Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0004091

Physical Location & Seq

Genomic Coordinates Reverse (-)

35232997 .. 35235963

Gene Structure 2967 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000002493

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2880 bp

ATGCCATTACCCAAAAACCACCACCTCCATCTCTACCTCCTCTTCTTCCTCTTCCTCCTCTCCAACGCCGCCGCAGCCGACGAGCTTCAAATCCTGCTAAAACTCAAATCCTCCCTCCAAGACTCCAACGCCCAACTCTTCGCCACATGGGACTCCACCGCCAATTCGTTTTGCACGTTCACCGGAATCACCTGCAACAACGCTGGTTCGGTTCGAGAAATCGAACTTTCGAACCAGAAGCTGTCGGGGTCTCTTCCGTTGGACTCGATATGCCAACTTCCATCATTAGAAAAGCTCGCTTTCGGGTCCAATTTCCTGCACGGGACAATCACGGAGGACTTGAGAAACTGTACGAATTTGAAGTACCTGGATTTGGGGAAAAACTTGTTCACGGGTTCATTCCCTGACATATCTTCCTTGTCCCAACTGGAGCATCTTCATTTAAACGACAACGTGTTTTCGGGGATTTTCCCGTGGACTTGCCTCACCAACATGACTGGTCTGATTCGTTTGAGCTTGGGTAACAACCCGTTTGATCCGAGCCCGTTTCCAAAGGAAGTAGTAACTCTTAAGAAGCTCGAGTGGCTCCACTTAGCAAACTGCAGCATCCAAGGAACGATACCAAGCGAAATCGGGAACTTGGTCGAGCTTATCAACCTGGAGCTGTCCGGTAACAACATGACCGGAGAAATTCCTGCCGAGATTGGACAGCTCACTAAGCTCTGGCAGCTGGAGCTCTACACCAACAGGTTCACCGGGAAACTTCCTTTCGGGCTAAGAAACCTCACCAATCTCGAGAACTTCGACGCTTCCGCGAATTTTCTAGAAGGCGATTTGAACGAGTTGGGGTTTCTGACCAAGTTGGTTTCTCTGCAGCTGTCTAAGAACAATTTTTCAGGCGAAGTACCGGAAGAATTCGGCGAGTTCAAGAAGCTTGTGAATCTGTCTTTGTACGCTAACAAGCTGACCGGTCCTCTGCCTCAGAAATTGGGGTCTTGGTCGGAGATGGACTTCATCGATGTGTCGGAGAATTTCTTTACAGGGACTATTCCGCCGGACATGTGCAACAAAGGGACGATGAAGGAGCTGCTCATGCTTCAGAACAAGTTGACCGGTGAAATTCCGGCAAACTATGCGAAGTGCACGACGCTGACCCGGTTCAGGGTCAACAACAACTCCCTCTCCGGTGTGGTTCCGGCTGGACTTTGGGGATTGCCGAATGTGGCAATCATTGACATCACTTCGAATCAACTTGAAGGTCCGATTACTTCCGATATCGAAAACGCCAAGAAGCTTGCGCAGTTGTTTGTGAGTTACAATCGGTTATCTGGTGAATTACCGGACGAGCTTTCGAAGTCAACTTCGTTGGTTTCGGTTGTGTTGAATAACAATCAGTTTTCCGGTAAGATTCCGGCGAAGATAGGGGACTTGAAGCAATTGAGTACGCTGTTTTTACAGAGCAACTTGTTGTCTTCTTCAATTCCCAAGTCATTAGGAAGCTGTAGTTTCCTGAGTGACTTGAACATGGCAAACAACTCGCTTTCCGGTAAAATCCCGTCATCTTTGGGCTCTTTGCCGACCTTGAATTCTTTGGATTTGTCTCACAATCAACTCTCCGGTAAAATCCCGGAAAGTCTGGCATCTCTGAGACTAAGCATGCTTGATCTTTCGCACAACAGACTGACCGGTGCCATACCGGAATCTCTCTCAATTGCAGCCTACAACGGCGGCCTTTCTGGTAACGCGGGTCTCTGCAGCATGGACATCACCTACTTCCCACGGTGCTCGCCGGAAAAGGAAATGTCCAACGATGTCAGGACACTCATTATTTGCTTCTCAGTGGGTACACCAATATTTTTTGTTTCACTCATTTGCTTCTTGTTCTTAAAGAGGGAGGAGAAAGATCAAGACCGTTCATTGAAGCGAGAATCTTGGGATGTAAAGTCCTTCCATGTAATAACCTTCAGTGAGGATGAGATTCTTGATTCCATTACTCAAGAGAATCTAATTGCAAAAGGAGGTTCTGGAGATGTCTACAAAGTGTCACTAGCCAATGGCAAAGAACTCGCCGTGAAGCACATATGGAACACTGATCCAAGCGGCCGGAAAATGTTCAAGAGCACCACACCGATGCGAGGGAGACGGGGCAGTGGTAGTTCGAAATTGAAGGAATTTGATGCTGAGGTGCAGACGTTGAGCTCAATAAGGCATGTGAATGTGGTGAAGCTGTTCTGCAGCATTACTAGTGAGGACTCGAGCCTTTTGGTCTACGAGTACTTACCGAATGGAAGCTTGTGGGATCGCCTGCACATGTGTGAGAAAAGGAAGCTTGATTGGGACGCAAGGTATGAGATTGCAGTCGGAGCAGCCAAAGGGTTGGAGTATCTGCACCATGGCTGTGAGAGGCTAGTGATTCATAGAGATGTCAAGTCCAGTAACATTTTGTTAGACAAGTCTTTGAAGCCCCGGATTGCGGATTTTGGGCTTGCCAAGATGGTTCAGCCTAATGCAATCAAGGACTCGACTCATGTTGTTGCTGGAACACACGGTTACATAGCTCCTGAATATGGTTACACCTACAAGGTGAATGAGAAGAGCGATGTGTATAGCTTCGGTGTAGTACTAATGGAGCTAGTGACCGGGAAAAAACCGATAGATCCATCATTTGGGGACAACAAGGACATAGTGAACTGGATATGTGGCAACCTGAAGAGTAGAGAGAGTGTGTTAGGTGTGGTGGACTCATACATTCCTGAGGCATATAGAGAAGAGGCTATCAAGGTATTGAGAATTGCAATTTTGTGCACAGATAGGCTTCCAGAGCTAAGACCTTCCATGAAAAGTGTCGTTCAAATGCTTGAAGAAGCTCGGAGCCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001653 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009791 GO:0009845 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045087 GO:0046777 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0090351 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

960

Amino Acids

106.06

Weight (kDa)

6.05

Isoelectric Point (pI)

31.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	26 - 66	3.4e-09	Leucine rich repeat N-terminal domain
LRR_14	PF23598	185 - 297	6e-13	Leucine-rich repeat region
LRR_8	PF13855	504 - 561	1e-06	Leucine rich repeat
Pkinase	PF00069	666 - 951	5.7e-39	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	717 - 953	9.1e-37	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004091)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09970 AT1G09970
fragaria_vesca	FvH4_5g33590
malus_domestica	MD08G1210000.v1.1 MD15G1394100.v1.1
prunus_persica	Prupe.1G542300_v2.0.a1
pyrus_communis	pycom08g18120 pycom15g35400 pycom15g35410
rosa_chinensis	RchiOBHm_Chr7g0217331 RchiOBHm_Chr7g0232471
rosa_laevigata	RLG00000001335 RLG00000002489 RLG00000002493
rosa_multiflora	Rmu_sc0001898.1_g000015 Rmu_sc0004663.1_g000011 Rmu_sc0011824.1_g000016
rosa_roxburghii	Rroxscaffold_3G00228280 Rroxscaffold_3G00242430
rosa_rugosa	Rorug07G0168100 Rorug07G0274700
rosa_samantha	Rh7AG307400 Rh7AG307800 Rh7AG428100 Rh7BG298200 Rh7BG402600 Rh7CG326500 Rh7CG326900 Rh7CG447100 Rh7DG306900 Rh7DG418900
rosa_wichuraiana	Rw7G026090 Rw7G035470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	200
Acc16I	TGCGCA	1 cut(s)	1299
Acc36I	ACCTGC	1 cut(s)	200
AccB1I	GGYRCC	1 cut(s)	1688
AccB7I	CCANNNNNTGG	1 cut(s)	2666
AccI	GTMKAC	2 cut(s)	2034, 2268
AccII	CGCG	2 cut(s)	815, 1745
AciI	CCGC	9 cut(s)	69, 72, 159, 813, 1052, 1728, 1745, 2100, 2474
AclWI	GGATC	4 cut(s)	530, 2087, 2307, 2651
AcoI	YGGCCR	1 cut(s)	2101
AcsI	RAATTY	8 cut(s)	355, 690, 817, 914, 1030, 1119, 1585, 2171
AcuI	CTGAAG	3 cut(s)	1082, 1948, 2729
AfaI	GTAC	8 cut(s)	352, 365, 906, 953, 1444, 1846, 2276, 2622
AfiI	CCNNNNNNNGG	7 cut(s)	373, 1161, 1162, 2135, 2473, 2567, 2666
AflII	CTTAAG	1 cut(s)	569
AflIII	ACRYGT	3 cut(s)	453, 1059, 2310
AgeI	ACCGGT	3 cut(s)	968, 1112, 1685
AhlI	ACTAGT	1 cut(s)	2243
AjnI	CCWGG	2 cut(s)	366, 657
AleI	CACNNNNGTG	1 cut(s)	2313
Alw21I	GWGCWC	6 cut(s)	738, 1145, 1787, 2123, 2201, 2807
Alw26I	GTCTC	5 cut(s)	255, 1605, 1642, 1754, 2134
Alw44I	GTGCAC	2 cut(s)	1141, 2803
AlwI	GGATC	4 cut(s)	530, 2087, 2307, 2651
AlwNI	CAGNNNCTG	1 cut(s)	241
Ama87I	CYCGRG	3 cut(s)	578, 794, 2254
AoxI	GGCC	2 cut(s)	1729, 2101
ApaLI	GTGCAC	2 cut(s)	1141, 2803
ApeKI	GCWGC	9 cut(s)	74, 603, 727, 874, 1087, 1715, 1755, 2235, 2366
ApoI	RAATTY	8 cut(s)	355, 690, 817, 914, 1030, 1119, 1585, 2171
AsiGI	ACCGGT	3 cut(s)	968, 1112, 1685
Asp700I	GAANNNNTTC	2 cut(s)	2111, 2228
AspLEI	GCGC	1 cut(s)	1300
AspS9I	GGNCC	3 cut(s)	306, 971, 1259
AsuC2I	CCSGG	5 cut(s)	757, 1156, 1628, 2467, 2641
AsuII	TTCGAA	4 cut(s)	230, 1244, 1352, 2159
AvaI	CYCGRG	3 cut(s)	578, 794, 2254
AvaII	GGWCC	3 cut(s)	306, 971, 1259
AxyI	CCTNAGG	1 cut(s)	2754
BaeGI	GKGCMC	2 cut(s)	1145, 2807
BanI	GGYRCC	1 cut(s)	1688
BanII	GRGCYC	4 cut(s)	545, 738, 1571, 2201
BbsI	GAAGAC	1 cut(s)	1464
Bbv12I	GWGCWC	6 cut(s)	738, 1145, 1787, 2123, 2201, 2807
BbvCI	CCTCAGC	1 cut(s)	2181
BbvI	GCAGC	9 cut(s)	86, 615, 739, 886, 1074, 1727, 1767, 2247, 2378
BccI	CCATC	5 cut(s)	36, 289, 1000, 2488, 2668
BceAI	ACGGC	2 cut(s)	1741, 2054
BcgI	CGANNNNNNTGC	4 cut(s)	1518, 1552, 2047, 2081
BciT130I	CCWGG	2 cut(s)	368, 659
BcnI	CCSGG	5 cut(s)	757, 1156, 1628, 2467, 2641
BcoDI	GTCTC	5 cut(s)	255, 1605, 1642, 1754, 2134
BcuI	ACTAGT	1 cut(s)	2243
BfaI	CTAG	5 cut(s)	824, 2048, 2244, 2408, 2633
BfmI	CTRYAG	5 cut(s)	601, 872, 1501, 1753, 2233
BfrI	CTTAAG	1 cut(s)	569
BfuAI	ACCTGC	1 cut(s)	200
BmcAI	AGTACT	2 cut(s)	2276, 2622
Bme1390I	CCNGG	7 cut(s)	368, 659, 757, 1156, 1628, 2467, 2641
Bme18I	GGWCC	3 cut(s)	306, 971, 1259
BmeT110I	CYCGRG	3 cut(s)	578, 794, 2254
BmgT120I	GGNCC	3 cut(s)	306, 971, 1259
BmiI	GGNNCC	5 cut(s)	307, 587, 1194, 1690, 2873
BmrFI	CCNGG	7 cut(s)	368, 659, 757, 1156, 1628, 2467, 2641
BmsI	GCATC	5 cut(s)	442, 615, 1649, 2121, 2167
BpiI	GAAGAC	1 cut(s)	1464
BpmI	CTGGAG	4 cut(s)	449, 680, 752, 2046
Bpu10I	CCTNAGC	1 cut(s)	2181
Bpu14I	TTCGAA	4 cut(s)	230, 1244, 1352, 2159
BpuEI	CTTGAG	2 cut(s)	361, 1980
BpuMI	CCSGG	5 cut(s)	757, 1156, 1628, 2467, 2641
Bsa29I	ATCGAT	1 cut(s)	1017
BsaI	GGTCTC	2 cut(s)	255, 1754
BsaJI	CCNNGG	5 cut(s)	473, 610, 1778, 2392, 2465
BsaXI	ACNNNNNCTCC	4 cut(s)	1160, 1166, 1190, 1196
Bsc4I	CCNNNNNNNGG	7 cut(s)	373, 1161, 1162, 2135, 2473, 2567, 2666
Bse118I	RCCGGY	3 cut(s)	968, 1112, 1685
Bse1I	ACTGG	4 cut(s)	432, 502, 2433, 2696
Bse21I	CCTNAGG	1 cut(s)	2754
BseBI	CCWGG	2 cut(s)	368, 659
BseCI	ATCGAT	1 cut(s)	1017
BseDI	CCNNGG	5 cut(s)	473, 610, 1778, 2392, 2465
BseGI	GGATG	3 cut(s)	606, 1942, 1978
BseLI	CCNNNNNNNGG	7 cut(s)	373, 1161, 1162, 2135, 2473, 2567, 2666
BseMII	CTCAG	6 cut(s)	995, 1502, 1637, 1851, 2172, 2745
BseNI	ACTGG	4 cut(s)	432, 502, 2433, 2696
BseRI	GAGGAG	3 cut(s)	29, 47, 1910
BseSI	GKGCMC	2 cut(s)	1145, 2807
BseX3I	CGGCCG	1 cut(s)	2101
BseXI	GCAGC	9 cut(s)	86, 615, 739, 886, 1074, 1727, 1767, 2247, 2378
BsgI	GTGCAG	4 cut(s)	302, 2207, 2291, 2372
Bsh1236I	CGCG	2 cut(s)	815, 1745
Bsh1285I	CGRYCG	1 cut(s)	2104
BshFI	GGCC	2 cut(s)	1731, 2103
BshNI	GGYRCC	1 cut(s)	1688
BshTI	ACCGGT	3 cut(s)	968, 1112, 1685
BshVI	ATCGAT	1 cut(s)	1017
BsiEI	CGRYCG	1 cut(s)	2104
BsiHKAI	GWGCWC	6 cut(s)	738, 1145, 1787, 2123, 2201, 2807
BsiHKCI	CYCGRG	3 cut(s)	578, 794, 2254
BslFI	GGGAC	8 cut(s)	164, 337, 406, 1057, 1087, 1439, 2351, 2684
BslI	CCNNNNNNNGG	7 cut(s)	373, 1161, 1162, 2135, 2473, 2567, 2666
BsmAI	GTCTC	5 cut(s)	255, 1605, 1642, 1754, 2134
BsmBI	CGTCTC	1 cut(s)	2134
BsmFI	GGGAC	8 cut(s)	164, 337, 406, 1057, 1087, 1439, 2351, 2684
BsnI	GGCC	2 cut(s)	1731, 2103
Bso31I	GGTCTC	2 cut(s)	255, 1754
BsoBI	CYCGRG	3 cut(s)	578, 794, 2254
Bsp119I	TTCGAA	4 cut(s)	230, 1244, 1352, 2159
Bsp1286I	GDGCHC	8 cut(s)	545, 738, 1145, 1571, 1787, 2123, 2201, 2807
Bsp143I	GATC	6 cut(s)	535, 1663, 1903, 2092, 2299, 2656
Bsp19I	CCATGG	1 cut(s)	2392
BspACI	CCGC	9 cut(s)	69, 72, 159, 813, 1052, 1728, 1745, 2100, 2474
BspANI	GGCC	2 cut(s)	1731, 2103
BspCNI	CTCAG	6 cut(s)	994, 1503, 1638, 1850, 2173, 2746
BspDI	ATCGAT	1 cut(s)	1017
BspFNI	CGCG	2 cut(s)	815, 1745
BspLI	GGNNCC	5 cut(s)	307, 587, 1194, 1690, 2873
BspMAI	CTGCAG	4 cut(s)	605, 876, 1757, 2237
BspMI	ACCTGC	1 cut(s)	200
BspPI	GGATC	4 cut(s)	530, 2087, 2307, 2651
BspQI	GCTCTTC	1 cut(s)	2588
BspT104I	TTCGAA	4 cut(s)	230, 1244, 1352, 2159
BspT107I	GGYRCC	1 cut(s)	1688
BspTI	CTTAAG	1 cut(s)	569
BspTNI	GGTCTC	2 cut(s)	255, 1754
BsrFI	RCCGGY	3 cut(s)	968, 1112, 1685
BsrI	ACTGG	4 cut(s)	432, 502, 2433, 2696
BssAI	RCCGGY	3 cut(s)	968, 1112, 1685
BssECI	CCNNGG	5 cut(s)	473, 610, 1778, 2392, 2465
BssMI	GATC	6 cut(s)	535, 1663, 1903, 2092, 2299, 2656
BssT1I	CCWWGG	2 cut(s)	610, 2392
Bst2UI	CCWGG	2 cut(s)	368, 659
Bst4CI	ACNGT	4 cut(s)	350, 1782, 1913, 2549
Bst6I	CTCTTC	7 cut(s)	47, 56, 143, 258, 2588, 2705, 2763
BstAFI	CTTAAG	1 cut(s)	569
BstAPI	GCANNNNNTGC	1 cut(s)	1133
BstBI	TTCGAA	4 cut(s)	230, 1244, 1352, 2159
BstC8I	GCNNGC	6 cut(s)	297, 1296, 1658, 1787, 2306, 2487
BstDSI	CCRYGG	3 cut(s)	473, 1778, 2392
BstF5I	GGATG	3 cut(s)	606, 1942, 1978
BstFNI	CGCG	2 cut(s)	815, 1745
BstHHI	GCGC	1 cut(s)	1300
BstKTI	GATC	6 cut(s)	538, 1666, 1906, 2095, 2302, 2659
BstMAI	GTCTC	5 cut(s)	255, 1605, 1642, 1754, 2134
BstMBI	GATC	6 cut(s)	535, 1663, 1903, 2092, 2299, 2656
BstMCI	CGRYCG	1 cut(s)	2104
BstMWI	GCNNNNNNNGC	9 cut(s)	74, 583, 718, 727, 733, 1093, 1133, 2363, 2821
BstNI	CCWGG	2 cut(s)	368, 659
BstNSI	RCATGY	4 cut(s)	1063, 1660, 2213, 2314
BstSCI	CCNGG	7 cut(s)	366, 657, 755, 1154, 1626, 2465, 2639
BstSFI	CTRYAG	5 cut(s)	601, 872, 1501, 1753, 2233
BstSLI	GKGCMC	2 cut(s)	1145, 2807
BstUI	CGCG	2 cut(s)	815, 1745
BstV1I	GCAGC	9 cut(s)	86, 615, 739, 886, 1074, 1727, 1767, 2247, 2378
BstV2I	GAAGAC	1 cut(s)	1464
BstX2I	RGATCY	1 cut(s)	2656
BstXI	CCANNNNNNTGG	1 cut(s)	2377
BstYI	RGATCY	1 cut(s)	2656
BstZI	CGGCCG	1 cut(s)	2101
Bsu15I	ATCGAT	1 cut(s)	1017
Bsu36I	CCTNAGG	1 cut(s)	2754
BsuRI	GGCC	2 cut(s)	1731, 2103
BsuTUI	ATCGAT	1 cut(s)	1017
BtgI	CCRYGG	3 cut(s)	473, 1778, 2392
BtgZI	GCGATG	1 cut(s)	2613
BtsCI	GGATG	3 cut(s)	606, 1942, 1978
BtsI	GCAGTG	1 cut(s)	2155
BtsIMutI	CAGTG	4 cut(s)	1845, 1972, 2088, 2155
BveI	ACCTGC	1 cut(s)	200
Cac8I	GCNNGC	6 cut(s)	297, 1296, 1658, 1787, 2306, 2487
CaiI	CAGNNNCTG	1 cut(s)	241
CfoI	GCGC	1 cut(s)	1300
Cfr10I	RCCGGY	3 cut(s)	968, 1112, 1685
Cfr13I	GGNCC	3 cut(s)	306, 971, 1259
ClaI	ATCGAT	1 cut(s)	1017
CseI	GACGC	3 cut(s)	815, 1156, 2348
Csp6I	GTAC	8 cut(s)	351, 364, 905, 952, 1443, 1845, 2275, 2621
CspAI	ACCGGT	3 cut(s)	968, 1112, 1685
CviQI	GTAC	8 cut(s)	351, 364, 905, 952, 1443, 1845, 2275, 2621
DpnI	GATC	6 cut(s)	537, 1665, 1905, 2094, 2301, 2658
DpnII	GATC	6 cut(s)	535, 1663, 1903, 2092, 2299, 2656
DraI	TTTAAA	1 cut(s)	444
EaeI	YGGCCR	1 cut(s)	2101
EagI	CGGCCG	1 cut(s)	2101
Eam1104I	CTCTTC	7 cut(s)	47, 56, 143, 258, 2588, 2705, 2763
EarI	CTCTTC	7 cut(s)	47, 56, 143, 258, 2588, 2705, 2763
EciI	GGCGGA	1 cut(s)	1041
Ecl136II	GAGCTC	2 cut(s)	736, 2199
EclXI	CGGCCG	1 cut(s)	2101
Eco130I	CCWWGG	2 cut(s)	610, 2392
Eco24I	GRGCYC	4 cut(s)	545, 738, 1571, 2201
Eco31I	GGTCTC	2 cut(s)	255, 1754
Eco32I	GATATC	1 cut(s)	1276
Eco47I	GGWCC	3 cut(s)	306, 971, 1259
Eco52I	CGGCCG	1 cut(s)	2101
Eco53kI	GAGCTC	2 cut(s)	736, 2199
Eco57I	CTGAAG	3 cut(s)	1082, 1948, 2729
Eco81I	CCTNAGG	1 cut(s)	2754
Eco88I	CYCGRG	3 cut(s)	578, 794, 2254
EcoICRI	GAGCTC	2 cut(s)	736, 2199
EcoRI	GAATTC	2 cut(s)	914, 1585
EcoRII	CCWGG	2 cut(s)	366, 657
EcoRV	GATATC	1 cut(s)	1276
EcoT14I	CCWWGG	2 cut(s)	610, 2392
EcoT38I	GRGCYC	4 cut(s)	545, 738, 1571, 2201
ErhI	CCWWGG	2 cut(s)	610, 2392
Esp3I	CGTCTC	1 cut(s)	2134
FaqI	GGGAC	8 cut(s)	164, 337, 406, 1057, 1087, 1439, 2351, 2684
FauI	CCCGC	1 cut(s)	1738
FauNDI	CATATG	1 cut(s)	2081
FblI	GTMKAC	2 cut(s)	2034, 2268
FokI	GGATG	3 cut(s)	593, 1949, 1985
FriOI	GRGCYC	4 cut(s)	545, 738, 1571, 2201
FspBI	CTAG	5 cut(s)	824, 2048, 2244, 2408, 2633
FspI	TGCGCA	1 cut(s)	1299
GlaI	GCGC	1 cut(s)	1299
GsuI	CTGGAG	4 cut(s)	449, 680, 752, 2046
HaeIII	GGCC	2 cut(s)	1731, 2103
HgaI	GACGC	3 cut(s)	815, 1156, 2348
HhaI	GCGC	1 cut(s)	1300
Hin6I	GCGC	1 cut(s)	1298
HinP1I	GCGC	1 cut(s)	1298
HincII	GTYRAC	3 cut(s)	1110, 1168, 1359
HindII	GTYRAC	3 cut(s)	1110, 1168, 1359
HindIII	AAGCTT	4 cut(s)	932, 1292, 2290, 2327
Hpy99I	CGWCG	3 cut(s)	83, 809, 1150
HpyAV	CCTTC	7 cut(s)	821, 1075, 1250, 1957, 1972, 2161, 2841
HpyCH4III	ACNGT	4 cut(s)	350, 1782, 1913, 2549
HpyCH4IV	ACGT	3 cut(s)	176, 453, 2192
HpyF10VI	GCNNNNNNNGC	9 cut(s)	74, 583, 718, 727, 733, 1093, 1133, 2363, 2821
HpySE526I	ACGT	3 cut(s)	176, 453, 2192
HspAI	GCGC	1 cut(s)	1298
Kzo9I	GATC	6 cut(s)	535, 1663, 1903, 2092, 2299, 2656
LguI	GCTCTTC	1 cut(s)	2588
LmnI	GCTCC	9 cut(s)	430, 591, 661, 733, 1084, 2363, 2563, 2629, 2871
Lsp1109I	GCAGC	9 cut(s)	86, 615, 739, 886, 1074, 1727, 1767, 2247, 2378
LweI	GCATC	5 cut(s)	442, 615, 1649, 2121, 2167
MaeI	CTAG	5 cut(s)	824, 2048, 2244, 2408, 2633
MaeII	ACGT	3 cut(s)	176, 453, 2192
MalI	GATC	6 cut(s)	537, 1665, 1905, 2094, 2301, 2658
MboI	GATC	6 cut(s)	535, 1663, 1903, 2092, 2299, 2656
MfeI	CAATTG	2 cut(s)	1436, 1710
MflI	RGATCY	1 cut(s)	2656
MhlI	GDGCHC	8 cut(s)	545, 738, 1145, 1571, 1787, 2123, 2201, 2807
MlyI	GAGTC	7 cut(s)	116, 146, 257, 2246, 2513, 2518, 2735
MmeI	TCCRAC	8 cut(s)	87, 150, 240, 981, 1005, 1830, 2341, 2358
MroXI	GAANNNNTTC	2 cut(s)	2111, 2228
MseI	TTAA	3 cut(s)	443, 570, 1886
MslI	CAYNNNNRTG	7 cut(s)	491, 1019, 2129, 2214, 2313, 2397, 2421
MspA1I	CMGCKG	2 cut(s)	730, 877
MspCI	CTTAAG	1 cut(s)	569
MspR9I	CCNGG	7 cut(s)	368, 659, 757, 1156, 1628, 2467, 2641
MunI	CAATTG	2 cut(s)	1436, 1710
MvaI	CCWGG	2 cut(s)	368, 659
MvnI	CGCG	2 cut(s)	815, 1745
MwoI	GCNNNNNNNGC	9 cut(s)	74, 583, 718, 727, 733, 1093, 1133, 2363, 2821
NciI	CCSGG	5 cut(s)	757, 1156, 1628, 2467, 2641
NcoI	CCATGG	1 cut(s)	2392
NdeI	CATATG	1 cut(s)	2081
NdeII	GATC	6 cut(s)	535, 1663, 1903, 2092, 2299, 2656
NlaIV	GGNNCC	5 cut(s)	307, 587, 1194, 1690, 2873
NmeAIII	GCCGAG	1 cut(s)	724
NmuCI	GTSAC	3 cut(s)	1514, 2043, 2635
NsbI	TGCGCA	1 cut(s)	1299
NspI	RCATGY	4 cut(s)	1063, 1660, 2213, 2314
NspV	TTCGAA	4 cut(s)	230, 1244, 1352, 2159
OliI	CACNNNNGTG	1 cut(s)	2313
PaeI	GCATGC	1 cut(s)	1660
PaeR7I	CTCGAG	3 cut(s)	578, 794, 2254
PaqCI	CACCTGC	1 cut(s)	200
PciI	ACATGT	2 cut(s)	1059, 2310
PciSI	GCTCTTC	1 cut(s)	2588
PcsI	WCGNNNNNNNCGW	2 cut(s)	173, 801
PdmI	GAANNNNTTC	2 cut(s)	2111, 2228
PflMI	CCANNNNNTGG	1 cut(s)	2666
PfoI	TCCNGGA	1 cut(s)	1626
PinAI	ACCGGT	3 cut(s)	968, 1112, 1685
PleI	GAGTC	7 cut(s)	116, 146, 257, 2246, 2513, 2518, 2735
PpsI	GAGTC	7 cut(s)	116, 146, 257, 2246, 2513, 2518, 2735
PscI	ACATGT	2 cut(s)	1059, 2310
Psp124BI	GAGCTC	2 cut(s)	738, 2201
Psp6I	CCWGG	2 cut(s)	366, 657
PspGI	CCWGG	2 cut(s)	366, 657
PspN4I	GGNNCC	5 cut(s)	307, 587, 1194, 1690, 2873
PspPI	GGNCC	3 cut(s)	306, 971, 1259
PspXI	VCTCGAGB	2 cut(s)	578, 2254
PstI	CTGCAG	4 cut(s)	605, 876, 1757, 2237
PstNI	CAGNNNCTG	1 cut(s)	241
PsuI	RGATCY	1 cut(s)	2656
PvuII	CAGCTG	2 cut(s)	730, 877
RsaI	GTAC	8 cut(s)	352, 365, 906, 953, 1444, 1846, 2276, 2622
RsaNI	GTAC	8 cut(s)	351, 364, 905, 952, 1443, 1845, 2275, 2621
RseI	CAYNNNNRTG	7 cut(s)	491, 1019, 2129, 2214, 2313, 2397, 2421
SacI	GAGCTC	2 cut(s)	738, 2201
SapI	GCTCTTC	1 cut(s)	2588
SaqAI	TTAA	3 cut(s)	443, 570, 1886
Sau3AI	GATC	6 cut(s)	535, 1663, 1903, 2092, 2299, 2656
Sau96I	GGNCC	3 cut(s)	306, 971, 1259
ScaI	AGTACT	2 cut(s)	2276, 2622
SchI	GAGTC	7 cut(s)	116, 146, 257, 2246, 2513, 2518, 2735
ScrFI	CCNGG	7 cut(s)	368, 659, 757, 1156, 1628, 2467, 2641
SduI	GDGCHC	8 cut(s)	545, 738, 1145, 1571, 1787, 2123, 2201, 2807
SfaNI	GCATC	5 cut(s)	442, 615, 1649, 2121, 2167
SfcI	CTRYAG	5 cut(s)	601, 872, 1501, 1753, 2233
Sfr274I	CTCGAG	3 cut(s)	578, 794, 2254
SfuI	TTCGAA	4 cut(s)	230, 1244, 1352, 2159
SinI	GGWCC	3 cut(s)	306, 971, 1259
SlaI	CTCGAG	3 cut(s)	578, 794, 2254
SmiMI	CAYNNNNRTG	7 cut(s)	491, 1019, 2129, 2214, 2313, 2397, 2421
SmlI	CTYRAG	6 cut(s)	340, 569, 578, 794, 1995, 2254
SmoI	CTYRAG	6 cut(s)	340, 569, 578, 794, 1995, 2254
SpeI	ACTAGT	1 cut(s)	2243
SphI	GCATGC	1 cut(s)	1660
SsiI	CCGC	9 cut(s)	69, 72, 159, 813, 1052, 1728, 1745, 2100, 2474
SspI	AATATT	1 cut(s)	1854
SspMI	CTAG	5 cut(s)	824, 2048, 2244, 2408, 2633
SstI	GAGCTC	2 cut(s)	738, 2201
StyD4I	CCNGG	7 cut(s)	366, 657, 755, 1154, 1626, 2465, 2639
StyI	CCWWGG	2 cut(s)	610, 2392
TaaI	ACNGT	4 cut(s)	350, 1782, 1913, 2549
TaiI	ACGT	3 cut(s)	179, 456, 2195
TatI	WGTACW	2 cut(s)	2274, 2620
TauI	GCSGC	4 cut(s)	71, 74, 1731, 2103
Tru1I	TTAA	3 cut(s)	443, 570, 1886
Tru9I	TTAA	3 cut(s)	443, 570, 1886
TscAI	CASTG	4 cut(s)	1845, 1972, 2095, 2155
TseFI	GTSAC	3 cut(s)	1514, 2043, 2635
TseI	GCWGC	9 cut(s)	74, 603, 727, 874, 1087, 1715, 1755, 2235, 2366
Tsp45I	GTSAC	3 cut(s)	1514, 2043, 2635
TspDTI	ATGAA	7 cut(s)	387, 428, 1003, 1094, 1905, 2405, 2852
TspGWI	ACGGA	2 cut(s)	246, 347
TspRI	CASTG	4 cut(s)	1845, 1972, 2095, 2155
Van91I	CCANNNNNTGG	1 cut(s)	2666
Vha464I	CTTAAG	1 cut(s)	569
VneI	GTGCAC	2 cut(s)	1141, 2803
VpaK11BI	GGWCC	3 cut(s)	306, 971, 1259
XapI	RAATTY	8 cut(s)	355, 690, 817, 914, 1030, 1119, 1585, 2171
XbaI	TCTAGA	1 cut(s)	823
XceI	RCATGY	4 cut(s)	1063, 1660, 2213, 2314
XhoI	CTCGAG	3 cut(s)	578, 794, 2254
XmiI	GTMKAC	2 cut(s)	2034, 2268
XmnI	GAANNNNTTC	2 cut(s)	2111, 2228
XspI	CTAG	5 cut(s)	824, 2048, 2244, 2408, 2633
ZrmI	AGTACT	2 cut(s)	2276, 2622

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.