Rw7G040690
ERF Family

Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin- like GTPase superfamily. TrmE GTPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0012258
Physical Location & Seq
Reverse (-)
63411927 .. 63415954
4028 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G040690.1

Sequence Viewer

Length: 1698 bp
ATGGCCTCTCTGCTCTCACCTCTCCGCTTTCGCCATTTCCTTTACACAAGGCCTAAACTAAAACTGAAACCCCCTTATCTAATCTCTGCCGCCTCTTTCAAACCCCTCCTCAAACTCCGCCCTCGCTCCCCTCCCTATTTACACTCCTGCCACTCCTCCTTCACCGCCTCTTCCCCACTCGCAGACTCCGACTACAAAACCCAGACGCCGCTTCTCGGCAGAGACGAGCGAGCTGCCGTAGTGAGTGGGTCCCAGCCGCAGCCGGAGAGCGCGAGCACAATCGCGGCCATTGTGACGTCAGTTGGGGGCCCACCCGGCGCGGTTGGGATCGTGCGGCTGTCCGGCCCGTCCGCGGTGGCCGTCGTCGGCCGCGTGTTCCGTCCGAGCAAGAAAAAGAGAAAGAGCAAGAGCTCTGGTGCTGAGCCATGGCGACCCACTAGTCATGTAATCGAATACGGCGTCGTTTTGGACCCCAACGGCAATGTCGTCGACGAGGTTTTAGCTGTGCCAATGTTGGCCCCAAGATCATATACACGTGAAGATGTGGTGGAGCTTCAATGCCATGGGACTCAAGTGTGTCTGAACCGGGTTCTAAGAGCTTGTTTAGAAGCTGGAGCAAGGCTTGCGGAACCAGGGGAGTTCACTCTGCGTGCCTTTCTCAATGGCCGCTTGGACCTGACCCAAGCTGAAAATGTTGATAAACTCATTTCGGCCAAGTCTGTGGCTGCAGCAGATGCAGCACTAGCTGCAATTCAGGGTGGCTTTTCTTCCATGGTAAAATCTGTAAGATTTCAGTGCATTGAGTTGCTTACTGAAATTGAAGCCCGGTTAGATTTTGATGATGAGATGCCGCCGCTTGATACCAATGAAGTTGTGAATAAAATACATTCCATGTCACAAGATGTTGAGAGTGCTCTAGAGACGGCCAATTATGACCAACTTCTGCAATCTGGATTACAGATAGCAATTCTGGGCCGTCCAAATGTTGGGAAATCAAGCCTCCTCAATGCATGGAGCAAAACTGAGAGAGCTATAGTTACAGAAGTTGCTGGGACCACTCGGGATGTGGTTGAAGCCAGTATTACAGTTAATGGTATTCCTGTAACCCTTCTTGATACAGCAGGCATCAGGGAAACCACTGACATAGTGGAGAAAATTGGTGTGGAGAGGTCTGAAGCAGTTGCTATGGGTGCTGATGTCATTATTATGGCAATAAGTGCTCTTGATGGCTGGAATCCAGAAGACTCTGAACTTCTTGACAGAATCCAATCTAACAAGAAATCAACTGGATCTTCAACCCCAATGATCCTTGTAGTTAACAAAATCGATTGTGTTGCATCTGATTGCTTGGAGTGGGTTCAAAAAAATGTTGGTTCTTTCAATAAGCATGTCTTGACATCTGCCATCACTGGTCAAGGAATCGAAGATTTGGAGAAGGCAATATTAGATAGTGTTGGTCTCAACAAATTTCCTGCAGGGAGTCGAAGGTGGACTGTGAACCAGAGACAATGCGAGCAGCTTGTCCGAACCAAAGAGGCTCTTGCAAGGTTGAAATCCTCTATAGAGGAGGAAATTCCTTTTGACTTCTGGACGATTGATTTGAGGGAAGCTGCAATGGCCCTTGGCCAAATAAGTGGAGAAGACATTTCAGAGGAGGTTTTGACCAATATTTTTGGCAAGTTCTGCATTGGTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MMR_HSR1 MnmE_helical TrmE_N

Protein Analysis

565

Amino Acids

61.19

Weight (kDa)

5.84

Isoelectric Point (pI)

44.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TrmE_N PF10396 93 - 223 3.4e-37 GTP-binding protein TrmE N-terminus
MnmE_helical PF12631 226 - 562 4.2e-49 MnmE helical domain
MMR_HSR1 PF01926 320 - 441 1.3e-24 50S ribosome-binding GTPase
FeoB_N PF02421 320 - 481 4.4e-09 Ferrous iron transport protein B
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012258)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78010
fragaria_vesca FvH4_5g38010 FvH4_5g38010
malus_domestica MD08G1177300.v1.1 MD15G1355000.v1.1
prunus_persica Prupe.1G511900_v2.0.a1
pyrus_communis pycom08g15220
rosa_chinensis RchiOBHm_Chr7g0239951
rosa_laevigata RLG00000000715
rosa_multiflora Rmu_sc0000540.1_g000022 Rmu_sc0006825.1_g000001 Rmu_sc0035965.1_g000001
rosa_roxburghii Rroxscaffold_3G00220600
rosa_rugosa Rorug07G0331400
rosa_samantha Rh7AG485100 Rh7BG458100 Rh7CG502400 Rh7DG470700
rosa_wichuraiana Rw7G040690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 438
AatII GACGTC 1 cut(s) 299
AccB7I CCANNNNNTGG 1 cut(s) 986
AccI GTMKAC 1 cut(s) 489
AccII CGCG 5 cut(s) 272, 284, 320, 353, 372
AclWI GGATC 3 cut(s) 335, 1297, 1300
AcoI YGGCCR 7 cut(s) 285, 357, 367, 664, 711, 924, 1624
AcsI RAATTY 2 cut(s) 1466, 1572
AcuI CTGAAG 1 cut(s) 1194
AcvI CACGTG 1 cut(s) 536
AcyI GRCGYC 3 cut(s) 206, 296, 459
AfiI CCNNNNNNNGG 3 cut(s) 215, 352, 986
AflIII ACRYGT 1 cut(s) 533
AgsI TTSAA 8 cut(s) 100, 557, 821, 1073, 1296, 1361, 1381, 1552
AhlI ACTAGT 1 cut(s) 437
AjnI CCWGG 1 cut(s) 631
Alw21I GWGCWC 4 cut(s) 278, 413, 916, 1222
Alw26I GTCTC 4 cut(s) 216, 914, 1463, 1498
AlwI GGATC 3 cut(s) 335, 1297, 1300
Ama87I CYCGRG 1 cut(s) 1059
ApaI GGGCCC 1 cut(s) 311
ApeKI GCWGC 8 cut(s) 233, 259, 725, 728, 737, 746, 1516, 1610
ApoI RAATTY 2 cut(s) 1466, 1572
ArsI GACNNNNNNTTYG 4 cut(s) 444, 476, 1582, 1614
AspLEI GCGC 2 cut(s) 272, 320
AsuC2I CCSGG 3 cut(s) 315, 587, 826
AsuHPI GGTGA 2 cut(s) 9, 154
AvaI CYCGRG 1 cut(s) 1059
AvaII GGWCC 4 cut(s) 249, 469, 673, 1053
BaeGI GKGCMC 1 cut(s) 311
BalI TGGCCA 1 cut(s) 1626
BanII GRGCYC 2 cut(s) 311, 413
BbrPI CACGTG 1 cut(s) 536
BbsI GAAGAC 2 cut(s) 1248, 1647
Bbv12I GWGCWC 4 cut(s) 278, 413, 916, 1222
BbvI GCAGC 8 cut(s) 220, 271, 712, 733, 740, 749, 1528, 1597
BccI CCATC 2 cut(s) 1220, 1412
BceAI ACGGC 6 cut(s) 221, 344, 472, 493, 939, 960
BcgI CGANNNNNNTGC 6 cut(s) 215, 249, 469, 503, 1316, 1350
BciT130I CCWGG 1 cut(s) 633
BcnI CCSGG 3 cut(s) 315, 587, 826
BcoDI GTCTC 4 cut(s) 216, 914, 1463, 1498
BcuI ACTAGT 1 cut(s) 437
BfaI CTAG 3 cut(s) 438, 743, 917
BfmI CTRYAG 4 cut(s) 726, 1032, 1473, 1560
BglI GCCNNNNNGGC 1 cut(s) 315
BlpI GCTNAGC 1 cut(s) 420
Bme1390I CCNGG 4 cut(s) 315, 587, 633, 826
Bme18I GGWCC 4 cut(s) 249, 469, 673, 1053
BmeT110I CYCGRG 1 cut(s) 1059
BmiI GGNNCC 8 cut(s) 250, 251, 308, 309, 471, 519, 630, 1054
BmrFI CCNGG 4 cut(s) 315, 587, 633, 826
BmsI GCATC 4 cut(s) 724, 837, 1134, 1346
BpiI GAAGAC 2 cut(s) 1248, 1647
BpmI CTGGAG 1 cut(s) 633
Bpu1102I GCTNAGC 1 cut(s) 420
BpuEI CTTGAG 1 cut(s) 555
BpuMI CCSGG 3 cut(s) 315, 587, 826
Bsa29I ATCGAT 1 cut(s) 1326
BsaAI YACGTR 1 cut(s) 536
BsaHI GRCGYC 3 cut(s) 206, 296, 459
BsaI GGTCTC 1 cut(s) 1463
BsaJI CCNNGG 6 cut(s) 351, 425, 562, 632, 771, 1621
Bsc4I CCNNNNNNNGG 3 cut(s) 215, 352, 986
Bse1I ACTGG 3 cut(s) 1077, 1291, 1414
Bse3DI GCAATG 2 cut(s) 487, 1620
BseBI CCWGG 1 cut(s) 633
BseCI ATCGAT 1 cut(s) 1326
BseDI CCNNGG 6 cut(s) 351, 425, 562, 632, 771, 1621
BseGI GGATG 1 cut(s) 1069
BseLI CCNNNNNNNGG 3 cut(s) 215, 352, 986
BseMI GCAATG 2 cut(s) 487, 1620
BseMII CTCAG 2 cut(s) 411, 1014
BseNI ACTGG 3 cut(s) 1077, 1291, 1414
BseRI GAGGAG 5 cut(s) 98, 145, 992, 1580, 1667
BseSI GKGCMC 1 cut(s) 311
BseX3I CGGCCG 1 cut(s) 367
BseXI GCAGC 8 cut(s) 220, 271, 712, 733, 740, 749, 1528, 1597
BseYI CCCAGC 2 cut(s) 252, 1049
Bsh1236I CGCG 5 cut(s) 272, 284, 320, 353, 372
Bsh1285I CGRYCG 1 cut(s) 370
BshVI ATCGAT 1 cut(s) 1326
BsiEI CGRYCG 1 cut(s) 370
BsiHKAI GWGCWC 4 cut(s) 278, 413, 916, 1222
BsiHKCI CYCGRG 1 cut(s) 1059
BsiSI CCGG 5 cut(s) 263, 315, 342, 586, 826
BslFI GGGAC 3 cut(s) 235, 580, 1066
BslI CCNNNNNNNGG 3 cut(s) 215, 352, 986
BsmAI GTCTC 4 cut(s) 216, 914, 1463, 1498
BsmBI CGTCTC 2 cut(s) 216, 914
BsmFI GGGAC 3 cut(s) 235, 580, 1066
Bso31I GGTCTC 1 cut(s) 1463
BsoBI CYCGRG 1 cut(s) 1059
Bsp120I GGGCCC 1 cut(s) 307
Bsp1286I GDGCHC 5 cut(s) 278, 311, 413, 916, 1222
Bsp143I GATC 4 cut(s) 327, 524, 1289, 1305
Bsp1720I GCTNAGC 1 cut(s) 420
Bsp19I CCATGG 3 cut(s) 425, 562, 771
BspCNI CTCAG 2 cut(s) 412, 1015
BspDI ATCGAT 1 cut(s) 1326
BspFNI CGCG 5 cut(s) 272, 284, 320, 353, 372
BspLI GGNNCC 8 cut(s) 250, 251, 308, 309, 471, 519, 630, 1054
BspMAI CTGCAG 2 cut(s) 730, 1477
BspPI GGATC 3 cut(s) 335, 1297, 1300
BspTNI GGTCTC 1 cut(s) 1463
BsrDI GCAATG 2 cut(s) 487, 1620
BsrI ACTGG 3 cut(s) 1077, 1291, 1414
BssECI CCNNGG 6 cut(s) 351, 425, 562, 632, 771, 1621
BssMI GATC 4 cut(s) 327, 524, 1289, 1305
BssNI GRCGYC 3 cut(s) 206, 296, 459
BssT1I CCWWGG 4 cut(s) 425, 562, 771, 1621
Bst2UI CCWGG 1 cut(s) 633
Bst4CI ACNGT 2 cut(s) 1087, 1495
Bst6I CTCTTC 1 cut(s) 175
BstACI GRCGYC 3 cut(s) 206, 296, 459
BstAPI GCANNNNNTGC 5 cut(s) 623, 734, 746, 1217, 1683
BstBAI YACGTR 1 cut(s) 536
BstC8I GCNNGC 6 cut(s) 231, 274, 624, 651, 1123, 1514
BstDEI CTNAG 3 cut(s) 420, 593, 1023
BstDSI CCRYGG 4 cut(s) 351, 425, 562, 771
BstF5I GGATG 1 cut(s) 1069
BstFNI CGCG 5 cut(s) 272, 284, 320, 353, 372
BstHHI GCGC 2 cut(s) 272, 320
BstKTI GATC 4 cut(s) 330, 527, 1292, 1308
BstMAI GTCTC 4 cut(s) 216, 914, 1463, 1498
BstMBI GATC 4 cut(s) 327, 524, 1289, 1305
BstMCI CGRYCG 1 cut(s) 370
BstNI CCWGG 1 cut(s) 633
BstNSI RCATGY 1 cut(s) 1391
BstSCI CCNGG 4 cut(s) 313, 585, 631, 824
BstSFI CTRYAG 4 cut(s) 726, 1032, 1473, 1560
BstSLI GKGCMC 1 cut(s) 311
BstUI CGCG 5 cut(s) 272, 284, 320, 353, 372
BstV1I GCAGC 8 cut(s) 220, 271, 712, 733, 740, 749, 1528, 1597
BstV2I GAAGAC 2 cut(s) 1248, 1647
BstX2I RGATCY 1 cut(s) 1289
BstXI CCANNNNNNTGG 2 cut(s) 721, 1634
BstYI RGATCY 1 cut(s) 1289
BstZI CGGCCG 1 cut(s) 367
Bsu15I ATCGAT 1 cut(s) 1326
BsuTUI ATCGAT 1 cut(s) 1326
BtgI CCRYGG 4 cut(s) 351, 425, 562, 771
BtsCI GGATG 1 cut(s) 1069
BtsIMutI CAGTG 3 cut(s) 800, 1137, 1407
Cac8I GCNNGC 6 cut(s) 231, 274, 624, 651, 1123, 1514
CfoI GCGC 2 cut(s) 272, 320
Cfr42I CCGCGG 1 cut(s) 354
ClaI ATCGAT 1 cut(s) 1326
CseI GACGC 2 cut(s) 214, 448
CviAII CATG 7 cut(s) 426, 443, 563, 772, 892, 1011, 1388
DdeI CTNAG 3 cut(s) 420, 593, 1023
DpnI GATC 4 cut(s) 329, 526, 1291, 1307
DpnII GATC 4 cut(s) 327, 524, 1289, 1305
DrdI GACNNNNNNGTC 1 cut(s) 438
DseDI GACNNNNNNGTC 1 cut(s) 438
EaeI YGGCCR 7 cut(s) 285, 357, 367, 664, 711, 924, 1624
EagI CGGCCG 1 cut(s) 367
Eam1104I CTCTTC 1 cut(s) 175
EarI CTCTTC 1 cut(s) 175
EciI GGCGGA 1 cut(s) 107
Ecl136II GAGCTC 1 cut(s) 411
EclXI CGGCCG 1 cut(s) 367
Eco130I CCWWGG 4 cut(s) 425, 562, 771, 1621
Eco147I AGGCCT 1 cut(s) 52
Eco24I GRGCYC 2 cut(s) 311, 413
Eco31I GGTCTC 1 cut(s) 1463
Eco47I GGWCC 4 cut(s) 249, 469, 673, 1053
Eco52I CGGCCG 1 cut(s) 367
Eco53kI GAGCTC 1 cut(s) 411
Eco57I CTGAAG 1 cut(s) 1194
Eco72I CACGTG 1 cut(s) 536
Eco88I CYCGRG 1 cut(s) 1059
EcoICRI GAGCTC 1 cut(s) 411
EcoO109I RGGNCCY 2 cut(s) 249, 307
EcoRII CCWGG 1 cut(s) 631
EcoT14I CCWWGG 4 cut(s) 425, 562, 771, 1621
EcoT22I ATGCAT 1 cut(s) 1012
EcoT38I GRGCYC 2 cut(s) 311, 413
ErhI CCWWGG 4 cut(s) 425, 562, 771, 1621
Esp3I CGTCTC 2 cut(s) 216, 914
FaeI CATG 7 cut(s) 429, 446, 566, 775, 895, 1014, 1391
FalI AAGNNNNNCTT 4 cut(s) 1376, 1408, 1524, 1556
FaqI GGGAC 3 cut(s) 235, 580, 1066
FatI CATG 7 cut(s) 425, 442, 562, 771, 891, 1010, 1387
FblI GTMKAC 1 cut(s) 489
FokI GGATG 1 cut(s) 1076
FriOI GRGCYC 2 cut(s) 311, 413
FspBI CTAG 3 cut(s) 438, 743, 917
GlaI GCGC 2 cut(s) 271, 319
GsaI CCCAGC 2 cut(s) 256, 1053
GsuI CTGGAG 1 cut(s) 633
HapII CCGG 5 cut(s) 263, 315, 342, 586, 826
HgaI GACGC 2 cut(s) 214, 448
HhaI GCGC 2 cut(s) 272, 320
Hin1I GRCGYC 3 cut(s) 206, 296, 459
Hin1II CATG 7 cut(s) 429, 446, 566, 775, 895, 1014, 1391
Hin6I GCGC 2 cut(s) 270, 318
HinP1I GCGC 2 cut(s) 270, 318
HincII GTYRAC 2 cut(s) 490, 1318
HindII GTYRAC 2 cut(s) 490, 1318
HinfI GANTC 7 cut(s) 185, 568, 1234, 1244, 1263, 1419, 1480
HpaI GTTAAC 1 cut(s) 1318
HpaII CCGG 5 cut(s) 263, 315, 342, 586, 826
HphI GGTGA 2 cut(s) 9, 154
Hpy166II GTNNAC 5 cut(s) 490, 642, 1318, 1491, 1498
Hpy188I TCNGA 8 cut(s) 190, 384, 582, 1174, 1249, 1342, 1526, 1651
Hpy188III TCNNGA 9 cut(s) 917, 951, 1061, 1112, 1223, 1238, 1256, 1393, 1588
Hpy8I GTNNAC 5 cut(s) 490, 642, 1318, 1491, 1498
Hpy99I CGWCG 5 cut(s) 365, 368, 464, 491, 494
HpyAV CCTTC 4 cut(s) 169, 1118, 1429, 1479
HpyCH4III ACNGT 2 cut(s) 1087, 1495
HpyCH4IV ACGT 2 cut(s) 296, 535
HpyF3I CTNAG 3 cut(s) 420, 593, 1023
HpySE526I ACGT 2 cut(s) 296, 535
Hsp92I GRCGYC 3 cut(s) 206, 296, 459
Hsp92II CATG 7 cut(s) 429, 446, 566, 775, 895, 1014, 1391
HspAI GCGC 2 cut(s) 270, 318
KflI GGGWCCC 1 cut(s) 249
KspAI GTTAAC 1 cut(s) 1318
KspI CCGCGG 1 cut(s) 354
Kzo9I GATC 4 cut(s) 327, 524, 1289, 1305
LmnI GCTCC 4 cut(s) 131, 550, 614, 1014
Lsp1109I GCAGC 8 cut(s) 220, 271, 712, 733, 740, 749, 1528, 1597
LweI GCATC 4 cut(s) 724, 837, 1134, 1346
MaeI CTAG 3 cut(s) 438, 743, 917
MaeII ACGT 2 cut(s) 296, 535
MaeIII GTNAC 4 cut(s) 292, 894, 1036, 1102
MalI GATC 4 cut(s) 329, 526, 1291, 1307
MboI GATC 4 cut(s) 327, 524, 1289, 1305
MboII GAAGA 7 cut(s) 162, 551, 759, 1253, 1284, 1436, 1652
MflI RGATCY 1 cut(s) 1289
MhlI GDGCHC 5 cut(s) 278, 311, 413, 916, 1222
MlsI TGGCCA 1 cut(s) 1626
MluCI AATT 7 cut(s) 750, 816, 928, 966, 1155, 1466, 1572
MluNI TGGCCA 1 cut(s) 1626
MlyI GAGTC 4 cut(s) 179, 562, 1238, 1489
MmeI TCCRAC 1 cut(s) 213
Mox20I TGGCCA 1 cut(s) 1626
Mph1103I ATGCAT 1 cut(s) 1012
MscI TGGCCA 1 cut(s) 1626
MseI TTAA 2 cut(s) 1089, 1317
MslI CAYNNNNRTG 1 cut(s) 1205
Msp20I TGGCCA 1 cut(s) 1626
MspA1I CMGCKG 1 cut(s) 353
MspI CCGG 5 cut(s) 263, 315, 342, 586, 826
MspR9I CCNGG 4 cut(s) 315, 587, 633, 826
MvaI CCWGG 1 cut(s) 633
MvnI CGCG 5 cut(s) 272, 284, 320, 353, 372
NciI CCSGG 3 cut(s) 315, 587, 826
NcoI CCATGG 3 cut(s) 425, 562, 771
NdeII GATC 4 cut(s) 327, 524, 1289, 1305
NlaIII CATG 7 cut(s) 429, 446, 566, 775, 895, 1014, 1391
NlaIV GGNNCC 8 cut(s) 250, 251, 308, 309, 471, 519, 630, 1054
NmeAIII GCCGAG 1 cut(s) 195
NmuCI GTSAC 2 cut(s) 292, 894
NsiI ATGCAT 1 cut(s) 1012
NspI RCATGY 1 cut(s) 1391
PceI AGGCCT 1 cut(s) 52
PcsI WCGNNNNNNNCGW 5 cut(s) 186, 222, 369, 456, 483
PfeI GAWTC 3 cut(s) 1234, 1263, 1419
PflMI CCANNNNNTGG 1 cut(s) 986
PleI GAGTC 4 cut(s) 179, 562, 1238, 1488
PmaCI CACGTG 1 cut(s) 536
PmlI CACGTG 1 cut(s) 536
PpsI GAGTC 4 cut(s) 179, 562, 1238, 1488
Ppu21I YACGTR 1 cut(s) 536
PpuMI RGGWCCY 1 cut(s) 249
Psp124BI GAGCTC 1 cut(s) 413
Psp5II RGGWCCY 1 cut(s) 249
Psp6I CCWGG 1 cut(s) 631
PspCI CACGTG 1 cut(s) 536
PspFI CCCAGC 2 cut(s) 252, 1049
PspGI CCWGG 1 cut(s) 631
PspN4I GGNNCC 8 cut(s) 250, 251, 308, 309, 471, 519, 630, 1054
PspOMI GGGCCC 1 cut(s) 307
PspPPI RGGWCCY 1 cut(s) 249
PstI CTGCAG 2 cut(s) 730, 1477
PsuI RGATCY 1 cut(s) 1289
RseI CAYNNNNRTG 1 cut(s) 1205
SacI GAGCTC 1 cut(s) 413
SacII CCGCGG 1 cut(s) 354
SalI GTCGAC 1 cut(s) 488
SaqAI TTAA 2 cut(s) 1089, 1317
Sau3AI GATC 4 cut(s) 327, 524, 1289, 1305
SbfI CCTGCAGG 1 cut(s) 1477
SchI GAGTC 4 cut(s) 179, 562, 1238, 1489
ScrFI CCNGG 4 cut(s) 315, 587, 633, 826
SdaI CCTGCAGG 1 cut(s) 1477
SduI GDGCHC 5 cut(s) 278, 311, 413, 916, 1222
SfaNI GCATC 4 cut(s) 724, 837, 1134, 1346
SfcI CTRYAG 4 cut(s) 726, 1032, 1473, 1560
Sfr303I CCGCGG 1 cut(s) 354
SgrBI CCGCGG 1 cut(s) 354
SgrDI CGTCGACG 1 cut(s) 488
SinI GGWCC 4 cut(s) 249, 469, 673, 1053
SmiMI CAYNNNNRTG 1 cut(s) 1205
SmlI CTYRAG 1 cut(s) 570
SmoI CTYRAG 1 cut(s) 570
SpeI ACTAGT 1 cut(s) 437
Sse8387I CCTGCAGG 1 cut(s) 1477
Sse9I AATT 7 cut(s) 750, 816, 928, 966, 1155, 1466, 1572
SseBI AGGCCT 1 cut(s) 52
SspI AATATT 2 cut(s) 1443, 1669
SspMI CTAG 3 cut(s) 438, 743, 917
SstI GAGCTC 1 cut(s) 413
StuI AGGCCT 1 cut(s) 52
StyD4I CCNGG 4 cut(s) 313, 585, 631, 824
StyI CCWWGG 4 cut(s) 425, 562, 771, 1621
TaaI ACNGT 2 cut(s) 1087, 1495
TaiI ACGT 2 cut(s) 299, 538
TaqI TCGA 5 cut(s) 450, 489, 1326, 1422, 1483
TasI AATT 7 cut(s) 750, 816, 928, 966, 1155, 1466, 1572
TauI GCSGC 9 cut(s) 92, 211, 259, 287, 337, 372, 669, 853, 856
TfiI GAWTC 3 cut(s) 1234, 1263, 1419
Tru1I TTAA 2 cut(s) 1089, 1317
Tru9I TTAA 2 cut(s) 1089, 1317
TscAI CASTG 3 cut(s) 800, 1144, 1414
TseFI GTSAC 2 cut(s) 292, 894
TseI GCWGC 8 cut(s) 233, 259, 725, 728, 737, 746, 1516, 1610
Tsp45I GTSAC 2 cut(s) 292, 894
TspDTI ATGAA 1 cut(s) 882
TspGWI ACGGA 1 cut(s) 368
TspRI CASTG 3 cut(s) 800, 1144, 1414
Van91I CCANNNNNTGG 1 cut(s) 986
VpaK11BI GGWCC 4 cut(s) 249, 469, 673, 1053
XapI RAATTY 2 cut(s) 1466, 1572
XbaI TCTAGA 1 cut(s) 916
XceI RCATGY 1 cut(s) 1391
XcmI CCANNNNNNNNNTGG 2 cut(s) 1063, 1144
XmiI GTMKAC 1 cut(s) 489
XspI CTAG 3 cut(s) 438, 743, 917
ZraI GACGTC 1 cut(s) 297
Zsp2I ATGCAT 1 cut(s) 1012
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.