MD17G1021600.v1.1

Surfeit locus protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0007923
Physical Location & Seq
Reverse (-)
1614478 .. 1616447
1970 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1021600.v1.1.491

Sequence Viewer

Length: 789 bp
ATGGCGACGACTGAGAAGAAGGCGGAGGAGGAGGAGAAGGAAGGGAGCAACCTACTGGGCTCGCCGACCTTCAAGGAGCTCGAAAATGGCCGCTTCCAGTGCGTGGAGACAGGCCACGAATTGGTGGCCAAAGACAAGGGAGTGTACTCTCAGAGCAAACGGTGCCGTTTGGGCCTAATCGAATATGCCCTTTCCCATGAGAAAGCTCCTCTCATCATGTTCAAGCAGGATCCTCTTTCCCGTTCAAAGTTGGTGTGTAAGCTAACGGGGGATACTATCAATAAGTCAGAGGAGCACATTTGGAAGCATATAAATGGGAAACGCTTCCTCAACAAGCTCGAAGAGAAGGAAGAAGAGAAGCTAAAAGCCAAGGGAGCGGTAGAAGAGATGGATGATCAGAACCCGGAAATAGTATCTGACCAAGAAGAAGACGGAGACAGAAAGAAGAAGAAGAAGAATAAGAATAAGAAGAAGGATGATGGAGTTGATGAGATTATGTCTGGAAAAAGTAAGGCTTCTGATGAGGAAGGCGACACAGAAGAAACTGACTTTTGGATGCCTCCAGTGGGAGAACGTTGGGACTTTGACGATGGAGGTGATAGGTGGGGTTCTGGTTCAGAGTCAGAGGACGAGGATGACAAGATTGATGGAGCTGTTTCAGATGGTGCATTTGAAGATGGCGACGAGGAGTCAGAAGAGCTCTCCGAGCGGACGAAAAGAATGTCCATAGAAATTGGACCGAGCAGCTTTGCTTCGAGGAAGAAGAAGAGTAAAAAGAACACGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SURF2

Protein Analysis

263

Amino Acids

29.63

Weight (kDa)

4.91

Isoelectric Point (pI)

65.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SURF2 PF05477 12 - 258 5.9e-59 Surfeit locus protein 2 (SURF2)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 162
AccB7I CCANNNNNTGG 2 cut(s) 103, 121
AccBSI CCGCTC 2 cut(s) 377, 709
AciI CCGC 4 cut(s) 23, 91, 377, 709
AclI AACGTT 1 cut(s) 574
AclWI GGATC 2 cut(s) 224, 237
AcoI YGGCCR 2 cut(s) 88, 126
AfaI GTAC 1 cut(s) 146
AfiI CCNNNNNNNGG 3 cut(s) 103, 121, 566
AgsI TTSAA 4 cut(s) 73, 223, 246, 674
AhdI GACNNNNNGTC 1 cut(s) 688
AluBI AGCT 8 cut(s) 79, 206, 262, 337, 361, 653, 700, 747
AluI AGCT 8 cut(s) 79, 206, 262, 337, 361, 653, 700, 747
Alw21I GWGCWC 3 cut(s) 81, 297, 702
Alw26I GTCTC 2 cut(s) 101, 429
AlwI GGATC 2 cut(s) 224, 237
AoxI GGCC 4 cut(s) 88, 112, 126, 172
ApeKI GCWGC 1 cut(s) 744
ArsI GACNNNNNNTTYG 2 cut(s) 707, 739
Asp700I GAANNNNTTC 1 cut(s) 323
AspS9I GGNCC 2 cut(s) 172, 737
AsuC2I CCSGG 1 cut(s) 404
AsuHPI GGTGA 1 cut(s) 608
AvaII GGWCC 1 cut(s) 737
BalI TGGCCA 1 cut(s) 128
BamHI GGATCC 1 cut(s) 229
BanI GGYRCC 1 cut(s) 162
BanII GRGCYC 3 cut(s) 62, 81, 702
BbsI GAAGAC 1 cut(s) 435
Bbv12I GWGCWC 3 cut(s) 81, 297, 702
BbvI GCAGC 1 cut(s) 756
BccI CCATC 6 cut(s) 382, 473, 584, 641, 656, 671
BceAI ACGGC 1 cut(s) 150
BciVI GTATCC 1 cut(s) 265
BclI TGATCA 1 cut(s) 394
BcnI CCSGG 1 cut(s) 404
BcoDI GTCTC 2 cut(s) 101, 429
BfuI GTATCC 1 cut(s) 265
BglI GCCNNNNNGGC 1 cut(s) 171
BisI GCNGC 2 cut(s) 91, 745
BlsI GCNGC 2 cut(s) 92, 746
Bme1390I CCNGG 1 cut(s) 404
Bme18I GGWCC 1 cut(s) 737
BmeRI GACNNNNNGTC 1 cut(s) 688
BmgT120I GGNCC 2 cut(s) 172, 737
BmiI GGNNCC 2 cut(s) 164, 231
BmrFI CCNGG 1 cut(s) 404
BmrI ACTGGG 1 cut(s) 65
BmsI GCATC 1 cut(s) 546
BmuI ACTGGG 1 cut(s) 65
BpiI GAAGAC 1 cut(s) 435
BpmI CTGGAG 1 cut(s) 546
BpuMI CCSGG 1 cut(s) 404
BsaJI CCNNGG 1 cut(s) 369
Bsc4I CCNNNNNNNGG 3 cut(s) 103, 121, 566
Bse1I ACTGG 3 cut(s) 60, 97, 563
BseDI CCNNGG 1 cut(s) 369
BseGI GGATG 4 cut(s) 397, 481, 561, 640
BseLI CCNNNNNNNGG 3 cut(s) 103, 121, 566
BseMII CTCAG 1 cut(s) 164
BseNI ACTGG 3 cut(s) 60, 97, 563
BseRI GAGGAG 6 cut(s) 41, 44, 47, 198, 305, 701
BseXI GCAGC 1 cut(s) 756
BshFI GGCC 4 cut(s) 90, 114, 128, 174
BshNI GGYRCC 1 cut(s) 162
BsiHKAI GWGCWC 3 cut(s) 81, 297, 702
BsiSI CCGG 1 cut(s) 404
BslFI GGGAC 1 cut(s) 593
BslI CCNNNNNNNGG 3 cut(s) 103, 121, 566
BsmAI GTCTC 2 cut(s) 101, 429
BsmFI GGGAC 1 cut(s) 593
BsnI GGCC 4 cut(s) 90, 114, 128, 174
Bsp1286I GDGCHC 4 cut(s) 62, 81, 297, 702
Bsp143I GATC 2 cut(s) 229, 394
BspACI CCGC 4 cut(s) 23, 91, 377, 709
BspANI GGCC 4 cut(s) 90, 114, 128, 174
BspCNI CTCAG 2 cut(s) 4, 163
BspLI GGNNCC 2 cut(s) 164, 231
BspPI GGATC 2 cut(s) 224, 237
BspQI GCTCTTC 1 cut(s) 690
BspT107I GGYRCC 1 cut(s) 162
BsrBI CCGCTC 2 cut(s) 377, 709
BsrI ACTGG 3 cut(s) 60, 97, 563
BssECI CCNNGG 1 cut(s) 369
BssMI GATC 2 cut(s) 229, 394
BssT1I CCWWGG 1 cut(s) 369
Bst4CI ACNGT 1 cut(s) 162
Bst6I CTCTTC 5 cut(s) 336, 348, 378, 690, 761
BstAPI GCANNNNNTGC 1 cut(s) 162
BstC8I GCNNGC 1 cut(s) 62
BstDEI CTNAG 2 cut(s) 12, 150
BstF5I GGATG 4 cut(s) 397, 481, 561, 640
BstKTI GATC 2 cut(s) 232, 397
BstMAI GTCTC 2 cut(s) 101, 429
BstMBI GATC 2 cut(s) 229, 394
BstMWI GCNNNNNNNGC 5 cut(s) 99, 162, 171, 374, 706
BstSCI CCNGG 1 cut(s) 402
BstV1I GCAGC 1 cut(s) 756
BstV2I GAAGAC 1 cut(s) 435
BstX2I RGATCY 1 cut(s) 229
BstYI RGATCY 1 cut(s) 229
BsuI GTATCC 1 cut(s) 265
BsuRI GGCC 4 cut(s) 90, 114, 128, 174
BtsCI GGATG 4 cut(s) 397, 481, 561, 640
BtsIMutI CAGTG 2 cut(s) 104, 570
Cac8I GCNNGC 1 cut(s) 62
Cfr13I GGNCC 2 cut(s) 172, 737
Csp6I GTAC 1 cut(s) 145
CviAII CATG 3 cut(s) 197, 217, 786
CviQI GTAC 1 cut(s) 145
DdeI CTNAG 2 cut(s) 12, 150
DpnI GATC 2 cut(s) 231, 396
DpnII GATC 2 cut(s) 229, 394
DriI GACNNNNNGTC 1 cut(s) 688
EaeI YGGCCR 2 cut(s) 88, 126
Eam1104I CTCTTC 5 cut(s) 336, 348, 378, 690, 761
Eam1105I GACNNNNNGTC 1 cut(s) 688
EarI CTCTTC 5 cut(s) 336, 348, 378, 690, 761
EciI GGCGGA 1 cut(s) 38
Ecl136II GAGCTC 2 cut(s) 79, 700
Eco130I CCWWGG 1 cut(s) 369
Eco24I GRGCYC 3 cut(s) 62, 81, 702
Eco47I GGWCC 1 cut(s) 737
Eco53kI GAGCTC 2 cut(s) 79, 700
EcoICRI GAGCTC 2 cut(s) 79, 700
EcoT14I CCWWGG 1 cut(s) 369
EcoT38I GRGCYC 3 cut(s) 62, 81, 702
ErhI CCWWGG 1 cut(s) 369
FaeI CATG 3 cut(s) 200, 220, 789
FaiI YATR 8 cut(s) 186, 198, 218, 309, 311, 497, 728, 787
FalI AAGNNNNNCTT 2 cut(s) 499, 531
FaqI GGGAC 1 cut(s) 593
FatI CATG 3 cut(s) 196, 216, 785
FbaI TGATCA 1 cut(s) 394
Fnu4HI GCNGC 2 cut(s) 91, 745
FokI GGATG 4 cut(s) 404, 488, 568, 647
FriOI GRGCYC 3 cut(s) 62, 81, 702
Fsp4HI GCNGC 2 cut(s) 91, 745
GluI GCNGC 2 cut(s) 91, 745
GsuI CTGGAG 1 cut(s) 546
HaeIII GGCC 4 cut(s) 90, 114, 128, 174
HapII CCGG 1 cut(s) 404
Hin1II CATG 3 cut(s) 200, 220, 789
HinfI GANTC 2 cut(s) 620, 689
HpaII CCGG 1 cut(s) 404
HphI GGTGA 1 cut(s) 608
Hpy166II GTNNAC 1 cut(s) 145
Hpy188III TCNNGA 1 cut(s) 501
Hpy8I GTNNAC 1 cut(s) 145
Hpy99I CGWCG 2 cut(s) 10, 686
HpyAV CCTTC 7 cut(s) 13, 31, 35, 79, 340, 466, 521
HpyCH4III ACNGT 1 cut(s) 162
HpyCH4IV ACGT 1 cut(s) 574
HpyCH4V TGCA 1 cut(s) 668
HpyF10VI GCNNNNNNNGC 5 cut(s) 99, 162, 171, 374, 706
HpyF3I CTNAG 2 cut(s) 12, 150
HpySE526I ACGT 1 cut(s) 574
Hsp92II CATG 3 cut(s) 200, 220, 789
Ksp22I TGATCA 1 cut(s) 394
Kzo9I GATC 2 cut(s) 229, 394
LguI GCTCTTC 1 cut(s) 690
LmnI GCTCC 6 cut(s) 45, 76, 211, 292, 374, 650
LpnPI CCDG 8 cut(s) 41, 96, 110, 212, 417, 486, 576, 597
Lsp1109I GCAGC 1 cut(s) 756
LweI GCATC 1 cut(s) 546
MaeII ACGT 1 cut(s) 574
MalI GATC 2 cut(s) 231, 396
MbiI CCGCTC 2 cut(s) 377, 709
MboI GATC 2 cut(s) 229, 394
MflI RGATCY 1 cut(s) 229
MhlI GDGCHC 4 cut(s) 62, 81, 297, 702
MlsI TGGCCA 1 cut(s) 128
MluCI AATT 2 cut(s) 119, 732
MluNI TGGCCA 1 cut(s) 128
MlyI GAGTC 2 cut(s) 629, 698
Mox20I TGGCCA 1 cut(s) 128
MroXI GAANNNNTTC 1 cut(s) 323
MscI TGGCCA 1 cut(s) 128
MslI CAYNNNNRTG 1 cut(s) 312
Msp20I TGGCCA 1 cut(s) 128
MspI CCGG 1 cut(s) 404
MspR9I CCNGG 1 cut(s) 404
MwoI GCNNNNNNNGC 5 cut(s) 99, 162, 171, 374, 706
NciI CCSGG 1 cut(s) 404
NdeII GATC 2 cut(s) 229, 394
NlaIII CATG 3 cut(s) 200, 220, 789
NlaIV GGNNCC 2 cut(s) 164, 231
PciSI GCTCTTC 1 cut(s) 690
PdmI GAANNNNTTC 1 cut(s) 323
PflMI CCANNNNNTGG 2 cut(s) 103, 121
PkrI GCNGC 2 cut(s) 92, 746
PleI GAGTC 2 cut(s) 628, 697
PpsI GAGTC 2 cut(s) 628, 697
Psp124BI GAGCTC 2 cut(s) 81, 702
Psp1406I AACGTT 1 cut(s) 574
PspN4I GGNNCC 2 cut(s) 164, 231
PspPI GGNCC 2 cut(s) 172, 737
PsuI RGATCY 1 cut(s) 229
RsaI GTAC 1 cut(s) 146
RsaNI GTAC 1 cut(s) 145
RseI CAYNNNNRTG 1 cut(s) 312
SacI GAGCTC 2 cut(s) 81, 702
SapI GCTCTTC 1 cut(s) 690
SatI GCNGC 2 cut(s) 91, 745
Sau3AI GATC 2 cut(s) 229, 394
Sau96I GGNCC 2 cut(s) 172, 737
SchI GAGTC 2 cut(s) 629, 698
ScrFI CCNGG 1 cut(s) 404
SduI GDGCHC 4 cut(s) 62, 81, 297, 702
SfaNI GCATC 1 cut(s) 546
SinI GGWCC 1 cut(s) 737
SmiMI CAYNNNNRTG 1 cut(s) 312
Sse9I AATT 2 cut(s) 119, 732
SsiI CCGC 4 cut(s) 23, 91, 377, 709
SstI GAGCTC 2 cut(s) 81, 702
StyD4I CCNGG 1 cut(s) 402
StyI CCWWGG 1 cut(s) 369
TaaI ACNGT 1 cut(s) 162
TaiI ACGT 1 cut(s) 577
TaqI TCGA 4 cut(s) 81, 180, 339, 755
TaqII GACCGA 1 cut(s) 754
TasI AATT 2 cut(s) 119, 732
TatI WGTACW 1 cut(s) 144
TauI GCSGC 1 cut(s) 93
TscAI CASTG 2 cut(s) 104, 570
TseI GCWGC 1 cut(s) 744
TspGWI ACGGA 1 cut(s) 447
TspRI CASTG 2 cut(s) 104, 570
Van91I CCANNNNNTGG 2 cut(s) 103, 121
VpaK11BI GGWCC 1 cut(s) 737
XmnI GAANNNNTTC 1 cut(s) 323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.