Rh2BG091400

Thermospermine synthase ACAULIS5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0004438
Physical Location & Seq
Reverse (-)
7131397 .. 7132882
1486 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG091400.1

Sequence Viewer

Length: 576 bp
ATGGGAGGTGGTGAAGGGTCAACAGCAAGGGAGGCACTGAAGCACAAAGATGTGGAGAAAGTTATCATGTGTGATATAGATGAGGTGATTGTCAATTTTTGCCGTGCACATCTGGAAGAAAATCAAATAGGCTTCAACTTGTTTTCAATGATGCTAATCATAAAACCTCACCTCAAAGACAATGGCATCTTTGTCACTCAAGCTGGTCCAGCAGGAATTCTTTCTCATAAGGCTATCTTCACTTCAATATACAACACTGTAAAACAAGTGTTCAAATATGTGTTGGCACACACAGCTCATTTGCCATCTTATGCAGATTCATGTGGATGGGTGATGGCTTCAGATCAACCACTCAGATTGGATGCTGCACAACTTGATAGCAGGATTGAGGAACAAATCCGAGGCAAGCTACTTTATTTAGATGGTGCTTTCATTGTCTCATCAACTGTGGTCAACAAGACGATCTACACATCGCTTTTGAAAGGAACTAATGTCTTCACTGAAGAAACTACAAAGTTCTGCCAGGGACATGGAGTCACTGATGATGCACAAGGCAATGAGGTGATTGAGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spermine_synt_N Spermine_synth

Protein Analysis

191

Amino Acids

21.05

Weight (kDa)

5.6

Isoelectric Point (pI)

25.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spermine_synth PF01564 2 - 46 2.7e-10 Spermine/spermidine synthase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 216
AcuI CTGAAG 3 cut(s) 59, 324, 522
AgsI TTSAA 5 cut(s) 136, 147, 246, 274, 481
AhdI GACNNNNNGTC 1 cut(s) 533
AjnI CCWGG 1 cut(s) 522
AluBI AGCT 3 cut(s) 203, 296, 409
AluI AGCT 3 cut(s) 203, 296, 409
Alw21I GWGCWC 1 cut(s) 109
Alw26I GTCTC 1 cut(s) 442
Alw44I GTGCAC 1 cut(s) 105
ApaLI GTGCAC 1 cut(s) 105
ApeKI GCWGC 1 cut(s) 365
ApoI RAATTY 1 cut(s) 216
Asp700I GAANNNNTTC 1 cut(s) 220
AspS9I GGNCC 1 cut(s) 206
AsuHPI GGTGA 5 cut(s) 23, 97, 161, 343, 574
AvaII GGWCC 1 cut(s) 206
BaeGI GKGCMC 1 cut(s) 109
BbsI GAAGAC 1 cut(s) 487
Bbv12I GWGCWC 1 cut(s) 109
BbvI GCAGC 1 cut(s) 352
BccI CCATC 4 cut(s) 313, 321, 328, 416
BceAI ACGGC 1 cut(s) 87
BciT130I CCWGG 1 cut(s) 524
BcoDI GTCTC 1 cut(s) 442
BisI GCNGC 1 cut(s) 366
BlsI GCNGC 1 cut(s) 367
Bme1390I CCNGG 1 cut(s) 524
Bme18I GGWCC 1 cut(s) 206
BmeRI GACNNNNNGTC 1 cut(s) 533
BmgT120I GGNCC 1 cut(s) 206
BmrFI CCNGG 1 cut(s) 524
BmsI GCATC 4 cut(s) 141, 195, 352, 535
BpiI GAAGAC 1 cut(s) 487
BpuEI CTTGAG 1 cut(s) 183
BsaBI GATNNNNATC 1 cut(s) 155
BsaJI CCNNGG 2 cut(s) 400, 523
Bse3DI GCAATG 1 cut(s) 562
Bse8I GATNNNNATC 1 cut(s) 155
BseBI CCWGG 1 cut(s) 524
BseDI CCNNGG 2 cut(s) 400, 523
BseGI GGATG 2 cut(s) 332, 367
BseJI GATNNNNATC 1 cut(s) 155
BseMI GCAATG 1 cut(s) 562
BseMII CTCAG 1 cut(s) 367
BseSI GKGCMC 1 cut(s) 109
BseXI GCAGC 1 cut(s) 352
BsgI GTGCAG 1 cut(s) 351
BsiHKAI GWGCWC 1 cut(s) 109
BslFI GGGAC 1 cut(s) 540
BsmAI GTCTC 1 cut(s) 442
BsmFI GGGAC 1 cut(s) 540
Bsp1286I GDGCHC 1 cut(s) 109
Bsp143I GATC 2 cut(s) 343, 462
BspCNI CTCAG 1 cut(s) 366
BsrDI GCAATG 1 cut(s) 562
BssECI CCNNGG 2 cut(s) 400, 523
BssMI GATC 2 cut(s) 343, 462
Bst2UI CCWGG 1 cut(s) 524
Bst4CI ACNGT 2 cut(s) 259, 448
BstC8I GCNNGC 1 cut(s) 407
BstDEI CTNAG 1 cut(s) 353
BstF5I GGATG 2 cut(s) 332, 367
BstKTI GATC 2 cut(s) 346, 465
BstMAI GTCTC 1 cut(s) 442
BstMBI GATC 2 cut(s) 343, 462
BstMWI GCNNNNNNNGC 3 cut(s) 32, 209, 293
BstNI CCWGG 1 cut(s) 524
BstSCI CCNGG 1 cut(s) 522
BstSLI GKGCMC 1 cut(s) 109
BstV1I GCAGC 1 cut(s) 352
BstV2I GAAGAC 1 cut(s) 487
BstXI CCANNNNNNTGG 1 cut(s) 530
BtgZI GCGATG 1 cut(s) 456
BtsCI GGATG 2 cut(s) 332, 367
BtsIMutI CAGTG 4 cut(s) 35, 255, 498, 537
Cac8I GCNNGC 1 cut(s) 407
Cfr13I GGNCC 1 cut(s) 206
CspCI CAANNNNNGTGG 2 cut(s) 339, 374
CviAII CATG 3 cut(s) 67, 321, 530
CviJI RGCY 6 cut(s) 132, 203, 233, 296, 338, 409
CviKI_1 RGCY 6 cut(s) 132, 203, 233, 296, 338, 409
DdeI CTNAG 1 cut(s) 353
DpnI GATC 2 cut(s) 345, 464
DpnII GATC 2 cut(s) 343, 462
DriI GACNNNNNGTC 1 cut(s) 533
Eam1105I GACNNNNNGTC 1 cut(s) 533
Eco47I GGWCC 1 cut(s) 206
Eco57I CTGAAG 3 cut(s) 59, 324, 522
EcoRI GAATTC 1 cut(s) 216
EcoRII CCWGG 1 cut(s) 522
FaeI CATG 3 cut(s) 70, 324, 533
FaiI YATR 9 cut(s) 68, 77, 161, 228, 250, 279, 312, 322, 531
FalI AAGNNNNNCTT 2 cut(s) 221, 253
FaqI GGGAC 1 cut(s) 540
FatI CATG 3 cut(s) 66, 320, 529
Fnu4HI GCNGC 1 cut(s) 366
FokI GGATG 2 cut(s) 339, 374
Fsp4HI GCNGC 1 cut(s) 366
GluI GCNGC 1 cut(s) 366
Hin1II CATG 3 cut(s) 70, 324, 533
HincII GTYRAC 2 cut(s) 21, 454
HindII GTYRAC 2 cut(s) 21, 454
HinfI GANTC 2 cut(s) 317, 534
HphI GGTGA 5 cut(s) 23, 97, 161, 343, 574
Hpy166II GTNNAC 3 cut(s) 21, 107, 454
Hpy188I TCNGA 3 cut(s) 343, 356, 401
Hpy188III TCNNGA 1 cut(s) 113
Hpy8I GTNNAC 3 cut(s) 21, 107, 454
HpyAV CCTTC 1 cut(s) 8
HpyCH4III ACNGT 2 cut(s) 259, 448
HpyCH4V TGCA 4 cut(s) 107, 314, 368, 548
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 209, 293
HpyF3I CTNAG 1 cut(s) 353
Hsp92II CATG 3 cut(s) 70, 324, 533
Kzo9I GATC 2 cut(s) 343, 462
LpnPI CCDG 7 cut(s) 98, 189, 198, 222, 367, 509, 536
Lsp1109I GCAGC 1 cut(s) 352
LweI GCATC 4 cut(s) 141, 195, 352, 535
MaeIII GTNAC 2 cut(s) 193, 535
MalI GATC 2 cut(s) 345, 464
MboI GATC 2 cut(s) 343, 462
MboII GAAGA 4 cut(s) 128, 229, 487, 515
MhlI GDGCHC 1 cut(s) 109
MluCI AATT 2 cut(s) 94, 216
MlyI GAGTC 1 cut(s) 543
MnlI CCTC 8 cut(s) 25, 76, 177, 182, 382, 395, 553, 562
MroXI GAANNNNTTC 1 cut(s) 220
MslI CAYNNNNRTG 2 cut(s) 48, 325
MspR9I CCNGG 1 cut(s) 524
MvaI CCWGG 1 cut(s) 524
MwoI GCNNNNNNNGC 3 cut(s) 32, 209, 293
NdeII GATC 2 cut(s) 343, 462
NlaIII CATG 3 cut(s) 70, 324, 533
NmuCI GTSAC 2 cut(s) 193, 535
PdmI GAANNNNTTC 1 cut(s) 220
PfeI GAWTC 1 cut(s) 317
PkrI GCNGC 1 cut(s) 367
PleI GAGTC 1 cut(s) 542
PpsI GAGTC 1 cut(s) 542
Psp6I CCWGG 1 cut(s) 522
PspGI CCWGG 1 cut(s) 522
PspPI GGNCC 1 cut(s) 206
RseI CAYNNNNRTG 2 cut(s) 48, 325
SatI GCNGC 1 cut(s) 366
Sau3AI GATC 2 cut(s) 343, 462
Sau96I GGNCC 1 cut(s) 206
SchI GAGTC 1 cut(s) 543
ScrFI CCNGG 1 cut(s) 524
SduI GDGCHC 1 cut(s) 109
SetI ASST 9 cut(s) 10, 87, 169, 174, 205, 298, 411, 564, 573
SfaNI GCATC 4 cut(s) 141, 195, 352, 535
SinI GGWCC 1 cut(s) 206
SmiMI CAYNNNNRTG 2 cut(s) 48, 325
SmlI CTYRAG 1 cut(s) 198
SmoI CTYRAG 1 cut(s) 198
Sse9I AATT 2 cut(s) 94, 216
StyD4I CCNGG 1 cut(s) 522
TaaI ACNGT 2 cut(s) 259, 448
TasI AATT 2 cut(s) 94, 216
TfiI GAWTC 1 cut(s) 317
TscAI CASTG 4 cut(s) 42, 262, 505, 544
TseFI GTSAC 2 cut(s) 193, 535
TseI GCWGC 1 cut(s) 365
Tsp45I GTSAC 2 cut(s) 193, 535
TspDTI ATGAA 2 cut(s) 309, 421
TspRI CASTG 4 cut(s) 42, 262, 505, 544
VneI GTGCAC 1 cut(s) 105
VpaK11BI GGWCC 1 cut(s) 206
XapI RAATTY 1 cut(s) 216
XmnI GAANNNNTTC 1 cut(s) 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.