pycom08g09940

GTP-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0009883
Physical Location & Seq
Forward (+)
8230617 .. 8232508
1892 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g09940.4

Sequence Viewer

Length: 972 bp
ATGGTTCTGCTTCACCTCCCAAGAACCCACATATCCCTCTCCACCCATTTCTCTCTTTTCCCGAAACCCAAAACCCCCGCCTTCGCCACCGCCACAACTCTCCCCCCAATCCTCACCTCCGCCCTCTTCTCCGCCCCCCAGTCCACCCTCTCAGCCTCCGAACCCATACCCATTTCCGAATTGCCGGAACCCCAAATCGAACCCCCTCAGGAATTCGAAACCCACATCGAAATCCCGCTCGAGAAGCTCTTCGTGCCGCCAGAAACCGAGGTCTCGTACACCGACGCCGGTGCTCTGAGCACCCGAATCTTGAAGGGGTCCAATATTCTGCTCAGTAAGTACGCCAGGAACGCTCAGGTGGAGCAGGCCGAGTTCGTGAAGAGCAGTGTGAGGACTGAGGATTGCCCCTCTGATAGTCTCCCGGAGTTTGCTCTCGTGGGTCGGTCCAATGTCGGCAAGTCCTCGCTGCTCAACTCGCTCGTGCGACGGAAGCGCCTCGCTTTGACGTCCAAGAAACCTGGGAAAACACAATGTATCAATCATTTTCGGATCAATGATAGCTGGTACCTGGTGGATTTGCCAGGCTACGGGTATGCAGCCGCACCACAGGAACTTAGAACTGACTGGGTTAAGTTTACTAAAGACTACTTCCTGAATAGGTCGACATTAGTTTCGGTTTTCCTTCTCATCGATGCCAGCATTCCTGCCAAAAAAATTGATCTGGAGTATGCTAGTTGGCTGGGTCAGAATCAGATTCCTATGACTTTGATATTCACCAAATGTGACAAGCGGAAAAAGAAGAGGCACGGAGGGAAGAAAGCAGAAGAAAATGTGCAGGATTTCCAGGAGTTAATAAGTGGCTTCTTCGAGACCGTGCCTCCGTGGATTATGACCAGCAGCCTGACCAGTCAGGGTCGTGATGAGATGCTATTACATATGGCTCAGCTGCGGAACTACTGGCTGAAGCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MMR_HSR1

Protein Analysis

324

Amino Acids

36.43

Weight (kDa)

8.96

Isoelectric Point (pI)

52.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 509
Acc65I GGTACC 1 cut(s) 564
AccB1I GGYRCC 1 cut(s) 564
AccBSI CCGCTC 1 cut(s) 238
AccI GTMKAC 1 cut(s) 662
AciI CCGC 9 cut(s) 78, 90, 120, 132, 236, 257, 600, 790, 949
AclWI GGATC 1 cut(s) 557
AcsI RAATTY 1 cut(s) 212
AcyI GRCGYC 2 cut(s) 285, 506
AfaI GTAC 3 cut(s) 278, 341, 566
AfiI CCNNNNNNNGG 1 cut(s) 587
AgsI TTSAA 1 cut(s) 313
AjnI CCWGG 5 cut(s) 344, 517, 567, 580, 843
AluBI AGCT 3 cut(s) 247, 561, 946
AluI AGCT 3 cut(s) 247, 561, 946
Alw21I GWGCWC 2 cut(s) 295, 302
Alw26I GTCTC 3 cut(s) 277, 422, 863
AlwI GGATC 1 cut(s) 557
Ama87I CYCGRG 1 cut(s) 239
AoxI GGCC 1 cut(s) 366
ApeKI GCWGC 4 cut(s) 466, 596, 897, 946
ApoI RAATTY 1 cut(s) 212
ArsI GACNNNNNNTTYG 2 cut(s) 655, 687
Asp700I GAANNNNTTC 1 cut(s) 248
Asp718I GGTACC 1 cut(s) 564
AspLEI GCGC 1 cut(s) 495
AspS9I GGNCC 2 cut(s) 318, 444
AsuC2I CCSGG 1 cut(s) 422
AsuHPI GGTGA 3 cut(s) 5, 106, 766
AsuII TTCGAA 1 cut(s) 216
AvaI CYCGRG 1 cut(s) 239
AvaII GGWCC 2 cut(s) 318, 444
AxyI CCTNAGG 1 cut(s) 207
BanI GGYRCC 1 cut(s) 564
BauI CACGAG 2 cut(s) 434, 479
Bbv12I GWGCWC 2 cut(s) 295, 302
BbvI GCAGC 4 cut(s) 453, 608, 909, 933
BcgI CGANNNNNNTGC 2 cut(s) 272, 306
BciT130I CCWGG 5 cut(s) 346, 519, 569, 582, 845
BcnI CCSGG 1 cut(s) 422
BcoDI GTCTC 3 cut(s) 277, 422, 863
BfaI CTAG 2 cut(s) 732, 970
BfoI RGCGCY 1 cut(s) 496
BisI GCNGC 6 cut(s) 257, 467, 597, 600, 898, 947
BlpI GCTNAGC 1 cut(s) 942
BlsI GCNGC 6 cut(s) 258, 468, 598, 601, 899, 948
Bme1390I CCNGG 6 cut(s) 346, 422, 519, 569, 582, 845
Bme18I GGWCC 2 cut(s) 318, 444
BmeT110I CYCGRG 1 cut(s) 239
BmgT120I GGNCC 2 cut(s) 318, 444
BmiI GGNNCC 3 cut(s) 189, 319, 566
BmrFI CCNGG 6 cut(s) 346, 422, 519, 569, 582, 845
BmrI ACTGGG 2 cut(s) 133, 634
BmsI GCATC 2 cut(s) 682, 915
BmuI ACTGGG 2 cut(s) 133, 634
BpmI CTGGAG 1 cut(s) 743
Bpu10I CCTNAGC 1 cut(s) 354
Bpu1102I GCTNAGC 1 cut(s) 942
Bpu14I TTCGAA 1 cut(s) 216
BpuMI CCSGG 1 cut(s) 422
Bsa29I ATCGAT 1 cut(s) 690
BsaHI GRCGYC 2 cut(s) 285, 506
BsaI GGTCTC 2 cut(s) 277, 863
BsaJI CCNNGG 3 cut(s) 267, 518, 881
BsaXI ACNNNNNCTCC 2 cut(s) 862, 892
Bsc4I CCNNNNNNNGG 1 cut(s) 587
Bse118I RCCGGY 1 cut(s) 287
Bse1I ACTGG 4 cut(s) 139, 629, 906, 962
Bse21I CCTNAGG 1 cut(s) 207
BseBI CCWGG 5 cut(s) 346, 519, 569, 582, 845
BseCI ATCGAT 1 cut(s) 690
BseDI CCNNGG 3 cut(s) 267, 518, 881
BseLI CCNNNNNNNGG 1 cut(s) 587
BseMII CTCAG 7 cut(s) 165, 221, 287, 346, 368, 387, 956
BseNI ACTGG 4 cut(s) 139, 629, 906, 962
BseXI GCAGC 4 cut(s) 453, 608, 909, 933
BseYI CCCAGC 1 cut(s) 739
BsgI GTGCAG 1 cut(s) 854
BshFI GGCC 1 cut(s) 368
BshNI GGYRCC 1 cut(s) 564
BshVI ATCGAT 1 cut(s) 690
BsiHKAI GWGCWC 2 cut(s) 295, 302
BsiHKCI CYCGRG 1 cut(s) 239
BsiSI CCGG 3 cut(s) 185, 288, 422
BslI CCNNNNNNNGG 1 cut(s) 587
BsmAI GTCTC 3 cut(s) 277, 422, 863
BsmI GAATGC 1 cut(s) 699
BsnI GGCC 1 cut(s) 368
Bso31I GGTCTC 2 cut(s) 277, 863
BsoBI CYCGRG 1 cut(s) 239
Bsp119I TTCGAA 1 cut(s) 216
Bsp1286I GDGCHC 2 cut(s) 295, 302
Bsp143I GATC 2 cut(s) 549, 718
Bsp1720I GCTNAGC 1 cut(s) 942
BspACI CCGC 9 cut(s) 78, 90, 120, 132, 236, 257, 600, 790, 949
BspANI GGCC 1 cut(s) 368
BspCNI CTCAG 7 cut(s) 164, 220, 288, 345, 367, 388, 955
BspDI ATCGAT 1 cut(s) 690
BspLI GGNNCC 3 cut(s) 189, 319, 566
BspPI GGATC 1 cut(s) 557
BspQI GCTCTTC 2 cut(s) 254, 374
BspT104I TTCGAA 1 cut(s) 216
BspT107I GGYRCC 1 cut(s) 564
BspTNI GGTCTC 2 cut(s) 277, 863
BsrBI CCGCTC 1 cut(s) 238
BsrFI RCCGGY 1 cut(s) 287
BsrI ACTGG 4 cut(s) 139, 629, 906, 962
BssAI RCCGGY 1 cut(s) 287
BssECI CCNNGG 3 cut(s) 267, 518, 881
BssMI GATC 2 cut(s) 549, 718
BssNI GRCGYC 2 cut(s) 285, 506
BssSI CACGAG 2 cut(s) 434, 479
Bst2BI CACGAG 2 cut(s) 434, 479
Bst2UI CCWGG 5 cut(s) 346, 519, 569, 582, 845
Bst4CI ACNGT 1 cut(s) 874
Bst6I CTCTTC 4 cut(s) 131, 254, 374, 794
BstACI GRCGYC 2 cut(s) 285, 506
BstBI TTCGAA 1 cut(s) 216
BstC8I GCNNGC 2 cut(s) 366, 697
BstDEI CTNAG 8 cut(s) 151, 207, 296, 332, 354, 396, 614, 942
BstDSI CCRYGG 1 cut(s) 881
BstH2I RGCGCY 1 cut(s) 496
BstHHI GCGC 1 cut(s) 495
BstKTI GATC 2 cut(s) 552, 721
BstMAI GTCTC 3 cut(s) 277, 422, 863
BstMBI GATC 2 cut(s) 549, 718
BstMWI GCNNNNNNNGC 5 cut(s) 244, 253, 350, 475, 490
BstNI CCWGG 5 cut(s) 346, 519, 569, 582, 845
BstSCI CCNGG 6 cut(s) 344, 420, 517, 567, 580, 843
BstV1I GCAGC 4 cut(s) 453, 608, 909, 933
Bsu15I ATCGAT 1 cut(s) 690
Bsu36I CCTNAGG 1 cut(s) 207
BsuRI GGCC 1 cut(s) 368
BsuTUI ATCGAT 1 cut(s) 690
BtgI CCRYGG 1 cut(s) 881
BtsI GCAGTG 1 cut(s) 391
BtsIMutI CAGTG 1 cut(s) 391
Cac8I GCNNGC 2 cut(s) 366, 697
CfoI GCGC 1 cut(s) 495
Cfr10I RCCGGY 1 cut(s) 287
Cfr13I GGNCC 2 cut(s) 318, 444
ClaI ATCGAT 1 cut(s) 690
CseI GACGC 1 cut(s) 293
CsiI ACCWGGT 1 cut(s) 567
Csp6I GTAC 3 cut(s) 277, 340, 565
CviQI GTAC 3 cut(s) 277, 340, 565
DdeI CTNAG 8 cut(s) 151, 207, 296, 332, 354, 396, 614, 942
DpnI GATC 2 cut(s) 551, 720
DpnII GATC 2 cut(s) 549, 718
Eam1104I CTCTTC 4 cut(s) 131, 254, 374, 794
EarI CTCTTC 4 cut(s) 131, 254, 374, 794
EciI GGCGGA 2 cut(s) 109, 121
Eco31I GGTCTC 2 cut(s) 277, 863
Eco47I GGWCC 2 cut(s) 318, 444
Eco81I CCTNAGG 1 cut(s) 207
Eco88I CYCGRG 1 cut(s) 239
EcoRI GAATTC 1 cut(s) 212
EcoRII CCWGG 5 cut(s) 344, 517, 567, 580, 843
FaiI YATR 8 cut(s) 32, 167, 594, 729, 761, 890, 936, 938
FauI CCCGC 2 cut(s) 85, 243
FauNDI CATATG 1 cut(s) 936
FblI GTMKAC 1 cut(s) 662
Fnu4HI GCNGC 6 cut(s) 257, 467, 597, 600, 898, 947
Fsp4HI GCNGC 6 cut(s) 257, 467, 597, 600, 898, 947
FspBI CTAG 2 cut(s) 732, 970
GlaI GCGC 1 cut(s) 494
GluI GCNGC 6 cut(s) 257, 467, 597, 600, 898, 947
GsaI CCCAGC 1 cut(s) 743
GsuI CTGGAG 1 cut(s) 743
HaeII RGCGCY 1 cut(s) 496
HaeIII GGCC 1 cut(s) 368
HapII CCGG 3 cut(s) 185, 288, 422
HgaI GACGC 1 cut(s) 293
HhaI GCGC 1 cut(s) 495
Hin1I GRCGYC 2 cut(s) 285, 506
Hin6I GCGC 1 cut(s) 493
HinP1I GCGC 1 cut(s) 493
HincII GTYRAC 1 cut(s) 663
HindII GTYRAC 1 cut(s) 663
HinfI GANTC 3 cut(s) 306, 748, 754
HpaII CCGG 3 cut(s) 185, 288, 422
HphI GGTGA 3 cut(s) 5, 106, 766
Hpy166II GTNNAC 4 cut(s) 144, 279, 636, 663
Hpy188I TCNGA 7 cut(s) 160, 178, 297, 412, 549, 747, 753
Hpy188III TCNNGA 9 cut(s) 61, 209, 241, 310, 376, 652, 722, 868, 917
Hpy8I GTNNAC 4 cut(s) 144, 279, 636, 663
Hpy99I CGWCG 2 cut(s) 287, 489
HpyAV CCTTC 3 cut(s) 91, 307, 692
HpyCH4III ACNGT 1 cut(s) 874
HpyCH4IV ACGT 1 cut(s) 506
HpyCH4V TGCA 2 cut(s) 596, 835
HpyF10VI GCNNNNNNNGC 5 cut(s) 244, 253, 350, 475, 490
HpyF3I CTNAG 8 cut(s) 151, 207, 296, 332, 354, 396, 614, 942
HpySE526I ACGT 1 cut(s) 506
Hsp92I GRCGYC 2 cut(s) 285, 506
HspAI GCGC 1 cut(s) 493
KpnI GGTACC 1 cut(s) 568
Kzo9I GATC 2 cut(s) 549, 718
LguI GCTCTTC 2 cut(s) 254, 374
LmnI GCTCC 1 cut(s) 361
Lsp1109I GCAGC 4 cut(s) 453, 608, 909, 933
LweI GCATC 2 cut(s) 682, 915
MabI ACCWGGT 1 cut(s) 567
MaeI CTAG 2 cut(s) 732, 970
MaeII ACGT 1 cut(s) 506
MaeIII GTNAC 1 cut(s) 782
MalI GATC 2 cut(s) 551, 720
MbiI CCGCTC 1 cut(s) 238
MboI GATC 2 cut(s) 549, 718
MboII GAAGA 7 cut(s) 118, 241, 391, 811, 826, 836, 856
MhlI GDGCHC 2 cut(s) 295, 302
MluCI AATT 3 cut(s) 179, 212, 714
MroXI GAANNNNTTC 1 cut(s) 248
MseI TTAA 2 cut(s) 630, 851
MspA1I CMGCKG 1 cut(s) 946
MspI CCGG 3 cut(s) 185, 288, 422
MspR9I CCNGG 6 cut(s) 346, 422, 519, 569, 582, 845
Mva1269I GAATGC 1 cut(s) 699
MvaI CCWGG 5 cut(s) 346, 519, 569, 582, 845
MwoI GCNNNNNNNGC 5 cut(s) 244, 253, 350, 475, 490
NciI CCSGG 1 cut(s) 422
NdeI CATATG 1 cut(s) 936
NdeII GATC 2 cut(s) 549, 718
NlaIV GGNNCC 3 cut(s) 189, 319, 566
NmeAIII GCCGAG 1 cut(s) 394
NmuCI GTSAC 1 cut(s) 782
NspV TTCGAA 1 cut(s) 216
PaeR7I CTCGAG 1 cut(s) 239
PciSI GCTCTTC 2 cut(s) 254, 374
PctI GAATGC 1 cut(s) 699
PdmI GAANNNNTTC 1 cut(s) 248
PfeI GAWTC 3 cut(s) 306, 748, 754
PfoI TCCNGGA 2 cut(s) 420, 843
PkrI GCNGC 6 cut(s) 258, 468, 598, 601, 899, 948
Psp6I CCWGG 5 cut(s) 344, 517, 567, 580, 843
PspFI CCCAGC 1 cut(s) 739
PspGI CCWGG 5 cut(s) 344, 517, 567, 580, 843
PspN4I GGNNCC 3 cut(s) 189, 319, 566
PspPI GGNCC 2 cut(s) 318, 444
PvuII CAGCTG 1 cut(s) 946
RsaI GTAC 3 cut(s) 278, 341, 566
RsaNI GTAC 3 cut(s) 277, 340, 565
SalI GTCGAC 1 cut(s) 661
SapI GCTCTTC 2 cut(s) 254, 374
SaqAI TTAA 2 cut(s) 630, 851
SatI GCNGC 6 cut(s) 257, 467, 597, 600, 898, 947
Sau3AI GATC 2 cut(s) 549, 718
Sau96I GGNCC 2 cut(s) 318, 444
ScrFI CCNGG 6 cut(s) 346, 422, 519, 569, 582, 845
SduI GDGCHC 2 cut(s) 295, 302
SexAI ACCWGGT 1 cut(s) 567
SfaNI GCATC 2 cut(s) 682, 915
Sfr274I CTCGAG 1 cut(s) 239
SfuI TTCGAA 1 cut(s) 216
SgrAI CRCCGGYG 1 cut(s) 287
SinI GGWCC 2 cut(s) 318, 444
SlaI CTCGAG 1 cut(s) 239
SmlI CTYRAG 1 cut(s) 239
SmoI CTYRAG 1 cut(s) 239
Sse9I AATT 3 cut(s) 179, 212, 714
SsiI CCGC 9 cut(s) 78, 90, 120, 132, 236, 257, 600, 790, 949
SspI AATATT 1 cut(s) 325
SspMI CTAG 2 cut(s) 732, 970
StyD4I CCNGG 6 cut(s) 344, 420, 517, 567, 580, 843
TaaI ACNGT 1 cut(s) 874
TaiI ACGT 1 cut(s) 509
TaqI TCGA 7 cut(s) 198, 216, 228, 240, 662, 690, 867
TaqII GACCGA 1 cut(s) 432
TasI AATT 3 cut(s) 179, 212, 714
TauI GCSGC 2 cut(s) 259, 602
TfiI GAWTC 3 cut(s) 306, 748, 754
Tru1I TTAA 2 cut(s) 630, 851
Tru9I TTAA 2 cut(s) 630, 851
TscAI CASTG 1 cut(s) 391
TseFI GTSAC 1 cut(s) 782
TseI GCWGC 4 cut(s) 466, 596, 897, 946
Tsp45I GTSAC 1 cut(s) 782
TspGWI ACGGA 3 cut(s) 502, 822, 870
TspRI CASTG 1 cut(s) 391
VpaK11BI GGWCC 2 cut(s) 318, 444
XapI RAATTY 1 cut(s) 212
XhoI CTCGAG 1 cut(s) 239
XmiI GTMKAC 1 cut(s) 662
XmnI GAANNNNTTC 1 cut(s) 248
XspI CTAG 2 cut(s) 732, 970
ZraI GACGTC 1 cut(s) 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.