pycom12654g00110

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012654
OG0013484
Physical Location & Seq
Reverse (-)
126838 .. 128667
1830 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12654g00110.1

Sequence Viewer

Length: 804 bp
ATGGGAGGACGTGGAGCTATAGAGGTGGCGAAGACGGTGGTGGAGGTTGCTGACGTGGCGTGGACCGCCATGGAACACGGCCGCCATCACCTCCACCACGAGGACCACGACGGCGAGGGAACCAAGTGCGCGGTTTCCGACGAAGAACTGGAGGCTCTGCGGGCGGAGAATCGGCGGCTGAGGAACTCGCTGGAGCAGAATCTGAAGCTGCTCGAGAACCTCTCCGAGTCGCCTGCGGTTTTGGACGATTGCCCTCCTGATCTGTATGCTCGGTTAGTGGCGACGGTGGATTCGAACAGCTTCTTGACTAGAATCAAAGCTCTTCAGAAGGAGTCCATTGATGATTATCAGTTTCCTTTCAAACCAGCCACAGGAAGTGATTTGGAGAAAGCTGAAGTTCTAATCAAAATTGACAATGAAGAACCCAGTCGGTGGGTGTGGATCACGGATGATATGATTCCGGGTAAAGTCGAGGAGAGGAGTGGGATTGACAATGAGAGCTATGTTGTTGTTAGTGAGGAGCATGTGGTGGATGGGATTGCCAATTTTATGGCCAAGTGTATTGTGTCAAACCCGAAAGCTCACAACTTGACGCCAGAGGAGCTGCAGAAAATTGTAGCTAAAGCGATGGGTGGTGTCAGCAAATTGGAGAAAGTATTGGGCATTTGGCATGCGGGGAAGTTGTTCTATACTTTGTCCACCTGGGGACTTGCTCTAACCGGATTGTATAGGAGCCGTGCGGTATTAAAATTTGCTGCAATGGGGCTGCACACAACTAGCAAAGCAATTATGAGAGCAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

268

Amino Acids

29.63

Weight (kDa)

5.51

Isoelectric Point (pI)

35.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44770 AT1G44770
fragaria_vesca FvH4_2g26730
malus_domestica MD08G1165700.v1.1 MD15G1351500.v1.1
pyrus_communis pycom12654g00110 pycom15g31390
rosa_chinensis RchiOBHm_Chr6g0295611
rosa_laevigata RLG00000011785
rosa_multiflora Rmu_sc0013988.1_g000004
rosa_roxburghii Rroxscaffold_7G00172320
rosa_rugosa Rorug06G0252700 Rorug06G0252800
rosa_samantha Rh6AG365800 Rh6BG373900 Rh6CG380200 Rh6DG366800
rosa_wichuraiana Rw6G031920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 432
AccII CGCG 1 cut(s) 131
AciI CCGC 9 cut(s) 66, 82, 131, 160, 164, 175, 236, 674, 740
AclWI GGATC 1 cut(s) 449
AcoI YGGCCR 2 cut(s) 79, 552
AcsI RAATTY 1 cut(s) 749
AcuI CTGAAG 3 cut(s) 224, 308, 414
AcyI GRCGYC 1 cut(s) 593
AfiI CCNNNNNNNGG 3 cut(s) 100, 371, 432
AgsI TTSAA 1 cut(s) 361
AjiI CACGTC 2 cut(s) 11, 55
AjnI CCWGG 1 cut(s) 701
AluBI AGCT 9 cut(s) 17, 208, 300, 320, 392, 501, 581, 604, 620
AluI AGCT 9 cut(s) 17, 208, 300, 320, 392, 501, 581, 604, 620
AlwI GGATC 1 cut(s) 449
AlwNI CAGNNNCTG 1 cut(s) 202
Ama87I CYCGRG 1 cut(s) 212
AoxI GGCC 2 cut(s) 79, 552
ApeKI GCWGC 4 cut(s) 208, 604, 755, 766
ApoI RAATTY 1 cut(s) 749
ArsI GACNNNNNNTTYG 2 cut(s) 274, 306
Asp700I GAANNNNTTC 2 cut(s) 299, 683
AspLEI GCGC 1 cut(s) 131
AspS9I GGNCC 2 cut(s) 63, 103
AsuC2I CCSGG 1 cut(s) 462
AsuHPI GGTGA 1 cut(s) 80
AsuII TTCGAA 1 cut(s) 293
AvaI CYCGRG 1 cut(s) 212
AvaII GGWCC 2 cut(s) 63, 103
BalI TGGCCA 1 cut(s) 554
BauI CACGAG 1 cut(s) 98
BbsI GAAGAC 1 cut(s) 38
BbvCI CCTCAGC 1 cut(s) 179
BbvI GCAGC 4 cut(s) 195, 591, 742, 753
BccI CCATC 3 cut(s) 93, 527, 622
BceAI ACGGC 3 cut(s) 94, 127, 720
BcgI CGANNNNNNTGC 2 cut(s) 215, 249
BciT130I CCWGG 1 cut(s) 703
BcnI CCSGG 1 cut(s) 462
BfaI CTAG 2 cut(s) 309, 777
BfmI CTRYAG 2 cut(s) 18, 605
BisI GCNGC 6 cut(s) 82, 176, 209, 605, 756, 767
BlsI GCNGC 6 cut(s) 83, 177, 210, 606, 757, 768
Bme1390I CCNGG 2 cut(s) 462, 703
Bme18I GGWCC 2 cut(s) 63, 103
BmeT110I CYCGRG 1 cut(s) 212
BmgBI CACGTC 2 cut(s) 11, 55
BmgT120I GGNCC 2 cut(s) 63, 103
BmiI GGNNCC 2 cut(s) 121, 734
BmrFI CCNGG 2 cut(s) 462, 703
BmrI ACTGGG 1 cut(s) 420
BmuI ACTGGG 1 cut(s) 420
BpiI GAAGAC 1 cut(s) 38
BplI GAGNNNNNCTC 2 cut(s) 206, 238
BpmI CTGGAG 2 cut(s) 170, 212
Bpu10I CCTNAGC 1 cut(s) 179
Bpu14I TTCGAA 1 cut(s) 293
BpuMI CCSGG 1 cut(s) 462
BsaBI GATNNNNATC 1 cut(s) 345
BsaHI GRCGYC 1 cut(s) 593
BsaJI CCNNGG 2 cut(s) 69, 702
BsaWI WCCGGW 1 cut(s) 719
Bsc4I CCNNNNNNNGG 3 cut(s) 100, 371, 432
Bse1I ACTGG 2 cut(s) 153, 426
Bse3DI GCAATG 2 cut(s) 765, 804
Bse8I GATNNNNATC 1 cut(s) 345
BseBI CCWGG 1 cut(s) 703
BseDI CCNNGG 2 cut(s) 69, 702
BseGI GGATG 2 cut(s) 454, 538
BseJI GATNNNNATC 1 cut(s) 345
BseLI CCNNNNNNNGG 3 cut(s) 100, 371, 432
BseMI GCAATG 2 cut(s) 765, 804
BseMII CTCAG 1 cut(s) 170
BseNI ACTGG 2 cut(s) 153, 426
BseRI GAGGAG 4 cut(s) 488, 493, 533, 614
BseX3I CGGCCG 1 cut(s) 79
BseXI GCAGC 4 cut(s) 195, 591, 742, 753
BsgI GTGCAG 1 cut(s) 752
Bsh1236I CGCG 1 cut(s) 131
Bsh1285I CGRYCG 1 cut(s) 82
BshFI GGCC 2 cut(s) 81, 554
BsiEI CGRYCG 1 cut(s) 82
BsiHKCI CYCGRG 1 cut(s) 212
BsiSI CCGG 2 cut(s) 461, 720
BslFI GGGAC 1 cut(s) 720
BslI CCNNNNNNNGG 3 cut(s) 100, 371, 432
BsmFI GGGAC 1 cut(s) 720
BsnI GGCC 2 cut(s) 81, 554
BsoBI CYCGRG 1 cut(s) 212
Bsp119I TTCGAA 1 cut(s) 293
Bsp143I GATC 2 cut(s) 259, 441
Bsp19I CCATGG 1 cut(s) 69
BspACI CCGC 9 cut(s) 66, 82, 131, 160, 164, 175, 236, 674, 740
BspANI GGCC 2 cut(s) 81, 554
BspCNI CTCAG 1 cut(s) 171
BspFNI CGCG 1 cut(s) 131
BspLI GGNNCC 2 cut(s) 121, 734
BspMAI CTGCAG 1 cut(s) 609
BspPI GGATC 1 cut(s) 449
BspQI GCTCTTC 1 cut(s) 327
BspT104I TTCGAA 1 cut(s) 293
BsrDI GCAATG 2 cut(s) 765, 804
BsrI ACTGG 2 cut(s) 153, 426
BssECI CCNNGG 2 cut(s) 69, 702
BssMI GATC 2 cut(s) 259, 441
BssNI GRCGYC 1 cut(s) 593
BssSI CACGAG 1 cut(s) 98
BssT1I CCWWGG 1 cut(s) 69
Bst2BI CACGAG 1 cut(s) 98
Bst2UI CCWGG 1 cut(s) 703
Bst4CI ACNGT 2 cut(s) 37, 286
Bst6I CTCTTC 1 cut(s) 327
BstACI GRCGYC 1 cut(s) 593
BstBI TTCGAA 1 cut(s) 293
BstC8I GCNNGC 3 cut(s) 162, 234, 672
BstDEI CTNAG 1 cut(s) 179
BstDSI CCRYGG 1 cut(s) 69
BstF5I GGATG 2 cut(s) 454, 538
BstFNI CGCG 1 cut(s) 131
BstHHI GCGC 1 cut(s) 131
BstKTI GATC 2 cut(s) 262, 444
BstMBI GATC 2 cut(s) 259, 441
BstMCI CGRYCG 1 cut(s) 82
BstMWI GCNNNNNNNGC 4 cut(s) 56, 65, 161, 601
BstNI CCWGG 1 cut(s) 703
BstNSI RCATGY 2 cut(s) 527, 674
BstSCI CCNGG 2 cut(s) 460, 701
BstSFI CTRYAG 2 cut(s) 18, 605
BstUI CGCG 1 cut(s) 131
BstV1I GCAGC 4 cut(s) 195, 591, 742, 753
BstV2I GAAGAC 1 cut(s) 38
BstXI CCANNNNNNTGG 1 cut(s) 550
BstZI CGGCCG 1 cut(s) 79
BsuRI GGCC 2 cut(s) 81, 554
BtgI CCRYGG 1 cut(s) 69
BtgZI GCGATG 1 cut(s) 641
BtrI CACGTC 2 cut(s) 11, 55
BtsCI GGATG 2 cut(s) 454, 538
Cac8I GCNNGC 3 cut(s) 162, 234, 672
CaiI CAGNNNCTG 1 cut(s) 202
CfoI GCGC 1 cut(s) 131
Cfr13I GGNCC 2 cut(s) 63, 103
CseI GACGC 1 cut(s) 601
CviAII CATG 3 cut(s) 70, 524, 671
DdeI CTNAG 1 cut(s) 179
DpnI GATC 2 cut(s) 261, 443
DpnII GATC 2 cut(s) 259, 441
EaeI YGGCCR 2 cut(s) 79, 552
EagI CGGCCG 1 cut(s) 79
Eam1104I CTCTTC 1 cut(s) 327
EarI CTCTTC 1 cut(s) 327
EciI GGCGGA 1 cut(s) 179
EclXI CGGCCG 1 cut(s) 79
Eco130I CCWWGG 1 cut(s) 69
Eco47I GGWCC 2 cut(s) 63, 103
Eco52I CGGCCG 1 cut(s) 79
Eco57I CTGAAG 3 cut(s) 224, 308, 414
Eco88I CYCGRG 1 cut(s) 212
EcoRII CCWGG 1 cut(s) 701
EcoT14I CCWWGG 1 cut(s) 69
ErhI CCWWGG 1 cut(s) 69
FaeI CATG 3 cut(s) 73, 527, 674
FaqI GGGAC 1 cut(s) 720
FatI CATG 3 cut(s) 69, 523, 670
FauI CCCGC 2 cut(s) 153, 667
Fnu4HI GCNGC 6 cut(s) 82, 176, 209, 605, 756, 767
FokI GGATG 2 cut(s) 461, 545
Fsp4HI GCNGC 6 cut(s) 82, 176, 209, 605, 756, 767
FspBI CTAG 2 cut(s) 309, 777
GlaI GCGC 1 cut(s) 130
GluI GCNGC 6 cut(s) 82, 176, 209, 605, 756, 767
GsuI CTGGAG 2 cut(s) 170, 212
HaeIII GGCC 2 cut(s) 81, 554
HapII CCGG 2 cut(s) 461, 720
HgaI GACGC 1 cut(s) 601
HhaI GCGC 1 cut(s) 131
Hin1I GRCGYC 1 cut(s) 593
Hin1II CATG 3 cut(s) 73, 527, 674
Hin6I GCGC 1 cut(s) 129
HinP1I GCGC 1 cut(s) 129
HinfI GANTC 7 cut(s) 169, 199, 227, 290, 312, 332, 457
HpaII CCGG 2 cut(s) 461, 720
HphI GGTGA 1 cut(s) 80
Hpy166II GTNNAC 2 cut(s) 63, 699
Hpy188I TCNGA 4 cut(s) 139, 204, 226, 327
Hpy188III TCNNGA 3 cut(s) 214, 257, 304
Hpy8I GTNNAC 2 cut(s) 63, 699
Hpy99I CGWCG 3 cut(s) 113, 143, 286
HpyAV CCTTC 1 cut(s) 322
HpyCH4III ACNGT 2 cut(s) 37, 286
HpyCH4IV ACGT 2 cut(s) 10, 54
HpyCH4V TGCA 3 cut(s) 607, 758, 769
HpyF10VI GCNNNNNNNGC 4 cut(s) 56, 65, 161, 601
HpyF3I CTNAG 1 cut(s) 179
HpySE526I ACGT 2 cut(s) 10, 54
Hsp92I GRCGYC 1 cut(s) 593
Hsp92II CATG 3 cut(s) 73, 527, 674
HspAI GCGC 1 cut(s) 129
Kzo9I GATC 2 cut(s) 259, 441
LguI GCTCTTC 1 cut(s) 327
LmnI GCTCC 5 cut(s) 14, 193, 520, 601, 732
Lsp1109I GCAGC 4 cut(s) 195, 591, 742, 753
MaeI CTAG 2 cut(s) 309, 777
MaeII ACGT 2 cut(s) 10, 54
MalI GATC 2 cut(s) 261, 443
MboI GATC 2 cut(s) 259, 441
MboII GAAGA 4 cut(s) 43, 155, 314, 431
MlsI TGGCCA 1 cut(s) 554
MluCI AATT 6 cut(s) 408, 544, 612, 644, 749, 786
MluNI TGGCCA 1 cut(s) 554
MlyI GAGTC 2 cut(s) 236, 341
MmeI TCCRAC 1 cut(s) 162
Mox20I TGGCCA 1 cut(s) 554
MroXI GAANNNNTTC 2 cut(s) 299, 683
MscI TGGCCA 1 cut(s) 554
MseI TTAA 1 cut(s) 746
Msp20I TGGCCA 1 cut(s) 554
MspI CCGG 2 cut(s) 461, 720
MspR9I CCNGG 2 cut(s) 462, 703
MvaI CCWGG 1 cut(s) 703
MvnI CGCG 1 cut(s) 131
MwoI GCNNNNNNNGC 4 cut(s) 56, 65, 161, 601
NciI CCSGG 1 cut(s) 462
NcoI CCATGG 1 cut(s) 69
NdeII GATC 2 cut(s) 259, 441
NlaIII CATG 3 cut(s) 73, 527, 674
NlaIV GGNNCC 2 cut(s) 121, 734
NspI RCATGY 2 cut(s) 527, 674
NspV TTCGAA 1 cut(s) 293
PaeI GCATGC 1 cut(s) 674
PaeR7I CTCGAG 1 cut(s) 212
PciSI GCTCTTC 1 cut(s) 327
PcsI WCGNNNNNNNCGW 2 cut(s) 105, 290
PdmI GAANNNNTTC 2 cut(s) 299, 683
PfeI GAWTC 5 cut(s) 169, 199, 290, 312, 457
PflMI CCANNNNNTGG 1 cut(s) 432
PkrI GCNGC 6 cut(s) 83, 177, 210, 606, 757, 768
PleI GAGTC 2 cut(s) 235, 340
PpsI GAGTC 2 cut(s) 235, 340
Psp6I CCWGG 1 cut(s) 701
PspGI CCWGG 1 cut(s) 701
PspN4I GGNNCC 2 cut(s) 121, 734
PspPI GGNCC 2 cut(s) 63, 103
PstI CTGCAG 1 cut(s) 609
PstNI CAGNNNCTG 1 cut(s) 202
SapI GCTCTTC 1 cut(s) 327
SaqAI TTAA 1 cut(s) 746
SatI GCNGC 6 cut(s) 82, 176, 209, 605, 756, 767
Sau3AI GATC 2 cut(s) 259, 441
Sau96I GGNCC 2 cut(s) 63, 103
SchI GAGTC 2 cut(s) 236, 341
ScrFI CCNGG 2 cut(s) 462, 703
SfcI CTRYAG 2 cut(s) 18, 605
Sfr274I CTCGAG 1 cut(s) 212
SfuI TTCGAA 1 cut(s) 293
SinI GGWCC 2 cut(s) 63, 103
SlaI CTCGAG 1 cut(s) 212
SmlI CTYRAG 1 cut(s) 212
SmoI CTYRAG 1 cut(s) 212
SphI GCATGC 1 cut(s) 674
Sse9I AATT 6 cut(s) 408, 544, 612, 644, 749, 786
SsiI CCGC 9 cut(s) 66, 82, 131, 160, 164, 175, 236, 674, 740
SspMI CTAG 2 cut(s) 309, 777
StyD4I CCNGG 2 cut(s) 460, 701
StyI CCWWGG 1 cut(s) 69
TaaI ACNGT 2 cut(s) 37, 286
TaiI ACGT 2 cut(s) 13, 57
TaqI TCGA 3 cut(s) 213, 293, 471
TasI AATT 6 cut(s) 408, 544, 612, 644, 749, 786
TauI GCSGC 2 cut(s) 84, 178
TfiI GAWTC 5 cut(s) 169, 199, 290, 312, 457
Tru1I TTAA 1 cut(s) 746
Tru9I TTAA 1 cut(s) 746
TseI GCWGC 4 cut(s) 208, 604, 755, 766
TspDTI ATGAA 1 cut(s) 432
TspGWI ACGGA 1 cut(s) 461
Van91I CCANNNNNTGG 1 cut(s) 432
VpaK11BI GGWCC 2 cut(s) 63, 103
XapI RAATTY 1 cut(s) 749
XceI RCATGY 2 cut(s) 527, 674
XhoI CTCGAG 1 cut(s) 212
XmnI GAANNNNTTC 2 cut(s) 299, 683
XspI CTAG 2 cut(s) 309, 777
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.