MD15G1355000.v1.1
ERF Family

Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin- like GTPase superfamily. TrmE GTPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012258
Physical Location & Seq
Reverse (-)
42736455 .. 42741507
5053 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1355000.v1.1.491

Sequence Viewer

Length: 918 bp
ATGGTACAAACACAGTGCATTGAGCTTCTTACTGAAATTGAAGCCCGTTTGGATTTCGATGATGAGATGCCGCTGCTTGATACAAATGAAATTGTGAATAAAATAAATTCTATGTCGCAAGATGTTGAGAGTGCTCTGGAGACGGCCAATTATGATCAACTTCTGCAATCTGGATTACAGATAGCAATTCTGGGCCGTCCAAACGTTGGGAAGTCAAGTCTACTCAATGCATGGAGCAAAACTGAGAGGGCAATAGTTACAGAAATTGCTGGGACCACTCGAGATGTGGTTGAAGCCAGTATAACGGTTCATGGCATTCCTGTAACCCTTCTTGATACAGCGGGCATTAGGGAAACCAATGACATTGTGGAGAAAATCGGTGTTGAAAGGTCCGAAGCAGTTGCTATGGGTGCTGATGTTATTATAATGGCAATAAGTGCTGTTGATGGCTGGACTCCAGAAGACGCCGAACTTCTAAATAGAATACAATCTAATAAGAAATCTACTGGATCCTCGACCCCAATGATTCTTGTAATTAACAAAATAGACTGCATTCCATCTGATTGCATGGAGGGGATTGAGAAGGATATCAATTCGTTCAGTAAGCATGTTTTGACATCTGCTGTCACTGGTCAAGGAATCCAAGGTTTAGAGACGGCAATATTAGAGATTGTTGGCCTCGACAAAATTTCCGTGGGGAGCCGCAAATGGACTGTCAACCAGAGACAATGCGAGCAGCTTGTTCGGACCAAGGAGGCTTTTGTGAGGTTGAAATCCTCAATCGAAGAGGAAATGCCTTTCGACTTCTGGACAATTGATTTGAGGGACGCGGCAATGGCCCTTGGGCAAATTAGCGGCGACGACATTTCTGAGGAGGTTTTGTCCAACATTTTTGGCAAGTTCTGTATTGGTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MMR_HSR1 MnmE_helical TrmE_N

Protein Analysis

306

Amino Acids

33.35

Weight (kDa)

4.47

Isoelectric Point (pI)

40.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MnmE_helical PF12631 3 - 302 6e-36 MnmE helical domain
MMR_HSR1 PF01926 60 - 181 2e-25 50S ribosome-binding GTPase
FeoB_N PF02421 60 - 220 1.9e-09 Ferrous iron transport protein B
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012258)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78010
fragaria_vesca FvH4_5g38010 FvH4_5g38010
malus_domestica MD08G1177300.v1.1 MD15G1355000.v1.1
prunus_persica Prupe.1G511900_v2.0.a1
pyrus_communis pycom08g15220
rosa_chinensis RchiOBHm_Chr7g0239951
rosa_laevigata RLG00000000715
rosa_multiflora Rmu_sc0000540.1_g000022 Rmu_sc0006825.1_g000001 Rmu_sc0035965.1_g000001
rosa_roxburghii Rroxscaffold_3G00220600
rosa_rugosa Rorug07G0331400
rosa_samantha Rh7AG485100 Rh7BG458100 Rh7CG502400 Rh7DG470700
rosa_wichuraiana Rw7G040690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 425
AccB7I CCANNNNNTGG 1 cut(s) 206
AccI GTMKAC 1 cut(s) 220
AccII CGCG 1 cut(s) 830
AciI CCGC 5 cut(s) 71, 341, 703, 830, 855
AclI AACGTT 1 cut(s) 204
AclWI GGATC 2 cut(s) 504, 517
AcoI YGGCCR 1 cut(s) 144
AcsI RAATTY 2 cut(s) 106, 687
AcyI GRCGYC 1 cut(s) 465
AfaI GTAC 1 cut(s) 6
AfiI CCNNNNNNNGG 1 cut(s) 206
AgsI TTSAA 4 cut(s) 41, 293, 386, 772
AluBI AGCT 2 cut(s) 25, 739
AluI AGCT 2 cut(s) 25, 739
Alw21I GWGCWC 1 cut(s) 136
Alw26I GTCTC 3 cut(s) 134, 647, 718
AlwI GGATC 2 cut(s) 504, 517
Ama87I CYCGRG 1 cut(s) 279
AoxI GGCC 4 cut(s) 144, 193, 676, 837
ApeKI GCWGC 2 cut(s) 73, 736
ApoI RAATTY 2 cut(s) 106, 687
ArsI GACNNNNNNTTYG 4 cut(s) 699, 731, 802, 834
AspS9I GGNCC 5 cut(s) 193, 273, 390, 747, 838
AvaI CYCGRG 1 cut(s) 279
AvaII GGWCC 3 cut(s) 273, 390, 747
BamHI GGATCC 1 cut(s) 509
BbsI GAAGAC 1 cut(s) 468
Bbv12I GWGCWC 1 cut(s) 136
BbvI GCAGC 2 cut(s) 60, 748
BccI CCATC 2 cut(s) 440, 565
BceAI ACGGC 3 cut(s) 159, 180, 672
BcgI CGANNNNNNTGC 4 cut(s) 383, 417, 725, 759
BclI TGATCA 1 cut(s) 154
BcoDI GTCTC 3 cut(s) 134, 647, 718
BisI GCNGC 6 cut(s) 71, 74, 703, 737, 831, 856
BlsI GCNGC 6 cut(s) 72, 75, 704, 738, 832, 857
Bme18I GGWCC 3 cut(s) 273, 390, 747
BmeT110I CYCGRG 1 cut(s) 279
BmgT120I GGNCC 5 cut(s) 193, 273, 390, 747, 838
BmiI GGNNCC 3 cut(s) 274, 511, 701
BmsI GCATC 1 cut(s) 57
BpiI GAAGAC 1 cut(s) 468
BpmI CTGGAG 2 cut(s) 158, 441
BsaHI GRCGYC 1 cut(s) 465
BsaJI CCNNGG 4 cut(s) 643, 693, 750, 841
BsaXI ACNNNNNCTCC 2 cut(s) 866, 896
Bsc4I CCNNNNNNNGG 1 cut(s) 206
Bse1I ACTGG 3 cut(s) 297, 511, 634
Bse3DI GCAATG 1 cut(s) 840
BseDI CCNNGG 4 cut(s) 643, 693, 750, 841
BseLI CCNNNNNNNGG 1 cut(s) 206
BseMI GCAATG 1 cut(s) 840
BseMII CTCAG 2 cut(s) 234, 861
BseNI ACTGG 3 cut(s) 297, 511, 634
BseRI GAGGAG 1 cut(s) 887
BseXI GCAGC 2 cut(s) 60, 748
BseYI CCCAGC 1 cut(s) 269
Bsh1236I CGCG 1 cut(s) 830
BshFI GGCC 4 cut(s) 146, 195, 678, 839
BsiHKAI GWGCWC 1 cut(s) 136
BsiHKCI CYCGRG 1 cut(s) 279
BslFI GGGAC 2 cut(s) 286, 839
BslI CCNNNNNNNGG 1 cut(s) 206
BsmAI GTCTC 3 cut(s) 134, 647, 718
BsmBI CGTCTC 2 cut(s) 134, 647
BsmFI GGGAC 2 cut(s) 286, 839
BsmI GAATGC 2 cut(s) 315, 552
BsnI GGCC 4 cut(s) 146, 195, 678, 839
BsoBI CYCGRG 1 cut(s) 279
Bsp1286I GDGCHC 1 cut(s) 136
Bsp143I GATC 2 cut(s) 154, 509
BspACI CCGC 5 cut(s) 71, 341, 703, 830, 855
BspANI GGCC 4 cut(s) 146, 195, 678, 839
BspCNI CTCAG 2 cut(s) 235, 862
BspFNI CGCG 1 cut(s) 830
BspLI GGNNCC 3 cut(s) 274, 511, 701
BspPI GGATC 2 cut(s) 504, 517
BsrDI GCAATG 1 cut(s) 840
BsrI ACTGG 3 cut(s) 297, 511, 634
BssECI CCNNGG 4 cut(s) 643, 693, 750, 841
BssMI GATC 2 cut(s) 154, 509
BssNI GRCGYC 1 cut(s) 465
BssT1I CCWWGG 3 cut(s) 643, 750, 841
Bst4CI ACNGT 3 cut(s) 15, 307, 715
Bst6I CTCTTC 1 cut(s) 780
BstACI GRCGYC 1 cut(s) 465
BstAPI GCANNNNNTGC 1 cut(s) 437
BstC8I GCNNGC 2 cut(s) 343, 734
BstDEI CTNAG 2 cut(s) 243, 870
BstDSI CCRYGG 1 cut(s) 693
BstFNI CGCG 1 cut(s) 830
BstKTI GATC 2 cut(s) 157, 512
BstMAI GTCTC 3 cut(s) 134, 647, 718
BstMBI GATC 2 cut(s) 154, 509
BstMWI GCNNNNNNNGC 3 cut(s) 410, 437, 836
BstNSI RCATGY 1 cut(s) 611
BstUI CGCG 1 cut(s) 830
BstV1I GCAGC 2 cut(s) 60, 748
BstV2I GAAGAC 1 cut(s) 468
BstX2I RGATCY 1 cut(s) 509
BstYI RGATCY 1 cut(s) 509
BsuRI GGCC 4 cut(s) 146, 195, 678, 839
BtgI CCRYGG 1 cut(s) 693
BtsIMutI CAGTG 2 cut(s) 20, 627
Cac8I GCNNGC 2 cut(s) 343, 734
Cfr13I GGNCC 5 cut(s) 193, 273, 390, 747, 838
CseI GACGC 2 cut(s) 473, 836
Csp6I GTAC 1 cut(s) 5
CviAII CATG 4 cut(s) 231, 311, 568, 608
CviQI GTAC 1 cut(s) 5
DdeI CTNAG 2 cut(s) 243, 870
DpnI GATC 2 cut(s) 156, 511
DpnII GATC 2 cut(s) 154, 509
EaeI YGGCCR 1 cut(s) 144
Eam1104I CTCTTC 1 cut(s) 780
EarI CTCTTC 1 cut(s) 780
Eco130I CCWWGG 3 cut(s) 643, 750, 841
Eco32I GATATC 1 cut(s) 589
Eco47I GGWCC 3 cut(s) 273, 390, 747
Eco88I CYCGRG 1 cut(s) 279
EcoRV GATATC 1 cut(s) 589
EcoT14I CCWWGG 3 cut(s) 643, 750, 841
EcoT22I ATGCAT 1 cut(s) 232
ErhI CCWWGG 3 cut(s) 643, 750, 841
Esp3I CGTCTC 2 cut(s) 134, 647
FaeI CATG 4 cut(s) 234, 314, 571, 611
FaiI YATR 9 cut(s) 113, 153, 232, 302, 312, 407, 425, 569, 609
FaqI GGGAC 2 cut(s) 286, 839
FatI CATG 4 cut(s) 230, 310, 567, 607
FauI CCCGC 1 cut(s) 334
FbaI TGATCA 1 cut(s) 154
FblI GTMKAC 1 cut(s) 220
Fnu4HI GCNGC 6 cut(s) 71, 74, 703, 737, 831, 856
Fsp4HI GCNGC 6 cut(s) 71, 74, 703, 737, 831, 856
GluI GCNGC 6 cut(s) 71, 74, 703, 737, 831, 856
GsaI CCCAGC 1 cut(s) 273
GsuI CTGGAG 2 cut(s) 158, 441
HaeIII GGCC 4 cut(s) 146, 195, 678, 839
HgaI GACGC 2 cut(s) 473, 836
Hin1I GRCGYC 1 cut(s) 465
Hin1II CATG 4 cut(s) 234, 314, 571, 611
HincII GTYRAC 1 cut(s) 718
HindII GTYRAC 1 cut(s) 718
HinfI GANTC 3 cut(s) 454, 526, 639
Hpy166II GTNNAC 2 cut(s) 221, 718
Hpy188I TCNGA 4 cut(s) 394, 562, 747, 871
Hpy188III TCNNGA 6 cut(s) 137, 171, 281, 332, 458, 808
Hpy8I GTNNAC 2 cut(s) 221, 718
Hpy99I CGWCG 1 cut(s) 863
HpyAV CCTTC 2 cut(s) 338, 577
HpyCH4III ACNGT 3 cut(s) 15, 307, 715
HpyCH4IV ACGT 1 cut(s) 204
HpyCH4V TGCA 5 cut(s) 18, 166, 230, 552, 567
HpyF10VI GCNNNNNNNGC 3 cut(s) 410, 437, 836
HpyF3I CTNAG 2 cut(s) 243, 870
HpySE526I ACGT 1 cut(s) 204
Hsp92I GRCGYC 1 cut(s) 465
Hsp92II CATG 4 cut(s) 234, 314, 571, 611
Ksp22I TGATCA 1 cut(s) 154
Kzo9I GATC 2 cut(s) 154, 509
LmnI GCTCC 2 cut(s) 234, 699
Lsp1109I GCAGC 2 cut(s) 60, 748
LweI GCATC 1 cut(s) 57
MaeII ACGT 1 cut(s) 204
MaeIII GTNAC 3 cut(s) 256, 322, 625
MalI GATC 2 cut(s) 156, 511
MboI GATC 2 cut(s) 154, 509
MboII GAAGA 2 cut(s) 473, 797
MfeI CAATTG 1 cut(s) 813
MflI RGATCY 1 cut(s) 509
MhlI GDGCHC 1 cut(s) 136
MlyI GAGTC 1 cut(s) 448
MmeI TCCRAC 1 cut(s) 909
Mph1103I ATGCAT 1 cut(s) 232
MseI TTAA 1 cut(s) 537
MspA1I CMGCKG 2 cut(s) 73, 341
MunI CAATTG 1 cut(s) 813
Mva1269I GAATGC 2 cut(s) 315, 552
MvnI CGCG 1 cut(s) 830
MwoI GCNNNNNNNGC 3 cut(s) 410, 437, 836
NdeII GATC 2 cut(s) 154, 509
NlaIII CATG 4 cut(s) 234, 314, 571, 611
NlaIV GGNNCC 3 cut(s) 274, 511, 701
NmuCI GTSAC 1 cut(s) 625
NsiI ATGCAT 1 cut(s) 232
NspI RCATGY 1 cut(s) 611
PaeR7I CTCGAG 1 cut(s) 279
PctI GAATGC 2 cut(s) 315, 552
PfeI GAWTC 2 cut(s) 526, 639
PflMI CCANNNNNTGG 1 cut(s) 206
PkrI GCNGC 6 cut(s) 72, 75, 704, 738, 832, 857
PleI GAGTC 1 cut(s) 448
PpsI GAGTC 1 cut(s) 448
PsiI TTATAA 1 cut(s) 425
Psp1406I AACGTT 1 cut(s) 204
PspFI CCCAGC 1 cut(s) 269
PspN4I GGNNCC 3 cut(s) 274, 511, 701
PspPI GGNCC 5 cut(s) 193, 273, 390, 747, 838
PsuI RGATCY 1 cut(s) 509
RsaI GTAC 1 cut(s) 6
RsaNI GTAC 1 cut(s) 5
SaqAI TTAA 1 cut(s) 537
SatI GCNGC 6 cut(s) 71, 74, 703, 737, 831, 856
Sau3AI GATC 2 cut(s) 154, 509
Sau96I GGNCC 5 cut(s) 193, 273, 390, 747, 838
SchI GAGTC 1 cut(s) 448
SduI GDGCHC 1 cut(s) 136
SetI ASST 7 cut(s) 27, 207, 392, 649, 741, 770, 879
SfaNI GCATC 1 cut(s) 57
Sfr274I CTCGAG 1 cut(s) 279
SinI GGWCC 3 cut(s) 273, 390, 747
SlaI CTCGAG 1 cut(s) 279
SmlI CTYRAG 1 cut(s) 279
SmoI CTYRAG 1 cut(s) 279
SsiI CCGC 5 cut(s) 71, 341, 703, 830, 855
SspI AATATT 1 cut(s) 663
StyI CCWWGG 3 cut(s) 643, 750, 841
TaaI ACNGT 3 cut(s) 15, 307, 715
TaiI ACGT 1 cut(s) 207
TaqI TCGA 6 cut(s) 57, 280, 515, 681, 783, 801
TauI GCSGC 4 cut(s) 73, 705, 833, 858
TfiI GAWTC 2 cut(s) 526, 639
Tru1I TTAA 1 cut(s) 537
Tru9I TTAA 1 cut(s) 537
TscAI CASTG 2 cut(s) 20, 634
TseFI GTSAC 1 cut(s) 625
TseI GCWGC 2 cut(s) 73, 736
Tsp45I GTSAC 1 cut(s) 625
TspDTI ATGAA 2 cut(s) 102, 299
TspGWI ACGGA 1 cut(s) 682
TspRI CASTG 2 cut(s) 20, 634
Van91I CCANNNNNTGG 1 cut(s) 206
VpaK11BI GGWCC 3 cut(s) 273, 390, 747
XapI RAATTY 2 cut(s) 106, 687
XceI RCATGY 1 cut(s) 611
XcmI CCANNNNNNNNNTGG 2 cut(s) 283, 364
XhoI CTCGAG 1 cut(s) 279
XmiI GTMKAC 1 cut(s) 220
Zsp2I ATGCAT 1 cut(s) 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.