MD09G1007400.v1.1

Glutaredoxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0004777
Physical Location & Seq
Reverse (-)
524570 .. 524950
381 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1007400.v1.1.491

Sequence Viewer

Length: 381 bp
ATGCACTACCAGACCGAGTCATGGGGCTCCTGCATGCCCACAAGAACCTCCATGGGCATAGAGGACCCTCTGGAGCGCATAGAGCGGATGGCGTCGGAGAGCGCGGTGGTGATATTCAGTATAAGCAGCTGCTGCATGTGCCACGCCATCAAGCGCCTCTTCTGCGGCATGGGCGTCAACCCCACTGTGTACGAGCTGGACGAGGACCCCAGAGGTAAGGACTTGGAGAGGGCGTTGATGAGGCTGCTGGGGACCTCCTCCGCCGTCCCCGTCGTCTTCATCGGTGGCAAACTCGTCGGCGCAATGGACAGAGTCATGGCCTCTCATATCAACGGCACTCTTGTTCCTCTTCTCAAAGAGGCCGGGGCTCTCTGGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glutaredoxin

Protein Analysis

127

Amino Acids

13.79

Weight (kDa)

5.88

Isoelectric Point (pI)

56.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 36 - 99 3.5e-14 Glutaredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 85
AccII CGCG 1 cut(s) 104
AciI CCGC 4 cut(s) 85, 104, 165, 261
AcyI GRCGYC 2 cut(s) 92, 174
AfaI GTAC 1 cut(s) 191
AfiI CCNNNNNNNGG 1 cut(s) 21
AluBI AGCT 2 cut(s) 129, 196
AluI AGCT 2 cut(s) 129, 196
AlwNI CAGNNNCTG 1 cut(s) 132
AoxI GGCC 2 cut(s) 318, 360
ApeKI GCWGC 4 cut(s) 126, 129, 132, 244
AspLEI GCGC 4 cut(s) 78, 104, 156, 302
AspS9I GGNCC 3 cut(s) 64, 205, 252
AsuC2I CCSGG 1 cut(s) 364
AsuHPI GGTGA 1 cut(s) 121
AvaII GGWCC 3 cut(s) 64, 205, 252
BanII GRGCYC 2 cut(s) 29, 370
BbsI GAAGAC 1 cut(s) 268
BbvI GCAGC 4 cut(s) 116, 119, 138, 231
BccI CCATC 2 cut(s) 82, 155
BceAI ACGGC 2 cut(s) 248, 349
BcgI CGANNNNNNTGC 2 cut(s) 277, 311
BcnI CCSGG 1 cut(s) 364
BfoI RGCGCY 1 cut(s) 157
BisI GCNGC 5 cut(s) 127, 130, 133, 166, 245
BlsI GCNGC 5 cut(s) 128, 131, 134, 167, 246
Bme1390I CCNGG 1 cut(s) 364
Bme18I GGWCC 3 cut(s) 64, 205, 252
BmgT120I GGNCC 3 cut(s) 64, 205, 252
BmiI GGNNCC 4 cut(s) 28, 66, 207, 253
BmrFI CCNGG 1 cut(s) 364
BpiI GAAGAC 1 cut(s) 268
BpmI CTGGAG 1 cut(s) 92
BpuMI CCSGG 1 cut(s) 364
BsaHI GRCGYC 2 cut(s) 92, 174
BsaJI CCNNGG 2 cut(s) 51, 363
BsaXI ACNNNNNCTCC 2 cut(s) 218, 248
Bsc4I CCNNNNNNNGG 1 cut(s) 21
Bse3DI GCAATG 1 cut(s) 309
BseDI CCNNGG 2 cut(s) 51, 363
BseGI GGATG 1 cut(s) 93
BseLI CCNNNNNNNGG 1 cut(s) 21
BseMI GCAATG 1 cut(s) 309
BseRI GAGGAG 1 cut(s) 247
BseXI GCAGC 4 cut(s) 116, 119, 138, 231
BseYI CCCAGC 1 cut(s) 247
Bsh1236I CGCG 1 cut(s) 104
BshFI GGCC 2 cut(s) 320, 362
BsiSI CCGG 1 cut(s) 363
BslFI GGGAC 2 cut(s) 251, 265
BslI CCNNNNNNNGG 1 cut(s) 21
BsmFI GGGAC 2 cut(s) 251, 265
BsnI GGCC 2 cut(s) 320, 362
Bsp1286I GDGCHC 2 cut(s) 29, 370
Bsp19I CCATGG 1 cut(s) 51
BspACI CCGC 4 cut(s) 85, 104, 165, 261
BspANI GGCC 2 cut(s) 320, 362
BspFNI CGCG 1 cut(s) 104
BspLI GGNNCC 4 cut(s) 28, 66, 207, 253
BsrBI CCGCTC 1 cut(s) 85
BsrDI GCAATG 1 cut(s) 309
BssECI CCNNGG 2 cut(s) 51, 363
BssNI GRCGYC 2 cut(s) 92, 174
BssT1I CCWWGG 1 cut(s) 51
Bst4CI ACNGT 1 cut(s) 187
Bst6I CTCTTC 2 cut(s) 164, 354
BstACI GRCGYC 2 cut(s) 92, 174
BstAPI GCANNNNNTGC 1 cut(s) 132
BstC8I GCNNGC 1 cut(s) 35
BstDSI CCRYGG 1 cut(s) 51
BstF5I GGATG 1 cut(s) 93
BstFNI CGCG 1 cut(s) 104
BstH2I RGCGCY 1 cut(s) 157
BstHHI GCGC 4 cut(s) 78, 104, 156, 302
BstMWI GCNNNNNNNGC 5 cut(s) 82, 132, 138, 162, 171
BstNSI RCATGY 2 cut(s) 37, 139
BstSCI CCNGG 1 cut(s) 362
BstUI CGCG 1 cut(s) 104
BstV1I GCAGC 4 cut(s) 116, 119, 138, 231
BstV2I GAAGAC 1 cut(s) 268
BsuRI GGCC 2 cut(s) 320, 362
BtgI CCRYGG 1 cut(s) 51
BtsCI GGATG 1 cut(s) 93
BtsIMutI CAGTG 1 cut(s) 183
Cac8I GCNNGC 1 cut(s) 35
CaiI CAGNNNCTG 1 cut(s) 132
CfoI GCGC 4 cut(s) 78, 104, 156, 302
Cfr13I GGNCC 3 cut(s) 64, 205, 252
CseI GACGC 2 cut(s) 81, 163
Csp6I GTAC 1 cut(s) 190
CviAII CATG 6 cut(s) 21, 34, 52, 136, 169, 316
CviJI RGCY 8 cut(s) 27, 129, 196, 244, 320, 362, 368, 376
CviKI_1 RGCY 8 cut(s) 27, 129, 196, 244, 320, 362, 368, 376
CviQI GTAC 1 cut(s) 190
Eam1104I CTCTTC 2 cut(s) 164, 354
EarI CTCTTC 2 cut(s) 164, 354
EciI GGCGGA 1 cut(s) 250
Eco130I CCWWGG 1 cut(s) 51
Eco24I GRGCYC 2 cut(s) 29, 370
Eco47I GGWCC 3 cut(s) 64, 205, 252
EcoO109I RGGNCCY 3 cut(s) 64, 205, 252
EcoT14I CCWWGG 1 cut(s) 51
EcoT38I GRGCYC 2 cut(s) 29, 370
ErhI CCWWGG 1 cut(s) 51
FaeI CATG 6 cut(s) 24, 37, 55, 139, 172, 319
FalI AAGNNNNNCTT 2 cut(s) 143, 175
FaqI GGGAC 2 cut(s) 251, 265
FatI CATG 6 cut(s) 20, 33, 51, 135, 168, 315
Fnu4HI GCNGC 5 cut(s) 127, 130, 133, 166, 245
FokI GGATG 1 cut(s) 100
FriOI GRGCYC 2 cut(s) 29, 370
Fsp4HI GCNGC 5 cut(s) 127, 130, 133, 166, 245
GlaI GCGC 4 cut(s) 77, 103, 155, 301
GluI GCNGC 5 cut(s) 127, 130, 133, 166, 245
GsaI CCCAGC 1 cut(s) 251
GsuI CTGGAG 1 cut(s) 92
HaeII RGCGCY 1 cut(s) 157
HaeIII GGCC 2 cut(s) 320, 362
HapII CCGG 1 cut(s) 363
HgaI GACGC 2 cut(s) 81, 163
HhaI GCGC 4 cut(s) 78, 104, 156, 302
Hin1I GRCGYC 2 cut(s) 92, 174
Hin1II CATG 6 cut(s) 24, 37, 55, 139, 172, 319
Hin6I GCGC 4 cut(s) 76, 102, 154, 300
HinP1I GCGC 4 cut(s) 76, 102, 154, 300
HincII GTYRAC 1 cut(s) 178
HindII GTYRAC 1 cut(s) 178
HinfI GANTC 2 cut(s) 17, 312
HpaII CCGG 1 cut(s) 363
HphI GGTGA 1 cut(s) 121
Hpy166II GTNNAC 2 cut(s) 178, 190
Hpy188I TCNGA 2 cut(s) 97, 380
Hpy188III TCNNGA 1 cut(s) 71
Hpy8I GTNNAC 2 cut(s) 178, 190
Hpy99I CGWCG 3 cut(s) 97, 275, 299
HpyCH4III ACNGT 1 cut(s) 187
HpyCH4V TGCA 3 cut(s) 4, 33, 135
HpyF10VI GCNNNNNNNGC 5 cut(s) 82, 132, 138, 162, 171
Hsp92I GRCGYC 2 cut(s) 92, 174
Hsp92II CATG 6 cut(s) 24, 37, 55, 139, 172, 319
HspAI GCGC 4 cut(s) 76, 102, 154, 300
LmnI GCTCC 2 cut(s) 32, 73
LpnPI CCDG 8 cut(s) 23, 43, 56, 182, 223, 233, 358, 376
Lsp1109I GCAGC 4 cut(s) 116, 119, 138, 231
MbiI CCGCTC 1 cut(s) 85
MboII GAAGA 3 cut(s) 151, 268, 341
MhlI GDGCHC 2 cut(s) 29, 370
MlyI GAGTC 2 cut(s) 26, 321
MmeI TCCRAC 1 cut(s) 75
MspA1I CMGCKG 1 cut(s) 129
MspI CCGG 1 cut(s) 363
MspR9I CCNGG 1 cut(s) 364
MvnI CGCG 1 cut(s) 104
MwoI GCNNNNNNNGC 5 cut(s) 82, 132, 138, 162, 171
NciI CCSGG 1 cut(s) 364
NcoI CCATGG 1 cut(s) 51
NlaIII CATG 6 cut(s) 24, 37, 55, 139, 172, 319
NlaIV GGNNCC 4 cut(s) 28, 66, 207, 253
NspI RCATGY 2 cut(s) 37, 139
PaeI GCATGC 1 cut(s) 37
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PflFI GACNNNGTC 2 cut(s) 16, 311
PkrI GCNGC 5 cut(s) 128, 131, 134, 167, 246
PleI GAGTC 2 cut(s) 25, 320
PpsI GAGTC 2 cut(s) 25, 320
PpuMI RGGWCCY 3 cut(s) 64, 205, 252
Psp5II RGGWCCY 3 cut(s) 64, 205, 252
PspFI CCCAGC 1 cut(s) 247
PspN4I GGNNCC 4 cut(s) 28, 66, 207, 253
PspPI GGNCC 3 cut(s) 64, 205, 252
PspPPI RGGWCCY 3 cut(s) 64, 205, 252
PstNI CAGNNNCTG 1 cut(s) 132
PsyI GACNNNGTC 2 cut(s) 16, 311
PvuII CAGCTG 1 cut(s) 129
RsaI GTAC 1 cut(s) 191
RsaNI GTAC 1 cut(s) 190
SatI GCNGC 5 cut(s) 127, 130, 133, 166, 245
Sau96I GGNCC 3 cut(s) 64, 205, 252
SchI GAGTC 2 cut(s) 26, 321
ScrFI CCNGG 1 cut(s) 364
SduI GDGCHC 2 cut(s) 29, 370
SetI ASST 5 cut(s) 50, 131, 198, 217, 257
SinI GGWCC 3 cut(s) 64, 205, 252
SphI GCATGC 1 cut(s) 37
SsiI CCGC 4 cut(s) 85, 104, 165, 261
StyD4I CCNGG 1 cut(s) 362
StyI CCWWGG 1 cut(s) 51
TaaI ACNGT 1 cut(s) 187
TaqII GACCGA 1 cut(s) 29
TauI GCSGC 1 cut(s) 168
TscAI CASTG 1 cut(s) 190
TseI GCWGC 4 cut(s) 126, 129, 132, 244
TspDTI ATGAA 1 cut(s) 268
TspRI CASTG 1 cut(s) 190
Tth111I GACNNNGTC 2 cut(s) 16, 311
VpaK11BI GGWCC 3 cut(s) 64, 205, 252
XceI RCATGY 2 cut(s) 37, 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.