pycom111g00790

Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0003993
Physical Location & Seq
Reverse (-)
541382 .. 541840
459 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g00790.1

Sequence Viewer

Length: 459 bp
ATGTTTGCCAAACACAATGTTTCCCTAACTGATGTCATTGCATTGTCCGGAGCTCATACTCTAGGCGTTTCACACTGCAATCGCTTCTCAGACCGCCTCTACAACTTCACATCGTCCTCTCCTGTCGACCCTTCTCTGAATCCTGATTATGCCAAGCGGTTGATGGTAACCTGCTCCAAAGAGGCAGACCCCGACACAATCAGCGACTTGGACCCTGGTACCCCATTCACATTTGACAATGTTTATTGCAAAAACTTGGTTGGCGGGAAAGGGTTGTTGAGCTCAGATCAGGTTCTTTTCTTCGACTCTGCATCACAGCCCACTGTCCTTGATTTTGCTAAGAACCCAAGTAACTTCAATGGAGCCTTCGTCGCTGCCATGAGGAAGCTTGGAAGAGTCGGTGTTAAGACTGGTAATCAAGGGGAGATCAGGAGAGACTGCACCACCTTCAATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
peroxidase

Protein Analysis

153

Amino Acids

16.48

Weight (kDa)

6.81

Isoelectric Point (pI)

24.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 1 - 115 7.4e-29 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 179
Acc65I GGTACC 1 cut(s) 218
AccB1I GGYRCC 1 cut(s) 218
AccI GTMKAC 1 cut(s) 126
AccIII TCCGGA 1 cut(s) 47
AciI CCGC 3 cut(s) 94, 157, 264
AfaI GTAC 1 cut(s) 220
AgsI TTSAA 2 cut(s) 358, 451
AjnI CCWGG 1 cut(s) 214
AluBI AGCT 3 cut(s) 53, 282, 388
AluI AGCT 3 cut(s) 53, 282, 388
Alw21I GWGCWC 2 cut(s) 55, 284
Alw26I GTCTC 1 cut(s) 429
Aor13HI TCCGGA 1 cut(s) 47
ApeKI GCWGC 1 cut(s) 374
Asp718I GGTACC 1 cut(s) 218
AspS9I GGNCC 1 cut(s) 211
AvaII GGWCC 1 cut(s) 211
BaeI ACNNNNGTAYC 2 cut(s) 202, 235
BanI GGYRCC 1 cut(s) 218
BanII GRGCYC 2 cut(s) 55, 284
Bbv12I GWGCWC 2 cut(s) 55, 284
BbvI GCAGC 1 cut(s) 361
BccI CCATC 1 cut(s) 157
BciT130I CCWGG 1 cut(s) 216
BcoDI GTCTC 1 cut(s) 429
BfaI CTAG 1 cut(s) 62
BfuAI ACCTGC 1 cut(s) 179
BisI GCNGC 1 cut(s) 375
BlsI GCNGC 1 cut(s) 376
Bme1390I CCNGG 1 cut(s) 216
Bme18I GGWCC 1 cut(s) 211
BmgT120I GGNCC 1 cut(s) 211
BmiI GGNNCC 3 cut(s) 213, 220, 364
BmrFI CCNGG 1 cut(s) 216
BmsI GCATC 1 cut(s) 320
BsaJI CCNNGG 1 cut(s) 214
BsaWI WCCGGW 1 cut(s) 47
BsaXI ACNNNNNCTCC 2 cut(s) 354, 384
Bse1I ACTGG 1 cut(s) 415
Bse3DI GCAATG 1 cut(s) 36
BseAI TCCGGA 1 cut(s) 47
BseBI CCWGG 1 cut(s) 216
BseDI CCNNGG 1 cut(s) 214
BseMI GCAATG 1 cut(s) 36
BseMII CTCAG 2 cut(s) 102, 297
BseNI ACTGG 1 cut(s) 415
BseXI GCAGC 1 cut(s) 361
BsgI GTGCAG 1 cut(s) 424
BshNI GGYRCC 1 cut(s) 218
BsiHKAI GWGCWC 2 cut(s) 55, 284
BsiSI CCGG 1 cut(s) 48
BsmAI GTCTC 1 cut(s) 429
Bsp1286I GDGCHC 2 cut(s) 55, 284
Bsp13I TCCGGA 1 cut(s) 47
Bsp143I GATC 2 cut(s) 286, 426
BspACI CCGC 3 cut(s) 94, 157, 264
BspCNI CTCAG 2 cut(s) 101, 296
BspEI TCCGGA 1 cut(s) 47
BspHI TCATGA 1 cut(s) 455
BspLI GGNNCC 3 cut(s) 213, 220, 364
BspMI ACCTGC 1 cut(s) 179
BspT107I GGYRCC 1 cut(s) 218
BsrDI GCAATG 1 cut(s) 36
BsrI ACTGG 1 cut(s) 415
BssECI CCNNGG 1 cut(s) 214
BssMI GATC 2 cut(s) 286, 426
Bst2UI CCWGG 1 cut(s) 216
Bst4CI ACNGT 1 cut(s) 325
Bst6I CTCTTC 1 cut(s) 388
BstDEI CTNAG 3 cut(s) 88, 283, 339
BstEII GGTNACC 1 cut(s) 166
BstKTI GATC 2 cut(s) 289, 429
BstMAI GTCTC 1 cut(s) 429
BstMBI GATC 2 cut(s) 286, 426
BstMWI GCNNNNNNNGC 1 cut(s) 371
BstNI CCWGG 1 cut(s) 216
BstPI GGTNACC 1 cut(s) 166
BstSCI CCNGG 1 cut(s) 214
BstV1I GCAGC 1 cut(s) 361
BtsI GCAGTG 1 cut(s) 73
BtsIMutI CAGTG 2 cut(s) 73, 321
BveI ACCTGC 1 cut(s) 179
CciI TCATGA 1 cut(s) 455
Cfr13I GGNCC 1 cut(s) 211
Csp6I GTAC 1 cut(s) 219
CspCI CAANNNNNGTGG 2 cut(s) 310, 345
CviAII CATG 2 cut(s) 379, 456
CviJI RGCY 5 cut(s) 53, 282, 319, 365, 388
CviKI_1 RGCY 5 cut(s) 53, 282, 319, 365, 388
CviQI GTAC 1 cut(s) 219
DdeI CTNAG 3 cut(s) 88, 283, 339
DpnI GATC 2 cut(s) 288, 428
DpnII GATC 2 cut(s) 286, 426
Eam1104I CTCTTC 1 cut(s) 388
EarI CTCTTC 1 cut(s) 388
Ecl136II GAGCTC 2 cut(s) 53, 282
Eco24I GRGCYC 2 cut(s) 55, 284
Eco47I GGWCC 1 cut(s) 211
Eco53kI GAGCTC 2 cut(s) 53, 282
Eco91I GGTNACC 1 cut(s) 166
EcoICRI GAGCTC 2 cut(s) 53, 282
EcoO65I GGTNACC 1 cut(s) 166
EcoRII CCWGG 1 cut(s) 214
EcoT38I GRGCYC 2 cut(s) 55, 284
FaeI CATG 2 cut(s) 382, 459
FaiI YATR 4 cut(s) 57, 150, 380, 457
FatI CATG 2 cut(s) 378, 455
FauI CCCGC 1 cut(s) 257
FblI GTMKAC 1 cut(s) 126
Fnu4HI GCNGC 1 cut(s) 375
FriOI GRGCYC 2 cut(s) 55, 284
Fsp4HI GCNGC 1 cut(s) 375
FspBI CTAG 1 cut(s) 62
GluI GCNGC 1 cut(s) 375
HapII CCGG 1 cut(s) 48
Hin1II CATG 2 cut(s) 382, 459
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HindIII AAGCTT 1 cut(s) 386
HinfI GANTC 3 cut(s) 139, 305, 396
HpaII CCGG 1 cut(s) 48
Hpy166II GTNNAC 1 cut(s) 127
Hpy188I TCNGA 3 cut(s) 91, 138, 286
Hpy188III TCNNGA 4 cut(s) 48, 143, 430, 456
Hpy8I GTNNAC 1 cut(s) 127
Hpy99I CGWCG 1 cut(s) 374
HpyAV CCTTC 3 cut(s) 141, 376, 457
HpyCH4III ACNGT 1 cut(s) 325
HpyCH4V TGCA 5 cut(s) 41, 78, 249, 311, 441
HpyF10VI GCNNNNNNNGC 1 cut(s) 371
HpyF3I CTNAG 3 cut(s) 88, 283, 339
Hsp92II CATG 2 cut(s) 382, 459
Kpn2I TCCGGA 1 cut(s) 47
KpnI GGTACC 1 cut(s) 222
Kzo9I GATC 2 cut(s) 286, 426
LmnI GCTCC 3 cut(s) 50, 179, 362
LpnPI CCDG 9 cut(s) 61, 135, 156, 184, 201, 228, 275, 396, 415
Lsp1109I GCAGC 1 cut(s) 361
LweI GCATC 1 cut(s) 320
MaeI CTAG 1 cut(s) 62
MaeIII GTNAC 2 cut(s) 166, 350
MalI GATC 2 cut(s) 288, 428
MboI GATC 2 cut(s) 286, 426
MboII GAAGA 2 cut(s) 292, 405
MhlI GDGCHC 2 cut(s) 55, 284
MluCI AATT 1 cut(s) 451
MlyI GAGTC 2 cut(s) 299, 405
MnlI CCTC 4 cut(s) 107, 127, 175, 375
MroI TCCGGA 1 cut(s) 47
MseI TTAA 1 cut(s) 405
MspI CCGG 1 cut(s) 48
MspR9I CCNGG 1 cut(s) 216
MvaI CCWGG 1 cut(s) 216
MwoI GCNNNNNNNGC 1 cut(s) 371
NdeII GATC 2 cut(s) 286, 426
NlaIII CATG 2 cut(s) 382, 459
NlaIV GGNNCC 3 cut(s) 213, 220, 364
PagI TCATGA 1 cut(s) 455
PfeI GAWTC 1 cut(s) 139
PkrI GCNGC 1 cut(s) 376
PleI GAGTC 2 cut(s) 299, 404
PpsI GAGTC 2 cut(s) 299, 404
Psp124BI GAGCTC 2 cut(s) 55, 284
Psp6I CCWGG 1 cut(s) 214
PspEI GGTNACC 1 cut(s) 166
PspGI CCWGG 1 cut(s) 214
PspN4I GGNNCC 3 cut(s) 213, 220, 364
PspPI GGNCC 1 cut(s) 211
RsaI GTAC 1 cut(s) 220
RsaNI GTAC 1 cut(s) 219
SacI GAGCTC 2 cut(s) 55, 284
SalI GTCGAC 1 cut(s) 125
SaqAI TTAA 1 cut(s) 405
SatI GCNGC 1 cut(s) 375
Sau3AI GATC 2 cut(s) 286, 426
Sau96I GGNCC 1 cut(s) 211
SchI GAGTC 2 cut(s) 299, 405
ScrFI CCNGG 1 cut(s) 216
SduI GDGCHC 2 cut(s) 55, 284
SetI ASST 6 cut(s) 55, 173, 284, 294, 390, 449
SfaNI GCATC 1 cut(s) 320
SinI GGWCC 1 cut(s) 211
Sse9I AATT 1 cut(s) 451
SsiI CCGC 3 cut(s) 94, 157, 264
SspMI CTAG 1 cut(s) 62
SstI GAGCTC 2 cut(s) 55, 284
StyD4I CCNGG 1 cut(s) 214
TaaI ACNGT 1 cut(s) 325
TaqI TCGA 2 cut(s) 126, 303
TasI AATT 1 cut(s) 451
TfiI GAWTC 1 cut(s) 139
Tru1I TTAA 1 cut(s) 405
Tru9I TTAA 1 cut(s) 405
TscAI CASTG 2 cut(s) 80, 328
TseI GCWGC 1 cut(s) 374
TspDTI ATGAA 1 cut(s) 444
TspRI CASTG 2 cut(s) 80, 328
VpaK11BI GGWCC 1 cut(s) 211
XcmI CCANNNNNNNNNTGG 1 cut(s) 160
XmiI GTMKAC 1 cut(s) 126
XspI CTAG 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.