Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT3G29320 |
| fragaria_vesca | FvH4_4g30370 FvH4_6g47260 FvH4_6g47260 FvH4_6g47260 FvH4_6g47260 |
| malus_domestica | MD00G1156200.v1.1 MD09G1062600.v1.1 MD16G1063300.v1.1 |
| prunus_persica | Prupe.1G283400_v2.0.a1 Prupe.1G283400_v2.0.a1 Prupe.3G257600_v2.0.a1 |
| pyrus_communis | pycom111g05290 |
| rosa_chinensis | RchiOBHm_Chr2g0166331 RchiOBHm_Chr4g0438671 |
| rosa_laevigata | RLG00000006342 RLG00000021610 |
| rosa_multiflora | Rmu_sc0000738.1_g000042 Rmu_sc0002505.1_g000002 Rmu_sc0007974.1_g000007 |
| rosa_roxburghii | Rroxscaffold_2G00084990 Rroxscaffold_5G00379690 Rroxscaffold_7G00200130 |
| rosa_rugosa | Rorug02G0522200 Rorug04G0312500 Rorug04G0312600 Rorug04G0312600 |
| rosa_samantha | Rh2AG588200 Rh2BG600200 Rh2CG571100 Rh2DG610400 Rh4AG363000 Rh4BG374300 Rh4CG388400 Rh4DG368900 |
| rosa_wichuraiana | Rw0G007140 Rw0G016690 Rw2G049020 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1469 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 2377 |
| Acc16I | TGCGCA | 1 cut(s) | 1280 |
| AccI | GTMKAC | 2 cut(s) | 1462, 2035 |
| AccII | CGCG | 2 cut(s) | 1808, 1872 |
| AciI | CCGC | 3 cut(s) | 453, 1870, 2171 |
| AclWI | GGATC | 6 cut(s) | 1047, 1250, 2168, 2181, 2257, 2465 |
| AcoI | YGGCCR | 1 cut(s) | 304 |
| AcuI | CTGAAG | 2 cut(s) | 2142, 2175 |
| AfaI | GTAC | 6 cut(s) | 629, 1215, 1659, 1978, 2425, 2472 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 247, 248, 479 |
| AflIII | ACRYGT | 1 cut(s) | 970 |
| AjnI | CCWGG | 5 cut(s) | 222, 306, 478, 866, 1948 |
| Alw26I | GTCTC | 3 cut(s) | 435, 1194, 2439 |
| AlwI | GGATC | 6 cut(s) | 1047, 1250, 2168, 2181, 2257, 2465 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 170, 1259 |
| Ama87I | CYCGRG | 2 cut(s) | 632, 917 |
| AoxI | GGCC | 5 cut(s) | 101, 304, 410, 1385, 1992 |
| ApeKI | GCWGC | 5 cut(s) | 170, 530, 590, 1264, 1690 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 759, 1802 |
| AspLEI | GCGC | 2 cut(s) | 1281, 1671 |
| AspS9I | GGNCC | 5 cut(s) | 101, 410, 519, 739, 2222 |
| AsuC2I | CCSGG | 3 cut(s) | 242, 633, 634 |
| AsuHPI | GGTGA | 5 cut(s) | 407, 442, 1531, 1675, 1898 |
| AvaI | CYCGRG | 2 cut(s) | 632, 917 |
| AvaII | GGWCC | 3 cut(s) | 519, 739, 2222 |
| BaeGI | GKGCMC | 1 cut(s) | 135 |
| BalI | TGGCCA | 1 cut(s) | 306 |
| BamHI | GGATCC | 1 cut(s) | 2173 |
| BbvI | GCAGC | 5 cut(s) | 157, 517, 602, 1251, 1702 |
| BccI | CCATC | 8 cut(s) | 296, 331, 410, 1267, 1425, 2048, 2239, 2361 |
| BceAI | ACGGC | 1 cut(s) | 1703 |
| BciT130I | CCWGG | 5 cut(s) | 224, 308, 480, 868, 1950 |
| BciVI | GTATCC | 1 cut(s) | 2294 |
| BclI | TGATCA | 1 cut(s) | 1150 |
| BcnI | CCSGG | 3 cut(s) | 242, 633, 634 |
| BcoDI | GTCTC | 3 cut(s) | 435, 1194, 2439 |
| BfaI | CTAG | 8 cut(s) | 153, 191, 1082, 1631, 2004, 2051, 2115, 2313 |
| BfmI | CTRYAG | 1 cut(s) | 2466 |
| BfuI | GTATCC | 1 cut(s) | 2294 |
| BglII | AGATCT | 3 cut(s) | 612, 1453, 2150 |
| BisI | GCNGC | 5 cut(s) | 171, 531, 591, 1265, 1691 |
| BlsI | GCNGC | 5 cut(s) | 172, 532, 592, 1266, 1692 |
| BmcAI | AGTACT | 3 cut(s) | 1215, 1978, 2472 |
| Bme1390I | CCNGG | 8 cut(s) | 224, 242, 308, 480, 633, 634, 868, 1950 |
| Bme18I | GGWCC | 3 cut(s) | 519, 739, 2222 |
| BmeT110I | CYCGRG | 2 cut(s) | 632, 917 |
| BmgT120I | GGNCC | 5 cut(s) | 101, 410, 519, 739, 2222 |
| BmiI | GGNNCC | 6 cut(s) | 162, 411, 740, 741, 1858, 2175 |
| BmrFI | CCNGG | 8 cut(s) | 224, 242, 308, 480, 633, 634, 868, 1950 |
| BmsI | GCATC | 3 cut(s) | 157, 1610, 1680 |
| BoxI | GACNNNNGTC | 1 cut(s) | 517 |
| BpmI | CTGGAG | 1 cut(s) | 588 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1260 |
| BpuEI | CTTGAG | 4 cut(s) | 161, 353, 1194, 1595 |
| BpuMI | CCSGG | 3 cut(s) | 242, 633, 634 |
| Bsa29I | ATCGAT | 1 cut(s) | 1720 |
| BsaBI | GATNNNNATC | 1 cut(s) | 795 |
| BsaI | GGTCTC | 1 cut(s) | 1194 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 432, 462, 1848, 1878 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 247, 248, 479 |
| Bse1I | ACTGG | 5 cut(s) | 571, 1221, 1555, 1627, 1995 |
| Bse3DI | GCAATG | 1 cut(s) | 2028 |
| Bse8I | GATNNNNATC | 1 cut(s) | 795 |
| BseBI | CCWGG | 5 cut(s) | 224, 308, 480, 868, 1950 |
| BseCI | ATCGAT | 1 cut(s) | 1720 |
| BseGI | GGATG | 5 cut(s) | 151, 643, 796, 844, 1671 |
| BseJI | GATNNNNATC | 1 cut(s) | 795 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 247, 248, 479 |
| BseMI | GCAATG | 1 cut(s) | 2028 |
| BseMII | CTCAG | 6 cut(s) | 1014, 1215, 1251, 1320, 1380, 1500 |
| BseNI | ACTGG | 5 cut(s) | 571, 1221, 1555, 1627, 1995 |
| BseRI | GAGGAG | 3 cut(s) | 453, 1013, 2221 |
| BseSI | GKGCMC | 1 cut(s) | 135 |
| BseXI | GCAGC | 5 cut(s) | 157, 517, 602, 1251, 1702 |
| BseYI | CCCAGC | 1 cut(s) | 1965 |
| BsgI | GTGCAG | 2 cut(s) | 516, 800 |
| Bsh1236I | CGCG | 2 cut(s) | 1808, 1872 |
| Bsh1285I | CGRYCG | 1 cut(s) | 2278 |
| BshFI | GGCC | 5 cut(s) | 103, 306, 412, 1387, 1994 |
| BshVI | ATCGAT | 1 cut(s) | 1720 |
| BsiEI | CGRYCG | 1 cut(s) | 2278 |
| BsiHKCI | CYCGRG | 2 cut(s) | 632, 917 |
| BsiSI | CCGG | 2 cut(s) | 242, 633 |
| BslFI | GGGAC | 4 cut(s) | 455, 752, 1731, 1948 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 247, 248, 479 |
| BsmAI | GTCTC | 3 cut(s) | 435, 1194, 2439 |
| BsmFI | GGGAC | 4 cut(s) | 455, 752, 1731, 1948 |
| BsmI | GAATGC | 4 cut(s) | 607, 810, 1802, 2330 |
| BsnI | GGCC | 5 cut(s) | 103, 306, 412, 1387, 1994 |
| Bso31I | GGTCTC | 1 cut(s) | 1194 |
| BsoBI | CYCGRG | 2 cut(s) | 632, 917 |
| Bsp1286I | GDGCHC | 1 cut(s) | 135 |
| Bsp1407I | TGTACA | 1 cut(s) | 1657 |
| Bsp19I | CCATGG | 3 cut(s) | 347, 645, 1143 |
| Bsp68I | TCGCGA | 1 cut(s) | 1808 |
| BspACI | CCGC | 3 cut(s) | 453, 1870, 2171 |
| BspANI | GGCC | 5 cut(s) | 103, 306, 412, 1387, 1994 |
| BspCNI | CTCAG | 6 cut(s) | 1015, 1216, 1252, 1321, 1381, 1501 |
| BspDI | ATCGAT | 1 cut(s) | 1720 |
| BspFNI | CGCG | 2 cut(s) | 1808, 1872 |
| BspHI | TCATGA | 1 cut(s) | 2431 |
| BspLI | GGNNCC | 6 cut(s) | 162, 411, 740, 741, 1858, 2175 |
| BspPI | GGATC | 6 cut(s) | 1047, 1250, 2168, 2181, 2257, 2465 |
| BspTNI | GGTCTC | 1 cut(s) | 1194 |
| BsrDI | GCAATG | 1 cut(s) | 2028 |
| BsrGI | TGTACA | 1 cut(s) | 1657 |
| BsrI | ACTGG | 5 cut(s) | 571, 1221, 1555, 1627, 1995 |
| BssNAI | GTATAC | 1 cut(s) | 2036 |
| BssT1I | CCWWGG | 5 cut(s) | 347, 579, 645, 1143, 1775 |
| Bst1107I | GTATAC | 1 cut(s) | 2036 |
| Bst2UI | CCWGG | 5 cut(s) | 224, 308, 480, 868, 1950 |
| Bst4CI | ACNGT | 7 cut(s) | 516, 889, 910, 1962, 2181, 2276, 2423 |
| Bst6I | CTCTTC | 2 cut(s) | 1485, 2073 |
| BstAUI | TGTACA | 1 cut(s) | 1657 |
| BstC8I | GCNNGC | 2 cut(s) | 201, 555 |
| BstDSI | CCRYGG | 4 cut(s) | 347, 645, 1143, 2177 |
| BstF5I | GGATG | 5 cut(s) | 151, 643, 796, 844, 1671 |
| BstFNI | CGCG | 2 cut(s) | 1808, 1872 |
| BstHHI | GCGC | 2 cut(s) | 1281, 1671 |
| BstMAI | GTCTC | 3 cut(s) | 435, 1194, 2439 |
| BstMCI | CGRYCG | 1 cut(s) | 2278 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 100, 196, 536, 590 |
| BstNI | CCWGG | 5 cut(s) | 224, 308, 480, 868, 1950 |
| BstNSI | RCATGY | 3 cut(s) | 557, 974, 1313 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 517 |
| BstSCI | CCNGG | 8 cut(s) | 222, 240, 306, 478, 631, 632, 866, 1948 |
| BstSFI | CTRYAG | 1 cut(s) | 2466 |
| BstSLI | GKGCMC | 1 cut(s) | 135 |
| BstUI | CGCG | 2 cut(s) | 1808, 1872 |
| BstV1I | GCAGC | 5 cut(s) | 157, 517, 602, 1251, 1702 |
| BstX2I | RGATCY | 7 cut(s) | 612, 1039, 1255, 1453, 2150, 2173, 2249 |
| BstYI | RGATCY | 7 cut(s) | 612, 1039, 1255, 1453, 2150, 2173, 2249 |
| BstZ17I | GTATAC | 1 cut(s) | 2036 |
| Bsu15I | ATCGAT | 1 cut(s) | 1720 |
| BsuI | GTATCC | 1 cut(s) | 2294 |
| BsuRI | GGCC | 5 cut(s) | 103, 306, 412, 1387, 1994 |
| BsuTUI | ATCGAT | 1 cut(s) | 1720 |
| BtgI | CCRYGG | 4 cut(s) | 347, 645, 1143, 2177 |
| BtgZI | GCGATG | 1 cut(s) | 1661 |
| BtsCI | GGATG | 5 cut(s) | 151, 643, 796, 844, 1671 |
| BtsI | GCAGTG | 2 cut(s) | 783, 2422 |
| BtsIMutI | CAGTG | 7 cut(s) | 564, 783, 1188, 1221, 1845, 2002, 2422 |
| BtuMI | TCGCGA | 1 cut(s) | 1808 |
| Cac8I | GCNNGC | 2 cut(s) | 201, 555 |
| CaiI | CAGNNNCTG | 2 cut(s) | 170, 1259 |
| CciI | TCATGA | 1 cut(s) | 2431 |
| CfoI | GCGC | 2 cut(s) | 1281, 1671 |
| Cfr13I | GGNCC | 5 cut(s) | 101, 410, 519, 739, 2222 |
| Cfr9I | CCCGGG | 1 cut(s) | 632 |
| ClaI | ATCGAT | 1 cut(s) | 1720 |
| Csp6I | GTAC | 6 cut(s) | 628, 1214, 1658, 1977, 2424, 2471 |
| CviQI | GTAC | 6 cut(s) | 628, 1214, 1658, 1977, 2424, 2471 |
| DraI | TTTAAA | 1 cut(s) | 942 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 2377 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 2377 |
| EaeI | YGGCCR | 1 cut(s) | 304 |
| Eam1104I | CTCTTC | 2 cut(s) | 1485, 2073 |
| EarI | CTCTTC | 2 cut(s) | 1485, 2073 |
| EciI | GGCGGA | 1 cut(s) | 2186 |
| Eco130I | CCWWGG | 5 cut(s) | 347, 579, 645, 1143, 1775 |
| Eco31I | GGTCTC | 1 cut(s) | 1194 |
| Eco47I | GGWCC | 3 cut(s) | 519, 739, 2222 |
| Eco57I | CTGAAG | 2 cut(s) | 2142, 2175 |
| Eco88I | CYCGRG | 2 cut(s) | 632, 917 |
| EcoO109I | RGGNCCY | 2 cut(s) | 101, 739 |
| EcoRI | GAATTC | 1 cut(s) | 1077 |
| EcoRII | CCWGG | 5 cut(s) | 222, 306, 478, 866, 1948 |
| EcoT14I | CCWWGG | 5 cut(s) | 347, 579, 645, 1143, 1775 |
| ErhI | CCWWGG | 5 cut(s) | 347, 579, 645, 1143, 1775 |
| FaqI | GGGAC | 4 cut(s) | 455, 752, 1731, 1948 |
| FauNDI | CATATG | 4 cut(s) | 586, 1813, 2200, 2450 |
| FbaI | TGATCA | 1 cut(s) | 1150 |
| FblI | GTMKAC | 2 cut(s) | 1462, 2035 |
| Fnu4HI | GCNGC | 5 cut(s) | 171, 531, 591, 1265, 1691 |
| FokI | GGATG | 5 cut(s) | 158, 650, 783, 851, 1658 |
| Fsp4HI | GCNGC | 5 cut(s) | 171, 531, 591, 1265, 1691 |
| FspAI | RTGCGCAY | 1 cut(s) | 1280 |
| FspBI | CTAG | 8 cut(s) | 153, 191, 1082, 1631, 2004, 2051, 2115, 2313 |
| FspI | TGCGCA | 1 cut(s) | 1280 |
| GlaI | GCGC | 2 cut(s) | 1280, 1670 |
| GluI | GCNGC | 5 cut(s) | 171, 531, 591, 1265, 1691 |
| GsaI | CCCAGC | 1 cut(s) | 1969 |
| GsuI | CTGGAG | 1 cut(s) | 588 |
| HaeIII | GGCC | 5 cut(s) | 103, 306, 412, 1387, 1994 |
| HapII | CCGG | 2 cut(s) | 242, 633 |
| HhaI | GCGC | 2 cut(s) | 1281, 1671 |
| Hin6I | GCGC | 2 cut(s) | 1279, 1669 |
| HinP1I | GCGC | 2 cut(s) | 1279, 1669 |
| HincII | GTYRAC | 1 cut(s) | 1636 |
| HindII | GTYRAC | 1 cut(s) | 1636 |
| HindIII | AAGCTT | 1 cut(s) | 125 |
| HinfI | GANTC | 6 cut(s) | 218, 1103, 1160, 1333, 1435, 1744 |
| HpaII | CCGG | 2 cut(s) | 242, 633 |
| HphI | GGTGA | 5 cut(s) | 407, 442, 1531, 1675, 1898 |
| Hpy166II | GTNNAC | 8 cut(s) | 522, 1307, 1463, 1522, 1636, 1867, 1958, 2036 |
| Hpy188I | TCNGA | 4 cut(s) | 1132, 1171, 1561, 2155 |
| Hpy8I | GTNNAC | 8 cut(s) | 522, 1307, 1463, 1522, 1636, 1867, 1958, 2036 |
| HpyCH4III | ACNGT | 7 cut(s) | 516, 889, 910, 1962, 2181, 2276, 2423 |
| HpyCH4IV | ACGT | 1 cut(s) | 718 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 100, 196, 536, 590 |
| HpySE526I | ACGT | 1 cut(s) | 718 |
| HspAI | GCGC | 2 cut(s) | 1279, 1669 |
| KflI | GGGWCCC | 1 cut(s) | 739 |
| Ksp22I | TGATCA | 1 cut(s) | 1150 |
| LmnI | GCTCC | 5 cut(s) | 91, 1000, 1125, 1145, 1856 |
| Lsp1109I | GCAGC | 5 cut(s) | 157, 517, 602, 1251, 1702 |
| LweI | GCATC | 3 cut(s) | 157, 1610, 1680 |
| MaeI | CTAG | 8 cut(s) | 153, 191, 1082, 1631, 2004, 2051, 2115, 2313 |
| MaeII | ACGT | 1 cut(s) | 718 |
| MaeIII | GTNAC | 8 cut(s) | 370, 448, 617, 874, 1240, 1417, 2270, 2417 |
| MfeI | CAATTG | 1 cut(s) | 78 |
| MflI | RGATCY | 7 cut(s) | 612, 1039, 1255, 1453, 2150, 2173, 2249 |
| MhlI | GDGCHC | 1 cut(s) | 135 |
| MlsI | TGGCCA | 1 cut(s) | 306 |
| MluNI | TGGCCA | 1 cut(s) | 306 |
| MlyI | GAGTC | 4 cut(s) | 212, 1097, 1169, 1753 |
| MmeI | TCCRAC | 5 cut(s) | 415, 824, 1834, 2065, 2278 |
| Mox20I | TGGCCA | 1 cut(s) | 306 |
| MroXI | GAANNNNTTC | 2 cut(s) | 759, 1802 |
| MscI | TGGCCA | 1 cut(s) | 306 |
| MseI | TTAA | 6 cut(s) | 11, 71, 282, 941, 1365, 1593 |
| MslI | CAYNNNNRTG | 2 cut(s) | 986, 2436 |
| Msp20I | TGGCCA | 1 cut(s) | 306 |
| MspA1I | CMGCKG | 2 cut(s) | 455, 2396 |
| MspI | CCGG | 2 cut(s) | 242, 633 |
| MspR9I | CCNGG | 8 cut(s) | 224, 242, 308, 480, 633, 634, 868, 1950 |
| MunI | CAATTG | 1 cut(s) | 78 |
| Mva1269I | GAATGC | 4 cut(s) | 607, 810, 1802, 2330 |
| MvaI | CCWGG | 5 cut(s) | 224, 308, 480, 868, 1950 |
| MvnI | CGCG | 2 cut(s) | 1808, 1872 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 100, 196, 536, 590 |
| NciI | CCSGG | 3 cut(s) | 242, 633, 634 |
| NcoI | CCATGG | 3 cut(s) | 347, 645, 1143 |
| NdeI | CATATG | 4 cut(s) | 586, 1813, 2200, 2450 |
| NlaIV | GGNNCC | 6 cut(s) | 162, 411, 740, 741, 1858, 2175 |
| NmuCI | GTSAC | 3 cut(s) | 370, 448, 2417 |
| NruI | TCGCGA | 1 cut(s) | 1808 |
| NsbI | TGCGCA | 1 cut(s) | 1280 |
| NspI | RCATGY | 3 cut(s) | 557, 974, 1313 |
| PaeI | GCATGC | 1 cut(s) | 557 |
| PagI | TCATGA | 1 cut(s) | 2431 |
| PciI | ACATGT | 1 cut(s) | 970 |
| PctI | GAATGC | 4 cut(s) | 607, 810, 1802, 2330 |
| PdmI | GAANNNNTTC | 2 cut(s) | 759, 1802 |
| PfeI | GAWTC | 2 cut(s) | 1333, 1435 |
| PflFI | GACNNNGTC | 3 cut(s) | 446, 1106, 2336 |
| PkrI | GCNGC | 5 cut(s) | 172, 532, 592, 1266, 1692 |
| PleI | GAGTC | 4 cut(s) | 212, 1097, 1168, 1752 |
| PpsI | GAGTC | 4 cut(s) | 212, 1097, 1168, 1752 |
| PpuMI | RGGWCCY | 1 cut(s) | 739 |
| PscI | ACATGT | 1 cut(s) | 970 |
| PshAI | GACNNNNGTC | 1 cut(s) | 517 |
| PsiI | TTATAA | 1 cut(s) | 1469 |
| Psp5II | RGGWCCY | 1 cut(s) | 739 |
| Psp6I | CCWGG | 5 cut(s) | 222, 306, 478, 866, 1948 |
| PspFI | CCCAGC | 1 cut(s) | 1965 |
| PspGI | CCWGG | 5 cut(s) | 222, 306, 478, 866, 1948 |
| PspN4I | GGNNCC | 6 cut(s) | 162, 411, 740, 741, 1858, 2175 |
| PspPI | GGNCC | 5 cut(s) | 101, 410, 519, 739, 2222 |
| PspPPI | RGGWCCY | 1 cut(s) | 739 |
| PsrI | GAACNNNNNNTAC | 1 cut(s) | 2454 |
| PstNI | CAGNNNCTG | 2 cut(s) | 170, 1259 |
| PsuI | RGATCY | 7 cut(s) | 612, 1039, 1255, 1453, 2150, 2173, 2249 |
| PsyI | GACNNNGTC | 3 cut(s) | 446, 1106, 2336 |
| PvuII | CAGCTG | 1 cut(s) | 2396 |
| RruI | TCGCGA | 1 cut(s) | 1808 |
| RsaI | GTAC | 6 cut(s) | 629, 1215, 1659, 1978, 2425, 2472 |
| RsaNI | GTAC | 6 cut(s) | 628, 1214, 1658, 1977, 2424, 2471 |
| RseI | CAYNNNNRTG | 2 cut(s) | 986, 2436 |
| SaqAI | TTAA | 6 cut(s) | 11, 71, 282, 941, 1365, 1593 |
| SatI | GCNGC | 5 cut(s) | 171, 531, 591, 1265, 1691 |
| Sau96I | GGNCC | 5 cut(s) | 101, 410, 519, 739, 2222 |
| ScaI | AGTACT | 3 cut(s) | 1215, 1978, 2472 |
| SchI | GAGTC | 4 cut(s) | 212, 1097, 1169, 1753 |
| ScrFI | CCNGG | 8 cut(s) | 224, 242, 308, 480, 633, 634, 868, 1950 |
| SduI | GDGCHC | 1 cut(s) | 135 |
| SfaNI | GCATC | 3 cut(s) | 157, 1610, 1680 |
| SfcI | CTRYAG | 1 cut(s) | 2466 |
| SinI | GGWCC | 3 cut(s) | 519, 739, 2222 |
| SmaI | CCCGGG | 1 cut(s) | 634 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 986, 2436 |
| SmlI | CTYRAG | 4 cut(s) | 140, 332, 1209, 1610 |
| SmoI | CTYRAG | 4 cut(s) | 140, 332, 1209, 1610 |
| SphI | GCATGC | 1 cut(s) | 557 |
| SsiI | CCGC | 3 cut(s) | 453, 1870, 2171 |
| SspI | AATATT | 2 cut(s) | 9, 831 |
| SspMI | CTAG | 8 cut(s) | 153, 191, 1082, 1631, 2004, 2051, 2115, 2313 |
| StyD4I | CCNGG | 8 cut(s) | 222, 240, 306, 478, 631, 632, 866, 1948 |
| StyI | CCWWGG | 5 cut(s) | 347, 579, 645, 1143, 1775 |
| TaaI | ACNGT | 7 cut(s) | 516, 889, 910, 1962, 2181, 2276, 2423 |
| TaiI | ACGT | 1 cut(s) | 721 |
| TaqI | TCGA | 8 cut(s) | 511, 880, 1426, 1720, 2340, 2353, 2381, 2460 |
| TaqII | GACCGA | 1 cut(s) | 2210 |
| TatI | WGTACW | 4 cut(s) | 1213, 1657, 1976, 2470 |
| TfiI | GAWTC | 2 cut(s) | 1333, 1435 |
| Tru1I | TTAA | 6 cut(s) | 11, 71, 282, 941, 1365, 1593 |
| Tru9I | TTAA | 6 cut(s) | 11, 71, 282, 941, 1365, 1593 |
| TscAI | CASTG | 7 cut(s) | 571, 783, 1195, 1228, 1852, 2002, 2422 |
| TseFI | GTSAC | 3 cut(s) | 370, 448, 2417 |
| TseI | GCWGC | 5 cut(s) | 170, 530, 590, 1264, 1690 |
| Tsp45I | GTSAC | 3 cut(s) | 370, 448, 2417 |
| TspGWI | ACGGA | 1 cut(s) | 1049 |
| TspMI | CCCGGG | 1 cut(s) | 632 |
| TspRI | CASTG | 7 cut(s) | 571, 783, 1195, 1228, 1852, 2002, 2422 |
| Tth111I | GACNNNGTC | 3 cut(s) | 446, 1106, 2336 |
| VpaK11BI | GGWCC | 3 cut(s) | 519, 739, 2222 |
| XbaI | TCTAGA | 2 cut(s) | 1081, 2050 |
| XceI | RCATGY | 3 cut(s) | 557, 974, 1313 |
| XmaI | CCCGGG | 1 cut(s) | 632 |
| XmiI | GTMKAC | 2 cut(s) | 1462, 2035 |
| XmnI | GAANNNNTTC | 2 cut(s) | 759, 1802 |
| XspI | CTAG | 8 cut(s) | 153, 191, 1082, 1631, 2004, 2051, 2115, 2313 |
| ZrmI | AGTACT | 3 cut(s) | 1215, 1978, 2472 |