Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0004091

Physical Location & Seq

Genomic Coordinates Forward (+)

49157647 .. 49161381

Gene Structure 3735 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1394100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2961 bp

ATGTCGTCGTCGACAGGCCGGAGAAAATTCCATCTACCCCTCCACATCCTCTTCCTCCTCCTTTGCCTCCTCTCCGCCGCGGCGGCCGACGAGCTTCAGATTCTTCTCACACTCAAATCCGCGTTCCAAGGTTCAAACACCAACAACATATTCAACACATGGAACTCCACCAGTCGCGTTTGCAGCTTCACCGGAATTGCCTGCAACGAAAACGGATTCGTTCGAGATATCGATCTTTCGAATCAGAACCTATCCGGGTTTCTTGCGGCGGACGCAATATGCCAGCTCAAATCATTGGAAACGCTTTCCTTGGGGTTCAACTTCTTGGACGGGACAATCAAAGAGGACCTGAACAACTGCGTAAATTTGAAGTACTTGGACTTGGGCCACAACTCGTTCTCAGGATCGTTCCCCGACATATCATCCCTGTCCCGGTTAGAACATCTTCATCTCAACAACAGCGGATTTTCGGGTACTTTTCCATGGAAATCATTGGCGAACATGACGGGTTTGATTCTACTGAGCTTGGGAGACAACCCTTTTGATCAAAGCCTATTCCCAACCGAGTTGTTGAATCTGAAGAAACTCAACTGGCTTTACTTGGCGAATTGCAGCCTCGGAGGTCCGATCCCGAAGGGAATCGGAAACCTCACGGAGCTTATCAACTTGGAGTTATCCCAAAACAATATGGTCGGAGAAATCCCATCCGAGATTACAAAGCTCAACAAGCTCTGGCAGCTCGAGCTCTACGACAACAAGCTCACCGGGAGGCTTCCTTCCGGCCTAGGAAACCTCACCAACCTCGAAAACTTCGACGCCTCCGCGAATCGACTCGAAGGCGATTTGTCGGAGCTGAGATTTTTGGAGAACTCAGTTACCCTCCAATTCTACGAGAACAATTTCACCGGAGAAATACCTGCCGAGTTCGGGGAATTCAAGAAGCTTGTCAATCTGTCCTTGTACACGAACAAGCTGACCGGTCCTCTGCCTCAGAAACTTGGCTCTTGGTCCAAGACTGATTTCATTGATGTCTCTGAGAACCTCCTGACGGGGAGTATTCCGCCGGATATGTGCAAGATGGGGACGATGAGAGGCTTTTTCTTGCTCCAGAACAACTTTACCGGCGAAATTCCGGCGAATTACGCAAAATGTCCGACGTTGAAGCGGTTCAGAGTTAACATCAACTCGCTGTCCGGTGTTGTTCCTCCTGGAATTTGGGGGTTGCCGAATGCGGAAATCATCGACCTTACTTCCAATCAATTCCAAGGCCCGATTACTTCTGACATCGGAAACGCGAAGACGCTTGCGCAGTTGTTCGTCAGTTACAATCAGTTATCTGGCGAGCTGCCGGATGAGATTTCCAAAGCAACATCTTTGTTGTCGATTGTTTTGAACAACAATAGGTTTTCGGGGAAAATCCCGGGGAATATTGGTGACTTGAAGCATCTGGGGACTCTGTATTTGCAGAGCAACATGTTCTCTGCTTCGATACCAAAGTCTTTAGGAAGCTGTGATTTTCTGAGTGACTTAAACATTGCTGAAAACTCGCTTTCCGGTGATATCCCATCATCGTTAGGCTCTCTTCCAACCTTGAACTCTCTGAATTTGTCGCGAAATCAACTTTCGGGTGAAATCCCGAAGAGCTTAGGATCTCTAAGGCTAAGCCTTCTTGACCTATCAGACAACAGGCTCACCGGCGCCATACCAAAATCTCTGTCGATTGCCGCTTACAACGGTAGCTTTTCTGGTAACTCCGGTCTCTGCAGCACGGACGTAAGCTCATTCCCGCGGTGTTCTTCTAGATCGGGGATGTCCAAGGATGTCCGGACGCTAATTATTTGCTTCTCGGTAGGTTTAGCAATCCTGCTTGCGTCCCTCACATGCTTCTTGTTCTTAAAGAAGGGAGAAAAGGACGAAGACCGTTCATTAAAGGAGGAATCTTGGGATGTGAAGTCTTTCCATGTGATGAGCTTCACTGAGGGTGAGATTCTTGATTCCATTAAGCAAGAGAATCTTATCGGAAAAGGGGGTTCCGGAAATGTTTATAGAGTTTTACTTGCGAATGGCAAAGAACTCGCCGTAAAACATATATGGAATACCAATCCAAGTGGCAGGAAAAAGTTCGAGAGCACGACCCCAATGCTTGCAAAACGAGGTGGGAAGTCCAAGGAATTCGACGCTGAGGTGCAGACGTTGAGCTCAATTAGGCATGTGAATGTGGTGAAGTTGTACTGCAGCATTACCAGCGAGGATTCGAGCTTGTTGGTGTATGAGTACATGCCGAATGGAAGCTTGTGGGATCGGCTTCATACATGCCAGAAGATGAAGCTTGATTGGGAGACAAGGCATGAGATAGCAGTGGGAGCAGCCAAAGGGTTGGAGTATTTACATCATGGCTTGGAGAGGCTGGTGATACACAGAGATGTCAAGTCTAGTAACATTTTATTGGATGAGTTTTTGAAGCCGAGAATTGCGGATTTTGGGCTTGCCAAGATTGTTCAGGCCACTGCAGGCAAGGACTCTACTCACGTTATTGCCGGAACACATGGCTACATTGCTCCTGAATATGGATACACGTACAAAGTGAACGAGAAGAGTGATGTGTACAGCTTTGGAGTGGTACTAATGGAGCTAGTGACGGGGAAAAGGCCGATAGAGGCAGAGTTCGGGGAGAACAAGGACATAGTGAGCTGGGTAAGCAGCATGCTCAAGAGTAGAGAGAGCATATTAAGTATGGTGGACTCATATATTCCAGAGGTGTACAAGGAAGAGGCTATCAAGGTGTTAAGAATTGCAGTTCTGTGCACGGCTAGGCTGCCGGAGCTAAGACCCTCCATGAGAAGTGTGGTTCAAATGCTAGAAGAGGCTCACGAGACGTTTAAATTGCTCAAAATTGTTATCGGCAAAGATGATGCCTCTGGTAATAAGAAAATGGAACTAGTGAAGGGTATAGAGAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001653 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009791 GO:0009845 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045087 GO:0046777 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0090351 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

987

Amino Acids

108.52

Weight (kDa)

6.25

Isoelectric Point (pI)

31.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	29 - 69	5.5e-08	Leucine rich repeat N-terminal domain
LRR_14	PF23598	165 - 323	3.4e-12	Leucine-rich repeat region
Pkinase	PF00069	669 - 951	1.2e-43	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	669 - 953	5e-38	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004091)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09970 AT1G09970
fragaria_vesca	FvH4_5g33590
malus_domestica	MD08G1210000.v1.1 MD15G1394100.v1.1
prunus_persica	Prupe.1G542300_v2.0.a1
pyrus_communis	pycom08g18120 pycom15g35400 pycom15g35410
rosa_chinensis	RchiOBHm_Chr7g0217331 RchiOBHm_Chr7g0232471
rosa_laevigata	RLG00000001335 RLG00000002489 RLG00000002493
rosa_multiflora	Rmu_sc0001898.1_g000015 Rmu_sc0004663.1_g000011 Rmu_sc0011824.1_g000016
rosa_roxburghii	Rroxscaffold_3G00228280 Rroxscaffold_3G00242430
rosa_rugosa	Rorug07G0168100 Rorug07G0274700
rosa_samantha	Rh7AG307400 Rh7AG307800 Rh7AG428100 Rh7BG298200 Rh7BG402600 Rh7CG326500 Rh7CG326900 Rh7CG447100 Rh7DG306900 Rh7DG418900
rosa_wichuraiana	Rw7G026090 Rw7G035470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1308
Acc36I	ACCTGC	1 cut(s)	925
AccB1I	GGYRCC	1 cut(s)	1697
AccI	GTMKAC	1 cut(s)	11
AccII	CGCG	7 cut(s)	80, 122, 177, 824, 1295, 1612, 1789
AccIII	TCCGGA	2 cut(s)	1824, 2033
AclWI	GGATC	4 cut(s)	412, 622, 1657, 2307
AcoI	YGGCCR	1 cut(s)	84
AcsI	RAATTY	7 cut(s)	26, 364, 932, 1128, 1212, 1603, 2171
AcuI	CTGAAG	2 cut(s)	80, 599
AcyI	GRCGYC	2 cut(s)	816, 1698
AfaI	GTAC	9 cut(s)	374, 475, 962, 2231, 2276, 2579, 2606, 2622, 2762
AfiI	CCNNNNNNNGG	4 cut(s)	432, 927, 1048, 2567
AflIII	ACRYGT	2 cut(s)	1473, 2574
AgeI	ACCGGT	1 cut(s)	977
AhlI	ACTAGT	1 cut(s)	2938
AjnI	CCWGG	1 cut(s)	1207
AjuI	GAANNNNNNNTTGG	4 cut(s)	134, 166, 1004, 1036
Alw21I	GWGCWC	4 cut(s)	747, 2132, 2201, 2807
Alw26I	GTCTC	5 cut(s)	525, 1036, 1763, 2333, 2867
Alw44I	GTGCAC	1 cut(s)	2803
AlwI	GGATC	4 cut(s)	412, 622, 1657, 2307
Ama87I	CYCGRG	2 cut(s)	740, 1420
Aor13HI	TCCGGA	2 cut(s)	1824, 2033
AoxI	GGCC	7 cut(s)	16, 84, 385, 781, 1267, 2502, 2648
ApaLI	GTGCAC	1 cut(s)	2803
ApeKI	GCWGC	9 cut(s)	183, 612, 736, 1345, 1764, 2235, 2366, 2700, 2815
ApoI	RAATTY	7 cut(s)	26, 364, 932, 1128, 1212, 1603, 2171
ArsI	GACNNNNNNTTYG	6 cut(s)	524, 556, 1444, 1476, 1700, 1732
AsiGI	ACCGGT	1 cut(s)	977
Asp700I	GAANNNNTTC	5 cut(s)	444, 899, 1166, 1955, 2120
AspA2I	CCTAGG	1 cut(s)	784
AspLEI	GCGC	2 cut(s)	1309, 1700
AspS9I	GGNCC	6 cut(s)	346, 385, 623, 980, 1008, 1268
AsuC2I	CCSGG	5 cut(s)	256, 433, 766, 1421, 1422
AsuII	TTCGAA	1 cut(s)	239
AvaI	CYCGRG	2 cut(s)	740, 1420
AvaII	GGWCC	4 cut(s)	346, 623, 980, 1008
AvrII	CCTAGG	1 cut(s)	784
BaeGI	GKGCMC	1 cut(s)	2807
BaeI	ACNNNNGTAYC	2 cut(s)	1481, 1514
BanI	GGYRCC	1 cut(s)	1697
BanII	GRGCYC	2 cut(s)	747, 2201
BauI	CACGAG	1 cut(s)	2870
BbsI	GAAGAC	2 cut(s)	1304, 1923
Bbv12I	GWGCWC	4 cut(s)	747, 2132, 2201, 2807
BbvCI	CCTCAGC	1 cut(s)	2181
BbvI	GCAGC	9 cut(s)	195, 624, 748, 1332, 1776, 2247, 2378, 2712, 2802
BccI	CCATC	4 cut(s)	39, 712, 1072, 1573
BceAI	ACGGC	2 cut(s)	2063, 2823
BcgI	CGANNNNNNTGC	4 cut(s)	2056, 2090, 2122, 2156
BciT130I	CCWGG	1 cut(s)	1209
BciVI	GTATCC	1 cut(s)	2564
BclI	TGATCA	1 cut(s)	544
BcnI	CCSGG	5 cut(s)	256, 433, 766, 1421, 1422
BcoDI	GTCTC	5 cut(s)	525, 1036, 1763, 2333, 2867
BcuI	ACTAGT	1 cut(s)	2938
BfaI	CTAG	7 cut(s)	785, 1800, 2433, 2633, 2811, 2858, 2939
BfmI	CTRYAG	3 cut(s)	1762, 2233, 2508
BfoI	RGCGCY	1 cut(s)	1701
BfuAI	ACCTGC	1 cut(s)	925
BfuI	GTATCC	1 cut(s)	2564
BglI	GCCNNNNNGGC	1 cut(s)	83
BlnI	CCTAGG	1 cut(s)	784
BlpI	GCTNAGC	1 cut(s)	1661
BmcAI	AGTACT	1 cut(s)	374
Bme1390I	CCNGG	6 cut(s)	256, 433, 766, 1209, 1421, 1422
Bme18I	GGWCC	4 cut(s)	346, 623, 980, 1008
BmeT110I	CYCGRG	2 cut(s)	740, 1420
BmgT120I	GGNCC	6 cut(s)	346, 385, 623, 980, 1008, 1268
BmiI	GGNNCC	2 cut(s)	1699, 2032
BmrFI	CCNGG	6 cut(s)	256, 433, 766, 1209, 1421, 1422
BmsI	GCATC	2 cut(s)	1453, 2902
BpiI	GAAGAC	2 cut(s)	1304, 1923
BpmI	CTGGAG	1 cut(s)	1091
Bpu10I	CCTNAGC	2 cut(s)	1645, 2181
Bpu1102I	GCTNAGC	1 cut(s)	1661
Bpu14I	TTCGAA	1 cut(s)	239
BpuEI	CTTGAG	1 cut(s)	2693
BpuMI	CCSGG	5 cut(s)	256, 433, 766, 1421, 1422
Bsa29I	ATCGAT	1 cut(s)	231
BsaAI	YACGTR	1 cut(s)	2577
BsaBI	GATNNNNATC	1 cut(s)	231
BsaHI	GRCGYC	2 cut(s)	816, 1698
BsaI	GGTCTC	1 cut(s)	1763
BsaWI	WCCGGW	8 cut(s)	191, 905, 977, 1193, 1553, 1754, 1824, 2033
Bsc4I	CCNNNNNNNGG	4 cut(s)	432, 927, 1048, 2567
Bse118I	RCCGGY	3 cut(s)	977, 1121, 1694
Bse1I	ACTGG	2 cut(s)	171, 596
Bse3DI	GCAATG	2 cut(s)	1533, 2553
Bse8I	GATNNNNATC	1 cut(s)	231
BseAI	TCCGGA	2 cut(s)	1824, 2033
BseBI	CCWGG	1 cut(s)	1209
BseCI	ATCGAT	1 cut(s)	231
BseGI	GGATG	8 cut(s)	45, 422, 704, 1357, 1815, 1825, 1951, 2455
BseJI	GATNNNNATC	1 cut(s)	231
BseLI	CCNNNNNNNGG	4 cut(s)	432, 927, 1048, 2567
BseMI	GCAATG	2 cut(s)	1533, 2553
BseMII	CTCAG	9 cut(s)	414, 512, 845, 885, 1004, 1026, 1511, 1968, 2172
BseNI	ACTGG	2 cut(s)	171, 596
BseRI	GAGGAG	2 cut(s)	47, 59
BseSI	GKGCMC	1 cut(s)	2807
BseX3I	CGGCCG	1 cut(s)	84
BseXI	GCAGC	9 cut(s)	195, 624, 748, 1332, 1776, 2247, 2378, 2712, 2802
BseYI	CCCAGC	1 cut(s)	2691
BsgI	GTGCAG	1 cut(s)	2207
Bsh1236I	CGCG	7 cut(s)	80, 122, 177, 824, 1295, 1612, 1789
Bsh1285I	CGRYCG	1 cut(s)	87
BshFI	GGCC	7 cut(s)	18, 86, 387, 783, 1269, 2504, 2650
BshNI	GGYRCC	1 cut(s)	1697
BshTI	ACCGGT	1 cut(s)	977
BshVI	ATCGAT	1 cut(s)	231
BsiEI	CGRYCG	1 cut(s)	87
BsiHKAI	GWGCWC	4 cut(s)	747, 2132, 2201, 2807
BsiHKCI	CYCGRG	2 cut(s)	740, 1420
BslFI	GGGAC	5 cut(s)	346, 415, 1096, 1465, 1858
BslI	CCNNNNNNNGG	4 cut(s)	432, 927, 1048, 2567
BsmAI	GTCTC	5 cut(s)	525, 1036, 1763, 2333, 2867
BsmBI	CGTCTC	1 cut(s)	2867
BsmFI	GGGAC	5 cut(s)	346, 415, 1096, 1465, 1858
BsmI	GAATGC	1 cut(s)	1234
BsnI	GGCC	7 cut(s)	18, 86, 387, 783, 1269, 2504, 2650
Bso31I	GGTCTC	1 cut(s)	1763
BsoBI	CYCGRG	2 cut(s)	740, 1420
Bsp119I	TTCGAA	1 cut(s)	239
Bsp1286I	GDGCHC	4 cut(s)	747, 2132, 2201, 2807
Bsp13I	TCCGGA	2 cut(s)	1824, 2033
Bsp1407I	TGTACA	3 cut(s)	960, 2604, 2760
Bsp143I	GATC	7 cut(s)	232, 404, 544, 627, 1649, 1802, 2299
Bsp1720I	GCTNAGC	1 cut(s)	1661
Bsp19I	CCATGG	1 cut(s)	482
Bsp68I	TCGCGA	1 cut(s)	1612
BspANI	GGCC	7 cut(s)	18, 86, 387, 783, 1269, 2504, 2650
BspCNI	CTCAG	9 cut(s)	413, 513, 846, 884, 1003, 1027, 1512, 1969, 2173
BspDI	ATCGAT	1 cut(s)	231
BspEI	TCCGGA	2 cut(s)	1824, 2033
BspFNI	CGCG	7 cut(s)	80, 122, 177, 824, 1295, 1612, 1789
BspLI	GGNNCC	2 cut(s)	1699, 2032
BspMAI	CTGCAG	3 cut(s)	1766, 2237, 2512
BspMI	ACCTGC	1 cut(s)	925
BspPI	GGATC	4 cut(s)	412, 622, 1657, 2307
BspQI	GCTCTTC	1 cut(s)	1634
BspT104I	TTCGAA	1 cut(s)	239
BspT107I	GGYRCC	1 cut(s)	1697
BspTNI	GGTCTC	1 cut(s)	1763
BsrDI	GCAATG	2 cut(s)	1533, 2553
BsrFI	RCCGGY	3 cut(s)	977, 1121, 1694
BsrGI	TGTACA	3 cut(s)	960, 2604, 2760
BsrI	ACTGG	2 cut(s)	171, 596
BssAI	RCCGGY	3 cut(s)	977, 1121, 1694
BssMI	GATC	7 cut(s)	232, 404, 544, 627, 1649, 1802, 2299
BssNI	GRCGYC	2 cut(s)	816, 1698
BssSI	CACGAG	1 cut(s)	2870
BssT1I	CCWWGG	7 cut(s)	127, 309, 482, 784, 1264, 1815, 2166
Bst2BI	CACGAG	1 cut(s)	2870
Bst2UI	CCWGG	1 cut(s)	1209
Bst4CI	ACNGT	2 cut(s)	1736, 1922
Bst6I	CTCTTC	6 cut(s)	56, 1587, 1634, 2588, 2763, 2856
BstACI	GRCGYC	2 cut(s)	816, 1698
BstAUI	TGTACA	3 cut(s)	960, 2604, 2760
BstBAI	YACGTR	1 cut(s)	2577
BstBI	TTCGAA	1 cut(s)	239
BstC8I	GCNNGC	9 cut(s)	202, 284, 1305, 1343, 1869, 2145, 2487, 2512, 2705
BstDSI	CCRYGG	3 cut(s)	78, 482, 1787
BstF5I	GGATG	8 cut(s)	45, 422, 704, 1357, 1815, 1825, 1951, 2455
BstFNI	CGCG	7 cut(s)	80, 122, 177, 824, 1295, 1612, 1789
BstH2I	RGCGCY	1 cut(s)	1701
BstHHI	GCGC	2 cut(s)	1309, 1700
BstKTI	GATC	7 cut(s)	235, 407, 547, 630, 1652, 1805, 2302
BstMAI	GTCTC	5 cut(s)	525, 1036, 1763, 2333, 2867
BstMBI	GATC	7 cut(s)	232, 404, 544, 627, 1649, 1802, 2299
BstMCI	CGRYCG	1 cut(s)	87
BstNI	CCWGG	1 cut(s)	1209
BstNSI	RCATGY	6 cut(s)	1477, 1884, 2213, 2281, 2316, 2707
BstSCI	CCNGG	6 cut(s)	254, 431, 764, 1207, 1419, 1420
BstSFI	CTRYAG	3 cut(s)	1762, 2233, 2508
BstSLI	GKGCMC	1 cut(s)	2807
BstUI	CGCG	7 cut(s)	80, 122, 177, 824, 1295, 1612, 1789
BstV1I	GCAGC	9 cut(s)	195, 624, 748, 1332, 1776, 2247, 2378, 2712, 2802
BstV2I	GAAGAC	2 cut(s)	1304, 1923
BstX2I	RGATCY	1 cut(s)	1649
BstXI	CCANNNNNNTGG	1 cut(s)	2377
BstYI	RGATCY	1 cut(s)	1649
BstZI	CGGCCG	1 cut(s)	84
Bsu15I	ATCGAT	1 cut(s)	231
BsuI	GTATCC	1 cut(s)	2564
BsuRI	GGCC	7 cut(s)	18, 86, 387, 783, 1269, 2504, 2650
BsuTUI	ATCGAT	1 cut(s)	231
BtgI	CCRYGG	3 cut(s)	78, 482, 1787
BtsCI	GGATG	8 cut(s)	45, 422, 704, 1357, 1815, 1825, 1951, 2455
BtsI	GCAGTG	2 cut(s)	2364, 2505
BtsIMutI	CAGTG	3 cut(s)	1974, 2364, 2505
BtuMI	TCGCGA	1 cut(s)	1612
BveI	ACCTGC	1 cut(s)	925
Cac8I	GCNNGC	9 cut(s)	202, 284, 1305, 1343, 1869, 2145, 2487, 2512, 2705
CfoI	GCGC	2 cut(s)	1309, 1700
Cfr10I	RCCGGY	3 cut(s)	977, 1121, 1694
Cfr13I	GGNCC	6 cut(s)	346, 385, 623, 980, 1008, 1268
Cfr42I	CCGCGG	2 cut(s)	81, 1790
Cfr9I	CCCGGG	1 cut(s)	1420
ClaI	ATCGAT	1 cut(s)	231
CseI	GACGC	6 cut(s)	281, 824, 1309, 1837, 1860, 2186
Csp6I	GTAC	9 cut(s)	373, 474, 961, 2230, 2275, 2578, 2605, 2621, 2761
CspAI	ACCGGT	1 cut(s)	977
CspCI	CAANNNNNGTGG	2 cut(s)	2089, 2124
CviQI	GTAC	9 cut(s)	373, 474, 961, 2230, 2275, 2578, 2605, 2621, 2761
DinI	GGCGCC	1 cut(s)	1699
DpnI	GATC	7 cut(s)	234, 406, 546, 629, 1651, 1804, 2301
DpnII	GATC	7 cut(s)	232, 404, 544, 627, 1649, 1802, 2299
DraI	TTTAAA	1 cut(s)	2881
EaeI	YGGCCR	1 cut(s)	84
EagI	CGGCCG	1 cut(s)	84
Eam1104I	CTCTTC	6 cut(s)	56, 1587, 1634, 2588, 2763, 2856
EarI	CTCTTC	6 cut(s)	56, 1587, 1634, 2588, 2763, 2856
EciI	GGCGGA	3 cut(s)	64, 284, 1050
Ecl136II	GAGCTC	2 cut(s)	745, 2199
EclXI	CGGCCG	1 cut(s)	84
Eco130I	CCWWGG	7 cut(s)	127, 309, 482, 784, 1264, 1815, 2166
Eco24I	GRGCYC	2 cut(s)	747, 2201
Eco31I	GGTCTC	1 cut(s)	1763
Eco32I	GATATC	2 cut(s)	229, 1561
Eco47I	GGWCC	4 cut(s)	346, 623, 980, 1008
Eco52I	CGGCCG	1 cut(s)	84
Eco53kI	GAGCTC	2 cut(s)	745, 2199
Eco57I	CTGAAG	2 cut(s)	80, 599
Eco88I	CYCGRG	2 cut(s)	740, 1420
EcoICRI	GAGCTC	2 cut(s)	745, 2199
EcoO109I	RGGNCCY	1 cut(s)	346
EcoRI	GAATTC	2 cut(s)	932, 2171
EcoRII	CCWGG	1 cut(s)	1207
EcoRV	GATATC	2 cut(s)	229, 1561
EcoT14I	CCWWGG	7 cut(s)	127, 309, 482, 784, 1264, 1815, 2166
EcoT38I	GRGCYC	2 cut(s)	747, 2201
EgeI	GGCGCC	1 cut(s)	1699
EheI	GGCGCC	1 cut(s)	1699
ErhI	CCWWGG	7 cut(s)	127, 309, 482, 784, 1264, 1815, 2166
Esp3I	CGTCTC	1 cut(s)	2867
FalI	AAGNNNNNCTT	2 cut(s)	1998, 2030
FaqI	GGGAC	5 cut(s)	346, 415, 1096, 1465, 1858
FauI	CCCGC	1 cut(s)	1794
FbaI	TGATCA	1 cut(s)	544
FblI	GTMKAC	1 cut(s)	11
FokI	GGATG	8 cut(s)	32, 409, 691, 1364, 1822, 1832, 1958, 2462
FriOI	GRGCYC	2 cut(s)	747, 2201
FspBI	CTAG	7 cut(s)	785, 1800, 2433, 2633, 2811, 2858, 2939
FspI	TGCGCA	1 cut(s)	1308
GlaI	GCGC	2 cut(s)	1308, 1699
GsaI	CCCAGC	1 cut(s)	2695
GsuI	CTGGAG	1 cut(s)	1091
HaeII	RGCGCY	1 cut(s)	1701
HaeIII	GGCC	7 cut(s)	18, 86, 387, 783, 1269, 2504, 2650
HgaI	GACGC	6 cut(s)	281, 824, 1309, 1837, 1860, 2186
HhaI	GCGC	2 cut(s)	1309, 1700
Hin1I	GRCGYC	2 cut(s)	816, 1698
Hin6I	GCGC	2 cut(s)	1307, 1698
HinP1I	GCGC	2 cut(s)	1307, 1698
HincII	GTYRAC	2 cut(s)	12, 1177
HindII	GTYRAC	2 cut(s)	12, 1177
HindIII	AAGCTT	3 cut(s)	941, 2290, 2327
HpaI	GTTAAC	1 cut(s)	1177
Hpy166II	GTNNAC	8 cut(s)	12, 963, 1177, 2587, 2605, 2740, 2761, 2805
Hpy8I	GTNNAC	8 cut(s)	12, 963, 1177, 2587, 2605, 2740, 2761, 2805
Hpy99I	CGWCG	6 cut(s)	10, 13, 92, 818, 1159, 2180
HpyAV	CCTTC	6 cut(s)	628, 786, 830, 1676, 1894, 2938
HpyCH4III	ACNGT	2 cut(s)	1736, 1922
HpyCH4IV	ACGT	6 cut(s)	1157, 1773, 2192, 2529, 2576, 2876
HpySE526I	ACGT	6 cut(s)	1157, 1773, 2192, 2529, 2576, 2876
Hsp92I	GRCGYC	2 cut(s)	816, 1698
HspAI	GCGC	2 cut(s)	1307, 1698
KasI	GGCGCC	1 cut(s)	1697
Kpn2I	TCCGGA	2 cut(s)	1824, 2033
Ksp22I	TGATCA	1 cut(s)	544
KspAI	GTTAAC	1 cut(s)	1177
KspI	CCGCGG	2 cut(s)	81, 1790
Kzo9I	GATC	7 cut(s)	232, 404, 544, 627, 1649, 1802, 2299
LguI	GCTCTTC	1 cut(s)	1634
LmnI	GCTCC	7 cut(s)	655, 850, 1110, 2363, 2563, 2629, 2821
Lsp1109I	GCAGC	9 cut(s)	195, 624, 748, 1332, 1776, 2247, 2378, 2712, 2802
LweI	GCATC	2 cut(s)	1453, 2902
MaeI	CTAG	7 cut(s)	785, 1800, 2433, 2633, 2811, 2858, 2939
MaeII	ACGT	6 cut(s)	1157, 1773, 2192, 2529, 2576, 2876
MaeIII	GTNAC	7 cut(s)	874, 1322, 1433, 1523, 1748, 2435, 2635
MalI	GATC	7 cut(s)	234, 406, 546, 629, 1651, 1804, 2301
MboI	GATC	7 cut(s)	232, 404, 544, 627, 1649, 1802, 2299
MflI	RGATCY	1 cut(s)	1649
MhlI	GDGCHC	4 cut(s)	747, 2132, 2201, 2807
Mly113I	GGCGCC	1 cut(s)	1698
MlyI	GAGTC	4 cut(s)	825, 1447, 2513, 2735
MmeI	TCCRAC	5 cut(s)	673, 828, 1178, 1610, 2358
MroI	TCCGGA	2 cut(s)	1824, 2033
MroXI	GAANNNNTTC	5 cut(s)	444, 899, 1166, 1955, 2120
MseI	TTAA	8 cut(s)	1176, 1529, 1895, 1928, 2001, 2729, 2786, 2880
MslI	CAYNNNNRTG	2 cut(s)	2214, 2421
MspA1I	CMGCKG	3 cut(s)	80, 462, 1789
MspR9I	CCNGG	6 cut(s)	256, 433, 766, 1209, 1421, 1422
Mva1269I	GAATGC	1 cut(s)	1234
MvaI	CCWGG	1 cut(s)	1209
MvnI	CGCG	7 cut(s)	80, 122, 177, 824, 1295, 1612, 1789
NarI	GGCGCC	1 cut(s)	1698
NciI	CCSGG	5 cut(s)	256, 433, 766, 1421, 1422
NcoI	CCATGG	1 cut(s)	482
NdeII	GATC	7 cut(s)	232, 404, 544, 627, 1649, 1802, 2299
NlaIV	GGNNCC	2 cut(s)	1699, 2032
NmeAIII	GCCGAG	2 cut(s)	946, 2490
NmuCI	GTSAC	3 cut(s)	1433, 1523, 2635
NruI	TCGCGA	1 cut(s)	1612
NsbI	TGCGCA	1 cut(s)	1308
NspI	RCATGY	6 cut(s)	1477, 1884, 2213, 2281, 2316, 2707
NspV	TTCGAA	1 cut(s)	239
PaeI	GCATGC	1 cut(s)	2707
PaeR7I	CTCGAG	1 cut(s)	740
PciI	ACATGT	1 cut(s)	1473
PciSI	GCTCTTC	1 cut(s)	1634
PcsI	WCGNNNNNNNCGW	2 cut(s)	747, 810
PctI	GAATGC	1 cut(s)	1234
PdmI	GAANNNNTTC	5 cut(s)	444, 899, 1166, 1955, 2120
PfoI	TCCNGGA	1 cut(s)	1207
PinAI	ACCGGT	1 cut(s)	977
PleI	GAGTC	4 cut(s)	825, 1447, 2513, 2735
PluTI	GGCGCC	1 cut(s)	1701
PpsI	GAGTC	4 cut(s)	825, 1447, 2513, 2735
Ppu21I	YACGTR	1 cut(s)	2577
PpuMI	RGGWCCY	1 cut(s)	346
PscI	ACATGT	1 cut(s)	1473
Psp124BI	GAGCTC	2 cut(s)	747, 2201
Psp5II	RGGWCCY	1 cut(s)	346
Psp6I	CCWGG	1 cut(s)	1207
PspFI	CCCAGC	1 cut(s)	2691
PspGI	CCWGG	1 cut(s)	1207
PspN4I	GGNNCC	2 cut(s)	1699, 2032
PspPI	GGNCC	6 cut(s)	346, 385, 623, 980, 1008, 1268
PspPPI	RGGWCCY	1 cut(s)	346
PspXI	VCTCGAGB	1 cut(s)	740
PstI	CTGCAG	3 cut(s)	1766, 2237, 2512
PsuI	RGATCY	1 cut(s)	1649
RruI	TCGCGA	1 cut(s)	1612
RsaI	GTAC	9 cut(s)	374, 475, 962, 2231, 2276, 2579, 2606, 2622, 2762
RsaNI	GTAC	9 cut(s)	373, 474, 961, 2230, 2275, 2578, 2605, 2621, 2761
RseI	CAYNNNNRTG	2 cut(s)	2214, 2421
SacI	GAGCTC	2 cut(s)	747, 2201
SacII	CCGCGG	2 cut(s)	81, 1790
SalI	GTCGAC	1 cut(s)	10
SapI	GCTCTTC	1 cut(s)	1634
SaqAI	TTAA	8 cut(s)	1176, 1529, 1895, 1928, 2001, 2729, 2786, 2880
Sau3AI	GATC	7 cut(s)	232, 404, 544, 627, 1649, 1802, 2299
Sau96I	GGNCC	6 cut(s)	346, 385, 623, 980, 1008, 1268
ScaI	AGTACT	1 cut(s)	374
SchI	GAGTC	4 cut(s)	825, 1447, 2513, 2735
ScrFI	CCNGG	6 cut(s)	256, 433, 766, 1209, 1421, 1422
SduI	GDGCHC	4 cut(s)	747, 2132, 2201, 2807
SfaNI	GCATC	2 cut(s)	1453, 2902
SfcI	CTRYAG	3 cut(s)	1762, 2233, 2508
SfoI	GGCGCC	1 cut(s)	1699
Sfr274I	CTCGAG	1 cut(s)	740
Sfr303I	CCGCGG	2 cut(s)	81, 1790
SfuI	TTCGAA	1 cut(s)	239
SgrAI	CRCCGGYG	1 cut(s)	1694
SgrBI	CCGCGG	2 cut(s)	81, 1790
SinI	GGWCC	4 cut(s)	346, 623, 980, 1008
SlaI	CTCGAG	1 cut(s)	740
SmaI	CCCGGG	1 cut(s)	1422
SmiMI	CAYNNNNRTG	2 cut(s)	2214, 2421
SmlI	CTYRAG	2 cut(s)	740, 2708
SmoI	CTYRAG	2 cut(s)	740, 2708
SpeI	ACTAGT	1 cut(s)	2938
SphI	GCATGC	1 cut(s)	2707
SspDI	GGCGCC	1 cut(s)	1697
SspI	AATATT	1 cut(s)	1429
SspMI	CTAG	7 cut(s)	785, 1800, 2433, 2633, 2811, 2858, 2939
SstI	GAGCTC	2 cut(s)	747, 2201
StyD4I	CCNGG	6 cut(s)	254, 431, 764, 1207, 1419, 1420
StyI	CCWWGG	7 cut(s)	127, 309, 482, 784, 1264, 1815, 2166
TaaI	ACNGT	2 cut(s)	1736, 1922
TaiI	ACGT	6 cut(s)	1160, 1776, 2195, 2532, 2579, 2879
TatI	WGTACW	6 cut(s)	372, 960, 2229, 2274, 2604, 2760
TauI	GCSGC	5 cut(s)	80, 83, 86, 269, 1727
Tru1I	TTAA	8 cut(s)	1176, 1529, 1895, 1928, 2001, 2729, 2786, 2880
Tru9I	TTAA	8 cut(s)	1176, 1529, 1895, 1928, 2001, 2729, 2786, 2880
TscAI	CASTG	3 cut(s)	1981, 2364, 2512
TseFI	GTSAC	3 cut(s)	1433, 1523, 2635
TseI	GCWGC	9 cut(s)	183, 612, 736, 1345, 1764, 2235, 2366, 2700, 2815
Tsp45I	GTSAC	3 cut(s)	1433, 1523, 2635
TspDTI	ATGAA	5 cut(s)	437, 1012, 1914, 2297, 2339
TspGWI	ACGGA	3 cut(s)	228, 668, 1784
TspMI	CCCGGG	1 cut(s)	1420
TspRI	CASTG	3 cut(s)	1981, 2364, 2512
VneI	GTGCAC	1 cut(s)	2803
VpaK11BI	GGWCC	4 cut(s)	346, 623, 980, 1008
XapI	RAATTY	7 cut(s)	26, 364, 932, 1128, 1212, 1603, 2171
XbaI	TCTAGA	1 cut(s)	1799
XceI	RCATGY	6 cut(s)	1477, 1884, 2213, 2281, 2316, 2707
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2842
XhoI	CTCGAG	1 cut(s)	740
XmaI	CCCGGG	1 cut(s)	1420
XmaJI	CCTAGG	1 cut(s)	784
XmiI	GTMKAC	1 cut(s)	11
XmnI	GAANNNNTTC	5 cut(s)	444, 899, 1166, 1955, 2120
XspI	CTAG	7 cut(s)	785, 1800, 2433, 2633, 2811, 2858, 2939
ZrmI	AGTACT	1 cut(s)	374

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.