Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0003522

Physical Location & Seq

Genomic Coordinates Forward (+)

28418540 .. 28426232

Gene Structure 7693 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1221700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1854 bp

ATGGACCCAACACTGATGACGTCCTCCGCCTCTGCTTCTGCATTTTGGCTGATTCTGCTGTTTGGCTTCTTTCACCTCCAGAAGCTTGCTGCCAACGTCGAAGGTGACGCATTGAATGCTTTGAAGAGCAATCTAGCTGATCCAAACAATGTTCTGCAGAGTTGGGATCCGACTCTCGTCAATCCCTGTACCTGGTTTCACGTCACTTGTAATAGCGAAAATAGCGTTACTCGAGTTGATCTGGGCAATGCCAATCTCTCTGGTCAACTGGTGGCACAGCTTGGTGTGCTTTCCAAGTTGCAGTACTTGGAACTTTACAGTAATAACATAACCGGAACCATTCCCCCAGAGCTTGGGGGTTTGTCAAACTTGGTGAGCTTGGATCTTTACTTGAACAATTTACATGGTAACATTCCAGAGACGCTTGGCAACCTTGCAAAACTACGTTTCCTGCGTCTCAACAACAACACCTTGGCGGGGAATATTCCCATGACTTTGACTAAAATCGAATCATTGCAAGTCCTGGATCTTTCAAATAACAATCTGACAGGGGATATTCCTGTCAATGGATCTTTTTCACTTTTTACTCCCATCAGTTTTAACAATAATCCTCTTCTGAAACCACTTCCAGCCTCTCCACCTCCTCCCCTTTCTCCCCCACCAAGTTCTCCAGGCACCACTGCTACTGGGGCTATTGCTGGAGGGGTTGCTGCTGGTGCCGCTTTGCTGTTTGCTGCACCTGCAATTGCACTTGCTTATTGGCGACGAAGAAAACCACAGGATCATTTCTTTGATGTACCTGCTGAGGAGGATCCAGAAGTTCATTTAGGACAGCTCAAAAGGTTTTCTTTGCGTGAGCTACAAGTTGCAACGGATACCTTTAGCAACAAAAATATTCTTGGTAGAGGTGGATTTGGTAAGGTTTATAAAGGACGTTTAACTGATGGAACTCTGGTTGCTGTAAAAAGACTTAAAGAGGAGAGAACCCAAGGCGGGGAGCTACAGTTCCAAACAGAAGTTGAAATGATTAGCATGGCTGTCCACCGCAATCTGCTTCGTCTACGTGGTTTTTGCATGACACCAACAGAAAGGCTGCTCGTATATCCTTATATGGCTAATGGAAGTGTGGCATCATGTTTAAGAGATCGTCCAGAAGATCAAGCTGCACTTGATTGGCCAAAAAGACAATGCATTGCATTGGGTTCTGCAAGAGGCCTTGCTTATTTACATGATCACTGTGATCCAAAAATTATTCACCGTGATGTGAAAGCTGCAAACATATTGTTGGATGAGGAATTTGAAGCAGTTGTTGGAGACTTTGGATTGGCTAAACTCATGGACTACAAGGATACACATGTTACAACAGCTGTACGTGGCACAATTGGTCATATAGCACCAGAGTACCTTTCAACTGGAAAGTCTTCCGAGAAAACTGATGTTTTTGGATACGGAGTCATGCTTCTTGAACTCATCACTGGGCAGAGGGCTTTTGATCTTGCTCGGCTTGCGAATGATGATGATGTCATGTTACTTGATTGGGTCAAAGGACTACTGAAAGATCGGAGGTTGGAAACACTTGTTGATGCTGATCTAAATGGAAATTATGTTGAGGATCAAGTGGAGCAGCTGATCCAAGTAGCTCTACTGTGCACACAAGGCACCCCAGGGGATCGGCCCAAGATGTCAGAGGTTGTCCGAATGCTTGAAGGCGATGGTTTGGCCGAAAGATGGGAGGAGTGGCAGAAAGAGGAGGTGTTCCGCCAAGATTACAACCCTATTCATCATCCAAATACTAATTGGATCATAGACTCAACTTCTCATATTCCTCCGGATGAGTTGTCCGGTCCCAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0008219 GO:0009719 GO:0009725 GO:0009741 GO:0009742 GO:0009755 GO:0009987 GO:0010008 GO:0010033 GO:0010200 GO:0010243 GO:0010941 GO:0012505 GO:0014070 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0031090 GO:0031410 GO:0031982 GO:0032870 GO:0033612 GO:0033993 GO:0036211 GO:0038023 GO:0042221 GO:0042493 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043401 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0048519 GO:0048523 GO:0048545 GO:0048580 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051716 GO:0060089 GO:0060548 GO:0065007 GO:0070887 GO:0071310 GO:0071367 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071944 GO:0097708 GO:0098588 GO:0098805 GO:0140096 GO:1900140 GO:1901564 GO:1901698 GO:1901700 GO:1901701 GO:2000026

KEGG Pathways

Metabolic & Signaling

ko04016 ko04075 ko04626 map04016 map04075 map04626

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

618

Amino Acids

67.93

Weight (kDa)

5.35

Isoelectric Point (pI)

40.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	32 - 71	1.6e-11	Leucine rich repeat N-terminal domain
LRR_14	PF23598	80 - 179	1.9e-10	Leucine-rich repeat region
Pkinase	PF00069	295 - 565	8.4e-43	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	297 - 568	1.1e-44	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003522)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G13790 AT2G13800 AT2G13800 AT2G13800 AT2G13800 AT4G33430 AT4G33430
fragaria_vesca	FvH4_5g30840 FvH4_5g30840
malus_domestica	MD08G1221700.v1.1 MD15G1412700.v1.1 MD15G1413000.v1.1 MD15G1413200.v1.1 MD15G1413300.v1.1 MD15G1414000.v1.1
prunus_persica	Prupe.1G558800_v2.0.a1 Prupe.1G558900_v2.0.a1 Prupe.1G558900_v2.0.a1 Prupe.1G558900_v2.0.a1
pyrus_communis	pycom08g19230 pycom15g36730 pycom15g36740 pycom15g36780 pycom15g36820
rosa_chinensis	RchiOBHm_Chr7g0225981
rosa_laevigata	RLG00000001775
rosa_multiflora	Rmu_sc0002092.1_g000002 Rmu_sc0014767.1_g000001
rosa_roxburghii	Rroxscaffold_3G00233340
rosa_rugosa	Rorug07G0233900
rosa_samantha	Rh7AG369400 Rh7BG367900 Rh7CG387800 Rh7DG378900
rosa_wichuraiana	Rw0G020960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	927
AarI	CACCTGC	1 cut(s)	748
AatII	GACGTC	1 cut(s)	23
Acc36I	ACCTGC	2 cut(s)	748, 808
AccB1I	GGYRCC	3 cut(s)	674, 716, 1658
AccB7I	CCANNNNNTGG	1 cut(s)	353
AccI	GTMKAC	1 cut(s)	1060
AccIII	TCCGGA	1 cut(s)	1828
AciI	CCGC	6 cut(s)	27, 476, 720, 993, 1045, 1759
AcoI	YGGCCR	2 cut(s)	1175, 1719
AcsI	RAATTY	1 cut(s)	1295
AcyI	GRCGYC	1 cut(s)	20
AfaI	GTAC	5 cut(s)	190, 305, 798, 1371, 1403
AfiI	CCNNNNNNNGG	7 cut(s)	192, 353, 477, 566, 993, 994, 1728
AflIII	ACRYGT	1 cut(s)	1354
AgsI	TTSAA	9 cut(s)	115, 124, 394, 534, 1022, 1301, 1410, 1466, 1706
AjiI	CACGTC	1 cut(s)	202
AjnI	CCWGG	4 cut(s)	191, 522, 670, 1663
AjuI	GAANNNNNNNTTGG	2 cut(s)	1293, 1325
AloI	GAACNNNNNNTCC	2 cut(s)	989, 1021
Alw21I	GWGCWC	1 cut(s)	1652
Alw26I	GTCTC	3 cut(s)	413, 461, 1308
Alw44I	GTGCAC	1 cut(s)	1648
Ama87I	CYCGRG	1 cut(s)	231
Aor13HI	TCCGGA	1 cut(s)	1828
AoxI	GGCC	4 cut(s)	1175, 1213, 1673, 1719
ApaLI	GTGCAC	1 cut(s)	1648
ApeKI	GCWGC	7 cut(s)	89, 710, 734, 1093, 1163, 1271, 1624
ApoI	RAATTY	1 cut(s)	1295
Asp700I	GAANNNNTTC	1 cut(s)	1420
AspS9I	GGNCC	3 cut(s)	4, 1674, 1844
AsuHPI	GGTGA	4 cut(s)	65, 116, 385, 1247
AvaI	CYCGRG	1 cut(s)	231
AvaII	GGWCC	2 cut(s)	4, 1844
BaeGI	GKGCMC	1 cut(s)	1652
BaeI	ACNNNNGTAYC	4 cut(s)	1341, 1374, 1385, 1418
BalI	TGGCCA	1 cut(s)	1177
BamHI	GGATCC	2 cut(s)	166, 811
BanI	GGYRCC	3 cut(s)	674, 716, 1658
BbsI	GAAGAC	1 cut(s)	1413
Bbv12I	GWGCWC	1 cut(s)	1652
BbvCI	CCTCAGC	1 cut(s)	804
BbvI	GCAGC	7 cut(s)	76, 697, 721, 1080, 1150, 1258, 1636
BccI	CCATC	4 cut(s)	599, 938, 1706, 1722
BciT130I	CCWGG	4 cut(s)	193, 524, 672, 1665
BciVI	GTATCC	3 cut(s)	868, 1342, 1439
BclI	TGATCA	1 cut(s)	1231
BcoDI	GTCTC	3 cut(s)	413, 461, 1308
BfaI	CTAG	1 cut(s)	134
BfmI	CTRYAG	2 cut(s)	155, 1001
BfuAI	ACCTGC	2 cut(s)	748, 808
BfuI	GTATCC	3 cut(s)	868, 1342, 1439
BisI	GCNGC	8 cut(s)	90, 711, 720, 735, 1094, 1164, 1272, 1625
BlsI	GCNGC	8 cut(s)	91, 712, 721, 736, 1095, 1165, 1273, 1626
BmcAI	AGTACT	1 cut(s)	305
Bme1390I	CCNGG	4 cut(s)	193, 524, 672, 1665
Bme18I	GGWCC	2 cut(s)	4, 1844
BmeT110I	CYCGRG	1 cut(s)	231
BmgBI	CACGTC	1 cut(s)	202
BmgT120I	GGNCC	3 cut(s)	4, 1674, 1844
BmiI	GGNNCC	8 cut(s)	6, 168, 337, 676, 718, 813, 1660, 1846
BmrFI	CCNGG	4 cut(s)	193, 524, 672, 1665
BmrI	ACTGGG	2 cut(s)	696, 1485
BmsI	GCATC	2 cut(s)	1139, 1573
BmuI	ACTGGG	2 cut(s)	696, 1485
BoxI	GACNNNNGTC	1 cut(s)	176
BpiI	GAAGAC	1 cut(s)	1413
BpmI	CTGGAG	3 cut(s)	62, 654, 720
Bpu10I	CCTNAGC	1 cut(s)	804
BsaAI	YACGTR	2 cut(s)	1064, 1373
BsaBI	GATNNNNATC	1 cut(s)	1587
BsaHI	GRCGYC	1 cut(s)	20
BsaJI	CCNNGG	4 cut(s)	471, 988, 1663, 1664
BsaWI	WCCGGW	3 cut(s)	332, 1828, 1841
BsaXI	ACNNNNNCTCC	2 cut(s)	989, 1019
Bsc4I	CCNNNNNNNGG	7 cut(s)	192, 353, 477, 566, 993, 994, 1728
Bse1I	ACTGG	4 cut(s)	273, 691, 1417, 1480
Bse3DI	GCAATG	3 cut(s)	253, 512, 1191
Bse8I	GATNNNNATC	1 cut(s)	1587
BseAI	TCCGGA	1 cut(s)	1828
BseBI	CCWGG	4 cut(s)	193, 524, 672, 1665
BseDI	CCNNGG	4 cut(s)	471, 988, 1663, 1664
BseGI	GGATG	3 cut(s)	1294, 1783, 1837
BseJI	GATNNNNATC	1 cut(s)	1587
BseLI	CCNNNNNNNGG	7 cut(s)	192, 353, 477, 566, 993, 994, 1728
BseMI	GCAATG	3 cut(s)	253, 512, 1191
BseMII	CTCAG	1 cut(s)	795
BseNI	ACTGG	4 cut(s)	273, 691, 1417, 1480
BseRI	GAGGAG	5 cut(s)	633, 821, 992, 1748, 1763
BseSI	GKGCMC	1 cut(s)	1652
BseXI	GCAGC	7 cut(s)	76, 697, 721, 1080, 1150, 1258, 1636
BsgI	GTGCAG	2 cut(s)	720, 1149
BshFI	GGCC	4 cut(s)	1177, 1215, 1675, 1721
BshNI	GGYRCC	3 cut(s)	674, 716, 1658
BsiHKAI	GWGCWC	1 cut(s)	1652
BsiHKCI	CYCGRG	1 cut(s)	231
BsiSI	CCGG	3 cut(s)	333, 1829, 1842
BslFI	GGGAC	1 cut(s)	1830
BslI	CCNNNNNNNGG	7 cut(s)	192, 353, 477, 566, 993, 994, 1728
BsmAI	GTCTC	3 cut(s)	413, 461, 1308
BsmBI	CGTCTC	2 cut(s)	413, 461
BsmFI	GGGAC	1 cut(s)	1830
BsmI	GAATGC	2 cut(s)	121, 1704
BsnI	GGCC	4 cut(s)	1177, 1215, 1675, 1721
BsoBI	CYCGRG	1 cut(s)	231
Bsp1286I	GDGCHC	1 cut(s)	1652
Bsp13I	TCCGGA	1 cut(s)	1828
BspACI	CCGC	6 cut(s)	27, 476, 720, 993, 1045, 1759
BspANI	GGCC	4 cut(s)	1177, 1215, 1675, 1721
BspCNI	CTCAG	1 cut(s)	796
BspEI	TCCGGA	1 cut(s)	1828
BspLI	GGNNCC	8 cut(s)	6, 168, 337, 676, 718, 813, 1660, 1846
BspMAI	CTGCAG	1 cut(s)	159
BspMI	ACCTGC	2 cut(s)	748, 808
BspQI	GCTCTTC	1 cut(s)	119
BspT107I	GGYRCC	3 cut(s)	674, 716, 1658
BsrDI	GCAATG	3 cut(s)	253, 512, 1191
BsrI	ACTGG	4 cut(s)	273, 691, 1417, 1480
BssECI	CCNNGG	4 cut(s)	471, 988, 1663, 1664
BssNI	GRCGYC	1 cut(s)	20
BssT1I	CCWWGG	2 cut(s)	471, 988
Bst2UI	CCWGG	4 cut(s)	193, 524, 672, 1665
Bst4CI	ACNGT	5 cut(s)	320, 1005, 1238, 1259, 1647
Bst6I	CTCTTC	2 cut(s)	119, 618
BstACI	GRCGYC	1 cut(s)	20
BstAPI	GCANNNNNTGC	1 cut(s)	116
BstBAI	YACGTR	2 cut(s)	1064, 1373
BstC8I	GCNNGC	2 cut(s)	87, 1506
BstDEI	CTNAG	1 cut(s)	804
BstF5I	GGATG	3 cut(s)	1294, 1783, 1837
BstMAI	GTCTC	3 cut(s)	413, 461, 1308
BstNI	CCWGG	4 cut(s)	193, 524, 672, 1665
BstNSI	RCATGY	1 cut(s)	1358
BstPAI	GACNNNNGTC	1 cut(s)	176
BstSCI	CCNGG	4 cut(s)	191, 522, 670, 1663
BstSFI	CTRYAG	2 cut(s)	155, 1001
BstSLI	GKGCMC	1 cut(s)	1652
BstV1I	GCAGC	7 cut(s)	76, 697, 721, 1080, 1150, 1258, 1636
BstV2I	GAAGAC	1 cut(s)	1413
BstX2I	RGATCY	5 cut(s)	166, 382, 526, 569, 811
BstYI	RGATCY	5 cut(s)	166, 382, 526, 569, 811
BsuI	GTATCC	3 cut(s)	868, 1342, 1439
BsuRI	GGCC	4 cut(s)	1177, 1215, 1675, 1721
BtgZI	GCGATG	1 cut(s)	1725
BtrI	CACGTC	1 cut(s)	202
BtsCI	GGATG	3 cut(s)	1294, 1783, 1837
BtsI	GCAGTG	1 cut(s)	678
BtsIMutI	CAGTG	4 cut(s)	11, 678, 1234, 1473
BveI	ACCTGC	2 cut(s)	748, 808
Cac8I	GCNNGC	2 cut(s)	87, 1506
Cfr13I	GGNCC	3 cut(s)	4, 1674, 1844
CseI	GACGC	3 cut(s)	116, 430, 443
CsiI	ACCWGGT	1 cut(s)	191
Csp6I	GTAC	5 cut(s)	189, 304, 797, 1370, 1402
CviQI	GTAC	5 cut(s)	189, 304, 797, 1370, 1402
DdeI	CTNAG	1 cut(s)	804
EaeI	YGGCCR	2 cut(s)	1175, 1719
Eam1104I	CTCTTC	2 cut(s)	119, 618
EarI	CTCTTC	2 cut(s)	119, 618
EciI	GGCGGA	2 cut(s)	16, 1748
Eco130I	CCWWGG	2 cut(s)	471, 988
Eco147I	AGGCCT	1 cut(s)	1215
Eco47I	GGWCC	2 cut(s)	4, 1844
Eco88I	CYCGRG	1 cut(s)	231
EcoRII	CCWGG	4 cut(s)	191, 522, 670, 1663
EcoT14I	CCWWGG	2 cut(s)	471, 988
EcoT22I	ATGCAT	1 cut(s)	1193
ErhI	CCWWGG	2 cut(s)	471, 988
Esp3I	CGTCTC	2 cut(s)	413, 461
FalI	AAGNNNNNCTT	4 cut(s)	832, 864, 1152, 1184
FaqI	GGGAC	1 cut(s)	1830
FauI	CCCGC	2 cut(s)	469, 986
FbaI	TGATCA	1 cut(s)	1231
FblI	GTMKAC	1 cut(s)	1060
Fnu4HI	GCNGC	8 cut(s)	90, 711, 720, 735, 1094, 1164, 1272, 1625
FokI	GGATG	3 cut(s)	1301, 1770, 1844
Fsp4HI	GCNGC	8 cut(s)	90, 711, 720, 735, 1094, 1164, 1272, 1625
FspBI	CTAG	1 cut(s)	134
GluI	GCNGC	8 cut(s)	90, 711, 720, 735, 1094, 1164, 1272, 1625
GsuI	CTGGAG	3 cut(s)	62, 654, 720
HaeIII	GGCC	4 cut(s)	1177, 1215, 1675, 1721
HapII	CCGG	3 cut(s)	333, 1829, 1842
HgaI	GACGC	3 cut(s)	116, 430, 443
Hin1I	GRCGYC	1 cut(s)	20
HincII	GTYRAC	1 cut(s)	266
HindII	GTYRAC	1 cut(s)	266
HindIII	AAGCTT	1 cut(s)	83
HinfI	GANTC	5 cut(s)	52, 172, 509, 1452, 1808
HpaII	CCGG	3 cut(s)	333, 1829, 1842
HphI	GGTGA	4 cut(s)	65, 116, 385, 1247
Hpy166II	GTNNAC	4 cut(s)	266, 1042, 1061, 1650
Hpy188I	TCNGA	7 cut(s)	171, 546, 618, 1426, 1563, 1687, 1697
Hpy188III	TCNNGA	6 cut(s)	79, 416, 815, 1151, 1463, 1829
Hpy8I	GTNNAC	4 cut(s)	266, 1042, 1061, 1650
Hpy99I	CGWCG	2 cut(s)	101, 768
HpyAV	CCTTC	2 cut(s)	95, 1700
HpyCH4III	ACNGT	5 cut(s)	320, 1005, 1238, 1259, 1647
HpyCH4IV	ACGT	7 cut(s)	20, 96, 201, 445, 934, 1063, 1372
HpyF3I	CTNAG	1 cut(s)	804
HpySE526I	ACGT	7 cut(s)	20, 96, 201, 445, 934, 1063, 1372
Hsp92I	GRCGYC	1 cut(s)	20
Kpn2I	TCCGGA	1 cut(s)	1828
Ksp22I	TGATCA	1 cut(s)	1231
LguI	GCTCTTC	1 cut(s)	119
LmnI	GCTCC	2 cut(s)	997, 1621
Lsp1109I	GCAGC	7 cut(s)	76, 697, 721, 1080, 1150, 1258, 1636
LweI	GCATC	2 cut(s)	1139, 1573
MabI	ACCWGGT	1 cut(s)	191
MaeI	CTAG	1 cut(s)	134
MaeII	ACGT	7 cut(s)	20, 96, 201, 445, 934, 1063, 1372
MaeIII	GTNAC	6 cut(s)	104, 202, 226, 407, 1357, 1527
MboII	GAAGA	5 cut(s)	136, 605, 780, 1166, 1413
MfeI	CAATTG	2 cut(s)	744, 1380
MflI	RGATCY	5 cut(s)	166, 382, 526, 569, 811
MhlI	GDGCHC	1 cut(s)	1652
MlsI	TGGCCA	1 cut(s)	1177
MluCI	AATT	7 cut(s)	397, 744, 1248, 1295, 1380, 1600, 1795
MluNI	TGGCCA	1 cut(s)	1177
MlyI	GAGTC	3 cut(s)	166, 1461, 1802
MmeI	TCCRAC	4 cut(s)	194, 1266, 1291, 1548
Mox20I	TGGCCA	1 cut(s)	1177
Mph1103I	ATGCAT	1 cut(s)	1193
MroI	TCCGGA	1 cut(s)	1828
MroXI	GAANNNNTTC	1 cut(s)	1420
MscI	TGGCCA	1 cut(s)	1177
MseI	TTAA	4 cut(s)	600, 938, 972, 1139
MslI	CAYNNNNRTG	1 cut(s)	1260
Msp20I	TGGCCA	1 cut(s)	1177
MspA1I	CMGCKG	2 cut(s)	1367, 1627
MspI	CCGG	3 cut(s)	333, 1829, 1842
MspR9I	CCNGG	4 cut(s)	193, 524, 672, 1665
MunI	CAATTG	2 cut(s)	744, 1380
Mva1269I	GAATGC	2 cut(s)	121, 1704
MvaI	CCWGG	4 cut(s)	193, 524, 672, 1665
NlaIV	GGNNCC	8 cut(s)	6, 168, 337, 676, 718, 813, 1660, 1846
NmeAIII	GCCGAG	1 cut(s)	1480
NmuCI	GTSAC	2 cut(s)	104, 202
NsiI	ATGCAT	1 cut(s)	1193
NspI	RCATGY	1 cut(s)	1358
PaeR7I	CTCGAG	1 cut(s)	231
PaqCI	CACCTGC	1 cut(s)	748
PasI	CCCWGGG	1 cut(s)	1664
PceI	AGGCCT	1 cut(s)	1215
PciI	ACATGT	1 cut(s)	1354
PciSI	GCTCTTC	1 cut(s)	119
PcsI	WCGNNNNNNNCGW	1 cut(s)	451
PctI	GAATGC	2 cut(s)	121, 1704
PdmI	GAANNNNTTC	1 cut(s)	1420
PfeI	GAWTC	2 cut(s)	52, 509
PflMI	CCANNNNNTGG	1 cut(s)	353
PfoI	TCCNGGA	1 cut(s)	522
PkrI	GCNGC	8 cut(s)	91, 712, 721, 736, 1095, 1165, 1273, 1626
PleI	GAGTC	3 cut(s)	166, 1460, 1802
PpsI	GAGTC	3 cut(s)	166, 1460, 1802
Ppu21I	YACGTR	2 cut(s)	1064, 1373
PscI	ACATGT	1 cut(s)	1354
PshAI	GACNNNNGTC	1 cut(s)	176
PsiI	TTATAA	1 cut(s)	927
Psp6I	CCWGG	4 cut(s)	191, 522, 670, 1663
PspGI	CCWGG	4 cut(s)	191, 522, 670, 1663
PspN4I	GGNNCC	8 cut(s)	6, 168, 337, 676, 718, 813, 1660, 1846
PspPI	GGNCC	3 cut(s)	4, 1674, 1844
PspXI	VCTCGAGB	1 cut(s)	231
PstI	CTGCAG	1 cut(s)	159
PsuI	RGATCY	5 cut(s)	166, 382, 526, 569, 811
PvuII	CAGCTG	2 cut(s)	1367, 1627
RsaI	GTAC	5 cut(s)	190, 305, 798, 1371, 1403
RsaNI	GTAC	5 cut(s)	189, 304, 797, 1370, 1402
RseI	CAYNNNNRTG	1 cut(s)	1260
SapI	GCTCTTC	1 cut(s)	119
SaqAI	TTAA	4 cut(s)	600, 938, 972, 1139
SatI	GCNGC	8 cut(s)	90, 711, 720, 735, 1094, 1164, 1272, 1625
Sau96I	GGNCC	3 cut(s)	4, 1674, 1844
ScaI	AGTACT	1 cut(s)	305
SchI	GAGTC	3 cut(s)	166, 1461, 1802
ScrFI	CCNGG	4 cut(s)	193, 524, 672, 1665
SduI	GDGCHC	1 cut(s)	1652
SexAI	ACCWGGT	1 cut(s)	191
SfaNI	GCATC	2 cut(s)	1139, 1573
SfcI	CTRYAG	2 cut(s)	155, 1001
Sfr274I	CTCGAG	1 cut(s)	231
SinI	GGWCC	2 cut(s)	4, 1844
SlaI	CTCGAG	1 cut(s)	231
SmiMI	CAYNNNNRTG	1 cut(s)	1260
SmlI	CTYRAG	1 cut(s)	231
SmoI	CTYRAG	1 cut(s)	231
Sse9I	AATT	7 cut(s)	397, 744, 1248, 1295, 1380, 1600, 1795
SseBI	AGGCCT	1 cut(s)	1215
SsiI	CCGC	6 cut(s)	27, 476, 720, 993, 1045, 1759
SspI	AATATT	2 cut(s)	484, 895
SspMI	CTAG	1 cut(s)	134
StuI	AGGCCT	1 cut(s)	1215
StyD4I	CCNGG	4 cut(s)	191, 522, 670, 1663
StyI	CCWWGG	2 cut(s)	471, 988
TaaI	ACNGT	5 cut(s)	320, 1005, 1238, 1259, 1647
TaiI	ACGT	7 cut(s)	23, 99, 204, 448, 937, 1066, 1375
TaqI	TCGA	3 cut(s)	99, 232, 507
TasI	AATT	7 cut(s)	397, 744, 1248, 1295, 1380, 1600, 1795
TatI	WGTACW	1 cut(s)	303
TauI	GCSGC	1 cut(s)	722
TfiI	GAWTC	2 cut(s)	52, 509
Tru1I	TTAA	4 cut(s)	600, 938, 972, 1139
Tru9I	TTAA	4 cut(s)	600, 938, 972, 1139
TscAI	CASTG	4 cut(s)	18, 685, 1241, 1480
TseFI	GTSAC	2 cut(s)	104, 202
TseI	GCWGC	7 cut(s)	89, 710, 734, 1093, 1163, 1271, 1624
Tsp45I	GTSAC	2 cut(s)	104, 202
TspDTI	ATGAA	2 cut(s)	812, 1769
TspGWI	ACGGA	2 cut(s)	887, 1464
TspRI	CASTG	4 cut(s)	18, 685, 1241, 1480
Van91I	CCANNNNNTGG	1 cut(s)	353
VneI	GTGCAC	1 cut(s)	1648
VpaK11BI	GGWCC	2 cut(s)	4, 1844
XapI	RAATTY	1 cut(s)	1295
XceI	RCATGY	1 cut(s)	1358
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1794
XhoI	CTCGAG	1 cut(s)	231
XmiI	GTMKAC	1 cut(s)	1060
XmnI	GAANNNNTTC	1 cut(s)	1420
XspI	CTAG	1 cut(s)	134
ZraI	GACGTC	1 cut(s)	21
ZrmI	AGTACT	1 cut(s)	305
Zsp2I	ATGCAT	1 cut(s)	1193

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.