MD09G1009900.v1.1

Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0003993
Physical Location & Seq
Reverse (-)
676891 .. 677637
747 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1009900.v1.1.491

Sequence Viewer

Length: 660 bp
ATGACTGCAGTGGAAGCACAATGCCTTGGTGTCGTCTCTTGTGCTGACATCTTGGCTATGGCTGCCCGGGATTGTGTATTCTTTGCAGGAGGCCCTTCGTTCCCAGTAGAATTGGGACGCCATGATGGACTCATCTCGAAAGCATCACGGGTTGCTGGGAACTTGCCAGAACCGTTCCTTGATCTGTTGCAACTCAATACCATGTTTGCCAAACATAATCTTTCCCTAACTGATGTCATTGCGTTGTCCGGAGCTCATACTCTAGGCGTTTCACACTGCAATCGCTTCTCAGACCGCCTCTTCAACTTCTCATCGTCCTCTCCTGTCGACCCTTCTCTGAATCCTGACTATGCCAAGCGGTTGATGGCAACCTGCTCCAAAGAGGCAGACCCCGGCGCAATCAGCGACTTGGACCCTGACACCCCATTCACATTTGACAATGTTTATTACAAAAACTTGGTTGGCGGGAAAGGGTTGTTGAGCTCAGATCAGGTTCTTTTCTCCGACTCTGCATCACAGCCCACTGTCCTTGATTTTGCTAAGAACCCAGGTAACTTCGATGGAGCCTTCGTCGCTGCCATGAGGAAGCTTGGAAGAGTCGGTGTTAAGACTGGTATTCAAGGGGAGGTCAGGAGAGACTGCACCACCTTCAATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
peroxidase

Protein Analysis

220

Amino Acids

23.35

Weight (kDa)

5.5

Isoelectric Point (pI)

32.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 2 - 182 1.1e-56 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 380
AccI GTMKAC 1 cut(s) 327
AccIII TCCGGA 1 cut(s) 248
AciI CCGC 3 cut(s) 295, 358, 465
AcyI GRCGYC 1 cut(s) 118
AgsI TTSAA 3 cut(s) 304, 620, 652
AjnI CCWGG 1 cut(s) 547
AluBI AGCT 3 cut(s) 254, 483, 589
AluI AGCT 3 cut(s) 254, 483, 589
Alw21I GWGCWC 2 cut(s) 256, 485
Alw26I GTCTC 2 cut(s) 40, 630
Ama87I CYCGRG 1 cut(s) 66
Aor13HI TCCGGA 1 cut(s) 248
AoxI GGCC 1 cut(s) 91
ApeKI GCWGC 2 cut(s) 62, 575
AspLEI GCGC 1 cut(s) 398
AspS9I GGNCC 2 cut(s) 92, 412
AsuC2I CCSGG 3 cut(s) 67, 68, 393
AvaI CYCGRG 1 cut(s) 66
AvaII GGWCC 1 cut(s) 412
BanII GRGCYC 2 cut(s) 256, 485
Bbv12I GWGCWC 2 cut(s) 256, 485
BbvI GCAGC 2 cut(s) 49, 562
BccI CCATC 3 cut(s) 119, 358, 554
BciT130I CCWGG 1 cut(s) 549
BcnI CCSGG 3 cut(s) 67, 68, 393
BcoDI GTCTC 2 cut(s) 40, 630
BfaI CTAG 1 cut(s) 263
BfmI CTRYAG 1 cut(s) 6
BfuAI ACCTGC 1 cut(s) 380
BisI GCNGC 2 cut(s) 63, 576
BlsI GCNGC 2 cut(s) 64, 577
Bme1390I CCNGG 4 cut(s) 67, 68, 393, 549
Bme18I GGWCC 1 cut(s) 412
BmeT110I CYCGRG 1 cut(s) 66
BmgT120I GGNCC 2 cut(s) 92, 412
BmiI GGNNCC 2 cut(s) 414, 565
BmrFI CCNGG 4 cut(s) 67, 68, 393, 549
BmrI ACTGGG 1 cut(s) 98
BmsI GCATC 2 cut(s) 152, 521
BmuI ACTGGG 1 cut(s) 98
BpuMI CCSGG 3 cut(s) 67, 68, 393
BsaHI GRCGYC 1 cut(s) 118
BsaJI CCNNGG 4 cut(s) 25, 66, 391, 547
BsaWI WCCGGW 1 cut(s) 248
BsaXI ACNNNNNCTCC 2 cut(s) 555, 585
Bse1I ACTGG 2 cut(s) 104, 616
Bse3DI GCAATG 1 cut(s) 237
BseAI TCCGGA 1 cut(s) 248
BseBI CCWGG 1 cut(s) 549
BseDI CCNNGG 4 cut(s) 25, 66, 391, 547
BseMI GCAATG 1 cut(s) 237
BseMII CTCAG 2 cut(s) 303, 498
BseNI ACTGG 2 cut(s) 104, 616
BseXI GCAGC 2 cut(s) 49, 562
BseYI CCCAGC 1 cut(s) 155
BsgI GTGCAG 1 cut(s) 625
BshFI GGCC 1 cut(s) 93
BsiHKAI GWGCWC 2 cut(s) 256, 485
BsiHKCI CYCGRG 1 cut(s) 66
BsiSI CCGG 3 cut(s) 67, 249, 393
BslFI GGGAC 1 cut(s) 129
BsmAI GTCTC 2 cut(s) 40, 630
BsmBI CGTCTC 1 cut(s) 40
BsmFI GGGAC 1 cut(s) 129
BsnI GGCC 1 cut(s) 93
BsoBI CYCGRG 1 cut(s) 66
Bsp1286I GDGCHC 2 cut(s) 256, 485
Bsp13I TCCGGA 1 cut(s) 248
Bsp143I GATC 2 cut(s) 181, 487
BspACI CCGC 3 cut(s) 295, 358, 465
BspANI GGCC 1 cut(s) 93
BspCNI CTCAG 2 cut(s) 302, 497
BspEI TCCGGA 1 cut(s) 248
BspHI TCATGA 1 cut(s) 656
BspLI GGNNCC 2 cut(s) 414, 565
BspMAI CTGCAG 1 cut(s) 10
BspMI ACCTGC 1 cut(s) 380
BsrDI GCAATG 1 cut(s) 237
BsrI ACTGG 2 cut(s) 104, 616
BssECI CCNNGG 4 cut(s) 25, 66, 391, 547
BssMI GATC 2 cut(s) 181, 487
BssNI GRCGYC 1 cut(s) 118
BssT1I CCWWGG 1 cut(s) 25
Bst2UI CCWGG 1 cut(s) 549
Bst4CI ACNGT 2 cut(s) 174, 526
Bst6I CTCTTC 2 cut(s) 305, 589
BstACI GRCGYC 1 cut(s) 118
BstDEI CTNAG 3 cut(s) 289, 484, 540
BstHHI GCGC 1 cut(s) 398
BstKTI GATC 2 cut(s) 184, 490
BstMAI GTCTC 2 cut(s) 40, 630
BstMBI GATC 2 cut(s) 181, 487
BstMWI GCNNNNNNNGC 4 cut(s) 14, 62, 402, 572
BstNI CCWGG 1 cut(s) 549
BstSCI CCNGG 4 cut(s) 65, 66, 391, 547
BstSFI CTRYAG 1 cut(s) 6
BstV1I GCAGC 2 cut(s) 49, 562
BsuRI GGCC 1 cut(s) 93
BtsI GCAGTG 2 cut(s) 15, 274
BtsIMutI CAGTG 3 cut(s) 15, 274, 522
BveI ACCTGC 1 cut(s) 380
CciI TCATGA 1 cut(s) 656
CfoI GCGC 1 cut(s) 398
Cfr13I GGNCC 2 cut(s) 92, 412
Cfr9I CCCGGG 1 cut(s) 66
CseI GACGC 1 cut(s) 126
CspCI CAANNNNNGTGG 2 cut(s) 511, 546
CviAII CATG 4 cut(s) 122, 202, 580, 657
CviJI RGCY 8 cut(s) 56, 62, 93, 254, 483, 520, 566, 589
CviKI_1 RGCY 8 cut(s) 56, 62, 93, 254, 483, 520, 566, 589
DdeI CTNAG 3 cut(s) 289, 484, 540
DpnI GATC 2 cut(s) 183, 489
DpnII GATC 2 cut(s) 181, 487
Eam1104I CTCTTC 2 cut(s) 305, 589
EarI CTCTTC 2 cut(s) 305, 589
Ecl136II GAGCTC 2 cut(s) 254, 483
Eco130I CCWWGG 1 cut(s) 25
Eco24I GRGCYC 2 cut(s) 256, 485
Eco47I GGWCC 1 cut(s) 412
Eco53kI GAGCTC 2 cut(s) 254, 483
Eco88I CYCGRG 1 cut(s) 66
EcoICRI GAGCTC 2 cut(s) 254, 483
EcoO109I RGGNCCY 1 cut(s) 92
EcoRII CCWGG 1 cut(s) 547
EcoT14I CCWWGG 1 cut(s) 25
EcoT38I GRGCYC 2 cut(s) 256, 485
ErhI CCWWGG 1 cut(s) 25
Esp3I CGTCTC 1 cut(s) 40
FaeI CATG 4 cut(s) 125, 205, 583, 660
FaiI YATR 8 cut(s) 59, 123, 203, 216, 258, 351, 581, 658
FaqI GGGAC 1 cut(s) 129
FatI CATG 4 cut(s) 121, 201, 579, 656
FauI CCCGC 1 cut(s) 458
FblI GTMKAC 1 cut(s) 327
Fnu4HI GCNGC 2 cut(s) 63, 576
FriOI GRGCYC 2 cut(s) 256, 485
Fsp4HI GCNGC 2 cut(s) 63, 576
FspBI CTAG 1 cut(s) 263
GlaI GCGC 1 cut(s) 397
GluI GCNGC 2 cut(s) 63, 576
GsaI CCCAGC 1 cut(s) 159
HaeIII GGCC 1 cut(s) 93
HapII CCGG 3 cut(s) 67, 249, 393
HgaI GACGC 1 cut(s) 126
HhaI GCGC 1 cut(s) 398
Hin1I GRCGYC 1 cut(s) 118
Hin1II CATG 4 cut(s) 125, 205, 583, 660
Hin6I GCGC 1 cut(s) 396
HinP1I GCGC 1 cut(s) 396
HincII GTYRAC 1 cut(s) 328
HindII GTYRAC 1 cut(s) 328
HindIII AAGCTT 1 cut(s) 587
HinfI GANTC 4 cut(s) 129, 340, 506, 597
HpaII CCGG 3 cut(s) 67, 249, 393
Hpy166II GTNNAC 1 cut(s) 328
Hpy188I TCNGA 4 cut(s) 292, 339, 487, 505
Hpy188III TCNNGA 5 cut(s) 136, 249, 344, 631, 657
Hpy8I GTNNAC 1 cut(s) 328
Hpy99I CGWCG 1 cut(s) 575
HpyAV CCTTC 4 cut(s) 105, 342, 577, 658
HpyCH4III ACNGT 2 cut(s) 174, 526
HpyCH4V TGCA 6 cut(s) 8, 86, 190, 279, 512, 642
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 62, 402, 572
HpyF3I CTNAG 3 cut(s) 289, 484, 540
Hsp92I GRCGYC 1 cut(s) 118
Hsp92II CATG 4 cut(s) 125, 205, 583, 660
HspAI GCGC 1 cut(s) 396
Kpn2I TCCGGA 1 cut(s) 248
Kzo9I GATC 2 cut(s) 181, 487
LmnI GCTCC 3 cut(s) 251, 380, 563
Lsp1109I GCAGC 2 cut(s) 49, 562
LweI GCATC 2 cut(s) 152, 521
MaeI CTAG 1 cut(s) 263
MaeIII GTNAC 1 cut(s) 551
MalI GATC 2 cut(s) 183, 489
MboI GATC 2 cut(s) 181, 487
MboII GAAGA 2 cut(s) 292, 606
MhlI GDGCHC 2 cut(s) 256, 485
MluCI AATT 2 cut(s) 110, 652
MlyI GAGTC 3 cut(s) 123, 500, 606
MmeI TCCRAC 1 cut(s) 528
MnlI CCTC 6 cut(s) 83, 308, 328, 376, 576, 619
MroI TCCGGA 1 cut(s) 248
MseI TTAA 1 cut(s) 606
MspI CCGG 3 cut(s) 67, 249, 393
MspR9I CCNGG 4 cut(s) 67, 68, 393, 549
MvaI CCWGG 1 cut(s) 549
MwoI GCNNNNNNNGC 4 cut(s) 14, 62, 402, 572
NciI CCSGG 3 cut(s) 67, 68, 393
NdeII GATC 2 cut(s) 181, 487
NlaIII CATG 4 cut(s) 125, 205, 583, 660
NlaIV GGNNCC 2 cut(s) 414, 565
PagI TCATGA 1 cut(s) 656
PfeI GAWTC 1 cut(s) 340
PkrI GCNGC 2 cut(s) 64, 577
PleI GAGTC 3 cut(s) 123, 500, 605
PpsI GAGTC 3 cut(s) 123, 500, 605
Psp124BI GAGCTC 2 cut(s) 256, 485
Psp6I CCWGG 1 cut(s) 547
PspFI CCCAGC 1 cut(s) 155
PspGI CCWGG 1 cut(s) 547
PspN4I GGNNCC 2 cut(s) 414, 565
PspPI GGNCC 2 cut(s) 92, 412
PstI CTGCAG 1 cut(s) 10
SacI GAGCTC 2 cut(s) 256, 485
SalI GTCGAC 1 cut(s) 326
SaqAI TTAA 1 cut(s) 606
SatI GCNGC 2 cut(s) 63, 576
Sau3AI GATC 2 cut(s) 181, 487
Sau96I GGNCC 2 cut(s) 92, 412
SchI GAGTC 3 cut(s) 123, 500, 606
ScrFI CCNGG 4 cut(s) 67, 68, 393, 549
SduI GDGCHC 2 cut(s) 256, 485
SetI ASST 8 cut(s) 256, 374, 485, 495, 553, 591, 630, 650
SfaNI GCATC 2 cut(s) 152, 521
SfcI CTRYAG 1 cut(s) 6
SinI GGWCC 1 cut(s) 412
SmaI CCCGGG 1 cut(s) 68
Sse9I AATT 2 cut(s) 110, 652
SsiI CCGC 3 cut(s) 295, 358, 465
SspMI CTAG 1 cut(s) 263
SstI GAGCTC 2 cut(s) 256, 485
StyD4I CCNGG 4 cut(s) 65, 66, 391, 547
StyI CCWWGG 1 cut(s) 25
TaaI ACNGT 2 cut(s) 174, 526
TaqI TCGA 3 cut(s) 137, 327, 558
TasI AATT 2 cut(s) 110, 652
TfiI GAWTC 1 cut(s) 340
Tru1I TTAA 1 cut(s) 606
Tru9I TTAA 1 cut(s) 606
TscAI CASTG 3 cut(s) 15, 281, 529
TseI GCWGC 2 cut(s) 62, 575
TspDTI ATGAA 1 cut(s) 645
TspMI CCCGGG 1 cut(s) 66
TspRI CASTG 3 cut(s) 15, 281, 529
VpaK11BI GGWCC 1 cut(s) 412
XcmI CCANNNNNNNNNTGG 1 cut(s) 361
XmaI CCCGGG 1 cut(s) 66
XmiI GTMKAC 1 cut(s) 327
XspI CTAG 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.