470
BstNSI RCATGY 1 cut(s) 683
BstSCI CCNGG 1 cut(s) 468
BstSFI CTRYAG 1 cut(s) 113
BstSLI GKGCMC 1 cut(s) 169
BstV1I GCAGC 3 cut(s) 300, 491, 718
BstV2I GAAGAC 2 cut(s) 47, 361
BsuRI GGCC 6 cut(s) 137, 167, 468, 492, 549, 744
BtsCI GGATG 4 cut(s) 159, 409, 495, 810
BtsIMutI CAGTG 1 cut(s) 462
BveI ACCTGC 2 cut(s) 313, 382
Cac8I GCNNGC 4 cut(s) 139, 490, 547, 579
CfoI GCGC 2 cut(s) 322, 456
Cfr13I GGNCC 4 cut(s) 165, 166, 325, 491
CseI GACGC 1 cut(s) 366
Csp6I GTAC 2 cut(s) 255, 603
CviAII CATG 5 cut(s) 305, 503, 680, 729, 782
CviQI GTAC 2 cut(s) 255, 603
DdeI CTNAG 2 cut(s) 569, 606
DpnI GATC 7 cut(s) 72, 89, 209, 555, 636, 695, 727
DpnII GATC 7 cut(s) 70, 87, 207, 553, 634, 693, 725
DraIII CACNNNGTG 1 cut(s) 784
EaeI YGGCCR 3 cut(s) 135, 466, 742
Eco130I CCWWGG 2 cut(s) 75, 646
Eco24I GRGCYC 1 cut(s) 169
Eco32I GATATC 2 cut(s) 277, 673
Eco47I GGWCC 1 cut(s) 325
Eco88I CYCGRG 1 cut(s) 429
EcoO109I RGGNCCY 3 cut(s) 165, 166, 325
EcoRII CCWGG 1 cut(s) 468
EcoRV GATATC 2 cut(s) 277, 673
EcoT14I CCWWGG 2 cut(s) 75, 646
EcoT38I GRGCYC 1 cut(s) 169
ErhI CCWWGG 2 cut(s) 75, 646
FaeI CATG 5 cut(s) 308, 506, 683, 732, 785
FalI AAGNNNNNCTT 2 cut(s) 264, 296
FaqI GGGAC 1 cut(s) 311
FatI CATG 5 cut(s) 304, 502, 679, 728, 781
FauI CCCGC 1 cut(s) 748
Fnu4HI GCNGC 5 cut(s) 135, 289, 480, 507, 707
FokI GGATG 3 cut(s) 166, 416, 482
FriOI GRGCYC 1 cut(s) 169
Fsp4HI GCNGC 5 cut(s) 135, 289, 480, 507, 707
FspBI CTAG 3 cut(s) 158, 737, 752
FspI TGCGCA 1 cut(s) 455
GlaI GCGC 2 cut(s) 321, 455
GluI GCNGC 5 cut(s) 135, 289, 480, 507, 707
GsaI CCCAGC 1 cut(s) 332
HaeIII GGCC 6 cut(s) 137, 167, 468, 492, 549, 744
HgaI GACGC 1 cut(s) 366
HhaI GCGC 2 cut(s) 322, 456
Hin1II CATG 5 cut(s) 308, 506, 683, 732, 785
Hin6I GCGC 2 cut(s) 320, 454
HinP1I GCGC 2 cut(s) 320, 454
HinfI GANTC 1 cut(s) 384
HphI GGTGA 2 cut(s) 194, 571
Hpy166II GTNNAC 4 cut(s) 28, 257, 527, 562
Hpy188I TCNGA 2 cut(s) 121, 315
Hpy188III TCNNGA 2 cut(s) 272, 381
Hpy8I GTNNAC 4 cut(s) 28, 257, 527, 562
Hpy99I CGWCG 1 cut(s) 88
HpyAV CCTTC 1 cut(s) 650
HpyCH4III ACNGT 3 cut(s) 117, 126, 360
HpyCH4IV ACGT 1 cut(s) 173
HpyCH4V TGCA 3 cut(s) 62, 706, 732
HpyF10VI GCNNNNNNNGC 2 cut(s) 485, 542
HpyF3I CTNAG 2 cut(s) 569, 606
HpySE526I ACGT 1 cut(s) 173
Hsp92II CATG 5 cut(s) 308, 506, 683, 732, 785
HspAI GCGC 2 cut(s) 320, 454
Kzo9I GATC 7 cut(s) 70, 87, 207, 553, 634, 693, 725
LmnI GCTCC 2 cut(s) 6, 103
Lsp1109I GCAGC 3 cut(s) 300, 491, 718
LweI GCATC 3 cut(s) 89, 144, 394
MaeI CTAG 3 cut(s) 158, 737, 752
MaeII ACGT 1 cut(s) 173
MaeIII GTNAC 2 cut(s) 300, 792
MalI GATC 7 cut(s) 72, 89, 209, 555, 636, 695, 727
MboI GATC 7 cut(s) 70, 87, 207, 553, 634, 693, 725
MboII GAAGA 2 cut(s) 52, 366
MhlI GDGCHC 1 cut(s) 169
MlsI TGGCCA 2 cut(s) 468, 744
MluCI AATT 4 cut(s) 308, 392, 612, 663
MluNI TGGCCA 2 cut(s) 468, 744
MmeI TCCRAC 1 cut(s) 99
MnlI CCTC 9 cut(s) 57, 101, 178, 418, 425, 510, 520, 601, 732
Mox20I TGGCCA 2 cut(s) 468, 744
MroXI GAANNNNTTC 1 cut(s) 239
MscI TGGCCA 2 cut(s) 468, 744
MseI TTAA 3 cut(s) 108, 284, 390
Msp20I TGGCCA 2 cut(s) 468, 744
MspA1I CMGCKG 1 cut(s) 709
MspR9I CCNGG 1 cut(s) 470
MvaI CCWGG 1 cut(s) 470
MwoI GCNNNNNNNGC 2 cut(s) 485, 542
NdeII GATC 7 cut(s) 70, 87, 207, 553, 634, 693, 725
NlaIII CATG 5 cut(s) 308, 506, 683, 732, 785
NlaIV GGNNCC 4 cut(s) 167, 327, 368, 493
NsbI TGCGCA 1 cut(s) 455
NspI RCATGY 1 cut(s) 683
NspV TTCGAA 1 cut(s) 768
PaeR7I CTCGAG 1 cut(s) 429
PaqCI CACCTGC 1 cut(s) 382
PdmI GAANNNNTTC 1 cut(s) 239
PfeI GAWTC 1 cut(s) 384
PflMI CCANNNNNTGG 1 cut(s) 784
PkrI GCNGC 5 cut(s) 136, 290, 481, 508, 708
PpuMI RGGWCCY 1 cut(s) 325
Psp5II RGGWCCY 1 cut(s) 325
Psp6I CCWGG 1 cut(s) 468
PspFI CCCAGC 1 cut(s) 328
PspGI CCWGG 1 cut(s) 468
PspN4I GGNNCC 4 cut(s) 167, 327, 368, 493
PspOMI GGGCCC 1 cut(s) 165
PspPI GGNCC 4 cut(s) 165, 166, 325, 491
PspPPI RGGWCCY 1 cut(s) 325
PspXI VCTCGAGB 1 cut(s) 429
PvuII CAGCTG 1 cut(s) 709
RsaI GTAC 2 cut(s) 256, 604
RsaNI GTAC 2 cut(s) 255, 603
SaqAI TTAA 3 cut(s) 108, 284, 390
SatI GCNGC 5 cut(s) 135, 289, 480, 507, 707
Sau3AI GATC 7 cut(s) 70, 87, 207, 553, 634, 693, 725
Sau96I GGNCC 4 cut(s) 165, 166, 325, 491
ScrFI CCNGG 1 cut(s) 470
SduI GDGCHC 1 cut(s) 169
SfaNI GCATC 3 cut(s) 89, 144, 394
SfcI CTRYAG 1 cut(s) 113
Sfr274I CTCGAG 1 cut(s) 429
SfuI TTCGAA 1 cut(s) 768
SinI GGWCC 1 cut(s) 325
SlaI CTCGAG 1 cut(s) 429
SmlI CTYRAG 2 cut(s) 270, 429
SmoI CTYRAG 2 cut(s) 270, 429
Sse9I AATT 4 cut(s) 308, 392, 612, 663
SsiI CCGC 3 cut(s) 134, 507, 755
SspMI CTAG 3 cut(s) 158, 737, 752
StyD4I CCNGG 1 cut(s) 468
StyI CCWWGG 2 cut(s) 75, 646
TaaI ACNGT 3 cut(s) 117, 126, 360
TaiI ACGT 1 cut(s) 176
TaqI TCGA 7 cut(s) 69, 83, 430, 591, 692, 724, 768
TasI AATT 4 cut(s) 308, 392, 612, 663
TatI WGTACW 1 cut(s) 602
TauI GCSGC 2 cut(s) 137, 509
TfiI GAWTC 1 cut(s) 384
Tru1I TTAA 3 cut(s) 108, 284, 390
Tru9I TTAA 3 cut(s) 108, 284, 390
TscAI CASTG 1 cut(s) 469
TseI GCWGC 3 cut(s) 288, 479, 706
TspDTI ATGAA 1 cut(s) 321
TspRI CASTG 1 cut(s) 469
Van91I CCANNNNNTGG 1 cut(s) 784
VpaK11BI GGWCC 1 cut(s) 325
XapI RAATTY 1 cut(s) 308
XceI RCATGY 1 cut(s) 683
XcmI CCANNNNNNNNNTGG 1 cut(s) 781
XhoI CTCGAG 1 cut(s) 429
XmnI GAANNNNTTC 1 cut(s) 239
XspI CTAG 3 cut(s) 158, 737, 752
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.