Rroxscaffold_2G00078880

Surfeit locus protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0007923
Physical Location & Seq
Reverse (-)
2040112 .. 2044919
4808 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00078880.1

Sequence Viewer

Length: 831 bp
ATGGCGACGAGTGAGGAGAAGACCAACAATACTAAAGAAGGGAACAACCTACTGGGAACGCCGACCTTCAAGGCCCTCGATAATGGTCGGTTCCAGTGCGTCGAGACTGGCCACCAATTACTGGCCAAAGACATTGAATCCTACTCTCAGAGCAAACGGTGCCGTTTGGGTCTGATCGAATTCGCACTCTCTCATAAGAAGCCTCCTCTCAACATGTTCAAGCAGGACCCTCTTTCTCGTTCAAAGTTGGTGTGTAAGCTAACCGAGGATTCCATCAATAAGTCAGAGGAACACATTTGGAAGCATATTAATGGGAAACGATTCCTCAACAAACTGGAAGAAATGGAAGAACAAAAGCTAACAGTAAATGAAATGGAAGAAGAGCTGGATGATCACAAACCTGAAAAAGTATCTAAGCCAGGCAAAGATGGTGCCAGGAAGGAGAAAAAGGAGAAGAAAAAGAAGAAGAAGAAGGAGGAGGCAAAGGAGGAGGATGGTGGTGATGATGAGATTATCTCTGAAATACCCAAGTCTCCTAGTGAGGAAAATGATTCTGAAGAAGCTGAGTTTTGGATCCCTCCACCAGGAGCACGTTGGGATTTTGATGATGGGGGTGACAGATGGGGTTCCGATTCAGAGTCAGAGAGCAATGACGAGAACAAAAGTGGCGAGGCTGATGGAATTGTTGAAGATGATGATGAGGAGTCAGAAGAGATCTCAATGCGGTATGCCACTGCTTCACTATGTTCTATTGGTGGAAGAGCCAATAAGCATCCTGGTTTTCTTTCTTTTGGAAACAAATTTATGCCATTTTCTTTTGAATCTGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SURF2

Protein Analysis

276

Amino Acids

31.25

Weight (kDa)

4.94

Isoelectric Point (pI)

65.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SURF2 PF05477 12 - 238 2.7e-51 Surfeit locus protein 2 (SURF2)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 159, 431
AccB7I CCANNNNNTGG 1 cut(s) 121
AciI CCGC 1 cut(s) 724
AclWI GGATC 2 cut(s) 568, 581
AcoI YGGCCR 2 cut(s) 109, 123
AcsI RAATTY 2 cut(s) 179, 800
AcuI CTGAAG 1 cut(s) 576
AfiI CCNNNNNNNGG 2 cut(s) 121, 584
AflIII ACRYGT 1 cut(s) 213
AgsI TTSAA 6 cut(s) 70, 137, 220, 243, 689, 821
AjnI CCWGG 4 cut(s) 418, 434, 583, 775
AluBI AGCT 4 cut(s) 259, 358, 385, 563
AluI AGCT 4 cut(s) 259, 358, 385, 563
Alw21I GWGCWC 1 cut(s) 592
Alw26I GTCTC 2 cut(s) 98, 537
AlwI GGATC 2 cut(s) 568, 581
AoxI GGCC 3 cut(s) 72, 109, 123
ApoI RAATTY 2 cut(s) 179, 800
AseI ATTAAT 1 cut(s) 309
Asp700I GAANNNNTTC 1 cut(s) 320
AspS9I GGNCC 2 cut(s) 73, 226
AsuHPI GGTGA 2 cut(s) 512, 626
AvaII GGWCC 1 cut(s) 226
BalI TGGCCA 2 cut(s) 111, 125
BamHI GGATCC 1 cut(s) 573
BanI GGYRCC 2 cut(s) 159, 431
BbsI GAAGAC 1 cut(s) 26
Bbv12I GWGCWC 1 cut(s) 592
BccI CCATC 6 cut(s) 281, 422, 488, 602, 615, 671
BceAI ACGGC 1 cut(s) 147
BciT130I CCWGG 4 cut(s) 420, 436, 585, 777
BclI TGATCA 1 cut(s) 391
BcoDI GTCTC 2 cut(s) 98, 537
BfaI CTAG 1 cut(s) 537
BglII AGATCT 1 cut(s) 714
Bme1390I CCNGG 4 cut(s) 420, 436, 585, 777
Bme18I GGWCC 1 cut(s) 226
BmgT120I GGNCC 2 cut(s) 73, 226
BmiI GGNNCC 6 cut(s) 92, 161, 228, 433, 575, 628
BmrFI CCNGG 4 cut(s) 420, 436, 585, 777
BmrI ACTGGG 1 cut(s) 62
BmsI GCATC 1 cut(s) 781
BmuI ACTGGG 1 cut(s) 62
BpiI GAAGAC 1 cut(s) 26
BplI GAGNNNNNCTC 2 cut(s) 500, 532
BsaJI CCNNGG 1 cut(s) 264
Bsc4I CCNNNNNNNGG 2 cut(s) 121, 584
Bse1I ACTGG 5 cut(s) 57, 94, 112, 126, 339
Bse3DI GCAATG 1 cut(s) 655
BseBI CCWGG 4 cut(s) 420, 436, 585, 777
BseDI CCNNGG 1 cut(s) 264
BseGI GGATG 3 cut(s) 394, 499, 772
BseLI CCNNNNNNNGG 2 cut(s) 121, 584
BseMI GCAATG 1 cut(s) 655
BseMII CTCAG 2 cut(s) 161, 555
BseNI ACTGG 5 cut(s) 57, 94, 112, 126, 339
BseRI GAGGAG 5 cut(s) 29, 195, 491, 503, 716
BshFI GGCC 3 cut(s) 74, 111, 125
BshNI GGYRCC 2 cut(s) 159, 431
BsiHKAI GWGCWC 1 cut(s) 592
BslI CCNNNNNNNGG 2 cut(s) 121, 584
BsmAI GTCTC 2 cut(s) 98, 537
BsnI GGCC 3 cut(s) 74, 111, 125
Bsp1286I GDGCHC 1 cut(s) 592
Bsp143I GATC 4 cut(s) 174, 391, 573, 714
BspACI CCGC 1 cut(s) 724
BspANI GGCC 3 cut(s) 74, 111, 125
BspCNI CTCAG 2 cut(s) 160, 556
BspLI GGNNCC 6 cut(s) 92, 161, 228, 433, 575, 628
BspPI GGATC 2 cut(s) 568, 581
BspQI GCTCTTC 2 cut(s) 375, 754
BspT107I GGYRCC 2 cut(s) 159, 431
BsrDI GCAATG 1 cut(s) 655
BsrI ACTGG 5 cut(s) 57, 94, 112, 126, 339
BssECI CCNNGG 1 cut(s) 264
BssMI GATC 4 cut(s) 174, 391, 573, 714
Bst2UI CCWGG 4 cut(s) 420, 436, 585, 777
Bst4CI ACNGT 2 cut(s) 159, 364
Bst6I CTCTTC 3 cut(s) 375, 705, 754
BstAPI GCANNNNNTGC 1 cut(s) 159
BstDEI CTNAG 3 cut(s) 147, 414, 564
BstENI CCTNNNNNAGG 1 cut(s) 582
BstF5I GGATG 3 cut(s) 394, 499, 772
BstKTI GATC 4 cut(s) 177, 394, 576, 717
BstMAI GTCTC 2 cut(s) 98, 537
BstMBI GATC 4 cut(s) 174, 391, 573, 714
BstMWI GCNNNNNNNGC 1 cut(s) 159
BstNI CCWGG 4 cut(s) 420, 436, 585, 777
BstNSI RCATGY 1 cut(s) 217
BstSCI CCNGG 4 cut(s) 418, 434, 583, 775
BstV2I GAAGAC 1 cut(s) 26
BstX2I RGATCY 2 cut(s) 573, 714
BstYI RGATCY 2 cut(s) 573, 714
BsuRI GGCC 3 cut(s) 74, 111, 125
BtsCI GGATG 3 cut(s) 394, 499, 772
BtsI GCAGTG 1 cut(s) 732
BtsIMutI CAGTG 2 cut(s) 101, 732
Cfr13I GGNCC 2 cut(s) 73, 226
CseI GACGC 1 cut(s) 88
CviAII CATG 1 cut(s) 214
DdeI CTNAG 3 cut(s) 147, 414, 564
DpnI GATC 4 cut(s) 176, 393, 575, 716
DpnII GATC 4 cut(s) 174, 391, 573, 714
EaeI YGGCCR 2 cut(s) 109, 123
Eam1104I CTCTTC 3 cut(s) 375, 705, 754
EarI CTCTTC 3 cut(s) 375, 705, 754
Eco47I GGWCC 1 cut(s) 226
Eco57I CTGAAG 1 cut(s) 576
EcoNI CCTNNNNNAGG 1 cut(s) 582
EcoO109I RGGNCCY 2 cut(s) 73, 226
EcoRI GAATTC 1 cut(s) 179
EcoRII CCWGG 4 cut(s) 418, 434, 583, 775
FaeI CATG 1 cut(s) 217
FaiI YATR 6 cut(s) 195, 215, 306, 729, 745, 806
FatI CATG 1 cut(s) 213
FbaI TGATCA 1 cut(s) 391
FokI GGATG 3 cut(s) 401, 506, 759
FspBI CTAG 1 cut(s) 537
HaeIII GGCC 3 cut(s) 74, 111, 125
HgaI GACGC 1 cut(s) 88
Hin1II CATG 1 cut(s) 217
HinfI GANTC 8 cut(s) 137, 269, 321, 551, 632, 638, 704, 821
HphI GGTGA 2 cut(s) 512, 626
Hpy188I TCNGA 9 cut(s) 150, 174, 286, 520, 556, 631, 637, 643, 709
Hpy188III TCNNGA 1 cut(s) 103
Hpy99I CGWCG 2 cut(s) 10, 104
HpyAV CCTTC 4 cut(s) 32, 76, 433, 466
HpyCH4III ACNGT 2 cut(s) 159, 364
HpyCH4IV ACGT 1 cut(s) 592
HpyF10VI GCNNNNNNNGC 1 cut(s) 159
HpyF3I CTNAG 3 cut(s) 147, 414, 564
HpySE526I ACGT 1 cut(s) 592
Hsp92II CATG 1 cut(s) 217
Ksp22I TGATCA 1 cut(s) 391
Kzo9I GATC 4 cut(s) 174, 391, 573, 714
LguI GCTCTTC 2 cut(s) 375, 754
LmnI GCTCC 1 cut(s) 587
LweI GCATC 1 cut(s) 781
MaeI CTAG 1 cut(s) 537
MaeII ACGT 1 cut(s) 592
MaeIII GTNAC 1 cut(s) 614
MalI GATC 4 cut(s) 176, 393, 575, 716
MboI GATC 4 cut(s) 174, 391, 573, 714
MflI RGATCY 2 cut(s) 573, 714
MhlI GDGCHC 1 cut(s) 592
MlsI TGGCCA 2 cut(s) 111, 125
MluCI AATT 4 cut(s) 116, 179, 681, 800
MluNI TGGCCA 2 cut(s) 111, 125
MlyI GAGTC 2 cut(s) 647, 713
Mox20I TGGCCA 2 cut(s) 111, 125
MroXI GAANNNNTTC 1 cut(s) 320
MscI TGGCCA 2 cut(s) 111, 125
MseI TTAA 1 cut(s) 309
MslI CAYNNNNRTG 1 cut(s) 309
Msp20I TGGCCA 2 cut(s) 111, 125
MspR9I CCNGG 4 cut(s) 420, 436, 585, 777
MvaI CCWGG 4 cut(s) 420, 436, 585, 777
MwoI GCNNNNNNNGC 1 cut(s) 159
NdeII GATC 4 cut(s) 174, 391, 573, 714
NlaIII CATG 1 cut(s) 217
NlaIV GGNNCC 6 cut(s) 92, 161, 228, 433, 575, 628
NmuCI GTSAC 1 cut(s) 614
NspI RCATGY 1 cut(s) 217
PciI ACATGT 1 cut(s) 213
PciSI GCTCTTC 2 cut(s) 375, 754
PdmI GAANNNNTTC 1 cut(s) 320
PfeI GAWTC 6 cut(s) 137, 269, 321, 551, 632, 821
PflMI CCANNNNNTGG 1 cut(s) 121
PleI GAGTC 2 cut(s) 646, 712
PpsI GAGTC 2 cut(s) 646, 712
PpuMI RGGWCCY 1 cut(s) 226
PscI ACATGT 1 cut(s) 213
PshBI ATTAAT 1 cut(s) 309
Psp5II RGGWCCY 1 cut(s) 226
Psp6I CCWGG 4 cut(s) 418, 434, 583, 775
PspGI CCWGG 4 cut(s) 418, 434, 583, 775
PspN4I GGNNCC 6 cut(s) 92, 161, 228, 433, 575, 628
PspPI GGNCC 2 cut(s) 73, 226
PspPPI RGGWCCY 1 cut(s) 226
PsuI RGATCY 2 cut(s) 573, 714
RseI CAYNNNNRTG 1 cut(s) 309
SapI GCTCTTC 2 cut(s) 375, 754
SaqAI TTAA 1 cut(s) 309
Sau3AI GATC 4 cut(s) 174, 391, 573, 714
Sau96I GGNCC 2 cut(s) 73, 226
SchI GAGTC 2 cut(s) 647, 713
ScrFI CCNGG 4 cut(s) 420, 436, 585, 777
SduI GDGCHC 1 cut(s) 592
SetI ASST 8 cut(s) 51, 68, 261, 360, 387, 403, 565, 595
SfaNI GCATC 1 cut(s) 781
SinI GGWCC 1 cut(s) 226
SmiMI CAYNNNNRTG 1 cut(s) 309
Sse9I AATT 4 cut(s) 116, 179, 681, 800
SsiI CCGC 1 cut(s) 724
SspMI CTAG 1 cut(s) 537
StyD4I CCNGG 4 cut(s) 418, 434, 583, 775
TaaI ACNGT 2 cut(s) 159, 364
TaiI ACGT 1 cut(s) 595
TaqI TCGA 3 cut(s) 78, 102, 177
TasI AATT 4 cut(s) 116, 179, 681, 800
TfiI GAWTC 6 cut(s) 137, 269, 321, 551, 632, 821
Tru1I TTAA 1 cut(s) 309
Tru9I TTAA 1 cut(s) 309
TscAI CASTG 2 cut(s) 101, 739
TseFI GTSAC 1 cut(s) 614
Tsp45I GTSAC 1 cut(s) 614
TspDTI ATGAA 1 cut(s) 384
TspRI CASTG 2 cut(s) 101, 739
Van91I CCANNNNNTGG 1 cut(s) 121
VpaK11BI GGWCC 1 cut(s) 226
VspI ATTAAT 1 cut(s) 309
XagI CCTNNNNNAGG 1 cut(s) 582
XapI RAATTY 2 cut(s) 179, 800
XceI RCATGY 1 cut(s) 217
XcmI CCANNNNNNNNNTGG 1 cut(s) 591
XmnI GAANNNNTTC 1 cut(s) 320
XspI CTAG 1 cut(s) 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.