Rorug07G0331400
ERF Family

Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin- like GTPase superfamily. TrmE GTPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0012258
Physical Location & Seq
Reverse (-)
36295080 .. 36318484
23405 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0331400.1

Sequence Viewer

Length: 4878 bp
ATGGGGAGCCGTGCGTTTTGTGGTATTATCATCTTGATTTGTTTGATTGGCTCGGTTTCGGTTTCGGTTTCAGCTCTGAATCGAAGGCCCAAGAATGTGCAGGTCGCCGTTCGGGCCAAGTGGTCCCCCACTCCATTGCTCCTAGAAGCTGGTGAATTGCTATCTAGAGAACAGAAAGATCATTTTTGGGAATTCATTGACATCTGGCATCATACTGATAAGGATGATCTTGATTCTTATAATGCTAAGGGTTGCCTAAAAAAAATTTTGAAACATGGGCTCTCTATTTTGAGTGAACCATTGGCTTCTTTATTTGAATTCTCTCTTACTTTGAGATCTGCATCCCCAAGATTGGTGCTTTACCGGCAATTAGCTGAAGAGTCTCTTTCATCTTTCCCACCAGTTGATGAAACTAATTCAAGAAATGCCAGCGGAAATTCAGAAACAAATGACCACGTGGAAAGTCAAAAGTCAGATCCTTTGAATGTTGGTCTAAACCCAAGAAGTCCTAATGGAAAATGTTGTTGGGTGGATACTGGAGATGCTCTGTTCTTTGATGCTGTAGACTTAAAAAGTTGGCTTAACAGTCCTAGAGATTCTTCTAGGGATTCATTTCAGCAGCCTGAACTCTTCGAATTTGACCACATCCACTTTGATTCAATTGTTGGAAGTCCAGTTGCTGTTCTTTATGGAGCTCTTGGAACTGAGTGCTTTAGGGAGTTTCATGTCACCCTAGTTGAAGCTGCAAAAGAGGGTAATGTTAAGTATGTGGTTAGGCAAGTATTACCTTCTGGCTGCGAAGCAGAGATTGACCGTTGTGGTGCTGTTGGTACAAAAGATTCGTTAAACTTGGGTGGTTATGGAGTCGAACTTGCTTTGAAGAACATGGAATATAAGGCCATGGACGATAGTGCAATAAAGAAAGGTGTCACGTTAGAAGATCCTCGGACTGAAGATCTCAGCCAAGAAGTCAGAGGGTTTATATTCTCTAAAATTCTGGAGCGTAAACCCGAACTCACATCAGAGATAATGGCTTTTAGGGATTATCTTTTGTCATCAACAGTATCTGATACACTTGATGTTTGGGAACTAAAAGATTTAGGACATCAAACTGCACAGAGGATAGTGCATGCCACTGATCCTCTGCAGTCAATGCAAGAAATTAATCAAAACTTTCCCAGTGTTGTCTCTTCCTTATCTCGCATGAAGCTCAATGATTCAGTTAAAGATGAAATTACTGCAAACCAGAGAATGATTCAACCTGGAAAGTCCTTGATGGCTCTGAATGGTGCTTTAGTCAATATTGAAGATATTGACCTGTATCTGTTGGTTGACTTGGTCCATCAGGATTTATTATTGGCTGATCACTTTTCAAAGTTGAAGATACCTCATAGCACTGTACGGAAACTCCTGTCAACTCTGCCTCCTCCAGAATCTAATATGTTGCGTGTAGATTTTCGCTCGAATCATGTTCATTATTTAAATAACTTGGAAGAAGATGCTATGTATAAGCGTTGGAGGAGCAATCTTAATGAGATTTTGATGCCAGTGTTCCCTGGACAGCTACGTTATATACGCAAAAACCTTTTCCATGCTGTTTCCGTTATTGATCCATCAACTGTTTGTGGTCTTGAGTCTATTGATGTGCTTATTTCTTTGTATGAGAATAACTTTCCCATGAGATTTGGGGTTGTACTGTATTCTTCGAAGTTGATCAAGCAAATTGAAACTAGCAGTGATGATAGTAAAATTGAGGAGGATATATCCAACCTGATAATACGCCTCTTTATTTATATCAAGGAAAATCATGGAATTCAAACAGCTTTCCAATTTTTGAGCAATATAAATAAATTGCGGGTTGAGTCTGATGGTTCGGCTGATGATCTTGAAATGCACTATGTAGAAGGAGCATTTGTAGAAACAGTATTACCAAAGGTAAAATCTCCGCCTCAAGGCATACTGCTGAAGCTGCAGAAGGAGCAAACTTACAAGGAACTTGCACATGAAAGCACCATGTTTGTCTTTAAGCTGGGCTTGGCTAAGCTGCAGTGCAGTTTGCTGATGAATGGACTTGTGTTGGATTCTAGTGAGGAGGCTCTTATAAATTCCATGAACGATGAGATGCCCAGAATACAGGAGCAAGTATACTATGGCCATATAAATTCTCAAACAAATGTTCTTGACAAGTTTTTGTCAGAAAGTGGTACCACTCGCTATAATCCACAGATTATTGCTGGTGGAAAACCAAGGTTCACATCTCTATGCACTTCTGTTCTTGGAGGGGAAGGTGTCTTCAATGATATTGGTTATTTGCATTCCCCCGAAACTGTGGATGATTTGAAGCCTGTGACTCATCTTCTAGTGGTTGATGTTGCATCAAAGAAAGGGATGAAGCTGCTTCATGAAGCCTTACACTATCTGATTGAAGGGTCTAATAGGGCTCGAGTAGGAGTGCTTTTCGGTGTCAATCAGGGTGCTGATCTTTCTAGTCTCCTTTTTGTTGAAGTATTTCAAATCACTGCATCATTGCACAGCCACAAGAAGGATGTGTTACTTTTCTTGGATCAGGTGTGCTCCTTGTTTGAACATAATCACATGCTTGCACCGTCTGAAGGTGCGGAGAGCACTCGAGCATTCATAGATAAAGTTTCTGAATTGGCTGAGACAAATGGGTTATCATCAAAGGCCTATAAATCAGCTCTTTCGGACTTTTCTGCTGACAAATTGAGAAAACAGTTGAATAAGGTGGCACAGTTTTTATATAGGCAACTTGGCCTTGATTCTGGTGTTAATGCAGTCATTACTAACGGACGGGTAACGGTTGTAAATGAGGAGAGCACATTGTTGAGCCATGATTTAAATCTTCTTGAGTCTCTGGAGTTTACACAGAGAATAAAGCATATTGTTGAAATAATAGAAGAAGTGAAGTGGCAGGATGTGGATCCAGACACACTGACAAGCAAATTCATTAGTGATACTATCATGTTTGTGTCTTCTTCAATGGCTATGCGGGATCGAAGTTCTGAAGGTGCACGCTTTGAGGTTTTGAATGCAGAATATAGTGCCATCGTTCTGAACAATGAAAATTCTAGTATCCATATAGATGCAGTTATTGATCCTTTAAGTCCATCTGGCCAGAAGCTATCATCAATTCTTCGGGTTCTATGGAAATATGTTCAGCCTAGCATGAGAATTGTACTAAATCCGTTGAGTTCTCTTGTTGATCTTCCTCTGAAGAATTACTACAGATATGTAGTACCGACAGTGGATGACTTCAGCAGCACTGACTACACAGTAAATGGGCCAAAAGCCTTTTTTGCAAACATGCCACTGTCCAAAACCCTCACTATGAATCTTGATGTTCCTGATCCATGGCTTGTTGAGCCTGTTATTGCAGTGCATGACTTGGATAATATTTTGCTTGAGAATCTTGGTGAGACAAGAACATTACAAGCTGTTTTTGAACTTGAAGCTCTTGTTTTGACGGGTCATTGTTCTGAGAAAGATCATGATCCGCCCAGAGGCCTTCAGTTAATTATTGGTACCAAGAGTGCACCCCATTTGGTTGATACTCTTGTGATGGCCAATCTGGGTTATTGGCAAATGAAAGTCTCTCCTGGTGTTTGGTACCTACAACTTGCTCCAGGAAGAAGTTCTGAGCTTTATGTTTTAAAAGAGGATGGTGATGGAAGTCAGAGCAAAACATTATCAAAACGCATCACCATTAATGATCTGCGGGGTAGAGTAGTTCACCTGGAAGTAGTAAAGAAAAAGGGAAAGGAGCATGAAAAATTGCTGCTTTCTGATGTTAATGACAAGACACAGGATGCTACCGAGGGAAGTAGCTGGAACTCCAACTTTATAAAATGGGCTTCTGGTTTAATTGGTGGCAGTGAGCACTCAAAACAGAGTGAAAGTACTTCATCGGAGAAGGGAAAAGGTGGACGCCATGGAAAAACAATAAATATTTTTTCTATTGCTTCTGGACACTTATACGAACGGTTTTTGAAAATCATGATTTTAAGTGTCTTGAAGAACACCCGTCGTCCAGTTAAATTCTGGTTCATAAAGAACTATCTCTCTCCTCAATTCAAGGATGTGATTCCACATATGGCTCATGAATATGGCTTTGAGTATCAGCTAATCACCTACAAGTGGCCTACATGGTTGCACAAGCAAAAAGAAAAGCAACGAATTATTTGGGCCTATAAAATTTTGTTCCTTGATGTTATATTCCCCCTTTCGTTAGAAAAGGTCATCTTTGTTGATGCAGATCAGATTGTCAGGGCAGACATGGGAGAACTCTATGACATGGATATAAAAGGAAGACCTCTGGCATATACTCCTTTCTGTGACAACAACAAGGATATGGATGGATATCGGTTTTGGAGACAAGGTTTCTGGAAAGAACATTTACGGGGGAGATCTTATCATATAAGTGCTTTGTATGTCGTTGATTTGAAGAAATTTCGAGAGACTGCAGCAGGAGATAATCTGAGAGTTTTCTATGAGACTCTTAGCAAGGACCCAAATAGTCTATCAAATTTGGATCAGGATCTTCCAAACTATGCTCAACATACAGTGCCCATATTTTCATTACCACAAGAATGGCTGTGGTGTGAGTCATGGTGTGGTAATGCCACAAAAGCCAAAGCAAAAACCATAGATCTTTGCAACAACCCAATGACAAAGGAACCCAAGCTCCAGGGTGCCAGAAGAATAGTTTCTGAGTGGCCAGATCTTGATTTGGAGGCTAGGCAGTTCACTGCAAAAATCTTAGGTGATGAAGTCGCAATCCAAGAACCTCCACCAGATCCTAATCAATCAGGATCAGTGATGGACAGCCCACCAGACCTGGAATCTAAGGCAGAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MMR_HSR1 MnmE_helical TrmE_N

Protein Analysis

1625

Amino Acids

183.13

Weight (kDa)

5.68

Isoelectric Point (pI)

38.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin_12 PF18400 44 - 268 1.2e-48 Thioredoxin-like domain
Thioredoxin_13 PF18401 357 - 472 5e-33 Thioredoxin-like domain
Thioredoxin_14 PF18402 485 - 743 2.6e-57 Thioredoxin-like domain
Thioredoxin_15 PF18403 763 - 1002 6.8e-41 Thioredoxin-like domain
UDP-g_GGTase PF06427 1163 - 1266 4.2e-29 UDP-glucose:Glycoprotein Glucosyltransferase
Glyco_transf_24 PF18404 1321 - 1587 6.9e-144 Glucosyltransferase 24
Glyco_transf_8 PF01501 1332 - 1544 1.4e-07 Glycosyl transferase family 8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012258)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78010
fragaria_vesca FvH4_5g38010 FvH4_5g38010
malus_domestica MD08G1177300.v1.1 MD15G1355000.v1.1
prunus_persica Prupe.1G511900_v2.0.a1
pyrus_communis pycom08g15220
rosa_chinensis RchiOBHm_Chr7g0239951
rosa_laevigata RLG00000000715
rosa_multiflora Rmu_sc0000540.1_g000022 Rmu_sc0006825.1_g000001 Rmu_sc0035965.1_g000001
rosa_roxburghii Rroxscaffold_3G00220600
rosa_rugosa Rorug07G0331400
rosa_samantha Rh7AG485100 Rh7BG458100 Rh7CG502400 Rh7DG470700
rosa_wichuraiana Rw7G040690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 240, 2102, 3863
Acc36I ACCTGC 1 cut(s) 91
Acc65I GGTACC 3 cut(s) 2204, 3542, 3627
AccB1I GGYRCC 4 cut(s) 2204, 3542, 3627, 4709
AccI GTMKAC 2 cut(s) 564, 2145
AciI CCGC 7 cut(s) 432, 1855, 1946, 2618, 3010, 3515, 3736
AcoI YGGCCR 4 cut(s) 2152, 3133, 3582, 4733
AcuI CTGAAG 8 cut(s) 396, 972, 1985, 2631, 3045, 3254, 3259, 3512
AcvI CACGTG 1 cut(s) 457
AcyI GRCGYC 1 cut(s) 3946
AfaI GTAC 9 cut(s) 832, 1401, 1695, 2206, 3198, 3258, 3544, 3629, 3919
AfiI CCNNNNNNNGG 6 cut(s) 352, 1952, 2542, 2612, 3521, 4155
AjnI CCWGG 7 cut(s) 1261, 1555, 3616, 3643, 3753, 4704, 4854
AjuI GAANNNNNNNTTGG 2 cut(s) 508, 540
AloI GAACNNNNNNTCC 2 cut(s) 4686, 4718
Alw21I GWGCWC 7 cut(s) 697, 2576, 2627, 2840, 3034, 3556, 3900
Alw44I GTGCAC 2 cut(s) 3030, 3552
AlwNI CAGNNNCTG 2 cut(s) 680, 1067
Ama87I CYCGRG 2 cut(s) 2442, 2628
ApaLI GTGCAC 2 cut(s) 3030, 3552
ApeKI GCWGC 9 cut(s) 619, 743, 795, 1969, 2044, 2395, 3279, 3796, 4481
AseI ATTAAT 2 cut(s) 1164, 3726
Asp700I GAANNNNTTC 3 cut(s) 2508, 3652, 4723
Asp718I GGTACC 3 cut(s) 2204, 3542, 3627
AspS9I GGNCC 7 cut(s) 87, 114, 123, 1339, 3302, 4203, 4525
AsuHPI GGTGA 8 cut(s) 164, 721, 3446, 3695, 3712, 3743, 4138, 4793
AsuII TTCGAA 2 cut(s) 633, 1706
AvaI CYCGRG 2 cut(s) 2442, 2628
AvaII GGWCC 3 cut(s) 123, 1339, 4525
BaeGI GKGCMC 3 cut(s) 3034, 3556, 4587
BaeI ACNNNNGTAYC 2 cut(s) 3561, 3594
BalI TGGCCA 4 cut(s) 2154, 3135, 3584, 4735
BamHI GGATCC 1 cut(s) 2941
BanI GGYRCC 4 cut(s) 2204, 3542, 3627, 4709
BanII GRGCYC 3 cut(s) 282, 697, 2443
BarI GAAGNNNNNNTAC 2 cut(s) 3227, 3259
BbrPI CACGTG 1 cut(s) 457
BbsI GAAGAC 3 cut(s) 2284, 2985, 4333
Bbv12I GWGCWC 7 cut(s) 697, 2576, 2627, 2840, 3034, 3556, 3900
BbvI GCAGC 9 cut(s) 631, 730, 782, 1956, 2031, 2382, 3291, 3783, 4493
BceAI ACGGC 1 cut(s) 92
BciT130I CCWGG 7 cut(s) 1263, 1557, 3618, 3645, 3755, 4706, 4856
BciVI GTATCC 2 cut(s) 526, 3104
BclI TGATCA 2 cut(s) 1363, 1713
BfmI CTRYAG 6 cut(s) 561, 1145, 1970, 2045, 3244, 4479
BfuAI ACCTGC 1 cut(s) 91
BfuI GTATCC 2 cut(s) 526, 3104
BglI GCCNNNNNGGC 1 cut(s) 113
BglII AGATCT 5 cut(s) 335, 955, 4424, 4666, 4738
BisI GCNGC 9 cut(s) 620, 744, 796, 1970, 2045, 2396, 3280, 3797, 4482
BlpI GCTNAGC 1 cut(s) 2040
BlsI GCNGC 9 cut(s) 621, 745, 797, 1971, 2046, 2397, 3281, 3798, 4483
BmcAI AGTACT 1 cut(s) 3919
Bme1390I CCNGG 7 cut(s) 1263, 1557, 3618, 3645, 3755, 4706, 4856
Bme18I GGWCC 3 cut(s) 123, 1339, 4525
BmeT110I CYCGRG 2 cut(s) 2442, 2628
BmgT120I GGNCC 7 cut(s) 87, 114, 123, 1339, 3302, 4203, 4525
BmiI GGNNCC 9 cut(s) 8, 125, 2206, 2943, 3544, 3629, 4527, 4695, 4711
BmrFI CCNGG 7 cut(s) 1263, 1557, 3618, 3645, 3755, 4706, 4856
BmrI ACTGGG 1 cut(s) 1173
BmuI ACTGGG 1 cut(s) 1173
BpiI GAAGAC 3 cut(s) 2284, 2985, 4333
BpmI CTGGAG 6 cut(s) 558, 1019, 1413, 2897, 3627, 4688
Bpu10I CCTNAGC 1 cut(s) 246
Bpu1102I GCTNAGC 1 cut(s) 2040
Bpu14I TTCGAA 2 cut(s) 633, 1706
BpuEI CTTGAG 4 cut(s) 1652, 1935, 2888, 3443
BsaAI YACGTR 1 cut(s) 457
BsaBI GATNNNNATC 8 cut(s) 340, 2859, 2940, 3510, 4272, 4377, 4554, 4818
BsaHI GRCGYC 1 cut(s) 3946
BsaJI CCNNGG 8 cut(s) 900, 944, 1555, 2246, 3371, 3834, 3949, 4705
Bsc4I CCNNNNNNNGG 6 cut(s) 352, 1952, 2542, 2612, 3521, 4155
Bse118I RCCGGY 1 cut(s) 363
Bse1I ACTGG 6 cut(s) 401, 541, 674, 1179, 1547, 4049
Bse3DI GCAATG 2 cut(s) 134, 2525
Bse8I GATNNNNATC 8 cut(s) 340, 2859, 2940, 3510, 4272, 4377, 4554, 4818
BseBI CCWGG 7 cut(s) 1263, 1557, 3618, 3645, 3755, 4706, 4856
BseDI CCNNGG 8 cut(s) 900, 944, 1555, 2246, 3371, 3834, 3949, 4705
BseJI GATNNNNATC 8 cut(s) 340, 2859, 2940, 3510, 4272, 4377, 4554, 4818
BseLI CCNNNNNNNGG 6 cut(s) 352, 1952, 2542, 2612, 3521, 4155
BseMI GCAATG 2 cut(s) 134, 2525
BseMII CTCAG 7 cut(s) 696, 973, 2652, 3489, 3648, 4487, 4719
BseNI ACTGG 6 cut(s) 401, 541, 674, 1179, 1547, 4049
BseRI GAGGAG 6 cut(s) 1416, 1534, 1769, 2105, 2846, 4074
BseSI GKGCMC 3 cut(s) 3034, 3556, 4587
BseXI GCAGC 9 cut(s) 631, 730, 782, 1956, 2031, 2382, 3291, 3783, 4493
BseYI CCCAGC 1 cut(s) 2029
BsgI GTGCAG 3 cut(s) 119, 1098, 2071
BshNI GGYRCC 4 cut(s) 2204, 3542, 3627, 4709
BsiHKAI GWGCWC 7 cut(s) 697, 2576, 2627, 2840, 3034, 3556, 3900
BsiHKCI CYCGRG 2 cut(s) 2442, 2628
BsiSI CCGG 1 cut(s) 364
BslFI GGGAC 1 cut(s) 109
BslI CCNNNNNNNGG 6 cut(s) 352, 1952, 2542, 2612, 3521, 4155
BsmFI GGGAC 1 cut(s) 109
BsmI GAATGC 3 cut(s) 2314, 2633, 3055
BsoBI CYCGRG 2 cut(s) 2442, 2628
Bsp119I TTCGAA 2 cut(s) 633, 1706
Bsp1720I GCTNAGC 1 cut(s) 2040
Bsp19I CCATGG 3 cut(s) 900, 3371, 3949
BspACI CCGC 7 cut(s) 432, 1855, 1946, 2618, 3010, 3515, 3736
BspCNI CTCAG 7 cut(s) 697, 972, 2653, 3490, 3649, 4488, 4720
BspHI TCATGA 4 cut(s) 2401, 3508, 4014, 4117
BspLI GGNNCC 9 cut(s) 8, 125, 2206, 2943, 3544, 3629, 4527, 4695, 4711
BspMAI CTGCAG 4 cut(s) 1149, 1974, 2049, 4483
BspMI ACCTGC 1 cut(s) 91
BspT104I TTCGAA 2 cut(s) 633, 1706
BspT107I GGYRCC 4 cut(s) 2204, 3542, 3627, 4709
BsrDI GCAATG 2 cut(s) 134, 2525
BsrFI RCCGGY 1 cut(s) 363
BsrI ACTGG 6 cut(s) 401, 541, 674, 1179, 1547, 4049
BssAI RCCGGY 1 cut(s) 363
BssECI CCNNGG 8 cut(s) 900, 944, 1555, 2246, 3371, 3834, 3949, 4705
BssNAI GTATAC 1 cut(s) 2146
BssNI GRCGYC 1 cut(s) 3946
BssT1I CCWWGG 4 cut(s) 900, 2246, 3371, 3949
Bst1107I GTATAC 1 cut(s) 2146
Bst2UI CCWGG 7 cut(s) 1263, 1557, 3618, 3645, 3755, 4706, 4856
Bst6I CTCTTC 3 cut(s) 372, 635, 1195
BstACI GRCGYC 1 cut(s) 3946
BstAPI GCANNNNNTGC 1 cut(s) 1153
BstBAI YACGTR 1 cut(s) 457
BstBI TTCGAA 2 cut(s) 633, 1706
BstC8I GCNNGC 4 cut(s) 430, 1131, 2601, 3034
BstDSI CCRYGG 3 cut(s) 900, 3371, 3949
BstMWI GCNNNNNNNGC 8 cut(s) 113, 364, 1153, 1969, 1978, 3317, 3382, 4646
BstNI CCWGG 7 cut(s) 1263, 1557, 3618, 3645, 3755, 4706, 4856
BstNSI RCATGY 3 cut(s) 1133, 2599, 3328
BstSCI CCNGG 7 cut(s) 1261, 1555, 3616, 3643, 3753, 4704, 4854
BstSFI CTRYAG 6 cut(s) 561, 1145, 1970, 2045, 3244, 4479
BstSLI GKGCMC 3 cut(s) 3034, 3556, 4587
BstV1I GCAGC 9 cut(s) 631, 730, 782, 1956, 2031, 2382, 3291, 3783, 4493
BstV2I GAAGAC 3 cut(s) 2284, 2985, 4333
BstXI CCANNNNNNTGG 1 cut(s) 4608
BstZ17I GTATAC 1 cut(s) 2146
BsuI GTATCC 2 cut(s) 526, 3104
BtgI CCRYGG 3 cut(s) 900, 3371, 3949
BtsI GCAGTG 6 cut(s) 1741, 2054, 2517, 3402, 3898, 4764
BveI ACCTGC 1 cut(s) 91
Cac8I GCNNGC 4 cut(s) 430, 1131, 2601, 3034
CaiI CAGNNNCTG 2 cut(s) 680, 1067
CciI TCATGA 4 cut(s) 2401, 3508, 4014, 4117
Cfr10I RCCGGY 1 cut(s) 363
Cfr13I GGNCC 7 cut(s) 87, 114, 123, 1339, 3302, 4203, 4525
CseI GACGC 1 cut(s) 3954
Csp6I GTAC 9 cut(s) 831, 1400, 1694, 2205, 3197, 3257, 3543, 3628, 3918
CviQI GTAC 9 cut(s) 831, 1400, 1694, 2205, 3197, 3257, 3543, 3628, 3918
DraI TTTAAA 3 cut(s) 1482, 2859, 3672
EaeI YGGCCR 4 cut(s) 2152, 3133, 3582, 4733
Eam1104I CTCTTC 3 cut(s) 372, 635, 1195
EarI CTCTTC 3 cut(s) 372, 635, 1195
EciI GGCGGA 2 cut(s) 1935, 3504
Ecl136II GAGCTC 1 cut(s) 695
Eco130I CCWWGG 4 cut(s) 900, 2246, 3371, 3949
Eco147I AGGCCT 2 cut(s) 2687, 3525
Eco24I GRGCYC 3 cut(s) 282, 697, 2443
Eco32I GATATC 1 cut(s) 4379
Eco47I GGWCC 3 cut(s) 123, 1339, 4525
Eco53kI GAGCTC 1 cut(s) 695
Eco57I CTGAAG 8 cut(s) 396, 972, 1985, 2631, 3045, 3254, 3259, 3512
Eco72I CACGTG 1 cut(s) 457
Eco88I CYCGRG 2 cut(s) 2442, 2628
EcoICRI GAGCTC 1 cut(s) 695
EcoO109I RGGNCCY 1 cut(s) 4525
EcoRI GAATTC 3 cut(s) 191, 317, 1812
EcoRII CCWGG 7 cut(s) 1261, 1555, 3616, 3643, 3753, 4704, 4854
EcoRV GATATC 1 cut(s) 4379
EcoT14I CCWWGG 4 cut(s) 900, 2246, 3371, 3949
EcoT38I GRGCYC 3 cut(s) 282, 697, 2443
ErhI CCWWGG 4 cut(s) 900, 2246, 3371, 3949
FalI AAGNNNNNCTT 6 cut(s) 369, 401, 2018, 2050, 4244, 4276
FaqI GGGAC 1 cut(s) 109
FauI CCCGC 3 cut(s) 1848, 3003, 3729
FauNDI CATATG 1 cut(s) 4110
FbaI TGATCA 2 cut(s) 1363, 1713
FblI GTMKAC 2 cut(s) 564, 2145
Fnu4HI GCNGC 9 cut(s) 620, 744, 796, 1970, 2045, 2396, 3280, 3797, 4482
FriOI GRGCYC 3 cut(s) 282, 697, 2443
Fsp4HI GCNGC 9 cut(s) 620, 744, 796, 1970, 2045, 2396, 3280, 3797, 4482
GluI GCNGC 9 cut(s) 620, 744, 796, 1970, 2045, 2396, 3280, 3797, 4482
GsaI CCCAGC 1 cut(s) 2033
GsuI CTGGAG 6 cut(s) 558, 1019, 1413, 2897, 3627, 4688
HapII CCGG 1 cut(s) 364
HgaI GACGC 1 cut(s) 3954
Hin1I GRCGYC 1 cut(s) 3946
HincII GTYRAC 2 cut(s) 1333, 1416
HindII GTYRAC 2 cut(s) 1333, 1416
HpaII CCGG 1 cut(s) 364
HphI GGTGA 8 cut(s) 164, 721, 3446, 3695, 3712, 3743, 4138, 4793
Hpy99I CGWCG 1 cut(s) 4047
HpyCH4IV ACGT 3 cut(s) 456, 932, 1567
HpyF10VI GCNNNNNNNGC 8 cut(s) 113, 364, 1153, 1969, 1978, 3317, 3382, 4646
HpySE526I ACGT 3 cut(s) 456, 932, 1567
Hsp92I GRCGYC 1 cut(s) 3946
KpnI GGTACC 3 cut(s) 2208, 3546, 3631
Ksp22I TGATCA 2 cut(s) 1363, 1713
Lsp1109I GCAGC 9 cut(s) 631, 730, 782, 1956, 2031, 2382, 3291, 3783, 4493
MaeII ACGT 3 cut(s) 456, 932, 1567
MaeIII GTNAC 6 cut(s) 727, 928, 2347, 2550, 2815, 4352
MfeI CAATTG 1 cut(s) 660
MlsI TGGCCA 4 cut(s) 2154, 3135, 3584, 4735
MluNI TGGCCA 4 cut(s) 2154, 3135, 3584, 4735
MlyI GAGTC 8 cut(s) 389, 873, 1643, 1871, 2344, 2879, 4507, 4631
MmeI TCCRAC 5 cut(s) 646, 1496, 1791, 2058, 3879
Mox20I TGGCCA 4 cut(s) 2154, 3135, 3584, 4735
MroXI GAANNNNTTC 3 cut(s) 2508, 3652, 4723
MscI TGGCCA 4 cut(s) 2154, 3135, 3584, 4735
MslI CAYNNNNRTG 6 cut(s) 2260, 2987, 3102, 4122, 4437, 4606
Msp20I TGGCCA 4 cut(s) 2154, 3135, 3584, 4735
MspA1I CMGCKG 1 cut(s) 432
MspI CCGG 1 cut(s) 364
MspR9I CCNGG 7 cut(s) 1263, 1557, 3618, 3645, 3755, 4706, 4856
MunI CAATTG 1 cut(s) 660
Mva1269I GAATGC 3 cut(s) 2314, 2633, 3055
MvaI CCWGG 7 cut(s) 1263, 1557, 3618, 3645, 3755, 4706, 4856
MwoI GCNNNNNNNGC 8 cut(s) 113, 364, 1153, 1969, 1978, 3317, 3382, 4646
NcoI CCATGG 3 cut(s) 900, 3371, 3949
NdeI CATATG 1 cut(s) 4110
NlaIV GGNNCC 9 cut(s) 8, 125, 2206, 2943, 3544, 3629, 4527, 4695, 4711
NmuCI GTSAC 4 cut(s) 727, 928, 2347, 4352
NspI RCATGY 3 cut(s) 1133, 2599, 3328
NspV TTCGAA 2 cut(s) 633, 1706
PaeI GCATGC 1 cut(s) 1133
PaeR7I CTCGAG 2 cut(s) 2442, 2628
PagI TCATGA 4 cut(s) 2401, 3508, 4014, 4117
PceI AGGCCT 2 cut(s) 2687, 3525
PctI GAATGC 3 cut(s) 2314, 2633, 3055
PdmI GAANNNNTTC 3 cut(s) 2508, 3652, 4723
PflFI GACNNNGTC 1 cut(s) 1337
PfoI TCCNGGA 1 cut(s) 3643
PkrI GCNGC 9 cut(s) 621, 745, 797, 1971, 2046, 2397, 3281, 3798, 4483
PleI GAGTC 8 cut(s) 388, 872, 1642, 1870, 2344, 2878, 4507, 4630
PmaCI CACGTG 1 cut(s) 457
PmlI CACGTG 1 cut(s) 457
PpsI GAGTC 8 cut(s) 388, 872, 1642, 1870, 2344, 2878, 4507, 4630
Ppu21I YACGTR 1 cut(s) 457
PpuMI RGGWCCY 1 cut(s) 4525
PshBI ATTAAT 2 cut(s) 1164, 3726
PsiI TTATAA 3 cut(s) 240, 2102, 3863
Psp124BI GAGCTC 1 cut(s) 697
Psp5II RGGWCCY 1 cut(s) 4525
Psp6I CCWGG 7 cut(s) 1261, 1555, 3616, 3643, 3753, 4704, 4854
PspCI CACGTG 1 cut(s) 457
PspFI CCCAGC 1 cut(s) 2029
PspGI CCWGG 7 cut(s) 1261, 1555, 3616, 3643, 3753, 4704, 4854
PspN4I GGNNCC 9 cut(s) 8, 125, 2206, 2943, 3544, 3629, 4527, 4695, 4711
PspPI GGNCC 7 cut(s) 87, 114, 123, 1339, 3302, 4203, 4525
PspPPI RGGWCCY 1 cut(s) 4525
PspXI VCTCGAGB 2 cut(s) 2442, 2628
PstI CTGCAG 4 cut(s) 1149, 1974, 2049, 4483
PstNI CAGNNNCTG 2 cut(s) 680, 1067
PsyI GACNNNGTC 1 cut(s) 1337
RsaI GTAC 9 cut(s) 832, 1401, 1695, 2206, 3198, 3258, 3544, 3629, 3919
RsaNI GTAC 9 cut(s) 831, 1400, 1694, 2205, 3197, 3257, 3543, 3628, 3918
RseI CAYNNNNRTG 6 cut(s) 2260, 2987, 3102, 4122, 4437, 4606
SacI GAGCTC 1 cut(s) 697
SatI GCNGC 9 cut(s) 620, 744, 796, 1970, 2045, 2396, 3280, 3797, 4482
Sau96I GGNCC 7 cut(s) 87, 114, 123, 1339, 3302, 4203, 4525
ScaI AGTACT 1 cut(s) 3919
SchI GAGTC 8 cut(s) 389, 873, 1643, 1871, 2344, 2879, 4507, 4631
ScrFI CCNGG 7 cut(s) 1263, 1557, 3618, 3645, 3755, 4706, 4856
SfcI CTRYAG 6 cut(s) 561, 1145, 1970, 2045, 3244, 4479
Sfr274I CTCGAG 2 cut(s) 2442, 2628
SfuI TTCGAA 2 cut(s) 633, 1706
SinI GGWCC 3 cut(s) 123, 1339, 4525
SlaI CTCGAG 2 cut(s) 2442, 2628
SmiI ATTTAAAT 2 cut(s) 1482, 2859
SmiMI CAYNNNNRTG 6 cut(s) 2260, 2987, 3102, 4122, 4437, 4606
SmlI CTYRAG 6 cut(s) 1631, 1950, 2442, 2628, 2867, 3422
SmoI CTYRAG 6 cut(s) 1631, 1950, 2442, 2628, 2867, 3422
SphI GCATGC 1 cut(s) 1133
SseBI AGGCCT 2 cut(s) 2687, 3525
SsiI CCGC 7 cut(s) 432, 1855, 1946, 2618, 3010, 3515, 3736
SspI AATATT 3 cut(s) 1303, 3415, 3967
SstI GAGCTC 1 cut(s) 697
StuI AGGCCT 2 cut(s) 2687, 3525
StyD4I CCNGG 7 cut(s) 1261, 1555, 3616, 3643, 3753, 4704, 4854
StyI CCWWGG 4 cut(s) 900, 2246, 3371, 3949
SwaI ATTTAAAT 2 cut(s) 1482, 2859
TaiI ACGT 3 cut(s) 459, 935, 1570
TaqI TCGA 9 cut(s) 82, 633, 867, 1463, 1706, 2443, 2629, 3016, 4471
TatI WGTACW 3 cut(s) 1693, 3196, 3917
TseFI GTSAC 4 cut(s) 727, 928, 2347, 4352
TseI GCWGC 9 cut(s) 619, 743, 795, 1969, 2044, 2395, 3279, 3796, 4481
Tsp45I GTSAC 4 cut(s) 727, 928, 2347, 4352
TspGWI ACGGA 4 cut(s) 1417, 1591, 2823, 3195
Tth111I GACNNNGTC 1 cut(s) 1337
VneI GTGCAC 2 cut(s) 3030, 3552
VpaK11BI GGWCC 3 cut(s) 123, 1339, 4525
VspI ATTAAT 2 cut(s) 1164, 3726
XbaI TCTAGA 1 cut(s) 164
XceI RCATGY 3 cut(s) 1133, 2599, 3328
XcmI CCANNNNNNNNNTGG 1 cut(s) 4056
XhoI CTCGAG 2 cut(s) 2442, 2628
XmiI GTMKAC 2 cut(s) 564, 2145
XmnI GAANNNNTTC 3 cut(s) 2508, 3652, 4723
ZrmI AGTACT 1 cut(s) 3919
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.