Rorug07G0256700
MADS Family

Agamous-like MADS-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0003722
Physical Location & Seq
Forward (+)
23998906 .. 24000439
1534 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0256700.1

Sequence Viewer

Length: 903 bp
ATGGCAGAAACAACCATCAGTTTTGGGTCCAAGAGGATTGCTGTTGTTACTGGAGCCAACAAAGGGATTGGACTTGAGATTAGTAGGCAATTAGCTTCTAATGGAGTTGGGGTGGTACTAACAGCAAGAGATGTGAAGAGAGGCACAGAAGCTGTTCAAAAGCTTAAGGCCTCTGGTTTCTCTGATGTGGTCTTTCATCAGCTAGATGTAAATAACCCAACTACCATTGCTTCTCTGGCAGATTTTCTCAAAACTCAGTTTGGAAAGCTTGACATTTTGGTTAACAATGCAGGAGTCCTTGGATCCACATACCTCACCGATAACGTCGTAGTGGATCGTCAAAATGTTTTAGGTTCTACATCACTGAAAGAAGTTTTAGTGCAAACATACGAGACGGCAGAGGATTGCTTGAAAACAAATTATTATGGAATCAAGCAACTCACAGAAGCACTTCTTCCCCTTCTTCAAAAATCAGATGCAGCAAGGATAGTAAATGTCTCTTCAGGACTGGGACAGCTAAGGGTTATTGCAAATGAGAAAGCCAAGAAAGAGCTAGGAGATGTCAATAACCTCACCGAGGAAAAAGTGGACAAGCTGGTTGAGGAATTTCTGGAGGATGTGAAGCAGGATTTGATAGAATCCAAAGGCTGGCCTCTAAACATATCTTCTTACATTGTATCAAAAGCAGCTCTGAATGCCTATACAAGAGTCTTGGCAAAGAAGTATCCTGAAATTGCTACAAACGCAGTTAGTCCTGGCTTTACCAAAACAGATATCAACCAAAATACTGGGATTAACACAGTTGAAGAAGGTGCAGAAGGTCCTGTGAAGCTGGCTTTGATAGCTGACACTGGAATTTCTGGCCTCTACTTTGAAATGACTGAAGAGTCAACCTTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
K-box SRF-TF

Protein Analysis

300

Amino Acids

32.37

Weight (kDa)

5.05

Isoelectric Point (pI)

25.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 13 - 176 1.2e-28 short chain dehydrogenase
KR PF08659 15 - 115 1.6e-08 KR domain
adh_short_C2 PF13561 18 - 178 9.8e-19 Enoyl-(Acyl carrier protein) reductase
adh_short_C2 PF13561 221 - 263 2.7e-06 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 886
AccB7I CCANNNNNTGG 1 cut(s) 648
AclWI GGATC 3 cut(s) 297, 310, 342
AcsI RAATTY 2 cut(s) 605, 855
AcuI CTGAAG 2 cut(s) 486, 903
AfaI GTAC 1 cut(s) 117
AfiI CCNNNNNNNGG 3 cut(s) 63, 577, 648
AflII CTTAAG 1 cut(s) 164
AgsI TTSAA 5 cut(s) 158, 412, 467, 806, 875
AjnI CCWGG 1 cut(s) 754
Alw26I GTCTC 2 cut(s) 386, 502
AlwI GGATC 3 cut(s) 297, 310, 342
AlwNI CAGNNNCTG 1 cut(s) 152
AoxI GGCC 3 cut(s) 168, 650, 862
ApeKI GCWGC 2 cut(s) 479, 686
ApoI RAATTY 2 cut(s) 605, 855
Asp700I GAANNNNTTC 2 cut(s) 153, 450
AspS9I GGNCC 2 cut(s) 27, 821
AsuHPI GGTGA 2 cut(s) 307, 565
AvaII GGWCC 2 cut(s) 27, 821
BamHI GGATCC 1 cut(s) 302
BbvI GCAGC 2 cut(s) 491, 698
BccI CCATC 1 cut(s) 23
BceAI ACGGC 1 cut(s) 411
BciT130I CCWGG 1 cut(s) 756
BciVI GTATCC 1 cut(s) 735
BcoDI GTCTC 2 cut(s) 386, 502
BfaI CTAG 2 cut(s) 203, 554
BfrI CTTAAG 1 cut(s) 164
BfuI GTATCC 1 cut(s) 735
BisI GCNGC 2 cut(s) 480, 687
BlsI GCNGC 2 cut(s) 481, 688
Bme1390I CCNGG 1 cut(s) 756
Bme18I GGWCC 2 cut(s) 27, 821
BmgT120I GGNCC 2 cut(s) 27, 821
BmiI GGNNCC 3 cut(s) 28, 55, 304
BmrFI CCNGG 1 cut(s) 756
BmrI ACTGGG 2 cut(s) 518, 798
BmsI GCATC 1 cut(s) 466
BmuI ACTGGG 2 cut(s) 518, 798
BpmI CTGGAG 2 cut(s) 72, 632
Bpu10I CCTNAGC 1 cut(s) 518
BpuEI CTTGAG 1 cut(s) 95
BsaJI CCNNGG 2 cut(s) 298, 576
BsaXI ACNNNNNCTCC 2 cut(s) 96, 126
Bsc4I CCNNNNNNNGG 3 cut(s) 63, 577, 648
Bse1I ACTGG 4 cut(s) 55, 513, 793, 856
Bse3DI GCAATG 1 cut(s) 225
BseBI CCWGG 1 cut(s) 756
BseDI CCNNGG 2 cut(s) 298, 576
BseGI GGATG 1 cut(s) 622
BseLI CCNNNNNNNGG 3 cut(s) 63, 577, 648
BseMI GCAATG 1 cut(s) 225
BseMII CTCAG 1 cut(s) 269
BseNI ACTGG 4 cut(s) 55, 513, 793, 856
BseXI GCAGC 2 cut(s) 491, 698
BsgI GTGCAG 1 cut(s) 834
BshFI GGCC 3 cut(s) 170, 652, 864
BslFI GGGAC 1 cut(s) 525
BslI CCNNNNNNNGG 3 cut(s) 63, 577, 648
BsmAI GTCTC 2 cut(s) 386, 502
BsmBI CGTCTC 1 cut(s) 386
BsmFI GGGAC 1 cut(s) 525
BsmI GAATGC 1 cut(s) 700
BsnI GGCC 3 cut(s) 170, 652, 864
Bsp143I GATC 2 cut(s) 302, 334
BspANI GGCC 3 cut(s) 170, 652, 864
BspCNI CTCAG 1 cut(s) 268
BspLI GGNNCC 3 cut(s) 28, 55, 304
BspPI GGATC 3 cut(s) 297, 310, 342
BspTI CTTAAG 1 cut(s) 164
BsrDI GCAATG 1 cut(s) 225
BsrI ACTGG 4 cut(s) 55, 513, 793, 856
BssECI CCNNGG 2 cut(s) 298, 576
BssMI GATC 2 cut(s) 302, 334
BssT1I CCWWGG 1 cut(s) 298
Bst2UI CCWGG 1 cut(s) 756
Bst4CI ACNGT 1 cut(s) 802
Bst6I CTCTTC 3 cut(s) 131, 505, 879
BstAFI CTTAAG 1 cut(s) 164
BstC8I GCNNGC 2 cut(s) 650, 834
BstDEI CTNAG 2 cut(s) 255, 518
BstENI CCTNNNNNAGG 1 cut(s) 575
BstF5I GGATG 1 cut(s) 622
BstKTI GATC 2 cut(s) 305, 337
BstMAI GTCTC 2 cut(s) 386, 502
BstMBI GATC 2 cut(s) 302, 334
BstMWI GCNNNNNNNGC 4 cut(s) 236, 695, 743, 842
BstNI CCWGG 1 cut(s) 756
BstSCI CCNGG 1 cut(s) 754
BstV1I GCAGC 2 cut(s) 491, 698
BstX2I RGATCY 1 cut(s) 302
BstXI CCANNNNNNTGG 1 cut(s) 788
BstYI RGATCY 1 cut(s) 302
BsuI GTATCC 1 cut(s) 735
BsuRI GGCC 3 cut(s) 170, 652, 864
BtsCI GGATG 1 cut(s) 622
BtsIMutI CAGTG 2 cut(s) 362, 849
Cac8I GCNNGC 2 cut(s) 650, 834
CaiI CAGNNNCTG 1 cut(s) 152
Cfr13I GGNCC 2 cut(s) 27, 821
Csp6I GTAC 1 cut(s) 116
CviQI GTAC 1 cut(s) 116
DdeI CTNAG 2 cut(s) 255, 518
DpnI GATC 2 cut(s) 304, 336
DpnII GATC 2 cut(s) 302, 334
DrdI GACNNNNNNGTC 1 cut(s) 886
DseDI GACNNNNNNGTC 1 cut(s) 886
Eam1104I CTCTTC 3 cut(s) 131, 505, 879
EarI CTCTTC 3 cut(s) 131, 505, 879
Eco130I CCWWGG 1 cut(s) 298
Eco147I AGGCCT 1 cut(s) 170
Eco32I GATATC 1 cut(s) 775
Eco47I GGWCC 2 cut(s) 27, 821
Eco57I CTGAAG 2 cut(s) 486, 903
EcoNI CCTNNNNNAGG 1 cut(s) 575
EcoO109I RGGNCCY 1 cut(s) 821
EcoRII CCWGG 1 cut(s) 754
EcoRV GATATC 1 cut(s) 775
EcoT14I CCWWGG 1 cut(s) 298
ErhI CCWWGG 1 cut(s) 298
Esp3I CGTCTC 1 cut(s) 386
FaiI YATR 5 cut(s) 310, 388, 426, 662, 702
FalI AAGNNNNNCTT 2 cut(s) 438, 470
FaqI GGGAC 1 cut(s) 525
Fnu4HI GCNGC 2 cut(s) 480, 687
FokI GGATG 1 cut(s) 629
Fsp4HI GCNGC 2 cut(s) 480, 687
FspBI CTAG 2 cut(s) 203, 554
GluI GCNGC 2 cut(s) 480, 687
GsuI CTGGAG 2 cut(s) 72, 632
HaeIII GGCC 3 cut(s) 170, 652, 864
HincII GTYRAC 2 cut(s) 283, 891
HindII GTYRAC 2 cut(s) 283, 891
HindIII AAGCTT 2 cut(s) 161, 266
HinfI GANTC 5 cut(s) 294, 429, 638, 708, 887
HpaI GTTAAC 1 cut(s) 283
HphI GGTGA 2 cut(s) 307, 565
Hpy166II GTNNAC 3 cut(s) 283, 589, 891
Hpy188I TCNGA 3 cut(s) 184, 475, 693
Hpy188III TCNNGA 3 cut(s) 504, 611, 728
Hpy8I GTNNAC 3 cut(s) 283, 589, 891
Hpy99I CGWCG 1 cut(s) 329
HpyAV CCTTC 3 cut(s) 470, 803, 812
HpyCH4III ACNGT 1 cut(s) 802
HpyCH4IV ACGT 1 cut(s) 324
HpyCH4V TGCA 5 cut(s) 290, 382, 479, 530, 815
HpyF10VI GCNNNNNNNGC 4 cut(s) 236, 695, 743, 842
HpyF3I CTNAG 2 cut(s) 255, 518
HpySE526I ACGT 1 cut(s) 324
KspAI GTTAAC 1 cut(s) 283
Kzo9I GATC 2 cut(s) 302, 334
LmnI GCTCC 1 cut(s) 53
Lsp1109I GCAGC 2 cut(s) 491, 698
LweI GCATC 1 cut(s) 466
MaeI CTAG 2 cut(s) 203, 554
MaeII ACGT 1 cut(s) 324
MaeIII GTNAC 1 cut(s) 46
MalI GATC 2 cut(s) 304, 336
MboI GATC 2 cut(s) 302, 334
MboII GAAGA 7 cut(s) 148, 446, 455, 492, 657, 818, 896
MflI RGATCY 1 cut(s) 302
MluCI AATT 5 cut(s) 89, 418, 605, 732, 855
MlyI GAGTC 3 cut(s) 303, 717, 896
MroXI GAANNNNTTC 2 cut(s) 153, 450
MseI TTAA 3 cut(s) 165, 282, 795
MspCI CTTAAG 1 cut(s) 164
MspR9I CCNGG 1 cut(s) 756
Mva1269I GAATGC 1 cut(s) 700
MvaI CCWGG 1 cut(s) 756
MwoI GCNNNNNNNGC 4 cut(s) 236, 695, 743, 842
NdeII GATC 2 cut(s) 302, 334
NlaIV GGNNCC 3 cut(s) 28, 55, 304
PceI AGGCCT 1 cut(s) 170
PctI GAATGC 1 cut(s) 700
PdmI GAANNNNTTC 2 cut(s) 153, 450
PfeI GAWTC 2 cut(s) 429, 638
PflMI CCANNNNNTGG 1 cut(s) 648
PkrI GCNGC 2 cut(s) 481, 688
PleI GAGTC 3 cut(s) 302, 716, 895
PpsI GAGTC 3 cut(s) 302, 716, 895
PpuMI RGGWCCY 1 cut(s) 821
Psp5II RGGWCCY 1 cut(s) 821
Psp6I CCWGG 1 cut(s) 754
PspGI CCWGG 1 cut(s) 754
PspN4I GGNNCC 3 cut(s) 28, 55, 304
PspPI GGNCC 2 cut(s) 27, 821
PspPPI RGGWCCY 1 cut(s) 821
PstNI CAGNNNCTG 1 cut(s) 152
PsuI RGATCY 1 cut(s) 302
RsaI GTAC 1 cut(s) 117
RsaNI GTAC 1 cut(s) 116
SaqAI TTAA 3 cut(s) 165, 282, 795
SatI GCNGC 2 cut(s) 480, 687
Sau3AI GATC 2 cut(s) 302, 334
Sau96I GGNCC 2 cut(s) 27, 821
SchI GAGTC 3 cut(s) 303, 717, 896
ScrFI CCNGG 1 cut(s) 756
SfaNI GCATC 1 cut(s) 466
SinI GGWCC 2 cut(s) 27, 821
SmlI CTYRAG 2 cut(s) 74, 164
SmoI CTYRAG 2 cut(s) 74, 164
Sse9I AATT 5 cut(s) 89, 418, 605, 732, 855
SseBI AGGCCT 1 cut(s) 170
SspMI CTAG 2 cut(s) 203, 554
StuI AGGCCT 1 cut(s) 170
StyD4I CCNGG 1 cut(s) 754
StyI CCWWGG 1 cut(s) 298
TaaI ACNGT 1 cut(s) 802
TaiI ACGT 1 cut(s) 327
TasI AATT 5 cut(s) 89, 418, 605, 732, 855
TfiI GAWTC 2 cut(s) 429, 638
Tru1I TTAA 3 cut(s) 165, 282, 795
Tru9I TTAA 3 cut(s) 165, 282, 795
TscAI CASTG 2 cut(s) 369, 856
TseI GCWGC 2 cut(s) 479, 686
TspDTI ATGAA 1 cut(s) 185
TspRI CASTG 2 cut(s) 369, 856
Van91I CCANNNNNTGG 1 cut(s) 648
Vha464I CTTAAG 1 cut(s) 164
VpaK11BI GGWCC 2 cut(s) 27, 821
XagI CCTNNNNNAGG 1 cut(s) 575
XapI RAATTY 2 cut(s) 605, 855
XcmI CCANNNNNNNNNTGG 1 cut(s) 232
XmnI GAANNNNTTC 2 cut(s) 153, 450
XspI CTAG 2 cut(s) 203, 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.