Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0007862

Physical Location & Seq

Genomic Coordinates Reverse (-)

29475090 .. 29480228

Gene Structure 5139 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1228600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1413 bp

ATGGGGAAGGGTGAAGCAAATCTGCACCAGCAGCAGCCGAGTCATGAGGGTCAGAGTGCTTCGGGACTCATCTGTCCTGGATGTTCGATGGTTTTCAACCGATTCGCCAAAGGCTTGAGCTTTAGATGCGTCTTCGTTTTAGTTCTCAGTTTATCGGTTTTTCTCTCTGGGATCTTTTGGATCCTTCCTCACCGCGCTACCAACTCGGGGTTTGATGCCACACAGGCAATTAAGCTCAGTGCCACAGTTCAGGCATACTTCAGACTGGAAAAGCCAGTTACGGACCTTGTTCCACACATTGGAAGATTAGAATATGATATCAATGGGGAAATAGGTGTCCCAGGTACAAAGGTGGCTATCTTGTCTATGCACCAATTTCCTGCACATAATTGGACAGATGTGGTGTTTGGTTTTCTTTCTGATCCAATAAATGTTCCAATAGTTCCTGTGTCCTTAAGTGTGCTGAGGTCATCCTTAGTTGAGCTTTTCCTTAAGCAATCCAATCTTACACTGACAACATCAATTTTCGGGCAGCCATCTACATTGGAGATTCTGAAGTATCCGGGGGGAATCACTGTGATACCTGGTCAACCTGCTTCAATCTGGCAGTTACCCGAGATTTTATTTAATTTTACTCTCAATAATTATATAGATGATATAGTGGAAAATTTTGGCGAGTTAAAGGAGCAGTTGAAGTTTGGATTGTATCTGAGGCCTTATGAGAATGTATATTTGCAAATAACAAACACAATGGGTTCAACAACAGCAGCCCCAGTTGTAGTTCAAGCTTCCCTAATGTCTGAGTTTGGAGGCTTTGGGCCCCAGAGATTAAGGCAGTTGGCTCAAATAATCACGGGTTCTCCCACAAAAAATCTTGGCCTGGATAACTCAGTGTTTGGTAAAATTAAAAGTATCAGTTTATCTTCTTATCTGAAAGGTACACTCTCCGCAACCCCTCCAACTGTTTCTCCAGCTCCAACTCCAGAGCCATCAATTTCTCCGTATCTTGCTTCTCCAGTTTACGCTCCAGCACCCTCACCTGACATTCATCATCTCTCATCAGCACCATCCAAGGTACCTGCACATCCTCATCCATGTCCGCACCAAGGTTCTAGAATTCCTCCATCCTCCCCACCAACTTCTCGTTCTTACCCTACTGTTCCTCCTACATATCCTCCTAGAATTCCTCCATCCTCCTCACCACCTTCATCTCAATTGTCCCCACATGTTTCACCTGCTCCTGTAGTGTCTTATGCTCCCAGTCCAGAAGACAAGGGAATTGCACAAGACTTGATATCTCCATCACCTGGCCCATCCCTGTCGTCTTTAGCAGTAGGACCTTTGTACAAGGAGATCCGGTTATTGCAACTTTCAGGAGTCTTCATCTTTCTTCTCTTCTGTTGGTCACGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

471

Amino Acids

50.65

Weight (kDa)

8.49

Isoelectric Point (pI)

59.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF7036	PF23041	86 - 177	2.2e-28	Domain of unknown function (DUF7036)
DUF7036	PF23041	210 - 300	4.7e-19	Domain of unknown function (DUF7036)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007862)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10790
fragaria_vesca	FvH4_5g29560 FvH4_5g29560
malus_domestica	MD08G1228500.v1.1 MD08G1228600.v1.1 MD15G1422400.v1.1 MD15G1422500.v1.1
prunus_persica	Prupe.1G567800_v2.0.a1
pyrus_communis	pycom08g19900 pycom08g19910 pycom15g37430 pycom15g37440
rosa_chinensis	RchiOBHm_Chr7g0222521
rosa_laevigata	RLG00000002048
rosa_multiflora	Rmu_ssc0000146.1_g000026 Rmu_ssc0000385.1_g000001
rosa_roxburghii	Rroxscaffold_3G00236690
rosa_rugosa	Rorug07G0210200
rosa_samantha	Rh7AG351900 Rh7BG342500 Rh7CG369300 Rh7DG348600
rosa_wichuraiana	Rw7G029710 Rw7G030030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1243
AasI	GACNNNNNNGTC	1 cut(s)	72
Acc36I	ACCTGC	3 cut(s)	601, 1087, 1243
Acc65I	GGTACC	1 cut(s)	1075
AccB1I	GGYRCC	1 cut(s)	1075
AccB7I	CCANNNNNTGG	2 cut(s)	299, 1307
AccII	CGCG	1 cut(s)	195
AciI	CCGC	3 cut(s)	193, 948, 1100
AclWI	GGATC	5 cut(s)	175, 179, 188, 416, 1348
AcsI	RAATTY	3 cut(s)	667, 1116, 1182
AcuI	CTGAAG	2 cut(s)	244, 575
AfaI	GTAC	4 cut(s)	346, 940, 1077, 1346
AfiI	CCNNNNNNNGG	4 cut(s)	207, 299, 1106, 1307
AflII	CTTAAG	2 cut(s)	454, 491
AflIII	ACRYGT	1 cut(s)	1225
AgsI	TTSAA	5 cut(s)	97, 600, 694, 759, 785
AjnI	CCWGG	5 cut(s)	76, 340, 583, 879, 1306
AjuI	GAANNNNNNNTTGG	2 cut(s)	1129, 1161
AluBI	AGCT	5 cut(s)	120, 235, 484, 788, 974
AluI	AGCT	5 cut(s)	120, 235, 484, 788, 974
AlwI	GGATC	5 cut(s)	175, 179, 188, 416, 1348
Ama87I	CYCGRG	2 cut(s)	205, 614
AoxI	GGCC	4 cut(s)	713, 818, 877, 1309
ApaI	GGGCCC	1 cut(s)	822
ApeKI	GCWGC	4 cut(s)	31, 34, 532, 767
ApoI	RAATTY	3 cut(s)	667, 1116, 1182
Asp718I	GGTACC	1 cut(s)	1075
AspLEI	GCGC	1 cut(s)	197
AspS9I	GGNCC	5 cut(s)	283, 818, 819, 1310, 1337
AsuC2I	CCSGG	1 cut(s)	564
AsuHPI	GGTGA	6 cut(s)	23, 182, 1029, 1191, 1224, 1296
AvaI	CYCGRG	2 cut(s)	205, 614
AvaII	GGWCC	2 cut(s)	283, 1337
BaeGI	GKGCMC	1 cut(s)	822
BaeI	ACNNNNGTAYC	2 cut(s)	336, 369
BamHI	GGATCC	1 cut(s)	180
BanI	GGYRCC	1 cut(s)	1075
BanII	GRGCYC	1 cut(s)	822
BbsI	GAAGAC	3 cut(s)	124, 1275, 1372
BbvCI	CCTCAGC	1 cut(s)	464
BbvI	GCAGC	4 cut(s)	43, 46, 544, 779
BccI	CCATC	8 cut(s)	82, 544, 997, 1075, 1132, 1198, 1309, 1321
BciT130I	CCWGG	5 cut(s)	78, 342, 585, 881, 1308
BciVI	GTATCC	1 cut(s)	570
BcnI	CCSGG	1 cut(s)	564
BfaI	CTAG	2 cut(s)	1113, 1179
BfmI	CTRYAG	1 cut(s)	1242
BfrI	CTTAAG	2 cut(s)	454, 491
BfuAI	ACCTGC	3 cut(s)	601, 1087, 1243
BfuI	GTATCC	1 cut(s)	570
BglI	GCCNNNNNGGC	1 cut(s)	224
BisI	GCNGC	4 cut(s)	32, 35, 533, 768
BlsI	GCNGC	4 cut(s)	33, 36, 534, 769
Bme1390I	CCNGG	6 cut(s)	78, 342, 564, 585, 881, 1308
Bme18I	GGWCC	2 cut(s)	283, 1337
BmeT110I	CYCGRG	2 cut(s)	205, 614
BmgT120I	GGNCC	5 cut(s)	283, 818, 819, 1310, 1337
BmiI	GGNNCC	4 cut(s)	182, 820, 821, 1077
BmrFI	CCNGG	6 cut(s)	78, 342, 564, 585, 881, 1308
BmrI	ACTGGG	2 cut(s)	767, 1254
BmsI	GCATC	2 cut(s)	116, 205
BmuI	ACTGGG	2 cut(s)	767, 1254
BpiI	GAAGAC	3 cut(s)	124, 1275, 1372
BpmI	CTGGAG	4 cut(s)	954, 966, 999, 1011
Bpu10I	CCTNAGC	1 cut(s)	464
BpuEI	CTTGAG	1 cut(s)	136
BpuMI	CCSGG	1 cut(s)	564
BsaJI	CCNNGG	4 cut(s)	340, 563, 1071, 1105
BsaWI	WCCGGW	1 cut(s)	1356
Bsc4I	CCNNNNNNNGG	4 cut(s)	207, 299, 1106, 1307
Bse1I	ACTGG	5 cut(s)	270, 275, 773, 1016, 1260
BseBI	CCWGG	5 cut(s)	78, 342, 585, 881, 1308
BseDI	CCNNGG	4 cut(s)	340, 563, 1071, 1105
BseGI	GGATG	8 cut(s)	86, 470, 1067, 1084, 1090, 1124, 1190, 1313
BseLI	CCNNNNNNNGG	4 cut(s)	207, 299, 1106, 1307
BseMII	CTCAG	6 cut(s)	160, 250, 455, 701, 792, 903
BseNI	ACTGG	5 cut(s)	270, 275, 773, 1016, 1260
BseRI	GAGGAG	1 cut(s)	1186
BseSI	GKGCMC	1 cut(s)	822
BseXI	GCAGC	4 cut(s)	43, 46, 544, 779
BsgI	GTGCAG	3 cut(s)	8, 366, 1065
Bsh1236I	CGCG	1 cut(s)	195
BshFI	GGCC	4 cut(s)	715, 820, 879, 1311
BshNI	GGYRCC	1 cut(s)	1075
BsiHKCI	CYCGRG	2 cut(s)	205, 614
BsiSI	CCGG	2 cut(s)	563, 1357
BslFI	GGGAC	3 cut(s)	78, 323, 1204
BslI	CCNNNNNNNGG	4 cut(s)	207, 299, 1106, 1307
BsmFI	GGGAC	3 cut(s)	78, 323, 1204
BsnI	GGCC	4 cut(s)	715, 820, 879, 1311
BsoBI	CYCGRG	2 cut(s)	205, 614
Bsp120I	GGGCCC	1 cut(s)	818
Bsp1286I	GDGCHC	1 cut(s)	822
Bsp1407I	TGTACA	1 cut(s)	1344
Bsp143I	GATC	4 cut(s)	171, 180, 421, 1353
BspACI	CCGC	3 cut(s)	193, 948, 1100
BspANI	GGCC	4 cut(s)	715, 820, 879, 1311
BspCNI	CTCAG	6 cut(s)	159, 249, 456, 702, 793, 902
BspFNI	CGCG	1 cut(s)	195
BspHI	TCATGA	1 cut(s)	43
BspLI	GGNNCC	4 cut(s)	182, 820, 821, 1077
BspMI	ACCTGC	3 cut(s)	601, 1087, 1243
BspPI	GGATC	5 cut(s)	175, 179, 188, 416, 1348
BspT107I	GGYRCC	1 cut(s)	1075
BspTI	CTTAAG	2 cut(s)	454, 491
BsrGI	TGTACA	1 cut(s)	1344
BsrI	ACTGG	5 cut(s)	270, 275, 773, 1016, 1260
BssECI	CCNNGG	4 cut(s)	340, 563, 1071, 1105
BssMI	GATC	4 cut(s)	171, 180, 421, 1353
BssT1I	CCWWGG	2 cut(s)	1071, 1105
Bst2UI	CCWGG	5 cut(s)	78, 342, 585, 881, 1308
Bst4CI	ACNGT	5 cut(s)	247, 577, 964, 1159, 1410
Bst6I	CTCTTC	1 cut(s)	1400
BstAFI	CTTAAG	2 cut(s)	454, 491
BstAUI	TGTACA	1 cut(s)	1344
BstDEI	CTNAG	7 cut(s)	146, 236, 464, 475, 710, 801, 889
BstF5I	GGATG	8 cut(s)	86, 470, 1067, 1084, 1090, 1124, 1190, 1313
BstFNI	CGCG	1 cut(s)	195
BstHHI	GCGC	1 cut(s)	197
BstKTI	GATC	4 cut(s)	174, 183, 424, 1356
BstMBI	GATC	4 cut(s)	171, 180, 421, 1353
BstMWI	GCNNNNNNNGC	3 cut(s)	31, 126, 224
BstNI	CCWGG	5 cut(s)	78, 342, 585, 881, 1308
BstNSI	RCATGY	1 cut(s)	1229
BstSCI	CCNGG	6 cut(s)	76, 340, 562, 583, 879, 1306
BstSFI	CTRYAG	1 cut(s)	1242
BstSLI	GKGCMC	1 cut(s)	822
BstUI	CGCG	1 cut(s)	195
BstV1I	GCAGC	4 cut(s)	43, 46, 544, 779
BstV2I	GAAGAC	3 cut(s)	124, 1275, 1372
BstX2I	RGATCY	3 cut(s)	171, 180, 1353
BstYI	RGATCY	3 cut(s)	171, 180, 1353
BsuI	GTATCC	1 cut(s)	570
BsuRI	GGCC	4 cut(s)	715, 820, 879, 1311
BtsCI	GGATG	8 cut(s)	86, 470, 1067, 1084, 1090, 1124, 1190, 1313
BtsIMutI	CAGTG	4 cut(s)	244, 509, 573, 897
BveI	ACCTGC	3 cut(s)	601, 1087, 1243
CciI	TCATGA	1 cut(s)	43
CfoI	GCGC	1 cut(s)	197
Cfr13I	GGNCC	5 cut(s)	283, 818, 819, 1310, 1337
CseI	GACGC	1 cut(s)	118
CsiI	ACCWGGT	1 cut(s)	583
Csp6I	GTAC	4 cut(s)	345, 939, 1076, 1345
CviAII	CATG	3 cut(s)	44, 1095, 1226
CviQI	GTAC	4 cut(s)	345, 939, 1076, 1345
DdeI	CTNAG	7 cut(s)	146, 236, 464, 475, 710, 801, 889
DpnI	GATC	4 cut(s)	173, 182, 423, 1355
DpnII	GATC	4 cut(s)	171, 180, 421, 1353
DrdI	GACNNNNNNGTC	1 cut(s)	72
DseDI	GACNNNNNNGTC	1 cut(s)	72
Eam1104I	CTCTTC	1 cut(s)	1400
EarI	CTCTTC	1 cut(s)	1400
Eco130I	CCWWGG	2 cut(s)	1071, 1105
Eco147I	AGGCCT	1 cut(s)	715
Eco24I	GRGCYC	1 cut(s)	822
Eco32I	GATATC	2 cut(s)	319, 1296
Eco47I	GGWCC	2 cut(s)	283, 1337
Eco57I	CTGAAG	2 cut(s)	244, 575
Eco88I	CYCGRG	2 cut(s)	205, 614
EcoO109I	RGGNCCY	2 cut(s)	819, 1337
EcoRI	GAATTC	2 cut(s)	1116, 1182
EcoRII	CCWGG	5 cut(s)	76, 340, 583, 879, 1306
EcoRV	GATATC	2 cut(s)	319, 1296
EcoT14I	CCWWGG	2 cut(s)	1071, 1105
EcoT38I	GRGCYC	1 cut(s)	822
ErhI	CCWWGG	2 cut(s)	1071, 1105
FaeI	CATG	3 cut(s)	47, 1098, 1229
FaqI	GGGAC	3 cut(s)	78, 323, 1204
FatI	CATG	3 cut(s)	43, 1094, 1225
Fnu4HI	GCNGC	4 cut(s)	32, 35, 533, 768
FokI	GGATG	8 cut(s)	93, 457, 1054, 1071, 1077, 1111, 1177, 1300
FriOI	GRGCYC	1 cut(s)	822
Fsp4HI	GCNGC	4 cut(s)	32, 35, 533, 768
FspBI	CTAG	2 cut(s)	1113, 1179
GlaI	GCGC	1 cut(s)	196
GluI	GCNGC	4 cut(s)	32, 35, 533, 768
GsuI	CTGGAG	4 cut(s)	954, 966, 999, 1011
HaeIII	GGCC	4 cut(s)	715, 820, 879, 1311
HapII	CCGG	2 cut(s)	563, 1357
HgaI	GACGC	1 cut(s)	118
HhaI	GCGC	1 cut(s)	197
Hin1II	CATG	3 cut(s)	47, 1098, 1229
Hin6I	GCGC	1 cut(s)	195
HinP1I	GCGC	1 cut(s)	195
HincII	GTYRAC	1 cut(s)	590
HindII	GTYRAC	1 cut(s)	590
HindIII	AAGCTT	1 cut(s)	786
HinfI	GANTC	6 cut(s)	40, 66, 102, 550, 570, 1377
HpaII	CCGG	2 cut(s)	563, 1357
HphI	GGTGA	6 cut(s)	23, 182, 1029, 1191, 1224, 1296
Hpy166II	GTNNAC	3 cut(s)	590, 941, 1021
Hpy188I	TCNGA	7 cut(s)	54, 263, 421, 555, 711, 802, 933
Hpy188III	TCNNGA	6 cut(s)	44, 63, 983, 1113, 1265, 1374
Hpy8I	GTNNAC	3 cut(s)	590, 941, 1021
HpyAV	CCTTC	2 cut(s)	194, 1215
HpyCH4III	ACNGT	5 cut(s)	247, 577, 964, 1159, 1410
HpyCH4V	TGCA	7 cut(s)	25, 370, 383, 736, 1082, 1283, 1366
HpyF10VI	GCNNNNNNNGC	3 cut(s)	31, 126, 224
HpyF3I	CTNAG	7 cut(s)	146, 236, 464, 475, 710, 801, 889
Hsp92II	CATG	3 cut(s)	47, 1098, 1229
HspAI	GCGC	1 cut(s)	195
KpnI	GGTACC	1 cut(s)	1079
Kzo9I	GATC	4 cut(s)	171, 180, 421, 1353
LmnI	GCTCC	5 cut(s)	685, 979, 1030, 1243, 1261
Lsp1109I	GCAGC	4 cut(s)	43, 46, 544, 779
LweI	GCATC	2 cut(s)	116, 205
MabI	ACCWGGT	1 cut(s)	583
MaeI	CTAG	2 cut(s)	1113, 1179
MaeIII	GTNAC	3 cut(s)	277, 609, 1404
MalI	GATC	4 cut(s)	173, 182, 423, 1355
MboI	GATC	4 cut(s)	171, 180, 421, 1353
MboII	GAAGA	7 cut(s)	124, 315, 915, 1280, 1372, 1382, 1387
MfeI	CAATTG	1 cut(s)	1214
MflI	RGATCY	3 cut(s)	171, 180, 1353
MhlI	GDGCHC	1 cut(s)	822
MlyI	GAGTC	3 cut(s)	49, 60, 1386
MmeI	TCCRAC	2 cut(s)	983, 1001
MseI	TTAA	7 cut(s)	231, 455, 492, 627, 680, 830, 906
MspCI	CTTAAG	2 cut(s)	454, 491
MspI	CCGG	2 cut(s)	563, 1357
MspR9I	CCNGG	6 cut(s)	78, 342, 564, 585, 881, 1308
MunI	CAATTG	1 cut(s)	1214
MvaI	CCWGG	5 cut(s)	78, 342, 585, 881, 1308
MvnI	CGCG	1 cut(s)	195
MwoI	GCNNNNNNNGC	3 cut(s)	31, 126, 224
NciI	CCSGG	1 cut(s)	564
NdeII	GATC	4 cut(s)	171, 180, 421, 1353
NlaIII	CATG	3 cut(s)	47, 1098, 1229
NlaIV	GGNNCC	4 cut(s)	182, 820, 821, 1077
NmeAIII	GCCGAG	1 cut(s)	63
NmuCI	GTSAC	1 cut(s)	1404
NspI	RCATGY	1 cut(s)	1229
PagI	TCATGA	1 cut(s)	43
PaqCI	CACCTGC	1 cut(s)	1243
PceI	AGGCCT	1 cut(s)	715
PciI	ACATGT	1 cut(s)	1225
PfeI	GAWTC	3 cut(s)	102, 550, 570
PflMI	CCANNNNNTGG	2 cut(s)	299, 1307
PfoI	TCCNGGA	1 cut(s)	76
PkrI	GCNGC	4 cut(s)	33, 36, 534, 769
PleI	GAGTC	3 cut(s)	48, 60, 1385
PpsI	GAGTC	3 cut(s)	48, 60, 1385
PpuMI	RGGWCCY	1 cut(s)	1337
PscI	ACATGT	1 cut(s)	1225
Psp5II	RGGWCCY	1 cut(s)	1337
Psp6I	CCWGG	5 cut(s)	76, 340, 583, 879, 1306
PspGI	CCWGG	5 cut(s)	76, 340, 583, 879, 1306
PspN4I	GGNNCC	4 cut(s)	182, 820, 821, 1077
PspOMI	GGGCCC	1 cut(s)	818
PspPI	GGNCC	5 cut(s)	283, 818, 819, 1310, 1337
PspPPI	RGGWCCY	1 cut(s)	1337
PsuI	RGATCY	3 cut(s)	171, 180, 1353
RsaI	GTAC	4 cut(s)	346, 940, 1077, 1346
RsaNI	GTAC	4 cut(s)	345, 939, 1076, 1345
SaqAI	TTAA	7 cut(s)	231, 455, 492, 627, 680, 830, 906
SatI	GCNGC	4 cut(s)	32, 35, 533, 768
Sau3AI	GATC	4 cut(s)	171, 180, 421, 1353
Sau96I	GGNCC	5 cut(s)	283, 818, 819, 1310, 1337
SchI	GAGTC	3 cut(s)	49, 60, 1386
ScrFI	CCNGG	6 cut(s)	78, 342, 564, 585, 881, 1308
SduI	GDGCHC	1 cut(s)	822
SexAI	ACCWGGT	1 cut(s)	583
SfaNI	GCATC	2 cut(s)	116, 205
SfcI	CTRYAG	1 cut(s)	1242
SinI	GGWCC	2 cut(s)	283, 1337
SmlI	CTYRAG	3 cut(s)	115, 454, 491
SmoI	CTYRAG	3 cut(s)	115, 454, 491
SseBI	AGGCCT	1 cut(s)	715
SsiI	CCGC	3 cut(s)	193, 948, 1100
SspMI	CTAG	2 cut(s)	1113, 1179
StuI	AGGCCT	1 cut(s)	715
StyD4I	CCNGG	6 cut(s)	76, 340, 562, 583, 879, 1306
StyI	CCWWGG	2 cut(s)	1071, 1105
TaaI	ACNGT	5 cut(s)	247, 577, 964, 1159, 1410
TaqI	TCGA	1 cut(s)	86
TatI	WGTACW	1 cut(s)	1344
TfiI	GAWTC	3 cut(s)	102, 550, 570
Tru1I	TTAA	7 cut(s)	231, 455, 492, 627, 680, 830, 906
Tru9I	TTAA	7 cut(s)	231, 455, 492, 627, 680, 830, 906
TscAI	CASTG	4 cut(s)	244, 516, 580, 897
TseFI	GTSAC	1 cut(s)	1404
TseI	GCWGC	4 cut(s)	31, 34, 532, 767
Tsp45I	GTSAC	1 cut(s)	1404
TspDTI	ATGAA	3 cut(s)	1037, 1197, 1372
TspGWI	ACGGA	2 cut(s)	296, 990
TspRI	CASTG	4 cut(s)	244, 516, 580, 897
Van91I	CCANNNNNTGG	2 cut(s)	299, 1307
Vha464I	CTTAAG	2 cut(s)	454, 491
VpaK11BI	GGWCC	2 cut(s)	283, 1337
XapI	RAATTY	3 cut(s)	667, 1116, 1182
XbaI	TCTAGA	1 cut(s)	1112
XceI	RCATGY	1 cut(s)	1229
XspI	CTAG	2 cut(s)	1113, 1179

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.