MD09G1021700.v1.1

Surfeit locus protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0007923
Physical Location & Seq
Reverse (-)
1316121 .. 1318007
1887 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1021700.v1.1.491

Sequence Viewer

Length: 789 bp
ATGGCGACGACTGAGAAGGCGAAGGAGGAAAAGGAAGGGAGCAACCTGCTGGGCTCGCCGACCTTCAAGGAGCTCGACAACGGCCGCTTCCAGTGCGTCGAGACAGGCCACGAATTGCTGGCCAAAGACATAGAAATTTACTCTCGGAGCAAACGGTGCCGTTTGGGTCTCATCGAATATTCCCTTGCCCATAAGAAAGCTCCTCTCAACATGTTCAAGCAGGATCCTCTTTCCCGTTCAAAGTTGGTGTGTAAGCTAACAGGGGATACTATCAATAAGTCAGAGGAGCACATTTGGAAGCATATAAATGGGAAACGGTTCCTCAACAAGCTCGAAGAGAAGGAAGAAGAGAAGCTAAAAGCCAAGGGAGTGGTAGAAAATATGGATGATCAGAACCCGGAAATAGTACCTGACCAAGACGGAGACGGAGAAAGAAAGAAGAAGAAGAAAAAGAATAAGAAGAAGAAGAATAAGAAGAAGGATGACAGAGTGGATGAGATTATGTCTGGAAAAAGTAAGTCTTCTGATGAGGAAGGTGATACTGAAGAAGCTGACTTTTGGATGCCACCAGTGGGAGAACGTTGGGACTTTGACGAGGGAGGTGACAGGTGGGGTTCCGGTTCAGAGTCAGAGAACGAGGATGAAGAGATCGATGGAGCTGATGGTGCATTTGAAGATGGCGACGAGGAGTCAGAAGAGCTCTCCAAGAGGACGAAAAGGATGTCCATAGAAATTGGACCAAGCAGCTTTGCTTCAAGGAAGAAGAAGAGTAAAAAGAGCACGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SURF2

Protein Analysis

263

Amino Acids

29.84

Weight (kDa)

5.58

Isoelectric Point (pI)

66.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SURF2 PF05477 10 - 258 1.5e-59 Surfeit locus protein 2 (SURF2)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 54
AccB1I GGYRCC 1 cut(s) 156
AciI CCGC 1 cut(s) 85
AclI AACGTT 1 cut(s) 580
AclWI GGATC 2 cut(s) 218, 231
AcoI YGGCCR 2 cut(s) 82, 120
AcsI RAATTY 1 cut(s) 135
AcuI CTGAAG 1 cut(s) 564
AfaI GTAC 1 cut(s) 408
AfiI CCNNNNNNNGG 1 cut(s) 572
AflIII ACRYGT 1 cut(s) 210
AgsI TTSAA 5 cut(s) 67, 217, 240, 674, 756
AhdI GACNNNNNGTC 1 cut(s) 688
AluBI AGCT 9 cut(s) 73, 200, 256, 331, 355, 551, 659, 700, 747
AluI AGCT 9 cut(s) 73, 200, 256, 331, 355, 551, 659, 700, 747
Alw21I GWGCWC 4 cut(s) 75, 291, 702, 782
Alw26I GTCTC 3 cut(s) 95, 173, 417
AlwI GGATC 2 cut(s) 218, 231
AoxI GGCC 3 cut(s) 82, 106, 120
ApeKI GCWGC 1 cut(s) 744
ApoI RAATTY 1 cut(s) 135
ArsI GACNNNNNNTTYG 2 cut(s) 707, 739
Asp700I GAANNNNTTC 1 cut(s) 317
AspS9I GGNCC 1 cut(s) 737
AsuC2I CCSGG 1 cut(s) 398
AsuHPI GGTGA 2 cut(s) 548, 614
AvaII GGWCC 1 cut(s) 737
BalI TGGCCA 1 cut(s) 122
BamHI GGATCC 1 cut(s) 223
BanI GGYRCC 1 cut(s) 156
BanII GRGCYC 3 cut(s) 56, 75, 702
BbsI GAAGAC 1 cut(s) 513
Bbv12I GWGCWC 4 cut(s) 75, 291, 702, 782
BbvI GCAGC 1 cut(s) 756
BccI CCATC 3 cut(s) 647, 656, 671
BceAI ACGGC 2 cut(s) 97, 144
BciVI GTATCC 1 cut(s) 259
BclI TGATCA 1 cut(s) 388
BcnI CCSGG 1 cut(s) 398
BcoDI GTCTC 3 cut(s) 95, 173, 417
BfuAI ACCTGC 1 cut(s) 54
BfuI GTATCC 1 cut(s) 259
BisI GCNGC 2 cut(s) 85, 745
BlsI GCNGC 2 cut(s) 86, 746
Bme1390I CCNGG 1 cut(s) 398
Bme18I GGWCC 1 cut(s) 737
BmeRI GACNNNNNGTC 1 cut(s) 688
BmgT120I GGNCC 1 cut(s) 737
BmiI GGNNCC 4 cut(s) 158, 225, 320, 616
BmrFI CCNGG 1 cut(s) 398
BmsI GCATC 1 cut(s) 552
BpiI GAAGAC 1 cut(s) 513
BpuMI CCSGG 1 cut(s) 398
Bsa29I ATCGAT 1 cut(s) 651
BsaI GGTCTC 1 cut(s) 173
BsaJI CCNNGG 1 cut(s) 363
BsaWI WCCGGW 1 cut(s) 617
Bsc4I CCNNNNNNNGG 1 cut(s) 572
Bse1I ACTGG 2 cut(s) 91, 569
BseCI ATCGAT 1 cut(s) 651
BseDI CCNNGG 1 cut(s) 363
BseGI GGATG 6 cut(s) 391, 487, 499, 567, 646, 726
BseLI CCNNNNNNNGG 1 cut(s) 572
BseNI ACTGG 2 cut(s) 91, 569
BseRI GAGGAG 3 cut(s) 192, 299, 701
BseX3I CGGCCG 1 cut(s) 82
BseXI GCAGC 1 cut(s) 756
BseYI CCCAGC 1 cut(s) 49
Bsh1285I CGRYCG 1 cut(s) 85
BshFI GGCC 3 cut(s) 84, 108, 122
BshNI GGYRCC 1 cut(s) 156
BshVI ATCGAT 1 cut(s) 651
BsiEI CGRYCG 1 cut(s) 85
BsiHKAI GWGCWC 4 cut(s) 75, 291, 702, 782
BsiSI CCGG 2 cut(s) 398, 618
BslFI GGGAC 1 cut(s) 599
BslI CCNNNNNNNGG 1 cut(s) 572
BsmAI GTCTC 3 cut(s) 95, 173, 417
BsmBI CGTCTC 1 cut(s) 417
BsmFI GGGAC 1 cut(s) 599
BsnI GGCC 3 cut(s) 84, 108, 122
Bso31I GGTCTC 1 cut(s) 173
Bsp1286I GDGCHC 5 cut(s) 56, 75, 291, 702, 782
Bsp143I GATC 3 cut(s) 223, 388, 648
BspACI CCGC 1 cut(s) 85
BspANI GGCC 3 cut(s) 84, 108, 122
BspCNI CTCAG 1 cut(s) 4
BspDI ATCGAT 1 cut(s) 651
BspLI GGNNCC 4 cut(s) 158, 225, 320, 616
BspMI ACCTGC 1 cut(s) 54
BspPI GGATC 2 cut(s) 218, 231
BspQI GCTCTTC 1 cut(s) 690
BspT107I GGYRCC 1 cut(s) 156
BspTNI GGTCTC 1 cut(s) 173
BsrI ACTGG 2 cut(s) 91, 569
BssECI CCNNGG 1 cut(s) 363
BssMI GATC 3 cut(s) 223, 388, 648
BssT1I CCWWGG 1 cut(s) 363
Bst4CI ACNGT 2 cut(s) 156, 318
Bst6I CTCTTC 5 cut(s) 330, 342, 639, 690, 761
BstAPI GCANNNNNTGC 1 cut(s) 156
BstC8I GCNNGC 2 cut(s) 56, 120
BstDEI CTNAG 1 cut(s) 12
BstF5I GGATG 6 cut(s) 391, 487, 499, 567, 646, 726
BstKTI GATC 3 cut(s) 226, 391, 651
BstMAI GTCTC 3 cut(s) 95, 173, 417
BstMBI GATC 3 cut(s) 223, 388, 648
BstMCI CGRYCG 1 cut(s) 85
BstMWI GCNNNNNNNGC 4 cut(s) 55, 93, 156, 665
BstNSI RCATGY 1 cut(s) 214
BstSCI CCNGG 1 cut(s) 396
BstV1I GCAGC 1 cut(s) 756
BstV2I GAAGAC 1 cut(s) 513
BstX2I RGATCY 1 cut(s) 223
BstXI CCANNNNNNTGG 1 cut(s) 370
BstYI RGATCY 1 cut(s) 223
BstZI CGGCCG 1 cut(s) 82
Bsu15I ATCGAT 1 cut(s) 651
BsuI GTATCC 1 cut(s) 259
BsuRI GGCC 3 cut(s) 84, 108, 122
BsuTUI ATCGAT 1 cut(s) 651
BtsCI GGATG 6 cut(s) 391, 487, 499, 567, 646, 726
BtsIMutI CAGTG 2 cut(s) 98, 576
BveI ACCTGC 1 cut(s) 54
Cac8I GCNNGC 2 cut(s) 56, 120
Cfr13I GGNCC 1 cut(s) 737
ClaI ATCGAT 1 cut(s) 651
CseI GACGC 1 cut(s) 85
Csp6I GTAC 1 cut(s) 407
CviAII CATG 2 cut(s) 211, 786
CviQI GTAC 1 cut(s) 407
DdeI CTNAG 1 cut(s) 12
DpnI GATC 3 cut(s) 225, 390, 650
DpnII GATC 3 cut(s) 223, 388, 648
DriI GACNNNNNGTC 1 cut(s) 688
EaeI YGGCCR 2 cut(s) 82, 120
EagI CGGCCG 1 cut(s) 82
Eam1104I CTCTTC 5 cut(s) 330, 342, 639, 690, 761
Eam1105I GACNNNNNGTC 1 cut(s) 688
EarI CTCTTC 5 cut(s) 330, 342, 639, 690, 761
Ecl136II GAGCTC 2 cut(s) 73, 700
EclXI CGGCCG 1 cut(s) 82
Eco130I CCWWGG 1 cut(s) 363
Eco24I GRGCYC 3 cut(s) 56, 75, 702
Eco31I GGTCTC 1 cut(s) 173
Eco47I GGWCC 1 cut(s) 737
Eco52I CGGCCG 1 cut(s) 82
Eco53kI GAGCTC 2 cut(s) 73, 700
Eco57I CTGAAG 1 cut(s) 564
EcoICRI GAGCTC 2 cut(s) 73, 700
EcoT14I CCWWGG 1 cut(s) 363
EcoT38I GRGCYC 3 cut(s) 56, 75, 702
ErhI CCWWGG 1 cut(s) 363
Esp3I CGTCTC 1 cut(s) 417
FaeI CATG 2 cut(s) 214, 789
FaiI YATR 9 cut(s) 131, 192, 212, 303, 305, 383, 503, 728, 787
FalI AAGNNNNNCTT 2 cut(s) 505, 537
FaqI GGGAC 1 cut(s) 599
FatI CATG 2 cut(s) 210, 785
FbaI TGATCA 1 cut(s) 388
Fnu4HI GCNGC 2 cut(s) 85, 745
FokI GGATG 6 cut(s) 398, 494, 506, 574, 653, 733
FriOI GRGCYC 3 cut(s) 56, 75, 702
Fsp4HI GCNGC 2 cut(s) 85, 745
GluI GCNGC 2 cut(s) 85, 745
GsaI CCCAGC 1 cut(s) 53
HaeIII GGCC 3 cut(s) 84, 108, 122
HapII CCGG 2 cut(s) 398, 618
HgaI GACGC 1 cut(s) 85
Hin1II CATG 2 cut(s) 214, 789
HinfI GANTC 2 cut(s) 626, 689
HpaII CCGG 2 cut(s) 398, 618
HphI GGTGA 2 cut(s) 548, 614
Hpy188I TCNGA 7 cut(s) 147, 283, 393, 526, 625, 631, 694
Hpy188III TCNNGA 2 cut(s) 100, 507
Hpy99I CGWCG 3 cut(s) 10, 101, 686
HpyAV CCTTC 7 cut(s) 10, 16, 29, 73, 334, 472, 527
HpyCH4III ACNGT 2 cut(s) 156, 318
HpyCH4IV ACGT 1 cut(s) 580
HpyCH4V TGCA 1 cut(s) 668
HpyF10VI GCNNNNNNNGC 4 cut(s) 55, 93, 156, 665
HpyF3I CTNAG 1 cut(s) 12
HpySE526I ACGT 1 cut(s) 580
Hsp92II CATG 2 cut(s) 214, 789
Ksp22I TGATCA 1 cut(s) 388
Kzo9I GATC 3 cut(s) 223, 388, 648
LguI GCTCTTC 1 cut(s) 690
LmnI GCTCC 6 cut(s) 39, 70, 147, 205, 286, 656
Lsp1109I GCAGC 1 cut(s) 756
LweI GCATC 1 cut(s) 552
MaeII ACGT 1 cut(s) 580
MaeIII GTNAC 1 cut(s) 602
MalI GATC 3 cut(s) 225, 390, 650
MboI GATC 3 cut(s) 223, 388, 648
MflI RGATCY 1 cut(s) 223
MhlI GDGCHC 5 cut(s) 56, 75, 291, 702, 782
MlsI TGGCCA 1 cut(s) 122
MluCI AATT 3 cut(s) 113, 135, 732
MluNI TGGCCA 1 cut(s) 122
MlyI GAGTC 2 cut(s) 635, 698
Mox20I TGGCCA 1 cut(s) 122
MroXI GAANNNNTTC 1 cut(s) 317
MscI TGGCCA 1 cut(s) 122
MslI CAYNNNNRTG 1 cut(s) 306
Msp20I TGGCCA 1 cut(s) 122
MspI CCGG 2 cut(s) 398, 618
MspR9I CCNGG 1 cut(s) 398
MwoI GCNNNNNNNGC 4 cut(s) 55, 93, 156, 665
NciI CCSGG 1 cut(s) 398
NdeII GATC 3 cut(s) 223, 388, 648
NlaIII CATG 2 cut(s) 214, 789
NlaIV GGNNCC 4 cut(s) 158, 225, 320, 616
NmuCI GTSAC 1 cut(s) 602
NspI RCATGY 1 cut(s) 214
PciI ACATGT 1 cut(s) 210
PciSI GCTCTTC 1 cut(s) 690
PdmI GAANNNNTTC 1 cut(s) 317
PkrI GCNGC 2 cut(s) 86, 746
PleI GAGTC 2 cut(s) 634, 697
PpsI GAGTC 2 cut(s) 634, 697
PscI ACATGT 1 cut(s) 210
Psp124BI GAGCTC 2 cut(s) 75, 702
Psp1406I AACGTT 1 cut(s) 580
PspFI CCCAGC 1 cut(s) 49
PspN4I GGNNCC 4 cut(s) 158, 225, 320, 616
PspPI GGNCC 1 cut(s) 737
PsuI RGATCY 1 cut(s) 223
RsaI GTAC 1 cut(s) 408
RsaNI GTAC 1 cut(s) 407
RseI CAYNNNNRTG 1 cut(s) 306
SacI GAGCTC 2 cut(s) 75, 702
SapI GCTCTTC 1 cut(s) 690
SatI GCNGC 2 cut(s) 85, 745
Sau3AI GATC 3 cut(s) 223, 388, 648
Sau96I GGNCC 1 cut(s) 737
SchI GAGTC 2 cut(s) 635, 698
ScrFI CCNGG 1 cut(s) 398
SduI GDGCHC 5 cut(s) 56, 75, 291, 702, 782
SfaNI GCATC 1 cut(s) 552
SinI GGWCC 1 cut(s) 737
SmiMI CAYNNNNRTG 1 cut(s) 306
Sse9I AATT 3 cut(s) 113, 135, 732
SsiI CCGC 1 cut(s) 85
SspI AATATT 1 cut(s) 179
SstI GAGCTC 2 cut(s) 75, 702
StyD4I CCNGG 1 cut(s) 396
StyI CCWWGG 1 cut(s) 363
TaaI ACNGT 2 cut(s) 156, 318
TaiI ACGT 1 cut(s) 583
TaqI TCGA 5 cut(s) 75, 99, 174, 333, 651
TasI AATT 3 cut(s) 113, 135, 732
TauI GCSGC 1 cut(s) 87
TscAI CASTG 2 cut(s) 98, 576
TseFI GTSAC 1 cut(s) 602
TseI GCWGC 1 cut(s) 744
Tsp45I GTSAC 1 cut(s) 602
TspDTI ATGAA 1 cut(s) 657
TspGWI ACGGA 2 cut(s) 435, 441
TspRI CASTG 2 cut(s) 98, 576
VpaK11BI GGWCC 1 cut(s) 737
XapI RAATTY 1 cut(s) 135
XceI RCATGY 1 cut(s) 214
XmnI GAANNNNTTC 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.