Rorug06G0252700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0013484
Physical Location & Seq
Forward (+)
39906479 .. 39910600
4122 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0252700.1

Sequence Viewer

Length: 4122 bp
ATGCTGCCATTCAAGGTACTCACCTGGAACTGTCGAGGACTGGTTAACACAGAAACGCAGAGTGCCTTGGTCTCTCTGGTACGTCATGTCAACCCTAGTCTGGTTTTTGTCTCAGAAACTCTTGCCGGACCAAGGCTGATGGAGGACCTGCGAGTTGAAATGGGCTTCTCTGGTTGCATTTGCTTCCCCAAAAAGGATGGTTCAAGAGGTTTGGCTTTATTCTGGTCTTCGGAATTGAAGGTGAGCCTTCGTACCTATTCTGCACATCATATTGATGTTGAGATAGGGTTTCCTGGTGTTGCTGGAGGTTGGCGATTCACAGGCATTTATGGTTTTTCAAGGCATGGTGATAGGGTTCAAACTTGGGACTTGCTTCGTACTCTGGCAGCACAAGTGAATCTGCCATGGTTAATAGCCGGCGATTTCAATGAGATTTTGTGTGCAGCTGACAAATCCGGTGGTCCACCACGGAACGCTGCGATGATGACGCGGTTCAGGGAAGCCTTGGCCGATTGTAATCTGGAGGACATGGGGTTTGTGGGTTCTCGTTTTACTTGGTTTAATCGGTTCACTAAGGAAAGGTTGGATCGTGGTTGTTGCACGCAAGATTGGTGTGCCATTTACCCATACTCTAGGGTTATCACATTACCTCCTAATCGGTCAGATCACAATCCCATTGTGGTGGAGATAGCTGCTGCACCGGTTGTCAGAGTGCCAAGACTCAAGCGCTTTCGGTTTGAGAACATGTGGATGCAGCATGGCGATTGCGCTGCTGTTATTCAGAAGGGATGGTCCATTCCCTCTATTGGTGAACCTATGAAGCAAGTTGGGTTGAAAATTGGTTCAACGGGCAGGTTGTTAATGGAATTGGATGCAGGAACATTTCGACAGCGGAAAACAGAAATGCATCTCTTGCAATCCAAGATGGATATGTTGATGAAACTCCCTTATGATCCTATTCATTTTGAGCAGCAAAAAACTCTTCAATTTAGATATAATGAACTTCTATCTTTGGATGAAGCTTATTGGCGTCAGAGATCAAGGGTTTTATGGTTGAAAGAAGGTGATCGTAATTCAGCTTTTTTTCACAGGAAAGCGTCTAACAGACGCAGTCGTAATAAGGTGGTAGGTATCACTGATTTGGGAGGACAATGGCACACTGACCCAGCACAAGTGGCTTCAGTGTTTGTTGATTACTATGAAAATATTTTTCACTCTGAGGGTTCTGATCCTGCAGCTCTTGCAGCAGTTCTTGCACATATTCATCCTTGTATTGATAATGATATGAATTCTTCTCTATTAGCTCCATATTCAGACGAGGAAATCAAGAAGGCTTTATTCCAGATGCATCCGTCAAAATCCCCTGGACCTGACGGTATGTCTCCTGCTTTTTTCCAAAAGCATTGGGCAATTGTTCAATTTGATGTGTGTTTAGCTGTGAGGACTTTTTTAAACACAGGTCTTCTGGATTCTGAGAGCAATTTTACTCATTTGTCTCTTATTCCTAAAATTAAAGAGCCTAAAACTGCAGCTGATTTCAGGCCTATAGCCCTTTGCAATGTGGTGTATAAAATTGCTTCTAAGGTGCTTGCTAACAGATTGAAAGTATTATTGCCTTCTATTATCTCTCCTCTTCAAAGTGCTTTTGTTCCTGGCCGTTTGATATCGGATAACACTCTTGTTGCATCTGAAGTTGCTCATTTTATGCATAAGCTCCGACATCAGAATGAGGGTTTCTTCTCCTTTAAGTTAGATATAAGTAAAGCATACGACAGATTGGAGTGGACTTTCTTGATCTCTATTTTATCTAAAATGGGGTTTGCACAAAAGTGGATTGACACTGTTCTTTGTTCCATTAGAACAGTGCGTTATTCTATTCTGTTACATGGTAAACCTACAGGTTATATTACTCCCTCCAGGGGCATCAGGCAGGGGGACCCTCTTTCTCCATACTTATTCATTCTGTGTGTTGAAGGTCTCTCGTCCCTTATTTCTCATGCAGTATCCCAGGGTACACTGAAGGGGCTGAAGATGAGTCCTCAAGCCCCAACTATTCATCATTTGCTATTTGCCGATGATAGCTTTCTATTCGGAGAAGCCTCTGTGGTGGAATGCTCTACATTCAAGTCTATTTTGAATGTGTATGAGCATGCTTCTGGCCAAAGGATTAATTTAGAGAAGAGCAGTGTTGTCTTTAGCAGGAATGTGCATCCAGATACTAAAGATAGTTTAGCTTCTATTTTGGGGGTACAGTGTGTGGAGGACCATGGCCGATATCTAGGGCTTCCACTTCATGTGGGCAAATCAAAAGTGGCAATTTTTTCTTATCTTAAGGAAAGATTAACTAAGAAGCTGATCAGCTGGCGCACAAAAATCCTGAGTTCTGCGGGGAAGGAGATTCTGATTAAGGCAGTGGCTCAAGTTATTCCAACGTATGTTATGAGTTGTTATATGCTTCCTAAAGGTCTTTGTGATGATCTCCACCACCTTTGTGCTCAATTTTTTTGGGGAGGATCAGATTCTAATCGCAAAATTCATTGGCGGTCATGGGATAGGATGTGCCTGTCTAAAAGTGAAGGAGGTTTAGGTTTCAAGAATCTGCATGCTCATAATTTATCTCTGCTTGCAAAACAGGGATGGAGATTAATCACTAATCCTAATTCCTTGTTGTCTCGTTTGCTCAAGGCTAGGTATTTTCCGAATGGTTCTTTTTTGGATGCAGCTATGGGGGATTCCCCCTCGTATGCATGGAGAAGCATTATGGAAGCTAGAACTGTCCTTCAGGCTAGATTACTTTGGCAGGTTGGTAATGGCTCCTCTATTCATGTTTGGAGTGATCAGTGGATTCCAACTGTTTCTCCTCATCAACTCTGTAAACCGGCTGCTTGTAATATTGAGCTAGTCTCTTCCTTGATTGATGCGGATACCAAAAGTTGGAGGGTTGATGTTTTGCAACGTCTATTCTCTCAAGAAGTTGTTGATGCTATCTTGTGTGTTCCTCTCAGTCATAGAAATAGAAGCGATCGTTTGAGCTGGAAGCTTCGGAATAAGGGTAAGTTTTCTTCTAAAATTGCCTACTATGTAGCTCGGGAGGTGGTGATGGGGGATGTCTTTGCTTCCTCCTCTATTGGTGATCCTTTTCGTATTCTCTGGAAGTCACTTTGGAAAGCTAAGATCCCTGGGAAGGTCTCTATATGTATCTGGCGAGCTTGTCACAATGTGTTACCAACTAGAGAGAGTTTGAGTAAGAAGGGTTACACTGGTGATATGGGATGCCTGCTTTGCCGTCACCAGGTTGAGAGTGTTGGCCATGTATTATGTGGGTGCCCTACTGCGCAGGAAATCCTTATTGCTCCTCCTTTTTCAATTCAGGTTCCTATTACTCATTCTTTTATTTTTAAGGAATGGTTACTTGAGCAAGCATCTACTCTTTCTTATGAAAATTTTTCTAAGCTGTTGATGCTTTTATGGGGTCTTTGGAAGAATAGGAATGATAAATTGTGGAATGATAATGCTAAAAACGCTTCTACTATTGTTGCTTGTACTATGGCTTGGTATGAAGAGTTCCTTCAAGCTAATAGGGATACGATTATGGTAATCGATAAGAGGACGAAAGCTAGAGCACATTGGTCCCCTCCCATGAGTGGCTTATTGTGCATGAATGTTGATGGTGCTTATGTCTCTACTCTACAACATGGAGGCATTGGCGGTGTTCTGCGTAATGAGAAGGGTGAGTTCATTGCAGGCTTTGCTTATAGAGTGACTAATGTGTCCTCTGCCTACCATGCTGAGTTGCTTGCTATTAAATCTGGGCTGGAGCTTATTCAAGCGCTGGGTGTGTCTAATGTTGCTATAATGAGTGATTGCTCAGAGGCAGTGAAGGCTGTGACTGCAGAGGATCATGACTTCTCTACTTTGGGAATTATTGTTGAAGAAATTCAAGAATTGCTAGGTGATTTGCTTTCTATAGGTGTTCATCATACTTATAGGTCCGCTAATCATGTTGCTCATAGACTAGCAGGATTTGGTTTTGATTCTGACATTCATATGGAATGGTTTTCTCAAGCTCCAGAGTGTGTCCTGGATGCCCTACAGTACGATTGTAATCGTATAATTCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1373

Amino Acids

154.36

Weight (kDa)

8.96

Isoelectric Point (pI)

39.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 7 - 223 2e-12 Endonuclease/Exonuclease/phosphatase family
RVT_1 PF00078 507 - 762 2e-28 Reverse transcriptase (RNA-dependent DNA polymerase)
zf-RVT PF13966 1050 - 1115 1.3e-14 zinc-binding in reverse transcriptase
RVT_3 PF13456 1221 - 1340 9.2e-24 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44770 AT1G44770
fragaria_vesca FvH4_2g26730
malus_domestica MD08G1165700.v1.1 MD15G1351500.v1.1
pyrus_communis pycom12654g00110 pycom15g31390
rosa_chinensis RchiOBHm_Chr6g0295611
rosa_laevigata RLG00000011785
rosa_multiflora Rmu_sc0013988.1_g000004
rosa_roxburghii Rroxscaffold_7G00172320
rosa_rugosa Rorug06G0252700 Rorug06G0252800
rosa_samantha Rh6AG365800 Rh6BG373900 Rh6CG380200 Rh6DG366800
rosa_wichuraiana Rw6G031920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 3769
Acc16I TGCGCA 1 cut(s) 3336
Acc36I ACCTGC 3 cut(s) 156, 843, 2791
AccB1I GGYRCC 1 cut(s) 3324
AccB7I CCANNNNNTGG 1 cut(s) 2934
AccII CGCG 1 cut(s) 490
AciI CCGC 7 cut(s) 490, 892, 2387, 2542, 2921, 3708, 3993
AclWI GGATC 7 cut(s) 594, 947, 1223, 2521, 3128, 3169, 3906
AcoI YGGCCR 5 cut(s) 507, 1654, 2158, 2269, 3307
AcsI RAATTY 4 cut(s) 1288, 2532, 3442, 3936
AcuI CTGAAG 5 cut(s) 1164, 1710, 2039, 2048, 2765
AcyI GRCGYC 1 cut(s) 1030
AdeI CACNNNGTG 1 cut(s) 3220
AfaI GTAC 8 cut(s) 18, 81, 253, 379, 2014, 2250, 3544, 4097
AfeI AGCGCT 2 cut(s) 728, 3831
AfiI CCNNNNNNNGG 9 cut(s) 100, 132, 193, 806, 1919, 2934, 3184, 3292, 3644
AflII CTTAAG 1 cut(s) 2330
AflIII ACRYGT 1 cut(s) 744
AgeI ACCGGT 1 cut(s) 700
AhdI GACNNNNNGTC 1 cut(s) 1378
AjnI CCWGG 9 cut(s) 23, 292, 1363, 1651, 1916, 2007, 3178, 3291, 4080
AleI CACNNNNGTG 2 cut(s) 1828, 2490
Alw21I GWGCWC 2 cut(s) 2497, 3625
Alw26I GTCTC 9 cut(s) 76, 115, 1386, 1500, 1982, 2676, 2908, 3193, 3685
AlwI GGATC 7 cut(s) 594, 947, 1223, 2521, 3128, 3169, 3906
Ama87I CYCGRG 1 cut(s) 3087
Aor51HI AGCGCT 2 cut(s) 728, 3831
AoxI GGCC 6 cut(s) 507, 1541, 1654, 2158, 2269, 3307
ApoI RAATTY 4 cut(s) 1288, 2532, 3442, 3936
ArsI GACNNNNNNTTYG 2 cut(s) 518, 550
AseI ATTAAT 2 cut(s) 2169, 2645
AsiGI ACCGGT 1 cut(s) 700
Asp700I GAANNNNTTC 1 cut(s) 3936
AspLEI GCGC 5 cut(s) 729, 770, 2367, 3337, 3832
AspS9I GGNCC 9 cut(s) 128, 145, 461, 792, 1367, 1936, 2263, 3630, 3990
AvaI CYCGRG 1 cut(s) 3087
AvaII GGWCC 9 cut(s) 128, 145, 461, 792, 1367, 1936, 2263, 3630, 3990
BaeGI GKGCMC 1 cut(s) 3329
BalI TGGCCA 2 cut(s) 2160, 3309
BanI GGYRCC 1 cut(s) 3324
BarI GAAGNNNNNNTAC 2 cut(s) 3046, 3078
BbsI GAAGAC 2 cut(s) 219, 1454
Bbv12I GWGCWC 2 cut(s) 2497, 3625
BccI CCATC 7 cut(s) 133, 191, 783, 919, 2631, 3094, 3662
BceAI ACGGC 2 cut(s) 1641, 3270
BcgI CGANNNNNNTGC 2 cut(s) 752, 786
BciT130I CCWGG 9 cut(s) 25, 294, 1365, 1653, 1918, 2009, 3180, 3293, 4082
BciVI GTATCC 3 cut(s) 2014, 2917, 3577
BclI TGATCA 2 cut(s) 2355, 2836
BcoDI GTCTC 9 cut(s) 76, 115, 1386, 1500, 1982, 2676, 2908, 3193, 3685
BfmI CTRYAG 7 cut(s) 1233, 1527, 1545, 1896, 3891, 3966, 4091
BfoI RGCGCY 2 cut(s) 730, 3833
BfrI CTTAAG 1 cut(s) 2330
BfuAI ACCTGC 3 cut(s) 156, 843, 2791
BfuI GTATCC 3 cut(s) 2014, 2917, 3577
Bme1390I CCNGG 9 cut(s) 25, 294, 1365, 1653, 1918, 2009, 3180, 3293, 4082
Bme18I GGWCC 9 cut(s) 128, 145, 461, 792, 1367, 1936, 2263, 3630, 3990
BmeRI GACNNNNNGTC 1 cut(s) 1378
BmeT110I CYCGRG 1 cut(s) 3087
BmgT120I GGNCC 9 cut(s) 128, 145, 461, 792, 1367, 1936, 2263, 3630, 3990
BmiI GGNNCC 6 cut(s) 1937, 1938, 2815, 3326, 3375, 3632
BmrFI CCNGG 9 cut(s) 25, 294, 1365, 1653, 1918, 2009, 3180, 3293, 4082
BpiI GAAGAC 2 cut(s) 219, 1454
BplI GAGNNNNNCTC 2 cut(s) 2888, 2920
BpmI CTGGAG 5 cut(s) 324, 542, 1900, 3836, 4053
BpuEI CTTGAG 7 cut(s) 707, 2025, 2403, 2666, 2952, 3434, 4047
Bsa29I ATCGAT 1 cut(s) 3600
BsaBI GATNNNNATC 4 cut(s) 516, 669, 2523, 4104
BsaHI GRCGYC 1 cut(s) 1030
BsaI GGTCTC 3 cut(s) 76, 1982, 3193
BsaWI WCCGGW 2 cut(s) 455, 700
BsaXI ACNNNNNCTCC 4 cut(s) 634, 664, 2842, 2872
Bsc4I CCNNNNNNNGG 9 cut(s) 100, 132, 193, 806, 1919, 2934, 3184, 3292, 3644
Bse118I RCCGGY 3 cut(s) 416, 700, 2878
Bse1I ACTGG 2 cut(s) 45, 3265
Bse3DI GCAATG 2 cut(s) 1564, 3738
Bse8I GATNNNNATC 4 cut(s) 516, 669, 2523, 4104
BseBI CCWGG 9 cut(s) 25, 294, 1365, 1653, 1918, 2009, 3180, 3293, 4082
BseCI ATCGAT 1 cut(s) 3600
BseJI GATNNNNATC 4 cut(s) 516, 669, 2523, 4104
BseLI CCNNNNNNNGG 9 cut(s) 100, 132, 193, 806, 1919, 2934, 3184, 3292, 3644
BseMI GCAATG 2 cut(s) 1564, 3738
BseMII CTCAG 7 cut(s) 126, 1209, 1464, 2369, 3016, 3780, 3882
BseNI ACTGG 2 cut(s) 45, 3265
BseRI GAGGAG 5 cut(s) 1620, 2806, 2850, 3112, 3345
BseSI GKGCMC 1 cut(s) 3329
BseYI CCCAGC 2 cut(s) 1165, 3832
BsgI GTGCAG 3 cut(s) 246, 462, 681
Bsh1236I CGCG 1 cut(s) 490
Bsh1285I CGRYCG 1 cut(s) 3025
BshFI GGCC 6 cut(s) 509, 1543, 1656, 2160, 2271, 3309
BshNI GGYRCC 1 cut(s) 3324
BshTI ACCGGT 1 cut(s) 700
BshVI ATCGAT 1 cut(s) 3600
BsiEI CGRYCG 1 cut(s) 3025
BsiHKAI GWGCWC 2 cut(s) 2497, 3625
BsiHKCI CYCGRG 1 cut(s) 3087
BsiSI CCGG 5 cut(s) 126, 417, 456, 701, 2879
BslFI GGGAC 4 cut(s) 380, 1949, 1969, 3616
BslI CCNNNNNNNGG 9 cut(s) 100, 132, 193, 806, 1919, 2934, 3184, 3292, 3644
BsmAI GTCTC 9 cut(s) 76, 115, 1386, 1500, 1982, 2676, 2908, 3193, 3685
BsmFI GGGAC 4 cut(s) 380, 1949, 1969, 3616
BsmI GAATGC 1 cut(s) 2117
BsnI GGCC 6 cut(s) 509, 1543, 1656, 2160, 2271, 3309
Bso31I GGTCTC 3 cut(s) 76, 1982, 3193
BsoBI CYCGRG 1 cut(s) 3087
Bsp1286I GDGCHC 3 cut(s) 2497, 3329, 3625
Bsp19I CCATGG 2 cut(s) 404, 2266
BspACI CCGC 7 cut(s) 490, 892, 2387, 2542, 2921, 3708, 3993
BspANI GGCC 6 cut(s) 509, 1543, 1656, 2160, 2271, 3309
BspCNI CTCAG 7 cut(s) 125, 1210, 1465, 2370, 3015, 3781, 3881
BspDI ATCGAT 1 cut(s) 3600
BspFNI CGCG 1 cut(s) 490
BspHI TCATGA 1 cut(s) 3901
BspLI GGNNCC 6 cut(s) 1937, 1938, 2815, 3326, 3375, 3632
BspMAI CTGCAG 3 cut(s) 1237, 1531, 3895
BspMI ACCTGC 3 cut(s) 156, 843, 2791
BspPI GGATC 7 cut(s) 594, 947, 1223, 2521, 3128, 3169, 3906
BspQI GCTCTTC 1 cut(s) 2174
BspT107I GGYRCC 1 cut(s) 3324
BspTI CTTAAG 1 cut(s) 2330
BspTNI GGTCTC 3 cut(s) 76, 1982, 3193
BsrDI GCAATG 2 cut(s) 1564, 3738
BsrFI RCCGGY 3 cut(s) 416, 700, 2878
BsrI ACTGG 2 cut(s) 45, 3265
BssAI RCCGGY 3 cut(s) 416, 700, 2878
BssNI GRCGYC 1 cut(s) 1030
BssT1I CCWWGG 5 cut(s) 66, 131, 404, 504, 2266
Bst2UI CCWGG 9 cut(s) 25, 294, 1365, 1653, 1918, 2009, 3180, 3293, 4082
Bst4CI ACNGT 8 cut(s) 32, 1376, 1843, 1864, 2253, 2776, 2854, 4095
Bst6I CTCTTC 5 cut(s) 987, 1638, 2174, 2911, 3555
BstACI GRCGYC 1 cut(s) 1030
BstAFI CTTAAG 1 cut(s) 2330
BstAPI GCANNNNNTGC 4 cut(s) 913, 1241, 1253, 3749
BstDSI CCRYGG 3 cut(s) 404, 467, 2266
BstFNI CGCG 1 cut(s) 490
BstH2I RGCGCY 2 cut(s) 730, 3833
BstHHI GCGC 5 cut(s) 729, 770, 2367, 3337, 3832
BstMAI GTCTC 9 cut(s) 76, 115, 1386, 1500, 1982, 2676, 2908, 3193, 3685
BstMCI CGRYCG 1 cut(s) 3025
BstNI CCWGG 9 cut(s) 25, 294, 1365, 1653, 1918, 2009, 3180, 3293, 4082
BstNSI RCATGY 3 cut(s) 748, 2153, 2606
BstSCI CCNGG 9 cut(s) 23, 292, 1363, 1651, 1916, 2007, 3178, 3291, 4080
BstSFI CTRYAG 7 cut(s) 1233, 1527, 1545, 1896, 3891, 3966, 4091
BstSLI GKGCMC 1 cut(s) 3329
BstUI CGCG 1 cut(s) 490
BstV2I GAAGAC 2 cut(s) 219, 1454
BstX2I RGATCY 1 cut(s) 3174
BstXI CCANNNNNNTGG 1 cut(s) 682
BstYI RGATCY 1 cut(s) 3174
Bsu15I ATCGAT 1 cut(s) 3600
BsuI GTATCC 3 cut(s) 2014, 2917, 3577
BsuRI GGCC 6 cut(s) 509, 1543, 1656, 2160, 2271, 3309
BsuTUI ATCGAT 1 cut(s) 3600
BtgI CCRYGG 3 cut(s) 404, 467, 2266
BtgZI GCGATG 1 cut(s) 494
BtsI GCAGTG 3 cut(s) 2191, 2418, 3882
BveI ACCTGC 3 cut(s) 156, 843, 2791
CciI TCATGA 1 cut(s) 3901
CfoI GCGC 5 cut(s) 729, 770, 2367, 3337, 3832
Cfr10I RCCGGY 3 cut(s) 416, 700, 2878
Cfr13I GGNCC 9 cut(s) 128, 145, 461, 792, 1367, 1936, 2263, 3630, 3990
ClaI ATCGAT 1 cut(s) 3600
CseI GACGC 4 cut(s) 496, 1019, 1086, 1116
CsiI ACCWGGT 1 cut(s) 3291
Csp6I GTAC 8 cut(s) 17, 80, 252, 378, 2013, 2249, 3543, 4096
CspAI ACCGGT 1 cut(s) 700
CspCI CAANNNNNGTGG 4 cut(s) 439, 474, 2471, 2506
CviQI GTAC 8 cut(s) 17, 80, 252, 378, 2013, 2249, 3543, 4096
DraI TTTAAA 1 cut(s) 1452
DraIII CACNNNGTG 1 cut(s) 3220
DrdI GACNNNNNNGTC 1 cut(s) 3769
DriI GACNNNNNGTC 1 cut(s) 1378
DseDI GACNNNNNNGTC 1 cut(s) 3769
EaeI YGGCCR 5 cut(s) 507, 1654, 2158, 2269, 3307
Eam1104I CTCTTC 5 cut(s) 987, 1638, 2174, 2911, 3555
Eam1105I GACNNNNNGTC 1 cut(s) 1378
EarI CTCTTC 5 cut(s) 987, 1638, 2174, 2911, 3555
Eco130I CCWWGG 5 cut(s) 66, 131, 404, 504, 2266
Eco147I AGGCCT 1 cut(s) 1543
Eco31I GGTCTC 3 cut(s) 76, 1982, 3193
Eco32I GATATC 2 cut(s) 1665, 2276
Eco47I GGWCC 9 cut(s) 128, 145, 461, 792, 1367, 1936, 2263, 3630, 3990
Eco47III AGCGCT 2 cut(s) 728, 3831
Eco57I CTGAAG 5 cut(s) 1164, 1710, 2039, 2048, 2765
Eco88I CYCGRG 1 cut(s) 3087
EcoO109I RGGNCCY 2 cut(s) 145, 1936
EcoRI GAATTC 1 cut(s) 1288
EcoRII CCWGG 9 cut(s) 23, 292, 1363, 1651, 1916, 2007, 3178, 3291, 4080
EcoRV GATATC 2 cut(s) 1665, 2276
EcoT14I CCWWGG 5 cut(s) 66, 131, 404, 504, 2266
EcoT22I ATGCAT 4 cut(s) 909, 1350, 1710, 2749
ErhI CCWWGG 5 cut(s) 66, 131, 404, 504, 2266
FalI AAGNNNNNCTT 2 cut(s) 3552, 3584
FaqI GGGAC 4 cut(s) 380, 1949, 1969, 3616
FauI CCCGC 1 cut(s) 2380
FauNDI CATATG 1 cut(s) 4047
FbaI TGATCA 2 cut(s) 2355, 2836
FspI TGCGCA 1 cut(s) 3336
GlaI GCGC 5 cut(s) 728, 769, 2366, 3336, 3831
GsaI CCCAGC 2 cut(s) 1169, 3836
GsuI CTGGAG 5 cut(s) 324, 542, 1900, 3836, 4053
HaeII RGCGCY 2 cut(s) 730, 3833
HaeIII GGCC 6 cut(s) 509, 1543, 1656, 2160, 2271, 3309
HapII CCGG 5 cut(s) 126, 417, 456, 701, 2879
HgaI GACGC 4 cut(s) 496, 1019, 1086, 1116
HhaI GCGC 5 cut(s) 729, 770, 2367, 3337, 3832
Hin1I GRCGYC 1 cut(s) 1030
Hin6I GCGC 5 cut(s) 727, 768, 2365, 3335, 3830
HinP1I GCGC 5 cut(s) 727, 768, 2365, 3335, 3830
HincII GTYRAC 2 cut(s) 46, 91
HindII GTYRAC 2 cut(s) 46, 91
HindIII AAGCTT 2 cut(s) 1020, 3038
HpaI GTTAAC 1 cut(s) 46
HpaII CCGG 5 cut(s) 126, 417, 456, 701, 2879
Hpy166II GTNNAC 9 cut(s) 46, 91, 464, 570, 812, 1785, 1892, 2015, 2876
Hpy8I GTNNAC 9 cut(s) 46, 91, 464, 570, 812, 1785, 1892, 2015, 2876
HpyCH4III ACNGT 8 cut(s) 32, 1376, 1843, 1864, 2253, 2776, 2854, 4095
HpyCH4IV ACGT 3 cut(s) 82, 2432, 2956
HpySE526I ACGT 3 cut(s) 82, 2432, 2956
Hsp92I GRCGYC 1 cut(s) 1030
HspAI GCGC 5 cut(s) 727, 768, 2365, 3335, 3830
KflI GGGWCCC 1 cut(s) 1936
KroI GCCGGC 1 cut(s) 416
KroNI GCCGGC 1 cut(s) 418
Ksp22I TGATCA 2 cut(s) 2355, 2836
KspAI GTTAAC 1 cut(s) 46
LguI GCTCTTC 1 cut(s) 2174
LmnI GCTCC 6 cut(s) 1309, 1719, 2819, 3358, 3817, 4072
MabI ACCWGGT 1 cut(s) 3291
MaeII ACGT 3 cut(s) 82, 2432, 2956
MaeIII GTNAC 9 cut(s) 1881, 3156, 3212, 3222, 3254, 3287, 3408, 3760, 3886
MfeI CAATTG 1 cut(s) 1410
MflI RGATCY 1 cut(s) 3174
MhlI GDGCHC 3 cut(s) 2497, 3329, 3625
MlsI TGGCCA 2 cut(s) 2160, 3309
MluNI TGGCCA 2 cut(s) 2160, 3309
MlyI GAGTC 2 cut(s) 714, 2044
MmeI TCCRAC 5 cut(s) 564, 1741, 2453, 2873, 2915
Mox20I TGGCCA 2 cut(s) 2160, 3309
Mph1103I ATGCAT 4 cut(s) 909, 1350, 1710, 2749
MroNI GCCGGC 1 cut(s) 416
MroXI GAANNNNTTC 1 cut(s) 3936
MscI TGGCCA 2 cut(s) 2160, 3309
MslI CAYNNNNRTG 7 cut(s) 273, 680, 749, 1725, 1828, 2490, 4046
Msp20I TGGCCA 2 cut(s) 2160, 3309
MspA1I CMGCKG 4 cut(s) 446, 892, 1532, 2361
MspCI CTTAAG 1 cut(s) 2330
MspI CCGG 5 cut(s) 126, 417, 456, 701, 2879
MspR9I CCNGG 9 cut(s) 25, 294, 1365, 1653, 1918, 2009, 3180, 3293, 4082
MunI CAATTG 1 cut(s) 1410
Mva1269I GAATGC 1 cut(s) 2117
MvaI CCWGG 9 cut(s) 25, 294, 1365, 1653, 1918, 2009, 3180, 3293, 4082
MvnI CGCG 1 cut(s) 490
NaeI GCCGGC 1 cut(s) 418
NcoI CCATGG 2 cut(s) 404, 2266
NdeI CATATG 1 cut(s) 4047
NgoMIV GCCGGC 1 cut(s) 416
NlaIV GGNNCC 6 cut(s) 1937, 1938, 2815, 3326, 3375, 3632
NmuCI GTSAC 5 cut(s) 3156, 3212, 3287, 3760, 3886
NsbI TGCGCA 1 cut(s) 3336
NsiI ATGCAT 4 cut(s) 909, 1350, 1710, 2749
NspI RCATGY 3 cut(s) 748, 2153, 2606
OliI CACNNNNGTG 2 cut(s) 1828, 2490
PaeI GCATGC 2 cut(s) 2153, 2606
PagI TCATGA 1 cut(s) 3901
PasI CCCWGGG 2 cut(s) 2008, 3179
PceI AGGCCT 1 cut(s) 1543
PciI ACATGT 1 cut(s) 744
PciSI GCTCTTC 1 cut(s) 2174
PctI GAATGC 1 cut(s) 2117
PdiI GCCGGC 1 cut(s) 418
PdmI GAANNNNTTC 1 cut(s) 3936
PfeI GAWTC 9 cut(s) 315, 397, 1469, 2398, 2519, 2596, 2732, 2845, 4034
PflFI GACNNNGTC 1 cut(s) 1110
PflMI CCANNNNNTGG 1 cut(s) 2934
PfoI TCCNGGA 1 cut(s) 4080
PinAI ACCGGT 1 cut(s) 700
Ple19I CGATCG 1 cut(s) 3025
PleI GAGTC 2 cut(s) 714, 2043
PpsI GAGTC 2 cut(s) 714, 2043
PpuMI RGGWCCY 2 cut(s) 145, 1936
PscI ACATGT 1 cut(s) 744
PshBI ATTAAT 2 cut(s) 2169, 2645
Psp5II RGGWCCY 2 cut(s) 145, 1936
Psp6I CCWGG 9 cut(s) 23, 292, 1363, 1651, 1916, 2007, 3178, 3291, 4080
PspFI CCCAGC 2 cut(s) 1165, 3832
PspGI CCWGG 9 cut(s) 23, 292, 1363, 1651, 1916, 2007, 3178, 3291, 4080
PspN4I GGNNCC 6 cut(s) 1937, 1938, 2815, 3326, 3375, 3632
PspPI GGNCC 9 cut(s) 128, 145, 461, 792, 1367, 1936, 2263, 3630, 3990
PspPPI RGGWCCY 2 cut(s) 145, 1936
PsrI GAACNNNNNNTAC 2 cut(s) 3548, 3580
PstI CTGCAG 3 cut(s) 1237, 1531, 3895
PsuI RGATCY 1 cut(s) 3174
PsyI GACNNNGTC 1 cut(s) 1110
PvuI CGATCG 1 cut(s) 3025
PvuII CAGCTG 3 cut(s) 446, 1532, 2361
RsaI GTAC 8 cut(s) 18, 81, 253, 379, 2014, 2250, 3544, 4097
RsaNI GTAC 8 cut(s) 17, 80, 252, 378, 2013, 2249, 3543, 4096
RseI CAYNNNNRTG 7 cut(s) 273, 680, 749, 1725, 1828, 2490, 4046
SapI GCTCTTC 1 cut(s) 2174
Sau96I GGNCC 9 cut(s) 128, 145, 461, 792, 1367, 1936, 2263, 3630, 3990
SchI GAGTC 2 cut(s) 714, 2044
ScrFI CCNGG 9 cut(s) 25, 294, 1365, 1653, 1918, 2009, 3180, 3293, 4082
SduI GDGCHC 3 cut(s) 2497, 3329, 3625
SexAI ACCWGGT 1 cut(s) 3291
SfcI CTRYAG 7 cut(s) 1233, 1527, 1545, 1896, 3891, 3966, 4091
SinI GGWCC 9 cut(s) 128, 145, 461, 792, 1367, 1936, 2263, 3630, 3990
SmiMI CAYNNNNRTG 7 cut(s) 273, 680, 749, 1725, 1828, 2490, 4046
SmlI CTYRAG 8 cut(s) 722, 2040, 2330, 2418, 2681, 2967, 3413, 4062
SmoI CTYRAG 8 cut(s) 722, 2040, 2330, 2418, 2681, 2967, 3413, 4062
SphI GCATGC 2 cut(s) 2153, 2606
SseBI AGGCCT 1 cut(s) 1543
SsiI CCGC 7 cut(s) 490, 892, 2387, 2542, 2921, 3708, 3993
SspI AATATT 2 cut(s) 1207, 2893
StuI AGGCCT 1 cut(s) 1543
StyD4I CCNGG 9 cut(s) 23, 292, 1363, 1651, 1916, 2007, 3178, 3291, 4080
StyI CCWWGG 5 cut(s) 66, 131, 404, 504, 2266
TaaI ACNGT 8 cut(s) 32, 1376, 1843, 1864, 2253, 2776, 2854, 4095
TaiI ACGT 3 cut(s) 85, 2435, 2959
TaqI TCGA 3 cut(s) 34, 886, 3600
TaqII GACCGA 1 cut(s) 648
TatI WGTACW 1 cut(s) 3542
TfiI GAWTC 9 cut(s) 315, 397, 1469, 2398, 2519, 2596, 2732, 2845, 4034
TseFI GTSAC 5 cut(s) 3156, 3212, 3287, 3760, 3886
Tsp45I GTSAC 5 cut(s) 3156, 3212, 3287, 3760, 3886
TspGWI ACGGA 2 cut(s) 484, 1341
Tth111I GACNNNGTC 1 cut(s) 1110
Van91I CCANNNNNTGG 1 cut(s) 2934
Vha464I CTTAAG 1 cut(s) 2330
VpaK11BI GGWCC 9 cut(s) 128, 145, 461, 792, 1367, 1936, 2263, 3630, 3990
VspI ATTAAT 2 cut(s) 2169, 2645
XapI RAATTY 4 cut(s) 1288, 2532, 3442, 3936
XceI RCATGY 3 cut(s) 748, 2153, 2606
XcmI CCANNNNNNNNNTGG 1 cut(s) 3317
XmnI GAANNNNTTC 1 cut(s) 3936
Zsp2I ATGCAT 4 cut(s) 909, 1350, 1710, 2749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.