MD15G1077700.v1.1

Long chain base biosynthesis protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004129
Physical Location & Seq
Reverse (-)
5300794 .. 5306026
5233 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1077700.v1.1.491

Sequence Viewer

Length: 1461 bp
ATGTCATCAAGTGTTATGGACATAGTGAATGCTACTGTAGATTGGGTGACGGCTACTTTAGATGCTCCCTCTTCTCGAGCTGTTATTTTTGGATTTCCTATTGGCGGACATTTATTTGTAGAAGTATTGCTTTTCTTAGTCATTGTTTATCTATTTTCCCAGAACAGTTACAGACCGCCTAGGCGGCCTTTAACAGAGCAGGAAAAGGACGAGCTATGTGAGGAATGGGTTCCTGACCCTCTTATTCCTTCTATCACTGATGAGATGCTGTATGAACCCCCCGTGTTGGAAAGTGCTGCAGGGCCACATACAATAATTAATGGTAAAGAAGTCGTGAACTTTGCTTCAGCAAATTATCTGGGATTGATAGGACATGAGAAGTTACTTGAGTCATGTAACTCTGCATTGGAAAAATATGGTGTTGGCTCATGTGGTCCTCGTGGGTTTTATGGAACAATTGATGTCCACCTTGACTGTGAGGCGAGAATTGCAAACTTTTTGGGAACTCCCGACTCAATTCTGTATTCTTATGGACTGTCGACCATGTTCAGTGCAATTCCAGCATTTGCCAAGAAAGGAGATATTATTGTTGTAGATGAGGGTGTCCATTGGGGAATACAAAATGGTCTTCATCTTTCTAGAAGCACAATCAAGTATTTCAAGCACAACGACATGGAAGATTTAAGGAAAATTCTGGAGGAACTCACTTCAAATAAGAAAAGTGGGCGAGTTAAGAAATTGCGGCGCTACATTGTAGTTGAAGCTGTATACCAGAATTCTGGCCAAATAGCCCCCTTAGACAAGATAGTTGAATTGAAGGAGAAATATAAGTTTCGTGTGTTATTGGATGAGAGTAACTCATTTGGCGTGCTTGGAAAATCTGGAAGAGGTCTAACTGAATACTGCGGGATTCCGGTTGAAAAAATAGATATTATTACTGCTGCAATGGGACACGCATTAGCCACAGAAGGAGGATTCTGCACTGGAAGTGCTAGAGTCACCGATCACCAACGATTGAGTAGCTCTGGGTACGTCTTTTCTGCTTCTTTGCCCCCATATCTTGCAAGTGCTGCCATTACTGCCATTGATATCCTCGAGGAAAACCCTGGTCTGATAACAAAGCTGAAGAAAAATATTGCCATATTATGGAAAGGATTGTCAGGTATACAGGGCCTCTCATTCGCTAGCAGTCCAGAATCCCCGATTGTCTTCCTCAGACTAGAGAAAGCAGATTCTGTGAAGAGTGAACTGCAACTCAAACTCCTTCAAGATATTGCCAACCGTTTGTTGAAGGAGCATTCCATCTTTGTGGTCGCCTCCAAAAAGTCAACACTTGATAAAAGTCGTCTTCCATTAGGAATTAGATTGTTCGTCTCCTCAGGCCATACGGAATCTGATCTGCTCAAGGCAGCTGCATCACTGAAAGGTGTTGCAGAATTGGTGCTGAAGGATCATATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0002178 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006066 GO:0006629 GO:0006643 GO:0006644 GO:0006665 GO:0006667 GO:0006670 GO:0006672 GO:0006684 GO:0006686 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009058 GO:0009790 GO:0009791 GO:0009793 GO:0009825 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010154 GO:0010506 GO:0010508 GO:0010941 GO:0012505 GO:0016020 GO:0016049 GO:0016239 GO:0016241 GO:0017059 GO:0019222 GO:0019637 GO:0019751 GO:0022414 GO:0030148 GO:0031211 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031984 GO:0032501 GO:0032502 GO:0032991 GO:0034311 GO:0034312 GO:0034641 GO:0035339 GO:0040007 GO:0042175 GO:0043067 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046165 GO:0046173 GO:0046467 GO:0046511 GO:0046512 GO:0046513 GO:0046519 GO:0046520 GO:0048316 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0061458 GO:0065007 GO:0071704 GO:0090407 GO:0097164 GO:0098796 GO:0098827 GO:1901564 GO:1901566 GO:1901576 GO:1901615 GO:1901617 GO:1902494 GO:1904502 GO:1904504 GO:1905961 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00600 ko01100 ko04071 ko04138 map00600 map01100 map04071 map04138
Pfam Domains
Protein Families
Aminotran_1_2

Protein Analysis

487

Amino Acids

53.21

Weight (kDa)

6.34

Isoelectric Point (pI)

32.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 110 - 473 4.1e-36 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 1094
AccB7I CCANNNNNTGG 1 cut(s) 1146
AccI GTMKAC 3 cut(s) 539, 768, 1165
AciI CCGC 5 cut(s) 105, 176, 184, 742, 906
AclWI GGATC 1 cut(s) 1458
AcoI YGGCCR 1 cut(s) 781
AcsI RAATTY 2 cut(s) 690, 775
AcuI CTGAAG 2 cut(s) 330, 1145
AfaI GTAC 1 cut(s) 1031
AfiI CCNNNNNNNGG 3 cut(s) 104, 286, 1146
AgsI TTSAA 8 cut(s) 661, 711, 761, 812, 817, 920, 1268, 1291
AjnI CCWGG 1 cut(s) 1105
AluBI AGCT 6 cut(s) 80, 214, 764, 1023, 1123, 1412
AluI AGCT 6 cut(s) 80, 214, 764, 1023, 1123, 1412
Alw26I GTCTC 1 cut(s) 1378
AlwI GGATC 1 cut(s) 1458
AlwNI CAGNNNCTG 1 cut(s) 1235
Ama87I CYCGRG 2 cut(s) 75, 1094
AoxI GGCC 5 cut(s) 185, 302, 781, 1171, 1381
ApeKI GCWGC 5 cut(s) 296, 941, 1070, 1409, 1412
ApoI RAATTY 2 cut(s) 690, 775
AseI ATTAAT 1 cut(s) 318
Asp700I GAANNNNTTC 1 cut(s) 228
AspA2I CCTAGG 1 cut(s) 179
AspLEI GCGC 1 cut(s) 747
AspS9I GGNCC 3 cut(s) 302, 434, 1171
AsuHPI GGTGA 3 cut(s) 58, 991, 998
AsuNHI GCTAGC 1 cut(s) 1184
AvaI CYCGRG 2 cut(s) 75, 1094
AvaII GGWCC 1 cut(s) 434
AvrII CCTAGG 1 cut(s) 179
AxyI CCTNAGG 1 cut(s) 1378
BalI TGGCCA 1 cut(s) 783
BauI CACGAG 1 cut(s) 438
BbsI GAAGAC 3 cut(s) 620, 1201, 1340
BbvI GCAGC 5 cut(s) 283, 928, 1057, 1399, 1421
BccI CCATC 1 cut(s) 1310
BceAI ACGGC 1 cut(s) 66
BciT130I CCWGG 1 cut(s) 1107
BcoDI GTCTC 1 cut(s) 1378
BfaI CTAG 5 cut(s) 180, 639, 993, 1185, 1220
BfmI CTRYAG 2 cut(s) 36, 297
BfoI RGCGCY 1 cut(s) 748
BglI GCCNNNNNGGC 1 cut(s) 184
BisI GCNGC 7 cut(s) 185, 297, 743, 942, 1071, 1410, 1413
BlnI CCTAGG 1 cut(s) 179
BlsI GCNGC 7 cut(s) 186, 298, 744, 943, 1072, 1411, 1414
Bme1390I CCNGG 1 cut(s) 1107
Bme18I GGWCC 1 cut(s) 434
BmeT110I CYCGRG 2 cut(s) 75, 1094
BmgT120I GGNCC 3 cut(s) 302, 434, 1171
BmiI GGNNCC 1 cut(s) 231
BmrFI CCNGG 1 cut(s) 1107
BmsI GCATC 3 cut(s) 52, 255, 1424
BmtI GCTAGC 1 cut(s) 1188
BpiI GAAGAC 3 cut(s) 620, 1201, 1340
BplI GAGNNNNNCTC 2 cut(s) 842, 874
BpmI CTGGAG 1 cut(s) 716
BpuEI CTTGAG 2 cut(s) 407, 1388
BsaJI CCNNGG 2 cut(s) 179, 1105
BsaWI WCCGGW 1 cut(s) 913
BsaXI ACNNNNNCTCC 2 cut(s) 1245, 1275
Bsc4I CCNNNNNNNGG 3 cut(s) 104, 286, 1146
Bse1I ACTGG 1 cut(s) 988
Bse21I CCTNAGG 1 cut(s) 1378
Bse3DI GCAATG 1 cut(s) 951
BseBI CCWGG 1 cut(s) 1107
BseDI CCNNGG 2 cut(s) 179, 1105
BseGI GGATG 1 cut(s) 853
BseLI CCNNNNNNNGG 3 cut(s) 104, 286, 1146
BseMI GCAATG 1 cut(s) 951
BseMII CTCAG 2 cut(s) 1228, 1392
BseNI ACTGG 1 cut(s) 988
BseRI GAGGAG 1 cut(s) 1366
BseXI GCAGC 5 cut(s) 283, 928, 1057, 1399, 1421
BsgI GTGCAG 1 cut(s) 964
BshFI GGCC 5 cut(s) 187, 304, 783, 1173, 1383
BsiHKCI CYCGRG 2 cut(s) 75, 1094
BsiSI CCGG 1 cut(s) 914
BslFI GGGAC 1 cut(s) 963
BslI CCNNNNNNNGG 3 cut(s) 104, 286, 1146
BsmAI GTCTC 1 cut(s) 1378
BsmBI CGTCTC 1 cut(s) 1378
BsmFI GGGAC 1 cut(s) 963
BsmI GAATGC 2 cut(s) 34, 1297
BsnI GGCC 5 cut(s) 187, 304, 783, 1173, 1383
BsoBI CYCGRG 2 cut(s) 75, 1094
Bsp143I GATC 3 cut(s) 1003, 1396, 1450
BspACI CCGC 5 cut(s) 105, 176, 184, 742, 906
BspANI GGCC 5 cut(s) 187, 304, 783, 1173, 1383
BspCNI CTCAG 2 cut(s) 1227, 1391
BspLI GGNNCC 1 cut(s) 231
BspMAI CTGCAG 1 cut(s) 301
BspOI GCTAGC 1 cut(s) 1188
BspPI GGATC 1 cut(s) 1458
BsrDI GCAATG 1 cut(s) 951
BsrI ACTGG 1 cut(s) 988
BssECI CCNNGG 2 cut(s) 179, 1105
BssMI GATC 3 cut(s) 1003, 1396, 1450
BssNAI GTATAC 2 cut(s) 769, 1166
BssSI CACGAG 1 cut(s) 438
BssT1I CCWWGG 1 cut(s) 179
Bst1107I GTATAC 2 cut(s) 769, 1166
Bst2BI CACGAG 1 cut(s) 438
Bst2UI CCWGG 1 cut(s) 1107
Bst4CI ACNGT 5 cut(s) 37, 167, 476, 537, 1283
Bst6I CTCTTC 3 cut(s) 76, 880, 1235
BstAPI GCANNNNNTGC 1 cut(s) 1070
BstC8I GCNNGC 2 cut(s) 869, 1186
BstDEI CTNAG 4 cut(s) 136, 796, 1214, 1378
BstF5I GGATG 1 cut(s) 853
BstH2I RGCGCY 1 cut(s) 748
BstHHI GCGC 1 cut(s) 747
BstKTI GATC 3 cut(s) 1006, 1399, 1453
BstMAI GTCTC 1 cut(s) 1378
BstMBI GATC 3 cut(s) 1003, 1396, 1450
BstMWI GCNNNNNNNGC 5 cut(s) 184, 488, 560, 1070, 1079
BstNI CCWGG 1 cut(s) 1107
BstSCI CCNGG 1 cut(s) 1105
BstSFI CTRYAG 2 cut(s) 36, 297
BstV1I GCAGC 5 cut(s) 283, 928, 1057, 1399, 1421
BstV2I GAAGAC 3 cut(s) 620, 1201, 1340
BstXI CCANNNNNNTGG 2 cut(s) 779, 1309
BstZ17I GTATAC 2 cut(s) 769, 1166
Bsu36I CCTNAGG 1 cut(s) 1378
BsuRI GGCC 5 cut(s) 187, 304, 783, 1173, 1383
BtsCI GGATG 1 cut(s) 853
BtsIMutI CAGTG 4 cut(s) 255, 556, 981, 1418
Cac8I GCNNGC 2 cut(s) 869, 1186
CaiI CAGNNNCTG 1 cut(s) 1235
CfoI GCGC 1 cut(s) 747
Cfr13I GGNCC 3 cut(s) 302, 434, 1171
Csp6I GTAC 1 cut(s) 1030
CviAII CATG 5 cut(s) 374, 393, 429, 544, 673
CviQI GTAC 1 cut(s) 1030
DdeI CTNAG 4 cut(s) 136, 796, 1214, 1378
DpnI GATC 3 cut(s) 1005, 1398, 1452
DpnII GATC 3 cut(s) 1003, 1396, 1450
EaeI YGGCCR 1 cut(s) 781
Eam1104I CTCTTC 3 cut(s) 76, 880, 1235
EarI CTCTTC 3 cut(s) 76, 880, 1235
EciI GGCGGA 1 cut(s) 120
Eco130I CCWWGG 1 cut(s) 179
Eco32I GATATC 1 cut(s) 1090
Eco47I GGWCC 1 cut(s) 434
Eco57I CTGAAG 2 cut(s) 330, 1145
Eco81I CCTNAGG 1 cut(s) 1378
Eco88I CYCGRG 2 cut(s) 75, 1094
EcoO109I RGGNCCY 1 cut(s) 1171
EcoRI GAATTC 1 cut(s) 775
EcoRII CCWGG 1 cut(s) 1105
EcoRV GATATC 1 cut(s) 1090
EcoT14I CCWWGG 1 cut(s) 179
ErhI CCWWGG 1 cut(s) 179
Esp3I CGTCTC 1 cut(s) 1378
FaeI CATG 5 cut(s) 377, 396, 432, 547, 676
FalI AAGNNNNNCTT 2 cut(s) 114, 146
FaqI GGGAC 1 cut(s) 963
FatI CATG 5 cut(s) 373, 392, 428, 543, 672
FauI CCCGC 1 cut(s) 899
FblI GTMKAC 3 cut(s) 539, 768, 1165
Fnu4HI GCNGC 7 cut(s) 185, 297, 743, 942, 1071, 1410, 1413
FokI GGATG 1 cut(s) 860
Fsp4HI GCNGC 7 cut(s) 185, 297, 743, 942, 1071, 1410, 1413
FspBI CTAG 5 cut(s) 180, 639, 993, 1185, 1220
GlaI GCGC 1 cut(s) 746
GluI GCNGC 7 cut(s) 185, 297, 743, 942, 1071, 1410, 1413
GsuI CTGGAG 1 cut(s) 716
HaeII RGCGCY 1 cut(s) 748
HaeIII GGCC 5 cut(s) 187, 304, 783, 1173, 1383
HapII CCGG 1 cut(s) 914
HhaI GCGC 1 cut(s) 747
Hin1II CATG 5 cut(s) 377, 396, 432, 547, 676
Hin6I GCGC 1 cut(s) 745
HinP1I GCGC 1 cut(s) 745
HincII GTYRAC 2 cut(s) 540, 1329
HindII GTYRAC 2 cut(s) 540, 1329
HinfI GANTC 8 cut(s) 389, 512, 910, 975, 996, 1196, 1232, 1391
HpaII CCGG 1 cut(s) 914
HphI GGTGA 3 cut(s) 58, 991, 998
Hpy166II GTNNAC 7 cut(s) 337, 466, 540, 769, 1166, 1247, 1329
Hpy188I TCNGA 3 cut(s) 1113, 1217, 1396
Hpy188III TCNNGA 9 cut(s) 75, 233, 334, 509, 639, 695, 882, 1193, 1268
Hpy8I GTNNAC 7 cut(s) 337, 466, 540, 769, 1166, 1247, 1329
HpyAV CCTTC 6 cut(s) 258, 811, 962, 1274, 1285, 1441
HpyCH4III ACNGT 5 cut(s) 37, 167, 476, 537, 1283
HpyCH4IV ACGT 1 cut(s) 1032
HpyF10VI GCNNNNNNNGC 5 cut(s) 184, 488, 560, 1070, 1079
HpyF3I CTNAG 4 cut(s) 136, 796, 1214, 1378
HpySE526I ACGT 1 cut(s) 1032
Hsp92II CATG 5 cut(s) 377, 396, 432, 547, 676
HspAI GCGC 1 cut(s) 745
Kzo9I GATC 3 cut(s) 1003, 1396, 1450
LmnI GCTCC 2 cut(s) 70, 1294
Lsp1109I GCAGC 5 cut(s) 283, 928, 1057, 1399, 1421
LweI GCATC 3 cut(s) 52, 255, 1424
MaeI CTAG 5 cut(s) 180, 639, 993, 1185, 1220
MaeII ACGT 1 cut(s) 1032
MaeIII GTNAC 6 cut(s) 46, 167, 381, 395, 854, 997
MalI GATC 3 cut(s) 1005, 1398, 1452
MboI GATC 3 cut(s) 1003, 1396, 1450
MboII GAAGA 8 cut(s) 63, 620, 689, 897, 1138, 1201, 1252, 1340
MfeI CAATTG 1 cut(s) 456
MlsI TGGCCA 1 cut(s) 783
MluNI TGGCCA 1 cut(s) 783
MlyI GAGTC 3 cut(s) 398, 506, 1005
MmeI TCCRAC 1 cut(s) 267
Mox20I TGGCCA 1 cut(s) 783
MroXI GAANNNNTTC 1 cut(s) 228
MscI TGGCCA 1 cut(s) 783
MseI TTAA 4 cut(s) 191, 318, 683, 732
MslI CAYNNNNRTG 1 cut(s) 1307
Msp20I TGGCCA 1 cut(s) 783
MspA1I CMGCKG 1 cut(s) 1412
MspI CCGG 1 cut(s) 914
MspR9I CCNGG 1 cut(s) 1107
MunI CAATTG 1 cut(s) 456
Mva1269I GAATGC 2 cut(s) 34, 1297
MvaI CCWGG 1 cut(s) 1107
MwoI GCNNNNNNNGC 5 cut(s) 184, 488, 560, 1070, 1079
NdeII GATC 3 cut(s) 1003, 1396, 1450
NheI GCTAGC 1 cut(s) 1184
NlaIII CATG 5 cut(s) 377, 396, 432, 547, 676
NlaIV GGNNCC 1 cut(s) 231
NmuCI GTSAC 2 cut(s) 46, 997
PaeR7I CTCGAG 2 cut(s) 75, 1094
PctI GAATGC 2 cut(s) 34, 1297
PdmI GAANNNNTTC 1 cut(s) 228
PfeI GAWTC 5 cut(s) 910, 975, 1196, 1232, 1391
PflMI CCANNNNNTGG 1 cut(s) 1146
PkrI GCNGC 7 cut(s) 186, 298, 744, 943, 1072, 1411, 1414
PleI GAGTC 3 cut(s) 397, 506, 1004
PpsI GAGTC 3 cut(s) 397, 506, 1004
PshBI ATTAAT 1 cut(s) 318
Psp6I CCWGG 1 cut(s) 1105
PspGI CCWGG 1 cut(s) 1105
PspN4I GGNNCC 1 cut(s) 231
PspPI GGNCC 3 cut(s) 302, 434, 1171
PspXI VCTCGAGB 1 cut(s) 1094
PstI CTGCAG 1 cut(s) 301
PstNI CAGNNNCTG 1 cut(s) 1235
PvuII CAGCTG 1 cut(s) 1412
RsaI GTAC 1 cut(s) 1031
RsaNI GTAC 1 cut(s) 1030
RseI CAYNNNNRTG 1 cut(s) 1307
SalI GTCGAC 1 cut(s) 538
SaqAI TTAA 4 cut(s) 191, 318, 683, 732
SatI GCNGC 7 cut(s) 185, 297, 743, 942, 1071, 1410, 1413
Sau3AI GATC 3 cut(s) 1003, 1396, 1450
Sau96I GGNCC 3 cut(s) 302, 434, 1171
SchI GAGTC 3 cut(s) 398, 506, 1005
ScrFI CCNGG 1 cut(s) 1107
SfaNI GCATC 3 cut(s) 52, 255, 1424
SfcI CTRYAG 2 cut(s) 36, 297
Sfr274I CTCGAG 2 cut(s) 75, 1094
SinI GGWCC 1 cut(s) 434
SlaI CTCGAG 2 cut(s) 75, 1094
SmiMI CAYNNNNRTG 1 cut(s) 1307
SmlI CTYRAG 4 cut(s) 75, 386, 1094, 1403
SmoI CTYRAG 4 cut(s) 75, 386, 1094, 1403
SsiI CCGC 5 cut(s) 105, 176, 184, 742, 906
SspI AATATT 1 cut(s) 1135
SspMI CTAG 5 cut(s) 180, 639, 993, 1185, 1220
StyD4I CCNGG 1 cut(s) 1105
StyI CCWWGG 1 cut(s) 179
TaaI ACNGT 5 cut(s) 37, 167, 476, 537, 1283
TaiI ACGT 1 cut(s) 1035
TaqI TCGA 3 cut(s) 76, 539, 1095
TauI GCSGC 2 cut(s) 187, 745
TfiI GAWTC 5 cut(s) 910, 975, 1196, 1232, 1391
Tru1I TTAA 4 cut(s) 191, 318, 683, 732
Tru9I TTAA 4 cut(s) 191, 318, 683, 732
TscAI CASTG 4 cut(s) 262, 556, 988, 1425
TseFI GTSAC 2 cut(s) 46, 997
TseI GCWGC 5 cut(s) 296, 941, 1070, 1409, 1412
Tsp45I GTSAC 2 cut(s) 46, 997
TspDTI ATGAA 2 cut(s) 288, 620
TspGWI ACGGA 1 cut(s) 1403
TspRI CASTG 4 cut(s) 262, 556, 988, 1425
Van91I CCANNNNNTGG 1 cut(s) 1146
VpaK11BI GGWCC 1 cut(s) 434
VspI ATTAAT 1 cut(s) 318
XapI RAATTY 2 cut(s) 690, 775
XbaI TCTAGA 1 cut(s) 638
XhoI CTCGAG 2 cut(s) 75, 1094
XmaJI CCTAGG 1 cut(s) 179
XmiI GTMKAC 3 cut(s) 539, 768, 1165
XmnI GAANNNNTTC 1 cut(s) 228
XspI CTAG 5 cut(s) 180, 639, 993, 1185, 1220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.