MD08G1216500.v1.1
MADS Family

Agamous-like MADS-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0003722
Physical Location & Seq
Forward (+)
27928774 .. 27937925
9152 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1216500.v1.1.491

Sequence Viewer

Length: 672 bp
ATGGGGAGGGGAAAGATTGAAATCAAGAGGATCGAGAACACGACGAATCGGCAAGTGACCTTCTGCAAACGAAGAAATGGGCTGCTGAAGAAAGCATATGAACTATCCATTCTCTGTGATGCTGAAGTTGCCCTCATTGTCTTCTCTACTCGTGGCCGTCTCTACGAGTACTCTAACAACAACATAAGAAACACCATAGAGAGGTACAAGAAGGCATGTTCAGATAGCACAGGATCATCCTCTGTTACTGAAATTAACGCTCAGTATTATCAACAGGAATCGGCAAAACTGAGGCAACAGATTCAAATGCTGCAGAACTCTAACAGGCACTTAATGGGAGATGCCTTGAGCACTCTGACTGTGAAAGAACTAAAGCAGGTGGAGAATAGGCTTGAACGAGGCATTACTAGAATCAGGTCCAAGAAGCATGAACTGCTGCTTGCAGAGATTGAGTACTTCCAGAAAAAGGAGATTGAGCTGGAAAACGAAAATGTTTATCTTCGAACTAAGGTATCAGAAGTCGAGAGGCTTCAACAAGCAAACATGGTTTCTGGGTCAGAGATGAATGCAATCCAGGCATTAGCTTCTCGCCATTTCTTTAGCCAGAATATGATCGAGGGTGGAGAAGCTACCTTCCCACAGCAAGACAAGAAGAACCTCCATCTTGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
K-box SRF-TF

Protein Analysis

224

Amino Acids

25.78

Weight (kDa)

9.38

Isoelectric Point (pI)

54.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 10 - 57 1.5e-26 SRF-type transcription factor (DNA-binding and dimerisation domain)
K-box PF01486 86 - 171 4e-28 K-box region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 367
Acc36I ACCTGC 1 cut(s) 367
AclWI GGATC 2 cut(s) 38, 241
AcoI YGGCCR 1 cut(s) 154
AcuI CTGAAG 2 cut(s) 107, 144
AfaI GTAC 3 cut(s) 170, 206, 455
AfiI CCNNNNNNNGG 2 cut(s) 201, 466
AgsI TTSAA 4 cut(s) 20, 305, 395, 533
AjnI CCWGG 1 cut(s) 573
AluBI AGCT 3 cut(s) 478, 584, 629
AluI AGCT 3 cut(s) 478, 584, 629
Alw21I GWGCWC 1 cut(s) 353
Alw26I GTCTC 1 cut(s) 164
AlwI GGATC 2 cut(s) 38, 241
AoxI GGCC 1 cut(s) 154
ApeKI GCWGC 3 cut(s) 82, 310, 436
AspS9I GGNCC 1 cut(s) 417
AsuII TTCGAA 1 cut(s) 502
AvaII GGWCC 1 cut(s) 417
BaeI ACNNNNGTAYC 2 cut(s) 495, 528
BauI CACGAG 1 cut(s) 150
BbsI GAAGAC 1 cut(s) 133
Bbv12I GWGCWC 1 cut(s) 353
BbvI GCAGC 3 cut(s) 69, 297, 423
BccI CCATC 1 cut(s) 669
BceAI ACGGC 1 cut(s) 141
BciT130I CCWGG 1 cut(s) 575
BcoDI GTCTC 1 cut(s) 164
BfaI CTAG 1 cut(s) 408
BfmI CTRYAG 1 cut(s) 311
BfuAI ACCTGC 1 cut(s) 367
BisI GCNGC 3 cut(s) 83, 311, 437
BlsI GCNGC 3 cut(s) 84, 312, 438
BmcAI AGTACT 2 cut(s) 170, 455
Bme1390I CCNGG 1 cut(s) 575
Bme18I GGWCC 1 cut(s) 417
BmgT120I GGNCC 1 cut(s) 417
BmrFI CCNGG 1 cut(s) 575
BmsI GCATC 2 cut(s) 109, 331
BpiI GAAGAC 1 cut(s) 133
Bpu14I TTCGAA 1 cut(s) 502
BpuEI CTTGAG 1 cut(s) 367
BsaBI GATNNNNATC 1 cut(s) 20
Bsc4I CCNNNNNNNGG 2 cut(s) 201, 466
Bse8I GATNNNNATC 1 cut(s) 20
BseBI CCWGG 1 cut(s) 575
BseGI GGATG 1 cut(s) 236
BseJI GATNNNNATC 1 cut(s) 20
BseLI CCNNNNNNNGG 2 cut(s) 201, 466
BseMII CTCAG 2 cut(s) 275, 281
BseXI GCAGC 3 cut(s) 69, 297, 423
BshFI GGCC 1 cut(s) 156
BsiHKAI GWGCWC 1 cut(s) 353
BslI CCNNNNNNNGG 2 cut(s) 201, 466
BsmAI GTCTC 1 cut(s) 164
BsmBI CGTCTC 1 cut(s) 164
BsmI GAATGC 1 cut(s) 571
BsnI GGCC 1 cut(s) 156
Bsp119I TTCGAA 1 cut(s) 502
Bsp1286I GDGCHC 1 cut(s) 353
Bsp143I GATC 3 cut(s) 30, 233, 612
BspANI GGCC 1 cut(s) 156
BspCNI CTCAG 2 cut(s) 274, 282
BspMAI CTGCAG 1 cut(s) 315
BspMI ACCTGC 1 cut(s) 367
BspPI GGATC 2 cut(s) 38, 241
BspT104I TTCGAA 1 cut(s) 502
BssMI GATC 3 cut(s) 30, 233, 612
BssSI CACGAG 1 cut(s) 150
Bst2BI CACGAG 1 cut(s) 150
Bst2UI CCWGG 1 cut(s) 575
Bst4CI ACNGT 1 cut(s) 361
BstAPI GCANNNNNTGC 1 cut(s) 433
BstBI TTCGAA 1 cut(s) 502
BstC8I GCNNGC 1 cut(s) 441
BstDEI CTNAG 3 cut(s) 261, 290, 507
BstF5I GGATG 1 cut(s) 236
BstKTI GATC 3 cut(s) 33, 236, 615
BstMAI GTCTC 1 cut(s) 164
BstMBI GATC 3 cut(s) 30, 233, 612
BstMWI GCNNNNNNNGC 3 cut(s) 128, 433, 575
BstNI CCWGG 1 cut(s) 575
BstNSI RCATGY 1 cut(s) 219
BstSCI CCNGG 1 cut(s) 573
BstSFI CTRYAG 1 cut(s) 311
BstV1I GCAGC 3 cut(s) 69, 297, 423
BstV2I GAAGAC 1 cut(s) 133
BsuRI GGCC 1 cut(s) 156
BtsCI GGATG 1 cut(s) 236
BveI ACCTGC 1 cut(s) 367
Cac8I GCNNGC 1 cut(s) 441
Cfr13I GGNCC 1 cut(s) 417
Csp6I GTAC 3 cut(s) 169, 205, 454
CviAII CATG 3 cut(s) 216, 428, 544
CviJI RGCY 8 cut(s) 82, 156, 391, 478, 529, 584, 603, 629
CviKI_1 RGCY 8 cut(s) 82, 156, 391, 478, 529, 584, 603, 629
CviQI GTAC 3 cut(s) 169, 205, 454
DdeI CTNAG 3 cut(s) 261, 290, 507
DpnI GATC 3 cut(s) 32, 235, 614
DpnII GATC 3 cut(s) 30, 233, 612
EaeI YGGCCR 1 cut(s) 154
Eco47I GGWCC 1 cut(s) 417
Eco57I CTGAAG 2 cut(s) 107, 144
EcoRII CCWGG 1 cut(s) 573
Esp3I CGTCTC 1 cut(s) 164
FaeI CATG 3 cut(s) 219, 431, 547
FaiI YATR 8 cut(s) 97, 99, 185, 197, 217, 429, 545, 611
FatI CATG 3 cut(s) 215, 427, 543
FauNDI CATATG 1 cut(s) 97
Fnu4HI GCNGC 3 cut(s) 83, 311, 437
FokI GGATG 1 cut(s) 223
Fsp4HI GCNGC 3 cut(s) 83, 311, 437
FspBI CTAG 1 cut(s) 408
GluI GCNGC 3 cut(s) 83, 311, 437
HaeIII GGCC 1 cut(s) 156
Hin1II CATG 3 cut(s) 219, 431, 547
HinfI GANTC 4 cut(s) 46, 278, 301, 411
Hpy188I TCNGA 4 cut(s) 223, 357, 517, 559
Hpy188III TCNNGA 4 cut(s) 25, 34, 460, 523
Hpy99I CGWCG 1 cut(s) 46
HpyAV CCTTC 3 cut(s) 70, 205, 643
HpyCH4III ACNGT 1 cut(s) 361
HpyCH4V TGCA 4 cut(s) 66, 313, 443, 569
HpyF10VI GCNNNNNNNGC 3 cut(s) 128, 433, 575
HpyF3I CTNAG 3 cut(s) 261, 290, 507
Hsp92II CATG 3 cut(s) 219, 431, 547
Kzo9I GATC 3 cut(s) 30, 233, 612
Lsp1109I GCAGC 3 cut(s) 69, 297, 423
LweI GCATC 2 cut(s) 109, 331
MaeI CTAG 1 cut(s) 408
MaeIII GTNAC 2 cut(s) 55, 244
MalI GATC 3 cut(s) 32, 235, 614
MboI GATC 3 cut(s) 30, 233, 612
MboII GAAGA 5 cut(s) 84, 100, 133, 491, 664
MhlI GDGCHC 1 cut(s) 353
MluCI AATT 1 cut(s) 252
MnlI CCTC 9 cut(s) 21, 143, 195, 250, 285, 392, 519, 610, 668
MseI TTAA 2 cut(s) 255, 332
MspR9I CCNGG 1 cut(s) 575
Mva1269I GAATGC 1 cut(s) 571
MvaI CCWGG 1 cut(s) 575
MwoI GCNNNNNNNGC 3 cut(s) 128, 433, 575
NdeI CATATG 1 cut(s) 97
NdeII GATC 3 cut(s) 30, 233, 612
NlaIII CATG 3 cut(s) 219, 431, 547
NmuCI GTSAC 1 cut(s) 55
NspI RCATGY 1 cut(s) 219
NspV TTCGAA 1 cut(s) 502
PaqCI CACCTGC 1 cut(s) 367
PctI GAATGC 1 cut(s) 571
PfeI GAWTC 4 cut(s) 46, 278, 301, 411
PkrI GCNGC 3 cut(s) 84, 312, 438
Psp6I CCWGG 1 cut(s) 573
PspGI CCWGG 1 cut(s) 573
PspPI GGNCC 1 cut(s) 417
PstI CTGCAG 1 cut(s) 315
RsaI GTAC 3 cut(s) 170, 206, 455
RsaNI GTAC 3 cut(s) 169, 205, 454
SaqAI TTAA 2 cut(s) 255, 332
SatI GCNGC 3 cut(s) 83, 311, 437
Sau3AI GATC 3 cut(s) 30, 233, 612
Sau96I GGNCC 1 cut(s) 417
ScaI AGTACT 2 cut(s) 170, 455
ScrFI CCNGG 1 cut(s) 575
SduI GDGCHC 1 cut(s) 353
SfaNI GCATC 2 cut(s) 109, 331
SfcI CTRYAG 1 cut(s) 311
SfuI TTCGAA 1 cut(s) 502
SinI GGWCC 1 cut(s) 417
SmlI CTYRAG 1 cut(s) 346
SmoI CTYRAG 1 cut(s) 346
Sse9I AATT 1 cut(s) 252
SspMI CTAG 1 cut(s) 408
StyD4I CCNGG 1 cut(s) 573
TaaI ACNGT 1 cut(s) 361
TaqI TCGA 4 cut(s) 33, 502, 522, 615
TasI AATT 1 cut(s) 252
TatI WGTACW 2 cut(s) 168, 453
TfiI GAWTC 4 cut(s) 46, 278, 301, 411
Tru1I TTAA 2 cut(s) 255, 332
Tru9I TTAA 2 cut(s) 255, 332
TseFI GTSAC 1 cut(s) 55
TseI GCWGC 3 cut(s) 82, 310, 436
Tsp45I GTSAC 1 cut(s) 55
TspDTI ATGAA 3 cut(s) 114, 444, 578
VpaK11BI GGWCC 1 cut(s) 417
XceI RCATGY 1 cut(s) 219
XspI CTAG 1 cut(s) 408
ZrmI AGTACT 2 cut(s) 170, 455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.