Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0002988

Physical Location & Seq

Genomic Coordinates Forward (+)

4248652 .. 4255033

Gene Structure 6382 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1062600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3018 bp

ATGGCAGCTTCCCAATTCTCTGCAACTCGCAGCGGAGCAGAGACAGCGTGGCATTGCAAATCGCAATCCAAACTGATCGATTTCGGTTCCAGGAAGAACAAATCCAAGCTTTTGTTCACAAGGACATTGAATCGGACTCGGCCGTTTTCGTTCTCGGTAAAGAGCGTTCTGGACAAGCCTCACGAGCTCAAGAATCCGATCATCGAACAAGATGCTGCAAGCGAATTCAGCTCATTCGTACCTGATGCTGCCTCCATCGCTTCGAGCATCAAATACCACGCAGAATTCACCCCGTTGTTTTCTCCGGAGAAGTTTGAGCTTCCAAAGGCCTTCTTTGCAACTGCACAAAGTGTTCGTGATGCGCTTATTGTTAACTGGAATGCAACAAATAATCATTATGAAAAATTGAACGCAAAGCAGGCATATTATTTGTCAATGGAATTTCTACAGGGTAGAGCGCTGTTAAATGCCATTGGTAATTTAGAGCTTGATGGTGCATATGCGGAAGCTTTAAGCAAGCTTGGACACAAATTAGAAAATGTTGCTAACCAGGAGCCAGATGCTGCACTTGGAAACGGAGGTCTTGGTCGGCTTGCCTCTTGCTTCTTGGACTCTTTGGCAACGTTAAATTATCCAGCATGGGGTTATGGGCTTCGGTACAAGTATGGCTTATTTAAACAGCTGATTACTAAAGATGGTCAAGAAGAAGTTGCTGAGGATTGGCTTGAGATGGGAAATCCCTGGGAGATTGTGAGAAATGATGTCTCCTATCCTGTCAAATTTTATGGCAAGATTGTTACTGGTTCGGATGGAAAACAGCATTGGATTGGAGGAGAAGACATAGATGCTGTTGCTTATGATGTTCCAATACCTGGATACAAAACTAAAACCACAATCAACTTGCGGCTTTGGTCAACGAAAGCTTCATCACAGAACTTTGATTTATATGCTTTTAATTCTGGAGAGCACACGAAAGCATCTGAAGCTTTAGCAAATGCTGAAAAGATTTGCTACGTACTTTATCCTGGGGATGAATCAATGGAGGGCAAGACACTTCGTTTGAAGCAACAATATACTTTATGTTCTGCTTCTCTCCAAGATATTGTTGCACGTTTTGAGAGAAGATCTGGAGCAAATGTCAAATGGGAAGAGTTCCCCGAGAAGGTGGCAGTGCAGATGAATGATACTCATCCAACTCTCTGCATTCCTGAACTGATGAGAATTTTGATTGATTTGAAGGGTTTGGACTGGAAGGAGGCCTGGAGTATTACTCAAAGGACGGTTGCATATACAAACCATACTGTTCTACCTGAGGCCTTGGAGAAATGGAGTCTGGAACTTATGGAGAAGCTGCTTCCTCGACATGTTCAGATCATACAGATGATAGATGAGGAGCTCATTCAGACCATAATTTCGGAATATGGCACAGCAGATTATGATTTATTAGAGAAGAAACTTAAGGAGATGAGAATATTAGAAAATGTTGACTTGCCTGCCAAATTTTCAGATTTAATTGTTAAACCCGAAAAGAGTTCCACTGCTGTCCCGAGTGAAGAAATTGAAAAGTCAGAAGAGGAAGATGAATCTGCTGATGCCGAAAAGAGTTCCACTGCTGTCCAGAGTGAAGAAATTGAAGAGTCAGAAGAGGAAGATGAATCTGCTGATGCCGAAAAGAGTTCCACTGCTGTCCTGAGTGAAGAAATTGAAGAGTCAGAAGAGGAAGGTGAATCTGCTGATGAAGAAAAAGTACCTGTGAAGAAACGCGAAGAGGAAAAAAAGAAAAAGGTTGTGGTGGAACCACCACCGAAGTTAGTACGTATGGCGAATCTCTGTGTTGTGGGTGGTCACGCAGTAAATGGCGTTGCTGAAATACACAGTGAAATAGTGAAAGATGAAGTATTTAATTCATTTTATAAGTTGTGGCCTGATAAATTTCAAAACAAAACAAATGGGGTGACACCAAGAAGATGGATCCGCTTCTGTAATCCAGATTTAAGTAACATTATAACAAAGTGGATTGGCACGGAAGACTGGGTCTTAAATACTGAAAAACTGGCCGAATTACGAAAGTTTGCAGACAATCAGGATCTCCAAACCCAATGGAGGGAAGCAAAAAGGAACAACAAGTTGAAAGTTGTGTCCTTGATCAAAGAAAGAACGGGTTATTCTGTCAACCCTGATGCAATGTTTGATATACAGGTGAAGCGCATTCATGAATACAAGCGACAACTGATGAATATTATGGGAATTGTTTACCGCTACAAGAAAATGAAAGAAATGAGTGCTTCAGGAAGGAAATCAAAGTTTGTTCCACGTGTTTGTATGTTTGGAGGAAAAGCATTTTCTACGTATGTGCAAGCCAAGAGAATTGTGAAATTTATCGCAGATGTAGGGGCAACCATTAATCATGATCCTAGTATCGGTGATCTACTGAAGGTTGTCTTCGTCCCCGATTACAATGTCAGTGTTGCTGAACAGTTAATTCCTGCAAGTGAGCTCTCACAGCACATCAGTACCGCTGGTATGGAAGCCAGTGGAACCAGCAACATGAAGTTTGCAATGAATGGCTGCATCCTCATTGGGACTCTAGACGGTGCCAATGTTGAGATAAGAGAAGAGGTTGGGGAAGACAACTTTTTCCTCTTTGGTGCTAAAGCTCATGAGATTGCTGGGCTGAGAAAGGAACGAGCTGAGGGAAAGTTTGTTCCGGACCCACGTTTTGAAGAAGTCAAGGAATTTGTCAAAAGCGGTGTTTTTGGGTCGTACAATTATGACGAACTGATTGGATCCCTGGAAGGAAATGAAGGATTTGGCCAGGCAGATTATTTCCTTGTGGGCAAGGACTTCCCCAGTTACATAGAATGTCAAGAGAAGGTCGATGAAGCATATCGAGACCAAAAGAGATGGACAAGAATGTCGATCCTGAACACAGCAGGCTCGTACAAGTTTAGCAGCGACAGGACCATTCACGAATATGCTAAGGACATATGGAACATCAACCCCGTCGAGCTGCATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0001101 GO:0003674 GO:0003824 GO:0004645 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005977 GO:0005980 GO:0006073 GO:0006091 GO:0006112 GO:0006950 GO:0008144 GO:0008150 GO:0008152 GO:0008184 GO:0009056 GO:0009057 GO:0009251 GO:0009266 GO:0009414 GO:0009415 GO:0009501 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009987 GO:0010035 GO:0015980 GO:0016052 GO:0016740 GO:0016757 GO:0016758 GO:0019842 GO:0030170 GO:0036094 GO:0042221 GO:0042802 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048037 GO:0050662 GO:0050896 GO:0055114 GO:0070279 GO:0071704 GO:0097159 GO:1901363 GO:1901575 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 ko02026 ko04217 ko04910 ko04922 ko04931 map00500 map01100 map01110 map02026 map04217 map04910 map04922 map04931

Pfam Domains

Protein Families

Phosphorylase

Protein Analysis

1006

Amino Acids

113.66

Weight (kDa)

5.31

Isoelectric Point (pI)

43.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Phosphorylase	PF00343	172 - 494	9.3e-129	Carbohydrate phosphorylase
Phosphorylase	PF00343	584 - 999	1.4e-167	Carbohydrate phosphorylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002988)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G29320
fragaria_vesca	FvH4_4g30370 FvH4_6g47260 FvH4_6g47260 FvH4_6g47260 FvH4_6g47260
malus_domestica	MD00G1156200.v1.1 MD09G1062600.v1.1 MD16G1063300.v1.1
prunus_persica	Prupe.1G283400_v2.0.a1 Prupe.1G283400_v2.0.a1 Prupe.3G257600_v2.0.a1
pyrus_communis	pycom111g05290
rosa_chinensis	RchiOBHm_Chr2g0166331 RchiOBHm_Chr4g0438671
rosa_laevigata	RLG00000006342 RLG00000021610
rosa_multiflora	Rmu_sc0000738.1_g000042 Rmu_sc0002505.1_g000002 Rmu_sc0007974.1_g000007
rosa_roxburghii	Rroxscaffold_2G00084990 Rroxscaffold_5G00379690 Rroxscaffold_7G00200130
rosa_rugosa	Rorug02G0522200 Rorug04G0312500 Rorug04G0312600 Rorug04G0312600
rosa_samantha	Rh2AG588200 Rh2BG600200 Rh2CG571100 Rh2DG610400 Rh4AG363000 Rh4BG374300 Rh4CG388400 Rh4DG368900
rosa_wichuraiana	Rw0G007140 Rw0G016690 Rw2G049020

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1914, 2006
AasI	GACNNNNNNGTC	1 cut(s)	2914
AccB1I	GGYRCC	1 cut(s)	2594
AccB7I	CCANNNNNTGG	1 cut(s)	872
AccII	CGCG	1 cut(s)	1764
AccIII	TCCGGA	2 cut(s)	304, 2707
AciI	CCGC	7 cut(s)	33, 503, 904, 1975, 2257, 2517, 2748
AclI	AACGTT	1 cut(s)	623
AclWI	GGATC	7 cut(s)	1966, 1979, 2094, 2405, 2781, 2794, 2914
AcoI	YGGCCR	3 cut(s)	140, 2055, 2812
AcsI	RAATTY	9 cut(s)	224, 284, 440, 779, 1221, 1499, 1931, 2375, 2735
AcuI	CTGAAG	3 cut(s)	1002, 2271, 2453
AcvI	CACGTG	1 cut(s)	2315
AdeI	CACNNNGTG	1 cut(s)	350
AfaI	GTAC	8 cut(s)	240, 659, 1017, 1749, 1815, 2515, 2765, 2942
AfeI	AGCGCT	1 cut(s)	459
AfiI	CCNNNNNNNGG	6 cut(s)	641, 872, 1162, 2103, 2104, 2420
AflII	CTTAAG	1 cut(s)	1457
AflIII	ACRYGT	2 cut(s)	1363, 2314
AjnI	CCWGG	8 cut(s)	89, 549, 740, 871, 1024, 1259, 2790, 2814
AjuI	GAANNNNNNNTTGG	4 cut(s)	98, 130, 805, 837
AloI	GAACNNNNNNTCC	2 cut(s)	2688, 2720
Alw21I	GWGCWC	4 cut(s)	189, 969, 1398, 2499
Alw26I	GTCTC	3 cut(s)	35, 769, 2886
AlwI	GGATC	7 cut(s)	1966, 1979, 2094, 2405, 2781, 2794, 2914
AlwNI	CAGNNNCTG	1 cut(s)	563
Ama87I	CYCGRG	2 cut(s)	1157, 1546
Aor13HI	TCCGGA	2 cut(s)	304, 2707
Aor51HI	AGCGCT	1 cut(s)	459
AoxI	GGCC	7 cut(s)	140, 327, 1257, 1314, 1922, 2055, 2812
ApeKI	GCWGC	9 cut(s)	5, 30, 215, 248, 563, 1351, 2568, 2952, 3010
ApoI	RAATTY	9 cut(s)	224, 284, 440, 779, 1221, 1499, 1931, 2375, 2735
ArsI	GACNNNNNNTTYG	6 cut(s)	1042, 1074, 1396, 1428, 2702, 2734
AseI	ATTAAT	1 cut(s)	2403
AspLEI	GCGC	3 cut(s)	364, 460, 2208
AspS9I	GGNCC	2 cut(s)	2710, 2961
AsuHPI	GGTGA	5 cut(s)	280, 1736, 1966, 2212, 2435
AvaI	CYCGRG	2 cut(s)	1157, 1546
AvaII	GGWCC	2 cut(s)	2710, 2961
AxyI	CCTNAGG	1 cut(s)	1311
BaeI	ACNNNNGTAYC	2 cut(s)	2497, 2530
BalI	TGGCCA	1 cut(s)	2814
BamHI	GGATCC	2 cut(s)	1971, 2786
BanI	GGYRCC	1 cut(s)	2594
BanII	GRGCYC	3 cut(s)	189, 1398, 2499
BarI	GAAGNNNNNNTAC	2 cut(s)	1731, 1763
BauI	CACGAG	1 cut(s)	182
BbrPI	CACGTG	1 cut(s)	2315
BbsI	GAAGAC	4 cut(s)	843, 2034, 2434, 2634
Bbv12I	GWGCWC	4 cut(s)	189, 969, 1398, 2499
BbvCI	CCTCAGC	2 cut(s)	714, 2691
BbvI	GCAGC	9 cut(s)	17, 42, 202, 235, 550, 1338, 2555, 2964, 2997
BccI	CCATC	7 cut(s)	263, 485, 689, 724, 803, 1962, 2898
BceAI	ACGGC	1 cut(s)	127
BcgI	CGANNNNNNTGC	2 cut(s)	194, 228
BciT130I	CCWGG	8 cut(s)	91, 551, 742, 873, 1026, 1261, 2792, 2816
BciVI	GTATCC	1 cut(s)	869
BclI	TGATCA	1 cut(s)	2145
BcoDI	GTCTC	3 cut(s)	35, 769, 2886
BfaI	CTAG	2 cut(s)	2415, 2588
BfmI	CTRYAG	1 cut(s)	446
BfoI	RGCGCY	1 cut(s)	461
BfrI	CTTAAG	1 cut(s)	1457
BfuI	GTATCC	1 cut(s)	869
BglII	AGATCT	1 cut(s)	1124
Bme1390I	CCNGG	8 cut(s)	91, 551, 742, 873, 1026, 1261, 2792, 2816
Bme18I	GGWCC	2 cut(s)	2710, 2961
BmeT110I	CYCGRG	2 cut(s)	1157, 1546
BmgT120I	GGNCC	2 cut(s)	2710, 2961
BmiI	GGNNCC	8 cut(s)	88, 555, 1797, 1973, 2539, 2596, 2712, 2788
BmrFI	CCNGG	8 cut(s)	91, 551, 742, 873, 1026, 1261, 2792, 2816
BmrI	ACTGGG	2 cut(s)	2041, 2844
BmuI	ACTGGG	2 cut(s)	2041, 2844
BpiI	GAAGAC	4 cut(s)	843, 2034, 2434, 2634
BplI	GAGNNNNNCTC	2 cut(s)	1255, 1287
BpmI	CTGGAG	3 cut(s)	981, 1149, 1282
Bpu10I	CCTNAGC	3 cut(s)	714, 2691, 2979
BpuEI	CTTGAG	2 cut(s)	173, 746
Bsa29I	ATCGAT	1 cut(s)	78
BsaAI	YACGTR	4 cut(s)	1015, 1817, 2315, 2349
BsaBI	GATNNNNATC	1 cut(s)	1188
BsaI	GGTCTC	1 cut(s)	2886
BsaJI	CCNNGG	5 cut(s)	740, 741, 1025, 1317, 2790
BsaWI	WCCGGW	2 cut(s)	304, 2707
BsaXI	ACNNNNNCTCC	2 cut(s)	1122, 1152
Bsc4I	CCNNNNNNNGG	6 cut(s)	641, 872, 1162, 2103, 2104, 2420
Bse1I	ACTGG	7 cut(s)	380, 805, 1253, 2036, 2058, 2532, 2850
Bse21I	CCTNAGG	1 cut(s)	1311
Bse3DI	GCAATG	3 cut(s)	52, 2190, 2565
Bse8I	GATNNNNATC	1 cut(s)	1188
BseAI	TCCGGA	2 cut(s)	304, 2707
BseBI	CCWGG	8 cut(s)	91, 551, 742, 873, 1026, 1261, 2792, 2816
BseCI	ATCGAT	1 cut(s)	78
BseDI	CCNNGG	5 cut(s)	740, 741, 1025, 1317, 2790
BseGI	GGATG	4 cut(s)	814, 1036, 1189, 2571
BseJI	GATNNNNATC	1 cut(s)	1188
BseLI	CCNNNNNNNGG	6 cut(s)	641, 872, 1162, 2103, 2104, 2420
BseMI	GCAATG	3 cut(s)	52, 2190, 2565
BseMII	CTCAG	5 cut(s)	705, 1302, 1682, 2666, 2682
BseNI	ACTGG	7 cut(s)	380, 805, 1253, 2036, 2058, 2532, 2850
BseRI	GAGGAG	2 cut(s)	846, 1406
BseX3I	CGGCCG	1 cut(s)	140
BseXI	GCAGC	9 cut(s)	17, 42, 202, 235, 550, 1338, 2555, 2964, 2997
BseYI	CCCAGC	1 cut(s)	2669
BsgI	GTGCAG	3 cut(s)	327, 549, 1193
Bsh1236I	CGCG	1 cut(s)	1764
Bsh1285I	CGRYCG	1 cut(s)	143
BshFI	GGCC	7 cut(s)	142, 329, 1259, 1316, 1924, 2057, 2814
BshNI	GGYRCC	1 cut(s)	2594
BshVI	ATCGAT	1 cut(s)	78
BsiEI	CGRYCG	1 cut(s)	143
BsiHKAI	GWGCWC	4 cut(s)	189, 969, 1398, 2499
BsiHKCI	CYCGRG	2 cut(s)	1157, 1546
BsiSI	CCGG	2 cut(s)	305, 2708
BslFI	GGGAC	3 cut(s)	1529, 2432, 2596
BslI	CCNNNNNNNGG	6 cut(s)	641, 872, 1162, 2103, 2104, 2420
BsmAI	GTCTC	3 cut(s)	35, 769, 2886
BsmFI	GGGAC	3 cut(s)	1529, 2432, 2596
BsmI	GAATGC	3 cut(s)	385, 1203, 2208
BsnI	GGCC	7 cut(s)	142, 329, 1259, 1316, 1924, 2057, 2814
Bso31I	GGTCTC	1 cut(s)	2886
BsoBI	CYCGRG	2 cut(s)	1157, 1546
Bsp1286I	GDGCHC	4 cut(s)	189, 969, 1398, 2499
Bsp13I	TCCGGA	2 cut(s)	304, 2707
BspACI	CCGC	7 cut(s)	33, 503, 904, 1975, 2257, 2517, 2748
BspANI	GGCC	7 cut(s)	142, 329, 1259, 1316, 1924, 2057, 2814
BspCNI	CTCAG	5 cut(s)	706, 1303, 1683, 2667, 2683
BspDI	ATCGAT	1 cut(s)	78
BspEI	TCCGGA	2 cut(s)	304, 2707
BspFNI	CGCG	1 cut(s)	1764
BspHI	TCATGA	3 cut(s)	2212, 2407, 2659
BspLI	GGNNCC	8 cut(s)	88, 555, 1797, 1973, 2539, 2596, 2712, 2788
BspPI	GGATC	7 cut(s)	1966, 1979, 2094, 2405, 2781, 2794, 2914
BspT107I	GGYRCC	1 cut(s)	2594
BspTI	CTTAAG	1 cut(s)	1457
BspTNI	GGTCTC	1 cut(s)	2886
BsrDI	GCAATG	3 cut(s)	52, 2190, 2565
BsrI	ACTGG	7 cut(s)	380, 805, 1253, 2036, 2058, 2532, 2850
BssECI	CCNNGG	5 cut(s)	740, 741, 1025, 1317, 2790
BssSI	CACGAG	1 cut(s)	182
BssT1I	CCWWGG	1 cut(s)	1317
Bst2BI	CACGAG	1 cut(s)	182
Bst2UI	CCWGG	8 cut(s)	91, 551, 742, 873, 1026, 1261, 2792, 2816
Bst4CI	ACNGT	5 cut(s)	1282, 1303, 1877, 2478, 2594
Bst6I	CTCTTC	8 cut(s)	1143, 1566, 1629, 1638, 1701, 1710, 1761, 2610
BstAFI	CTTAAG	1 cut(s)	1457
BstBAI	YACGTR	4 cut(s)	1015, 1817, 2315, 2349
BstC8I	GCNNGC	7 cut(s)	220, 420, 518, 594, 1494, 2358, 2935
BstDEI	CTNAG	6 cut(s)	714, 1311, 1691, 2675, 2691, 2979
BstF5I	GGATG	4 cut(s)	814, 1036, 1189, 2571
BstFNI	CGCG	1 cut(s)	1764
BstH2I	RGCGCY	1 cut(s)	461
BstHHI	GCGC	3 cut(s)	364, 460, 2208
BstMAI	GTCTC	3 cut(s)	35, 769, 2886
BstMCI	CGRYCG	1 cut(s)	143
BstMWI	GCNNNNNNNGC	8 cut(s)	44, 184, 228, 257, 335, 419, 983, 2503
BstNI	CCWGG	8 cut(s)	91, 551, 742, 873, 1026, 1261, 2792, 2816
BstNSI	RCATGY	1 cut(s)	1367
BstSCI	CCNGG	8 cut(s)	89, 549, 740, 871, 1024, 1259, 2790, 2814
BstSFI	CTRYAG	1 cut(s)	446
BstSNI	TACGTA	3 cut(s)	1015, 1817, 2349
BstUI	CGCG	1 cut(s)	1764
BstV1I	GCAGC	9 cut(s)	17, 42, 202, 235, 550, 1338, 2555, 2964, 2997
BstV2I	GAAGAC	4 cut(s)	843, 2034, 2434, 2634
BstX2I	RGATCY	4 cut(s)	1124, 1971, 2086, 2786
BstXI	CCANNNNNNTGG	1 cut(s)	1968
BstYI	RGATCY	4 cut(s)	1124, 1971, 2086, 2786
BstZI	CGGCCG	1 cut(s)	140
Bsu15I	ATCGAT	1 cut(s)	78
Bsu36I	CCTNAGG	1 cut(s)	1311
BsuI	GTATCC	1 cut(s)	869
BsuRI	GGCC	7 cut(s)	142, 329, 1259, 1316, 1924, 2057, 2814
BsuTUI	ATCGAT	1 cut(s)	78
BtgZI	GCGATG	1 cut(s)	241
BtsCI	GGATG	4 cut(s)	814, 1036, 1189, 2571
BtsI	GCAGTG	4 cut(s)	1176, 1536, 1608, 1680
BtsIMutI	CAGTG	7 cut(s)	1176, 1536, 1608, 1680, 1882, 2470, 2539
Cac8I	GCNNGC	7 cut(s)	220, 420, 518, 594, 1494, 2358, 2935
CaiI	CAGNNNCTG	1 cut(s)	563
CciI	TCATGA	3 cut(s)	2212, 2407, 2659
CfoI	GCGC	3 cut(s)	364, 460, 2208
Cfr13I	GGNCC	2 cut(s)	2710, 2961
ClaI	ATCGAT	1 cut(s)	78
Csp6I	GTAC	8 cut(s)	239, 658, 1016, 1748, 1814, 2514, 2764, 2941
CviAII	CATG	6 cut(s)	639, 1364, 2213, 2408, 2548, 2660
CviQI	GTAC	8 cut(s)	239, 658, 1016, 1748, 1814, 2514, 2764, 2941
DdeI	CTNAG	6 cut(s)	714, 1311, 1691, 2675, 2691, 2979
DraI	TTTAAA	1 cut(s)	676
DraIII	CACNNNGTG	1 cut(s)	350
DrdI	GACNNNNNNGTC	1 cut(s)	2914
DseDI	GACNNNNNNGTC	1 cut(s)	2914
EaeI	YGGCCR	3 cut(s)	140, 2055, 2812
EagI	CGGCCG	1 cut(s)	140
Eam1104I	CTCTTC	8 cut(s)	1143, 1566, 1629, 1638, 1701, 1710, 1761, 2610
EarI	CTCTTC	8 cut(s)	1143, 1566, 1629, 1638, 1701, 1710, 1761, 2610
Ecl136II	GAGCTC	3 cut(s)	187, 1396, 2497
EclXI	CGGCCG	1 cut(s)	140
Eco105I	TACGTA	3 cut(s)	1015, 1817, 2349
Eco130I	CCWWGG	1 cut(s)	1317
Eco147I	AGGCCT	3 cut(s)	329, 1259, 1316
Eco24I	GRGCYC	3 cut(s)	189, 1398, 2499
Eco31I	GGTCTC	1 cut(s)	2886
Eco47I	GGWCC	2 cut(s)	2710, 2961
Eco47III	AGCGCT	1 cut(s)	459
Eco52I	CGGCCG	1 cut(s)	140
Eco53kI	GAGCTC	3 cut(s)	187, 1396, 2497
Eco57I	CTGAAG	3 cut(s)	1002, 2271, 2453
Eco72I	CACGTG	1 cut(s)	2315
Eco81I	CCTNAGG	1 cut(s)	1311
Eco88I	CYCGRG	2 cut(s)	1157, 1546
EcoICRI	GAGCTC	3 cut(s)	187, 1396, 2497
EcoRI	GAATTC	2 cut(s)	224, 284
EcoRII	CCWGG	8 cut(s)	89, 549, 740, 871, 1024, 1259, 2790, 2814
EcoT14I	CCWWGG	1 cut(s)	1317
EcoT38I	GRGCYC	3 cut(s)	189, 1398, 2499
ErhI	CCWWGG	1 cut(s)	1317
FaeI	CATG	6 cut(s)	642, 1367, 2216, 2411, 2551, 2663
FalI	AAGNNNNNCTT	6 cut(s)	317, 349, 653, 685, 2426, 2458
FaqI	GGGAC	3 cut(s)	1529, 2432, 2596
FatI	CATG	6 cut(s)	638, 1363, 2212, 2407, 2547, 2659
FauNDI	CATATG	2 cut(s)	499, 2987
FbaI	TGATCA	1 cut(s)	2145
FokI	GGATG	4 cut(s)	821, 1043, 1176, 2558
FriOI	GRGCYC	3 cut(s)	189, 1398, 2499
FspBI	CTAG	2 cut(s)	2415, 2588
GlaI	GCGC	3 cut(s)	363, 459, 2207
GsaI	CCCAGC	1 cut(s)	2673
GsuI	CTGGAG	3 cut(s)	981, 1149, 1282
HaeII	RGCGCY	1 cut(s)	461
HaeIII	GGCC	7 cut(s)	142, 329, 1259, 1316, 1924, 2057, 2814
HapII	CCGG	2 cut(s)	305, 2708
HhaI	GCGC	3 cut(s)	364, 460, 2208
Hin1II	CATG	6 cut(s)	642, 1367, 2216, 2411, 2551, 2663
Hin6I	GCGC	3 cut(s)	362, 458, 2206
HinP1I	GCGC	3 cut(s)	362, 458, 2206
HincII	GTYRAC	4 cut(s)	373, 915, 1486, 2173
HindII	GTYRAC	4 cut(s)	373, 915, 1486, 2173
HindIII	AAGCTT	5 cut(s)	107, 507, 518, 921, 984
HpaI	GTTAAC	1 cut(s)	373
HpaII	CCGG	2 cut(s)	305, 2708
HphI	GGTGA	5 cut(s)	280, 1736, 1966, 2212, 2435
Hpy166II	GTNNAC	6 cut(s)	117, 373, 915, 1486, 2173, 2254
Hpy8I	GTNNAC	6 cut(s)	117, 373, 915, 1486, 2173, 2254
Hpy99I	CGWCG	1 cut(s)	3008
HpyCH4III	ACNGT	5 cut(s)	1282, 1303, 1877, 2478, 2594
HpyCH4IV	ACGT	7 cut(s)	623, 1014, 1111, 1816, 2314, 2348, 2716
HpyF10VI	GCNNNNNNNGC	8 cut(s)	44, 184, 228, 257, 335, 419, 983, 2503
HpyF3I	CTNAG	6 cut(s)	714, 1311, 1691, 2675, 2691, 2979
HpySE526I	ACGT	7 cut(s)	623, 1014, 1111, 1816, 2314, 2348, 2716
Hsp92II	CATG	6 cut(s)	642, 1367, 2216, 2411, 2551, 2663
HspAI	GCGC	3 cut(s)	362, 458, 2206
Kpn2I	TCCGGA	2 cut(s)	304, 2707
Ksp22I	TGATCA	1 cut(s)	2145
KspAI	GTTAAC	1 cut(s)	373
LmnI	GCTCC	4 cut(s)	35, 553, 1130, 1393
Lsp1109I	GCAGC	9 cut(s)	17, 42, 202, 235, 550, 1338, 2555, 2964, 2997
MaeI	CTAG	2 cut(s)	2415, 2588
MaeII	ACGT	7 cut(s)	623, 1014, 1111, 1816, 2314, 2348, 2716
MaeIII	GTNAC	5 cut(s)	796, 1844, 1954, 1997, 2852
MflI	RGATCY	4 cut(s)	1124, 1971, 2086, 2786
MhlI	GDGCHC	4 cut(s)	189, 969, 1398, 2499
MlsI	TGGCCA	1 cut(s)	2814
MluNI	TGGCCA	1 cut(s)	2814
MlyI	GAGTC	6 cut(s)	130, 605, 1339, 1646, 1718, 2578
MmeI	TCCRAC	1 cut(s)	1217
Mox20I	TGGCCA	1 cut(s)	2814
MroI	TCCGGA	2 cut(s)	304, 2707
MscI	TGGCCA	1 cut(s)	2814
MslI	CAYNNNNRTG	2 cut(s)	1379, 2973
Msp20I	TGGCCA	1 cut(s)	2814
MspA1I	CMGCKG	3 cut(s)	33, 682, 2519
MspCI	CTTAAG	1 cut(s)	1457
MspI	CCGG	2 cut(s)	305, 2708
MspR9I	CCNGG	8 cut(s)	91, 551, 742, 873, 1026, 1261, 2792, 2816
Mva1269I	GAATGC	3 cut(s)	385, 1203, 2208
MvaI	CCWGG	8 cut(s)	91, 551, 742, 873, 1026, 1261, 2792, 2816
MvnI	CGCG	1 cut(s)	1764
MwoI	GCNNNNNNNGC	8 cut(s)	44, 184, 228, 257, 335, 419, 983, 2503
NdeI	CATATG	2 cut(s)	499, 2987
NlaIII	CATG	6 cut(s)	642, 1367, 2216, 2411, 2551, 2663
NlaIV	GGNNCC	8 cut(s)	88, 555, 1797, 1973, 2539, 2596, 2712, 2788
NmeAIII	GCCGAG	1 cut(s)	118
NmuCI	GTSAC	2 cut(s)	1844, 1954
NspI	RCATGY	1 cut(s)	1367
PagI	TCATGA	3 cut(s)	2212, 2407, 2659
PasI	CCCWGGG	1 cut(s)	741
PceI	AGGCCT	3 cut(s)	329, 1259, 1316
PciI	ACATGT	1 cut(s)	1363
PctI	GAATGC	3 cut(s)	385, 1203, 2208
PfeI	GAWTC	7 cut(s)	130, 193, 1034, 1583, 1655, 1727, 1825
PflFI	GACNNNGTC	1 cut(s)	2033
PflMI	CCANNNNNTGG	1 cut(s)	872
PfoI	TCCNGGA	1 cut(s)	89
PleI	GAGTC	6 cut(s)	130, 605, 1338, 1645, 1717, 2578
PmaCI	CACGTG	1 cut(s)	2315
PmlI	CACGTG	1 cut(s)	2315
PpsI	GAGTC	6 cut(s)	130, 605, 1338, 1645, 1717, 2578
Ppu21I	YACGTR	4 cut(s)	1015, 1817, 2315, 2349
PscI	ACATGT	1 cut(s)	1363
PshBI	ATTAAT	1 cut(s)	2403
PsiI	TTATAA	2 cut(s)	1914, 2006
Psp124BI	GAGCTC	3 cut(s)	189, 1398, 2499
Psp1406I	AACGTT	1 cut(s)	623
Psp6I	CCWGG	8 cut(s)	89, 549, 740, 871, 1024, 1259, 2790, 2814
PspCI	CACGTG	1 cut(s)	2315
PspFI	CCCAGC	1 cut(s)	2669
PspGI	CCWGG	8 cut(s)	89, 549, 740, 871, 1024, 1259, 2790, 2814
PspN4I	GGNNCC	8 cut(s)	88, 555, 1797, 1973, 2539, 2596, 2712, 2788
PspPI	GGNCC	2 cut(s)	2710, 2961
PsrI	GAACNNNNNNTAC	2 cut(s)	150, 182
PstNI	CAGNNNCTG	1 cut(s)	563
PsuI	RGATCY	4 cut(s)	1124, 1971, 2086, 2786
PsyI	GACNNNGTC	1 cut(s)	2033
PvuII	CAGCTG	1 cut(s)	682
RsaI	GTAC	8 cut(s)	240, 659, 1017, 1749, 1815, 2515, 2765, 2942
RsaNI	GTAC	8 cut(s)	239, 658, 1016, 1748, 1814, 2514, 2764, 2941
RseI	CAYNNNNRTG	2 cut(s)	1379, 2973
SacI	GAGCTC	3 cut(s)	189, 1398, 2499
Sau96I	GGNCC	2 cut(s)	2710, 2961
SchI	GAGTC	6 cut(s)	130, 605, 1339, 1646, 1718, 2578
ScrFI	CCNGG	8 cut(s)	91, 551, 742, 873, 1026, 1261, 2792, 2816
SduI	GDGCHC	4 cut(s)	189, 969, 1398, 2499
SfcI	CTRYAG	1 cut(s)	446
SinI	GGWCC	2 cut(s)	2710, 2961
SmiMI	CAYNNNNRTG	2 cut(s)	1379, 2973
SmlI	CTYRAG	3 cut(s)	188, 725, 1457
SmoI	CTYRAG	3 cut(s)	188, 725, 1457
SnaBI	TACGTA	3 cut(s)	1015, 1817, 2349
SseBI	AGGCCT	3 cut(s)	329, 1259, 1316
SsiI	CCGC	7 cut(s)	33, 503, 904, 1975, 2257, 2517, 2748
SspI	AATATT	2 cut(s)	1473, 2239
SspMI	CTAG	2 cut(s)	2415, 2588
SstI	GAGCTC	3 cut(s)	189, 1398, 2499
StuI	AGGCCT	3 cut(s)	329, 1259, 1316
StyD4I	CCNGG	8 cut(s)	89, 549, 740, 871, 1024, 1259, 2790, 2814
StyI	CCWWGG	1 cut(s)	1317
TaaI	ACNGT	5 cut(s)	1282, 1303, 1877, 2478, 2594
TaiI	ACGT	7 cut(s)	626, 1017, 1114, 1819, 2317, 2351, 2719
TaqI	TCGA	8 cut(s)	78, 204, 263, 1360, 2877, 2890, 2918, 3006
TauI	GCSGC	1 cut(s)	907
TfiI	GAWTC	7 cut(s)	130, 193, 1034, 1583, 1655, 1727, 1825
TscAI	CASTG	7 cut(s)	1176, 1543, 1615, 1687, 1882, 2470, 2539
TseFI	GTSAC	2 cut(s)	1844, 1954
TseI	GCWGC	9 cut(s)	5, 30, 215, 248, 563, 1351, 2568, 2952, 3010
Tsp45I	GTSAC	2 cut(s)	1844, 1954
TspGWI	ACGGA	2 cut(s)	591, 2039
TspRI	CASTG	7 cut(s)	1176, 1543, 1615, 1687, 1882, 2470, 2539
Tth111I	GACNNNGTC	1 cut(s)	2033
Van91I	CCANNNNNTGG	1 cut(s)	872
Vha464I	CTTAAG	1 cut(s)	1457
VpaK11BI	GGWCC	2 cut(s)	2710, 2961
VspI	ATTAAT	1 cut(s)	2403
XapI	RAATTY	9 cut(s)	224, 284, 440, 779, 1221, 1499, 1931, 2375, 2735
XbaI	TCTAGA	1 cut(s)	2587
XceI	RCATGY	1 cut(s)	1367
XspI	CTAG	2 cut(s)	2415, 2588

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.