Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0004091

Physical Location & Seq

Genomic Coordinates Reverse (-)

35146911 .. 35149949

Gene Structure 3039 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000002489

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2916 bp

ATGGAGTCTCACTTACGCATTCCCATCCTCAAAGATCATCGATCCATCCACTCTTCGTCTGTCAACTCCATCATGTCATTACCCAAAAACCACCACCTCCATCTCTACCTCCTCTTCTTCCTCTTCCTCCTCTCCAACGCCGCCGCCGCAGCCGATGAGCTTCAAATCCTACTAAAACTCAAATCCTCCCTCCAAGACTCCAACGCCCAACTCTTCGCCACATGGGATTCCACCGCCAATTCGTTTTGCACCTTCACCGGAATTACCTGCAACAACGTCGGTTCGGTTCGAGAAATCGAACTTTCAAACCAGAAGCTGTCGGGGTCTCTTCCGTTGGACTCGATATGCCAACTTCCATCATTAGAAAAGCTCGCTTTCGGGTCCAATTTCCTGCACGGGACAATCATGGAGGACTTGAGAAACTGTACGAATTTGAAGTACCTGGATTTGGCGAAAAACTTGTTCACGGGTTCATTCCCTGACATATCTTCCTTGTCCCAACTGGAGCATCTTCATTTAAACGACAGCGTGTTTTCGGGGATTTTCCCGTGGACTTCCCTCACCAACATGACTGGTCTGATTCGTTTGAGCTTGGGTGACAACCCGTTTGATCCGAGCCCGTTTCCAAAGGAAGTAGTAACTCTTAAGAAGCTCGAGTGGCTCTTCTTGGGATTCTGCAGCATCCAAGGAACGATACCGAGTGAAATCGGGAACTTGGTCGAGCTTATCAACCTGGAGCTGTCCGGTAACAACATGACCGGAGAAATTCCGGCTGAGATTGGACAGCTCACCAAGCTCTGGCAGCTGGAGCTCTGGGCCAACAGTTTCACCGGAAAACTTCCTTTCGGGCTAAGAAACCTCACCAATCTCGAGAACTTCGACGCTTCGGCGAATTTTCTAGAAGGCGATTTGAACGAGTTGGGGTTTCTGACCAAGTTGGTTTCTCTGCAGCTGTCTAAGAACAATTTTTCAGGCGAAGTACCGGAAGAATTCAGCGAGTTCAAGAAGCTTGTGAATCTGTCTTTGTACGCTAACAAGCTGACCGGTCCTCTGCCTCAGAAATTGGGGTCTTGGTCGGAGATGGACTTCATCGATGTGTCGGAGAATTTCTTTACCGGGACTATTCCGCCGGACATGTGCAACAAAGGGACGATGAAGGAGCTGCTCATGCGTCAGAACAAGTTGACCGGTCAAATTCCGGCAAACTATGCGAAGTGCACGACGCTGACCCGGTTCAGGGTCAACAACAACTCCCTCTCCGGTGTGGTTCCGGCTGGACTTTTGGGATTGCCGAATGTGGCAATCATTGACATCACTTCGAATCAACTTGTAGGTCCGATTACTTCCGATATCGGAAATGCCAAGAAGCTTGCGCGGTTGTTTGTGAGTTACAATCGGTTATCTGGTGAATTACCGGACGAGCTTTCGAAGTCAACTTCGTTGGTTTCGGTTGTGTTGAATAACAATCAGTTTTCCGGTAAGATTCCAGCGAAGATAGGGGACTTGAAGCAATTGAGTACTCTGTATTTACAGAGCAACTTGTTGTCTTCTTCAATTCCCAAGTCATTAGGAAGCTGTAGTTTCCTGAGGGACTTGAACATGGCAAACAACTCGCTTTCCGGTAAAATCCCGTCATGTTTGGGCTCTTTGCTGAACTTGAATTCTTTGGATTTGTCTCACAATCAACTCTCCGGTAAAATCCCGAAAAGTCTGGCATCTCTGAGACTACGGATGCTTGATCTTTCGCACAACAGACTGACCGGTGCCGTACCAGAATCTCTCTCGATTGCAGCCTATAACGGCAGCCTTCGCAGTAACCCGGGTCTCTGCAGCATGGACATCACCTACTTCCCACTGACCCTTATTTGCTTCTCAGTGGGTATAGCAATACTGTTTGTTTCACTCATTTGCTTCTTGTTCTTAAGGAGGAAGAAGAAAGATCAAGACCGTTCATTGAAGGAAGAATCTTGGGATGTGAAGTCTTTCCATGTAATAACCTTCAGTGAGGATGAGATTCTTGATTCCATTACCCAAGAGAATCTAATTGGAAAAGGAGGTTCTGGAAATGTCTACAAAGTGTCACTAGCCAACGGCAAAGAACTCGCCGTGAAGCACATATGGAACACTGATCCAAGCGGCCGGAAAATGTTCAAGAGCACCACACCCATGCTCGGGAGACGGGGCAGTGGTAGTTCAAAATCGAAGGAATTTGATGCTGAGCTGCAGACGTTGAGCTCAGTAAGGCATGTGAATGTGGTGAAGTTGTTCTGCAGCATTACTAGTGAGGACTCAAGCCTTTTGGTCTACGAGTACTTACCGAATGGAAGCTTGTGGGATCGGCTGCACATGTGTGAGAACATGAAGCTTGATTGGGACGCAAGGTATGAGATTGCAGTCGGAGCAGCCAAAGGGTTGAAGTATCTGCACCATGGCTGTGAGAGGCTAGTGATTCATAGAGATGTCAAGTCCAGTAACATTTTGTTAGACGAGTTTTTGAAGCCCCGGATTGCGGATTTTGGGCTTGCCAAGATGGTTCAGCCTAATGCAATCAAGGACTCGACTCATGTTGTTGCCGGAACACACGGTTACATAGCTCCTGAATATGGTTACACCTACAAGGTGAATGAGAAGAGCGATGTGTATAGCTTCGGTGTAGTACTAATGGAGCTAGTGACCGGGAAAAAACCGATAGATCCATCATTTGGGGACAACAAGGACATAGTGAACTGGATATGTGGCAACCTGAAGAGTAGGGAGAGTGTGTTGGATGTGGTGGACTCATACATTCCTGAGGCATATAGAGAAGAGGCTATCAAGGTACTGAGAATTGCAGTTCTATGCACAGCTAGGCTTCCAGAGCTAAGACCTTCCATGAGAAGTGTCGTTCAAATGCTTGAAGAAGCTCGGAGCCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001653 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009791 GO:0009845 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045087 GO:0046777 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0090351 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

972

Amino Acids

107.49

Weight (kDa)

7.04

Isoelectric Point (pI)

32.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	51 - 91	3.4e-09	Leucine rich repeat N-terminal domain
LRR_14	PF23598	214 - 333	3.4e-11	Leucine-rich repeat region
LRR_14	PF23598	447 - 591	2.3e-06	Leucine-rich repeat region
LRR_8	PF13855	529 - 586	9.2e-07	Leucine rich repeat
Pkinase	PF00069	678 - 963	5.4e-40	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	679 - 965	1.1e-36	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0004091)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09970 AT1G09970
fragaria_vesca	FvH4_5g33590
malus_domestica	MD08G1210000.v1.1 MD15G1394100.v1.1
prunus_persica	Prupe.1G542300_v2.0.a1
pyrus_communis	pycom08g18120 pycom15g35400 pycom15g35410
rosa_chinensis	RchiOBHm_Chr7g0217331 RchiOBHm_Chr7g0232471
rosa_laevigata	RLG00000001335 RLG00000002489 RLG00000002493
rosa_multiflora	Rmu_sc0001898.1_g000015 Rmu_sc0004663.1_g000011 Rmu_sc0011824.1_g000016
rosa_roxburghii	Rroxscaffold_3G00228280 Rroxscaffold_3G00242430
rosa_rugosa	Rorug07G0168100 Rorug07G0274700
rosa_samantha	Rh7AG307400 Rh7AG307800 Rh7AG428100 Rh7BG298200 Rh7BG402600 Rh7CG326500 Rh7CG326900 Rh7CG447100 Rh7DG306900 Rh7DG418900
rosa_wichuraiana	Rw7G026090 Rw7G035470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	275
AccB1I	GGYRCC	1 cut(s)	1763
AccB7I	CCANNNNNTGG	2 cut(s)	798, 2702
AccI	GTMKAC	2 cut(s)	2070, 2304
AccII	CGCG	1 cut(s)	1375
AciI	CCGC	8 cut(s)	141, 144, 147, 234, 1127, 1375, 2136, 2510
AclWI	GGATC	5 cut(s)	36, 605, 2123, 2343, 2687
AcoI	YGGCCR	1 cut(s)	2137
AcsI	RAATTY	8 cut(s)	430, 765, 892, 989, 1105, 1194, 1660, 2207
AcuI	CTGAAG	2 cut(s)	1984, 2765
AfaI	GTAC	9 cut(s)	427, 440, 981, 1028, 1519, 1770, 2312, 2658, 2820
AfiI	CCNNNNNNNGG	9 cut(s)	448, 798, 1236, 1237, 2171, 2172, 2509, 2603, 2702
AflII	CTTAAG	2 cut(s)	644, 1921
AflIII	ACRYGT	2 cut(s)	1134, 2346
AgeI	ACCGGT	3 cut(s)	1043, 1187, 1760
AhlI	ACTAGT	1 cut(s)	2279
AjnI	CCWGG	2 cut(s)	441, 732
AleI	CACNNNNGTG	1 cut(s)	2349
Alw21I	GWGCWC	4 cut(s)	813, 1220, 2159, 2237
Alw26I	GTCTC	6 cut(s)	12, 330, 1680, 1717, 1829, 2170
Alw44I	GTGCAC	1 cut(s)	1216
AlwI	GGATC	5 cut(s)	36, 605, 2123, 2343, 2687
AlwNI	CAGNNNCTG	1 cut(s)	316
Ama87I	CYCGRG	4 cut(s)	653, 869, 1819, 2171
AoxI	GGCC	2 cut(s)	816, 2137
ApaLI	GTGCAC	1 cut(s)	1216
ApoI	RAATTY	8 cut(s)	430, 765, 892, 989, 1105, 1194, 1660, 2207
ArsI	GACNNNNNNTTYG	2 cut(s)	590, 622
AsiGI	ACCGGT	3 cut(s)	1043, 1187, 1760
Asp700I	GAANNNNTTC	3 cut(s)	1982, 2147, 2264
AspLEI	GCGC	1 cut(s)	1375
AspS9I	GGNCC	4 cut(s)	381, 816, 1046, 1334
AsuC2I	CCSGG	6 cut(s)	1117, 1231, 1820, 1821, 2503, 2677
AsuII	TTCGAA	2 cut(s)	1319, 1427
AvaI	CYCGRG	4 cut(s)	653, 869, 1819, 2171
AvaII	GGWCC	3 cut(s)	381, 1046, 1334
AxyI	CCTNAGG	2 cut(s)	1586, 2790
BaeGI	GKGCMC	1 cut(s)	1220
BanI	GGYRCC	1 cut(s)	1763
BanII	GRGCYC	4 cut(s)	620, 813, 1646, 2237
BbsI	GAAGAC	1 cut(s)	1539
Bbv12I	GWGCWC	4 cut(s)	813, 1220, 2159, 2237
BccI	CCATC	8 cut(s)	32, 53, 77, 108, 364, 1075, 2524, 2704
BceAI	ACGGC	4 cut(s)	1751, 1816, 2090, 2107
BcgI	CGANNNNNNTGC	6 cut(s)	259, 293, 1593, 1627, 2083, 2117
BciT130I	CCWGG	2 cut(s)	443, 734
BcnI	CCSGG	6 cut(s)	1117, 1231, 1820, 1821, 2503, 2677
BcoDI	GTCTC	6 cut(s)	12, 330, 1680, 1717, 1829, 2170
BcuI	ACTAGT	1 cut(s)	2279
BfaI	CTAG	6 cut(s)	899, 2084, 2280, 2444, 2669, 2847
BfmI	CTRYAG	6 cut(s)	676, 947, 1576, 1828, 2222, 2269
BfrI	CTTAAG	2 cut(s)	644, 1921
BfuAI	ACCTGC	1 cut(s)	275
BlpI	GCTNAGC	1 cut(s)	2217
BmcAI	AGTACT	3 cut(s)	1519, 2312, 2658
Bme1390I	CCNGG	8 cut(s)	443, 734, 1117, 1231, 1820, 1821, 2503, 2677
Bme18I	GGWCC	3 cut(s)	381, 1046, 1334
BmeT110I	CYCGRG	4 cut(s)	653, 869, 1819, 2171
BmgT120I	GGNCC	4 cut(s)	381, 816, 1046, 1334
BmiI	GGNNCC	4 cut(s)	382, 1269, 1765, 2909
BmrFI	CCNGG	8 cut(s)	443, 734, 1117, 1231, 1820, 1821, 2503, 2677
BmsI	GCATC	5 cut(s)	517, 690, 1722, 1724, 2203
BpiI	GAAGAC	1 cut(s)	1539
BpmI	CTGGAG	3 cut(s)	524, 755, 827
Bpu1102I	GCTNAGC	1 cut(s)	2217
Bpu14I	TTCGAA	2 cut(s)	1319, 1427
BpuEI	CTTGAG	2 cut(s)	436, 2275
BpuMI	CCSGG	6 cut(s)	1117, 1231, 1820, 1821, 2503, 2677
Bsa29I	ATCGAT	2 cut(s)	40, 1092
BsaI	GGTCTC	2 cut(s)	330, 1829
BsaJI	CCNNGG	5 cut(s)	548, 685, 1819, 2428, 2501
BsaXI	ACNNNNNCTCC	6 cut(s)	1235, 1241, 1265, 1271, 2747, 2777
Bsc4I	CCNNNNNNNGG	9 cut(s)	448, 798, 1236, 1237, 2171, 2172, 2509, 2603, 2702
Bse118I	RCCGGY	3 cut(s)	1043, 1187, 1760
Bse1I	ACTGG	4 cut(s)	507, 577, 2469, 2732
Bse21I	CCTNAGG	2 cut(s)	1586, 2790
BseBI	CCWGG	2 cut(s)	443, 734
BseCI	ATCGAT	2 cut(s)	40, 1092
BseDI	CCNNGG	5 cut(s)	548, 685, 1819, 2428, 2501
BseGI	GGATG	7 cut(s)	24, 45, 681, 1737, 1978, 2014, 2773
BseLI	CCNNNNNNNGG	9 cut(s)	448, 798, 1236, 1237, 2171, 2172, 2509, 2603, 2702
BseMII	CTCAG	9 cut(s)	765, 1070, 1577, 1712, 1887, 2208, 2250, 2781, 2813
BseNI	ACTGG	4 cut(s)	507, 577, 2469, 2732
BseRI	GAGGAG	2 cut(s)	101, 119
BseSI	GKGCMC	1 cut(s)	1220
BseX3I	CGGCCG	1 cut(s)	2137
BsgI	GTGCAG	3 cut(s)	377, 2327, 2408
Bsh1236I	CGCG	1 cut(s)	1375
Bsh1285I	CGRYCG	1 cut(s)	2140
BshFI	GGCC	2 cut(s)	818, 2139
BshNI	GGYRCC	1 cut(s)	1763
BshTI	ACCGGT	3 cut(s)	1043, 1187, 1760
BshVI	ATCGAT	2 cut(s)	40, 1092
BsiEI	CGRYCG	1 cut(s)	2140
BsiHKAI	GWGCWC	4 cut(s)	813, 1220, 2159, 2237
BsiHKCI	CYCGRG	4 cut(s)	653, 869, 1819, 2171
BslFI	GGGAC	8 cut(s)	412, 481, 1132, 1162, 1514, 1604, 2387, 2720
BslI	CCNNNNNNNGG	9 cut(s)	448, 798, 1236, 1237, 2171, 2172, 2509, 2603, 2702
BsmAI	GTCTC	6 cut(s)	12, 330, 1680, 1717, 1829, 2170
BsmBI	CGTCTC	1 cut(s)	2170
BsmFI	GGGAC	8 cut(s)	412, 481, 1132, 1162, 1514, 1604, 2387, 2720
BsmI	GAATGC	1 cut(s)	18
BsnI	GGCC	2 cut(s)	818, 2139
Bso31I	GGTCTC	2 cut(s)	330, 1829
BsoBI	CYCGRG	4 cut(s)	653, 869, 1819, 2171
Bsp119I	TTCGAA	2 cut(s)	1319, 1427
Bsp1286I	GDGCHC	6 cut(s)	620, 813, 1220, 1646, 2159, 2237
Bsp143I	GATC	8 cut(s)	34, 41, 610, 1738, 1939, 2128, 2335, 2692
Bsp1720I	GCTNAGC	1 cut(s)	2217
Bsp19I	CCATGG	1 cut(s)	2428
BspACI	CCGC	8 cut(s)	141, 144, 147, 234, 1127, 1375, 2136, 2510
BspANI	GGCC	2 cut(s)	818, 2139
BspCNI	CTCAG	9 cut(s)	766, 1069, 1578, 1713, 1886, 2209, 2249, 2782, 2814
BspDI	ATCGAT	2 cut(s)	40, 1092
BspFNI	CGCG	1 cut(s)	1375
BspLI	GGNNCC	4 cut(s)	382, 1269, 1765, 2909
BspMAI	CTGCAG	5 cut(s)	680, 951, 1832, 2226, 2273
BspMI	ACCTGC	1 cut(s)	275
BspPI	GGATC	5 cut(s)	36, 605, 2123, 2343, 2687
BspQI	GCTCTTC	2 cut(s)	668, 2624
BspT104I	TTCGAA	2 cut(s)	1319, 1427
BspT107I	GGYRCC	1 cut(s)	1763
BspTI	CTTAAG	2 cut(s)	644, 1921
BspTNI	GGTCTC	2 cut(s)	330, 1829
BsrFI	RCCGGY	3 cut(s)	1043, 1187, 1760
BsrI	ACTGG	4 cut(s)	507, 577, 2469, 2732
BssAI	RCCGGY	3 cut(s)	1043, 1187, 1760
BssECI	CCNNGG	5 cut(s)	548, 685, 1819, 2428, 2501
BssMI	GATC	8 cut(s)	34, 41, 610, 1738, 1939, 2128, 2335, 2692
BssT1I	CCWWGG	2 cut(s)	685, 2428
Bst2UI	CCWGG	2 cut(s)	443, 734
Bst4CI	ACNGT	5 cut(s)	425, 824, 1893, 1949, 2585
Bst6I	CTCTTC	9 cut(s)	58, 119, 128, 218, 333, 668, 2624, 2741, 2799
BstAFI	CTTAAG	2 cut(s)	644, 1921
BstAPI	GCANNNNNTGC	1 cut(s)	1208
BstBI	TTCGAA	2 cut(s)	1319, 1427
BstC8I	GCNNGC	3 cut(s)	372, 1371, 2523
BstDSI	CCRYGG	2 cut(s)	548, 2428
BstF5I	GGATG	7 cut(s)	24, 45, 681, 1737, 1978, 2014, 2773
BstFNI	CGCG	1 cut(s)	1375
BstHHI	GCGC	1 cut(s)	1375
BstKTI	GATC	8 cut(s)	37, 44, 613, 1741, 1942, 2131, 2338, 2695
BstMAI	GTCTC	6 cut(s)	12, 330, 1680, 1717, 1829, 2170
BstMBI	GATC	8 cut(s)	34, 41, 610, 1738, 1939, 2128, 2335, 2692
BstMCI	CGRYCG	1 cut(s)	2140
BstNI	CCWGG	2 cut(s)	443, 734
BstNSI	RCATGY	3 cut(s)	1138, 2249, 2350
BstSCI	CCNGG	8 cut(s)	441, 732, 1115, 1229, 1818, 1819, 2501, 2675
BstSFI	CTRYAG	6 cut(s)	676, 947, 1576, 1828, 2222, 2269
BstSLI	GKGCMC	1 cut(s)	1220
BstUI	CGCG	1 cut(s)	1375
BstV2I	GAAGAC	1 cut(s)	1539
BstX2I	RGATCY	1 cut(s)	2692
BstYI	RGATCY	1 cut(s)	2692
BstZI	CGGCCG	1 cut(s)	2137
Bsu15I	ATCGAT	2 cut(s)	40, 1092
Bsu36I	CCTNAGG	2 cut(s)	1586, 2790
BsuRI	GGCC	2 cut(s)	818, 2139
BsuTUI	ATCGAT	2 cut(s)	40, 1092
BtgI	CCRYGG	2 cut(s)	548, 2428
BtgZI	GCGATG	1 cut(s)	2649
BtsCI	GGATG	7 cut(s)	24, 45, 681, 1737, 1978, 2014, 2773
BtsI	GCAGTG	1 cut(s)	2191
BtsIMutI	CAGTG	5 cut(s)	1853, 1881, 2008, 2124, 2191
BveI	ACCTGC	1 cut(s)	275
Cac8I	GCNNGC	3 cut(s)	372, 1371, 2523
CaiI	CAGNNNCTG	1 cut(s)	316
CfoI	GCGC	1 cut(s)	1375
Cfr10I	RCCGGY	3 cut(s)	1043, 1187, 1760
Cfr13I	GGNCC	4 cut(s)	381, 816, 1046, 1334
Cfr9I	CCCGGG	1 cut(s)	1819
ClaI	ATCGAT	2 cut(s)	40, 1092
CseI	GACGC	4 cut(s)	890, 1160, 1231, 2384
Csp6I	GTAC	9 cut(s)	426, 439, 980, 1027, 1518, 1769, 2311, 2657, 2819
CspAI	ACCGGT	3 cut(s)	1043, 1187, 1760
CviQI	GTAC	9 cut(s)	426, 439, 980, 1027, 1518, 1769, 2311, 2657, 2819
DpnI	GATC	8 cut(s)	36, 43, 612, 1740, 1941, 2130, 2337, 2694
DpnII	GATC	8 cut(s)	34, 41, 610, 1738, 1939, 2128, 2335, 2692
DraI	TTTAAA	1 cut(s)	519
EaeI	YGGCCR	1 cut(s)	2137
EagI	CGGCCG	1 cut(s)	2137
Eam1104I	CTCTTC	9 cut(s)	58, 119, 128, 218, 333, 668, 2624, 2741, 2799
EarI	CTCTTC	9 cut(s)	58, 119, 128, 218, 333, 668, 2624, 2741, 2799
EciI	GGCGGA	1 cut(s)	1116
Ecl136II	GAGCTC	2 cut(s)	811, 2235
EclXI	CGGCCG	1 cut(s)	2137
Eco130I	CCWWGG	2 cut(s)	685, 2428
Eco24I	GRGCYC	4 cut(s)	620, 813, 1646, 2237
Eco31I	GGTCTC	2 cut(s)	330, 1829
Eco32I	GATATC	1 cut(s)	1351
Eco47I	GGWCC	3 cut(s)	381, 1046, 1334
Eco52I	CGGCCG	1 cut(s)	2137
Eco53kI	GAGCTC	2 cut(s)	811, 2235
Eco57I	CTGAAG	2 cut(s)	1984, 2765
Eco81I	CCTNAGG	2 cut(s)	1586, 2790
Eco88I	CYCGRG	4 cut(s)	653, 869, 1819, 2171
EcoICRI	GAGCTC	2 cut(s)	811, 2235
EcoRI	GAATTC	2 cut(s)	989, 1660
EcoRII	CCWGG	2 cut(s)	441, 732
EcoRV	GATATC	1 cut(s)	1351
EcoT14I	CCWWGG	2 cut(s)	685, 2428
EcoT38I	GRGCYC	4 cut(s)	620, 813, 1646, 2237
ErhI	CCWWGG	2 cut(s)	685, 2428
Esp3I	CGTCTC	1 cut(s)	2170
FaqI	GGGAC	8 cut(s)	412, 481, 1132, 1162, 1514, 1604, 2387, 2720
FauNDI	CATATG	1 cut(s)	2117
FblI	GTMKAC	2 cut(s)	2070, 2304
FokI	GGATG	7 cut(s)	11, 32, 668, 1744, 1985, 2021, 2780
FriOI	GRGCYC	4 cut(s)	620, 813, 1646, 2237
FspBI	CTAG	6 cut(s)	899, 2084, 2280, 2444, 2669, 2847
GlaI	GCGC	1 cut(s)	1374
GsuI	CTGGAG	3 cut(s)	524, 755, 827
HaeIII	GGCC	2 cut(s)	818, 2139
HgaI	GACGC	4 cut(s)	890, 1160, 1231, 2384
HhaI	GCGC	1 cut(s)	1375
Hin6I	GCGC	1 cut(s)	1373
HinP1I	GCGC	1 cut(s)	1373
HincII	GTYRAC	4 cut(s)	64, 1185, 1243, 1434
HindII	GTYRAC	4 cut(s)	64, 1185, 1243, 1434
HindIII	AAGCTT	4 cut(s)	1007, 1367, 2326, 2363
Hpy99I	CGWCG	3 cut(s)	281, 884, 1225
HpyAV	CCTTC	8 cut(s)	262, 896, 1150, 1817, 1951, 2008, 2197, 2877
HpyCH4III	ACNGT	5 cut(s)	425, 824, 1893, 1949, 2585
HpyCH4IV	ACGT	2 cut(s)	276, 2228
HpySE526I	ACGT	2 cut(s)	276, 2228
HspAI	GCGC	1 cut(s)	1373
Kzo9I	GATC	8 cut(s)	34, 41, 610, 1738, 1939, 2128, 2335, 2692
LguI	GCTCTTC	2 cut(s)	668, 2624
LmnI	GCTCC	8 cut(s)	505, 736, 808, 1159, 2399, 2599, 2665, 2907
LweI	GCATC	5 cut(s)	517, 690, 1722, 1724, 2203
MaeI	CTAG	6 cut(s)	899, 2084, 2280, 2444, 2669, 2847
MaeII	ACGT	2 cut(s)	276, 2228
MalI	GATC	8 cut(s)	36, 43, 612, 1740, 1941, 2130, 2337, 2694
MboI	GATC	8 cut(s)	34, 41, 610, 1738, 1939, 2128, 2335, 2692
MfeI	CAATTG	1 cut(s)	1511
MflI	RGATCY	1 cut(s)	2692
MhlI	GDGCHC	6 cut(s)	620, 813, 1220, 1646, 2159, 2237
MlyI	GAGTC	7 cut(s)	14, 191, 332, 2282, 2549, 2554, 2771
MmeI	TCCRAC	7 cut(s)	159, 225, 315, 1056, 1080, 2377, 2745
MroXI	GAANNNNTTC	3 cut(s)	1982, 2147, 2264
MseI	TTAA	3 cut(s)	518, 645, 1922
MslI	CAYNNNNRTG	7 cut(s)	566, 1094, 2165, 2250, 2349, 2433, 2457
MspA1I	CMGCKG	2 cut(s)	805, 952
MspCI	CTTAAG	2 cut(s)	644, 1921
MspR9I	CCNGG	8 cut(s)	443, 734, 1117, 1231, 1820, 1821, 2503, 2677
MunI	CAATTG	1 cut(s)	1511
Mva1269I	GAATGC	1 cut(s)	18
MvaI	CCWGG	2 cut(s)	443, 734
MvnI	CGCG	1 cut(s)	1375
NciI	CCSGG	6 cut(s)	1117, 1231, 1820, 1821, 2503, 2677
NcoI	CCATGG	1 cut(s)	2428
NdeI	CATATG	1 cut(s)	2117
NdeII	GATC	8 cut(s)	34, 41, 610, 1738, 1939, 2128, 2335, 2692
NlaIV	GGNNCC	4 cut(s)	382, 1269, 1765, 2909
NmuCI	GTSAC	3 cut(s)	596, 2079, 2671
NspI	RCATGY	3 cut(s)	1138, 2249, 2350
NspV	TTCGAA	2 cut(s)	1319, 1427
OliI	CACNNNNGTG	1 cut(s)	2349
PaeR7I	CTCGAG	2 cut(s)	653, 869
PciI	ACATGT	2 cut(s)	1134, 2346
PciSI	GCTCTTC	2 cut(s)	668, 2624
PcsI	WCGNNNNNNNCGW	1 cut(s)	876
PctI	GAATGC	1 cut(s)	18
PdmI	GAANNNNTTC	3 cut(s)	1982, 2147, 2264
PflMI	CCANNNNNTGG	2 cut(s)	798, 2702
PinAI	ACCGGT	3 cut(s)	1043, 1187, 1760
PleI	GAGTC	7 cut(s)	13, 191, 332, 2282, 2549, 2554, 2771
PpsI	GAGTC	7 cut(s)	13, 191, 332, 2282, 2549, 2554, 2771
PscI	ACATGT	2 cut(s)	1134, 2346
Psp124BI	GAGCTC	2 cut(s)	813, 2237
Psp6I	CCWGG	2 cut(s)	441, 732
PspGI	CCWGG	2 cut(s)	441, 732
PspN4I	GGNNCC	4 cut(s)	382, 1269, 1765, 2909
PspPI	GGNCC	4 cut(s)	381, 816, 1046, 1334
PspXI	VCTCGAGB	1 cut(s)	653
PstI	CTGCAG	5 cut(s)	680, 951, 1832, 2226, 2273
PstNI	CAGNNNCTG	1 cut(s)	316
PsuI	RGATCY	1 cut(s)	2692
PvuII	CAGCTG	2 cut(s)	805, 952
RsaI	GTAC	9 cut(s)	427, 440, 981, 1028, 1519, 1770, 2312, 2658, 2820
RsaNI	GTAC	9 cut(s)	426, 439, 980, 1027, 1518, 1769, 2311, 2657, 2819
RseI	CAYNNNNRTG	7 cut(s)	566, 1094, 2165, 2250, 2349, 2433, 2457
SacI	GAGCTC	2 cut(s)	813, 2237
SapI	GCTCTTC	2 cut(s)	668, 2624
SaqAI	TTAA	3 cut(s)	518, 645, 1922
Sau3AI	GATC	8 cut(s)	34, 41, 610, 1738, 1939, 2128, 2335, 2692
Sau96I	GGNCC	4 cut(s)	381, 816, 1046, 1334
ScaI	AGTACT	3 cut(s)	1519, 2312, 2658
SchI	GAGTC	7 cut(s)	14, 191, 332, 2282, 2549, 2554, 2771
ScrFI	CCNGG	8 cut(s)	443, 734, 1117, 1231, 1820, 1821, 2503, 2677
SduI	GDGCHC	6 cut(s)	620, 813, 1220, 1646, 2159, 2237
SfaNI	GCATC	5 cut(s)	517, 690, 1722, 1724, 2203
SfcI	CTRYAG	6 cut(s)	676, 947, 1576, 1828, 2222, 2269
Sfr274I	CTCGAG	2 cut(s)	653, 869
SfuI	TTCGAA	2 cut(s)	1319, 1427
SinI	GGWCC	3 cut(s)	381, 1046, 1334
SlaI	CTCGAG	2 cut(s)	653, 869
SmaI	CCCGGG	1 cut(s)	1821
SmiMI	CAYNNNNRTG	7 cut(s)	566, 1094, 2165, 2250, 2349, 2433, 2457
SmlI	CTYRAG	6 cut(s)	415, 644, 653, 869, 1921, 2290
SmoI	CTYRAG	6 cut(s)	415, 644, 653, 869, 1921, 2290
SpeI	ACTAGT	1 cut(s)	2279
SsiI	CCGC	8 cut(s)	141, 144, 147, 234, 1127, 1375, 2136, 2510
SspMI	CTAG	6 cut(s)	899, 2084, 2280, 2444, 2669, 2847
SstI	GAGCTC	2 cut(s)	813, 2237
StyD4I	CCNGG	8 cut(s)	441, 732, 1115, 1229, 1818, 1819, 2501, 2675
StyI	CCWWGG	2 cut(s)	685, 2428
TaaI	ACNGT	5 cut(s)	425, 824, 1893, 1949, 2585
TaiI	ACGT	2 cut(s)	279, 2231
TatI	WGTACW	3 cut(s)	1517, 2310, 2656
TauI	GCSGC	4 cut(s)	143, 146, 149, 2139
Tru1I	TTAA	3 cut(s)	518, 645, 1922
Tru9I	TTAA	3 cut(s)	518, 645, 1922
TscAI	CASTG	5 cut(s)	1860, 1881, 2008, 2131, 2191
TseFI	GTSAC	3 cut(s)	596, 2079, 2671
Tsp45I	GTSAC	3 cut(s)	596, 2079, 2671
TspDTI	ATGAA	7 cut(s)	462, 503, 1078, 1169, 1941, 2375, 2441
TspGWI	ACGGA	2 cut(s)	321, 1744
TspMI	CCCGGG	1 cut(s)	1819
TspRI	CASTG	5 cut(s)	1860, 1881, 2008, 2131, 2191
Van91I	CCANNNNNTGG	2 cut(s)	798, 2702
Vha464I	CTTAAG	2 cut(s)	644, 1921
VneI	GTGCAC	1 cut(s)	1216
VpaK11BI	GGWCC	3 cut(s)	381, 1046, 1334
XapI	RAATTY	8 cut(s)	430, 765, 892, 989, 1105, 1194, 1660, 2207
XbaI	TCTAGA	1 cut(s)	898
XceI	RCATGY	3 cut(s)	1138, 2249, 2350
XhoI	CTCGAG	2 cut(s)	653, 869
XmaI	CCCGGG	1 cut(s)	1819
XmiI	GTMKAC	2 cut(s)	2070, 2304
XmnI	GAANNNNTTC	3 cut(s)	1982, 2147, 2264
XspI	CTAG	6 cut(s)	899, 2084, 2280, 2444, 2669, 2847
ZrmI	AGTACT	3 cut(s)	1519, 2312, 2658

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.