Rh7DG470700
ERF Family

Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin- like GTPase superfamily. TrmE GTPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0012258
Physical Location & Seq
Reverse (-)
67254315 .. 67258467
4153 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG470700.1

Sequence Viewer

Length: 1707 bp
ATGGCCTCTCTGCTCTCACCTCTCCGCTTTCGCCATTTTCTTTACACAAGGCCTAAACTGAAACCCCCTTATCCACTCTCCTCCATACCTTCTTCCGCTTCCTTCAAACCCCTTCTCAAACTCCGCCCTCGCTTCCCTCCCTATTTACACTCCTGCCACTGCTCCCTCACCGCCTCTTCCCCACTCGCAGACTCCGACTACAAAACCCAGACGCTGCTTCTCGGCAAAGACGAGCGAGCTGCAGCGCGTGGGTCCCAGCCCCAGCCGCAGCCGGAGAGCGCTAGCACAATCGCGGCCATTGTGACGTCAGTTGGGGGCCCACCCGGCGCGGTTGGGATCGTGCGGCTGTCCGGTCCGTCCGCGGTGGCCGTCGTCGGCCGCGTGTTCCGTCCGAGCAAGAAAAAGAGGAAGAGCAAGAGCTCTGGTGCTGAGCCATGGCGACCCACTAGTCATGTAATCGAATACGGCGTCGTTTTGGACCCCAACGGCAATGTCGTCGACGAGGTTTTAGCTGTGCCAATGTTGGCCCCAAGATCATATACACGTGAAGATGTGGTGGAGCTTCAATGCCATGGGACTCAAGTGTGTTTGAACCGGGTTCTAAGAGCTTGTTTAGAAGCTGGAGCAAGGCTTGCGGAACCAGGGGAGTTTACTCTGCGTGCCTTTCTCAATGGCCGCTTGGACCTGACCCAAGCTGAAAATGTTGATAAACTCATTTCGGCCAAGTCTGTGGCTGCAGCAGATGCAGCACTAGCCGCAATTCAGGGTGGCTTTTCTTCCATGGTAAAATCTGTAAGATTTCAGTGCATTGAGTTGCTTACTGAAATTGAAGCCCGGTTAGATTTTGATGATGAGATGCCGCCGCTTGATACCAATGAAGTTGTGAATAAGATACATTCCATGTCACAAGATGTTGAGAGTGCTCTAGAGACGGCCAATTATGACCAACTTCTGCAATCTGGATTACAGATAGCAATTCTGGGCCGTCCAAATGTTGGGAAATCAAGCCTCCTCAATGCATGGAGCAAAACTGAGAGAGCTATAGTTACAGAAGTTGCTGGGACCACTCGGGATGTGGTTGAAGCCAGTATTACAGTTAATGGTATTCCTGTAACCCTTCTTGATACAGCAGGCATTAGGGAAACCACTGACATAGTGGAGAAAATTGGTGTGGAGAGGTCTGAAGCAGTTGCTATGGGTGCTGATGTCATTATTATGGCAATAAGTGCTCTTGATGGCTGGAGTCCAGAAGACTCTGAACTTCTTGACAGAATCCAATCTAACAAGAAATCGACTGGATCTTCAACCCCAATGATCCTTGTAATTAACAAAATAGATTGTGTTGCATCTGATTGCTTGGAGCGGGTTCAAAAAAATGTCGGTTCTTTCAATAAGCATGTCTTGACATCTGCCATCACTGGTCAAGGAATCCAAGATTTGGAGAAGGCAATATTAGATATTGTTGGTCTCAACAAATTTCCTGCAGGGAGTCGAAGGTGGACTGTGAACCAGAGACAATGCGAGCAGCTTGTCCGAACCAAAGAGGCTCTTGCAAGGTTGAAATCCTCTATAGAGGAGGAAATTCCTTTTGACTTCTGGACAATTGATTTGAGGGAAGCTGCAATGGCCCTTGGCCAAATAAGTGGAGAAGACATTTCGGAGGAGGTTTTGACCAATATTTTTGGCAAGTTCTGCATTGGTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MMR_HSR1 MnmE_helical TrmE_N

Protein Analysis

568

Amino Acids

61.51

Weight (kDa)

6.1

Isoelectric Point (pI)

44.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TrmE_N PF10396 96 - 226 3.4e-37 GTP-binding protein TrmE N-terminus
MnmE_helical PF12631 229 - 565 1.8e-49 MnmE helical domain
MMR_HSR1 PF01926 323 - 444 1.5e-24 50S ribosome-binding GTPase
FeoB_N PF02421 323 - 484 1.3e-08 Ferrous iron transport protein B
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012258)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78010
fragaria_vesca FvH4_5g38010 FvH4_5g38010
malus_domestica MD08G1177300.v1.1 MD15G1355000.v1.1
prunus_persica Prupe.1G511900_v2.0.a1
pyrus_communis pycom08g15220
rosa_chinensis RchiOBHm_Chr7g0239951
rosa_laevigata RLG00000000715
rosa_multiflora Rmu_sc0000540.1_g000022 Rmu_sc0006825.1_g000001 Rmu_sc0035965.1_g000001
rosa_roxburghii Rroxscaffold_3G00220600
rosa_rugosa Rorug07G0331400
rosa_samantha Rh7AG485100 Rh7BG458100 Rh7CG502400 Rh7DG470700
rosa_wichuraiana Rw7G040690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 447
AatII GACGTC 1 cut(s) 308
AccB7I CCANNNNNTGG 2 cut(s) 995, 1438
AccBSI CCGCTC 1 cut(s) 1363
AccI GTMKAC 1 cut(s) 498
AccII CGCG 5 cut(s) 247, 293, 329, 362, 381
AclWI GGATC 3 cut(s) 344, 1306, 1309
AcoI YGGCCR 7 cut(s) 294, 366, 376, 673, 720, 933, 1633
AcsI RAATTY 2 cut(s) 1475, 1581
AcuI CTGAAG 1 cut(s) 1203
AcvI CACGTG 1 cut(s) 545
AcyI GRCGYC 2 cut(s) 305, 468
AfeI AGCGCT 1 cut(s) 280
AfiI CCNNNNNNNGG 2 cut(s) 995, 1438
AflIII ACRYGT 1 cut(s) 542
AgsI TTSAA 9 cut(s) 106, 566, 592, 830, 1082, 1305, 1370, 1390, 1561
AhlI ACTAGT 1 cut(s) 446
AjnI CCWGG 1 cut(s) 640
Alw21I GWGCWC 3 cut(s) 422, 925, 1231
Alw26I GTCTC 3 cut(s) 923, 1472, 1507
AlwI GGATC 3 cut(s) 344, 1306, 1309
AlwNI CAGNNNCTG 1 cut(s) 214
Ama87I CYCGRG 1 cut(s) 1068
Aor51HI AGCGCT 1 cut(s) 280
ApaI GGGCCC 1 cut(s) 320
ApeKI GCWGC 9 cut(s) 214, 239, 242, 268, 734, 737, 746, 1525, 1619
ApoI RAATTY 2 cut(s) 1475, 1581
ArsI GACNNNNNNTTYG 4 cut(s) 453, 485, 1591, 1623
AspLEI GCGC 3 cut(s) 247, 281, 329
AsuC2I CCSGG 3 cut(s) 324, 596, 835
AsuHPI GGTGA 2 cut(s) 9, 160
AsuNHI GCTAGC 1 cut(s) 281
AvaI CYCGRG 1 cut(s) 1068
AvaII GGWCC 5 cut(s) 252, 353, 478, 682, 1062
BaeGI GKGCMC 1 cut(s) 320
BalI TGGCCA 1 cut(s) 1635
BanII GRGCYC 2 cut(s) 320, 422
BbrPI CACGTG 1 cut(s) 545
BbsI GAAGAC 2 cut(s) 1257, 1656
Bbv12I GWGCWC 3 cut(s) 422, 925, 1231
BbvI GCAGC 9 cut(s) 201, 226, 254, 280, 721, 749, 758, 1537, 1606
BccI CCATC 2 cut(s) 1229, 1421
BceAI ACGGC 5 cut(s) 353, 481, 502, 948, 969
BcgI CGANNNNNNTGC 4 cut(s) 221, 255, 478, 512
BciT130I CCWGG 1 cut(s) 642
BcnI CCSGG 3 cut(s) 324, 596, 835
BcoDI GTCTC 3 cut(s) 923, 1472, 1507
BcuI ACTAGT 1 cut(s) 446
BfaI CTAG 4 cut(s) 282, 447, 752, 926
BfmI CTRYAG 5 cut(s) 240, 735, 1041, 1482, 1569
BfoI RGCGCY 1 cut(s) 282
BglI GCCNNNNNGGC 1 cut(s) 324
BlpI GCTNAGC 1 cut(s) 429
Bme1390I CCNGG 4 cut(s) 324, 596, 642, 835
Bme18I GGWCC 5 cut(s) 252, 353, 478, 682, 1062
BmeT110I CYCGRG 1 cut(s) 1068
BmiI GGNNCC 8 cut(s) 253, 254, 317, 318, 480, 528, 639, 1063
BmrFI CCNGG 4 cut(s) 324, 596, 642, 835
BmsI GCATC 3 cut(s) 733, 846, 1355
BmtI GCTAGC 1 cut(s) 285
BpiI GAAGAC 2 cut(s) 1257, 1656
BpmI CTGGAG 2 cut(s) 642, 1261
Bpu1102I GCTNAGC 1 cut(s) 429
BpuEI CTTGAG 1 cut(s) 564
BpuMI CCSGG 3 cut(s) 324, 596, 835
BsaAI YACGTR 1 cut(s) 545
BsaHI GRCGYC 2 cut(s) 305, 468
BsaI GGTCTC 1 cut(s) 1472
BsaJI CCNNGG 6 cut(s) 360, 434, 571, 641, 780, 1630
BsaWI WCCGGW 1 cut(s) 350
Bsc4I CCNNNNNNNGG 2 cut(s) 995, 1438
Bse1I ACTGG 3 cut(s) 1086, 1300, 1423
Bse3DI GCAATG 2 cut(s) 496, 1629
BseBI CCWGG 1 cut(s) 642
BseDI CCNNGG 6 cut(s) 360, 434, 571, 641, 780, 1630
BseGI GGATG 1 cut(s) 1078
BseLI CCNNNNNNNGG 2 cut(s) 995, 1438
BseMI GCAATG 2 cut(s) 496, 1629
BseMII CTCAG 2 cut(s) 420, 1023
BseNI ACTGG 3 cut(s) 1086, 1300, 1423
BseRI GAGGAG 4 cut(s) 70, 1001, 1589, 1676
BseSI GKGCMC 1 cut(s) 320
BseX3I CGGCCG 1 cut(s) 376
BseXI GCAGC 9 cut(s) 201, 226, 254, 280, 721, 749, 758, 1537, 1606
BseYI CCCAGC 3 cut(s) 255, 261, 1058
Bsh1236I CGCG 5 cut(s) 247, 293, 329, 362, 381
Bsh1285I CGRYCG 1 cut(s) 379
BsiEI CGRYCG 1 cut(s) 379
BsiHKAI GWGCWC 3 cut(s) 422, 925, 1231
BsiHKCI CYCGRG 1 cut(s) 1068
BsiSI CCGG 5 cut(s) 272, 324, 351, 595, 835
BslFI GGGAC 3 cut(s) 238, 589, 1075
BslI CCNNNNNNNGG 2 cut(s) 995, 1438
BsmAI GTCTC 3 cut(s) 923, 1472, 1507
BsmBI CGTCTC 1 cut(s) 923
BsmFI GGGAC 3 cut(s) 238, 589, 1075
Bso31I GGTCTC 1 cut(s) 1472
BsoBI CYCGRG 1 cut(s) 1068
Bsp120I GGGCCC 1 cut(s) 316
Bsp1286I GDGCHC 4 cut(s) 320, 422, 925, 1231
Bsp143I GATC 4 cut(s) 336, 533, 1298, 1314
Bsp1720I GCTNAGC 1 cut(s) 429
Bsp19I CCATGG 3 cut(s) 434, 571, 780
BspCNI CTCAG 2 cut(s) 421, 1024
BspFNI CGCG 5 cut(s) 247, 293, 329, 362, 381
BspLI GGNNCC 8 cut(s) 253, 254, 317, 318, 480, 528, 639, 1063
BspMAI CTGCAG 3 cut(s) 244, 739, 1486
BspOI GCTAGC 1 cut(s) 285
BspPI GGATC 3 cut(s) 344, 1306, 1309
BspQI GCTCTTC 1 cut(s) 404
BspTNI GGTCTC 1 cut(s) 1472
BsrBI CCGCTC 1 cut(s) 1363
BsrDI GCAATG 2 cut(s) 496, 1629
BsrI ACTGG 3 cut(s) 1086, 1300, 1423
BssECI CCNNGG 6 cut(s) 360, 434, 571, 641, 780, 1630
BssMI GATC 4 cut(s) 336, 533, 1298, 1314
BssNI GRCGYC 2 cut(s) 305, 468
BssT1I CCWWGG 4 cut(s) 434, 571, 780, 1630
Bst2UI CCWGG 1 cut(s) 642
Bst4CI ACNGT 2 cut(s) 1096, 1504
Bst6I CTCTTC 2 cut(s) 181, 404
BstACI GRCGYC 2 cut(s) 305, 468
BstAPI GCANNNNNTGC 4 cut(s) 632, 743, 1226, 1692
BstBAI YACGTR 1 cut(s) 545
BstC8I GCNNGC 6 cut(s) 237, 283, 633, 660, 1132, 1523
BstDEI CTNAG 3 cut(s) 429, 602, 1032
BstDSI CCRYGG 4 cut(s) 360, 434, 571, 780
BstF5I GGATG 1 cut(s) 1078
BstFNI CGCG 5 cut(s) 247, 293, 329, 362, 381
BstH2I RGCGCY 1 cut(s) 282
BstHHI GCGC 3 cut(s) 247, 281, 329
BstKTI GATC 4 cut(s) 339, 536, 1301, 1317
BstMAI GTCTC 3 cut(s) 923, 1472, 1507
BstMBI GATC 4 cut(s) 336, 533, 1298, 1314
BstMCI CGRYCG 1 cut(s) 379
BstNI CCWGG 1 cut(s) 642
BstNSI RCATGY 1 cut(s) 1400
BstSCI CCNGG 4 cut(s) 322, 594, 640, 833
BstSFI CTRYAG 5 cut(s) 240, 735, 1041, 1482, 1569
BstSLI GKGCMC 1 cut(s) 320
BstUI CGCG 5 cut(s) 247, 293, 329, 362, 381
BstV1I GCAGC 9 cut(s) 201, 226, 254, 280, 721, 749, 758, 1537, 1606
BstV2I GAAGAC 2 cut(s) 1257, 1656
BstX2I RGATCY 1 cut(s) 1298
BstXI CCANNNNNNTGG 2 cut(s) 730, 1643
BstYI RGATCY 1 cut(s) 1298
BstZI CGGCCG 1 cut(s) 376
BtgI CCRYGG 4 cut(s) 360, 434, 571, 780
BtsCI GGATG 1 cut(s) 1078
BtsI GCAGTG 1 cut(s) 157
BtsIMutI CAGTG 4 cut(s) 157, 809, 1146, 1416
Cac8I GCNNGC 6 cut(s) 237, 283, 633, 660, 1132, 1523
CaiI CAGNNNCTG 1 cut(s) 214
CfoI GCGC 3 cut(s) 247, 281, 329
Cfr42I CCGCGG 1 cut(s) 363
CpoI CGGWCCG 1 cut(s) 353
CseI GACGC 2 cut(s) 220, 457
CspI CGGWCCG 1 cut(s) 353
CviAII CATG 7 cut(s) 435, 452, 572, 781, 901, 1020, 1397
DdeI CTNAG 3 cut(s) 429, 602, 1032
DpnI GATC 4 cut(s) 338, 535, 1300, 1316
DpnII GATC 4 cut(s) 336, 533, 1298, 1314
DrdI GACNNNNNNGTC 1 cut(s) 447
DseDI GACNNNNNNGTC 1 cut(s) 447
EaeI YGGCCR 7 cut(s) 294, 366, 376, 673, 720, 933, 1633
EagI CGGCCG 1 cut(s) 376
Eam1104I CTCTTC 2 cut(s) 181, 404
EarI CTCTTC 2 cut(s) 181, 404
EciI GGCGGA 1 cut(s) 113
Ecl136II GAGCTC 1 cut(s) 420
EclXI CGGCCG 1 cut(s) 376
Eco130I CCWWGG 4 cut(s) 434, 571, 780, 1630
Eco147I AGGCCT 1 cut(s) 52
Eco24I GRGCYC 2 cut(s) 320, 422
Eco31I GGTCTC 1 cut(s) 1472
Eco47I GGWCC 5 cut(s) 252, 353, 478, 682, 1062
Eco47III AGCGCT 1 cut(s) 280
Eco52I CGGCCG 1 cut(s) 376
Eco53kI GAGCTC 1 cut(s) 420
Eco57I CTGAAG 1 cut(s) 1203
Eco72I CACGTG 1 cut(s) 545
Eco88I CYCGRG 1 cut(s) 1068
EcoICRI GAGCTC 1 cut(s) 420
EcoO109I RGGNCCY 2 cut(s) 252, 316
EcoRII CCWGG 1 cut(s) 640
EcoT14I CCWWGG 4 cut(s) 434, 571, 780, 1630
EcoT22I ATGCAT 1 cut(s) 1021
EcoT38I GRGCYC 2 cut(s) 320, 422
ErhI CCWWGG 4 cut(s) 434, 571, 780, 1630
Esp3I CGTCTC 1 cut(s) 923
FaeI CATG 7 cut(s) 438, 455, 575, 784, 904, 1023, 1400
FalI AAGNNNNNCTT 4 cut(s) 1385, 1417, 1533, 1565
FaqI GGGAC 3 cut(s) 238, 589, 1075
FatI CATG 7 cut(s) 434, 451, 571, 780, 900, 1019, 1396
FauI CCCGC 1 cut(s) 1356
FblI GTMKAC 1 cut(s) 498
FokI GGATG 1 cut(s) 1085
FriOI GRGCYC 2 cut(s) 320, 422
FspBI CTAG 4 cut(s) 282, 447, 752, 926
GlaI GCGC 3 cut(s) 246, 280, 328
GsaI CCCAGC 3 cut(s) 259, 265, 1062
GsuI CTGGAG 2 cut(s) 642, 1261
HaeII RGCGCY 1 cut(s) 282
HapII CCGG 5 cut(s) 272, 324, 351, 595, 835
HgaI GACGC 2 cut(s) 220, 457
HhaI GCGC 3 cut(s) 247, 281, 329
Hin1I GRCGYC 2 cut(s) 305, 468
Hin1II CATG 7 cut(s) 438, 455, 575, 784, 904, 1023, 1400
Hin6I GCGC 3 cut(s) 245, 279, 327
HinP1I GCGC 3 cut(s) 245, 279, 327
HincII GTYRAC 1 cut(s) 499
HindII GTYRAC 1 cut(s) 499
HinfI GANTC 7 cut(s) 191, 577, 1243, 1253, 1272, 1428, 1489
HpaII CCGG 5 cut(s) 272, 324, 351, 595, 835
HphI GGTGA 2 cut(s) 9, 160
Hpy166II GTNNAC 4 cut(s) 499, 651, 1500, 1507
Hpy188I TCNGA 7 cut(s) 196, 393, 1183, 1258, 1351, 1535, 1660
Hpy188III TCNNGA 9 cut(s) 926, 960, 1070, 1121, 1232, 1247, 1265, 1402, 1597
Hpy8I GTNNAC 4 cut(s) 499, 651, 1500, 1507
Hpy99I CGWCG 5 cut(s) 374, 377, 473, 500, 503
HpyAV CCTTC 6 cut(s) 99, 112, 122, 1127, 1438, 1488
HpyCH4III ACNGT 2 cut(s) 1096, 1504
HpyCH4IV ACGT 2 cut(s) 305, 544
HpyF3I CTNAG 3 cut(s) 429, 602, 1032
HpySE526I ACGT 2 cut(s) 305, 544
Hsp92I GRCGYC 2 cut(s) 305, 468
Hsp92II CATG 7 cut(s) 438, 455, 575, 784, 904, 1023, 1400
HspAI GCGC 3 cut(s) 245, 279, 327
KflI GGGWCCC 1 cut(s) 252
KspI CCGCGG 1 cut(s) 363
Kzo9I GATC 4 cut(s) 336, 533, 1298, 1314
LguI GCTCTTC 1 cut(s) 404
LmnI GCTCC 5 cut(s) 167, 559, 623, 1023, 1360
Lsp1109I GCAGC 9 cut(s) 201, 226, 254, 280, 721, 749, 758, 1537, 1606
LweI GCATC 3 cut(s) 733, 846, 1355
MaeI CTAG 4 cut(s) 282, 447, 752, 926
MaeII ACGT 2 cut(s) 305, 544
MaeIII GTNAC 4 cut(s) 301, 903, 1045, 1111
MalI GATC 4 cut(s) 338, 535, 1300, 1316
MbiI CCGCTC 1 cut(s) 1363
MboI GATC 4 cut(s) 336, 533, 1298, 1314
MboII GAAGA 8 cut(s) 84, 168, 421, 560, 768, 1262, 1293, 1661
MfeI CAATTG 1 cut(s) 1602
MflI RGATCY 1 cut(s) 1298
MhlI GDGCHC 4 cut(s) 320, 422, 925, 1231
MlsI TGGCCA 1 cut(s) 1635
MluCI AATT 9 cut(s) 759, 825, 937, 975, 1164, 1323, 1475, 1581, 1602
MluNI TGGCCA 1 cut(s) 1635
MlyI GAGTC 5 cut(s) 185, 571, 1247, 1252, 1498
MmeI TCCRAC 1 cut(s) 219
Mox20I TGGCCA 1 cut(s) 1635
Mph1103I ATGCAT 1 cut(s) 1021
MscI TGGCCA 1 cut(s) 1635
MseI TTAA 2 cut(s) 1098, 1326
MslI CAYNNNNRTG 1 cut(s) 1214
Msp20I TGGCCA 1 cut(s) 1635
MspA1I CMGCKG 1 cut(s) 362
MspI CCGG 5 cut(s) 272, 324, 351, 595, 835
MspR9I CCNGG 4 cut(s) 324, 596, 642, 835
MunI CAATTG 1 cut(s) 1602
MvaI CCWGG 1 cut(s) 642
MvnI CGCG 5 cut(s) 247, 293, 329, 362, 381
NciI CCSGG 3 cut(s) 324, 596, 835
NcoI CCATGG 3 cut(s) 434, 571, 780
NdeII GATC 4 cut(s) 336, 533, 1298, 1314
NheI GCTAGC 1 cut(s) 281
NlaIII CATG 7 cut(s) 438, 455, 575, 784, 904, 1023, 1400
NlaIV GGNNCC 8 cut(s) 253, 254, 317, 318, 480, 528, 639, 1063
NmeAIII GCCGAG 1 cut(s) 201
NmuCI GTSAC 2 cut(s) 301, 903
NsiI ATGCAT 1 cut(s) 1021
NspI RCATGY 1 cut(s) 1400
PceI AGGCCT 1 cut(s) 52
PciSI GCTCTTC 1 cut(s) 404
PcsI WCGNNNNNNNCGW 5 cut(s) 192, 228, 378, 465, 492
PfeI GAWTC 2 cut(s) 1272, 1428
PflMI CCANNNNNTGG 2 cut(s) 995, 1438
PleI GAGTC 5 cut(s) 185, 571, 1247, 1251, 1497
PmaCI CACGTG 1 cut(s) 545
PmlI CACGTG 1 cut(s) 545
PpsI GAGTC 5 cut(s) 185, 571, 1247, 1251, 1497
Ppu21I YACGTR 1 cut(s) 545
PpuMI RGGWCCY 1 cut(s) 252
Psp124BI GAGCTC 1 cut(s) 422
Psp5II RGGWCCY 1 cut(s) 252
Psp6I CCWGG 1 cut(s) 640
PspCI CACGTG 1 cut(s) 545
PspFI CCCAGC 3 cut(s) 255, 261, 1058
PspGI CCWGG 1 cut(s) 640
PspN4I GGNNCC 8 cut(s) 253, 254, 317, 318, 480, 528, 639, 1063
PspOMI GGGCCC 1 cut(s) 316
PspPPI RGGWCCY 1 cut(s) 252
PstI CTGCAG 3 cut(s) 244, 739, 1486
PstNI CAGNNNCTG 1 cut(s) 214
PsuI RGATCY 1 cut(s) 1298
RseI CAYNNNNRTG 1 cut(s) 1214
Rsr2I CGGWCCG 1 cut(s) 353
RsrII CGGWCCG 1 cut(s) 353
SacI GAGCTC 1 cut(s) 422
SacII CCGCGG 1 cut(s) 363
SalI GTCGAC 1 cut(s) 497
SapI GCTCTTC 1 cut(s) 404
SaqAI TTAA 2 cut(s) 1098, 1326
Sau3AI GATC 4 cut(s) 336, 533, 1298, 1314
SbfI CCTGCAGG 1 cut(s) 1486
SchI GAGTC 5 cut(s) 185, 571, 1247, 1252, 1498
ScrFI CCNGG 4 cut(s) 324, 596, 642, 835
SdaI CCTGCAGG 1 cut(s) 1486
SduI GDGCHC 4 cut(s) 320, 422, 925, 1231
SfaNI GCATC 3 cut(s) 733, 846, 1355
SfcI CTRYAG 5 cut(s) 240, 735, 1041, 1482, 1569
Sfr303I CCGCGG 1 cut(s) 363
SgrBI CCGCGG 1 cut(s) 363
SgrDI CGTCGACG 1 cut(s) 497
SinI GGWCC 5 cut(s) 252, 353, 478, 682, 1062
SmiMI CAYNNNNRTG 1 cut(s) 1214
SmlI CTYRAG 1 cut(s) 579
SmoI CTYRAG 1 cut(s) 579
SpeI ACTAGT 1 cut(s) 446
Sse8387I CCTGCAGG 1 cut(s) 1486
Sse9I AATT 9 cut(s) 759, 825, 937, 975, 1164, 1323, 1475, 1581, 1602
SseBI AGGCCT 1 cut(s) 52
SspI AATATT 2 cut(s) 1452, 1678
SspMI CTAG 4 cut(s) 282, 447, 752, 926
SstI GAGCTC 1 cut(s) 422
StuI AGGCCT 1 cut(s) 52
StyD4I CCNGG 4 cut(s) 322, 594, 640, 833
StyI CCWWGG 4 cut(s) 434, 571, 780, 1630
TaaI ACNGT 2 cut(s) 1096, 1504
TaiI ACGT 2 cut(s) 308, 547
TaqI TCGA 4 cut(s) 459, 498, 1292, 1492
TasI AATT 9 cut(s) 759, 825, 937, 975, 1164, 1323, 1475, 1581, 1602
TauI GCSGC 8 cut(s) 268, 296, 346, 381, 678, 758, 862, 865
TfiI GAWTC 2 cut(s) 1272, 1428
Tru1I TTAA 2 cut(s) 1098, 1326
Tru9I TTAA 2 cut(s) 1098, 1326
TscAI CASTG 4 cut(s) 164, 809, 1153, 1423
TseFI GTSAC 2 cut(s) 301, 903
TseI GCWGC 9 cut(s) 214, 239, 242, 268, 734, 737, 746, 1525, 1619
Tsp45I GTSAC 2 cut(s) 301, 903
TspDTI ATGAA 1 cut(s) 891
TspGWI ACGGA 2 cut(s) 345, 377
TspRI CASTG 4 cut(s) 164, 809, 1153, 1423
Van91I CCANNNNNTGG 2 cut(s) 995, 1438
VpaK11BI GGWCC 5 cut(s) 252, 353, 478, 682, 1062
XapI RAATTY 2 cut(s) 1475, 1581
XbaI TCTAGA 1 cut(s) 925
XceI RCATGY 1 cut(s) 1400
XcmI CCANNNNNNNNNTGG 2 cut(s) 1072, 1153
XmiI GTMKAC 1 cut(s) 498
XspI CTAG 4 cut(s) 282, 447, 752, 926
ZraI GACGTC 1 cut(s) 306
Zsp2I ATGCAT 1 cut(s) 1021
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.