MD08G1165700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0013484
Physical Location & Seq
Reverse (-)
19505730 .. 19507968
2239 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1165700.v1.1.491

Sequence Viewer

Length: 795 bp
ATGGGAGGACATGGAGCTATAGAGGTGGCGAAGACGGTGATGGAGGTTGCTGATGTGGCGTGGACCGCCATGGAACACGGCCGCCATCACCTCCACCACGAGGACCACGACGGTCAGGGAACGGTTTCCGACGAAGAACTGGAGGCTCTGCGGGCGGAGAATCGGCGGCTGAGGAACTCGCTGGAGCAGAATCTGAAGCTGCTCGAGAACCTCTCCGAGTCGCCTGCGGTTTTGGACGATTGCCCTCCTGATCTGTATGCTCGGTTAGTGGCGACGGTGGATTCGAACAGCTTCTTGACTAGAATCAAAGCTCTTCAGAAGGAATCCATTGATGATTATCAGTTTCCTTTCAAACCAGCCACAGGAAGTGATTTGGAGAAAGCTGAAGTTCAAATCAAAATTGACAATGAAGAAGCCAGTTGGTGGGTGTGGATCACGGATGATATGATTCCGGGTAAAGTCGAGGAGAGGAGTGGGATTGACAATGAGAGCTATGTTGTTGTTAGTGAGGAGCATGTGGTGGATGGGATTGCCAATTTTATGGCCAAGTGTATTGTGTCAAACCCGAAAGCTCGCAACTTGACGCCAGAGGAGCTGCAGAAAATTGTAGCTAAAGCAATGGGTGGTGTCGGCAAATTGGAGAAAGTATTGGGCATTTGGCATGCAGGGAAGTTGTTCTATACTTTGTCCACTTGGGGACTTGCTCTAACTGGATTGTATAGGAGCCGTGCGGTATTAAAATTTGCGGCGATGGGGCTGCACACAACTAGCAAAGCAATTATGAGAGCAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

265

Amino Acids

29.35

Weight (kDa)

5.4

Isoelectric Point (pI)

30.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44770 AT1G44770
fragaria_vesca FvH4_2g26730
malus_domestica MD08G1165700.v1.1 MD15G1351500.v1.1
pyrus_communis pycom12654g00110 pycom15g31390
rosa_chinensis RchiOBHm_Chr6g0295611
rosa_laevigata RLG00000011785
rosa_multiflora Rmu_sc0013988.1_g000004
rosa_roxburghii Rroxscaffold_7G00172320
rosa_rugosa Rorug06G0252700 Rorug06G0252800
rosa_samantha Rh6AG365800 Rh6BG373900 Rh6CG380200 Rh6DG366800
rosa_wichuraiana Rw6G031920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 423
AciI CCGC 8 cut(s) 66, 82, 151, 155, 166, 227, 731, 746
AclWI GGATC 1 cut(s) 440
AcoI YGGCCR 2 cut(s) 79, 543
AcsI RAATTY 1 cut(s) 740
AcuI CTGAAG 3 cut(s) 215, 299, 405
AcyI GRCGYC 1 cut(s) 584
AfiI CCNNNNNNNGG 3 cut(s) 100, 362, 423
AgsI TTSAA 2 cut(s) 352, 392
AluBI AGCT 9 cut(s) 17, 199, 291, 311, 383, 492, 572, 595, 611
AluI AGCT 9 cut(s) 17, 199, 291, 311, 383, 492, 572, 595, 611
AlwI GGATC 1 cut(s) 440
AlwNI CAGNNNCTG 1 cut(s) 193
Ama87I CYCGRG 1 cut(s) 203
AoxI GGCC 2 cut(s) 79, 543
ApeKI GCWGC 3 cut(s) 199, 595, 757
ApoI RAATTY 1 cut(s) 740
ArsI GACNNNNNNTTYG 2 cut(s) 265, 297
Asp700I GAANNNNTTC 3 cut(s) 124, 290, 674
AspS9I GGNCC 2 cut(s) 63, 103
AsuC2I CCSGG 1 cut(s) 453
AsuHPI GGTGA 2 cut(s) 49, 80
AsuII TTCGAA 1 cut(s) 284
AvaI CYCGRG 1 cut(s) 203
AvaII GGWCC 2 cut(s) 63, 103
BalI TGGCCA 1 cut(s) 545
BauI CACGAG 1 cut(s) 98
BbsI GAAGAC 1 cut(s) 38
BbvCI CCTCAGC 1 cut(s) 170
BbvI GCAGC 3 cut(s) 186, 582, 744
BccI CCATC 4 cut(s) 34, 93, 518, 745
BceAI ACGGC 2 cut(s) 94, 711
BcgI CGANNNNNNTGC 4 cut(s) 206, 240, 739, 773
BcnI CCSGG 1 cut(s) 453
BfaI CTAG 2 cut(s) 300, 768
BfmI CTRYAG 2 cut(s) 18, 596
BisI GCNGC 6 cut(s) 82, 167, 200, 596, 747, 758
BlsI GCNGC 6 cut(s) 83, 168, 201, 597, 748, 759
Bme1390I CCNGG 1 cut(s) 453
Bme18I GGWCC 2 cut(s) 63, 103
BmeT110I CYCGRG 1 cut(s) 203
BmgT120I GGNCC 2 cut(s) 63, 103
BmiI GGNNCC 1 cut(s) 725
BmrFI CCNGG 1 cut(s) 453
BpiI GAAGAC 1 cut(s) 38
BplI GAGNNNNNCTC 2 cut(s) 197, 229
BpmI CTGGAG 2 cut(s) 161, 203
Bpu10I CCTNAGC 1 cut(s) 170
Bpu14I TTCGAA 1 cut(s) 284
BpuMI CCSGG 1 cut(s) 453
BsaBI GATNNNNATC 1 cut(s) 336
BsaHI GRCGYC 1 cut(s) 584
BsaJI CCNNGG 1 cut(s) 69
Bsc4I CCNNNNNNNGG 3 cut(s) 100, 362, 423
Bse1I ACTGG 3 cut(s) 144, 417, 715
Bse3DI GCAATG 2 cut(s) 624, 795
Bse8I GATNNNNATC 1 cut(s) 336
BseDI CCNNGG 1 cut(s) 69
BseGI GGATG 2 cut(s) 445, 529
BseJI GATNNNNATC 1 cut(s) 336
BseLI CCNNNNNNNGG 3 cut(s) 100, 362, 423
BseMI GCAATG 2 cut(s) 624, 795
BseMII CTCAG 1 cut(s) 161
BseNI ACTGG 3 cut(s) 144, 417, 715
BseRI GAGGAG 4 cut(s) 479, 484, 524, 605
BseX3I CGGCCG 1 cut(s) 79
BseXI GCAGC 3 cut(s) 186, 582, 744
BsgI GTGCAG 1 cut(s) 743
Bsh1285I CGRYCG 1 cut(s) 82
BshFI GGCC 2 cut(s) 81, 545
BsiEI CGRYCG 1 cut(s) 82
BsiHKCI CYCGRG 1 cut(s) 203
BsiSI CCGG 1 cut(s) 452
BslFI GGGAC 1 cut(s) 711
BslI CCNNNNNNNGG 3 cut(s) 100, 362, 423
BsmFI GGGAC 1 cut(s) 711
BsnI GGCC 2 cut(s) 81, 545
BsoBI CYCGRG 1 cut(s) 203
Bsp119I TTCGAA 1 cut(s) 284
Bsp143I GATC 2 cut(s) 250, 432
Bsp19I CCATGG 1 cut(s) 69
BspACI CCGC 8 cut(s) 66, 82, 151, 155, 166, 227, 731, 746
BspANI GGCC 2 cut(s) 81, 545
BspCNI CTCAG 1 cut(s) 162
BspLI GGNNCC 1 cut(s) 725
BspMAI CTGCAG 1 cut(s) 600
BspPI GGATC 1 cut(s) 440
BspQI GCTCTTC 1 cut(s) 318
BspT104I TTCGAA 1 cut(s) 284
BsrDI GCAATG 2 cut(s) 624, 795
BsrI ACTGG 3 cut(s) 144, 417, 715
BssECI CCNNGG 1 cut(s) 69
BssMI GATC 2 cut(s) 250, 432
BssNI GRCGYC 1 cut(s) 584
BssSI CACGAG 1 cut(s) 98
BssT1I CCWWGG 1 cut(s) 69
Bst2BI CACGAG 1 cut(s) 98
Bst4CI ACNGT 4 cut(s) 37, 113, 124, 277
Bst6I CTCTTC 1 cut(s) 318
BstACI GRCGYC 1 cut(s) 584
BstBI TTCGAA 1 cut(s) 284
BstC8I GCNNGC 4 cut(s) 153, 225, 574, 663
BstDEI CTNAG 1 cut(s) 170
BstDSI CCRYGG 1 cut(s) 69
BstF5I GGATG 2 cut(s) 445, 529
BstKTI GATC 2 cut(s) 253, 435
BstMBI GATC 2 cut(s) 250, 432
BstMCI CGRYCG 1 cut(s) 82
BstMWI GCNNNNNNNGC 4 cut(s) 56, 65, 152, 592
BstNSI RCATGY 2 cut(s) 518, 665
BstSCI CCNGG 1 cut(s) 451
BstSFI CTRYAG 2 cut(s) 18, 596
BstV1I GCAGC 3 cut(s) 186, 582, 744
BstV2I GAAGAC 1 cut(s) 38
BstXI CCANNNNNNTGG 1 cut(s) 541
BstZI CGGCCG 1 cut(s) 79
BsuRI GGCC 2 cut(s) 81, 545
BtgI CCRYGG 1 cut(s) 69
BtgZI GCGATG 1 cut(s) 764
BtsCI GGATG 2 cut(s) 445, 529
Cac8I GCNNGC 4 cut(s) 153, 225, 574, 663
CaiI CAGNNNCTG 1 cut(s) 193
Cfr13I GGNCC 2 cut(s) 63, 103
CseI GACGC 1 cut(s) 592
CviAII CATG 4 cut(s) 11, 70, 515, 662
DdeI CTNAG 1 cut(s) 170
DpnI GATC 2 cut(s) 252, 434
DpnII GATC 2 cut(s) 250, 432
EaeI YGGCCR 2 cut(s) 79, 543
EagI CGGCCG 1 cut(s) 79
Eam1104I CTCTTC 1 cut(s) 318
EarI CTCTTC 1 cut(s) 318
EciI GGCGGA 1 cut(s) 170
EclXI CGGCCG 1 cut(s) 79
Eco130I CCWWGG 1 cut(s) 69
Eco47I GGWCC 2 cut(s) 63, 103
Eco52I CGGCCG 1 cut(s) 79
Eco57I CTGAAG 3 cut(s) 215, 299, 405
Eco88I CYCGRG 1 cut(s) 203
EcoT14I CCWWGG 1 cut(s) 69
ErhI CCWWGG 1 cut(s) 69
FaeI CATG 4 cut(s) 14, 73, 518, 665
FaqI GGGAC 1 cut(s) 711
FatI CATG 4 cut(s) 10, 69, 514, 661
FauI CCCGC 1 cut(s) 144
Fnu4HI GCNGC 6 cut(s) 82, 167, 200, 596, 747, 758
FokI GGATG 2 cut(s) 452, 536
Fsp4HI GCNGC 6 cut(s) 82, 167, 200, 596, 747, 758
FspBI CTAG 2 cut(s) 300, 768
GluI GCNGC 6 cut(s) 82, 167, 200, 596, 747, 758
GsuI CTGGAG 2 cut(s) 161, 203
HaeIII GGCC 2 cut(s) 81, 545
HapII CCGG 1 cut(s) 452
HgaI GACGC 1 cut(s) 592
Hin1I GRCGYC 1 cut(s) 584
Hin1II CATG 4 cut(s) 14, 73, 518, 665
HinfI GANTC 7 cut(s) 160, 190, 218, 281, 303, 323, 448
HpaII CCGG 1 cut(s) 452
HphI GGTGA 2 cut(s) 49, 80
Hpy166II GTNNAC 2 cut(s) 63, 690
Hpy188I TCNGA 4 cut(s) 130, 195, 217, 318
Hpy188III TCNNGA 3 cut(s) 205, 248, 295
Hpy8I GTNNAC 2 cut(s) 63, 690
Hpy99I CGWCG 3 cut(s) 113, 134, 277
HpyAV CCTTC 1 cut(s) 313
HpyCH4III ACNGT 4 cut(s) 37, 113, 124, 277
HpyCH4V TGCA 3 cut(s) 598, 665, 760
HpyF10VI GCNNNNNNNGC 4 cut(s) 56, 65, 152, 592
HpyF3I CTNAG 1 cut(s) 170
Hsp92I GRCGYC 1 cut(s) 584
Hsp92II CATG 4 cut(s) 14, 73, 518, 665
Kzo9I GATC 2 cut(s) 250, 432
LguI GCTCTTC 1 cut(s) 318
LmnI GCTCC 5 cut(s) 14, 184, 511, 592, 723
Lsp1109I GCAGC 3 cut(s) 186, 582, 744
MaeI CTAG 2 cut(s) 300, 768
MalI GATC 2 cut(s) 252, 434
MboI GATC 2 cut(s) 250, 432
MboII GAAGA 4 cut(s) 43, 146, 305, 422
MlsI TGGCCA 1 cut(s) 545
MluCI AATT 6 cut(s) 399, 535, 603, 635, 740, 777
MluNI TGGCCA 1 cut(s) 545
MlyI GAGTC 1 cut(s) 227
MmeI TCCRAC 1 cut(s) 153
Mox20I TGGCCA 1 cut(s) 545
MroXI GAANNNNTTC 3 cut(s) 124, 290, 674
MscI TGGCCA 1 cut(s) 545
MseI TTAA 1 cut(s) 737
Msp20I TGGCCA 1 cut(s) 545
MspI CCGG 1 cut(s) 452
MspR9I CCNGG 1 cut(s) 453
MwoI GCNNNNNNNGC 4 cut(s) 56, 65, 152, 592
NciI CCSGG 1 cut(s) 453
NcoI CCATGG 1 cut(s) 69
NdeII GATC 2 cut(s) 250, 432
NlaIII CATG 4 cut(s) 14, 73, 518, 665
NlaIV GGNNCC 1 cut(s) 725
NspI RCATGY 2 cut(s) 518, 665
NspV TTCGAA 1 cut(s) 284
PaeI GCATGC 1 cut(s) 665
PaeR7I CTCGAG 1 cut(s) 203
PciSI GCTCTTC 1 cut(s) 318
PcsI WCGNNNNNNNCGW 2 cut(s) 105, 281
PdmI GAANNNNTTC 3 cut(s) 124, 290, 674
PfeI GAWTC 6 cut(s) 160, 190, 281, 303, 323, 448
PflMI CCANNNNNTGG 1 cut(s) 423
PkrI GCNGC 6 cut(s) 83, 168, 201, 597, 748, 759
PleI GAGTC 1 cut(s) 226
PpsI GAGTC 1 cut(s) 226
PspN4I GGNNCC 1 cut(s) 725
PspPI GGNCC 2 cut(s) 63, 103
PstI CTGCAG 1 cut(s) 600
PstNI CAGNNNCTG 1 cut(s) 193
SapI GCTCTTC 1 cut(s) 318
SaqAI TTAA 1 cut(s) 737
SatI GCNGC 6 cut(s) 82, 167, 200, 596, 747, 758
Sau3AI GATC 2 cut(s) 250, 432
Sau96I GGNCC 2 cut(s) 63, 103
SchI GAGTC 1 cut(s) 227
ScrFI CCNGG 1 cut(s) 453
SfcI CTRYAG 2 cut(s) 18, 596
Sfr274I CTCGAG 1 cut(s) 203
SfuI TTCGAA 1 cut(s) 284
SinI GGWCC 2 cut(s) 63, 103
SlaI CTCGAG 1 cut(s) 203
SmlI CTYRAG 1 cut(s) 203
SmoI CTYRAG 1 cut(s) 203
SphI GCATGC 1 cut(s) 665
Sse9I AATT 6 cut(s) 399, 535, 603, 635, 740, 777
SsiI CCGC 8 cut(s) 66, 82, 151, 155, 166, 227, 731, 746
SspMI CTAG 2 cut(s) 300, 768
StyD4I CCNGG 1 cut(s) 451
StyI CCWWGG 1 cut(s) 69
TaaI ACNGT 4 cut(s) 37, 113, 124, 277
TaqI TCGA 3 cut(s) 204, 284, 462
TasI AATT 6 cut(s) 399, 535, 603, 635, 740, 777
TauI GCSGC 3 cut(s) 84, 169, 749
TfiI GAWTC 6 cut(s) 160, 190, 281, 303, 323, 448
Tru1I TTAA 1 cut(s) 737
Tru9I TTAA 1 cut(s) 737
TseI GCWGC 3 cut(s) 199, 595, 757
TspDTI ATGAA 1 cut(s) 423
TspGWI ACGGA 1 cut(s) 452
Van91I CCANNNNNTGG 1 cut(s) 423
VpaK11BI GGWCC 2 cut(s) 63, 103
XapI RAATTY 1 cut(s) 740
XceI RCATGY 2 cut(s) 518, 665
XhoI CTCGAG 1 cut(s) 203
XmnI GAANNNNTTC 3 cut(s) 124, 290, 674
XspI CTAG 2 cut(s) 300, 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.