MD08G1119000.v1.1

GTP-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0009883
Physical Location & Seq
Forward (+)
10849146 .. 10851486
2341 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1119000.v1.1.491

Sequence Viewer

Length: 969 bp
ATGGTTCTGCTTCACCTCCCAAGAACCCACATATCCCTCTCCACCCATTTCTCTCTATTCCCAAAACCCAAAACCCCCGCCTTCGCCACCGCTACAACTCTCCCCCCAATCCTCACCTCCGTCCTCTTCTCCGCCCCCAAATCCACCCTCTCGGCCTCCGAACCCATACCCATTTCCGAATTGCCGGAACCCCAAATCGAACCCCCTCAGGAATTCGAAACCCACATCGAAATCCCGCTCGAGAAGCTCTTCGTGCCGCCGGAAACCGAGGTGTCGTACACCGACGCCGGTGCTCTGAGCACCCGAATCTTGAAAGGGTCCAATATTCTGCTCAGTAAGTACGCCAGGAACGCTCAGGTGGAGCAGGCCGAGTTCGTGAAGAGCAGTGTGAGGACTGAGGATTGCCCCTCTGATGGTCTCCCGGAGTTTGCTCTCGTGGGTCGGTCCAATGTCGGCAAGTCCTCGCTGCTCAACTCGCTCGTGCGACGGAAGCGCCTCGCTTTGACGTCCAAGAAACGGAAAACACAATGTATCAATCATTTTCGGATCAATGATGGCTGGTACCTGGTGGATTTGCCAGGCTACGGGTATGCAGCTGCACCACAGGAACTTAGAACTGACTGGGTTAAGTTTACTAAAGACTACTTCCTGAACAGGTCGACATTAGTTTCGGTTTTCCTTCTCATCGATGCCAGCATTCCTGCCAAAAAAATTGATCTGGAGTATGCTAGTTGGCTGGGTCAGAATCAGATTCCTATGACTTTGATATTCACCAAATGTGACAAGCGGAAAAAGAAGAGGCACGGAGGGAAGAAAGCAGAAGAAAATGTGCAGGATTTCCAGGAGTTAATAAGCGGCTTCTTCGAGACCGTGCCTCCATGGATTATGACCAGCAGCCTGACCAATCAGGGTCGTGATGAGATGCTATTACATATGGCTCAGCTGCGGAACTACTGGCTGAAGCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MMR_HSR1

Protein Analysis

323

Amino Acids

36.43

Weight (kDa)

9.18

Isoelectric Point (pI)

50.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MMR_HSR1 PF01926 143 - 259 6e-16 50S ribosome-binding GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 509
Acc65I GGTACC 1 cut(s) 561
AccB1I GGYRCC 1 cut(s) 561
AccBSI CCGCTC 1 cut(s) 238
AccI GTMKAC 1 cut(s) 659
AciI CCGC 8 cut(s) 78, 90, 132, 236, 257, 787, 855, 946
AclWI GGATC 1 cut(s) 554
AcsI RAATTY 1 cut(s) 212
AcyI GRCGYC 2 cut(s) 285, 506
AfaI GTAC 3 cut(s) 278, 341, 563
AfiI CCNNNNNNNGG 3 cut(s) 413, 516, 584
AgsI TTSAA 1 cut(s) 313
AjnI CCWGG 4 cut(s) 344, 564, 577, 840
AluBI AGCT 3 cut(s) 247, 596, 943
AluI AGCT 3 cut(s) 247, 596, 943
Alw21I GWGCWC 2 cut(s) 295, 302
Alw26I GTCTC 2 cut(s) 422, 860
AlwI GGATC 1 cut(s) 554
Ama87I CYCGRG 1 cut(s) 239
AoxI GGCC 2 cut(s) 153, 366
ApeKI GCWGC 5 cut(s) 466, 593, 596, 894, 943
ApoI RAATTY 1 cut(s) 212
ArsI GACNNNNNNTTYG 2 cut(s) 652, 684
Asp700I GAANNNNTTC 1 cut(s) 248
Asp718I GGTACC 1 cut(s) 561
AspLEI GCGC 1 cut(s) 495
AspS9I GGNCC 2 cut(s) 318, 444
AsuC2I CCSGG 1 cut(s) 422
AsuHPI GGTGA 3 cut(s) 5, 106, 763
AsuII TTCGAA 1 cut(s) 216
AvaI CYCGRG 1 cut(s) 239
AvaII GGWCC 2 cut(s) 318, 444
AxyI CCTNAGG 1 cut(s) 207
BanI GGYRCC 1 cut(s) 561
BauI CACGAG 2 cut(s) 434, 479
Bbv12I GWGCWC 2 cut(s) 295, 302
BbvI GCAGC 5 cut(s) 453, 583, 605, 906, 930
BccI CCATC 2 cut(s) 407, 548
BcgI CGANNNNNNTGC 2 cut(s) 272, 306
BciT130I CCWGG 4 cut(s) 346, 566, 579, 842
BcnI CCSGG 1 cut(s) 422
BcoDI GTCTC 2 cut(s) 422, 860
BfaI CTAG 2 cut(s) 729, 967
BfoI RGCGCY 1 cut(s) 496
BisI GCNGC 7 cut(s) 257, 467, 594, 597, 856, 895, 944
BlpI GCTNAGC 1 cut(s) 939
BlsI GCNGC 7 cut(s) 258, 468, 595, 598, 857, 896, 945
Bme1390I CCNGG 5 cut(s) 346, 422, 566, 579, 842
Bme18I GGWCC 2 cut(s) 318, 444
BmeT110I CYCGRG 1 cut(s) 239
BmgT120I GGNCC 2 cut(s) 318, 444
BmiI GGNNCC 3 cut(s) 189, 319, 563
BmrFI CCNGG 5 cut(s) 346, 422, 566, 579, 842
BmrI ACTGGG 1 cut(s) 631
BmsI GCATC 2 cut(s) 679, 912
BmuI ACTGGG 1 cut(s) 631
BpmI CTGGAG 1 cut(s) 740
Bpu10I CCTNAGC 1 cut(s) 354
Bpu1102I GCTNAGC 1 cut(s) 939
Bpu14I TTCGAA 1 cut(s) 216
BpuMI CCSGG 1 cut(s) 422
Bsa29I ATCGAT 1 cut(s) 687
BsaHI GRCGYC 2 cut(s) 285, 506
BsaI GGTCTC 2 cut(s) 422, 860
BsaJI CCNNGG 2 cut(s) 267, 878
BsaXI ACNNNNNCTCC 2 cut(s) 859, 889
Bsc4I CCNNNNNNNGG 3 cut(s) 413, 516, 584
Bse118I RCCGGY 1 cut(s) 287
Bse1I ACTGG 2 cut(s) 626, 959
Bse21I CCTNAGG 1 cut(s) 207
BseBI CCWGG 4 cut(s) 346, 566, 579, 842
BseCI ATCGAT 1 cut(s) 687
BseDI CCNNGG 2 cut(s) 267, 878
BseLI CCNNNNNNNGG 3 cut(s) 413, 516, 584
BseMII CTCAG 6 cut(s) 221, 287, 346, 368, 387, 953
BseNI ACTGG 2 cut(s) 626, 959
BseXI GCAGC 5 cut(s) 453, 583, 605, 906, 930
BseYI CCCAGC 1 cut(s) 736
BsgI GTGCAG 2 cut(s) 582, 851
BshFI GGCC 2 cut(s) 155, 368
BshNI GGYRCC 1 cut(s) 561
BshVI ATCGAT 1 cut(s) 687
BsiHKAI GWGCWC 2 cut(s) 295, 302
BsiHKCI CYCGRG 1 cut(s) 239
BsiSI CCGG 4 cut(s) 185, 260, 288, 422
BslI CCNNNNNNNGG 3 cut(s) 413, 516, 584
BsmAI GTCTC 2 cut(s) 422, 860
BsmI GAATGC 1 cut(s) 696
BsnI GGCC 2 cut(s) 155, 368
Bso31I GGTCTC 2 cut(s) 422, 860
BsoBI CYCGRG 1 cut(s) 239
Bsp119I TTCGAA 1 cut(s) 216
Bsp1286I GDGCHC 2 cut(s) 295, 302
Bsp143I GATC 2 cut(s) 546, 715
Bsp1720I GCTNAGC 1 cut(s) 939
Bsp19I CCATGG 1 cut(s) 878
BspACI CCGC 8 cut(s) 78, 90, 132, 236, 257, 787, 855, 946
BspANI GGCC 2 cut(s) 155, 368
BspCNI CTCAG 6 cut(s) 220, 288, 345, 367, 388, 952
BspDI ATCGAT 1 cut(s) 687
BspLI GGNNCC 3 cut(s) 189, 319, 563
BspPI GGATC 1 cut(s) 554
BspQI GCTCTTC 2 cut(s) 254, 374
BspT104I TTCGAA 1 cut(s) 216
BspT107I GGYRCC 1 cut(s) 561
BspTNI GGTCTC 2 cut(s) 422, 860
BsrBI CCGCTC 1 cut(s) 238
BsrFI RCCGGY 1 cut(s) 287
BsrI ACTGG 2 cut(s) 626, 959
BssAI RCCGGY 1 cut(s) 287
BssECI CCNNGG 2 cut(s) 267, 878
BssMI GATC 2 cut(s) 546, 715
BssNI GRCGYC 2 cut(s) 285, 506
BssSI CACGAG 2 cut(s) 434, 479
BssT1I CCWWGG 1 cut(s) 878
Bst2BI CACGAG 2 cut(s) 434, 479
Bst2UI CCWGG 4 cut(s) 346, 566, 579, 842
Bst4CI ACNGT 1 cut(s) 871
Bst6I CTCTTC 4 cut(s) 131, 254, 374, 791
BstACI GRCGYC 2 cut(s) 285, 506
BstBI TTCGAA 1 cut(s) 216
BstC8I GCNNGC 2 cut(s) 366, 694
BstDEI CTNAG 7 cut(s) 207, 296, 332, 354, 396, 611, 939
BstDSI CCRYGG 1 cut(s) 878
BstH2I RGCGCY 1 cut(s) 496
BstHHI GCGC 1 cut(s) 495
BstKTI GATC 2 cut(s) 549, 718
BstMAI GTCTC 2 cut(s) 422, 860
BstMBI GATC 2 cut(s) 546, 715
BstMWI GCNNNNNNNGC 5 cut(s) 244, 253, 350, 475, 490
BstNI CCWGG 4 cut(s) 346, 566, 579, 842
BstSCI CCNGG 5 cut(s) 344, 420, 564, 577, 840
BstV1I GCAGC 5 cut(s) 453, 583, 605, 906, 930
Bsu15I ATCGAT 1 cut(s) 687
Bsu36I CCTNAGG 1 cut(s) 207
BsuRI GGCC 2 cut(s) 155, 368
BsuTUI ATCGAT 1 cut(s) 687
BtgI CCRYGG 1 cut(s) 878
BtsI GCAGTG 1 cut(s) 391
BtsIMutI CAGTG 1 cut(s) 391
Cac8I GCNNGC 2 cut(s) 366, 694
CfoI GCGC 1 cut(s) 495
Cfr10I RCCGGY 1 cut(s) 287
Cfr13I GGNCC 2 cut(s) 318, 444
ClaI ATCGAT 1 cut(s) 687
CseI GACGC 1 cut(s) 293
CsiI ACCWGGT 1 cut(s) 564
Csp6I GTAC 3 cut(s) 277, 340, 562
CviAII CATG 1 cut(s) 879
CviQI GTAC 3 cut(s) 277, 340, 562
DdeI CTNAG 7 cut(s) 207, 296, 332, 354, 396, 611, 939
DpnI GATC 2 cut(s) 548, 717
DpnII GATC 2 cut(s) 546, 715
Eam1104I CTCTTC 4 cut(s) 131, 254, 374, 791
EarI CTCTTC 4 cut(s) 131, 254, 374, 791
EciI GGCGGA 1 cut(s) 121
Eco130I CCWWGG 1 cut(s) 878
Eco31I GGTCTC 2 cut(s) 422, 860
Eco47I GGWCC 2 cut(s) 318, 444
Eco81I CCTNAGG 1 cut(s) 207
Eco88I CYCGRG 1 cut(s) 239
EcoRI GAATTC 1 cut(s) 212
EcoRII CCWGG 4 cut(s) 344, 564, 577, 840
EcoT14I CCWWGG 1 cut(s) 878
ErhI CCWWGG 1 cut(s) 878
FaeI CATG 1 cut(s) 882
FaiI YATR 9 cut(s) 32, 167, 591, 726, 758, 880, 887, 933, 935
FatI CATG 1 cut(s) 878
FauI CCCGC 2 cut(s) 85, 243
FauNDI CATATG 1 cut(s) 933
FblI GTMKAC 1 cut(s) 659
Fnu4HI GCNGC 7 cut(s) 257, 467, 594, 597, 856, 895, 944
Fsp4HI GCNGC 7 cut(s) 257, 467, 594, 597, 856, 895, 944
FspBI CTAG 2 cut(s) 729, 967
GlaI GCGC 1 cut(s) 494
GluI GCNGC 7 cut(s) 257, 467, 594, 597, 856, 895, 944
GsaI CCCAGC 1 cut(s) 740
GsuI CTGGAG 1 cut(s) 740
HaeII RGCGCY 1 cut(s) 496
HaeIII GGCC 2 cut(s) 155, 368
HapII CCGG 4 cut(s) 185, 260, 288, 422
HgaI GACGC 1 cut(s) 293
HhaI GCGC 1 cut(s) 495
Hin1I GRCGYC 2 cut(s) 285, 506
Hin1II CATG 1 cut(s) 882
Hin6I GCGC 1 cut(s) 493
HinP1I GCGC 1 cut(s) 493
HincII GTYRAC 1 cut(s) 660
HindII GTYRAC 1 cut(s) 660
HinfI GANTC 3 cut(s) 306, 745, 751
HpaII CCGG 4 cut(s) 185, 260, 288, 422
HphI GGTGA 3 cut(s) 5, 106, 763
Hpy166II GTNNAC 3 cut(s) 279, 633, 660
Hpy188I TCNGA 7 cut(s) 160, 178, 297, 412, 546, 744, 750
Hpy188III TCNNGA 8 cut(s) 209, 241, 310, 376, 649, 719, 865, 914
Hpy8I GTNNAC 3 cut(s) 279, 633, 660
Hpy99I CGWCG 2 cut(s) 287, 489
HpyAV CCTTC 2 cut(s) 91, 689
HpyCH4III ACNGT 1 cut(s) 871
HpyCH4IV ACGT 1 cut(s) 506
HpyCH4V TGCA 3 cut(s) 593, 599, 832
HpyF10VI GCNNNNNNNGC 5 cut(s) 244, 253, 350, 475, 490
HpyF3I CTNAG 7 cut(s) 207, 296, 332, 354, 396, 611, 939
HpySE526I ACGT 1 cut(s) 506
Hsp92I GRCGYC 2 cut(s) 285, 506
Hsp92II CATG 1 cut(s) 882
HspAI GCGC 1 cut(s) 493
KpnI GGTACC 1 cut(s) 565
Kzo9I GATC 2 cut(s) 546, 715
LguI GCTCTTC 2 cut(s) 254, 374
LmnI GCTCC 1 cut(s) 361
Lsp1109I GCAGC 5 cut(s) 453, 583, 605, 906, 930
LweI GCATC 2 cut(s) 679, 912
MabI ACCWGGT 1 cut(s) 564
MaeI CTAG 2 cut(s) 729, 967
MaeII ACGT 1 cut(s) 506
MaeIII GTNAC 1 cut(s) 779
MalI GATC 2 cut(s) 548, 717
MbiI CCGCTC 1 cut(s) 238
MboI GATC 2 cut(s) 546, 715
MboII GAAGA 7 cut(s) 118, 241, 391, 808, 823, 833, 853
MhlI GDGCHC 2 cut(s) 295, 302
MluCI AATT 3 cut(s) 179, 212, 711
MroXI GAANNNNTTC 1 cut(s) 248
MseI TTAA 2 cut(s) 627, 848
MspA1I CMGCKG 2 cut(s) 596, 943
MspI CCGG 4 cut(s) 185, 260, 288, 422
MspR9I CCNGG 5 cut(s) 346, 422, 566, 579, 842
Mva1269I GAATGC 1 cut(s) 696
MvaI CCWGG 4 cut(s) 346, 566, 579, 842
MwoI GCNNNNNNNGC 5 cut(s) 244, 253, 350, 475, 490
NciI CCSGG 1 cut(s) 422
NcoI CCATGG 1 cut(s) 878
NdeI CATATG 1 cut(s) 933
NdeII GATC 2 cut(s) 546, 715
NlaIII CATG 1 cut(s) 882
NlaIV GGNNCC 3 cut(s) 189, 319, 563
NmeAIII GCCGAG 2 cut(s) 131, 394
NmuCI GTSAC 1 cut(s) 779
NspV TTCGAA 1 cut(s) 216
PaeR7I CTCGAG 1 cut(s) 239
PciSI GCTCTTC 2 cut(s) 254, 374
PctI GAATGC 1 cut(s) 696
PdmI GAANNNNTTC 1 cut(s) 248
PfeI GAWTC 3 cut(s) 306, 745, 751
PfoI TCCNGGA 2 cut(s) 420, 840
PkrI GCNGC 7 cut(s) 258, 468, 595, 598, 857, 896, 945
Psp6I CCWGG 4 cut(s) 344, 564, 577, 840
PspFI CCCAGC 1 cut(s) 736
PspGI CCWGG 4 cut(s) 344, 564, 577, 840
PspN4I GGNNCC 3 cut(s) 189, 319, 563
PspPI GGNCC 2 cut(s) 318, 444
PvuII CAGCTG 2 cut(s) 596, 943
RsaI GTAC 3 cut(s) 278, 341, 563
RsaNI GTAC 3 cut(s) 277, 340, 562
SalI GTCGAC 1 cut(s) 658
SapI GCTCTTC 2 cut(s) 254, 374
SaqAI TTAA 2 cut(s) 627, 848
SatI GCNGC 7 cut(s) 257, 467, 594, 597, 856, 895, 944
Sau3AI GATC 2 cut(s) 546, 715
Sau96I GGNCC 2 cut(s) 318, 444
ScrFI CCNGG 5 cut(s) 346, 422, 566, 579, 842
SduI GDGCHC 2 cut(s) 295, 302
SexAI ACCWGGT 1 cut(s) 564
SfaNI GCATC 2 cut(s) 679, 912
Sfr274I CTCGAG 1 cut(s) 239
SfuI TTCGAA 1 cut(s) 216
SgrAI CRCCGGYG 1 cut(s) 287
SinI GGWCC 2 cut(s) 318, 444
SlaI CTCGAG 1 cut(s) 239
SmlI CTYRAG 1 cut(s) 239
SmoI CTYRAG 1 cut(s) 239
Sse9I AATT 3 cut(s) 179, 212, 711
SsiI CCGC 8 cut(s) 78, 90, 132, 236, 257, 787, 855, 946
SspI AATATT 1 cut(s) 325
SspMI CTAG 2 cut(s) 729, 967
StyD4I CCNGG 5 cut(s) 344, 420, 564, 577, 840
StyI CCWWGG 1 cut(s) 878
TaaI ACNGT 1 cut(s) 871
TaiI ACGT 1 cut(s) 509
TaqI TCGA 7 cut(s) 198, 216, 228, 240, 659, 687, 864
TaqII GACCGA 1 cut(s) 432
TasI AATT 3 cut(s) 179, 212, 711
TauI GCSGC 2 cut(s) 259, 858
TfiI GAWTC 3 cut(s) 306, 745, 751
Tru1I TTAA 2 cut(s) 627, 848
Tru9I TTAA 2 cut(s) 627, 848
TscAI CASTG 1 cut(s) 391
TseFI GTSAC 1 cut(s) 779
TseI GCWGC 5 cut(s) 466, 593, 596, 894, 943
Tsp45I GTSAC 1 cut(s) 779
TspGWI ACGGA 4 cut(s) 109, 502, 532, 819
TspRI CASTG 1 cut(s) 391
VpaK11BI GGWCC 2 cut(s) 318, 444
XapI RAATTY 1 cut(s) 212
XhoI CTCGAG 1 cut(s) 239
XmiI GTMKAC 1 cut(s) 659
XmnI GAANNNNTTC 1 cut(s) 248
XspI CTAG 2 cut(s) 729, 967
ZraI GACGTC 1 cut(s) 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.