MD08G1095600.v1.1

Long chain base biosynthesis protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0004129
Physical Location & Seq
Forward (+)
8005996 .. 8011954
5959 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1095600.v1.1.491

Sequence Viewer

Length: 1461 bp
ATGTCATCAAGTATTCTGAACATAGTGAATGCTACGGTAGATTGGGTGACGGCGACTTTAGATGCTCCCTCTTCTCGAGCTGTTATTTTCGGATTTCCAATTGGCGGACATTTATTTGTAGAAGTTCTGCTTTTCTTAGTCATCGTTTATTTATTTTCCCAGAACAGTTACAGACCACCTAGGCGGCCTTTAACAGAGCAGGAAAAAGATGAGCTATGTGAGGAATGGGCTCCTGAACCTCTTATTCCTTCTATCACTGAAGAGAAGCTGTATGAACCCCCGGTGTTGGAAAGTGCTGCAGGGCCACATACAATAATCAAGGGTAAAGAAGTCGTGAACTTTGCTTCAGCAAATTATCTGGGATTGATAGGACATGAGAAGTTACTTGAGTCATGTACCTCTGCATTGGAAAAATATGGTGTTGGTTCATGTGGTCCTCGTGGGTTTTATGGAACAATTGATGTCCACCTTGATTGTGAGGCGAGAATAGCAAACTTCTTGGGAACTCCGGACTCAATTCTATATTCTTATGGACTGTCTACCATGTTCAGTGCAATTCCAGCATTTGCCAAGAAGGGGGATATTATTGTTGTAGATGAAGGTGTCCATTGGGGAATACAAAATGGTCTTCATCTTTCTAGGAGCACAATCAAGTATTTCAAGCACAATGACATGGAAGATTTAAGGAAAATTCTGAAGGAACTCACTTCAAATAAGAAAAGTGAGCGAGTTAAGAAATTGCGGCGCTACATTGTAGTTGAAGCTGTATACCAGAATTCTGGCCAAATAGCCCCCTTGGACAAGATTGTTGAATTGAAGGAGAAATATAAGTTTCGTGTTTTATTGGATGAGAGTAACTCGTTTGGCGTGCTTGGAAAATCCGGAAGGGGTCTCACTGAATACTGTAGGATTCCGGTTGAAAAAATAGATATTATTACTGCTGCAATGGGACACGCATTAGCCACAGAAGGAGGATTCTGCACTGGAAGTGCTAGGGTCACTGATCACCAACGATTGAGCAGCTCTGGTTACGTCTTTTCTGCTTCTTTGCCCCCATATCTTGCGAGTGCTGCCATTACTGCTATTGATATCCTCGAGGAAAACCCTGACCTGATAACGAAGTTGAAGAAAAACATTGCCGAATTATGGAAGGGATTGTCAGGTATACGGGGCCTCTCATTCGCGAGCAGTCCAGAATCACCGATTGTCTTCCTCAGACTAGAGAAAGCAGACTCTTTGAAGAATGAACTGCAACTTCAGCTCCTTCAAAATATTGCTAACAGTTTGTTAAAGGAGCATTCCATTTTCGTGGTCACCTCCCAAAAGTCAACACTTGATAAATGCCGTCTTCCATTAGGAATTAGATTGTTCGTCTCCGCTGGGCATACAGAATCCGATCTGCTCAAGGCAGCTGCATCACTGAAAAGCGTTGCAGAATTGGTGCTGAAGGATCATATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0002178 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006066 GO:0006629 GO:0006643 GO:0006644 GO:0006665 GO:0006667 GO:0006670 GO:0006672 GO:0006684 GO:0006686 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009058 GO:0009790 GO:0009791 GO:0009793 GO:0009825 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010154 GO:0010506 GO:0010508 GO:0010941 GO:0012505 GO:0016020 GO:0016049 GO:0016239 GO:0016241 GO:0017059 GO:0019222 GO:0019637 GO:0019751 GO:0022414 GO:0030148 GO:0031211 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031984 GO:0032501 GO:0032502 GO:0032991 GO:0034311 GO:0034312 GO:0034641 GO:0035339 GO:0040007 GO:0042175 GO:0043067 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046165 GO:0046173 GO:0046467 GO:0046511 GO:0046512 GO:0046513 GO:0046519 GO:0046520 GO:0048316 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0061458 GO:0065007 GO:0071704 GO:0090407 GO:0097164 GO:0098796 GO:0098827 GO:1901564 GO:1901566 GO:1901576 GO:1901615 GO:1901617 GO:1902494 GO:1904502 GO:1904504 GO:1905961 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00600 ko01100 ko04071 ko04138 map00600 map01100 map04071 map04138
Pfam Domains
Protein Families
Aminotran_1_2

Protein Analysis

487

Amino Acids

53.51

Weight (kDa)

6.66

Isoelectric Point (pI)

36.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 110 - 474 7.2e-38 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 1094
AccI GTMKAC 3 cut(s) 539, 768, 1165
AccII CGCG 1 cut(s) 1184
AccIII TCCGGA 2 cut(s) 508, 881
AciI CCGC 4 cut(s) 105, 184, 742, 1377
AclWI GGATC 1 cut(s) 1458
AcoI YGGCCR 1 cut(s) 781
AcsI RAATTY 2 cut(s) 690, 775
AcuI CTGAAG 4 cut(s) 279, 330, 716, 1241
AfaI GTAC 1 cut(s) 397
AfiI CCNNNNNNNGG 4 cut(s) 104, 286, 576, 1146
AgsI TTSAA 9 cut(s) 661, 711, 761, 812, 817, 920, 1126, 1240, 1268
AluBI AGCT 7 cut(s) 80, 214, 268, 764, 1023, 1261, 1412
AluI AGCT 7 cut(s) 80, 214, 268, 764, 1023, 1261, 1412
Alw21I GWGCWC 1 cut(s) 647
Alw26I GTCTC 2 cut(s) 896, 1378
AlwI GGATC 1 cut(s) 1458
Ama87I CYCGRG 2 cut(s) 75, 1094
Aor13HI TCCGGA 2 cut(s) 508, 881
AoxI GGCC 4 cut(s) 185, 302, 781, 1171
ApeKI GCWGC 6 cut(s) 296, 941, 1020, 1070, 1409, 1412
ApoI RAATTY 2 cut(s) 690, 775
AspA2I CCTAGG 1 cut(s) 179
AspLEI GCGC 1 cut(s) 747
AspS9I GGNCC 3 cut(s) 302, 434, 1171
AsuC2I CCSGG 1 cut(s) 281
AsuHPI GGTGA 4 cut(s) 58, 998, 1191, 1306
AvaI CYCGRG 2 cut(s) 75, 1094
AvaII GGWCC 1 cut(s) 434
AvrII CCTAGG 1 cut(s) 179
BalI TGGCCA 1 cut(s) 783
BanII GRGCYC 1 cut(s) 232
BauI CACGAG 1 cut(s) 438
BbsI GAAGAC 3 cut(s) 620, 1201, 1340
Bbv12I GWGCWC 1 cut(s) 647
BbvI GCAGC 6 cut(s) 283, 928, 1032, 1057, 1399, 1421
BceAI ACGGC 2 cut(s) 66, 1329
BclI TGATCA 1 cut(s) 1003
BcnI CCSGG 1 cut(s) 281
BcoDI GTCTC 2 cut(s) 896, 1378
BfaI CTAG 4 cut(s) 180, 639, 993, 1220
BfmI CTRYAG 2 cut(s) 297, 904
BfoI RGCGCY 1 cut(s) 748
BisI GCNGC 8 cut(s) 185, 297, 743, 942, 1021, 1071, 1410, 1413
BlnI CCTAGG 1 cut(s) 179
BlsI GCNGC 8 cut(s) 186, 298, 744, 943, 1022, 1072, 1411, 1414
Bme1390I CCNGG 1 cut(s) 281
Bme18I GGWCC 1 cut(s) 434
BmeT110I CYCGRG 2 cut(s) 75, 1094
BmgT120I GGNCC 3 cut(s) 302, 434, 1171
BmiI GGNNCC 2 cut(s) 231, 1172
BmrFI CCNGG 1 cut(s) 281
BmsI GCATC 2 cut(s) 52, 1424
BpiI GAAGAC 3 cut(s) 620, 1201, 1340
BplI GAGNNNNNCTC 2 cut(s) 842, 874
BpuEI CTTGAG 2 cut(s) 407, 1388
BpuMI CCSGG 1 cut(s) 281
BsaI GGTCTC 1 cut(s) 896
BsaJI CCNNGG 3 cut(s) 179, 279, 795
BsaWI WCCGGW 3 cut(s) 508, 881, 913
BsaXI ACNNNNNCTCC 2 cut(s) 1245, 1275
Bsc4I CCNNNNNNNGG 4 cut(s) 104, 286, 576, 1146
Bse1I ACTGG 1 cut(s) 988
Bse3DI GCAATG 2 cut(s) 951, 1134
BseAI TCCGGA 2 cut(s) 508, 881
BseDI CCNNGG 3 cut(s) 179, 279, 795
BseGI GGATG 1 cut(s) 853
BseLI CCNNNNNNNGG 4 cut(s) 104, 286, 576, 1146
BseMI GCAATG 2 cut(s) 951, 1134
BseMII CTCAG 1 cut(s) 1228
BseNI ACTGG 1 cut(s) 988
BseXI GCAGC 6 cut(s) 283, 928, 1032, 1057, 1399, 1421
BseYI CCCAGC 1 cut(s) 1379
BsgI GTGCAG 1 cut(s) 964
Bsh1236I CGCG 1 cut(s) 1184
BshFI GGCC 4 cut(s) 187, 304, 783, 1173
BsiHKAI GWGCWC 1 cut(s) 647
BsiHKCI CYCGRG 2 cut(s) 75, 1094
BsiSI CCGG 4 cut(s) 281, 509, 882, 914
BslFI GGGAC 1 cut(s) 963
BslI CCNNNNNNNGG 4 cut(s) 104, 286, 576, 1146
BsmAI GTCTC 2 cut(s) 896, 1378
BsmBI CGTCTC 1 cut(s) 1378
BsmFI GGGAC 1 cut(s) 963
BsmI GAATGC 2 cut(s) 34, 1297
BsnI GGCC 4 cut(s) 187, 304, 783, 1173
Bso31I GGTCTC 1 cut(s) 896
BsoBI CYCGRG 2 cut(s) 75, 1094
Bsp1286I GDGCHC 2 cut(s) 232, 647
Bsp13I TCCGGA 2 cut(s) 508, 881
Bsp143I GATC 3 cut(s) 1003, 1396, 1450
Bsp68I TCGCGA 1 cut(s) 1184
BspACI CCGC 4 cut(s) 105, 184, 742, 1377
BspANI GGCC 4 cut(s) 187, 304, 783, 1173
BspCNI CTCAG 1 cut(s) 1227
BspEI TCCGGA 2 cut(s) 508, 881
BspFNI CGCG 1 cut(s) 1184
BspLI GGNNCC 2 cut(s) 231, 1172
BspMAI CTGCAG 1 cut(s) 301
BspPI GGATC 1 cut(s) 1458
BspTNI GGTCTC 1 cut(s) 896
BsrDI GCAATG 2 cut(s) 951, 1134
BsrI ACTGG 1 cut(s) 988
BssECI CCNNGG 3 cut(s) 179, 279, 795
BssMI GATC 3 cut(s) 1003, 1396, 1450
BssNAI GTATAC 2 cut(s) 769, 1166
BssSI CACGAG 1 cut(s) 438
BssT1I CCWWGG 2 cut(s) 179, 795
Bst1107I GTATAC 2 cut(s) 769, 1166
Bst2BI CACGAG 1 cut(s) 438
Bst4CI ACNGT 5 cut(s) 37, 167, 537, 905, 1283
Bst6I CTCTTC 2 cut(s) 76, 255
BstC8I GCNNGC 2 cut(s) 869, 1186
BstDEI CTNAG 2 cut(s) 136, 1214
BstEII GGTNACC 1 cut(s) 1312
BstF5I GGATG 1 cut(s) 853
BstFNI CGCG 1 cut(s) 1184
BstH2I RGCGCY 1 cut(s) 748
BstHHI GCGC 1 cut(s) 747
BstKTI GATC 3 cut(s) 1006, 1399, 1453
BstMAI GTCTC 2 cut(s) 896, 1378
BstMBI GATC 3 cut(s) 1003, 1396, 1450
BstMWI GCNNNNNNNGC 5 cut(s) 488, 560, 1070, 1079, 1258
BstPI GGTNACC 1 cut(s) 1312
BstSCI CCNGG 1 cut(s) 279
BstSFI CTRYAG 2 cut(s) 297, 904
BstUI CGCG 1 cut(s) 1184
BstV1I GCAGC 6 cut(s) 283, 928, 1032, 1057, 1399, 1421
BstV2I GAAGAC 3 cut(s) 620, 1201, 1340
BstXI CCANNNNNNTGG 2 cut(s) 779, 1309
BstZ17I GTATAC 2 cut(s) 769, 1166
BsuRI GGCC 4 cut(s) 187, 304, 783, 1173
BtsCI GGATG 1 cut(s) 853
BtsIMutI CAGTG 6 cut(s) 255, 556, 894, 981, 999, 1418
BtuMI TCGCGA 1 cut(s) 1184
Cac8I GCNNGC 2 cut(s) 869, 1186
CfoI GCGC 1 cut(s) 747
Cfr13I GGNCC 3 cut(s) 302, 434, 1171
Csp6I GTAC 1 cut(s) 396
CspCI CAANNNNNGTGG 2 cut(s) 455, 490
CviAII CATG 5 cut(s) 374, 393, 429, 544, 673
CviQI GTAC 1 cut(s) 396
DdeI CTNAG 2 cut(s) 136, 1214
DpnI GATC 3 cut(s) 1005, 1398, 1452
DpnII GATC 3 cut(s) 1003, 1396, 1450
EaeI YGGCCR 1 cut(s) 781
Eam1104I CTCTTC 2 cut(s) 76, 255
EarI CTCTTC 2 cut(s) 76, 255
EciI GGCGGA 1 cut(s) 120
Eco130I CCWWGG 2 cut(s) 179, 795
Eco24I GRGCYC 1 cut(s) 232
Eco31I GGTCTC 1 cut(s) 896
Eco32I GATATC 1 cut(s) 1090
Eco47I GGWCC 1 cut(s) 434
Eco57I CTGAAG 4 cut(s) 279, 330, 716, 1241
Eco88I CYCGRG 2 cut(s) 75, 1094
Eco91I GGTNACC 1 cut(s) 1312
EcoO109I RGGNCCY 1 cut(s) 1171
EcoO65I GGTNACC 1 cut(s) 1312
EcoRI GAATTC 1 cut(s) 775
EcoRV GATATC 1 cut(s) 1090
EcoT14I CCWWGG 2 cut(s) 179, 795
EcoT38I GRGCYC 1 cut(s) 232
ErhI CCWWGG 2 cut(s) 179, 795
Esp3I CGTCTC 1 cut(s) 1378
FaeI CATG 5 cut(s) 377, 396, 432, 547, 676
FalI AAGNNNNNCTT 2 cut(s) 114, 146
FaqI GGGAC 1 cut(s) 963
FatI CATG 5 cut(s) 373, 392, 428, 543, 672
FbaI TGATCA 1 cut(s) 1003
FblI GTMKAC 3 cut(s) 539, 768, 1165
Fnu4HI GCNGC 8 cut(s) 185, 297, 743, 942, 1021, 1071, 1410, 1413
FokI GGATG 1 cut(s) 860
FriOI GRGCYC 1 cut(s) 232
Fsp4HI GCNGC 8 cut(s) 185, 297, 743, 942, 1021, 1071, 1410, 1413
FspBI CTAG 4 cut(s) 180, 639, 993, 1220
GlaI GCGC 1 cut(s) 746
GluI GCNGC 8 cut(s) 185, 297, 743, 942, 1021, 1071, 1410, 1413
GsaI CCCAGC 1 cut(s) 1383
HaeII RGCGCY 1 cut(s) 748
HaeIII GGCC 4 cut(s) 187, 304, 783, 1173
HapII CCGG 4 cut(s) 281, 509, 882, 914
HhaI GCGC 1 cut(s) 747
Hin1II CATG 5 cut(s) 377, 396, 432, 547, 676
Hin6I GCGC 1 cut(s) 745
HinP1I GCGC 1 cut(s) 745
HincII GTYRAC 1 cut(s) 1329
HindII GTYRAC 1 cut(s) 1329
HinfI GANTC 7 cut(s) 389, 512, 910, 975, 1196, 1232, 1391
HpaII CCGG 4 cut(s) 281, 509, 882, 914
HphI GGTGA 4 cut(s) 58, 998, 1191, 1306
Hpy166II GTNNAC 6 cut(s) 337, 466, 540, 769, 1166, 1329
Hpy188I TCNGA 5 cut(s) 18, 92, 696, 1217, 1396
Hpy188III TCNNGA 7 cut(s) 75, 233, 334, 509, 882, 1183, 1193
Hpy8I GTNNAC 6 cut(s) 337, 466, 540, 769, 1166, 1329
HpyCH4III ACNGT 5 cut(s) 37, 167, 537, 905, 1283
HpyCH4IV ACGT 1 cut(s) 1032
HpyCH4V TGCA 8 cut(s) 299, 404, 554, 944, 981, 1252, 1415, 1433
HpyF10VI GCNNNNNNNGC 5 cut(s) 488, 560, 1070, 1079, 1258
HpyF3I CTNAG 2 cut(s) 136, 1214
HpySE526I ACGT 1 cut(s) 1032
Hsp92II CATG 5 cut(s) 377, 396, 432, 547, 676
HspAI GCGC 1 cut(s) 745
Kpn2I TCCGGA 2 cut(s) 508, 881
Ksp22I TGATCA 1 cut(s) 1003
Kzo9I GATC 3 cut(s) 1003, 1396, 1450
LmnI GCTCC 5 cut(s) 70, 235, 642, 1266, 1294
Lsp1109I GCAGC 6 cut(s) 283, 928, 1032, 1057, 1399, 1421
LweI GCATC 2 cut(s) 52, 1424
MaeI CTAG 4 cut(s) 180, 639, 993, 1220
MaeII ACGT 1 cut(s) 1032
MaeIII GTNAC 7 cut(s) 46, 167, 381, 854, 997, 1028, 1312
MalI GATC 3 cut(s) 1005, 1398, 1452
MboI GATC 3 cut(s) 1003, 1396, 1450
MboII GAAGA 8 cut(s) 63, 272, 620, 689, 1138, 1201, 1252, 1340
MfeI CAATTG 2 cut(s) 99, 456
MhlI GDGCHC 2 cut(s) 232, 647
MlsI TGGCCA 1 cut(s) 783
MluNI TGGCCA 1 cut(s) 783
MlyI GAGTC 3 cut(s) 398, 506, 1226
MmeI TCCRAC 1 cut(s) 267
Mox20I TGGCCA 1 cut(s) 783
MroI TCCGGA 2 cut(s) 508, 881
MscI TGGCCA 1 cut(s) 783
MseI TTAA 5 cut(s) 191, 683, 732, 1289, 1459
MslI CAYNNNNRTG 1 cut(s) 1307
Msp20I TGGCCA 1 cut(s) 783
MspA1I CMGCKG 2 cut(s) 1379, 1412
MspI CCGG 4 cut(s) 281, 509, 882, 914
MspR9I CCNGG 1 cut(s) 281
MunI CAATTG 2 cut(s) 99, 456
Mva1269I GAATGC 2 cut(s) 34, 1297
MvnI CGCG 1 cut(s) 1184
MwoI GCNNNNNNNGC 5 cut(s) 488, 560, 1070, 1079, 1258
NciI CCSGG 1 cut(s) 281
NdeII GATC 3 cut(s) 1003, 1396, 1450
NlaIII CATG 5 cut(s) 377, 396, 432, 547, 676
NlaIV GGNNCC 2 cut(s) 231, 1172
NmuCI GTSAC 3 cut(s) 46, 997, 1312
NruI TCGCGA 1 cut(s) 1184
PaeR7I CTCGAG 2 cut(s) 75, 1094
PctI GAATGC 2 cut(s) 34, 1297
PfeI GAWTC 4 cut(s) 910, 975, 1196, 1391
PkrI GCNGC 8 cut(s) 186, 298, 744, 943, 1022, 1072, 1411, 1414
PleI GAGTC 3 cut(s) 397, 506, 1226
PpsI GAGTC 3 cut(s) 397, 506, 1226
PspEI GGTNACC 1 cut(s) 1312
PspFI CCCAGC 1 cut(s) 1379
PspN4I GGNNCC 2 cut(s) 231, 1172
PspPI GGNCC 3 cut(s) 302, 434, 1171
PspXI VCTCGAGB 1 cut(s) 1094
PstI CTGCAG 1 cut(s) 301
PvuII CAGCTG 1 cut(s) 1412
RruI TCGCGA 1 cut(s) 1184
RsaI GTAC 1 cut(s) 397
RsaNI GTAC 1 cut(s) 396
RseI CAYNNNNRTG 1 cut(s) 1307
SaqAI TTAA 5 cut(s) 191, 683, 732, 1289, 1459
SatI GCNGC 8 cut(s) 185, 297, 743, 942, 1021, 1071, 1410, 1413
Sau3AI GATC 3 cut(s) 1003, 1396, 1450
Sau96I GGNCC 3 cut(s) 302, 434, 1171
SchI GAGTC 3 cut(s) 398, 506, 1226
ScrFI CCNGG 1 cut(s) 281
SduI GDGCHC 2 cut(s) 232, 647
SfaNI GCATC 2 cut(s) 52, 1424
SfcI CTRYAG 2 cut(s) 297, 904
Sfr274I CTCGAG 2 cut(s) 75, 1094
SinI GGWCC 1 cut(s) 434
SlaI CTCGAG 2 cut(s) 75, 1094
SmiMI CAYNNNNRTG 1 cut(s) 1307
SmlI CTYRAG 4 cut(s) 75, 386, 1094, 1403
SmoI CTYRAG 4 cut(s) 75, 386, 1094, 1403
SsiI CCGC 4 cut(s) 105, 184, 742, 1377
SspI AATATT 1 cut(s) 1273
SspMI CTAG 4 cut(s) 180, 639, 993, 1220
StyD4I CCNGG 1 cut(s) 279
StyI CCWWGG 2 cut(s) 179, 795
TaaI ACNGT 5 cut(s) 37, 167, 537, 905, 1283
TaiI ACGT 1 cut(s) 1035
TaqI TCGA 2 cut(s) 76, 1095
TauI GCSGC 2 cut(s) 187, 745
TfiI GAWTC 4 cut(s) 910, 975, 1196, 1391
Tru1I TTAA 5 cut(s) 191, 683, 732, 1289, 1459
Tru9I TTAA 5 cut(s) 191, 683, 732, 1289, 1459
TscAI CASTG 6 cut(s) 262, 556, 901, 988, 1006, 1425
TseFI GTSAC 3 cut(s) 46, 997, 1312
TseI GCWGC 6 cut(s) 296, 941, 1020, 1070, 1409, 1412
Tsp45I GTSAC 3 cut(s) 46, 997, 1312
TspDTI ATGAA 5 cut(s) 288, 417, 612, 620, 1260
TspRI CASTG 6 cut(s) 262, 556, 901, 988, 1006, 1425
VpaK11BI GGWCC 1 cut(s) 434
XapI RAATTY 2 cut(s) 690, 775
XhoI CTCGAG 2 cut(s) 75, 1094
XmaJI CCTAGG 1 cut(s) 179
XmiI GTMKAC 3 cut(s) 539, 768, 1165
XspI CTAG 4 cut(s) 180, 639, 993, 1220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.