MD15G1351500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0013484
Physical Location & Seq
Reverse (-)
41806765 .. 41809025
2261 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1351500.v1.1.491

Sequence Viewer

Length: 798 bp
ATGGGAGGACATGGAACGATAGAGGTGGCCAAGACGGTGATGGAGGTTGCCGACGTGGCGTGGACCGCCTTGGAACACGGCCGCCATCACCTCCACCACGACGACCACGAGGGGACCAAGTGTGCGGTTTCCGACGAAGAACTGGAGGCTCTGCGGGCGGAGAATCGGCGGCTGAGGAACTCCCTGGAGCAGAATCTCAAGCTGCTTCAGAACCTGTCCGAGTCGCCTGCGGTGTTGGACGATTGCCCTCCTGATCTGCATGCTCGGTTAGTGGCGACGGTGGAATCGAACAGCTTCTTGACTCGAATCGAAGCTCTTCAGAAGGAATCCATTGATGAATATCAGTTTCCTTTCAAGCCAGCCACAGAAAGCGATTTGGAGAAAGCTGAAGTTCTAATCAAAGTCGACAATGAAGAACCCAGTTGGTGGGTGTGGATCACGGATGACATGATTCCGGGTAAAGTCGAGGAGAGGAGTGGGATTGACAATGAGAACTATGTTGTTGTTAGTGAGGAGCATGTGGTGGATGGGGTTGCCAATTTTATGGCTAAGTGTATTGTGTCGAACCCAAAAGCTCGGAATTTGACGCCGGAGGAGCTGCAGAAAATTGTAGCTAAAGCGATGGGTGGTGTCAGCAAATTGGAGAAGGTATTGGGTATTTGGCATGCGGGGAAGTTGTTCTATGCGTTGTCCACCTGGGGACTGGCTCTAGCTGGATTGTATAGGAGCCGTGCGGTATTAAAATTTGCCGCAATGGGGCTGCACACAAGCAGCAAAGCGATTATGAGAGCAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

266

Amino Acids

29.52

Weight (kDa)

5.4

Isoelectric Point (pI)

37.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44770 AT1G44770
fragaria_vesca FvH4_2g26730
malus_domestica MD08G1165700.v1.1 MD15G1351500.v1.1
pyrus_communis pycom12654g00110 pycom15g31390
rosa_chinensis RchiOBHm_Chr6g0295611
rosa_laevigata RLG00000011785
rosa_multiflora Rmu_sc0013988.1_g000004
rosa_roxburghii Rroxscaffold_7G00172320
rosa_rugosa Rorug06G0252700 Rorug06G0252800
rosa_samantha Rh6AG365800 Rh6BG373900 Rh6CG380200 Rh6DG366800
rosa_wichuraiana Rw6G031920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 426
AccI GTMKAC 1 cut(s) 405
AclWI GGATC 1 cut(s) 443
AcoI YGGCCR 2 cut(s) 27, 79
AcsI RAATTY 2 cut(s) 580, 743
AcuI CTGAAG 3 cut(s) 191, 302, 408
AcyI GRCGYC 1 cut(s) 587
AfiI CCNNNNNNNGG 2 cut(s) 426, 756
AgsI TTSAA 1 cut(s) 355
AjiI CACGTC 1 cut(s) 55
AjnI CCWGG 2 cut(s) 183, 695
AluBI AGCT 8 cut(s) 202, 294, 314, 386, 575, 598, 614, 713
AluI AGCT 8 cut(s) 202, 294, 314, 386, 575, 598, 614, 713
AlwI GGATC 1 cut(s) 443
AlwNI CAGNNNCTG 1 cut(s) 214
AoxI GGCC 2 cut(s) 27, 79
ApeKI GCWGC 4 cut(s) 202, 598, 760, 771
ApoI RAATTY 2 cut(s) 580, 743
Asp700I GAANNNNTTC 3 cut(s) 293, 315, 677
AspS9I GGNCC 2 cut(s) 63, 114
AsuC2I CCSGG 1 cut(s) 456
AsuHPI GGTGA 2 cut(s) 49, 80
AvaII GGWCC 2 cut(s) 63, 114
BalI TGGCCA 1 cut(s) 29
BauI CACGAG 1 cut(s) 107
BbvCI CCTCAGC 1 cut(s) 173
BbvI GCAGC 4 cut(s) 189, 585, 747, 783
BccI CCATC 4 cut(s) 34, 93, 521, 616
BceAI ACGGC 2 cut(s) 94, 714
BcgI CGANNNNNNTGC 2 cut(s) 209, 243
BciT130I CCWGG 2 cut(s) 185, 697
BcnI CCSGG 1 cut(s) 456
BfaI CTAG 1 cut(s) 710
BfmI CTRYAG 1 cut(s) 599
BglI GCCNNNNNGGC 1 cut(s) 56
BisI GCNGC 7 cut(s) 82, 170, 203, 599, 750, 761, 772
BlsI GCNGC 7 cut(s) 83, 171, 204, 600, 751, 762, 773
Bme1390I CCNGG 3 cut(s) 185, 456, 697
Bme18I GGWCC 2 cut(s) 63, 114
BmgBI CACGTC 1 cut(s) 55
BmgT120I GGNCC 2 cut(s) 63, 114
BmiI GGNNCC 2 cut(s) 115, 728
BmrFI CCNGG 3 cut(s) 185, 456, 697
BmrI ACTGGG 1 cut(s) 414
BmuI ACTGGG 1 cut(s) 414
BpmI CTGGAG 2 cut(s) 164, 206
Bpu10I CCTNAGC 1 cut(s) 173
BpuEI CTTGAG 1 cut(s) 182
BpuMI CCSGG 1 cut(s) 456
BsaBI GATNNNNATC 1 cut(s) 339
BsaHI GRCGYC 1 cut(s) 587
BsaJI CCNNGG 3 cut(s) 69, 183, 696
Bsc4I CCNNNNNNNGG 2 cut(s) 426, 756
Bse1I ACTGG 3 cut(s) 147, 420, 708
Bse3DI GCAATG 2 cut(s) 759, 798
Bse8I GATNNNNATC 1 cut(s) 339
BseBI CCWGG 2 cut(s) 185, 697
BseDI CCNNGG 3 cut(s) 69, 183, 696
BseGI GGATG 2 cut(s) 448, 532
BseJI GATNNNNATC 1 cut(s) 339
BseLI CCNNNNNNNGG 2 cut(s) 426, 756
BseMI GCAATG 2 cut(s) 759, 798
BseMII CTCAG 1 cut(s) 164
BseNI ACTGG 3 cut(s) 147, 420, 708
BseRI GAGGAG 4 cut(s) 482, 487, 527, 608
BseX3I CGGCCG 1 cut(s) 79
BseXI GCAGC 4 cut(s) 189, 585, 747, 783
BsgI GTGCAG 1 cut(s) 746
Bsh1285I CGRYCG 1 cut(s) 82
BshFI GGCC 2 cut(s) 29, 81
BsiEI CGRYCG 1 cut(s) 82
BsiSI CCGG 2 cut(s) 455, 590
BslFI GGGAC 2 cut(s) 127, 714
BslI CCNNNNNNNGG 2 cut(s) 426, 756
BsmFI GGGAC 2 cut(s) 127, 714
BsnI GGCC 2 cut(s) 29, 81
Bsp143I GATC 2 cut(s) 253, 435
BspANI GGCC 2 cut(s) 29, 81
BspCNI CTCAG 1 cut(s) 165
BspLI GGNNCC 2 cut(s) 115, 728
BspMAI CTGCAG 1 cut(s) 603
BspPI GGATC 1 cut(s) 443
BspQI GCTCTTC 1 cut(s) 321
BsrDI GCAATG 2 cut(s) 759, 798
BsrI ACTGG 3 cut(s) 147, 420, 708
BssECI CCNNGG 3 cut(s) 69, 183, 696
BssMI GATC 2 cut(s) 253, 435
BssNI GRCGYC 1 cut(s) 587
BssSI CACGAG 1 cut(s) 107
BssT1I CCWWGG 1 cut(s) 69
Bst2BI CACGAG 1 cut(s) 107
Bst2UI CCWGG 2 cut(s) 185, 697
Bst4CI ACNGT 2 cut(s) 37, 280
Bst6I CTCTTC 1 cut(s) 321
BstACI GRCGYC 1 cut(s) 587
BstC8I GCNNGC 5 cut(s) 156, 228, 261, 360, 666
BstDEI CTNAG 2 cut(s) 173, 549
BstF5I GGATG 2 cut(s) 448, 532
BstKTI GATC 2 cut(s) 256, 438
BstMBI GATC 2 cut(s) 253, 435
BstMCI CGRYCG 1 cut(s) 82
BstMWI GCNNNNNNNGC 4 cut(s) 56, 65, 155, 595
BstNI CCWGG 2 cut(s) 185, 697
BstNSI RCATGY 3 cut(s) 263, 521, 668
BstSCI CCNGG 3 cut(s) 183, 454, 695
BstSFI CTRYAG 1 cut(s) 599
BstV1I GCAGC 4 cut(s) 189, 585, 747, 783
BstXI CCANNNNNNTGG 1 cut(s) 544
BstZI CGGCCG 1 cut(s) 79
BsuRI GGCC 2 cut(s) 29, 81
BtgZI GCGATG 1 cut(s) 635
BtrI CACGTC 1 cut(s) 55
BtsCI GGATG 2 cut(s) 448, 532
Cac8I GCNNGC 5 cut(s) 156, 228, 261, 360, 666
CaiI CAGNNNCTG 1 cut(s) 214
Cfr13I GGNCC 2 cut(s) 63, 114
CseI GACGC 1 cut(s) 595
CviAII CATG 5 cut(s) 11, 260, 448, 518, 665
DdeI CTNAG 2 cut(s) 173, 549
DpnI GATC 2 cut(s) 255, 437
DpnII GATC 2 cut(s) 253, 435
EaeI YGGCCR 2 cut(s) 27, 79
EagI CGGCCG 1 cut(s) 79
Eam1104I CTCTTC 1 cut(s) 321
EarI CTCTTC 1 cut(s) 321
EciI GGCGGA 1 cut(s) 173
EclXI CGGCCG 1 cut(s) 79
Eco130I CCWWGG 1 cut(s) 69
Eco47I GGWCC 2 cut(s) 63, 114
Eco52I CGGCCG 1 cut(s) 79
Eco57I CTGAAG 3 cut(s) 191, 302, 408
EcoRII CCWGG 2 cut(s) 183, 695
EcoT14I CCWWGG 1 cut(s) 69
ErhI CCWWGG 1 cut(s) 69
FaeI CATG 5 cut(s) 14, 263, 451, 521, 668
FaqI GGGAC 2 cut(s) 127, 714
FatI CATG 5 cut(s) 10, 259, 447, 517, 664
FauI CCCGC 2 cut(s) 147, 661
FblI GTMKAC 1 cut(s) 405
Fnu4HI GCNGC 7 cut(s) 82, 170, 203, 599, 750, 761, 772
FokI GGATG 2 cut(s) 455, 539
Fsp4HI GCNGC 7 cut(s) 82, 170, 203, 599, 750, 761, 772
FspBI CTAG 1 cut(s) 710
GluI GCNGC 7 cut(s) 82, 170, 203, 599, 750, 761, 772
GsuI CTGGAG 2 cut(s) 164, 206
HaeIII GGCC 2 cut(s) 29, 81
HapII CCGG 2 cut(s) 455, 590
HgaI GACGC 1 cut(s) 595
Hin1I GRCGYC 1 cut(s) 587
Hin1II CATG 5 cut(s) 14, 263, 451, 521, 668
HincII GTYRAC 1 cut(s) 406
HindII GTYRAC 1 cut(s) 406
HinfI GANTC 8 cut(s) 163, 193, 221, 284, 301, 306, 326, 451
HpaII CCGG 2 cut(s) 455, 590
HphI GGTGA 2 cut(s) 49, 80
Hpy166II GTNNAC 3 cut(s) 63, 406, 693
Hpy188I TCNGA 5 cut(s) 133, 210, 220, 321, 579
Hpy188III TCNNGA 2 cut(s) 251, 298
Hpy8I GTNNAC 3 cut(s) 63, 406, 693
Hpy99I CGWCG 4 cut(s) 56, 104, 137, 280
HpyAV CCTTC 2 cut(s) 316, 640
HpyCH4III ACNGT 2 cut(s) 37, 280
HpyCH4IV ACGT 1 cut(s) 54
HpyCH4V TGCA 3 cut(s) 259, 601, 763
HpyF10VI GCNNNNNNNGC 4 cut(s) 56, 65, 155, 595
HpyF3I CTNAG 2 cut(s) 173, 549
HpySE526I ACGT 1 cut(s) 54
Hsp92I GRCGYC 1 cut(s) 587
Hsp92II CATG 5 cut(s) 14, 263, 451, 521, 668
Kzo9I GATC 2 cut(s) 253, 435
LguI GCTCTTC 1 cut(s) 321
LmnI GCTCC 4 cut(s) 187, 514, 595, 726
Lsp1109I GCAGC 4 cut(s) 189, 585, 747, 783
MaeI CTAG 1 cut(s) 710
MaeII ACGT 1 cut(s) 54
MalI GATC 2 cut(s) 255, 437
MboI GATC 2 cut(s) 253, 435
MboII GAAGA 3 cut(s) 149, 308, 425
MlsI TGGCCA 1 cut(s) 29
MluCI AATT 5 cut(s) 538, 580, 606, 638, 743
MluNI TGGCCA 1 cut(s) 29
MlyI GAGTC 2 cut(s) 230, 295
MmeI TCCRAC 2 cut(s) 156, 216
Mox20I TGGCCA 1 cut(s) 29
MroXI GAANNNNTTC 3 cut(s) 293, 315, 677
MscI TGGCCA 1 cut(s) 29
MseI TTAA 1 cut(s) 740
Msp20I TGGCCA 1 cut(s) 29
MspI CCGG 2 cut(s) 455, 590
MspR9I CCNGG 3 cut(s) 185, 456, 697
MvaI CCWGG 2 cut(s) 185, 697
MwoI GCNNNNNNNGC 4 cut(s) 56, 65, 155, 595
NciI CCSGG 1 cut(s) 456
NdeII GATC 2 cut(s) 253, 435
NlaIII CATG 5 cut(s) 14, 263, 451, 521, 668
NlaIV GGNNCC 2 cut(s) 115, 728
NspI RCATGY 3 cut(s) 263, 521, 668
PaeI GCATGC 2 cut(s) 263, 668
PciSI GCTCTTC 1 cut(s) 321
PcsI WCGNNNNNNNCGW 2 cut(s) 105, 284
PdmI GAANNNNTTC 3 cut(s) 293, 315, 677
PfeI GAWTC 6 cut(s) 163, 193, 284, 306, 326, 451
PflMI CCANNNNNTGG 1 cut(s) 426
PkrI GCNGC 7 cut(s) 83, 171, 204, 600, 751, 762, 773
PleI GAGTC 2 cut(s) 229, 295
PpsI GAGTC 2 cut(s) 229, 295
Psp6I CCWGG 2 cut(s) 183, 695
PspGI CCWGG 2 cut(s) 183, 695
PspN4I GGNNCC 2 cut(s) 115, 728
PspPI GGNCC 2 cut(s) 63, 114
PstI CTGCAG 1 cut(s) 603
PstNI CAGNNNCTG 1 cut(s) 214
SalI GTCGAC 1 cut(s) 404
SapI GCTCTTC 1 cut(s) 321
SaqAI TTAA 1 cut(s) 740
SatI GCNGC 7 cut(s) 82, 170, 203, 599, 750, 761, 772
Sau3AI GATC 2 cut(s) 253, 435
Sau96I GGNCC 2 cut(s) 63, 114
SchI GAGTC 2 cut(s) 230, 295
ScrFI CCNGG 3 cut(s) 185, 456, 697
SfcI CTRYAG 1 cut(s) 599
SinI GGWCC 2 cut(s) 63, 114
SmlI CTYRAG 1 cut(s) 197
SmoI CTYRAG 1 cut(s) 197
SphI GCATGC 2 cut(s) 263, 668
Sse9I AATT 5 cut(s) 538, 580, 606, 638, 743
SspMI CTAG 1 cut(s) 710
StyD4I CCNGG 3 cut(s) 183, 454, 695
StyI CCWWGG 1 cut(s) 69
TaaI ACNGT 2 cut(s) 37, 280
TaiI ACGT 1 cut(s) 57
TaqI TCGA 6 cut(s) 287, 304, 309, 405, 465, 563
TasI AATT 5 cut(s) 538, 580, 606, 638, 743
TauI GCSGC 3 cut(s) 84, 172, 752
TfiI GAWTC 6 cut(s) 163, 193, 284, 306, 326, 451
Tru1I TTAA 1 cut(s) 740
Tru9I TTAA 1 cut(s) 740
TseI GCWGC 4 cut(s) 202, 598, 760, 771
TspDTI ATGAA 2 cut(s) 351, 426
TspGWI ACGGA 1 cut(s) 455
Van91I CCANNNNNTGG 1 cut(s) 426
VpaK11BI GGWCC 2 cut(s) 63, 114
XapI RAATTY 2 cut(s) 580, 743
XceI RCATGY 3 cut(s) 263, 521, 668
XcmI CCANNNNNNNNNTGG 2 cut(s) 37, 700
XmiI GTMKAC 1 cut(s) 405
XmnI GAANNNNTTC 3 cut(s) 293, 315, 677
XspI CTAG 1 cut(s) 710
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.