Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7A

Orthogroup ID

OG0004091

Physical Location & Seq

Genomic Coordinates Reverse (-)

57531523 .. 57534736

Gene Structure 3214 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7AG428100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3126 bp

ATGTGTCGTACAAACACTCCGAAAGAGAAAATCACAATGGACAAAATTGTAATCATTGACCCTCATCCTCAACAAGCAGCCTTGTGGTTAAAATGGCACACTCATGGAGTCTCACTTACGCATTCCCATCTTCAAAGTCCATTCACCCACTCTTCGTCTGTCAACTCCATCATGCCATTACCCAAAAACCACCACCTCCATCTCTACCTCCTCTTCTTCCTCTTCCTCCTCTCCAACGCCGCCGCAGCCGACGAGCTTCAAATCCTACTAAAACTCAAATCCTCCCTCCAGGACTCAAACTCCCAACTCTTCGCCACATGGGACTCCACCGCCAATTCGTTTTGCAAATTCACCGGAATCACCTGCAACGACGTCGGTTCGGTTCGAGAAATCGAACTTTCGAACCAGAAGCTGTCGGGGTCTCTTCCGCTGGACTCCATATGCCAACTTCCCTCATTAGAAAAGCTCGCTTTCGGGTCCAATTTCCTGCACGGTACAATCACGGAGGACTTGAGAAACTGTACGAAGCTGAAATACCTGGATTTGGGGAAAAACTTGTTCGCGGGTTCATTCCCTGACATATCTTTCTTGTCCCAACTGGAGCATCTTAATCTGAACGGCAGCTGGTTTTCCGGGATTTTCCCGTGGACTTCCCTCACCAACATGACTGGTCTGATTCGTTTGAGCTTGGGTGACAACCCGTTTGATCCGAGCCCGTTTCCAAAGGAAGTTGTAAATCTTAACAAGCTCGAGTGGCTCTACTTGACAAACTGCAGCATCCAAGGAACAATACCAAGTGAAATCGGGAACTTGGTCGAGCTTATCAACCTGGAGTTGTCCGATAACAACATGACCGGAGAAATTCCGGCCGAGATTGGACAACTCACCAAGCTCTGGCAGCTGGAGCTCTACACCAACAGGTTCACCGGAAAGCTTCCTTTCGGGCTAAGAAACCTCACCATTCTCGAGAACTTCGACGCCTCTGAGAATTTTCTGGAAGGCGATTTGAACGAGTTGAGGTTTCTGACCAACTTGGTTTCTCTGCAGCTGTATGAGAACAATTTTTCGGGCGAATTACCGGAAGAGTTCGGCGAATTAAAGAAGCTTGTGAATCTGTCTTTGTACGGTAACAAGCTGACCGGTCCTCTGCCTCAGAAATTGGGGTCTTGGTCGGAGATGGACTTCATCGACGTGTCGGAGAACTTCTTGACCGGGACTATTCCGCCGGACATGTGCAACAAAGGGACGATGAAGCAGCTGCTCATGCTTCAGAACCAATTAACTGGCGAAATTCCGGCAAACTACGCCAAGTGCACGACGTTGACCCGGTTCAGGGTCAACAACAACTCCCTCTCCGGTGTGGTTCCGGCAGGACTTTGGGGATTGCCGAATGTGGCAATCATTGACATCACTTCGAATCAAATTGAAGGGCCGATTACTTCCGATATCGGAAACGCCAAGAAGCTTGCGCAGTTGTTTGTGAGTTACAATCGGTTATCTGGTGAATTACCAGACGAGCTTTCGAAGTCAACTTCGTTGGTTTCGGTTGTGTTGAATAACAATCAGTTTTCCGGTAAGATTCCGGCGAAGATAGGGGACTTGAAGCAATTGGGTACTCTGTATTTACAGAGCAACTTGTTGTCTTCTTCGATTCCCAAGTCATTAGGAAGCTGTAGTTTCCTGAATGACTTGAACATGGCAAACAACTCGCTTTCCGGTGAAATCCCAGCATCTTTGGGCTCTTTGCCGACCTTGAATTCTCTGGATTTGTCTCACAATCTACTCTCCGGTAAAATCCCGGAAAGTCTGGCATCTCTGAGACTAAGCATGCTTGATCTTTCACACAACAGACTGACCGGTGCCGTACCGGAATCTCTCTCGATTGCAGCCTACAACGGCAGCCTTTCCGGTAACCCGGGTCTCTGCAGCATGGACATCACCTACTTCCCACGGTGCTCGCCGAAAAAGGAAATGTCCGACGATGTCAGGACACTCATTATTTGCTTCTCAGTGGGTACAGCAATATTGTTTGTTTCACTCATCTGCTTCTTGTTCTTAAAGAGGAAGGAGAAAGATCAAGACCGTTCATTGAAGGAAGAATCTTGGGATGTAAAGTCTTTCCATGTAATAACCTTCAGTGAGGATGAGATTCTTGATTCCATCACACAAGAGAATCTAATTGGAAAAGGAGGTTCTGGAAATGTCTACAAAGTGTCACTAACCAATGGCAAAGAACTCGCCGTGAAGCACATATGGAACACTGATCCAAGCGGCCGGAAAATATTCAAGAGCACCACACCGATGCTCGGGAGACGGGACAGTGGTAGTTCGAAATCGAAGGAATTTGATGCTGAGGTGCAGACGTTGAGCTCAATAAGGCATGTGAATGTGGTGAAGTTGTTCTGCAGCATTACTAGTGAGGACTCGAGCCTTCTGGTCTACGAGTATTTACCGAATGGAAGCTTGTGGGATCGGCTGCACATGTGTGAGAAGATGAAGCTTGATTGGGACGCAAGGTATGAGATTGCAGTCGGAGCAGCCAAAGGGTTGGAGTATCTGCACCATGGCTGTGAGAGGCTAGTAATTCATAGAGATGTCAAGTCCAGTAACATTTTGTTAGACGAGTTTTTGAAGCCCCGGATTGCGGATTTTGGGCTCGCCAAGATGGTTCAGACTAATGCAATCAAGGACTCAACTCATGTTGTTGCTGGAACACACGGTTACATAGCTCCTGAATATGGTTACACCTACAAGGTGAATGAGAAGAGCGATGTGTATAGCTTCGGTGTAGTACTAATGGAGCTAGTGACTGGGAAAAAGCCGATAGATCCATCATTTGGGGACAACAAGGACATAGTGAACTGGATATGCGGCAACCTGAAGAGTAGAGAGTGTGTGTTAGGTGTGGTGGACTCATACATTCCTGAGGCATATAGAGAAGAGGCTATCAAGGTATTGAGAATTGCAATTCTATGCACAGCTAGGCTTCCAGAGCTAAGACCTTCCATGAGAAGTGTCGTTCAAATGCTTGAAGAAGCTCACGAGCCAATGAAATTGCTGGACATTGTTATCAGCAAAGATGGTTCTAGTAAGAAAATGGAAGTACTTAAGGGAACAGAGAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001653 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009791 GO:0009845 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045087 GO:0046777 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0090351 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

1041

Amino Acids

115.51

Weight (kDa)

5.92

Isoelectric Point (pI)

31.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	83 - 123	2.8e-09	Leucine rich repeat N-terminal domain
LRR_14	PF23598	242 - 350	7.3e-12	Leucine-rich repeat region
Pkinase	PF00069	723 - 1008	8.1e-40	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	723 - 1010	2.8e-37	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004091)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09970 AT1G09970
fragaria_vesca	FvH4_5g33590
malus_domestica	MD08G1210000.v1.1 MD15G1394100.v1.1
prunus_persica	Prupe.1G542300_v2.0.a1
pyrus_communis	pycom08g18120 pycom15g35400 pycom15g35410
rosa_chinensis	RchiOBHm_Chr7g0217331 RchiOBHm_Chr7g0232471
rosa_laevigata	RLG00000001335 RLG00000002489 RLG00000002493
rosa_multiflora	Rmu_sc0001898.1_g000015 Rmu_sc0004663.1_g000011 Rmu_sc0011824.1_g000016
rosa_roxburghii	Rroxscaffold_3G00228280 Rroxscaffold_3G00242430
rosa_rugosa	Rorug07G0168100 Rorug07G0274700
rosa_samantha	Rh7AG307400 Rh7AG307800 Rh7AG428100 Rh7BG298200 Rh7BG402600 Rh7CG326500 Rh7CG326900 Rh7CG447100 Rh7DG306900 Rh7DG418900
rosa_wichuraiana	Rw7G026090 Rw7G035470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	371
AatII	GACGTC	1 cut(s)	375
Acc16I	TGCGCA	1 cut(s)	1470
Acc36I	ACCTGC	1 cut(s)	371
AccB1I	GGYRCC	1 cut(s)	1859
AccB7I	CCANNNNNTGG	2 cut(s)	894, 2837
AccI	GTMKAC	2 cut(s)	2205, 2439
AccII	CGCG	1 cut(s)	563
AciI	CCGC	9 cut(s)	240, 243, 330, 428, 563, 1223, 2271, 2645, 2871
AclWI	GGATC	4 cut(s)	701, 2258, 2478, 2822
AcoI	YGGCCR	2 cut(s)	867, 2272
AcsI	RAATTY	6 cut(s)	347, 861, 988, 1290, 1756, 2342
AcuI	CTGAAG	3 cut(s)	1253, 2119, 2900
AcyI	GRCGYC	2 cut(s)	372, 978
AfaI	GTAC	9 cut(s)	10, 496, 523, 1124, 1615, 1866, 2017, 2793, 3105
AfiI	CCNNNNNNNGG	8 cut(s)	544, 894, 1332, 1333, 2306, 2644, 2738, 2837
AflII	CTTAAG	1 cut(s)	3107
AflIII	ACRYGT	3 cut(s)	1191, 1230, 2481
AgeI	ACCGGT	2 cut(s)	1139, 1856
AhlI	ACTAGT	1 cut(s)	2414
AjiI	CACGTC	1 cut(s)	1192
AjnI	CCWGG	3 cut(s)	288, 537, 828
AleI	CACNNNNGTG	1 cut(s)	2484
Alw21I	GWGCWC	5 cut(s)	909, 1316, 1958, 2294, 2372
Alw26I	GTCTC	6 cut(s)	115, 426, 1776, 1813, 1925, 2305
Alw44I	GTGCAC	1 cut(s)	1312
AlwI	GGATC	4 cut(s)	701, 2258, 2478, 2822
AlwNI	CAGNNNCTG	1 cut(s)	412
Ama87I	CYCGRG	5 cut(s)	749, 965, 1915, 2306, 2425
AoxI	GGCC	3 cut(s)	867, 1430, 2272
ApaLI	GTGCAC	1 cut(s)	1312
ApoI	RAATTY	6 cut(s)	347, 861, 988, 1290, 1756, 2342
ArsI	GACNNNNNNTTYG	2 cut(s)	686, 718
AsiGI	ACCGGT	2 cut(s)	1139, 1856
Asp700I	GAANNNNTTC	2 cut(s)	2282, 2399
AspLEI	GCGC	1 cut(s)	1471
AspS9I	GGNCC	3 cut(s)	477, 1142, 1430
AsuC2I	CCSGG	7 cut(s)	634, 1213, 1327, 1799, 1916, 1917, 2638
AsuII	TTCGAA	4 cut(s)	401, 1415, 1523, 2330
AvaI	CYCGRG	5 cut(s)	749, 965, 1915, 2306, 2425
AvaII	GGWCC	2 cut(s)	477, 1142
AxyI	CCTNAGG	1 cut(s)	2925
BaeGI	GKGCMC	1 cut(s)	1316
BanI	GGYRCC	1 cut(s)	1859
BanII	GRGCYC	5 cut(s)	716, 909, 1742, 2372, 2658
BarI	GAAGNNNNNNTAC	2 cut(s)	518, 550
BauI	CACGAG	1 cut(s)	3041
BbsI	GAAGAC	1 cut(s)	1635
Bbv12I	GWGCWC	5 cut(s)	909, 1316, 1958, 2294, 2372
BbvCI	CCTCAGC	1 cut(s)	2352
BccI	CCATC	8 cut(s)	135, 176, 207, 1171, 2168, 2659, 2839, 3074
BceAI	ACGGC	4 cut(s)	634, 1847, 1912, 2225
BcgI	CGANNNNNNTGC	6 cut(s)	355, 389, 1689, 1723, 2218, 2252
BciT130I	CCWGG	3 cut(s)	290, 539, 830
BcnI	CCSGG	7 cut(s)	634, 1213, 1327, 1799, 1916, 1917, 2638
BcoDI	GTCTC	6 cut(s)	115, 426, 1776, 1813, 1925, 2305
BcuI	ACTAGT	1 cut(s)	2414
BfaI	CTAG	5 cut(s)	2415, 2579, 2804, 2982, 3087
BfmI	CTRYAG	5 cut(s)	772, 1043, 1672, 1924, 2404
BfrI	CTTAAG	1 cut(s)	3107
BfuAI	ACCTGC	1 cut(s)	371
BmcAI	AGTACT	2 cut(s)	2793, 3105
Bme18I	GGWCC	2 cut(s)	477, 1142
BmeT110I	CYCGRG	5 cut(s)	749, 965, 1915, 2306, 2425
BmgBI	CACGTC	1 cut(s)	1192
BmgT120I	GGNCC	3 cut(s)	477, 1142, 1430
BmiI	GGNNCC	3 cut(s)	478, 1365, 1861
BmrI	ACTGGG	1 cut(s)	2820
BmsI	GCATC	6 cut(s)	613, 786, 1739, 1820, 2292, 2338
BmuI	ACTGGG	1 cut(s)	2820
BpiI	GAAGAC	1 cut(s)	1635
BpmI	CTGGAG	4 cut(s)	272, 620, 851, 923
Bpu10I	CCTNAGC	1 cut(s)	2352
Bpu14I	TTCGAA	4 cut(s)	401, 1415, 1523, 2330
BpuEI	CTTGAG	1 cut(s)	532
BpuMI	CCSGG	7 cut(s)	634, 1213, 1327, 1799, 1916, 1917, 2638
BsaHI	GRCGYC	2 cut(s)	372, 978
BsaI	GGTCTC	2 cut(s)	426, 1925
BsaJI	CCNNGG	6 cut(s)	644, 781, 1915, 1949, 2563, 2636
BsaXI	ACNNNNNCTCC	7 cut(s)	31, 284, 314, 1331, 1337, 1361, 1367
Bsc4I	CCNNNNNNNGG	8 cut(s)	544, 894, 1332, 1333, 2306, 2644, 2738, 2837
Bse118I	RCCGGY	2 cut(s)	1139, 1856
Bse1I	ACTGG	6 cut(s)	603, 673, 1288, 2604, 2815, 2867
Bse21I	CCTNAGG	1 cut(s)	2925
BseBI	CCWGG	3 cut(s)	290, 539, 830
BseDI	CCNNGG	6 cut(s)	644, 781, 1915, 1949, 2563, 2636
BseGI	GGATG	4 cut(s)	64, 777, 2113, 2149
BseLI	CCNNNNNNNGG	8 cut(s)	544, 894, 1332, 1333, 2306, 2644, 2738, 2837
BseMII	CTCAG	6 cut(s)	975, 1166, 1808, 2022, 2343, 2916
BseNI	ACTGG	6 cut(s)	603, 673, 1288, 2604, 2815, 2867
BseRI	GAGGAG	2 cut(s)	200, 218
BseSI	GKGCMC	1 cut(s)	1316
BseX3I	CGGCCG	2 cut(s)	867, 2272
BseYI	CCCAGC	1 cut(s)	1726
BsgI	GTGCAG	4 cut(s)	473, 2378, 2462, 2543
Bsh1236I	CGCG	1 cut(s)	563
Bsh1285I	CGRYCG	2 cut(s)	870, 2275
BshFI	GGCC	3 cut(s)	869, 1432, 2274
BshNI	GGYRCC	1 cut(s)	1859
BshTI	ACCGGT	2 cut(s)	1139, 1856
BsiEI	CGRYCG	2 cut(s)	870, 2275
BsiHKAI	GWGCWC	5 cut(s)	909, 1316, 1958, 2294, 2372
BsiHKCI	CYCGRG	5 cut(s)	749, 965, 1915, 2306, 2425
BslFI	GGGAC	8 cut(s)	335, 577, 1228, 1258, 1610, 2330, 2522, 2855
BslI	CCNNNNNNNGG	8 cut(s)	544, 894, 1332, 1333, 2306, 2644, 2738, 2837
BsmAI	GTCTC	6 cut(s)	115, 426, 1776, 1813, 1925, 2305
BsmBI	CGTCTC	1 cut(s)	2305
BsmFI	GGGAC	8 cut(s)	335, 577, 1228, 1258, 1610, 2330, 2522, 2855
BsmI	GAATGC	1 cut(s)	121
BsnI	GGCC	3 cut(s)	869, 1432, 2274
Bso31I	GGTCTC	2 cut(s)	426, 1925
BsoBI	CYCGRG	5 cut(s)	749, 965, 1915, 2306, 2425
Bsp119I	TTCGAA	4 cut(s)	401, 1415, 1523, 2330
Bsp1286I	GDGCHC	8 cut(s)	716, 909, 1316, 1742, 1958, 2294, 2372, 2658
Bsp143I	GATC	6 cut(s)	706, 1834, 2074, 2263, 2470, 2827
Bsp19I	CCATGG	1 cut(s)	2563
BspACI	CCGC	9 cut(s)	240, 243, 330, 428, 563, 1223, 2271, 2645, 2871
BspANI	GGCC	3 cut(s)	869, 1432, 2274
BspCNI	CTCAG	6 cut(s)	976, 1165, 1809, 2021, 2344, 2917
BspFNI	CGCG	1 cut(s)	563
BspLI	GGNNCC	3 cut(s)	478, 1365, 1861
BspMAI	CTGCAG	4 cut(s)	776, 1047, 1928, 2408
BspMI	ACCTGC	1 cut(s)	371
BspPI	GGATC	4 cut(s)	701, 2258, 2478, 2822
BspQI	GCTCTTC	1 cut(s)	2759
BspT104I	TTCGAA	4 cut(s)	401, 1415, 1523, 2330
BspT107I	GGYRCC	1 cut(s)	1859
BspTI	CTTAAG	1 cut(s)	3107
BspTNI	GGTCTC	2 cut(s)	426, 1925
BsrFI	RCCGGY	2 cut(s)	1139, 1856
BsrI	ACTGG	6 cut(s)	603, 673, 1288, 2604, 2815, 2867
BssAI	RCCGGY	2 cut(s)	1139, 1856
BssECI	CCNNGG	6 cut(s)	644, 781, 1915, 1949, 2563, 2636
BssMI	GATC	6 cut(s)	706, 1834, 2074, 2263, 2470, 2827
BssNI	GRCGYC	2 cut(s)	372, 978
BssSI	CACGAG	1 cut(s)	3041
BssT1I	CCWWGG	2 cut(s)	781, 2563
Bst2BI	CACGAG	1 cut(s)	3041
Bst2UI	CCWGG	3 cut(s)	290, 539, 830
Bst4CI	ACNGT	7 cut(s)	494, 521, 1127, 1953, 2084, 2321, 2720
Bst6I	CTCTTC	9 cut(s)	157, 218, 227, 314, 429, 1077, 2759, 2876, 2934
BstACI	GRCGYC	2 cut(s)	372, 978
BstAFI	CTTAAG	1 cut(s)	3107
BstBI	TTCGAA	4 cut(s)	401, 1415, 1523, 2330
BstC8I	GCNNGC	5 cut(s)	468, 1467, 1829, 1958, 2658
BstDEI	CTNAG	9 cut(s)	947, 984, 1152, 1817, 1823, 2008, 2352, 2925, 2996
BstDSI	CCRYGG	3 cut(s)	644, 1949, 2563
BstEII	GGTNACC	1 cut(s)	1910
BstF5I	GGATG	4 cut(s)	64, 777, 2113, 2149
BstFNI	CGCG	1 cut(s)	563
BstHHI	GCGC	1 cut(s)	1471
BstKTI	GATC	6 cut(s)	709, 1837, 2077, 2266, 2473, 2830
BstMAI	GTCTC	6 cut(s)	115, 426, 1776, 1813, 1925, 2305
BstMBI	GATC	6 cut(s)	706, 1834, 2074, 2263, 2470, 2827
BstMCI	CGRYCG	2 cut(s)	870, 2275
BstMWI	GCNNNNNNNGC	8 cut(s)	245, 754, 898, 904, 1264, 1304, 2534, 2992
BstNI	CCWGG	3 cut(s)	290, 539, 830
BstNSI	RCATGY	4 cut(s)	1234, 1831, 2384, 2485
BstPI	GGTNACC	1 cut(s)	1910
BstSFI	CTRYAG	5 cut(s)	772, 1043, 1672, 1924, 2404
BstSLI	GKGCMC	1 cut(s)	1316
BstUI	CGCG	1 cut(s)	563
BstV2I	GAAGAC	1 cut(s)	1635
BstX2I	RGATCY	1 cut(s)	2827
BstXI	CCANNNNNNTGG	2 cut(s)	1283, 2548
BstYI	RGATCY	1 cut(s)	2827
BstZI	CGGCCG	2 cut(s)	867, 2272
Bsu36I	CCTNAGG	1 cut(s)	2925
BsuRI	GGCC	3 cut(s)	869, 1432, 2274
BtgI	CCRYGG	3 cut(s)	644, 1949, 2563
BtgZI	GCGATG	1 cut(s)	2784
BtrI	CACGTC	1 cut(s)	1192
BtsCI	GGATG	4 cut(s)	64, 777, 2113, 2149
BtsIMutI	CAGTG	4 cut(s)	2016, 2143, 2259, 2326
BveI	ACCTGC	1 cut(s)	371
Cac8I	GCNNGC	5 cut(s)	468, 1467, 1829, 1958, 2658
CaiI	CAGNNNCTG	1 cut(s)	412
CfoI	GCGC	1 cut(s)	1471
Cfr10I	RCCGGY	2 cut(s)	1139, 1856
Cfr13I	GGNCC	3 cut(s)	477, 1142, 1430
Cfr9I	CCCGGG	1 cut(s)	1915
CseI	GACGC	2 cut(s)	986, 2519
Csp6I	GTAC	9 cut(s)	9, 495, 522, 1123, 1614, 1865, 2016, 2792, 3104
CspAI	ACCGGT	2 cut(s)	1139, 1856
CviQI	GTAC	9 cut(s)	9, 495, 522, 1123, 1614, 1865, 2016, 2792, 3104
DdeI	CTNAG	9 cut(s)	947, 984, 1152, 1817, 1823, 2008, 2352, 2925, 2996
DpnI	GATC	6 cut(s)	708, 1836, 2076, 2265, 2472, 2829
DpnII	GATC	6 cut(s)	706, 1834, 2074, 2263, 2470, 2827
EaeI	YGGCCR	2 cut(s)	867, 2272
EagI	CGGCCG	2 cut(s)	867, 2272
Eam1104I	CTCTTC	9 cut(s)	157, 218, 227, 314, 429, 1077, 2759, 2876, 2934
EarI	CTCTTC	9 cut(s)	157, 218, 227, 314, 429, 1077, 2759, 2876, 2934
EciI	GGCGGA	1 cut(s)	1212
Ecl136II	GAGCTC	2 cut(s)	907, 2370
EclXI	CGGCCG	2 cut(s)	867, 2272
Eco130I	CCWWGG	2 cut(s)	781, 2563
Eco24I	GRGCYC	5 cut(s)	716, 909, 1742, 2372, 2658
Eco31I	GGTCTC	2 cut(s)	426, 1925
Eco32I	GATATC	1 cut(s)	1447
Eco47I	GGWCC	2 cut(s)	477, 1142
Eco52I	CGGCCG	2 cut(s)	867, 2272
Eco53kI	GAGCTC	2 cut(s)	907, 2370
Eco57I	CTGAAG	3 cut(s)	1253, 2119, 2900
Eco81I	CCTNAGG	1 cut(s)	2925
Eco88I	CYCGRG	5 cut(s)	749, 965, 1915, 2306, 2425
Eco91I	GGTNACC	1 cut(s)	1910
EcoICRI	GAGCTC	2 cut(s)	907, 2370
EcoO65I	GGTNACC	1 cut(s)	1910
EcoRI	GAATTC	1 cut(s)	1756
EcoRII	CCWGG	3 cut(s)	288, 537, 828
EcoRV	GATATC	1 cut(s)	1447
EcoT14I	CCWWGG	2 cut(s)	781, 2563
EcoT38I	GRGCYC	5 cut(s)	716, 909, 1742, 2372, 2658
ErhI	CCWWGG	2 cut(s)	781, 2563
Esp3I	CGTCTC	1 cut(s)	2305
FaqI	GGGAC	8 cut(s)	335, 577, 1228, 1258, 1610, 2330, 2522, 2855
FauI	CCCGC	1 cut(s)	556
FauNDI	CATATG	2 cut(s)	440, 2252
FblI	GTMKAC	2 cut(s)	2205, 2439
FokI	GGATG	4 cut(s)	51, 764, 2120, 2156
FriOI	GRGCYC	5 cut(s)	716, 909, 1742, 2372, 2658
FspBI	CTAG	5 cut(s)	2415, 2579, 2804, 2982, 3087
FspI	TGCGCA	1 cut(s)	1470
GlaI	GCGC	1 cut(s)	1470
GsaI	CCCAGC	1 cut(s)	1730
GsuI	CTGGAG	4 cut(s)	272, 620, 851, 923
HaeIII	GGCC	3 cut(s)	869, 1432, 2274
HgaI	GACGC	2 cut(s)	986, 2519
HhaI	GCGC	1 cut(s)	1471
Hin1I	GRCGYC	2 cut(s)	372, 978
Hin6I	GCGC	1 cut(s)	1469
HinP1I	GCGC	1 cut(s)	1469
HincII	GTYRAC	4 cut(s)	163, 1323, 1339, 1530
HindII	GTYRAC	4 cut(s)	163, 1323, 1339, 1530
HindIII	AAGCTT	5 cut(s)	932, 1103, 1463, 2461, 2498
Hpy99I	CGWCG	7 cut(s)	254, 374, 377, 980, 1193, 1321, 1982
HpyAV	CCTTC	8 cut(s)	992, 1421, 2059, 2086, 2143, 2332, 2441, 3012
HpyCH4III	ACNGT	7 cut(s)	494, 521, 1127, 1953, 2084, 2321, 2720
HpyCH4IV	ACGT	4 cut(s)	372, 1191, 1319, 2363
HpyF10VI	GCNNNNNNNGC	8 cut(s)	245, 754, 898, 904, 1264, 1304, 2534, 2992
HpyF3I	CTNAG	9 cut(s)	947, 984, 1152, 1817, 1823, 2008, 2352, 2925, 2996
HpySE526I	ACGT	4 cut(s)	372, 1191, 1319, 2363
Hsp92I	GRCGYC	2 cut(s)	372, 978
HspAI	GCGC	1 cut(s)	1469
Kzo9I	GATC	6 cut(s)	706, 1834, 2074, 2263, 2470, 2827
LguI	GCTCTTC	1 cut(s)	2759
LmnI	GCTCC	5 cut(s)	601, 904, 2534, 2734, 2800
LweI	GCATC	6 cut(s)	613, 786, 1739, 1820, 2292, 2338
MaeI	CTAG	5 cut(s)	2415, 2579, 2804, 2982, 3087
MaeII	ACGT	4 cut(s)	372, 1191, 1319, 2363
MaeIII	GTNAC	9 cut(s)	692, 1127, 1484, 1910, 2214, 2606, 2720, 2741, 2806
MalI	GATC	6 cut(s)	708, 1836, 2076, 2265, 2472, 2829
MboI	GATC	6 cut(s)	706, 1834, 2074, 2263, 2470, 2827
MfeI	CAATTG	1 cut(s)	1607
MflI	RGATCY	1 cut(s)	2827
MhlI	GDGCHC	8 cut(s)	716, 909, 1316, 1742, 1958, 2294, 2372, 2658
MlyI	GAGTC	7 cut(s)	117, 287, 317, 428, 2417, 2684, 2906
MmeI	TCCRAC	6 cut(s)	258, 1152, 1176, 2001, 2512, 2529
MroXI	GAANNNNTTC	2 cut(s)	2282, 2399
MseI	TTAA	7 cut(s)	89, 609, 741, 1097, 1280, 2057, 3108
MslI	CAYNNNNRTG	8 cut(s)	102, 662, 1190, 2300, 2385, 2484, 2568, 2592
MspA1I	CMGCKG	5 cut(s)	430, 624, 901, 1048, 1258
MspCI	CTTAAG	1 cut(s)	3107
MunI	CAATTG	1 cut(s)	1607
Mva1269I	GAATGC	1 cut(s)	121
MvaI	CCWGG	3 cut(s)	290, 539, 830
MvnI	CGCG	1 cut(s)	563
MwoI	GCNNNNNNNGC	8 cut(s)	245, 754, 898, 904, 1264, 1304, 2534, 2992
NciI	CCSGG	7 cut(s)	634, 1213, 1327, 1799, 1916, 1917, 2638
NcoI	CCATGG	1 cut(s)	2563
NdeI	CATATG	2 cut(s)	440, 2252
NdeII	GATC	6 cut(s)	706, 1834, 2074, 2263, 2470, 2827
NlaIV	GGNNCC	3 cut(s)	478, 1365, 1861
NmeAIII	GCCGAG	1 cut(s)	895
NmuCI	GTSAC	3 cut(s)	692, 2214, 2806
NsbI	TGCGCA	1 cut(s)	1470
NspI	RCATGY	4 cut(s)	1234, 1831, 2384, 2485
NspV	TTCGAA	4 cut(s)	401, 1415, 1523, 2330
OliI	CACNNNNGTG	1 cut(s)	2484
PaeI	GCATGC	1 cut(s)	1831
PaeR7I	CTCGAG	3 cut(s)	749, 965, 2425
PaqCI	CACCTGC	1 cut(s)	371
PciI	ACATGT	2 cut(s)	1230, 2481
PciSI	GCTCTTC	1 cut(s)	2759
PcsI	WCGNNNNNNNCGW	1 cut(s)	972
PctI	GAATGC	1 cut(s)	121
PdmI	GAANNNNTTC	2 cut(s)	2282, 2399
PflFI	GACNNNGTC	1 cut(s)	1982
PflMI	CCANNNNNTGG	2 cut(s)	894, 2837
PfoI	TCCNGGA	3 cut(s)	288, 632, 1797
PinAI	ACCGGT	2 cut(s)	1139, 1856
PleI	GAGTC	7 cut(s)	116, 287, 317, 428, 2417, 2684, 2906
PpsI	GAGTC	7 cut(s)	116, 287, 317, 428, 2417, 2684, 2906
PscI	ACATGT	2 cut(s)	1230, 2481
Psp124BI	GAGCTC	2 cut(s)	909, 2372
Psp6I	CCWGG	3 cut(s)	288, 537, 828
PspEI	GGTNACC	1 cut(s)	1910
PspFI	CCCAGC	1 cut(s)	1726
PspGI	CCWGG	3 cut(s)	288, 537, 828
PspN4I	GGNNCC	3 cut(s)	478, 1365, 1861
PspPI	GGNCC	3 cut(s)	477, 1142, 1430
PspXI	VCTCGAGB	2 cut(s)	749, 2425
PstI	CTGCAG	4 cut(s)	776, 1047, 1928, 2408
PstNI	CAGNNNCTG	1 cut(s)	412
PsuI	RGATCY	1 cut(s)	2827
PsyI	GACNNNGTC	1 cut(s)	1982
PvuII	CAGCTG	4 cut(s)	624, 901, 1048, 1258
RsaI	GTAC	9 cut(s)	10, 496, 523, 1124, 1615, 1866, 2017, 2793, 3105
RsaNI	GTAC	9 cut(s)	9, 495, 522, 1123, 1614, 1865, 2016, 2792, 3104
RseI	CAYNNNNRTG	8 cut(s)	102, 662, 1190, 2300, 2385, 2484, 2568, 2592
SacI	GAGCTC	2 cut(s)	909, 2372
SapI	GCTCTTC	1 cut(s)	2759
SaqAI	TTAA	7 cut(s)	89, 609, 741, 1097, 1280, 2057, 3108
Sau3AI	GATC	6 cut(s)	706, 1834, 2074, 2263, 2470, 2827
Sau96I	GGNCC	3 cut(s)	477, 1142, 1430
ScaI	AGTACT	2 cut(s)	2793, 3105
SchI	GAGTC	7 cut(s)	117, 287, 317, 428, 2417, 2684, 2906
SduI	GDGCHC	8 cut(s)	716, 909, 1316, 1742, 1958, 2294, 2372, 2658
SfaNI	GCATC	6 cut(s)	613, 786, 1739, 1820, 2292, 2338
SfcI	CTRYAG	5 cut(s)	772, 1043, 1672, 1924, 2404
Sfr274I	CTCGAG	3 cut(s)	749, 965, 2425
SfuI	TTCGAA	4 cut(s)	401, 1415, 1523, 2330
SinI	GGWCC	2 cut(s)	477, 1142
SlaI	CTCGAG	3 cut(s)	749, 965, 2425
SmaI	CCCGGG	1 cut(s)	1917
SmiMI	CAYNNNNRTG	8 cut(s)	102, 662, 1190, 2300, 2385, 2484, 2568, 2592
SmlI	CTYRAG	5 cut(s)	511, 749, 965, 2425, 3107
SmoI	CTYRAG	5 cut(s)	511, 749, 965, 2425, 3107
SpeI	ACTAGT	1 cut(s)	2414
SphI	GCATGC	1 cut(s)	1831
SsiI	CCGC	9 cut(s)	240, 243, 330, 428, 563, 1223, 2271, 2645, 2871
SspI	AATATT	2 cut(s)	2025, 2283
SspMI	CTAG	5 cut(s)	2415, 2579, 2804, 2982, 3087
SstI	GAGCTC	2 cut(s)	909, 2372
StyI	CCWWGG	2 cut(s)	781, 2563
TaaI	ACNGT	7 cut(s)	494, 521, 1127, 1953, 2084, 2321, 2720
TaiI	ACGT	4 cut(s)	375, 1194, 1322, 2366
TatI	WGTACW	2 cut(s)	2791, 3103
TauI	GCSGC	4 cut(s)	242, 245, 2274, 2874
Tru1I	TTAA	7 cut(s)	89, 609, 741, 1097, 1280, 2057, 3108
Tru9I	TTAA	7 cut(s)	89, 609, 741, 1097, 1280, 2057, 3108
TscAI	CASTG	4 cut(s)	2016, 2143, 2266, 2326
TseFI	GTSAC	3 cut(s)	692, 2214, 2806
Tsp45I	GTSAC	3 cut(s)	692, 2214, 2806
TspDTI	ATGAA	7 cut(s)	558, 1174, 1265, 2076, 2510, 2576, 3065
TspGWI	ACGGA	1 cut(s)	518
TspMI	CCCGGG	1 cut(s)	1915
TspRI	CASTG	4 cut(s)	2016, 2143, 2266, 2326
Tth111I	GACNNNGTC	1 cut(s)	1982
Van91I	CCANNNNNTGG	2 cut(s)	894, 2837
Vha464I	CTTAAG	1 cut(s)	3107
VneI	GTGCAC	1 cut(s)	1312
VpaK11BI	GGWCC	2 cut(s)	477, 1142
XapI	RAATTY	6 cut(s)	347, 861, 988, 1290, 1756, 2342
XceI	RCATGY	4 cut(s)	1234, 1831, 2384, 2485
XhoI	CTCGAG	3 cut(s)	749, 965, 2425
XmaI	CCCGGG	1 cut(s)	1915
XmiI	GTMKAC	2 cut(s)	2205, 2439
XmnI	GAANNNNTTC	2 cut(s)	2282, 2399
XspI	CTAG	5 cut(s)	2415, 2579, 2804, 2982, 3087
ZraI	GACGTC	1 cut(s)	373
ZrmI	AGTACT	2 cut(s)	2793, 3105

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.