MD08G1177300.v1.1
ERF Family

Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin- like GTPase superfamily. TrmE GTPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0012258
Physical Location & Seq
Reverse (-)
21623737 .. 21630000
6264 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1177300.v1.1.491

Sequence Viewer

Length: 1668 bp
ATGGCTCTGCTCTCAGCTTTCCGCTTCCGCCACTTACCGCCACTGTTCGCCGTAGCTTCCACTTCACTTCCCCTTCTCTCCCACCTCACTCTCAATAGTCTCAAACTCCACCCTCGCTCCCCGCTCTATTTACCCTCTTGCCACTCCTCTACTTTCACCAACTCTTCCCCGGACAAGACCCAGTCGCAGACCCAGACGCTGCTTCTCAGCAGAGACGAGCGAGCACTGAGTGGGTCCCAGCATCAGGAGAGCACGAGCACAATTGCGGCAATCGTGACGTCACTTGGAGGCCCACCCGCTGCGGTTGGGATCGTACGGTTGTCGGGGCCGTCTGCCGTGGCCATTGTCGGCCGCGTCTTCCGTCCGAGCAAGAAGAAGACGAAGAGGAAGAGCACGTGGCGACCCACCAGCCATGTGGTGGAGTACGGCGTCGTTTCGGACCCCAACGGCAATGTCGTCGACGAGGTTTTGGTTGTGCCCATGTTGGCGCCAAGGTCTTACACGCGGGAAGATGTCGTGGAACTCCAGTGCCATGGGACCCAAGTGTGCCTCAATCGTGTTCTCAGAGCTTGTGTTGAAGCTGGAGCAAGGCTCGCCGAACCAGGCGAGTTTACTCTTCGCGCCTTTCTCAATGGCCGGTTGGACCTTACGCAAGCTGAAAATGTTGACAAACTCATTTCAGCCAAGTCTGTGGCTGCAGCGGATGCGGCACTGGCTGCTATTCAGGGTGGCTTTGCTTCCATGGTTAAATCTATAAGAGCTCAGTGCATTGAGCTGCTTACGGAAATTGAAGCCCGTTTGGATTTTGATGATGAGATGCCACCGCTTGATACAAATGAAATTGTGAATAGAATAAATTCCATGTCGCAAGATGTTGAGAGTGCTCTGGAGACAGCCAATTATGACCAACTTCTGCAATCTGGATTACAGATAGCAATTCTGGGCCGTCCAAATGTTGGGAAGTCAAGTCTCCTCAATGCTTGGAGCAAATCTGAGAGAGCAATAGTTACAGAAATTGCTGGGACCACTCGAGATGTGGTTGAAGCCAGTATCACAGTTCATGGCATTCCTGTAACCCTTCTCGATACAGCGGGCATTAGGGAAACCAATGACATTGTGGAGAAAATTGGTGTGGAGAGGTCTGAAGCAGTTGCTATGGGTGCTGATGTTATTATAATGGCAGTAAGTGCTGTTGATGGCTGGACTCCAGAAGACACTGAACTTTTAAATAGAATACAATCTAATAAGAAATCTACTGGATCCTCCACCCCCATGATCCTTGTAATTAACAAAATCGACTGTGTTCCATCTGATTGCATGGAGGGGATTGGAAAGGATATCGCTTCGTTCAGTAAACATGTTTTGACATCTGCTGTCACTGGTCAAGGAATCCAGGGTTTAGAGACGGCAATATTAGAGATTGTTGGTCTTGACAAAATTTCTGTGGGGAACCGCAGATGGACTGTAAACCAGAGACAATGCGAGCAGCTATTTCGGACCAAGGAGGCGTTTGTGAGTTTGAAATCTTCAATAGAAGAGGAAATGCCTTTCGACTTCTGGACAATTGATTTGAGGGATGCGGCAATGGCCCTAGGGCATATAAGCGGAGAAGACATTTCCGAGGAGGTTCTGTCCAACATTTTTGGCAACTTCTGTATTGGTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MMR_HSR1 MnmE_helical TrmE_N

Protein Analysis

556

Amino Acids

59.96

Weight (kDa)

5.46

Isoelectric Point (pI)

40.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TrmE_N PF10396 87 - 213 8.7e-38 GTP-binding protein TrmE N-terminus
MnmE_helical PF12631 216 - 552 7.2e-48 MnmE helical domain
MMR_HSR1 PF01926 310 - 431 4.9e-25 50S ribosome-binding GTPase
FeoB_N PF02421 310 - 468 1.1e-08 Ferrous iron transport protein B
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012258)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78010
fragaria_vesca FvH4_5g38010 FvH4_5g38010
malus_domestica MD08G1177300.v1.1 MD15G1355000.v1.1
prunus_persica Prupe.1G511900_v2.0.a1
pyrus_communis pycom08g15220
rosa_chinensis RchiOBHm_Chr7g0239951
rosa_laevigata RLG00000000715
rosa_multiflora Rmu_sc0000540.1_g000022 Rmu_sc0006825.1_g000001 Rmu_sc0035965.1_g000001
rosa_roxburghii Rroxscaffold_3G00220600
rosa_rugosa Rorug07G0331400
rosa_samantha Rh7AG485100 Rh7BG458100 Rh7CG502400 Rh7DG470700
rosa_wichuraiana Rw7G040690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1175
AatII GACGTC 1 cut(s) 281
AccB1I GGYRCC 1 cut(s) 487
AccB7I CCANNNNNTGG 2 cut(s) 418, 956
AccBSI CCGCTC 1 cut(s) 124
AccI GTMKAC 1 cut(s) 459
AccII CGCG 3 cut(s) 354, 505, 621
AclWI GGATC 4 cut(s) 317, 1254, 1267, 1270
AcoI YGGCCR 3 cut(s) 339, 349, 634
AcsI RAATTY 2 cut(s) 856, 1437
AcuI CTGAAG 1 cut(s) 1164
AcvI CACGTG 1 cut(s) 396
AcyI GRCGYC 3 cut(s) 278, 429, 488
AdeI CACNNNGTG 1 cut(s) 230
AfaI GTAC 2 cut(s) 315, 425
AfiI CCNNNNNNNGG 3 cut(s) 244, 418, 956
AflIII ACRYGT 1 cut(s) 1357
AgsI TTSAA 5 cut(s) 578, 791, 1043, 1522, 1530
AjnI CCWGG 2 cut(s) 601, 1392
AluBI AGCT 8 cut(s) 17, 56, 569, 581, 656, 761, 775, 1489
AluI AGCT 8 cut(s) 17, 56, 569, 581, 656, 761, 775, 1489
Alw21I GWGCWC 6 cut(s) 226, 254, 260, 395, 763, 886
Alw26I GTCTC 6 cut(s) 104, 207, 884, 974, 1397, 1468
AlwI GGATC 4 cut(s) 317, 1254, 1267, 1270
AlwNI CAGNNNCTG 1 cut(s) 199
Ama87I CYCGRG 1 cut(s) 1029
AoxI GGCC 7 cut(s) 289, 326, 339, 349, 634, 943, 1587
ApeKI GCWGC 7 cut(s) 199, 299, 695, 698, 716, 775, 1486
ApoI RAATTY 2 cut(s) 856, 1437
ArsI GACNNNNNNTTYG 2 cut(s) 1552, 1584
Asp700I GAANNNNTTC 1 cut(s) 856
AspA2I CCTAGG 1 cut(s) 1591
AspLEI GCGC 2 cut(s) 490, 623
AsuC2I CCSGG 1 cut(s) 170
AsuHPI GGTGA 1 cut(s) 148
AvaI CYCGRG 1 cut(s) 1029
AvaII GGWCC 6 cut(s) 234, 439, 537, 643, 1023, 1497
AvrII CCTAGG 1 cut(s) 1591
BaeGI GKGCMC 1 cut(s) 480
BalI TGGCCA 1 cut(s) 341
BamHI GGATCC 1 cut(s) 1259
BanI GGYRCC 1 cut(s) 487
BanII GRGCYC 1 cut(s) 763
BauI CACGAG 1 cut(s) 253
BbrPI CACGTG 1 cut(s) 396
BbsI GAAGAC 4 cut(s) 349, 383, 1218, 1617
Bbv12I GWGCWC 6 cut(s) 226, 254, 260, 395, 763, 886
BbvI GCAGC 7 cut(s) 186, 286, 682, 703, 710, 762, 1498
BccI CCATC 3 cut(s) 1190, 1315, 1452
BceAI ACGGC 7 cut(s) 35, 313, 320, 442, 463, 930, 1422
BcgI CGANNNNNNTGC 4 cut(s) 439, 473, 1475, 1509
BciT130I CCWGG 2 cut(s) 603, 1394
BcnI CCSGG 1 cut(s) 170
BcoDI GTCTC 6 cut(s) 104, 207, 884, 974, 1397, 1468
BfaI CTAG 1 cut(s) 1592
BfmI CTRYAG 1 cut(s) 696
BfoI RGCGCY 1 cut(s) 491
BlnI CCTAGG 1 cut(s) 1591
Bme1390I CCNGG 3 cut(s) 170, 603, 1394
Bme18I GGWCC 6 cut(s) 234, 439, 537, 643, 1023, 1497
BmeT110I CYCGRG 1 cut(s) 1029
BmrFI CCNGG 3 cut(s) 170, 603, 1394
BmrI ACTGGG 1 cut(s) 175
BmsI GCATC 4 cut(s) 250, 694, 807, 1567
BmuI ACTGGG 1 cut(s) 175
BpiI GAAGAC 4 cut(s) 349, 383, 1218, 1617
BplI GAGNNNNNCTC 2 cut(s) 576, 608
BpmI CTGGAG 4 cut(s) 509, 603, 908, 1191
BpuMI CCSGG 1 cut(s) 170
BsaAI YACGTR 1 cut(s) 396
BsaHI GRCGYC 3 cut(s) 278, 429, 488
BsaJI CCNNGG 9 cut(s) 168, 336, 491, 532, 741, 1393, 1500, 1591, 1620
BsaXI ACNNNNNCTCC 4 cut(s) 101, 131, 1616, 1646
Bsc4I CCNNNNNNNGG 3 cut(s) 244, 418, 956
Bse118I RCCGGY 1 cut(s) 636
Bse1I ACTGG 6 cut(s) 181, 526, 717, 1047, 1261, 1384
Bse3DI GCAATG 2 cut(s) 457, 1590
BseBI CCWGG 2 cut(s) 603, 1394
BseDI CCNNGG 9 cut(s) 168, 336, 491, 532, 741, 1393, 1500, 1591, 1620
BseGI GGATG 2 cut(s) 709, 1582
BseLI CCNNNNNNNGG 3 cut(s) 244, 418, 956
BseMI GCAATG 2 cut(s) 457, 1590
BseMII CTCAG 6 cut(s) 27, 218, 220, 577, 776, 984
BseNI ACTGG 6 cut(s) 181, 526, 717, 1047, 1261, 1384
BseRI GAGGAG 3 cut(s) 136, 962, 1637
BseSI GKGCMC 1 cut(s) 480
BseX3I CGGCCG 1 cut(s) 349
BseXI GCAGC 7 cut(s) 186, 286, 682, 703, 710, 762, 1498
BseYI CCCAGC 2 cut(s) 237, 1019
Bsh1236I CGCG 3 cut(s) 354, 505, 621
Bsh1285I CGRYCG 1 cut(s) 352
BshFI GGCC 7 cut(s) 291, 328, 341, 351, 636, 945, 1589
BshNI GGYRCC 1 cut(s) 487
BsiEI CGRYCG 1 cut(s) 352
BsiHKAI GWGCWC 6 cut(s) 226, 254, 260, 395, 763, 886
BsiHKCI CYCGRG 1 cut(s) 1029
BsiSI CCGG 2 cut(s) 170, 637
BsiWI CGTACG 1 cut(s) 313
BslFI GGGAC 3 cut(s) 220, 550, 1036
BslI CCNNNNNNNGG 3 cut(s) 244, 418, 956
BsmAI GTCTC 6 cut(s) 104, 207, 884, 974, 1397, 1468
BsmBI CGTCTC 2 cut(s) 207, 1397
BsmFI GGGAC 3 cut(s) 220, 550, 1036
BsmI GAATGC 1 cut(s) 1065
BsnI GGCC 7 cut(s) 291, 328, 341, 351, 636, 945, 1589
BsoBI CYCGRG 1 cut(s) 1029
Bsp1286I GDGCHC 7 cut(s) 226, 254, 260, 395, 480, 763, 886
Bsp143I GATC 3 cut(s) 309, 1259, 1275
Bsp19I CCATGG 2 cut(s) 532, 741
BspANI GGCC 7 cut(s) 291, 328, 341, 351, 636, 945, 1589
BspCNI CTCAG 6 cut(s) 26, 219, 219, 576, 775, 985
BspFNI CGCG 3 cut(s) 354, 505, 621
BspMAI CTGCAG 1 cut(s) 700
BspPI GGATC 4 cut(s) 317, 1254, 1267, 1270
BspQI GCTCTTC 1 cut(s) 383
BspT107I GGYRCC 1 cut(s) 487
BsrBI CCGCTC 1 cut(s) 124
BsrDI GCAATG 2 cut(s) 457, 1590
BsrFI RCCGGY 1 cut(s) 636
BsrI ACTGG 6 cut(s) 181, 526, 717, 1047, 1261, 1384
BssAI RCCGGY 1 cut(s) 636
BssECI CCNNGG 9 cut(s) 168, 336, 491, 532, 741, 1393, 1500, 1591, 1620
BssMI GATC 3 cut(s) 309, 1259, 1275
BssNI GRCGYC 3 cut(s) 278, 429, 488
BssSI CACGAG 1 cut(s) 253
BssT1I CCWWGG 5 cut(s) 491, 532, 741, 1500, 1591
Bst2BI CACGAG 1 cut(s) 253
Bst2UI CCWGG 2 cut(s) 603, 1394
Bst4CI ACNGT 5 cut(s) 45, 318, 1057, 1301, 1465
Bst6I CTCTTC 5 cut(s) 169, 377, 383, 621, 1530
BstACI GRCGYC 3 cut(s) 278, 429, 488
BstAPI GCANNNNNTGC 3 cut(s) 704, 716, 1187
BstBAI YACGTR 1 cut(s) 396
BstC8I GCNNGC 5 cut(s) 222, 594, 654, 1093, 1484
BstDEI CTNAG 6 cut(s) 13, 206, 227, 563, 762, 993
BstDSI CCRYGG 3 cut(s) 336, 532, 741
BstF5I GGATG 2 cut(s) 709, 1582
BstFNI CGCG 3 cut(s) 354, 505, 621
BstH2I RGCGCY 1 cut(s) 491
BstHHI GCGC 2 cut(s) 490, 623
BstKTI GATC 3 cut(s) 312, 1262, 1278
BstMAI GTCTC 6 cut(s) 104, 207, 884, 974, 1397, 1468
BstMBI GATC 3 cut(s) 309, 1259, 1275
BstMCI CGRYCG 1 cut(s) 352
BstMWI GCNNNNNNNGC 8 cut(s) 593, 704, 707, 713, 716, 1160, 1187, 1586
BstNI CCWGG 2 cut(s) 603, 1394
BstNSI RCATGY 1 cut(s) 1361
BstSCI CCNGG 3 cut(s) 168, 601, 1392
BstSFI CTRYAG 1 cut(s) 696
BstSLI GKGCMC 1 cut(s) 480
BstUI CGCG 3 cut(s) 354, 505, 621
BstV1I GCAGC 7 cut(s) 186, 286, 682, 703, 710, 762, 1498
BstV2I GAAGAC 4 cut(s) 349, 383, 1218, 1617
BstX2I RGATCY 1 cut(s) 1259
BstXI CCANNNNNNTGG 3 cut(s) 415, 533, 691
BstYI RGATCY 1 cut(s) 1259
BstZI CGGCCG 1 cut(s) 349
BsuRI GGCC 7 cut(s) 291, 328, 341, 351, 636, 945, 1589
BtgI CCRYGG 3 cut(s) 336, 532, 741
BtsCI GGATG 2 cut(s) 709, 1582
BtsIMutI CAGTG 7 cut(s) 41, 224, 533, 710, 770, 1215, 1377
Cac8I GCNNGC 5 cut(s) 222, 594, 654, 1093, 1484
CaiI CAGNNNCTG 1 cut(s) 199
CfoI GCGC 2 cut(s) 490, 623
Cfr10I RCCGGY 1 cut(s) 636
CseI GACGC 3 cut(s) 205, 343, 418
Csp6I GTAC 2 cut(s) 314, 424
CviAII CATG 9 cut(s) 413, 481, 533, 742, 862, 1061, 1273, 1318, 1358
CviQI GTAC 2 cut(s) 314, 424
DdeI CTNAG 6 cut(s) 13, 206, 227, 563, 762, 993
DinI GGCGCC 1 cut(s) 489
DpnI GATC 3 cut(s) 311, 1261, 1277
DpnII GATC 3 cut(s) 309, 1259, 1275
DraI TTTAAA 1 cut(s) 1227
DraIII CACNNNGTG 1 cut(s) 230
EaeI YGGCCR 3 cut(s) 339, 349, 634
EagI CGGCCG 1 cut(s) 349
Eam1104I CTCTTC 5 cut(s) 169, 377, 383, 621, 1530
EarI CTCTTC 5 cut(s) 169, 377, 383, 621, 1530
EciI GGCGGA 1 cut(s) 17
Ecl136II GAGCTC 1 cut(s) 761
EclXI CGGCCG 1 cut(s) 349
Eco130I CCWWGG 5 cut(s) 491, 532, 741, 1500, 1591
Eco24I GRGCYC 1 cut(s) 763
Eco32I GATATC 1 cut(s) 1339
Eco47I GGWCC 6 cut(s) 234, 439, 537, 643, 1023, 1497
Eco52I CGGCCG 1 cut(s) 349
Eco53kI GAGCTC 1 cut(s) 761
Eco57I CTGAAG 1 cut(s) 1164
Eco72I CACGTG 1 cut(s) 396
Eco88I CYCGRG 1 cut(s) 1029
EcoICRI GAGCTC 1 cut(s) 761
EcoO109I RGGNCCY 2 cut(s) 234, 537
EcoRII CCWGG 2 cut(s) 601, 1392
EcoRV GATATC 1 cut(s) 1339
EcoT14I CCWWGG 5 cut(s) 491, 532, 741, 1500, 1591
EcoT38I GRGCYC 1 cut(s) 763
EgeI GGCGCC 1 cut(s) 489
EheI GGCGCC 1 cut(s) 489
ErhI CCWWGG 5 cut(s) 491, 532, 741, 1500, 1591
Esp3I CGTCTC 2 cut(s) 207, 1397
FaeI CATG 9 cut(s) 416, 484, 536, 745, 865, 1064, 1276, 1321, 1361
FaqI GGGAC 3 cut(s) 220, 550, 1036
FatI CATG 9 cut(s) 412, 480, 532, 741, 861, 1060, 1272, 1317, 1357
FauI CCCGC 4 cut(s) 129, 304, 498, 1084
FblI GTMKAC 1 cut(s) 459
FokI GGATG 2 cut(s) 716, 1589
FriOI GRGCYC 1 cut(s) 763
FspBI CTAG 1 cut(s) 1592
GlaI GCGC 2 cut(s) 489, 622
GsaI CCCAGC 2 cut(s) 241, 1023
GsuI CTGGAG 4 cut(s) 509, 603, 908, 1191
HaeII RGCGCY 1 cut(s) 491
HaeIII GGCC 7 cut(s) 291, 328, 341, 351, 636, 945, 1589
HapII CCGG 2 cut(s) 170, 637
HgaI GACGC 3 cut(s) 205, 343, 418
HhaI GCGC 2 cut(s) 490, 623
Hin1I GRCGYC 3 cut(s) 278, 429, 488
Hin1II CATG 9 cut(s) 416, 484, 536, 745, 865, 1064, 1276, 1321, 1361
Hin6I GCGC 2 cut(s) 488, 621
HinP1I GCGC 2 cut(s) 488, 621
HincII GTYRAC 2 cut(s) 460, 667
HindII GTYRAC 2 cut(s) 460, 667
HinfI GANTC 2 cut(s) 1204, 1389
HpaII CCGG 2 cut(s) 170, 637
HphI GGTGA 1 cut(s) 148
Hpy166II GTNNAC 5 cut(s) 460, 612, 667, 1355, 1468
Hpy188I TCNGA 8 cut(s) 366, 439, 566, 994, 1144, 1312, 1497, 1621
Hpy188III TCNNGA 9 cut(s) 245, 274, 887, 921, 1031, 1082, 1208, 1430, 1558
Hpy8I GTNNAC 5 cut(s) 460, 612, 667, 1355, 1468
Hpy99I CGWCG 3 cut(s) 434, 461, 464
HpyAV CCTTC 2 cut(s) 83, 1088
HpyCH4III ACNGT 5 cut(s) 45, 318, 1057, 1301, 1465
HpyCH4IV ACGT 2 cut(s) 278, 395
HpyCH4V TGCA 4 cut(s) 698, 768, 916, 1317
HpyF10VI GCNNNNNNNGC 8 cut(s) 593, 704, 707, 713, 716, 1160, 1187, 1586
HpyF3I CTNAG 6 cut(s) 13, 206, 227, 563, 762, 993
HpySE526I ACGT 2 cut(s) 278, 395
Hsp92I GRCGYC 3 cut(s) 278, 429, 488
Hsp92II CATG 9 cut(s) 416, 484, 536, 745, 865, 1064, 1276, 1321, 1361
HspAI GCGC 2 cut(s) 488, 621
KasI GGCGCC 1 cut(s) 487
KflI GGGWCCC 2 cut(s) 234, 537
Kzo9I GATC 3 cut(s) 309, 1259, 1275
LguI GCTCTTC 1 cut(s) 383
LmnI GCTCC 3 cut(s) 122, 584, 984
Lsp1109I GCAGC 7 cut(s) 186, 286, 682, 703, 710, 762, 1498
LweI GCATC 4 cut(s) 250, 694, 807, 1567
MaeI CTAG 1 cut(s) 1592
MaeII ACGT 2 cut(s) 278, 395
MaeIII GTNAC 5 cut(s) 274, 279, 1006, 1072, 1375
MalI GATC 3 cut(s) 311, 1261, 1277
MbiI CCGCTC 1 cut(s) 124
MboI GATC 3 cut(s) 309, 1259, 1275
MfeI CAATTG 2 cut(s) 261, 1563
MflI RGATCY 1 cut(s) 1259
MhlI GDGCHC 7 cut(s) 226, 254, 260, 395, 480, 763, 886
MlsI TGGCCA 1 cut(s) 341
MluNI TGGCCA 1 cut(s) 341
Mly113I GGCGCC 1 cut(s) 488
MlyI GAGTC 1 cut(s) 1198
MmeI TCCRAC 2 cut(s) 621, 1659
Mox20I TGGCCA 1 cut(s) 341
MroXI GAANNNNTTC 1 cut(s) 856
MscI TGGCCA 1 cut(s) 341
MseI TTAA 3 cut(s) 747, 1226, 1287
MslI CAYNNNNRTG 1 cut(s) 1271
Msp20I TGGCCA 1 cut(s) 341
MspA1I CMGCKG 3 cut(s) 299, 701, 1091
MspI CCGG 2 cut(s) 170, 637
MspR9I CCNGG 3 cut(s) 170, 603, 1394
MunI CAATTG 2 cut(s) 261, 1563
Mva1269I GAATGC 1 cut(s) 1065
MvaI CCWGG 2 cut(s) 603, 1394
MvnI CGCG 3 cut(s) 354, 505, 621
MwoI GCNNNNNNNGC 8 cut(s) 593, 704, 707, 713, 716, 1160, 1187, 1586
NarI GGCGCC 1 cut(s) 488
NciI CCSGG 1 cut(s) 170
NcoI CCATGG 2 cut(s) 532, 741
NdeII GATC 3 cut(s) 309, 1259, 1275
NlaIII CATG 9 cut(s) 416, 484, 536, 745, 865, 1064, 1276, 1321, 1361
NmuCI GTSAC 3 cut(s) 274, 279, 1375
NspI RCATGY 1 cut(s) 1361
PaeR7I CTCGAG 1 cut(s) 1029
PciI ACATGT 1 cut(s) 1357
PciSI GCTCTTC 1 cut(s) 383
PcsI WCGNNNNNNNCGW 1 cut(s) 453
PctI GAATGC 1 cut(s) 1065
PdmI GAANNNNTTC 1 cut(s) 856
PfeI GAWTC 1 cut(s) 1389
Pfl23II CGTACG 1 cut(s) 313
PflFI GACNNNGTC 1 cut(s) 181
PflMI CCANNNNNTGG 2 cut(s) 418, 956
PleI GAGTC 1 cut(s) 1198
PluTI GGCGCC 1 cut(s) 491
PmaCI CACGTG 1 cut(s) 396
PmlI CACGTG 1 cut(s) 396
PpsI GAGTC 1 cut(s) 1198
Ppu21I YACGTR 1 cut(s) 396
PpuMI RGGWCCY 2 cut(s) 234, 537
PscI ACATGT 1 cut(s) 1357
PsiI TTATAA 1 cut(s) 1175
Psp124BI GAGCTC 1 cut(s) 763
Psp5II RGGWCCY 2 cut(s) 234, 537
Psp6I CCWGG 2 cut(s) 601, 1392
PspCI CACGTG 1 cut(s) 396
PspFI CCCAGC 2 cut(s) 237, 1019
PspGI CCWGG 2 cut(s) 601, 1392
PspLI CGTACG 1 cut(s) 313
PspPPI RGGWCCY 2 cut(s) 234, 537
PstI CTGCAG 1 cut(s) 700
PstNI CAGNNNCTG 1 cut(s) 199
PsuI RGATCY 1 cut(s) 1259
PsyI GACNNNGTC 1 cut(s) 181
RsaI GTAC 2 cut(s) 315, 425
RsaNI GTAC 2 cut(s) 314, 424
RseI CAYNNNNRTG 1 cut(s) 1271
SacI GAGCTC 1 cut(s) 763
SalI GTCGAC 1 cut(s) 458
SapI GCTCTTC 1 cut(s) 383
SaqAI TTAA 3 cut(s) 747, 1226, 1287
Sau3AI GATC 3 cut(s) 309, 1259, 1275
SchI GAGTC 1 cut(s) 1198
ScrFI CCNGG 3 cut(s) 170, 603, 1394
SduI GDGCHC 7 cut(s) 226, 254, 260, 395, 480, 763, 886
SfaNI GCATC 4 cut(s) 250, 694, 807, 1567
SfcI CTRYAG 1 cut(s) 696
SfoI GGCGCC 1 cut(s) 489
Sfr274I CTCGAG 1 cut(s) 1029
SgrDI CGTCGACG 1 cut(s) 458
SinI GGWCC 6 cut(s) 234, 439, 537, 643, 1023, 1497
SlaI CTCGAG 1 cut(s) 1029
SmiMI CAYNNNNRTG 1 cut(s) 1271
SmlI CTYRAG 1 cut(s) 1029
SmoI CTYRAG 1 cut(s) 1029
SspDI GGCGCC 1 cut(s) 487
SspI AATATT 1 cut(s) 1413
SspMI CTAG 1 cut(s) 1592
SstI GAGCTC 1 cut(s) 763
StyD4I CCNGG 3 cut(s) 168, 601, 1392
StyI CCWWGG 5 cut(s) 491, 532, 741, 1500, 1591
TaaI ACNGT 5 cut(s) 45, 318, 1057, 1301, 1465
TaiI ACGT 2 cut(s) 281, 398
TaqI TCGA 5 cut(s) 459, 1030, 1083, 1296, 1551
TauI GCSGC 4 cut(s) 269, 354, 710, 1583
TfiI GAWTC 1 cut(s) 1389
Tru1I TTAA 3 cut(s) 747, 1226, 1287
Tru9I TTAA 3 cut(s) 747, 1226, 1287
TscAI CASTG 7 cut(s) 48, 231, 533, 717, 770, 1222, 1384
TseFI GTSAC 3 cut(s) 274, 279, 1375
TseI GCWGC 7 cut(s) 199, 299, 695, 698, 716, 775, 1486
Tsp45I GTSAC 3 cut(s) 274, 279, 1375
TspDTI ATGAA 2 cut(s) 852, 1049
TspGWI ACGGA 2 cut(s) 350, 797
TspRI CASTG 7 cut(s) 48, 231, 533, 717, 770, 1222, 1384
Tth111I GACNNNGTC 1 cut(s) 181
Van91I CCANNNNNTGG 2 cut(s) 418, 956
VpaK11BI GGWCC 6 cut(s) 234, 439, 537, 643, 1023, 1497
XapI RAATTY 2 cut(s) 856, 1437
XceI RCATGY 1 cut(s) 1361
XcmI CCANNNNNNNNNTGG 4 cut(s) 412, 415, 1033, 1114
XhoI CTCGAG 1 cut(s) 1029
XmaJI CCTAGG 1 cut(s) 1591
XmiI GTMKAC 1 cut(s) 459
XmnI GAANNNNTTC 1 cut(s) 856
XspI CTAG 1 cut(s) 1592
ZraI GACGTC 1 cut(s) 279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.