Rmu_sc0000540.1_g000022
ERF Family

Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin- like GTPase superfamily. TrmE GTPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000540.1
OG0012258
Physical Location & Seq
Forward (+)
107711 .. 112683
4973 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000540.1_g000022.1.cds

Sequence Viewer

Length: 2094 bp
atggcctcgctgctctcacctctccgctttcgccattttctttacacgaggcctaaactaaaactgaaacccccttatctaatctctgccgcctctttcaaacccctcctcaaactccgccctcgctcccctccctatttacactcctgccactgctccttcaccgcctcttccccactcgcagactccggctacaaaacccagacgccgcttctcggcagagacgagcgagctgccgtagtgagtgggtcccagccgcagccggagagcgctagcacaatcgcggccattgtgacgtcagttgggggcccacccggcgcggttgggatcgtgcggctgtccggcccgtccgcggtggccgtcgtcggccgcgtgttccgtccgagcaagaaaaggagaaagagcaagagctctggtgctgagccatggcgacccactagtcatgtggtcgaatacggcgtcgttttggaccccaacggcaatgtcgtcgacgaggttttagctgtgccaatgttggccccaagatcatatacacgtgaagatgtggtggagcttcaatgccatgggactcaagtgtgtctgaaccgggttctcagagcttgtttagaagctggagcaaggcttgcggaaccaggggagttcactctgcgtgcctttctcaatggccgcttggacctgacccaagctgaaaatgttgataacctcatttcggccaagtctgtggctgcagcagatgcagcgctagcggcaattcagggtggcttttcttccatggtaaaatccgtgagatttcagtgcattgagttgcttactgaaattgaagcccggttagattttgatgatgagatgccgccgcttgataccaatgaagttgtgaataaaatacattccatgtcacaagatgttgagagtgctctagagacggccaattatgaccaacttctgcaatctggattacagatagcaattctgggccgtccaaatgttgggaaatcaagcctcctcaatgcatggagcaaaactgagagagctatagttacagaagttgctgggaccactcgggatgtggttgaagccagtattacagttaatggtattcctgtaacccttcttgatacagcaggcatcagggaaaccactgacatagtggagaaaattggtgtggagaggtctgaagcagttgctatgggtgctgatgtcattattatggcaataagtgctcttgatggctggagtccagaagactctgaacttcttgacagaatccaatctaacaagaaatcaactggatcttcaaccccaatgatccttgtagttaacaaaatagattgtgttgcatctgattgcttggagtgggttcaaaaaaatgttggttctttcaataagcatgtcttgacatctgccatcactggtcaaggaatccaagatttggagaaggcaatattagatattgttggtctcaacaaatttcctgcagggagtcgaaggtggactgtgaatcagagacaatgcgagcagcttgtccgaaccaaagaggctcttgcaaggttgaaatcctctatagaggaggaaattccttttgacttctggacgattgatttgagggaagctgcaatggcccttggccaaataagtggagaagacatttcagaggaggttttgaccaatatttttggcaagttctgcattggaaaagaactgacaaaggaggaactcaaggagaggcaaaaggtaaggaaagccaaggagaaggagaagaggaaaagaatagaacaggagaaagagaaggagaagaagaggctggaacaagaaatgaaaaagaagcaagagaaagagaagaagaaacgcgaagagttgaaacgaaaagaaaaggagaaggcgaagatgaagatgatcaaggagaggcaaaaggtaaggaaagccaaggagaaggagaagaggaaaagaatagaacaggagaaagagaaggagaagaagaggctggaacaagagatgaaaaagaagcaagagaaagagaagaagaaacgcgaagagttgaaacgaaaagaaaaggagaaggcgaagatgaagatgaagcacaagatgaagaaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MMR_HSR1 MnmE_helical TrmE_N

Protein Analysis

697

Amino Acids

78.07

Weight (kDa)

9.48

Isoelectric Point (pI)

50.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012258)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78010
fragaria_vesca FvH4_5g38010 FvH4_5g38010
malus_domestica MD08G1177300.v1.1 MD15G1355000.v1.1
prunus_persica Prupe.1G511900_v2.0.a1
pyrus_communis pycom08g15220
rosa_chinensis RchiOBHm_Chr7g0239951
rosa_laevigata RLG00000000715
rosa_multiflora Rmu_sc0000540.1_g000022 Rmu_sc0006825.1_g000001 Rmu_sc0035965.1_g000001
rosa_roxburghii Rroxscaffold_3G00220600
rosa_rugosa Rorug07G0331400
rosa_samantha Rh7AG485100 Rh7BG458100 Rh7CG502400 Rh7DG470700
rosa_wichuraiana Rw7G040690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 438
AatII GACGTC 1 cut(s) 299
AccB7I CCANNNNNTGG 2 cut(s) 986, 1429
AccI GTMKAC 1 cut(s) 489
AccII CGCG 6 cut(s) 284, 320, 353, 372, 1848, 2028
AclWI GGATC 3 cut(s) 335, 1297, 1300
AcoI YGGCCR 7 cut(s) 285, 357, 367, 664, 711, 924, 1624
AcsI RAATTY 2 cut(s) 1466, 1572
AcuI CTGAAG 1 cut(s) 1194
AcvI CACGTG 1 cut(s) 536
AcyI GRCGYC 3 cut(s) 206, 296, 459
AfeI AGCGCT 2 cut(s) 271, 741
AfiI CCNNNNNNNGG 5 cut(s) 215, 352, 709, 986, 1429
AflIII ACRYGT 1 cut(s) 533
AhlI ACTAGT 1 cut(s) 437
AjnI CCWGG 1 cut(s) 631
Alw21I GWGCWC 3 cut(s) 413, 916, 1222
Alw26I GTCTC 4 cut(s) 216, 914, 1463, 1498
AlwI GGATC 3 cut(s) 335, 1297, 1300
Ama87I CYCGRG 1 cut(s) 1059
Aor51HI AGCGCT 2 cut(s) 271, 741
ApaI GGGCCC 1 cut(s) 311
ApeKI GCWGC 8 cut(s) 10, 233, 259, 725, 728, 737, 1516, 1610
ApoI RAATTY 2 cut(s) 1466, 1572
ArsI GACNNNNNNTTYG 4 cut(s) 444, 476, 1582, 1614
AspLEI GCGC 3 cut(s) 272, 320, 742
AsuC2I CCSGG 3 cut(s) 315, 587, 826
AsuHPI GGTGA 2 cut(s) 9, 154
AsuNHI GCTAGC 2 cut(s) 272, 742
AvaI CYCGRG 1 cut(s) 1059
AvaII GGWCC 4 cut(s) 249, 469, 673, 1053
BaeGI GKGCMC 1 cut(s) 311
BalI TGGCCA 1 cut(s) 1626
BanII GRGCYC 2 cut(s) 311, 413
BauI CACGAG 1 cut(s) 46
BbrPI CACGTG 1 cut(s) 536
BbsI GAAGAC 2 cut(s) 1248, 1647
Bbv12I GWGCWC 3 cut(s) 413, 916, 1222
BbvI GCAGC 7 cut(s) 220, 271, 712, 740, 749, 1528, 1597
BccI CCATC 2 cut(s) 1220, 1412
BceAI ACGGC 6 cut(s) 221, 344, 472, 493, 939, 960
BcgI CGANNNNNNTGC 4 cut(s) 215, 249, 469, 503
BciT130I CCWGG 1 cut(s) 633
BclI TGATCA 1 cut(s) 1893
BcnI CCSGG 3 cut(s) 315, 587, 826
BcoDI GTCTC 4 cut(s) 216, 914, 1463, 1498
BcuI ACTAGT 1 cut(s) 437
BfaI CTAG 4 cut(s) 273, 438, 743, 917
BfmI CTRYAG 4 cut(s) 726, 1032, 1473, 1560
BfoI RGCGCY 2 cut(s) 273, 743
BglI GCCNNNNNGGC 1 cut(s) 315
BlpI GCTNAGC 1 cut(s) 420
Bme1390I CCNGG 4 cut(s) 315, 587, 633, 826
Bme18I GGWCC 4 cut(s) 249, 469, 673, 1053
BmeT110I CYCGRG 1 cut(s) 1059
BmiI GGNNCC 8 cut(s) 250, 251, 308, 309, 471, 519, 630, 1054
BmrFI CCNGG 4 cut(s) 315, 587, 633, 826
BmsI GCATC 4 cut(s) 724, 837, 1134, 1346
BmtI GCTAGC 2 cut(s) 276, 746
BpiI GAAGAC 2 cut(s) 1248, 1647
BpmI CTGGAG 2 cut(s) 633, 1252
Bpu1102I GCTNAGC 1 cut(s) 420
BpuEI CTTGAG 2 cut(s) 555, 1700
BpuMI CCSGG 3 cut(s) 315, 587, 826
BsaAI YACGTR 1 cut(s) 536
BsaHI GRCGYC 3 cut(s) 206, 296, 459
BsaI GGTCTC 1 cut(s) 1463
BsaJI CCNNGG 8 cut(s) 351, 425, 562, 632, 771, 1621, 1743, 1923
Bsc4I CCNNNNNNNGG 5 cut(s) 215, 352, 709, 986, 1429
Bse1I ACTGG 3 cut(s) 1077, 1291, 1414
Bse3DI GCAATG 2 cut(s) 487, 1620
BseBI CCWGG 1 cut(s) 633
BseDI CCNNGG 8 cut(s) 351, 425, 562, 632, 771, 1621, 1743, 1923
BseGI GGATG 1 cut(s) 1069
BseLI CCNNNNNNNGG 5 cut(s) 215, 352, 709, 986, 1429
BseMI GCAATG 2 cut(s) 487, 1620
BseMII CTCAG 3 cut(s) 411, 607, 1014
BseNI ACTGG 3 cut(s) 1077, 1291, 1414
BseRI GAGGAG 4 cut(s) 98, 992, 1580, 1667
BseSI GKGCMC 1 cut(s) 311
BseX3I CGGCCG 1 cut(s) 367
BseXI GCAGC 7 cut(s) 220, 271, 712, 740, 749, 1528, 1597
BseYI CCCAGC 2 cut(s) 252, 1049
Bsh1236I CGCG 6 cut(s) 284, 320, 353, 372, 1848, 2028
Bsh1285I CGRYCG 1 cut(s) 370
BsiEI CGRYCG 1 cut(s) 370
BsiHKAI GWGCWC 3 cut(s) 413, 916, 1222
BsiHKCI CYCGRG 1 cut(s) 1059
BsiSI CCGG 6 cut(s) 189, 263, 315, 342, 586, 826
BslFI GGGAC 3 cut(s) 235, 580, 1066
BslI CCNNNNNNNGG 5 cut(s) 215, 352, 709, 986, 1429
BsmAI GTCTC 4 cut(s) 216, 914, 1463, 1498
BsmBI CGTCTC 2 cut(s) 216, 914
BsmFI GGGAC 3 cut(s) 235, 580, 1066
Bso31I GGTCTC 1 cut(s) 1463
BsoBI CYCGRG 1 cut(s) 1059
Bsp120I GGGCCC 1 cut(s) 307
Bsp1286I GDGCHC 4 cut(s) 311, 413, 916, 1222
Bsp143I GATC 5 cut(s) 327, 524, 1289, 1305, 1893
Bsp1720I GCTNAGC 1 cut(s) 420
Bsp19I CCATGG 3 cut(s) 425, 562, 771
BspCNI CTCAG 3 cut(s) 412, 606, 1015
BspFNI CGCG 6 cut(s) 284, 320, 353, 372, 1848, 2028
BspLI GGNNCC 8 cut(s) 250, 251, 308, 309, 471, 519, 630, 1054
BspMAI CTGCAG 2 cut(s) 730, 1477
BspOI GCTAGC 2 cut(s) 276, 746
BspPI GGATC 3 cut(s) 335, 1297, 1300
BspTNI GGTCTC 1 cut(s) 1463
BsrDI GCAATG 2 cut(s) 487, 1620
BsrI ACTGG 3 cut(s) 1077, 1291, 1414
BssECI CCNNGG 8 cut(s) 351, 425, 562, 632, 771, 1621, 1743, 1923
BssMI GATC 5 cut(s) 327, 524, 1289, 1305, 1893
BssNI GRCGYC 3 cut(s) 206, 296, 459
BssSI CACGAG 1 cut(s) 46
BssT1I CCWWGG 6 cut(s) 425, 562, 771, 1621, 1743, 1923
Bst2BI CACGAG 1 cut(s) 46
Bst2UI CCWGG 1 cut(s) 633
Bst4CI ACNGT 2 cut(s) 1087, 1495
Bst6I CTCTTC 7 cut(s) 175, 1751, 1790, 1845, 1931, 1970, 2025
BstACI GRCGYC 3 cut(s) 206, 296, 459
BstAPI GCANNNNNTGC 4 cut(s) 623, 734, 1217, 1683
BstBAI YACGTR 1 cut(s) 536
BstC8I GCNNGC 7 cut(s) 231, 274, 624, 651, 744, 1123, 1514
BstDEI CTNAG 3 cut(s) 420, 593, 1023
BstDSI CCRYGG 4 cut(s) 351, 425, 562, 771
BstF5I GGATG 1 cut(s) 1069
BstFNI CGCG 6 cut(s) 284, 320, 353, 372, 1848, 2028
BstH2I RGCGCY 2 cut(s) 273, 743
BstHHI GCGC 3 cut(s) 272, 320, 742
BstKTI GATC 5 cut(s) 330, 527, 1292, 1308, 1896
BstMAI GTCTC 4 cut(s) 216, 914, 1463, 1498
BstMBI GATC 5 cut(s) 327, 524, 1289, 1305, 1893
BstMCI CGRYCG 1 cut(s) 370
BstNI CCWGG 1 cut(s) 633
BstNSI RCATGY 1 cut(s) 1391
BstSCI CCNGG 4 cut(s) 313, 585, 631, 824
BstSFI CTRYAG 4 cut(s) 726, 1032, 1473, 1560
BstSLI GKGCMC 1 cut(s) 311
BstUI CGCG 6 cut(s) 284, 320, 353, 372, 1848, 2028
BstV1I GCAGC 7 cut(s) 220, 271, 712, 740, 749, 1528, 1597
BstV2I GAAGAC 2 cut(s) 1248, 1647
BstX2I RGATCY 1 cut(s) 1289
BstXI CCANNNNNNTGG 2 cut(s) 721, 1634
BstYI RGATCY 1 cut(s) 1289
BstZI CGGCCG 1 cut(s) 367
BtgI CCRYGG 4 cut(s) 351, 425, 562, 771
BtsCI GGATG 1 cut(s) 1069
BtsI GCAGTG 1 cut(s) 151
BtsIMutI CAGTG 4 cut(s) 151, 800, 1137, 1407
Cac8I GCNNGC 7 cut(s) 231, 274, 624, 651, 744, 1123, 1514
CfoI GCGC 3 cut(s) 272, 320, 742
Cfr42I CCGCGG 1 cut(s) 354
CseI GACGC 2 cut(s) 214, 448
CviAII CATG 7 cut(s) 426, 443, 563, 772, 892, 1011, 1388
DdeI CTNAG 3 cut(s) 420, 593, 1023
DpnI GATC 5 cut(s) 329, 526, 1291, 1307, 1895
DpnII GATC 5 cut(s) 327, 524, 1289, 1305, 1893
DrdI GACNNNNNNGTC 1 cut(s) 438
DseDI GACNNNNNNGTC 1 cut(s) 438
EaeI YGGCCR 7 cut(s) 285, 357, 367, 664, 711, 924, 1624
EagI CGGCCG 1 cut(s) 367
Eam1104I CTCTTC 7 cut(s) 175, 1751, 1790, 1845, 1931, 1970, 2025
EarI CTCTTC 7 cut(s) 175, 1751, 1790, 1845, 1931, 1970, 2025
EciI GGCGGA 1 cut(s) 107
Ecl136II GAGCTC 1 cut(s) 411
EclXI CGGCCG 1 cut(s) 367
Eco130I CCWWGG 6 cut(s) 425, 562, 771, 1621, 1743, 1923
Eco147I AGGCCT 1 cut(s) 52
Eco24I GRGCYC 2 cut(s) 311, 413
Eco31I GGTCTC 1 cut(s) 1463
Eco47I GGWCC 4 cut(s) 249, 469, 673, 1053
Eco47III AGCGCT 2 cut(s) 271, 741
Eco52I CGGCCG 1 cut(s) 367
Eco53kI GAGCTC 1 cut(s) 411
Eco57I CTGAAG 1 cut(s) 1194
Eco72I CACGTG 1 cut(s) 536
Eco88I CYCGRG 1 cut(s) 1059
EcoICRI GAGCTC 1 cut(s) 411
EcoO109I RGGNCCY 2 cut(s) 249, 307
EcoRII CCWGG 1 cut(s) 631
EcoT14I CCWWGG 6 cut(s) 425, 562, 771, 1621, 1743, 1923
EcoT22I ATGCAT 1 cut(s) 1012
EcoT38I GRGCYC 2 cut(s) 311, 413
ErhI CCWWGG 6 cut(s) 425, 562, 771, 1621, 1743, 1923
Esp3I CGTCTC 2 cut(s) 216, 914
FaeI CATG 7 cut(s) 429, 446, 566, 775, 895, 1014, 1391
FalI AAGNNNNNCTT 4 cut(s) 1376, 1408, 1524, 1556
FaqI GGGAC 3 cut(s) 235, 580, 1066
FatI CATG 7 cut(s) 425, 442, 562, 771, 891, 1010, 1387
FbaI TGATCA 1 cut(s) 1893
FblI GTMKAC 1 cut(s) 489
FokI GGATG 1 cut(s) 1076
FriOI GRGCYC 2 cut(s) 311, 413
FspBI CTAG 4 cut(s) 273, 438, 743, 917
GlaI GCGC 3 cut(s) 271, 319, 741
GsaI CCCAGC 2 cut(s) 256, 1053
GsuI CTGGAG 2 cut(s) 633, 1252
HaeII RGCGCY 2 cut(s) 273, 743
HapII CCGG 6 cut(s) 189, 263, 315, 342, 586, 826
HgaI GACGC 2 cut(s) 214, 448
HhaI GCGC 3 cut(s) 272, 320, 742
Hin1I GRCGYC 3 cut(s) 206, 296, 459
Hin1II CATG 7 cut(s) 429, 446, 566, 775, 895, 1014, 1391
Hin6I GCGC 3 cut(s) 270, 318, 740
HinP1I GCGC 3 cut(s) 270, 318, 740
HincII GTYRAC 2 cut(s) 490, 1318
HindII GTYRAC 2 cut(s) 490, 1318
HinfI GANTC 8 cut(s) 185, 568, 1234, 1244, 1263, 1419, 1480, 1498
HpaI GTTAAC 1 cut(s) 1318
HpaII CCGG 6 cut(s) 189, 263, 315, 342, 586, 826
HphI GGTGA 2 cut(s) 9, 154
Hpy166II GTNNAC 4 cut(s) 490, 642, 1318, 1491
Hpy188I TCNGA 9 cut(s) 384, 582, 596, 1174, 1249, 1342, 1503, 1526, 1651
Hpy188III TCNNGA 9 cut(s) 917, 951, 1061, 1112, 1223, 1238, 1256, 1393, 1588
Hpy8I GTNNAC 4 cut(s) 490, 642, 1318, 1491
Hpy99I CGWCG 5 cut(s) 365, 368, 464, 491, 494
HpyCH4III ACNGT 2 cut(s) 1087, 1495
HpyCH4IV ACGT 2 cut(s) 296, 535
HpyF3I CTNAG 3 cut(s) 420, 593, 1023
HpySE526I ACGT 2 cut(s) 296, 535
Hsp92I GRCGYC 3 cut(s) 206, 296, 459
Hsp92II CATG 7 cut(s) 429, 446, 566, 775, 895, 1014, 1391
HspAI GCGC 3 cut(s) 270, 318, 740
KflI GGGWCCC 1 cut(s) 249
Ksp22I TGATCA 1 cut(s) 1893
KspAI GTTAAC 1 cut(s) 1318
KspI CCGCGG 1 cut(s) 354
Kzo9I GATC 5 cut(s) 327, 524, 1289, 1305, 1893
LmnI GCTCC 5 cut(s) 131, 161, 550, 614, 1014
Lsp1109I GCAGC 7 cut(s) 220, 271, 712, 740, 749, 1528, 1597
LweI GCATC 4 cut(s) 724, 837, 1134, 1346
MaeI CTAG 4 cut(s) 273, 438, 743, 917
MaeII ACGT 2 cut(s) 296, 535
MaeIII GTNAC 4 cut(s) 292, 894, 1036, 1102
MalI GATC 5 cut(s) 329, 526, 1291, 1307, 1895
MboI GATC 5 cut(s) 327, 524, 1289, 1305, 1893
MflI RGATCY 1 cut(s) 1289
MhlI GDGCHC 4 cut(s) 311, 413, 916, 1222
MlsI TGGCCA 1 cut(s) 1626
MluCI AATT 7 cut(s) 750, 816, 928, 966, 1155, 1466, 1572
MluNI TGGCCA 1 cut(s) 1626
MlyI GAGTC 5 cut(s) 179, 562, 1238, 1243, 1489
Mox20I TGGCCA 1 cut(s) 1626
Mph1103I ATGCAT 1 cut(s) 1012
MscI TGGCCA 1 cut(s) 1626
MseI TTAA 2 cut(s) 1089, 1317
MslI CAYNNNNRTG 1 cut(s) 1205
Msp20I TGGCCA 1 cut(s) 1626
MspA1I CMGCKG 1 cut(s) 353
MspI CCGG 6 cut(s) 189, 263, 315, 342, 586, 826
MspR9I CCNGG 4 cut(s) 315, 587, 633, 826
MvaI CCWGG 1 cut(s) 633
MvnI CGCG 6 cut(s) 284, 320, 353, 372, 1848, 2028
NciI CCSGG 3 cut(s) 315, 587, 826
NcoI CCATGG 3 cut(s) 425, 562, 771
NdeII GATC 5 cut(s) 327, 524, 1289, 1305, 1893
NheI GCTAGC 2 cut(s) 272, 742
NlaIII CATG 7 cut(s) 429, 446, 566, 775, 895, 1014, 1391
NlaIV GGNNCC 8 cut(s) 250, 251, 308, 309, 471, 519, 630, 1054
NmeAIII GCCGAG 1 cut(s) 195
NmuCI GTSAC 2 cut(s) 292, 894
NsiI ATGCAT 1 cut(s) 1012
NspI RCATGY 1 cut(s) 1391
PceI AGGCCT 1 cut(s) 52
PcsI WCGNNNNNNNCGW 4 cut(s) 222, 369, 456, 483
PfeI GAWTC 3 cut(s) 1263, 1419, 1498
PflMI CCANNNNNTGG 2 cut(s) 986, 1429
PleI GAGTC 5 cut(s) 179, 562, 1238, 1242, 1488
PmaCI CACGTG 1 cut(s) 536
PmlI CACGTG 1 cut(s) 536
PpsI GAGTC 5 cut(s) 179, 562, 1238, 1242, 1488
Ppu21I YACGTR 1 cut(s) 536
PpuMI RGGWCCY 1 cut(s) 249
Psp124BI GAGCTC 1 cut(s) 413
Psp5II RGGWCCY 1 cut(s) 249
Psp6I CCWGG 1 cut(s) 631
PspCI CACGTG 1 cut(s) 536
PspFI CCCAGC 2 cut(s) 252, 1049
PspGI CCWGG 1 cut(s) 631
PspN4I GGNNCC 8 cut(s) 250, 251, 308, 309, 471, 519, 630, 1054
PspOMI GGGCCC 1 cut(s) 307
PspPPI RGGWCCY 1 cut(s) 249
PstI CTGCAG 2 cut(s) 730, 1477
PsuI RGATCY 1 cut(s) 1289
RseI CAYNNNNRTG 1 cut(s) 1205
SacI GAGCTC 1 cut(s) 413
SacII CCGCGG 1 cut(s) 354
SalI GTCGAC 1 cut(s) 488
SaqAI TTAA 2 cut(s) 1089, 1317
Sau3AI GATC 5 cut(s) 327, 524, 1289, 1305, 1893
SbfI CCTGCAGG 1 cut(s) 1477
SchI GAGTC 5 cut(s) 179, 562, 1238, 1243, 1489
ScrFI CCNGG 4 cut(s) 315, 587, 633, 826
SdaI CCTGCAGG 1 cut(s) 1477
SduI GDGCHC 4 cut(s) 311, 413, 916, 1222
SfaNI GCATC 4 cut(s) 724, 837, 1134, 1346
SfcI CTRYAG 4 cut(s) 726, 1032, 1473, 1560
Sfr303I CCGCGG 1 cut(s) 354
SgrBI CCGCGG 1 cut(s) 354
SgrDI CGTCGACG 1 cut(s) 488
SinI GGWCC 4 cut(s) 249, 469, 673, 1053
SmiMI CAYNNNNRTG 1 cut(s) 1205
SmlI CTYRAG 2 cut(s) 570, 1715
SmoI CTYRAG 2 cut(s) 570, 1715
SpeI ACTAGT 1 cut(s) 437
Sse8387I CCTGCAGG 1 cut(s) 1477
Sse9I AATT 7 cut(s) 750, 816, 928, 966, 1155, 1466, 1572
SseBI AGGCCT 1 cut(s) 52
SspI AATATT 2 cut(s) 1443, 1669
SspMI CTAG 4 cut(s) 273, 438, 743, 917
SstI GAGCTC 1 cut(s) 413
StuI AGGCCT 1 cut(s) 52
StyD4I CCNGG 4 cut(s) 313, 585, 631, 824
StyI CCWWGG 6 cut(s) 425, 562, 771, 1621, 1743, 1923
TaaI ACNGT 2 cut(s) 1087, 1495
TaiI ACGT 2 cut(s) 299, 538
TaqI TCGA 3 cut(s) 450, 489, 1483
TasI AATT 7 cut(s) 750, 816, 928, 966, 1155, 1466, 1572
TfiI GAWTC 3 cut(s) 1263, 1419, 1498
Tru1I TTAA 2 cut(s) 1089, 1317
Tru9I TTAA 2 cut(s) 1089, 1317
TscAI CASTG 4 cut(s) 158, 800, 1144, 1414
TseFI GTSAC 2 cut(s) 292, 894
TseI GCWGC 8 cut(s) 10, 233, 259, 725, 728, 737, 1516, 1610
Tsp45I GTSAC 2 cut(s) 292, 894
TspDTI ATGAA 6 cut(s) 882, 1829, 1901, 2009, 2081, 2087
TspGWI ACGGA 2 cut(s) 368, 772
TspRI CASTG 4 cut(s) 158, 800, 1144, 1414
Van91I CCANNNNNTGG 2 cut(s) 986, 1429
VpaK11BI GGWCC 4 cut(s) 249, 469, 673, 1053
XapI RAATTY 2 cut(s) 1466, 1572
XbaI TCTAGA 1 cut(s) 916
XceI RCATGY 1 cut(s) 1391
XcmI CCANNNNNNNNNTGG 3 cut(s) 442, 1063, 1144
XmiI GTMKAC 1 cut(s) 489
XspI CTAG 4 cut(s) 273, 438, 743, 917
ZraI GACGTC 1 cut(s) 297
Zsp2I ATGCAT 1 cut(s) 1012
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.