MD08G1172500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0014755
Physical Location & Seq
Forward (+)
20792408 .. 20793527
1120 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1172500.v1.1.491

Sequence Viewer

Length: 552 bp
ATGGCAAAACAAGAGTTGGAGAAAGAGGAGAAGAACAATGGCACAAGAGAGAGACCTAACAAGAAGGATGTGATCATCACTGTCTATGTGGAATCTCCGGCATCGTCTGATTCTCATCAGAAACCTGGTCCTGTCAAGAAGATTAACCCCAAGCCGAGCCCCGAAAAGCCTCATCAGACTGCGCAAACACGAATCTATGATCGCCGGGCTCAACTCCTTGCTTATTCTCAAGAGCTAAGAACAACTGGTGATGCTCAGAAAGTGCAGTGGACTAACAACGGTTCAAAGCCTAAGCCTAGGGTGGGAAACAAAAGGCTCATAACTCCAATAAGACACAGAAGAACAAAGAGCAGAGGGAGATATCAACGCTTAGTACCAGAAGTGGACGTATATTGCAATCCCAAATCGATCATAGGCAGGAACAAAAAGGCCATCGGATCAGAAACAAGTTCTAACTTTCTGAGGAAGCTAAGGTGTGTGCTGAGAGGAATTTCTGGTTTGCTCTGCAACAAAGGGTCCAAAGTTGCAGAGAGAAATTATCATGCTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

184

Amino Acids

20.84

Weight (kDa)

10.42

Isoelectric Point (pI)

41.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014755)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g39000
malus_domestica MD08G1172500.v1.1 MD15G1358100.v1.1
prunus_persica Prupe.1G505500_v2.0.a1
pyrus_communis pycom08g14730 pycom15g32040
rosa_laevigata RLG00000000610
rosa_multiflora Rmu_co7979250.1_g000001 Rmu_ssc0000392.1_g000008
rosa_roxburghii Rroxscaffold_3G00219370
rosa_rugosa Rorug07G0340900 Rorug07G0341000
rosa_samantha Rh7AG495700 Rh7BG467100 Rh7CG513200 Rh7DG480300
rosa_wichuraiana Rw7G041890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 183
AclWI GGATC 1 cut(s) 445
AcsI RAATTY 1 cut(s) 489
AfaI GTAC 1 cut(s) 375
AfiI CCNNNNNNNGG 1 cut(s) 302
AgsI TTSAA 1 cut(s) 285
AjnI CCWGG 1 cut(s) 124
AluBI AGCT 2 cut(s) 235, 469
AluI AGCT 2 cut(s) 235, 469
Alw26I GTCTC 1 cut(s) 46
AlwI GGATC 1 cut(s) 445
AoxI GGCC 1 cut(s) 429
ApoI RAATTY 1 cut(s) 489
AspA2I CCTAGG 1 cut(s) 296
AspLEI GCGC 1 cut(s) 184
AspS9I GGNCC 2 cut(s) 128, 516
AsuC2I CCSGG 1 cut(s) 206
AsuHPI GGTGA 1 cut(s) 260
AvaII GGWCC 2 cut(s) 128, 516
AvrII CCTAGG 1 cut(s) 296
BanII GRGCYC 2 cut(s) 161, 211
BccI CCATC 1 cut(s) 440
BciT130I CCWGG 1 cut(s) 126
BclI TGATCA 1 cut(s) 72
BcnI CCSGG 1 cut(s) 206
BcoDI GTCTC 1 cut(s) 46
BfaI CTAG 1 cut(s) 297
BlnI CCTAGG 1 cut(s) 296
Bme1390I CCNGG 2 cut(s) 126, 206
Bme18I GGWCC 2 cut(s) 128, 516
BmgT120I GGNCC 2 cut(s) 128, 516
BmiI GGNNCC 1 cut(s) 517
BmrFI CCNGG 2 cut(s) 126, 206
BmsI GCATC 2 cut(s) 110, 241
Bpu10I CCTNAGC 2 cut(s) 291, 470
BpuEI CTTGAG 1 cut(s) 213
BpuMI CCSGG 1 cut(s) 206
Bsa29I ATCGAT 1 cut(s) 407
BsaBI GATNNNNATC 1 cut(s) 114
BsaI GGTCTC 1 cut(s) 46
BsaJI CCNNGG 1 cut(s) 296
Bsc4I CCNNNNNNNGG 1 cut(s) 302
Bse1I ACTGG 1 cut(s) 250
Bse8I GATNNNNATC 1 cut(s) 114
BseBI CCWGG 1 cut(s) 126
BseCI ATCGAT 1 cut(s) 407
BseDI CCNNGG 1 cut(s) 296
BseGI GGATG 1 cut(s) 73
BseJI GATNNNNATC 1 cut(s) 114
BseLI CCNNNNNNNGG 1 cut(s) 302
BseMII CTCAG 3 cut(s) 269, 452, 473
BseNI ACTGG 1 cut(s) 250
BseRI GAGGAG 1 cut(s) 41
BsgI GTGCAG 1 cut(s) 284
BshFI GGCC 1 cut(s) 431
BshVI ATCGAT 1 cut(s) 407
BsiSI CCGG 2 cut(s) 98, 205
BslI CCNNNNNNNGG 1 cut(s) 302
BsmAI GTCTC 1 cut(s) 46
BsnI GGCC 1 cut(s) 431
Bso31I GGTCTC 1 cut(s) 46
Bsp1286I GDGCHC 2 cut(s) 161, 211
Bsp143I GATC 4 cut(s) 72, 199, 408, 437
BspANI GGCC 1 cut(s) 431
BspCNI CTCAG 3 cut(s) 268, 453, 474
BspDI ATCGAT 1 cut(s) 407
BspLI GGNNCC 1 cut(s) 517
BspPI GGATC 1 cut(s) 445
BspTNI GGTCTC 1 cut(s) 46
BsrI ACTGG 1 cut(s) 250
BssECI CCNNGG 1 cut(s) 296
BssMI GATC 4 cut(s) 72, 199, 408, 437
BssT1I CCWWGG 1 cut(s) 296
Bst2UI CCWGG 1 cut(s) 126
Bst4CI ACNGT 2 cut(s) 82, 281
BstDEI CTNAG 7 cut(s) 236, 255, 291, 370, 461, 470, 482
BstF5I GGATG 1 cut(s) 73
BstHHI GCGC 1 cut(s) 184
BstKTI GATC 4 cut(s) 75, 202, 411, 440
BstMAI GTCTC 1 cut(s) 46
BstMBI GATC 4 cut(s) 72, 199, 408, 437
BstNI CCWGG 1 cut(s) 126
BstSCI CCNGG 2 cut(s) 124, 204
Bsu15I ATCGAT 1 cut(s) 407
BsuRI GGCC 1 cut(s) 431
BsuTUI ATCGAT 1 cut(s) 407
BtsCI GGATG 1 cut(s) 73
BtsI GCAGTG 1 cut(s) 272
BtsIMutI CAGTG 2 cut(s) 78, 272
CfoI GCGC 1 cut(s) 184
Cfr13I GGNCC 2 cut(s) 128, 516
ClaI ATCGAT 1 cut(s) 407
CsiI ACCWGGT 1 cut(s) 124
Csp6I GTAC 1 cut(s) 374
CviAII CATG 1 cut(s) 542
CviQI GTAC 1 cut(s) 374
DdeI CTNAG 7 cut(s) 236, 255, 291, 370, 461, 470, 482
DpnI GATC 4 cut(s) 74, 201, 410, 439
DpnII GATC 4 cut(s) 72, 199, 408, 437
Eco130I CCWWGG 1 cut(s) 296
Eco24I GRGCYC 2 cut(s) 161, 211
Eco31I GGTCTC 1 cut(s) 46
Eco32I GATATC 1 cut(s) 362
Eco47I GGWCC 2 cut(s) 128, 516
EcoRII CCWGG 1 cut(s) 124
EcoRV GATATC 1 cut(s) 362
EcoT14I CCWWGG 1 cut(s) 296
EcoT38I GRGCYC 2 cut(s) 161, 211
ErhI CCWWGG 1 cut(s) 296
FaeI CATG 1 cut(s) 545
FaiI YATR 6 cut(s) 87, 198, 320, 391, 413, 543
FatI CATG 1 cut(s) 541
FbaI TGATCA 1 cut(s) 72
FokI GGATG 1 cut(s) 80
FriOI GRGCYC 2 cut(s) 161, 211
FspBI CTAG 1 cut(s) 297
FspI TGCGCA 1 cut(s) 183
GlaI GCGC 1 cut(s) 183
HaeIII GGCC 1 cut(s) 431
HapII CCGG 2 cut(s) 98, 205
HhaI GCGC 1 cut(s) 184
Hin1II CATG 1 cut(s) 545
Hin6I GCGC 1 cut(s) 182
HinP1I GCGC 1 cut(s) 182
HinfI GANTC 3 cut(s) 92, 110, 192
HpaII CCGG 2 cut(s) 98, 205
HphI GGTGA 1 cut(s) 260
Hpy166II GTNNAC 2 cut(s) 270, 385
Hpy188I TCNGA 7 cut(s) 109, 120, 177, 258, 437, 442, 462
Hpy188III TCNNGA 2 cut(s) 136, 230
Hpy8I GTNNAC 2 cut(s) 270, 385
HpyAV CCTTC 1 cut(s) 58
HpyCH4III ACNGT 2 cut(s) 82, 281
HpyCH4IV ACGT 1 cut(s) 387
HpyCH4V TGCA 4 cut(s) 265, 396, 507, 527
HpyF3I CTNAG 7 cut(s) 236, 255, 291, 370, 461, 470, 482
HpySE526I ACGT 1 cut(s) 387
Hsp92II CATG 1 cut(s) 545
HspAI GCGC 1 cut(s) 182
Ksp22I TGATCA 1 cut(s) 72
Kzo9I GATC 4 cut(s) 72, 199, 408, 437
LpnPI CCDG 9 cut(s) 111, 111, 138, 144, 218, 231, 390, 403, 480
LweI GCATC 2 cut(s) 110, 241
MabI ACCWGGT 1 cut(s) 124
MaeI CTAG 1 cut(s) 297
MaeII ACGT 1 cut(s) 387
MalI GATC 4 cut(s) 74, 201, 410, 439
MboI GATC 4 cut(s) 72, 199, 408, 437
MboII GAAGA 3 cut(s) 43, 151, 351
MhlI GDGCHC 2 cut(s) 161, 211
MluCI AATT 2 cut(s) 489, 535
MnlI CCTC 5 cut(s) 19, 180, 347, 456, 479
MseI TTAA 1 cut(s) 144
MspI CCGG 2 cut(s) 98, 205
MspR9I CCNGG 2 cut(s) 126, 206
MvaI CCWGG 1 cut(s) 126
NciI CCSGG 1 cut(s) 206
NdeII GATC 4 cut(s) 72, 199, 408, 437
NlaIII CATG 1 cut(s) 545
NlaIV GGNNCC 1 cut(s) 517
NmeAIII GCCGAG 1 cut(s) 180
NsbI TGCGCA 1 cut(s) 183
PfeI GAWTC 3 cut(s) 92, 110, 192
Psp6I CCWGG 1 cut(s) 124
PspGI CCWGG 1 cut(s) 124
PspN4I GGNNCC 1 cut(s) 517
PspPI GGNCC 2 cut(s) 128, 516
RsaI GTAC 1 cut(s) 375
RsaNI GTAC 1 cut(s) 374
SaqAI TTAA 1 cut(s) 144
Sau3AI GATC 4 cut(s) 72, 199, 408, 437
Sau96I GGNCC 2 cut(s) 128, 516
ScrFI CCNGG 2 cut(s) 126, 206
SduI GDGCHC 2 cut(s) 161, 211
SetI ASST 6 cut(s) 58, 127, 237, 390, 471, 476
SexAI ACCWGGT 1 cut(s) 124
SfaNI GCATC 2 cut(s) 110, 241
SinI GGWCC 2 cut(s) 128, 516
SmlI CTYRAG 1 cut(s) 228
SmoI CTYRAG 1 cut(s) 228
Sse9I AATT 2 cut(s) 489, 535
SspMI CTAG 1 cut(s) 297
StyD4I CCNGG 2 cut(s) 124, 204
StyI CCWWGG 1 cut(s) 296
TaaI ACNGT 2 cut(s) 82, 281
TaiI ACGT 1 cut(s) 390
TaqI TCGA 1 cut(s) 407
TasI AATT 2 cut(s) 489, 535
TfiI GAWTC 3 cut(s) 92, 110, 192
Tru1I TTAA 1 cut(s) 144
Tru9I TTAA 1 cut(s) 144
TscAI CASTG 2 cut(s) 85, 272
TspRI CASTG 2 cut(s) 85, 272
VpaK11BI GGWCC 2 cut(s) 128, 516
XapI RAATTY 1 cut(s) 489
XmaJI CCTAGG 1 cut(s) 296
XspI CTAG 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.