MD13G1124000.v1.1

glutaredoxin-C1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0004777
Physical Location & Seq
Reverse (-)
9202149 .. 9202532
384 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1124000.v1.1.491

Sequence Viewer

Length: 384 bp
ATGATGCATTACCAGGCAGCTGCTGAGTCATGGGGGTACTACGTGCCGGTGAGGATGAGCATGGGGGACCCACTGGAGCGGGTGGCGAGGCTGGCGTCGAAGAGCGCGGTGGTGATATTCAGCGTGAGCAGCTGCTGCATGTGTCACGCCGTCAAGAGGCTCTTCTGCGGAATGGGGGTGAACCCGACGGTGTACGAGCTGGACCAGGACCCCAGAGGGAAGGAGATCGAGAGGGCTCTCATGAGGTTGCTCGGAACCTCGTCGGCGGTTCCGGTGGTGTTTATTGGTGGCAAGCTTATTGGCGCCATGGATAGAGTCATGGCTTCCCATATCAATGGGTCCCTGGTCCCACTTCTTAAGGAGGCTGGGGCCCTCTGGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glutaredoxin

Protein Analysis

128

Amino Acids

13.82

Weight (kDa)

8.78

Isoelectric Point (pI)

47.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 37 - 100 1.5e-15 Glutaredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 302
AccBSI CCGCTC 1 cut(s) 79
AccII CGCG 1 cut(s) 107
AciI CCGC 4 cut(s) 79, 107, 168, 266
AcyI GRCGYC 2 cut(s) 95, 303
AfaI GTAC 2 cut(s) 38, 194
AfiI CCNNNNNNNGG 1 cut(s) 156
AflII CTTAAG 1 cut(s) 356
AjnI CCWGG 3 cut(s) 12, 204, 342
AluBI AGCT 4 cut(s) 20, 132, 199, 295
AluI AGCT 4 cut(s) 20, 132, 199, 295
AlwNI CAGNNNCTG 2 cut(s) 23, 135
AoxI GGCC 1 cut(s) 369
ApaI GGGCCC 1 cut(s) 373
ApeKI GCWGC 5 cut(s) 17, 20, 129, 132, 135
AspLEI GCGC 2 cut(s) 107, 305
AspS9I GGNCC 7 cut(s) 67, 202, 208, 339, 346, 369, 370
AsuHPI GGTGA 3 cut(s) 61, 124, 190
AvaII GGWCC 5 cut(s) 67, 202, 208, 339, 346
BaeGI GKGCMC 1 cut(s) 373
BanI GGYRCC 1 cut(s) 302
BanII GRGCYC 2 cut(s) 238, 373
BbvI GCAGC 5 cut(s) 7, 29, 119, 122, 141
BceAI ACGGC 1 cut(s) 134
BciT130I CCWGG 3 cut(s) 14, 206, 344
BfoI RGCGCY 1 cut(s) 306
BfrI CTTAAG 1 cut(s) 356
BisI GCNGC 5 cut(s) 18, 21, 130, 133, 136
BlsI GCNGC 5 cut(s) 19, 22, 131, 134, 137
Bme1390I CCNGG 3 cut(s) 14, 206, 344
Bme18I GGWCC 5 cut(s) 67, 202, 208, 339, 346
BmgT120I GGNCC 7 cut(s) 67, 202, 208, 339, 346, 369, 370
BmrFI CCNGG 3 cut(s) 14, 206, 344
BpmI CTGGAG 1 cut(s) 95
BsaAI YACGTR 1 cut(s) 43
BsaHI GRCGYC 2 cut(s) 95, 303
BsaJI CCNNGG 2 cut(s) 306, 342
BsaWI WCCGGW 1 cut(s) 271
Bsc4I CCNNNNNNNGG 1 cut(s) 156
Bse118I RCCGGY 1 cut(s) 46
Bse1I ACTGG 1 cut(s) 78
BseBI CCWGG 3 cut(s) 14, 206, 344
BseDI CCNNGG 2 cut(s) 306, 342
BseGI GGATG 1 cut(s) 60
BseLI CCNNNNNNNGG 1 cut(s) 156
BseMII CTCAG 1 cut(s) 15
BseNI ACTGG 1 cut(s) 78
BseSI GKGCMC 1 cut(s) 373
BseXI GCAGC 5 cut(s) 7, 29, 119, 122, 141
BseYI CCCAGC 1 cut(s) 365
Bsh1236I CGCG 1 cut(s) 107
BshFI GGCC 1 cut(s) 371
BshNI GGYRCC 1 cut(s) 302
BsiSI CCGG 2 cut(s) 47, 272
BslFI GGGAC 3 cut(s) 80, 325, 332
BslI CCNNNNNNNGG 1 cut(s) 156
BsmFI GGGAC 3 cut(s) 80, 325, 332
BsnI GGCC 1 cut(s) 371
Bsp120I GGGCCC 1 cut(s) 369
Bsp1286I GDGCHC 2 cut(s) 238, 373
Bsp143I GATC 1 cut(s) 225
Bsp19I CCATGG 1 cut(s) 306
BspACI CCGC 4 cut(s) 79, 107, 168, 266
BspANI GGCC 1 cut(s) 371
BspCNI CTCAG 1 cut(s) 16
BspFNI CGCG 1 cut(s) 107
BspHI TCATGA 1 cut(s) 240
BspQI GCTCTTC 2 cut(s) 95, 167
BspT107I GGYRCC 1 cut(s) 302
BspTI CTTAAG 1 cut(s) 356
BsrBI CCGCTC 1 cut(s) 79
BsrFI RCCGGY 1 cut(s) 46
BsrI ACTGG 1 cut(s) 78
BssAI RCCGGY 1 cut(s) 46
BssECI CCNNGG 2 cut(s) 306, 342
BssMI GATC 1 cut(s) 225
BssNI GRCGYC 2 cut(s) 95, 303
BssT1I CCWWGG 1 cut(s) 306
Bst2UI CCWGG 3 cut(s) 14, 206, 344
Bst4CI ACNGT 1 cut(s) 190
Bst6I CTCTTC 2 cut(s) 95, 167
BstACI GRCGYC 2 cut(s) 95, 303
BstAFI CTTAAG 1 cut(s) 356
BstAPI GCANNNNNTGC 1 cut(s) 135
BstBAI YACGTR 1 cut(s) 43
BstC8I GCNNGC 2 cut(s) 93, 293
BstDEI CTNAG 1 cut(s) 24
BstDSI CCRYGG 1 cut(s) 306
BstF5I GGATG 1 cut(s) 60
BstFNI CGCG 1 cut(s) 107
BstH2I RGCGCY 1 cut(s) 306
BstHHI GCGC 2 cut(s) 107, 305
BstKTI GATC 1 cut(s) 228
BstMBI GATC 1 cut(s) 225
BstMWI GCNNNNNNNGC 3 cut(s) 92, 129, 135
BstNI CCWGG 3 cut(s) 14, 206, 344
BstNSI RCATGY 1 cut(s) 142
BstSCI CCNGG 3 cut(s) 12, 204, 342
BstSLI GKGCMC 1 cut(s) 373
BstUI CGCG 1 cut(s) 107
BstV1I GCAGC 5 cut(s) 7, 29, 119, 122, 141
BstXI CCANNNNNNTGG 1 cut(s) 335
BsuRI GGCC 1 cut(s) 371
BtgI CCRYGG 1 cut(s) 306
BtsCI GGATG 1 cut(s) 60
BtsIMutI CAGTG 1 cut(s) 71
Cac8I GCNNGC 2 cut(s) 93, 293
CaiI CAGNNNCTG 2 cut(s) 23, 135
CciI TCATGA 1 cut(s) 240
CfoI GCGC 2 cut(s) 107, 305
Cfr10I RCCGGY 1 cut(s) 46
Cfr13I GGNCC 7 cut(s) 67, 202, 208, 339, 346, 369, 370
CseI GACGC 1 cut(s) 84
Csp6I GTAC 2 cut(s) 37, 193
CviAII CATG 6 cut(s) 30, 61, 139, 241, 307, 319
CviQI GTAC 2 cut(s) 37, 193
DdeI CTNAG 1 cut(s) 24
DinI GGCGCC 1 cut(s) 304
DpnI GATC 1 cut(s) 227
DpnII GATC 1 cut(s) 225
Eam1104I CTCTTC 2 cut(s) 95, 167
EarI CTCTTC 2 cut(s) 95, 167
Eco130I CCWWGG 1 cut(s) 306
Eco24I GRGCYC 2 cut(s) 238, 373
Eco47I GGWCC 5 cut(s) 67, 202, 208, 339, 346
EcoO109I RGGNCCY 5 cut(s) 67, 208, 339, 369, 370
EcoRII CCWGG 3 cut(s) 12, 204, 342
EcoT14I CCWWGG 1 cut(s) 306
EcoT22I ATGCAT 1 cut(s) 9
EcoT38I GRGCYC 2 cut(s) 238, 373
EgeI GGCGCC 1 cut(s) 304
EheI GGCGCC 1 cut(s) 304
ErhI CCWWGG 1 cut(s) 306
FaeI CATG 6 cut(s) 33, 64, 142, 244, 310, 322
FaiI YATR 7 cut(s) 31, 62, 140, 242, 308, 320, 330
FalI AAGNNNNNCTT 2 cut(s) 146, 178
FaqI GGGAC 3 cut(s) 80, 325, 332
FatI CATG 6 cut(s) 29, 60, 138, 240, 306, 318
FauI CCCGC 1 cut(s) 72
Fnu4HI GCNGC 5 cut(s) 18, 21, 130, 133, 136
FokI GGATG 1 cut(s) 67
FriOI GRGCYC 2 cut(s) 238, 373
Fsp4HI GCNGC 5 cut(s) 18, 21, 130, 133, 136
GlaI GCGC 2 cut(s) 106, 304
GluI GCNGC 5 cut(s) 18, 21, 130, 133, 136
GsaI CCCAGC 1 cut(s) 369
GsuI CTGGAG 1 cut(s) 95
HaeII RGCGCY 1 cut(s) 306
HaeIII GGCC 1 cut(s) 371
HapII CCGG 2 cut(s) 47, 272
HgaI GACGC 1 cut(s) 84
HhaI GCGC 2 cut(s) 107, 305
Hin1I GRCGYC 2 cut(s) 95, 303
Hin1II CATG 6 cut(s) 33, 64, 142, 244, 310, 322
Hin6I GCGC 2 cut(s) 105, 303
HinP1I GCGC 2 cut(s) 105, 303
HindIII AAGCTT 1 cut(s) 293
HinfI GANTC 2 cut(s) 26, 315
HpaII CCGG 2 cut(s) 47, 272
HphI GGTGA 3 cut(s) 61, 124, 190
Hpy166II GTNNAC 2 cut(s) 181, 193
Hpy188I TCNGA 2 cut(s) 254, 383
Hpy188III TCNNGA 3 cut(s) 154, 229, 241
Hpy8I GTNNAC 2 cut(s) 181, 193
Hpy99I CGWCG 3 cut(s) 100, 190, 265
HpyAV CCTTC 1 cut(s) 214
HpyCH4III ACNGT 1 cut(s) 190
HpyCH4IV ACGT 1 cut(s) 42
HpyCH4V TGCA 2 cut(s) 7, 138
HpyF10VI GCNNNNNNNGC 3 cut(s) 92, 129, 135
HpyF3I CTNAG 1 cut(s) 24
HpySE526I ACGT 1 cut(s) 42
Hsp92I GRCGYC 2 cut(s) 95, 303
Hsp92II CATG 6 cut(s) 33, 64, 142, 244, 310, 322
HspAI GCGC 2 cut(s) 105, 303
KasI GGCGCC 1 cut(s) 302
KflI GGGWCCC 2 cut(s) 67, 339
Kzo9I GATC 1 cut(s) 225
LguI GCTCTTC 2 cut(s) 95, 167
LmnI GCTCC 1 cut(s) 76
Lsp1109I GCAGC 5 cut(s) 7, 29, 119, 122, 141
MaeII ACGT 1 cut(s) 42
MaeIII GTNAC 1 cut(s) 143
MalI GATC 1 cut(s) 227
MbiI CCGCTC 1 cut(s) 79
MboI GATC 1 cut(s) 225
MboII GAAGA 2 cut(s) 112, 154
MhlI GDGCHC 2 cut(s) 238, 373
Mly113I GGCGCC 1 cut(s) 303
MlyI GAGTC 2 cut(s) 35, 324
MnlI CCTC 9 cut(s) 45, 81, 150, 209, 225, 237, 268, 355, 383
Mph1103I ATGCAT 1 cut(s) 9
MseI TTAA 1 cut(s) 357
MslI CAYNNNNRTG 1 cut(s) 333
MspA1I CMGCKG 2 cut(s) 20, 132
MspCI CTTAAG 1 cut(s) 356
MspI CCGG 2 cut(s) 47, 272
MspR9I CCNGG 3 cut(s) 14, 206, 344
MvaI CCWGG 3 cut(s) 14, 206, 344
MvnI CGCG 1 cut(s) 107
MwoI GCNNNNNNNGC 3 cut(s) 92, 129, 135
NarI GGCGCC 1 cut(s) 303
NcoI CCATGG 1 cut(s) 306
NdeII GATC 1 cut(s) 225
NlaIII CATG 6 cut(s) 33, 64, 142, 244, 310, 322
NmuCI GTSAC 1 cut(s) 143
NsiI ATGCAT 1 cut(s) 9
NspI RCATGY 1 cut(s) 142
PagI TCATGA 1 cut(s) 240
PciSI GCTCTTC 2 cut(s) 95, 167
PkrI GCNGC 5 cut(s) 19, 22, 131, 134, 137
PleI GAGTC 2 cut(s) 34, 323
PluTI GGCGCC 1 cut(s) 306
PpsI GAGTC 2 cut(s) 34, 323
Ppu21I YACGTR 1 cut(s) 43
PpuMI RGGWCCY 3 cut(s) 67, 208, 339
Psp5II RGGWCCY 3 cut(s) 67, 208, 339
Psp6I CCWGG 3 cut(s) 12, 204, 342
PspFI CCCAGC 1 cut(s) 365
PspGI CCWGG 3 cut(s) 12, 204, 342
PspOMI GGGCCC 1 cut(s) 369
PspPI GGNCC 7 cut(s) 67, 202, 208, 339, 346, 369, 370
PspPPI RGGWCCY 3 cut(s) 67, 208, 339
PstNI CAGNNNCTG 2 cut(s) 23, 135
PvuII CAGCTG 2 cut(s) 20, 132
RsaI GTAC 2 cut(s) 38, 194
RsaNI GTAC 2 cut(s) 37, 193
RseI CAYNNNNRTG 1 cut(s) 333
SapI GCTCTTC 2 cut(s) 95, 167
SaqAI TTAA 1 cut(s) 357
SatI GCNGC 5 cut(s) 18, 21, 130, 133, 136
Sau3AI GATC 1 cut(s) 225
Sau96I GGNCC 7 cut(s) 67, 202, 208, 339, 346, 369, 370
SchI GAGTC 2 cut(s) 35, 324
ScrFI CCNGG 3 cut(s) 14, 206, 344
SduI GDGCHC 2 cut(s) 238, 373
SetI ASST 7 cut(s) 22, 45, 134, 201, 248, 260, 297
SfoI GGCGCC 1 cut(s) 304
SinI GGWCC 5 cut(s) 67, 202, 208, 339, 346
SmiMI CAYNNNNRTG 1 cut(s) 333
SmlI CTYRAG 1 cut(s) 356
SmoI CTYRAG 1 cut(s) 356
SsiI CCGC 4 cut(s) 79, 107, 168, 266
SspDI GGCGCC 1 cut(s) 302
StyD4I CCNGG 3 cut(s) 12, 204, 342
StyI CCWWGG 1 cut(s) 306
TaaI ACNGT 1 cut(s) 190
TaiI ACGT 1 cut(s) 45
TaqI TCGA 2 cut(s) 98, 228
Tru1I TTAA 1 cut(s) 357
Tru9I TTAA 1 cut(s) 357
TscAI CASTG 1 cut(s) 78
TseFI GTSAC 1 cut(s) 143
TseI GCWGC 5 cut(s) 17, 20, 129, 132, 135
Tsp45I GTSAC 1 cut(s) 143
TspRI CASTG 1 cut(s) 78
Vha464I CTTAAG 1 cut(s) 356
VpaK11BI GGWCC 5 cut(s) 67, 202, 208, 339, 346
XceI RCATGY 1 cut(s) 142
Zsp2I ATGCAT 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.