Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0004091

Physical Location & Seq

Genomic Coordinates Reverse (-)

53023959 .. 53027906

Gene Structure 3948 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G035470.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3042 bp

ATGGTTAAAATGGCATACTCATGGAGTCTCACTAACGCATTCCCATCTTCAAAGTCCATTCACCCACTCTTCGTCTGTCAACTCCATCATGCCATTACCCAAAAACCACCACCTCCATCTCTACCTCCTCTTCTTCCTCTTCCTCCTCTCCAACGCCGCCGCAGCCGACGAGCTTCAAATCCTACTAAAACTCAAATCCTCCCTCCAGGACTCAAACTCCCAACCTTCGCCACATGGGACTCCACCGCCAATTCGTTTTGCAAATTCACCGGAATCACCTGCAACGTCGTCGGTTCGGTTCGAGAAATCGAACTTTCGAACCAGAAGCTGTCGGGGTCTCTTCCGTTGGACTCCATATGCCAACTTCCCTCATTAGAAAAGCTCGCTTTCGGGTCCAATTTCCTGCACGGTACAATCACGGAGGACTTGAGAAACTGTACGAAGCTGAAATACCTGGATTTGGGGAAAAACTTGTTCGCGGGTTCATTCCCTGACATATCTTCCTTGTCCCAACTGGAGCATCTTCATCTGAACGGCAGCTGGTTTTCCGGGATTTTCCCGTGGACTTCCCTCACCAACATGACTGGTCTGATTCGTTTGAGCTTGGGTGACAACCCGTTTGATCCCAGCCCGTTTCCAAAGGAAGTTGTAAATCTTAACAAGCTCGAGTGGCTCTACTTGGCAAACTGCAGCATCCAAGGAACAATACCAAGTGAAATCGGGAACTTGGTCGAGCTTATCAACCTGGAGTTGTCCGATAACAACATGACCGGAGAAATTCCGGCCGAGATTGGACAGCTCACCAAGCTCTGGCAGCTGGAGCTCTACACCAACGGGTTCACCGGAAAGCTTCCTTTCGGGCTAAGAAACCTCACCATTCTCGAGAACTTCGACGCCTCTGAGAATTTTCTGGAAGGCGATTTGAACGAGTTGAGGTTTCTGACCAACTTGGTTTCTCTGCAGCTGTATGACAACAATTTTTCCGGCGAAGTACCGGAAGAGTTCGGCGAATTAAAGAAGCTTGTGAATCTGTCTTTGTACGGTAACAAGCTGACCGGTCCTCTGCCTCAGAAATTGGGTTCTTGGTCGGAGATTGACTTCATCGACGTGTCGGAGAACTTCTTGACCGGGACTATTCCGCCGGACATGTGCAACAAAGGGACGATGAAGCAGCTGCTCATGCTTCAGAACCAATTAACTGGCGAAATTCCGGCAAACTACGCTAAATGCACGACGTTGACCCGGTTCAGGGTCAACAACAACTCCCTCTCCGGTGTGGTTCCGGCAGGACTTTGGGGATTGCCGAATGTGGAAATCATTGACATCACTTCGAATCAAATTGAAGGGCCGATTACTTCCGATATCGGAAACGCCAAGAAGCTTGCGCAGTTGTTTGTGAGGTACAATCGGTTATCTGGTGAATTACCGGACGAGCTTTCGAAGTCAACTTCGTTGGTTTCGGTTATGTTGAATAACAATCAGTTTTCCGGTAAGATTCCGGCGAAGATAGGGGACTTGAAGCAATTGGGTACTCTGCATTTAGAGAACAACTTGTTGTCTTCTTCGATTCCCAAGTCATTGGGAAGCTGTAGTTTCCTCAGTGACTTGAACATGGCGAACAACTCGCTTTCCGGTGAAATCCCATCATCTTTGGGCTCTTTGCCGACCTTGAATTCTCTGGATTTGTCTCACAACCAACTCTCCGGAAAAATCCCGGAAAGTCTAGCATCTCTGAGACTAAGCATGCTTGATCTTTCGCACAACAGACTGACCGGTGCCGTACCGGAATCTCTCTCGATTGCAGCCTACAACGGCAGCCTTTCCGGTAACCCGGGTCTCTGCAGCATGGACATCACCTACTTCCCACGGTGCTCGCCGAAAAAGGAAATGTCCGACGATGTCAGGACACTCATTATTTGCTTCTCAGTGGGTACAGCAATATTGTTTGTTTCACTCATTTGCTTCTTGTTCTTAAAGGGGAAGGAGAAAGATCAAGACCGTTCATTGAAGGAAGAATCTTGGGATGTAAAGTCTTTCCATGTAATAACCTTCAGTGAGGATGAGATTCTTGATTCCATTACTCAAGAGAATCTAATTGGAAAAGGAGGTTCTGGAAATGTCTACAAAGTGTCACTAGACAATGGCAAAGAACTCGCCGTGAAGCACATATGGAACACTGATCCAAGCGGCCGGAAAATGTTCAAGAGCACCAACCCGATGCTCGGGAGACGGGGCAGTGGTAGTTCGAAATCGAAGGAATTTGATGCTGAGGTGCAGACGTTGAGCTCAATAAGGCATGTGAATGTGGTGAAGTTGTTCTGCAGCATTACTAGTGAGGACTCGAGCCTTTTGGTCTACGAGTACTTACCGAACGGAAGCTTGTGGGATCGGCTGCACACGTGTGAGAAGATGAAGCTTGATTGGGACGCAAGGTATGAGATTGCAGTCGGAGCAGCCAAAGGGTTGGAGTATCTGCACCATGGCTGTGAGAGGCTAGTGATTCATAGAGATGTCAAGTCGAGTAACATTTTGTTAGATGAGTTTTTGAAGCCCCGGATCGCGGATTTTGGGCTCGCCAAGATGGTTCAGACTAATGCAATTAAGGACTCGACTCATGTTGTTGCTGGAACACACGGTTACATAGCTCCTGAATATGGTTACACCTACAAGGTGAATGAGAAGAGCGATGTGTATAGCTTTGGTGTAGTACTAATGGAGCTAGTGACCGGGAAAAAGCCGATAGATCCATCCTTTGGGGACAACAAGGACATAGTGAACTGGATATGTGGCAACCTGAAGAGTAGAGAGAGCGTGTTGGGTGTGGTGGACTCGTACATTCCTGAGGCCTATAGGGAAGAGGCTATCAAGGTATTGAGAATTGCAATTCTATGCACAGCTAGGCTTCCAGAGCTAAGACCTTCCATGAGAAGTGTCGTTCAGATGCTTGAAGAAGCTCACGAGCCAATGAAATTGCTGGACATTGTTATCAGCAAAGATGGTTCTAGTAAGAAAATGGAAGTACTTAAAGGAACAGAGAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001653 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009791 GO:0009845 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045087 GO:0046777 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0090351 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

1013

Amino Acids

111.96

Weight (kDa)

6.16

Isoelectric Point (pI)

33.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	214 - 349	4.6e-13	Leucine-rich repeat region
LRR_14	PF23598	453 - 577	8.5e-06	Leucine-rich repeat region
LRR_8	PF13855	533 - 590	1.3e-06	Leucine rich repeat
Pkinase	PF00069	695 - 980	2.6e-40	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	696 - 982	4.5e-37	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0004091)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09970 AT1G09970
fragaria_vesca	FvH4_5g33590
malus_domestica	MD08G1210000.v1.1 MD15G1394100.v1.1
prunus_persica	Prupe.1G542300_v2.0.a1
pyrus_communis	pycom08g18120 pycom15g35400 pycom15g35410
rosa_chinensis	RchiOBHm_Chr7g0217331 RchiOBHm_Chr7g0232471
rosa_laevigata	RLG00000001335 RLG00000002489 RLG00000002493
rosa_multiflora	Rmu_sc0001898.1_g000015 Rmu_sc0004663.1_g000011 Rmu_sc0011824.1_g000016
rosa_roxburghii	Rroxscaffold_3G00228280 Rroxscaffold_3G00242430
rosa_rugosa	Rorug07G0168100 Rorug07G0274700
rosa_samantha	Rh7AG307400 Rh7AG307800 Rh7AG428100 Rh7BG298200 Rh7BG402600 Rh7CG326500 Rh7CG326900 Rh7CG447100 Rh7DG306900 Rh7DG418900
rosa_wichuraiana	Rw7G026090 Rw7G035470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	287
Acc16I	TGCGCA	1 cut(s)	1386
Acc36I	ACCTGC	1 cut(s)	287
AccB1I	GGYRCC	1 cut(s)	1775
AccB7I	CCANNNNNTGG	2 cut(s)	810, 2753
AccI	GTMKAC	2 cut(s)	2121, 2355
AccII	CGCG	2 cut(s)	479, 2561
AccIII	TCCGGA	1 cut(s)	1703
AciI	CCGC	7 cut(s)	157, 160, 246, 479, 1139, 2187, 2561
AclWI	GGATC	5 cut(s)	617, 2174, 2394, 2564, 2738
AcoI	YGGCCR	2 cut(s)	783, 2188
AcsI	RAATTY	6 cut(s)	263, 777, 904, 1206, 1672, 2258
AcuI	CTGAAG	3 cut(s)	1169, 2035, 2816
AcvI	CACGTG	1 cut(s)	2400
AcyI	GRCGYC	1 cut(s)	894
AfiI	CCNNNNNNNGG	8 cut(s)	460, 810, 1248, 1249, 2222, 2560, 2654, 2753
AflIII	ACRYGT	4 cut(s)	1107, 1146, 2397, 2399
AgeI	ACCGGT	2 cut(s)	1055, 1772
AhlI	ACTAGT	1 cut(s)	2330
AjiI	CACGTC	1 cut(s)	1108
AjnI	CCWGG	3 cut(s)	205, 453, 744
AleI	CACNNNNGTG	1 cut(s)	2400
Alw21I	GWGCWC	4 cut(s)	825, 1874, 2210, 2288
Alw26I	GTCTC	6 cut(s)	32, 342, 1692, 1729, 1841, 2221
AlwI	GGATC	5 cut(s)	617, 2174, 2394, 2564, 2738
AlwNI	CAGNNNCTG	1 cut(s)	328
Ama87I	CYCGRG	5 cut(s)	665, 881, 1831, 2222, 2341
Aor13HI	TCCGGA	1 cut(s)	1703
AoxI	GGCC	4 cut(s)	783, 1346, 2188, 2844
ApoI	RAATTY	6 cut(s)	263, 777, 904, 1206, 1672, 2258
ArsI	GACNNNNNNTTYG	2 cut(s)	602, 634
AsiGI	ACCGGT	2 cut(s)	1055, 1772
Asp700I	GAANNNNTTC	2 cut(s)	2198, 2315
AspLEI	GCGC	1 cut(s)	1387
AspS9I	GGNCC	3 cut(s)	393, 1058, 1346
AsuC2I	CCSGG	8 cut(s)	550, 1129, 1243, 1715, 1832, 1833, 2554, 2728
AsuII	TTCGAA	4 cut(s)	317, 1331, 1439, 2246
AvaI	CYCGRG	5 cut(s)	665, 881, 1831, 2222, 2341
AvaII	GGWCC	2 cut(s)	393, 1058
AxyI	CCTNAGG	1 cut(s)	2841
BanI	GGYRCC	1 cut(s)	1775
BanII	GRGCYC	4 cut(s)	825, 1658, 2288, 2574
BarI	GAAGNNNNNNTAC	2 cut(s)	434, 466
BauI	CACGAG	1 cut(s)	2957
BbrPI	CACGTG	1 cut(s)	2400
BbsI	GAAGAC	1 cut(s)	1551
Bbv12I	GWGCWC	4 cut(s)	825, 1874, 2210, 2288
BbvCI	CCTCAGC	1 cut(s)	2268
BccI	CCATC	7 cut(s)	52, 93, 124, 1651, 2575, 2755, 2990
BceAI	ACGGC	4 cut(s)	550, 1763, 1828, 2141
BcgI	CGANNNNNNTGC	4 cut(s)	271, 305, 2134, 2168
BciT130I	CCWGG	3 cut(s)	207, 455, 746
BcnI	CCSGG	8 cut(s)	550, 1129, 1243, 1715, 1832, 1833, 2554, 2728
BcoDI	GTCTC	6 cut(s)	32, 342, 1692, 1729, 1841, 2221
BcuI	ACTAGT	1 cut(s)	2330
BfaI	CTAG	7 cut(s)	1724, 2135, 2331, 2495, 2720, 2898, 3003
BfmI	CTRYAG	6 cut(s)	688, 959, 1588, 1840, 2320, 2848
BfuAI	ACCTGC	1 cut(s)	287
BmcAI	AGTACT	3 cut(s)	2363, 2709, 3021
Bme18I	GGWCC	2 cut(s)	393, 1058
BmeT110I	CYCGRG	5 cut(s)	665, 881, 1831, 2222, 2341
BmgBI	CACGTC	1 cut(s)	1108
BmgT120I	GGNCC	3 cut(s)	393, 1058, 1346
BmiI	GGNNCC	3 cut(s)	394, 1281, 1777
BmsI	GCATC	6 cut(s)	529, 702, 1736, 2208, 2254, 2931
BpiI	GAAGAC	1 cut(s)	1551
BpmI	CTGGAG	4 cut(s)	189, 536, 767, 839
Bpu10I	CCTNAGC	1 cut(s)	2268
Bpu14I	TTCGAA	4 cut(s)	317, 1331, 1439, 2246
BpuEI	CTTGAG	2 cut(s)	448, 2067
BpuMI	CCSGG	8 cut(s)	550, 1129, 1243, 1715, 1832, 1833, 2554, 2728
BsaAI	YACGTR	1 cut(s)	2400
BsaHI	GRCGYC	1 cut(s)	894
BsaI	GGTCTC	2 cut(s)	342, 1841
BsaJI	CCNNGG	6 cut(s)	560, 697, 1831, 1865, 2479, 2552
BsaXI	ACNNNNNCTCC	8 cut(s)	201, 231, 1247, 1253, 1277, 1283, 1685, 1715
Bsc4I	CCNNNNNNNGG	8 cut(s)	460, 810, 1248, 1249, 2222, 2560, 2654, 2753
Bse118I	RCCGGY	2 cut(s)	1055, 1772
Bse1I	ACTGG	4 cut(s)	519, 589, 1204, 2783
Bse21I	CCTNAGG	1 cut(s)	2841
BseAI	TCCGGA	1 cut(s)	1703
BseBI	CCWGG	3 cut(s)	207, 455, 746
BseDI	CCNNGG	6 cut(s)	560, 697, 1831, 1865, 2479, 2552
BseGI	GGATG	4 cut(s)	693, 2029, 2065, 2747
BseLI	CCNNNNNNNGG	8 cut(s)	460, 810, 1248, 1249, 2222, 2560, 2654, 2753
BseMII	CTCAG	7 cut(s)	891, 1082, 1612, 1724, 1938, 2259, 2832
BseNI	ACTGG	4 cut(s)	519, 589, 1204, 2783
BseRI	GAGGAG	2 cut(s)	117, 135
BseX3I	CGGCCG	2 cut(s)	783, 2188
BseYI	CCCAGC	1 cut(s)	626
BsgI	GTGCAG	4 cut(s)	389, 2294, 2378, 2459
Bsh1236I	CGCG	2 cut(s)	479, 2561
Bsh1285I	CGRYCG	2 cut(s)	786, 2191
BshFI	GGCC	4 cut(s)	785, 1348, 2190, 2846
BshNI	GGYRCC	1 cut(s)	1775
BshTI	ACCGGT	2 cut(s)	1055, 1772
BsiEI	CGRYCG	2 cut(s)	786, 2191
BsiHKAI	GWGCWC	4 cut(s)	825, 1874, 2210, 2288
BsiHKCI	CYCGRG	5 cut(s)	665, 881, 1831, 2222, 2341
BslFI	GGGAC	7 cut(s)	251, 493, 1144, 1174, 1526, 2438, 2771
BslI	CCNNNNNNNGG	8 cut(s)	460, 810, 1248, 1249, 2222, 2560, 2654, 2753
BsmAI	GTCTC	6 cut(s)	32, 342, 1692, 1729, 1841, 2221
BsmBI	CGTCTC	1 cut(s)	2221
BsmFI	GGGAC	7 cut(s)	251, 493, 1144, 1174, 1526, 2438, 2771
BsmI	GAATGC	1 cut(s)	38
BsnI	GGCC	4 cut(s)	785, 1348, 2190, 2846
Bso31I	GGTCTC	2 cut(s)	342, 1841
BsoBI	CYCGRG	5 cut(s)	665, 881, 1831, 2222, 2341
Bsp119I	TTCGAA	4 cut(s)	317, 1331, 1439, 2246
Bsp1286I	GDGCHC	6 cut(s)	825, 1658, 1874, 2210, 2288, 2574
Bsp13I	TCCGGA	1 cut(s)	1703
Bsp143I	GATC	7 cut(s)	622, 1750, 1990, 2179, 2386, 2556, 2743
Bsp19I	CCATGG	1 cut(s)	2479
BspACI	CCGC	7 cut(s)	157, 160, 246, 479, 1139, 2187, 2561
BspANI	GGCC	4 cut(s)	785, 1348, 2190, 2846
BspCNI	CTCAG	7 cut(s)	892, 1081, 1611, 1725, 1937, 2260, 2833
BspEI	TCCGGA	1 cut(s)	1703
BspFNI	CGCG	2 cut(s)	479, 2561
BspLI	GGNNCC	3 cut(s)	394, 1281, 1777
BspMAI	CTGCAG	4 cut(s)	692, 963, 1844, 2324
BspMI	ACCTGC	1 cut(s)	287
BspPI	GGATC	5 cut(s)	617, 2174, 2394, 2564, 2738
BspQI	GCTCTTC	1 cut(s)	2675
BspT104I	TTCGAA	4 cut(s)	317, 1331, 1439, 2246
BspT107I	GGYRCC	1 cut(s)	1775
BspTNI	GGTCTC	2 cut(s)	342, 1841
BsrFI	RCCGGY	2 cut(s)	1055, 1772
BsrI	ACTGG	4 cut(s)	519, 589, 1204, 2783
BssAI	RCCGGY	2 cut(s)	1055, 1772
BssECI	CCNNGG	6 cut(s)	560, 697, 1831, 1865, 2479, 2552
BssMI	GATC	7 cut(s)	622, 1750, 1990, 2179, 2386, 2556, 2743
BssNI	GRCGYC	1 cut(s)	894
BssSI	CACGAG	1 cut(s)	2957
BssT1I	CCWWGG	2 cut(s)	697, 2479
Bst2BI	CACGAG	1 cut(s)	2957
Bst2UI	CCWGG	3 cut(s)	207, 455, 746
Bst4CI	ACNGT	6 cut(s)	410, 437, 1043, 1869, 2000, 2636
Bst6I	CTCTTC	8 cut(s)	74, 135, 144, 345, 993, 2675, 2792, 2850
BstACI	GRCGYC	1 cut(s)	894
BstBAI	YACGTR	1 cut(s)	2400
BstBI	TTCGAA	4 cut(s)	317, 1331, 1439, 2246
BstC8I	GCNNGC	5 cut(s)	384, 1383, 1745, 1874, 2574
BstDSI	CCRYGG	3 cut(s)	560, 1865, 2479
BstEII	GGTNACC	1 cut(s)	1826
BstF5I	GGATG	4 cut(s)	693, 2029, 2065, 2747
BstFNI	CGCG	2 cut(s)	479, 2561
BstHHI	GCGC	1 cut(s)	1387
BstKTI	GATC	7 cut(s)	625, 1753, 1993, 2182, 2389, 2559, 2746
BstMAI	GTCTC	6 cut(s)	32, 342, 1692, 1729, 1841, 2221
BstMBI	GATC	7 cut(s)	622, 1750, 1990, 2179, 2386, 2556, 2743
BstMCI	CGRYCG	2 cut(s)	786, 2191
BstMWI	GCNNNNNNNGC	9 cut(s)	162, 670, 805, 814, 820, 1180, 1220, 2450, 2908
BstNI	CCWGG	3 cut(s)	207, 455, 746
BstNSI	RCATGY	3 cut(s)	1150, 1747, 2300
BstPI	GGTNACC	1 cut(s)	1826
BstSFI	CTRYAG	6 cut(s)	688, 959, 1588, 1840, 2320, 2848
BstUI	CGCG	2 cut(s)	479, 2561
BstV2I	GAAGAC	1 cut(s)	1551
BstX2I	RGATCY	1 cut(s)	2743
BstXI	CCANNNNNNTGG	3 cut(s)	1199, 1579, 2464
BstYI	RGATCY	1 cut(s)	2743
BstZI	CGGCCG	2 cut(s)	783, 2188
Bsu36I	CCTNAGG	1 cut(s)	2841
BsuRI	GGCC	4 cut(s)	785, 1348, 2190, 2846
BtgI	CCRYGG	3 cut(s)	560, 1865, 2479
BtgZI	GCGATG	1 cut(s)	2700
BtrI	CACGTC	1 cut(s)	1108
BtsCI	GGATG	4 cut(s)	693, 2029, 2065, 2747
BtsI	GCAGTG	1 cut(s)	2242
BtsIMutI	CAGTG	5 cut(s)	1606, 1932, 2059, 2175, 2242
BveI	ACCTGC	1 cut(s)	287
Cac8I	GCNNGC	5 cut(s)	384, 1383, 1745, 1874, 2574
CaiI	CAGNNNCTG	1 cut(s)	328
CfoI	GCGC	1 cut(s)	1387
Cfr10I	RCCGGY	2 cut(s)	1055, 1772
Cfr13I	GGNCC	3 cut(s)	393, 1058, 1346
Cfr9I	CCCGGG	1 cut(s)	1831
CseI	GACGC	2 cut(s)	902, 2435
CspAI	ACCGGT	2 cut(s)	1055, 1772
DpnI	GATC	7 cut(s)	624, 1752, 1992, 2181, 2388, 2558, 2745
DpnII	GATC	7 cut(s)	622, 1750, 1990, 2179, 2386, 2556, 2743
EaeI	YGGCCR	2 cut(s)	783, 2188
EagI	CGGCCG	2 cut(s)	783, 2188
Eam1104I	CTCTTC	8 cut(s)	74, 135, 144, 345, 993, 2675, 2792, 2850
EarI	CTCTTC	8 cut(s)	74, 135, 144, 345, 993, 2675, 2792, 2850
EciI	GGCGGA	1 cut(s)	1128
Ecl136II	GAGCTC	2 cut(s)	823, 2286
EclXI	CGGCCG	2 cut(s)	783, 2188
Eco130I	CCWWGG	2 cut(s)	697, 2479
Eco147I	AGGCCT	1 cut(s)	2846
Eco24I	GRGCYC	4 cut(s)	825, 1658, 2288, 2574
Eco31I	GGTCTC	2 cut(s)	342, 1841
Eco32I	GATATC	1 cut(s)	1363
Eco47I	GGWCC	2 cut(s)	393, 1058
Eco52I	CGGCCG	2 cut(s)	783, 2188
Eco53kI	GAGCTC	2 cut(s)	823, 2286
Eco57I	CTGAAG	3 cut(s)	1169, 2035, 2816
Eco72I	CACGTG	1 cut(s)	2400
Eco81I	CCTNAGG	1 cut(s)	2841
Eco88I	CYCGRG	5 cut(s)	665, 881, 1831, 2222, 2341
Eco91I	GGTNACC	1 cut(s)	1826
EcoICRI	GAGCTC	2 cut(s)	823, 2286
EcoO65I	GGTNACC	1 cut(s)	1826
EcoRI	GAATTC	1 cut(s)	1672
EcoRII	CCWGG	3 cut(s)	205, 453, 744
EcoRV	GATATC	1 cut(s)	1363
EcoT14I	CCWWGG	2 cut(s)	697, 2479
EcoT38I	GRGCYC	4 cut(s)	825, 1658, 2288, 2574
ErhI	CCWWGG	2 cut(s)	697, 2479
Esp3I	CGTCTC	1 cut(s)	2221
FaqI	GGGAC	7 cut(s)	251, 493, 1144, 1174, 1526, 2438, 2771
FauI	CCCGC	1 cut(s)	472
FauNDI	CATATG	2 cut(s)	356, 2168
FblI	GTMKAC	2 cut(s)	2121, 2355
FokI	GGATG	4 cut(s)	680, 2036, 2072, 2734
FriOI	GRGCYC	4 cut(s)	825, 1658, 2288, 2574
FspBI	CTAG	7 cut(s)	1724, 2135, 2331, 2495, 2720, 2898, 3003
FspI	TGCGCA	1 cut(s)	1386
GlaI	GCGC	1 cut(s)	1386
GsaI	CCCAGC	1 cut(s)	630
GsuI	CTGGAG	4 cut(s)	189, 536, 767, 839
HaeIII	GGCC	4 cut(s)	785, 1348, 2190, 2846
HgaI	GACGC	2 cut(s)	902, 2435
HhaI	GCGC	1 cut(s)	1387
Hin1I	GRCGYC	1 cut(s)	894
Hin6I	GCGC	1 cut(s)	1385
HinP1I	GCGC	1 cut(s)	1385
HincII	GTYRAC	4 cut(s)	80, 1239, 1255, 1446
HindII	GTYRAC	4 cut(s)	80, 1239, 1255, 1446
HindIII	AAGCTT	5 cut(s)	848, 1019, 1379, 2377, 2414
Hpy99I	CGWCG	7 cut(s)	171, 290, 293, 896, 1109, 1237, 1898
HpyAV	CCTTC	8 cut(s)	235, 908, 1337, 1975, 2002, 2059, 2248, 2928
HpyCH4III	ACNGT	6 cut(s)	410, 437, 1043, 1869, 2000, 2636
HpyCH4IV	ACGT	5 cut(s)	285, 1107, 1235, 2279, 2399
HpyF10VI	GCNNNNNNNGC	9 cut(s)	162, 670, 805, 814, 820, 1180, 1220, 2450, 2908
HpySE526I	ACGT	5 cut(s)	285, 1107, 1235, 2279, 2399
Hsp92I	GRCGYC	1 cut(s)	894
HspAI	GCGC	1 cut(s)	1385
Kpn2I	TCCGGA	1 cut(s)	1703
Kzo9I	GATC	7 cut(s)	622, 1750, 1990, 2179, 2386, 2556, 2743
LguI	GCTCTTC	1 cut(s)	2675
LmnI	GCTCC	5 cut(s)	517, 820, 2450, 2650, 2716
LweI	GCATC	6 cut(s)	529, 702, 1736, 2208, 2254, 2931
MaeI	CTAG	7 cut(s)	1724, 2135, 2331, 2495, 2720, 2898, 3003
MaeII	ACGT	5 cut(s)	285, 1107, 1235, 2279, 2399
MaeIII	GTNAC	9 cut(s)	608, 1043, 1601, 1826, 2130, 2522, 2636, 2657, 2722
MalI	GATC	7 cut(s)	624, 1752, 1992, 2181, 2388, 2558, 2745
MboI	GATC	7 cut(s)	622, 1750, 1990, 2179, 2386, 2556, 2743
MfeI	CAATTG	1 cut(s)	1523
MflI	RGATCY	1 cut(s)	2743
MhlI	GDGCHC	6 cut(s)	825, 1658, 1874, 2210, 2288, 2574
MlyI	GAGTC	8 cut(s)	34, 204, 233, 344, 2333, 2600, 2605, 2822
MmeI	TCCRAC	7 cut(s)	175, 327, 1068, 1092, 1917, 2428, 2445
MroI	TCCGGA	1 cut(s)	1703
MroXI	GAANNNNTTC	2 cut(s)	2198, 2315
MseI	TTAA	7 cut(s)	6, 657, 1013, 1196, 1973, 2601, 3024
MslI	CAYNNNNRTG	7 cut(s)	19, 578, 1106, 2301, 2400, 2484, 2508
MspA1I	CMGCKG	4 cut(s)	540, 817, 964, 1174
MunI	CAATTG	1 cut(s)	1523
Mva1269I	GAATGC	1 cut(s)	38
MvaI	CCWGG	3 cut(s)	207, 455, 746
MvnI	CGCG	2 cut(s)	479, 2561
MwoI	GCNNNNNNNGC	9 cut(s)	162, 670, 805, 814, 820, 1180, 1220, 2450, 2908
NciI	CCSGG	8 cut(s)	550, 1129, 1243, 1715, 1832, 1833, 2554, 2728
NcoI	CCATGG	1 cut(s)	2479
NdeI	CATATG	2 cut(s)	356, 2168
NdeII	GATC	7 cut(s)	622, 1750, 1990, 2179, 2386, 2556, 2743
NlaIV	GGNNCC	3 cut(s)	394, 1281, 1777
NmeAIII	GCCGAG	1 cut(s)	811
NmuCI	GTSAC	4 cut(s)	608, 1601, 2130, 2722
NsbI	TGCGCA	1 cut(s)	1386
NspI	RCATGY	3 cut(s)	1150, 1747, 2300
NspV	TTCGAA	4 cut(s)	317, 1331, 1439, 2246
OliI	CACNNNNGTG	1 cut(s)	2400
PaeI	GCATGC	1 cut(s)	1747
PaeR7I	CTCGAG	3 cut(s)	665, 881, 2341
PaqCI	CACCTGC	1 cut(s)	287
PceI	AGGCCT	1 cut(s)	2846
PciI	ACATGT	1 cut(s)	1146
PciSI	GCTCTTC	1 cut(s)	2675
PcsI	WCGNNNNNNNCGW	1 cut(s)	888
PctI	GAATGC	1 cut(s)	38
PdmI	GAANNNNTTC	2 cut(s)	2198, 2315
PflFI	GACNNNGTC	1 cut(s)	1898
PflMI	CCANNNNNTGG	2 cut(s)	810, 2753
PfoI	TCCNGGA	3 cut(s)	205, 548, 1713
PinAI	ACCGGT	2 cut(s)	1055, 1772
PleI	GAGTC	8 cut(s)	33, 204, 233, 344, 2333, 2600, 2605, 2822
PmaCI	CACGTG	1 cut(s)	2400
PmlI	CACGTG	1 cut(s)	2400
PpsI	GAGTC	8 cut(s)	33, 204, 233, 344, 2333, 2600, 2605, 2822
Ppu21I	YACGTR	1 cut(s)	2400
PscI	ACATGT	1 cut(s)	1146
Psp124BI	GAGCTC	2 cut(s)	825, 2288
Psp6I	CCWGG	3 cut(s)	205, 453, 744
PspCI	CACGTG	1 cut(s)	2400
PspEI	GGTNACC	1 cut(s)	1826
PspFI	CCCAGC	1 cut(s)	626
PspGI	CCWGG	3 cut(s)	205, 453, 744
PspN4I	GGNNCC	3 cut(s)	394, 1281, 1777
PspPI	GGNCC	3 cut(s)	393, 1058, 1346
PspXI	VCTCGAGB	2 cut(s)	665, 2341
PstI	CTGCAG	4 cut(s)	692, 963, 1844, 2324
PstNI	CAGNNNCTG	1 cut(s)	328
PsuI	RGATCY	1 cut(s)	2743
PsyI	GACNNNGTC	1 cut(s)	1898
PvuII	CAGCTG	4 cut(s)	540, 817, 964, 1174
RseI	CAYNNNNRTG	7 cut(s)	19, 578, 1106, 2301, 2400, 2484, 2508
SacI	GAGCTC	2 cut(s)	825, 2288
SapI	GCTCTTC	1 cut(s)	2675
SaqAI	TTAA	7 cut(s)	6, 657, 1013, 1196, 1973, 2601, 3024
Sau3AI	GATC	7 cut(s)	622, 1750, 1990, 2179, 2386, 2556, 2743
Sau96I	GGNCC	3 cut(s)	393, 1058, 1346
ScaI	AGTACT	3 cut(s)	2363, 2709, 3021
SchI	GAGTC	8 cut(s)	34, 204, 233, 344, 2333, 2600, 2605, 2822
SduI	GDGCHC	6 cut(s)	825, 1658, 1874, 2210, 2288, 2574
SfaNI	GCATC	6 cut(s)	529, 702, 1736, 2208, 2254, 2931
SfcI	CTRYAG	6 cut(s)	688, 959, 1588, 1840, 2320, 2848
Sfr274I	CTCGAG	3 cut(s)	665, 881, 2341
SfuI	TTCGAA	4 cut(s)	317, 1331, 1439, 2246
SinI	GGWCC	2 cut(s)	393, 1058
SlaI	CTCGAG	3 cut(s)	665, 881, 2341
SmaI	CCCGGG	1 cut(s)	1833
SmiMI	CAYNNNNRTG	7 cut(s)	19, 578, 1106, 2301, 2400, 2484, 2508
SmlI	CTYRAG	5 cut(s)	427, 665, 881, 2082, 2341
SmoI	CTYRAG	5 cut(s)	427, 665, 881, 2082, 2341
SpeI	ACTAGT	1 cut(s)	2330
SphI	GCATGC	1 cut(s)	1747
SseBI	AGGCCT	1 cut(s)	2846
SsiI	CCGC	7 cut(s)	157, 160, 246, 479, 1139, 2187, 2561
SspI	AATATT	1 cut(s)	1941
SspMI	CTAG	7 cut(s)	1724, 2135, 2331, 2495, 2720, 2898, 3003
SstI	GAGCTC	2 cut(s)	825, 2288
StuI	AGGCCT	1 cut(s)	2846
StyI	CCWWGG	2 cut(s)	697, 2479
TaaI	ACNGT	6 cut(s)	410, 437, 1043, 1869, 2000, 2636
TaiI	ACGT	5 cut(s)	288, 1110, 1238, 2282, 2402
TatI	WGTACW	3 cut(s)	2361, 2707, 3019
TauI	GCSGC	3 cut(s)	159, 162, 2190
Tru1I	TTAA	7 cut(s)	6, 657, 1013, 1196, 1973, 2601, 3024
Tru9I	TTAA	7 cut(s)	6, 657, 1013, 1196, 1973, 2601, 3024
TscAI	CASTG	5 cut(s)	1606, 1932, 2059, 2182, 2242
TseFI	GTSAC	4 cut(s)	608, 1601, 2130, 2722
Tsp45I	GTSAC	4 cut(s)	608, 1601, 2130, 2722
TspDTI	ATGAA	8 cut(s)	474, 515, 1090, 1181, 1992, 2426, 2492, 2981
TspGWI	ACGGA	3 cut(s)	333, 434, 2388
TspMI	CCCGGG	1 cut(s)	1831
TspRI	CASTG	5 cut(s)	1606, 1932, 2059, 2182, 2242
Tth111I	GACNNNGTC	1 cut(s)	1898
Van91I	CCANNNNNTGG	2 cut(s)	810, 2753
VpaK11BI	GGWCC	2 cut(s)	393, 1058
XapI	RAATTY	6 cut(s)	263, 777, 904, 1206, 1672, 2258
XceI	RCATGY	3 cut(s)	1150, 1747, 2300
XhoI	CTCGAG	3 cut(s)	665, 881, 2341
XmaI	CCCGGG	1 cut(s)	1831
XmiI	GTMKAC	2 cut(s)	2121, 2355
XmnI	GAANNNNTTC	2 cut(s)	2198, 2315
XspI	CTAG	7 cut(s)	1724, 2135, 2331, 2495, 2720, 2898, 3003
ZrmI	AGTACT	3 cut(s)	2363, 2709, 3021

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.