pycom08g15220
ERF Family

Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin- like GTPase superfamily. TrmE GTPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0012258
Physical Location & Seq
Reverse (-)
14712791 .. 14713905
1115 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g15220.3

Sequence Viewer

Length: 771 bp
ATGGCTCTGCTCTCAGCTTTCCGCTTCCGCTACTTACCGCCACTGTTCGCCGTAGCTTCCACTTCACTTCCCCTTCTCTCCCACCTCACTCCCAATAGTCTCAAACTCCACCCTCGCTCCCCGCTCTATTTACCCCCTTGCCACTCCTCCACTTTCACCCACTCCTCCCCGGACAAGACCCAGACGCTGCTTCTCAGCCGAGACGAGCGAGTACTGAGTGGGTCCCAGCATCAGGAGAGCACGAGCACAATTGCGGCAATCGTGACGTCACTTGGAGGTCCACCCGCTGCGGTTGGGATCATACGGTTGTCGGGGCCGTCCGCCGTGGCCATTGTCGGCCGCGTCTTCCGTCCGAGCAAGAAGAAGACGAAGAGGAAGAGCACGTGGCGACCCACCAGCCATGTGGTGGAGTACGGCGTCGTTTCGGACCCCAACGGCAATGTCGTCGACGAGTACGGCGTCGTTTTGCTTGGGCTTAGGTTTTGGTTGTACCCATGTTGGCGCCAAGGTCTTACACGCGGGAAGATGTGGTGGAGCTCCAATGCCATGGAACCCAAGTGTGCCTCAATCGTGTTCTCAGAGCTTGTGTTGAAGCTGGAGCAAGGCTCGCCGAACCAGGCGAGTTTACTCTTCGCGCCTTTCTCAATGGCCGGTTGGACCTTACGCAAGCTGAAAATGTTGACAAACTCATTTCAGCCAAGTCTGTGGCTGCAGCGGATGCGGCACTGGCTGCTATTCAGGGGGGCTTTGCTTCCATGGTTAAATCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MMR_HSR1 MnmE_helical TrmE_N

Protein Analysis

257

Amino Acids

28.59

Weight (kDa)

10.58

Isoelectric Point (pI)

39.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012258)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78010
fragaria_vesca FvH4_5g38010 FvH4_5g38010
malus_domestica MD08G1177300.v1.1 MD15G1355000.v1.1
prunus_persica Prupe.1G511900_v2.0.a1
pyrus_communis pycom08g15220
rosa_chinensis RchiOBHm_Chr7g0239951
rosa_laevigata RLG00000000715
rosa_multiflora Rmu_sc0000540.1_g000022 Rmu_sc0006825.1_g000001 Rmu_sc0035965.1_g000001
rosa_roxburghii Rroxscaffold_3G00220600
rosa_rugosa Rorug07G0331400
rosa_samantha Rh7AG485100 Rh7BG458100 Rh7CG502400 Rh7DG470700
rosa_wichuraiana Rw7G040690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 269
AccB1I GGYRCC 1 cut(s) 501
AccB7I CCANNNNNTGG 1 cut(s) 406
AccBSI CCGCTC 1 cut(s) 124
AccI GTMKAC 1 cut(s) 447
AccII CGCG 3 cut(s) 342, 519, 635
AclWI GGATC 1 cut(s) 305
AcoI YGGCCR 3 cut(s) 327, 337, 648
AcvI CACGTG 1 cut(s) 384
AcyI GRCGYC 4 cut(s) 266, 417, 459, 502
AfaI GTAC 4 cut(s) 213, 413, 455, 491
AfiI CCNNNNNNNGG 2 cut(s) 232, 406
AgsI TTSAA 1 cut(s) 592
AjnI CCWGG 1 cut(s) 615
AluBI AGCT 6 cut(s) 17, 56, 537, 583, 595, 670
AluI AGCT 6 cut(s) 17, 56, 537, 583, 595, 670
Alw21I GWGCWC 4 cut(s) 242, 248, 383, 539
Alw26I GTCTC 2 cut(s) 104, 195
AlwI GGATC 1 cut(s) 305
AlwNI CAGNNNCTG 1 cut(s) 187
AoxI GGCC 4 cut(s) 314, 327, 337, 648
ApeKI GCWGC 5 cut(s) 187, 287, 709, 712, 730
AspLEI GCGC 2 cut(s) 504, 637
AspS9I GGNCC 5 cut(s) 222, 278, 314, 427, 657
AsuC2I CCSGG 1 cut(s) 170
AsuHPI GGTGA 1 cut(s) 148
AvaII GGWCC 4 cut(s) 222, 278, 427, 657
BalI TGGCCA 1 cut(s) 329
BanI GGYRCC 1 cut(s) 501
BanII GRGCYC 1 cut(s) 539
BauI CACGAG 1 cut(s) 241
BbrPI CACGTG 1 cut(s) 384
BbsI GAAGAC 2 cut(s) 337, 371
Bbv12I GWGCWC 4 cut(s) 242, 248, 383, 539
BbvI GCAGC 5 cut(s) 174, 274, 696, 717, 724
BceAI ACGGC 6 cut(s) 35, 301, 308, 430, 451, 472
BcgI CGANNNNNNTGC 2 cut(s) 427, 461
BciT130I CCWGG 1 cut(s) 617
BcnI CCSGG 1 cut(s) 170
BcoDI GTCTC 2 cut(s) 104, 195
BfmI CTRYAG 1 cut(s) 710
BfoI RGCGCY 1 cut(s) 505
BisI GCNGC 8 cut(s) 188, 255, 288, 340, 710, 713, 722, 731
BlsI GCNGC 8 cut(s) 189, 256, 289, 341, 711, 714, 723, 732
BmcAI AGTACT 1 cut(s) 213
Bme1390I CCNGG 2 cut(s) 170, 617
Bme18I GGWCC 4 cut(s) 222, 278, 427, 657
BmgT120I GGNCC 5 cut(s) 222, 278, 314, 427, 657
BmiI GGNNCC 6 cut(s) 223, 224, 315, 429, 503, 552
BmrFI CCNGG 2 cut(s) 170, 617
BmsI GCATC 2 cut(s) 238, 708
BpiI GAAGAC 2 cut(s) 337, 371
BplI GAGNNNNNCTC 2 cut(s) 590, 622
BpmI CTGGAG 1 cut(s) 617
Bpu10I CCTNAGC 1 cut(s) 476
BpuMI CCSGG 1 cut(s) 170
BsaAI YACGTR 1 cut(s) 384
BsaHI GRCGYC 4 cut(s) 266, 417, 459, 502
BsaJI CCNNGG 5 cut(s) 168, 324, 505, 546, 755
BsaXI ACNNNNNCTCC 2 cut(s) 101, 131
Bsc4I CCNNNNNNNGG 2 cut(s) 232, 406
Bse118I RCCGGY 1 cut(s) 650
Bse1I ACTGG 1 cut(s) 731
Bse3DI GCAATG 1 cut(s) 445
BseBI CCWGG 1 cut(s) 617
BseDI CCNNGG 5 cut(s) 168, 324, 505, 546, 755
BseGI GGATG 1 cut(s) 723
BseLI CCNNNNNNNGG 2 cut(s) 232, 406
BseMI GCAATG 1 cut(s) 445
BseMII CTCAG 4 cut(s) 27, 206, 208, 591
BseNI ACTGG 1 cut(s) 731
BseRI GAGGAG 2 cut(s) 136, 154
BseX3I CGGCCG 1 cut(s) 337
BseXI GCAGC 5 cut(s) 174, 274, 696, 717, 724
BseYI CCCAGC 1 cut(s) 225
Bsh1236I CGCG 3 cut(s) 342, 519, 635
Bsh1285I CGRYCG 1 cut(s) 340
BshFI GGCC 4 cut(s) 316, 329, 339, 650
BshNI GGYRCC 1 cut(s) 501
BsiEI CGRYCG 1 cut(s) 340
BsiHKAI GWGCWC 4 cut(s) 242, 248, 383, 539
BsiSI CCGG 2 cut(s) 170, 651
BslFI GGGAC 1 cut(s) 208
BslI CCNNNNNNNGG 2 cut(s) 232, 406
BsmAI GTCTC 2 cut(s) 104, 195
BsmBI CGTCTC 1 cut(s) 195
BsmFI GGGAC 1 cut(s) 208
BsnI GGCC 4 cut(s) 316, 329, 339, 650
Bsp1286I GDGCHC 4 cut(s) 242, 248, 383, 539
Bsp143I GATC 1 cut(s) 297
Bsp19I CCATGG 2 cut(s) 546, 755
BspANI GGCC 4 cut(s) 316, 329, 339, 650
BspCNI CTCAG 4 cut(s) 26, 207, 207, 590
BspFNI CGCG 3 cut(s) 342, 519, 635
BspLI GGNNCC 6 cut(s) 223, 224, 315, 429, 503, 552
BspMAI CTGCAG 1 cut(s) 714
BspPI GGATC 1 cut(s) 305
BspQI GCTCTTC 1 cut(s) 371
BspT107I GGYRCC 1 cut(s) 501
BsrBI CCGCTC 1 cut(s) 124
BsrDI GCAATG 1 cut(s) 445
BsrFI RCCGGY 1 cut(s) 650
BsrI ACTGG 1 cut(s) 731
BssAI RCCGGY 1 cut(s) 650
BssECI CCNNGG 5 cut(s) 168, 324, 505, 546, 755
BssMI GATC 1 cut(s) 297
BssNI GRCGYC 4 cut(s) 266, 417, 459, 502
BssSI CACGAG 1 cut(s) 241
BssT1I CCWWGG 3 cut(s) 505, 546, 755
Bst2BI CACGAG 1 cut(s) 241
Bst2UI CCWGG 1 cut(s) 617
Bst4CI ACNGT 2 cut(s) 45, 306
Bst6I CTCTTC 3 cut(s) 365, 371, 635
BstACI GRCGYC 4 cut(s) 266, 417, 459, 502
BstAPI GCANNNNNTGC 2 cut(s) 718, 730
BstBAI YACGTR 1 cut(s) 384
BstC8I GCNNGC 2 cut(s) 608, 668
BstDEI CTNAG 5 cut(s) 13, 194, 215, 476, 577
BstDSI CCRYGG 3 cut(s) 324, 546, 755
BstF5I GGATG 1 cut(s) 723
BstFNI CGCG 3 cut(s) 342, 519, 635
BstH2I RGCGCY 1 cut(s) 505
BstHHI GCGC 2 cut(s) 504, 637
BstKTI GATC 1 cut(s) 300
BstMAI GTCTC 2 cut(s) 104, 195
BstMBI GATC 1 cut(s) 297
BstMCI CGRYCG 1 cut(s) 340
BstMWI GCNNNNNNNGC 5 cut(s) 607, 718, 721, 727, 730
BstNI CCWGG 1 cut(s) 617
BstSCI CCNGG 2 cut(s) 168, 615
BstSFI CTRYAG 1 cut(s) 710
BstUI CGCG 3 cut(s) 342, 519, 635
BstV1I GCAGC 5 cut(s) 174, 274, 696, 717, 724
BstV2I GAAGAC 2 cut(s) 337, 371
BstXI CCANNNNNNTGG 3 cut(s) 403, 547, 705
BstZI CGGCCG 1 cut(s) 337
BsuRI GGCC 4 cut(s) 316, 329, 339, 650
BtgI CCRYGG 3 cut(s) 324, 546, 755
BtsCI GGATG 1 cut(s) 723
BtsIMutI CAGTG 2 cut(s) 41, 724
Cac8I GCNNGC 2 cut(s) 608, 668
CaiI CAGNNNCTG 1 cut(s) 187
CfoI GCGC 2 cut(s) 504, 637
Cfr10I RCCGGY 1 cut(s) 650
Cfr13I GGNCC 5 cut(s) 222, 278, 314, 427, 657
CseI GACGC 4 cut(s) 193, 331, 406, 448
Csp6I GTAC 4 cut(s) 212, 412, 454, 490
CviAII CATG 4 cut(s) 401, 495, 547, 756
CviQI GTAC 4 cut(s) 212, 412, 454, 490
DdeI CTNAG 5 cut(s) 13, 194, 215, 476, 577
DinI GGCGCC 1 cut(s) 503
DpnI GATC 1 cut(s) 299
DpnII GATC 1 cut(s) 297
EaeI YGGCCR 3 cut(s) 327, 337, 648
EagI CGGCCG 1 cut(s) 337
Eam1104I CTCTTC 3 cut(s) 365, 371, 635
EarI CTCTTC 3 cut(s) 365, 371, 635
EciI GGCGGA 1 cut(s) 310
Ecl136II GAGCTC 1 cut(s) 537
EclXI CGGCCG 1 cut(s) 337
Eco130I CCWWGG 3 cut(s) 505, 546, 755
Eco24I GRGCYC 1 cut(s) 539
Eco47I GGWCC 4 cut(s) 222, 278, 427, 657
Eco52I CGGCCG 1 cut(s) 337
Eco53kI GAGCTC 1 cut(s) 537
Eco72I CACGTG 1 cut(s) 384
EcoICRI GAGCTC 1 cut(s) 537
EcoO109I RGGNCCY 1 cut(s) 222
EcoRII CCWGG 1 cut(s) 615
EcoT14I CCWWGG 3 cut(s) 505, 546, 755
EcoT38I GRGCYC 1 cut(s) 539
EgeI GGCGCC 1 cut(s) 503
EheI GGCGCC 1 cut(s) 503
ErhI CCWWGG 3 cut(s) 505, 546, 755
Esp3I CGTCTC 1 cut(s) 195
FaeI CATG 4 cut(s) 404, 498, 550, 759
FaiI YATR 6 cut(s) 302, 402, 496, 548, 757, 769
FaqI GGGAC 1 cut(s) 208
FatI CATG 4 cut(s) 400, 494, 546, 755
FauI CCCGC 3 cut(s) 129, 292, 512
FblI GTMKAC 1 cut(s) 447
Fnu4HI GCNGC 8 cut(s) 188, 255, 288, 340, 710, 713, 722, 731
FokI GGATG 1 cut(s) 730
FriOI GRGCYC 1 cut(s) 539
Fsp4HI GCNGC 8 cut(s) 188, 255, 288, 340, 710, 713, 722, 731
GlaI GCGC 2 cut(s) 503, 636
GluI GCNGC 8 cut(s) 188, 255, 288, 340, 710, 713, 722, 731
GsaI CCCAGC 1 cut(s) 229
GsuI CTGGAG 1 cut(s) 617
HaeII RGCGCY 1 cut(s) 505
HaeIII GGCC 4 cut(s) 316, 329, 339, 650
HapII CCGG 2 cut(s) 170, 651
HgaI GACGC 4 cut(s) 193, 331, 406, 448
HhaI GCGC 2 cut(s) 504, 637
Hin1I GRCGYC 4 cut(s) 266, 417, 459, 502
Hin1II CATG 4 cut(s) 404, 498, 550, 759
Hin6I GCGC 2 cut(s) 502, 635
HinP1I GCGC 2 cut(s) 502, 635
HincII GTYRAC 2 cut(s) 448, 681
HindII GTYRAC 2 cut(s) 448, 681
HpaII CCGG 2 cut(s) 170, 651
HphI GGTGA 1 cut(s) 148
Hpy166II GTNNAC 4 cut(s) 281, 448, 626, 681
Hpy188I TCNGA 3 cut(s) 354, 427, 580
Hpy188III TCNNGA 2 cut(s) 233, 262
Hpy8I GTNNAC 4 cut(s) 281, 448, 626, 681
Hpy99I CGWCG 4 cut(s) 422, 449, 452, 464
HpyAV CCTTC 1 cut(s) 83
HpyCH4III ACNGT 2 cut(s) 45, 306
HpyCH4IV ACGT 2 cut(s) 266, 383
HpyCH4V TGCA 1 cut(s) 712
HpyF10VI GCNNNNNNNGC 5 cut(s) 607, 718, 721, 727, 730
HpyF3I CTNAG 5 cut(s) 13, 194, 215, 476, 577
HpySE526I ACGT 2 cut(s) 266, 383
Hsp92I GRCGYC 4 cut(s) 266, 417, 459, 502
Hsp92II CATG 4 cut(s) 404, 498, 550, 759
HspAI GCGC 2 cut(s) 502, 635
KasI GGCGCC 1 cut(s) 501
KflI GGGWCCC 1 cut(s) 222
Kzo9I GATC 1 cut(s) 297
LguI GCTCTTC 1 cut(s) 371
LmnI GCTCC 4 cut(s) 122, 534, 542, 598
Lsp1109I GCAGC 5 cut(s) 174, 274, 696, 717, 724
LweI GCATC 2 cut(s) 238, 708
MaeII ACGT 2 cut(s) 266, 383
MaeIII GTNAC 2 cut(s) 262, 267
MalI GATC 1 cut(s) 299
MbiI CCGCTC 1 cut(s) 124
MboI GATC 1 cut(s) 297
MboII GAAGA 7 cut(s) 337, 373, 376, 382, 388, 535, 622
MfeI CAATTG 1 cut(s) 249
MhlI GDGCHC 4 cut(s) 242, 248, 383, 539
MlsI TGGCCA 1 cut(s) 329
MluCI AATT 1 cut(s) 249
MluNI TGGCCA 1 cut(s) 329
Mly113I GGCGCC 1 cut(s) 502
MmeI TCCRAC 1 cut(s) 635
MnlI CCTC 7 cut(s) 95, 123, 157, 175, 269, 366, 574
Mox20I TGGCCA 1 cut(s) 329
MscI TGGCCA 1 cut(s) 329
MseI TTAA 1 cut(s) 761
Msp20I TGGCCA 1 cut(s) 329
MspA1I CMGCKG 2 cut(s) 287, 715
MspI CCGG 2 cut(s) 170, 651
MspR9I CCNGG 2 cut(s) 170, 617
MunI CAATTG 1 cut(s) 249
MvaI CCWGG 1 cut(s) 617
MvnI CGCG 3 cut(s) 342, 519, 635
MwoI GCNNNNNNNGC 5 cut(s) 607, 718, 721, 727, 730
NarI GGCGCC 1 cut(s) 502
NciI CCSGG 1 cut(s) 170
NcoI CCATGG 2 cut(s) 546, 755
NdeII GATC 1 cut(s) 297
NlaIII CATG 4 cut(s) 404, 498, 550, 759
NlaIV GGNNCC 6 cut(s) 223, 224, 315, 429, 503, 552
NmeAIII GCCGAG 1 cut(s) 224
NmuCI GTSAC 2 cut(s) 262, 267
PciSI GCTCTTC 1 cut(s) 371
PcsI WCGNNNNNNNCGW 2 cut(s) 441, 456
PflMI CCANNNNNTGG 1 cut(s) 406
PkrI GCNGC 8 cut(s) 189, 256, 289, 341, 711, 714, 723, 732
PluTI GGCGCC 1 cut(s) 505
PmaCI CACGTG 1 cut(s) 384
PmlI CACGTG 1 cut(s) 384
Ppu21I YACGTR 1 cut(s) 384
PpuMI RGGWCCY 1 cut(s) 222
Psp124BI GAGCTC 1 cut(s) 539
Psp5II RGGWCCY 1 cut(s) 222
Psp6I CCWGG 1 cut(s) 615
PspCI CACGTG 1 cut(s) 384
PspFI CCCAGC 1 cut(s) 225
PspGI CCWGG 1 cut(s) 615
PspN4I GGNNCC 6 cut(s) 223, 224, 315, 429, 503, 552
PspPI GGNCC 5 cut(s) 222, 278, 314, 427, 657
PspPPI RGGWCCY 1 cut(s) 222
PstI CTGCAG 1 cut(s) 714
PstNI CAGNNNCTG 1 cut(s) 187
RsaI GTAC 4 cut(s) 213, 413, 455, 491
RsaNI GTAC 4 cut(s) 212, 412, 454, 490
SacI GAGCTC 1 cut(s) 539
SalI GTCGAC 1 cut(s) 446
SapI GCTCTTC 1 cut(s) 371
SaqAI TTAA 1 cut(s) 761
SatI GCNGC 8 cut(s) 188, 255, 288, 340, 710, 713, 722, 731
Sau3AI GATC 1 cut(s) 297
Sau96I GGNCC 5 cut(s) 222, 278, 314, 427, 657
ScaI AGTACT 1 cut(s) 213
ScrFI CCNGG 2 cut(s) 170, 617
SduI GDGCHC 4 cut(s) 242, 248, 383, 539
SfaNI GCATC 2 cut(s) 238, 708
SfcI CTRYAG 1 cut(s) 710
SfoI GGCGCC 1 cut(s) 503
SgrDI CGTCGACG 1 cut(s) 446
SinI GGWCC 4 cut(s) 222, 278, 427, 657
Sse9I AATT 1 cut(s) 249
SspDI GGCGCC 1 cut(s) 501
SstI GAGCTC 1 cut(s) 539
StyD4I CCNGG 2 cut(s) 168, 615
StyI CCWWGG 3 cut(s) 505, 546, 755
TaaI ACNGT 2 cut(s) 45, 306
TaiI ACGT 2 cut(s) 269, 386
TaqI TCGA 1 cut(s) 447
TasI AATT 1 cut(s) 249
TatI WGTACW 1 cut(s) 211
TauI GCSGC 3 cut(s) 257, 342, 724
Tru1I TTAA 1 cut(s) 761
Tru9I TTAA 1 cut(s) 761
TscAI CASTG 2 cut(s) 48, 731
TseFI GTSAC 2 cut(s) 262, 267
TseI GCWGC 5 cut(s) 187, 287, 709, 712, 730
Tsp45I GTSAC 2 cut(s) 262, 267
TspGWI ACGGA 1 cut(s) 338
TspRI CASTG 2 cut(s) 48, 731
Van91I CCANNNNNTGG 1 cut(s) 406
VpaK11BI GGWCC 4 cut(s) 222, 278, 427, 657
XcmI CCANNNNNNNNNTGG 2 cut(s) 400, 403
XmiI GTMKAC 1 cut(s) 447
ZraI GACGTC 1 cut(s) 267
ZrmI AGTACT 1 cut(s) 213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.