Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0004091

Physical Location & Seq

Genomic Coordinates Forward (+)

27338332 .. 27342086

Gene Structure 3755 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1210000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2964 bp

ATGTCGTCATTGACAGACCGGAGAAGAACCCATCTCCCGCTCGACCTCCTCTTCCTCCTCCTCTGCCTCCTCTCCACCGCGGCGGCCGACGAGCTTCAAATTCTTCTCAAAATCAAATCCACGTTACAAGTTTCAAACACCAACAACAACATATTCAATACATGGAACTCCACCAATTCCGTTTGCAGCTTCACCGGAATTGCCTGCATCGAAAACGGATTCGTTCGAGATATCGACCTTTCGAATCAGAACCTATCTGGGTTTCTTGCGGCGGACGCAATATGCCAGCTCGAATCGTTGGAAACACTTTCCTTGGGATTCAACTTCTTGCACGGGACAATCAAGGAGGACTTGAACAACTGCGTAAATTTGAAGTACTTGGACTTGGGCAACAATTTGTTCTCAGGATCGTTCCCCGACATATCATCCCTGTCCCAGTTAGAACATCTCCATCTGAACTGCAGCGGATTTTCGGGTACTTTTCCGTGGAAATCATTGGGGAACATGACGGGTTTGATTCGATTGAGCTTGGGAGACAACCCTTTTGATCAAAGCCCATTCCCAACCGAGGTGTTGAATCTCAACAAACTCAACTGGCTTTACTTGTCGAATTGCAGCCTCGGAGGTTCGATTCCGAAAGGAATCGGAAACCTCACGGAGCTTATCAACTTGGAGTTATCCGACAACAGCATGGTCGGAGAAATCCCATCCGAGATTACAAAGCTTACCAAGCTCTGGCAGCTCGAGCTCTACCACAACCAGTTCACCGGGACGCTTCCTCCCGGCCTAAGAAATCTCACCAACCTCGAAAACTTTGACGCCTCCATGAATCTACTCGAAGGCGATTTGTCGGAGGTGGGATTCTTGGAGAACATAGTTTCCCTCCAATTGTACAAGAATAGTTTCTCCGGAGAAGTTCCGATGGAGTTCGGGGAATTCAAGAAGCTTGTCAATCTGTCGTTGTACAATAACAAGCTGACCGGTCCTCTGCCTCAGAAACTTGGCTCTTGGTCCAAGATTGATTTCATCGATATCTCTGAGAATCTCCTGACGGGAAGTATTCCGCCCGATATGTGCAAGATGGGGACGATGAGAGGCTTTCTCCTGCTCCAGAACAAGTTTACCGGCGAAATTCCTGCGAATTACGCTAAGTGTTCCACATTGAAGCGGTTCAGAGTCAACATCAACTCGCTGTCCGGTGTTGTTCCTCCTGGAATTTGGGGGTTGCCGAATGCGCACATCATCGACCTTACTTCCAATCAATTCGAAGGCCCGATAACTTCTGACATTGGAAATGCAAAGATGCTTGCGCAGTTGTTCGTCAGTTACAATCAGTTATCTGGTGAGCTGCCGGATGAGATTTCCAAAGCAACATCTTTGGTGTCCATTGTTTTGAACAACAATCGGTTTTCCGGGAACGTCCCTGGAAGTGTTGGTGACCTGACGCATCTGGGAACTCTGTATTTGCAGAGCAACATGTTCTCTGCTTCGATACCAGAGTCTCTAGGAAACTGTAATTTACTGAGCGACTTAAACATGGCAGAAAACTCATTTTCCGGTGACATCCCATCGTCTTTAGGCTCTCTCCCAACCTTGAACTCTCTGAATTTGTCACAAAATCAACTTTCGGGTGAAATCCCAAAGAGCTTAGCATCTCTAAGGCTAAGTCTTCTTGATCTATCACACAACAGGCTCACCGGCGCCATACCAGAATCTCTGTCGATTGCAGCCTACAACGGTAGCTTTTCTGGTAACTCCGGACTCTGCAGCACGGACATAAGCCCATTCCCCCATTGTTCTGCTGGTTCTGGGATGTCCAAGGATGTGCGGACGCTAATTATTTGCTTCTCGGCTGGCTCAGCAATTCTGCTTGTGTCCCTCACATGCTTCTTGTTCTTAAAGAAGCGCGAAAAGGACGAAGACCGTTCGTTAAAGGAGGAATCTTGGGACGTAAACTCTTTTCATGTGATGAGCTTCACCGAGGGTGAAATTCTTGATTCCATTAGGCAAGAGAATCTTATTGGAAAAGGGGGTTCCGGAAACGTTTACAGAGTTTTACTTGCGAATGGCAAAGATCTCGCTGTAAAGCACATATGGAACACCGATCCAAGTGACAGGAAAAGGTTCAAGAGCATGGCTCCAGTGCTTGCAAAACGCGGTGGGAAGTCGAAGGAATTTAATGCTGAGGTGCAGACGTTGAGCTCGATTAGGCATGTGAATGTGGTGAAGTTGTACTGTAGCATTACAAGCGAGGACTCAAGCTTGTTGGTGTATGAGTACATGCCGAATGGTAGCTTGTGGGATCAGCTTCATACGTGCCAAAAGATGAAGCTTGATTGGGAGACAAGGCATGAGATAGCAGTGGGAGCAGCCAAAGGTTTGGAGTATTTGCATCATGGCTTGGAGAGGCTGGTGATGCACCGGGATGTCAAGTCGAGTAACATTTTATTGGATGAGTTTTTGAAGCCGAAGATTGCGGATTTCGGGCTTGCCAAGATTGTTCAGGCCACTGCAGACAAGGACTCTACTCATGTTATTGCCGGAACACATGGCTACATTGCTCCTGAATATGGATACACGTACAAAGTGAATGAGAAGAGCGATGTGTACAGCTTCGGAGTGGTACTAATGGAGCTAGTGACGGGGAAAAGGCCGATAGAACCAGAGTTCGGAGAGAACAAGGATATAGTGAGCTGGGTAAGCAGCATGCTTGGGAGTAGAGAGAGCATATTAAGCATGGTGGACTCATATATTCCAGAGGTGTACAAAGAAGAGGCTATCAAGGTTTTAAGAATTGCAATTCTCTGCACGGCTAGGGTACCGGAGTTAAGACCCTCCATGAGAAGTGTGGTTCAAATGCTTGAAGAGGCTCACGAGACATTTAAATTGCTCAAGATTGTTATCAGCAAAGATGGTGCTGCTGCAGATATGAAAATGAAAGTAGTGAAGGGTATAGAGAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001653 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009791 GO:0009845 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045087 GO:0046777 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0090351 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

988

Amino Acids

108.6

Weight (kDa)

5.61

Isoelectric Point (pI)

33.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	29 - 69	3e-07	Leucine rich repeat N-terminal domain
LRR_14	PF23598	189 - 274	6.4e-10	Leucine-rich repeat region
LRR_14	PF23598	427 - 552	4.1e-07	Leucine-rich repeat region
Pkinase	PF00069	670 - 952	1.3e-43	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	671 - 954	4.9e-38	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0004091)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09970 AT1G09970
fragaria_vesca	FvH4_5g33590
malus_domestica	MD08G1210000.v1.1 MD15G1394100.v1.1
prunus_persica	Prupe.1G542300_v2.0.a1
pyrus_communis	pycom08g18120 pycom15g35400 pycom15g35410
rosa_chinensis	RchiOBHm_Chr7g0217331 RchiOBHm_Chr7g0232471
rosa_laevigata	RLG00000001335 RLG00000002489 RLG00000002493
rosa_multiflora	Rmu_sc0001898.1_g000015 Rmu_sc0004663.1_g000011 Rmu_sc0011824.1_g000016
rosa_roxburghii	Rroxscaffold_3G00228280 Rroxscaffold_3G00242430
rosa_rugosa	Rorug07G0168100 Rorug07G0274700
rosa_samantha	Rh7AG307400 Rh7AG307800 Rh7AG428100 Rh7BG298200 Rh7BG402600 Rh7CG326500 Rh7CG326900 Rh7CG447100 Rh7DG306900 Rh7DG418900
rosa_wichuraiana	Rw7G026090 Rw7G035470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	2 cut(s)	1236, 1311
Acc65I	GGTACC	1 cut(s)	2818
AccB1I	GGYRCC	2 cut(s)	1700, 2818
AccB7I	CCANNNNNTGG	1 cut(s)	735
AccBSI	CCGCTC	1 cut(s)	40
AccII	CGCG	3 cut(s)	80, 1908, 2157
AccIII	TCCGGA	3 cut(s)	908, 1757, 2036
AclI	AACGTT	1 cut(s)	2043
AclWI	GGATC	3 cut(s)	415, 2099, 2310
AcoI	YGGCCR	1 cut(s)	84
AcsI	RAATTY	8 cut(s)	99, 367, 935, 1131, 1215, 1606, 1989, 2174
AcyI	GRCGYC	2 cut(s)	819, 1701
AfiI	CCNNNNNNNGG	4 cut(s)	568, 735, 2570, 2669
AflIII	ACRYGT	2 cut(s)	1476, 2577
AgeI	ACCGGT	1 cut(s)	980
AjnI	CCWGG	2 cut(s)	1210, 1423
AjuI	GAANNNNNNNTTGG	2 cut(s)	1007, 1039
AloI	GAACNNNNNNTCC	2 cut(s)	863, 895
Alw21I	GWGCWC	2 cut(s)	750, 2204
Alw26I	GTCTC	4 cut(s)	528, 1506, 2336, 2870
AlwI	GGATC	3 cut(s)	415, 2099, 2310
Ama87I	CYCGRG	1 cut(s)	743
Aor13HI	TCCGGA	3 cut(s)	908, 1757, 2036
AoxI	GGCC	5 cut(s)	84, 784, 1270, 2505, 2651
ApoI	RAATTY	8 cut(s)	99, 367, 935, 1131, 1215, 1606, 1989, 2174
ArsI	GACNNNNNNTTYG	2 cut(s)	527, 559
AsiGI	ACCGGT	1 cut(s)	980
Asp700I	GAANNNNTTC	3 cut(s)	902, 1169, 2123
Asp718I	GGTACC	1 cut(s)	2818
AspLEI	GCGC	4 cut(s)	1237, 1312, 1703, 1908
AspS9I	GGNCC	3 cut(s)	983, 1011, 1271
AsuC2I	CCSGG	4 cut(s)	769, 783, 1414, 2423
AsuII	TTCGAA	2 cut(s)	242, 1266
AvaI	CYCGRG	1 cut(s)	743
AvaII	GGWCC	2 cut(s)	983, 1011
BaeI	ACNNNNGTAYC	4 cut(s)	1484, 1517, 2810, 2843
BanI	GGYRCC	2 cut(s)	1700, 2818
BanII	GRGCYC	2 cut(s)	750, 2204
BauI	CACGAG	1 cut(s)	2873
BbsI	GAAGAC	2 cut(s)	1661, 1926
Bbv12I	GWGCWC	2 cut(s)	750, 2204
BbvCI	CCTCAGC	1 cut(s)	2184
BccI	CCATC	7 cut(s)	39, 459, 715, 916, 1075, 1576, 2906
BceAI	ACGGC	1 cut(s)	2826
BcgI	CGANNNNNNTGC	4 cut(s)	1118, 1152, 2059, 2093
BciT130I	CCWGG	2 cut(s)	1212, 1425
BciVI	GTATCC	1 cut(s)	2567
BclI	TGATCA	1 cut(s)	547
BcnI	CCSGG	4 cut(s)	769, 783, 1414, 2423
BcoDI	GTCTC	4 cut(s)	528, 1506, 2336, 2870
BfaI	CTAG	3 cut(s)	1505, 2636, 2814
BfmI	CTRYAG	5 cut(s)	460, 1765, 2236, 2511, 2922
BfoI	RGCGCY	1 cut(s)	1704
BfuI	GTATCC	1 cut(s)	2567
BglII	AGATCT	1 cut(s)	2074
BlpI	GCTNAGC	2 cut(s)	1648, 1858
BmcAI	AGTACT	1 cut(s)	377
Bme1390I	CCNGG	6 cut(s)	769, 783, 1212, 1414, 1425, 2423
Bme18I	GGWCC	2 cut(s)	983, 1011
BmeT110I	CYCGRG	1 cut(s)	743
BmgT120I	GGNCC	3 cut(s)	983, 1011, 1271
BmiI	GGNNCC	4 cut(s)	1702, 2035, 2139, 2820
BmrFI	CCNGG	6 cut(s)	769, 783, 1212, 1414, 1425, 2423
BmrI	ACTGGG	1 cut(s)	430
BmsI	GCATC	6 cut(s)	216, 1293, 1456, 1661, 2401, 2406
BmuI	ACTGGG	1 cut(s)	430
BpiI	GAAGAC	2 cut(s)	1661, 1926
BplI	GAGNNNNNCTC	4 cut(s)	1086, 1118, 2122, 2154
BpmI	CTGGAG	2 cut(s)	1094, 2124
Bpu10I	CCTNAGC	1 cut(s)	2184
Bpu1102I	GCTNAGC	2 cut(s)	1648, 1858
Bpu14I	TTCGAA	2 cut(s)	242, 1266
BpuEI	CTTGAG	2 cut(s)	2242, 2876
BpuMI	CCSGG	4 cut(s)	769, 783, 1414, 2423
Bsa29I	ATCGAT	1 cut(s)	1029
BsaAI	YACGTR	2 cut(s)	2316, 2580
BsaBI	GATNNNNATC	1 cut(s)	2900
BsaHI	GRCGYC	2 cut(s)	819, 1701
BsaJI	CCNNGG	8 cut(s)	78, 312, 485, 567, 619, 1423, 1818, 1980
BsaWI	WCCGGW	9 cut(s)	18, 194, 908, 980, 1196, 1556, 1757, 2036, 2821
BsaXI	ACNNNNNCTCC	2 cut(s)	763, 793
Bsc4I	CCNNNNNNNGG	4 cut(s)	568, 735, 2570, 2669
Bse118I	RCCGGY	3 cut(s)	980, 1124, 1697
Bse1I	ACTGG	4 cut(s)	436, 599, 760, 2141
Bse3DI	GCAATG	1 cut(s)	2556
Bse8I	GATNNNNATC	1 cut(s)	2900
BseAI	TCCGGA	3 cut(s)	908, 1757, 2036
BseBI	CCWGG	2 cut(s)	1212, 1425
BseCI	ATCGAT	1 cut(s)	1029
BseDI	CCNNGG	8 cut(s)	78, 312, 485, 567, 619, 1423, 1818, 1980
BseGI	GGATG	8 cut(s)	425, 707, 1360, 1563, 1818, 1828, 2431, 2458
BseJI	GATNNNNATC	1 cut(s)	2900
BseLI	CCNNNNNNNGG	4 cut(s)	568, 735, 2570, 2669
BseMI	GCAATG	1 cut(s)	2556
BseMII	CTCAG	6 cut(s)	417, 1007, 1029, 1514, 1872, 2175
BseNI	ACTGG	4 cut(s)	436, 599, 760, 2141
BseRI	GAGGAG	4 cut(s)	38, 47, 50, 59
BseX3I	CGGCCG	1 cut(s)	84
BseYI	CCCAGC	1 cut(s)	2694
BsgI	GTGCAG	2 cut(s)	2210, 2791
Bsh1236I	CGCG	3 cut(s)	80, 1908, 2157
Bsh1285I	CGRYCG	1 cut(s)	87
BshFI	GGCC	5 cut(s)	86, 786, 1272, 2507, 2653
BshNI	GGYRCC	2 cut(s)	1700, 2818
BshTI	ACCGGT	1 cut(s)	980
BshVI	ATCGAT	1 cut(s)	1029
BsiEI	CGRYCG	1 cut(s)	87
BsiHKAI	GWGCWC	2 cut(s)	750, 2204
BsiHKCI	CYCGRG	1 cut(s)	743
BslFI	GGGAC	7 cut(s)	349, 418, 784, 1099, 1406, 1861, 1961
BslI	CCNNNNNNNGG	4 cut(s)	568, 735, 2570, 2669
BsmAI	GTCTC	4 cut(s)	528, 1506, 2336, 2870
BsmFI	GGGAC	7 cut(s)	349, 418, 784, 1099, 1406, 1861, 1961
BsmI	GAATGC	1 cut(s)	1237
BsnI	GGCC	5 cut(s)	86, 786, 1272, 2507, 2653
BsoBI	CYCGRG	1 cut(s)	743
Bsp119I	TTCGAA	2 cut(s)	242, 1266
Bsp1286I	GDGCHC	2 cut(s)	750, 2204
Bsp13I	TCCGGA	3 cut(s)	908, 1757, 2036
Bsp1407I	TGTACA	4 cut(s)	891, 963, 2607, 2763
Bsp143I	GATC	6 cut(s)	407, 547, 1675, 2074, 2104, 2302
Bsp1720I	GCTNAGC	2 cut(s)	1648, 1858
BspANI	GGCC	5 cut(s)	86, 786, 1272, 2507, 2653
BspCNI	CTCAG	6 cut(s)	416, 1006, 1030, 1515, 1871, 2176
BspDI	ATCGAT	1 cut(s)	1029
BspEI	TCCGGA	3 cut(s)	908, 1757, 2036
BspFNI	CGCG	3 cut(s)	80, 1908, 2157
BspLI	GGNNCC	4 cut(s)	1702, 2035, 2139, 2820
BspMAI	CTGCAG	4 cut(s)	464, 1769, 2515, 2926
BspPI	GGATC	3 cut(s)	415, 2099, 2310
BspQI	GCTCTTC	1 cut(s)	2591
BspT104I	TTCGAA	2 cut(s)	242, 1266
BspT107I	GGYRCC	2 cut(s)	1700, 2818
BsrBI	CCGCTC	1 cut(s)	40
BsrDI	GCAATG	1 cut(s)	2556
BsrFI	RCCGGY	3 cut(s)	980, 1124, 1697
BsrGI	TGTACA	4 cut(s)	891, 963, 2607, 2763
BsrI	ACTGG	4 cut(s)	436, 599, 760, 2141
BssAI	RCCGGY	3 cut(s)	980, 1124, 1697
BssECI	CCNNGG	8 cut(s)	78, 312, 485, 567, 619, 1423, 1818, 1980
BssMI	GATC	6 cut(s)	407, 547, 1675, 2074, 2104, 2302
BssNI	GRCGYC	2 cut(s)	819, 1701
BssSI	CACGAG	1 cut(s)	2873
BssT1I	CCWWGG	2 cut(s)	312, 1818
Bst2BI	CACGAG	1 cut(s)	2873
Bst2UI	CCWGG	2 cut(s)	1212, 1425
Bst4CI	ACNGT	4 cut(s)	1514, 1739, 1925, 2237
Bst6I	CTCTTC	4 cut(s)	56, 2591, 2766, 2859
BstACI	GRCGYC	2 cut(s)	819, 1701
BstAUI	TGTACA	4 cut(s)	891, 963, 2607, 2763
BstBAI	YACGTR	2 cut(s)	2316, 2580
BstBI	TTCGAA	2 cut(s)	242, 1266
BstC8I	GCNNGC	7 cut(s)	205, 287, 1308, 1855, 2148, 2490, 2708
BstDSI	CCRYGG	2 cut(s)	78, 485
BstEII	GGTNACC	1 cut(s)	1436
BstF5I	GGATG	8 cut(s)	425, 707, 1360, 1563, 1818, 1828, 2431, 2458
BstFNI	CGCG	3 cut(s)	80, 1908, 2157
BstH2I	RGCGCY	1 cut(s)	1704
BstHHI	GCGC	4 cut(s)	1237, 1312, 1703, 1908
BstKTI	GATC	6 cut(s)	410, 550, 1678, 2077, 2107, 2305
BstMAI	GTCTC	4 cut(s)	528, 1506, 2336, 2870
BstMBI	GATC	6 cut(s)	407, 547, 1675, 2074, 2104, 2302
BstMCI	CGRYCG	1 cut(s)	87
BstNI	CCWGG	2 cut(s)	1212, 1425
BstNSI	RCATGY	5 cut(s)	1480, 1887, 2216, 2284, 2710
BstPI	GGTNACC	1 cut(s)	1436
BstSCI	CCNGG	6 cut(s)	767, 781, 1210, 1412, 1423, 2421
BstSFI	CTRYAG	5 cut(s)	460, 1765, 2236, 2511, 2922
BstUI	CGCG	3 cut(s)	80, 1908, 2157
BstV2I	GAAGAC	2 cut(s)	1661, 1926
BstX2I	RGATCY	1 cut(s)	2074
BstXI	CCANNNNNNTGG	1 cut(s)	2380
BstYI	RGATCY	1 cut(s)	2074
BstZI	CGGCCG	1 cut(s)	84
Bsu15I	ATCGAT	1 cut(s)	1029
BsuI	GTATCC	1 cut(s)	2567
BsuRI	GGCC	5 cut(s)	86, 786, 1272, 2507, 2653
BsuTUI	ATCGAT	1 cut(s)	1029
BtgI	CCRYGG	2 cut(s)	78, 485
BtgZI	GCGATG	1 cut(s)	2616
BtsCI	GGATG	8 cut(s)	425, 707, 1360, 1563, 1818, 1828, 2431, 2458
BtsI	GCAGTG	2 cut(s)	2367, 2508
BtsIMutI	CAGTG	3 cut(s)	2148, 2367, 2508
Cac8I	GCNNGC	7 cut(s)	205, 287, 1308, 1855, 2148, 2490, 2708
CfoI	GCGC	4 cut(s)	1237, 1312, 1703, 1908
Cfr10I	RCCGGY	3 cut(s)	980, 1124, 1697
Cfr13I	GGNCC	3 cut(s)	983, 1011, 1271
Cfr42I	CCGCGG	1 cut(s)	81
ClaI	ATCGAT	1 cut(s)	1029
CseI	GACGC	5 cut(s)	284, 781, 827, 1453, 1840
CspAI	ACCGGT	1 cut(s)	980
DinI	GGCGCC	1 cut(s)	1702
DpnI	GATC	6 cut(s)	409, 549, 1677, 2076, 2106, 2304
DpnII	GATC	6 cut(s)	407, 547, 1675, 2074, 2104, 2302
DraI	TTTAAA	1 cut(s)	2884
EaeI	YGGCCR	1 cut(s)	84
EagI	CGGCCG	1 cut(s)	84
Eam1104I	CTCTTC	4 cut(s)	56, 2591, 2766, 2859
EarI	CTCTTC	4 cut(s)	56, 2591, 2766, 2859
EciI	GGCGGA	2 cut(s)	287, 1053
Ecl136II	GAGCTC	2 cut(s)	748, 2202
EclXI	CGGCCG	1 cut(s)	84
Eco130I	CCWWGG	2 cut(s)	312, 1818
Eco24I	GRGCYC	2 cut(s)	750, 2204
Eco32I	GATATC	2 cut(s)	232, 1033
Eco47I	GGWCC	2 cut(s)	983, 1011
Eco52I	CGGCCG	1 cut(s)	84
Eco53kI	GAGCTC	2 cut(s)	748, 2202
Eco88I	CYCGRG	1 cut(s)	743
Eco91I	GGTNACC	1 cut(s)	1436
EcoICRI	GAGCTC	2 cut(s)	748, 2202
EcoO65I	GGTNACC	1 cut(s)	1436
EcoRI	GAATTC	1 cut(s)	935
EcoRII	CCWGG	2 cut(s)	1210, 1423
EcoRV	GATATC	2 cut(s)	232, 1033
EcoT14I	CCWWGG	2 cut(s)	312, 1818
EcoT38I	GRGCYC	2 cut(s)	750, 2204
EgeI	GGCGCC	1 cut(s)	1702
EheI	GGCGCC	1 cut(s)	1702
ErhI	CCWWGG	2 cut(s)	312, 1818
FalI	AAGNNNNNCTT	4 cut(s)	335, 367, 2001, 2033
FaqI	GGGAC	7 cut(s)	349, 418, 784, 1099, 1406, 1861, 1961
FauI	CCCGC	1 cut(s)	45
FauNDI	CATATG	1 cut(s)	2093
FbaI	TGATCA	1 cut(s)	547
FokI	GGATG	8 cut(s)	412, 694, 1367, 1550, 1825, 1835, 2438, 2465
FriOI	GRGCYC	2 cut(s)	750, 2204
FspAI	RTGCGCAY	1 cut(s)	1236
FspBI	CTAG	3 cut(s)	1505, 2636, 2814
FspI	TGCGCA	2 cut(s)	1236, 1311
GlaI	GCGC	4 cut(s)	1236, 1311, 1702, 1907
GsaI	CCCAGC	1 cut(s)	2698
GsuI	CTGGAG	2 cut(s)	1094, 2124
HaeII	RGCGCY	1 cut(s)	1704
HaeIII	GGCC	5 cut(s)	86, 786, 1272, 2507, 2653
HgaI	GACGC	5 cut(s)	284, 781, 827, 1453, 1840
HhaI	GCGC	4 cut(s)	1237, 1312, 1703, 1908
Hin1I	GRCGYC	2 cut(s)	819, 1701
Hin6I	GCGC	4 cut(s)	1235, 1310, 1701, 1906
HinP1I	GCGC	4 cut(s)	1235, 1310, 1701, 1906
HincII	GTYRAC	1 cut(s)	1180
HindII	GTYRAC	1 cut(s)	1180
HindIII	AAGCTT	4 cut(s)	722, 944, 2260, 2330
Hpy166II	GTNNAC	8 cut(s)	765, 1122, 1180, 1954, 2047, 2608, 2743, 2764
Hpy8I	GTNNAC	8 cut(s)	765, 1122, 1180, 1954, 2047, 2608, 2743, 2764
Hpy99I	CGWCG	1 cut(s)	92
HpyAV	CCTTC	4 cut(s)	833, 1262, 2164, 2941
HpyCH4III	ACNGT	4 cut(s)	1514, 1739, 1925, 2237
HpyCH4IV	ACGT	7 cut(s)	122, 1419, 1950, 2043, 2195, 2315, 2579
HpySE526I	ACGT	7 cut(s)	122, 1419, 1950, 2043, 2195, 2315, 2579
Hsp92I	GRCGYC	2 cut(s)	819, 1701
HspAI	GCGC	4 cut(s)	1235, 1310, 1701, 1906
KasI	GGCGCC	1 cut(s)	1700
Kpn2I	TCCGGA	3 cut(s)	908, 1757, 2036
KpnI	GGTACC	1 cut(s)	2822
Ksp22I	TGATCA	1 cut(s)	547
KspI	CCGCGG	1 cut(s)	81
Kzo9I	GATC	6 cut(s)	407, 547, 1675, 2074, 2104, 2302
LguI	GCTCTTC	1 cut(s)	2591
LmnI	GCTCC	6 cut(s)	658, 1113, 2143, 2366, 2566, 2632
LweI	GCATC	6 cut(s)	216, 1293, 1456, 1661, 2401, 2406
MaeI	CTAG	3 cut(s)	1505, 2636, 2814
MaeII	ACGT	7 cut(s)	122, 1419, 1950, 2043, 2195, 2315, 2579
MaeIII	GTNAC	9 cut(s)	123, 1325, 1436, 1559, 1611, 1751, 2111, 2438, 2638
MalI	GATC	6 cut(s)	409, 549, 1677, 2076, 2106, 2304
MbiI	CCGCTC	1 cut(s)	40
MboI	GATC	6 cut(s)	407, 547, 1675, 2074, 2104, 2302
MboII	GAAGA	9 cut(s)	36, 43, 95, 1661, 1931, 2482, 2608, 2783, 2876
MfeI	CAATTG	1 cut(s)	887
MflI	RGATCY	1 cut(s)	2074
MhlI	GDGCHC	2 cut(s)	750, 2204
Mly113I	GGCGCC	1 cut(s)	1701
MlyI	GAGTC	6 cut(s)	1185, 1508, 1755, 2249, 2516, 2738
MmeI	TCCRAC	4 cut(s)	279, 676, 705, 831
MroI	TCCGGA	3 cut(s)	908, 1757, 2036
MroXI	GAANNNNTTC	3 cut(s)	902, 1169, 2123
MseI	TTAA	8 cut(s)	1532, 1898, 1931, 2178, 2732, 2789, 2828, 2883
MslI	CAYNNNNRTG	2 cut(s)	2217, 2424
MspA1I	CMGCKG	2 cut(s)	80, 465
MspR9I	CCNGG	6 cut(s)	769, 783, 1212, 1414, 1425, 2423
MunI	CAATTG	1 cut(s)	887
Mva1269I	GAATGC	1 cut(s)	1237
MvaI	CCWGG	2 cut(s)	1212, 1425
MvnI	CGCG	3 cut(s)	80, 1908, 2157
NarI	GGCGCC	1 cut(s)	1701
NciI	CCSGG	4 cut(s)	769, 783, 1414, 2423
NdeI	CATATG	1 cut(s)	2093
NdeII	GATC	6 cut(s)	407, 547, 1675, 2074, 2104, 2302
NlaIV	GGNNCC	4 cut(s)	1702, 2035, 2139, 2820
NmeAIII	GCCGAG	1 cut(s)	1829
NmuCI	GTSAC	5 cut(s)	1436, 1559, 1611, 2111, 2638
NsbI	TGCGCA	2 cut(s)	1236, 1311
NspI	RCATGY	5 cut(s)	1480, 1887, 2216, 2284, 2710
NspV	TTCGAA	2 cut(s)	242, 1266
PaeI	GCATGC	1 cut(s)	2710
PaeR7I	CTCGAG	1 cut(s)	743
PciI	ACATGT	1 cut(s)	1476
PciSI	GCTCTTC	1 cut(s)	2591
PcsI	WCGNNNNNNNCGW	1 cut(s)	2201
PctI	GAATGC	1 cut(s)	1237
PdmI	GAANNNNTTC	3 cut(s)	902, 1169, 2123
PflMI	CCANNNNNTGG	1 cut(s)	735
PfoI	TCCNGGA	2 cut(s)	1210, 1412
PinAI	ACCGGT	1 cut(s)	980
PleI	GAGTC	6 cut(s)	1184, 1507, 1755, 2249, 2516, 2738
PluTI	GGCGCC	1 cut(s)	1704
PpsI	GAGTC	6 cut(s)	1184, 1507, 1755, 2249, 2516, 2738
Ppu21I	YACGTR	2 cut(s)	2316, 2580
PscI	ACATGT	1 cut(s)	1476
Psp124BI	GAGCTC	2 cut(s)	750, 2204
Psp1406I	AACGTT	1 cut(s)	2043
Psp6I	CCWGG	2 cut(s)	1210, 1423
PspEI	GGTNACC	1 cut(s)	1436
PspFI	CCCAGC	1 cut(s)	2694
PspGI	CCWGG	2 cut(s)	1210, 1423
PspN4I	GGNNCC	4 cut(s)	1702, 2035, 2139, 2820
PspPI	GGNCC	3 cut(s)	983, 1011, 1271
PspXI	VCTCGAGB	1 cut(s)	743
PstI	CTGCAG	4 cut(s)	464, 1769, 2515, 2926
PsuI	RGATCY	1 cut(s)	2074
RseI	CAYNNNNRTG	2 cut(s)	2217, 2424
SacI	GAGCTC	2 cut(s)	750, 2204
SacII	CCGCGG	1 cut(s)	81
SapI	GCTCTTC	1 cut(s)	2591
SaqAI	TTAA	8 cut(s)	1532, 1898, 1931, 2178, 2732, 2789, 2828, 2883
Sau3AI	GATC	6 cut(s)	407, 547, 1675, 2074, 2104, 2302
Sau96I	GGNCC	3 cut(s)	983, 1011, 1271
ScaI	AGTACT	1 cut(s)	377
SchI	GAGTC	6 cut(s)	1185, 1508, 1755, 2249, 2516, 2738
ScrFI	CCNGG	6 cut(s)	769, 783, 1212, 1414, 1425, 2423
SduI	GDGCHC	2 cut(s)	750, 2204
SfaNI	GCATC	6 cut(s)	216, 1293, 1456, 1661, 2401, 2406
SfcI	CTRYAG	5 cut(s)	460, 1765, 2236, 2511, 2922
SfoI	GGCGCC	1 cut(s)	1702
Sfr274I	CTCGAG	1 cut(s)	743
Sfr303I	CCGCGG	1 cut(s)	81
SfuI	TTCGAA	2 cut(s)	242, 1266
SgrAI	CRCCGGYG	1 cut(s)	1697
SgrBI	CCGCGG	1 cut(s)	81
SinI	GGWCC	2 cut(s)	983, 1011
SlaI	CTCGAG	1 cut(s)	743
SmiI	ATTTAAAT	1 cut(s)	2884
SmiMI	CAYNNNNRTG	2 cut(s)	2217, 2424
SmlI	CTYRAG	3 cut(s)	743, 2257, 2891
SmoI	CTYRAG	3 cut(s)	743, 2257, 2891
SphI	GCATGC	1 cut(s)	2710
SspDI	GGCGCC	1 cut(s)	1700
SspMI	CTAG	3 cut(s)	1505, 2636, 2814
SstI	GAGCTC	2 cut(s)	750, 2204
StyD4I	CCNGG	6 cut(s)	767, 781, 1210, 1412, 1423, 2421
StyI	CCWWGG	2 cut(s)	312, 1818
SwaI	ATTTAAAT	1 cut(s)	2884
TaaI	ACNGT	4 cut(s)	1514, 1739, 1925, 2237
TaiI	ACGT	7 cut(s)	125, 1422, 1953, 2046, 2198, 2318, 2582
TatI	WGTACW	7 cut(s)	375, 891, 963, 2232, 2277, 2607, 2763
TauI	GCSGC	3 cut(s)	83, 86, 272
Tru1I	TTAA	8 cut(s)	1532, 1898, 1931, 2178, 2732, 2789, 2828, 2883
Tru9I	TTAA	8 cut(s)	1532, 1898, 1931, 2178, 2732, 2789, 2828, 2883
TscAI	CASTG	3 cut(s)	2148, 2367, 2515
TseFI	GTSAC	5 cut(s)	1436, 1559, 1611, 2111, 2638
Tsp45I	GTSAC	5 cut(s)	1436, 1559, 1611, 2111, 2638
TspDTI	ATGAA	7 cut(s)	842, 1015, 1952, 2300, 2342, 2945, 2951
TspGWI	ACGGA	5 cut(s)	169, 231, 474, 671, 1787
TspRI	CASTG	3 cut(s)	2148, 2367, 2515
Van91I	CCANNNNNTGG	1 cut(s)	735
VpaK11BI	GGWCC	2 cut(s)	983, 1011
XapI	RAATTY	8 cut(s)	99, 367, 935, 1131, 1215, 1606, 1989, 2174
XceI	RCATGY	5 cut(s)	1480, 1887, 2216, 2284, 2710
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1799, 2845
XhoI	CTCGAG	1 cut(s)	743
XmnI	GAANNNNTTC	3 cut(s)	902, 1169, 2123
XspI	CTAG	3 cut(s)	1505, 2636, 2814
ZrmI	AGTACT	1 cut(s)	377

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.