MD15G1098300.v1.1

GTP-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009883
Physical Location & Seq
Forward (+)
6826044 .. 6828451
2408 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1098300.v1.1.491

Sequence Viewer

Length: 930 bp
ATGGTTCTGCTTCACCTTCCAAGAACCCACATATCTCTCTACACCCATTTCTCCCTCTTCGCAAAACCCAAAACCCCAACCTTCGCCACCGCCACAACTCTCCCCCCAGTCCTCAACTCCGCCCTCTTCTCCGCCCCCAGATCCTCCCTCTCTGCCTCCGTACCCATTTCCCAAATCCCGGAACCCCAAATCGAAACCCCCCAGGAATTCGAAACCCGCATCGAAATCCCGCTCGAGAAGCTCTTCGTGCCGCCTGAAACTGAGGTTTCGCATACCGACGCCGGTGCTCTGAGCACCCGAATCTTGAAAGGGTCCAATATTCTGCTCAGCAAGTACGCCAGGAACGCTCAGGTGGACCAGGCCGAGTTCGTGAAGAGCAGTGTGAGGACTGAGGACTGCCCCTCTGATGGTCTCCCGGAGTTTGCTCTCGTGGGTCGGTCCAATGTCGGCAAGTCCTCGCTGCTCAACTCGCTTGTGCGGCGGAAGCGGCTCGCGTTGACGTCCAAGAAACCTGGGAAAACACAATGTATAAACCATTTTCGGATCAATGGTAGCTGGTTCCTGGTGGATTTGCCCGGCCACGGGTACGCAGCTGCACCACAGGAACTTAGAACTGATTGGGTGAAGTTTACTAAAGACTACTTCCTCAAGAGGTCGACATTAGTTTCGGTTTTCCTTCTCATTGATGCCAGCATTCCTGCCAAAAAAATTGATCTGGAATACGCTAGTTGGCTGGGCCAGAATCAGCGGAAAAAGAAGAGGCATGGAGGGAAGAAAGCTGAAGAGAATGTGCAGGATTTTCAGGAGTTAATAAGCAGCTTCTTCAAGACAGTGCCTCCGTGGATTATGACGAGCAGCCTGACCAATCAGGGTCGTGATGAAATGTTACTGCATATGGCTCAGCTGCGGAACTACTGGCTGAAGCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MMR_HSR1

Protein Analysis

310

Amino Acids

34.81

Weight (kDa)

9.67

Isoelectric Point (pI)

45.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MMR_HSR1 PF01926 141 - 249 2.8e-16 50S ribosome-binding GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 503
AccBSI CCGCTC 1 cut(s) 232
AccI GTMKAC 1 cut(s) 656
AccII CGCG 1 cut(s) 494
AclWI GGATC 2 cut(s) 135, 551
AcoI YGGCCR 1 cut(s) 577
AcsI RAATTY 1 cut(s) 206
AcuI CTGAAG 1 cut(s) 801
AcyI GRCGYC 2 cut(s) 279, 500
AfaI GTAC 3 cut(s) 162, 335, 587
AfiI CCNNNNNNNGG 4 cut(s) 178, 407, 581, 582
AgsI TTSAA 2 cut(s) 307, 826
AjnI CCWGG 5 cut(s) 201, 338, 357, 511, 561
AluBI AGCT 6 cut(s) 241, 555, 593, 779, 819, 904
AluI AGCT 6 cut(s) 241, 555, 593, 779, 819, 904
Alw21I GWGCWC 2 cut(s) 289, 296
Alw26I GTCTC 1 cut(s) 416
AlwI GGATC 2 cut(s) 135, 551
Ama87I CYCGRG 1 cut(s) 233
AoxI GGCC 3 cut(s) 360, 577, 736
ApeKI GCWGC 6 cut(s) 460, 590, 593, 816, 855, 904
ApoI RAATTY 1 cut(s) 206
ArsI GACNNNNNNTTYG 2 cut(s) 649, 681
Asp700I GAANNNNTTC 1 cut(s) 242
AspS9I GGNCC 4 cut(s) 312, 355, 438, 736
AsuC2I CCSGG 3 cut(s) 179, 416, 576
AsuHPI GGTGA 2 cut(s) 5, 634
AsuII TTCGAA 1 cut(s) 210
AvaI CYCGRG 1 cut(s) 233
AvaII GGWCC 3 cut(s) 312, 355, 438
BauI CACGAG 1 cut(s) 428
Bbv12I GWGCWC 2 cut(s) 289, 296
BbvI GCAGC 6 cut(s) 447, 580, 602, 828, 867, 891
BccI CCATC 1 cut(s) 401
BcgI CGANNNNNNTGC 2 cut(s) 266, 300
BciT130I CCWGG 5 cut(s) 203, 340, 359, 513, 563
BcnI CCSGG 3 cut(s) 179, 416, 576
BcoDI GTCTC 1 cut(s) 416
BfaI CTAG 2 cut(s) 726, 928
BisI GCNGC 9 cut(s) 251, 461, 479, 488, 591, 594, 817, 856, 905
BlpI GCTNAGC 2 cut(s) 326, 900
BlsI GCNGC 9 cut(s) 252, 462, 480, 489, 592, 595, 818, 857, 906
Bme1390I CCNGG 8 cut(s) 179, 203, 340, 359, 416, 513, 563, 576
Bme18I GGWCC 3 cut(s) 312, 355, 438
BmeT110I CYCGRG 1 cut(s) 233
BmgT120I GGNCC 4 cut(s) 312, 355, 438, 736
BmiI GGNNCC 3 cut(s) 183, 313, 560
BmrFI CCNGG 8 cut(s) 179, 203, 340, 359, 416, 513, 563, 576
BmrI ACTGGG 1 cut(s) 101
BmsI GCATC 2 cut(s) 228, 676
BmuI ACTGGG 1 cut(s) 101
Bpu10I CCTNAGC 1 cut(s) 348
Bpu1102I GCTNAGC 2 cut(s) 326, 900
Bpu14I TTCGAA 1 cut(s) 210
BpuEI CTTGAG 1 cut(s) 632
BpuMI CCSGG 3 cut(s) 179, 416, 576
BsaHI GRCGYC 2 cut(s) 279, 500
BsaI GGTCTC 1 cut(s) 416
BsaJI CCNNGG 4 cut(s) 201, 512, 580, 839
BsaXI ACNNNNNCTCC 2 cut(s) 820, 850
Bsc4I CCNNNNNNNGG 4 cut(s) 178, 407, 581, 582
Bse118I RCCGGY 1 cut(s) 281
Bse1I ACTGG 2 cut(s) 107, 920
BseBI CCWGG 5 cut(s) 203, 340, 359, 513, 563
BseDI CCNNGG 4 cut(s) 201, 512, 580, 839
BseLI CCNNNNNNNGG 4 cut(s) 178, 407, 581, 582
BseMII CTCAG 6 cut(s) 252, 281, 340, 362, 381, 914
BseNI ACTGG 2 cut(s) 107, 920
BseXI GCAGC 6 cut(s) 447, 580, 602, 828, 867, 891
BseYI CCCAGC 1 cut(s) 733
BsgI GTGCAG 2 cut(s) 579, 812
Bsh1236I CGCG 1 cut(s) 494
BshFI GGCC 3 cut(s) 362, 579, 738
BsiHKAI GWGCWC 2 cut(s) 289, 296
BsiHKCI CYCGRG 1 cut(s) 233
BsiSI CCGG 4 cut(s) 179, 282, 416, 576
BslI CCNNNNNNNGG 4 cut(s) 178, 407, 581, 582
BsmAI GTCTC 1 cut(s) 416
BsmI GAATGC 1 cut(s) 693
BsnI GGCC 3 cut(s) 362, 579, 738
Bso31I GGTCTC 1 cut(s) 416
BsoBI CYCGRG 1 cut(s) 233
Bsp119I TTCGAA 1 cut(s) 210
Bsp1286I GDGCHC 2 cut(s) 289, 296
Bsp143I GATC 3 cut(s) 140, 543, 712
Bsp1720I GCTNAGC 2 cut(s) 326, 900
BspANI GGCC 3 cut(s) 362, 579, 738
BspCNI CTCAG 6 cut(s) 253, 282, 339, 361, 382, 913
BspFNI CGCG 1 cut(s) 494
BspLI GGNNCC 3 cut(s) 183, 313, 560
BspPI GGATC 2 cut(s) 135, 551
BspQI GCTCTTC 2 cut(s) 248, 368
BspT104I TTCGAA 1 cut(s) 210
BspTNI GGTCTC 1 cut(s) 416
BsrBI CCGCTC 1 cut(s) 232
BsrFI RCCGGY 1 cut(s) 281
BsrI ACTGG 2 cut(s) 107, 920
BssAI RCCGGY 1 cut(s) 281
BssECI CCNNGG 4 cut(s) 201, 512, 580, 839
BssMI GATC 3 cut(s) 140, 543, 712
BssNI GRCGYC 2 cut(s) 279, 500
BssSI CACGAG 1 cut(s) 428
Bst2BI CACGAG 1 cut(s) 428
Bst2UI CCWGG 5 cut(s) 203, 340, 359, 513, 563
Bst4CI ACNGT 1 cut(s) 832
Bst6I CTCTTC 6 cut(s) 62, 131, 248, 368, 752, 777
BstACI GRCGYC 2 cut(s) 279, 500
BstBI TTCGAA 1 cut(s) 210
BstC8I GCNNGC 2 cut(s) 492, 691
BstDEI CTNAG 7 cut(s) 261, 290, 326, 348, 390, 608, 900
BstDSI CCRYGG 2 cut(s) 580, 839
BstFNI CGCG 1 cut(s) 494
BstKTI GATC 3 cut(s) 143, 546, 715
BstMAI GTCTC 1 cut(s) 416
BstMBI GATC 3 cut(s) 140, 543, 712
BstMWI GCNNNNNNNGC 7 cut(s) 238, 247, 344, 469, 478, 484, 487
BstNI CCWGG 5 cut(s) 203, 340, 359, 513, 563
BstSCI CCNGG 8 cut(s) 177, 201, 338, 357, 414, 511, 561, 574
BstUI CGCG 1 cut(s) 494
BstV1I GCAGC 6 cut(s) 447, 580, 602, 828, 867, 891
BstX2I RGATCY 1 cut(s) 140
BstYI RGATCY 1 cut(s) 140
BsuRI GGCC 3 cut(s) 362, 579, 738
BtgI CCRYGG 2 cut(s) 580, 839
BtsI GCAGTG 1 cut(s) 385
BtsIMutI CAGTG 2 cut(s) 385, 837
Cac8I GCNNGC 2 cut(s) 492, 691
Cfr10I RCCGGY 1 cut(s) 281
Cfr13I GGNCC 4 cut(s) 312, 355, 438, 736
CseI GACGC 1 cut(s) 287
Csp6I GTAC 3 cut(s) 161, 334, 586
CviAII CATG 1 cut(s) 764
CviQI GTAC 3 cut(s) 161, 334, 586
DdeI CTNAG 7 cut(s) 261, 290, 326, 348, 390, 608, 900
DpnI GATC 3 cut(s) 142, 545, 714
DpnII GATC 3 cut(s) 140, 543, 712
EaeI YGGCCR 1 cut(s) 577
Eam1104I CTCTTC 6 cut(s) 62, 131, 248, 368, 752, 777
EarI CTCTTC 6 cut(s) 62, 131, 248, 368, 752, 777
EciI GGCGGA 3 cut(s) 109, 121, 496
Eco31I GGTCTC 1 cut(s) 416
Eco47I GGWCC 3 cut(s) 312, 355, 438
Eco57I CTGAAG 1 cut(s) 801
Eco88I CYCGRG 1 cut(s) 233
EcoRI GAATTC 1 cut(s) 206
EcoRII CCWGG 5 cut(s) 201, 338, 357, 511, 561
FaeI CATG 1 cut(s) 767
FaiI YATR 7 cut(s) 32, 273, 530, 765, 848, 894, 896
FatI CATG 1 cut(s) 763
FauI CCCGC 2 cut(s) 224, 237
FauNDI CATATG 1 cut(s) 894
FblI GTMKAC 1 cut(s) 656
Fnu4HI GCNGC 9 cut(s) 251, 461, 479, 488, 591, 594, 817, 856, 905
Fsp4HI GCNGC 9 cut(s) 251, 461, 479, 488, 591, 594, 817, 856, 905
FspBI CTAG 2 cut(s) 726, 928
GluI GCNGC 9 cut(s) 251, 461, 479, 488, 591, 594, 817, 856, 905
GsaI CCCAGC 1 cut(s) 737
HaeIII GGCC 3 cut(s) 362, 579, 738
HapII CCGG 4 cut(s) 179, 282, 416, 576
HgaI GACGC 1 cut(s) 287
Hin1I GRCGYC 2 cut(s) 279, 500
Hin1II CATG 1 cut(s) 767
HincII GTYRAC 2 cut(s) 498, 657
HindII GTYRAC 2 cut(s) 498, 657
HinfI GANTC 2 cut(s) 300, 742
HpaII CCGG 4 cut(s) 179, 282, 416, 576
HphI GGTGA 2 cut(s) 5, 634
Hpy166II GTNNAC 4 cut(s) 355, 498, 630, 657
Hpy188I TCNGA 3 cut(s) 291, 406, 543
Hpy188III TCNNGA 8 cut(s) 235, 304, 370, 649, 716, 803, 826, 875
Hpy8I GTNNAC 4 cut(s) 355, 498, 630, 657
Hpy99I CGWCG 1 cut(s) 281
HpyAV CCTTC 3 cut(s) 26, 91, 686
HpyCH4III ACNGT 1 cut(s) 832
HpyCH4IV ACGT 1 cut(s) 500
HpyCH4V TGCA 3 cut(s) 596, 793, 892
HpyF10VI GCNNNNNNNGC 7 cut(s) 238, 247, 344, 469, 478, 484, 487
HpyF3I CTNAG 7 cut(s) 261, 290, 326, 348, 390, 608, 900
HpySE526I ACGT 1 cut(s) 500
Hsp92I GRCGYC 2 cut(s) 279, 500
Hsp92II CATG 1 cut(s) 767
Kzo9I GATC 3 cut(s) 140, 543, 712
LguI GCTCTTC 2 cut(s) 248, 368
Lsp1109I GCAGC 6 cut(s) 447, 580, 602, 828, 867, 891
LweI GCATC 2 cut(s) 228, 676
MaeI CTAG 2 cut(s) 726, 928
MaeII ACGT 1 cut(s) 500
MaeIII GTNAC 1 cut(s) 885
MalI GATC 3 cut(s) 142, 545, 714
MbiI CCGCTC 1 cut(s) 232
MboI GATC 3 cut(s) 140, 543, 712
MboII GAAGA 8 cut(s) 49, 118, 235, 385, 769, 784, 794, 814
MflI RGATCY 1 cut(s) 140
MhlI GDGCHC 2 cut(s) 289, 296
MluCI AATT 2 cut(s) 206, 708
MroXI GAANNNNTTC 1 cut(s) 242
MseI TTAA 1 cut(s) 809
MspA1I CMGCKG 3 cut(s) 593, 748, 904
MspI CCGG 4 cut(s) 179, 282, 416, 576
MspR9I CCNGG 8 cut(s) 179, 203, 340, 359, 416, 513, 563, 576
Mva1269I GAATGC 1 cut(s) 693
MvaI CCWGG 5 cut(s) 203, 340, 359, 513, 563
MvnI CGCG 1 cut(s) 494
MwoI GCNNNNNNNGC 7 cut(s) 238, 247, 344, 469, 478, 484, 487
NciI CCSGG 3 cut(s) 179, 416, 576
NdeI CATATG 1 cut(s) 894
NdeII GATC 3 cut(s) 140, 543, 712
NlaIII CATG 1 cut(s) 767
NlaIV GGNNCC 3 cut(s) 183, 313, 560
NmeAIII GCCGAG 1 cut(s) 388
NspV TTCGAA 1 cut(s) 210
PaeR7I CTCGAG 1 cut(s) 233
PciSI GCTCTTC 2 cut(s) 248, 368
PctI GAATGC 1 cut(s) 693
PdmI GAANNNNTTC 1 cut(s) 242
PfeI GAWTC 2 cut(s) 300, 742
PfoI TCCNGGA 2 cut(s) 177, 414
PkrI GCNGC 9 cut(s) 252, 462, 480, 489, 592, 595, 818, 857, 906
Psp6I CCWGG 5 cut(s) 201, 338, 357, 511, 561
PspFI CCCAGC 1 cut(s) 733
PspGI CCWGG 5 cut(s) 201, 338, 357, 511, 561
PspN4I GGNNCC 3 cut(s) 183, 313, 560
PspPI GGNCC 4 cut(s) 312, 355, 438, 736
PsuI RGATCY 1 cut(s) 140
PvuII CAGCTG 2 cut(s) 593, 904
RsaI GTAC 3 cut(s) 162, 335, 587
RsaNI GTAC 3 cut(s) 161, 334, 586
SalI GTCGAC 1 cut(s) 655
SapI GCTCTTC 2 cut(s) 248, 368
SaqAI TTAA 1 cut(s) 809
SatI GCNGC 9 cut(s) 251, 461, 479, 488, 591, 594, 817, 856, 905
Sau3AI GATC 3 cut(s) 140, 543, 712
Sau96I GGNCC 4 cut(s) 312, 355, 438, 736
ScrFI CCNGG 8 cut(s) 179, 203, 340, 359, 416, 513, 563, 576
SduI GDGCHC 2 cut(s) 289, 296
SfaNI GCATC 2 cut(s) 228, 676
Sfr274I CTCGAG 1 cut(s) 233
SfuI TTCGAA 1 cut(s) 210
SgrAI CRCCGGYG 1 cut(s) 281
SinI GGWCC 3 cut(s) 312, 355, 438
SlaI CTCGAG 1 cut(s) 233
SmlI CTYRAG 2 cut(s) 233, 647
SmoI CTYRAG 2 cut(s) 233, 647
Sse9I AATT 2 cut(s) 206, 708
SspI AATATT 1 cut(s) 319
SspMI CTAG 2 cut(s) 726, 928
StyD4I CCNGG 8 cut(s) 177, 201, 338, 357, 414, 511, 561, 574
TaaI ACNGT 1 cut(s) 832
TaiI ACGT 1 cut(s) 503
TaqI TCGA 5 cut(s) 192, 210, 222, 234, 656
TaqII GACCGA 1 cut(s) 426
TasI AATT 2 cut(s) 206, 708
TauI GCSGC 3 cut(s) 253, 481, 490
TfiI GAWTC 2 cut(s) 300, 742
Tru1I TTAA 1 cut(s) 809
Tru9I TTAA 1 cut(s) 809
TscAI CASTG 2 cut(s) 385, 837
TseI GCWGC 6 cut(s) 460, 590, 593, 816, 855, 904
TspDTI ATGAA 1 cut(s) 894
TspGWI ACGGA 2 cut(s) 148, 828
TspRI CASTG 2 cut(s) 385, 837
VpaK11BI GGWCC 3 cut(s) 312, 355, 438
XapI RAATTY 1 cut(s) 206
XhoI CTCGAG 1 cut(s) 233
XmiI GTMKAC 1 cut(s) 656
XmnI GAANNNNTTC 1 cut(s) 242
XspI CTAG 2 cut(s) 726, 928
ZraI GACGTC 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.