Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0002988

Physical Location & Seq

Genomic Coordinates Reverse (-)

4501870 .. 4507102

Gene Structure 5233 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1063300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2859 bp

ATGGCGGCATTACCATTCTCCGCGAAATCAACACCGTCAAAGTTCGTCTCAAGCTTCATACACTCCAACCGCATAAGAAGAAACTCGAGACCGTTTTTTATCACAACAACCACTTGTTCCCGCAGCCGAGCAAGGAGGCAGCTTCGTGTGAAGAACGTGGAGACTGATGAGAAAGATGTCGCCAGTCAAACCCAACAAGGCAGTTTGGCTACAATTCCTCCTGATTCTTCATTCATTGCATCGAATATCAAATACCATGCCGAATTCACACCCTCTTTCTCTATCGAATCTTTCGCGCTTCCCAAGGCATTCTACGGTACCGCTGAAAGTGTTCGTGACATGCTCATTGTTAATTGGAACGCTACATATGAGTACCATGACAGGCTGAATGTAAAGCAGGCATATTATCTCTCAATGGAGTTTCTACAGGGAAGAGCATTGCTGAATGCCATTGGTAACTTGGAGCTTTCGGGGGCTTATGCACATGCCTTGAAAACGCTAGGGCACAATCTAGAGGATGTAGCTAGGCAGGAACCGGATGCTGCGCTTGGAAATGGCGGGTTGGGGAGGCTTGCTTCCTGCTTTTTGGACTCCCTGGCCACTCAAAATTACCCGGCATGGGGATACGGACTCAGATACAAGTATGGCTTATTTAAACAGCACATTACAAAAGATGGGCAGGAGGAAGTTGCTGAAAATTGGCTGGAGATGGGAAATCCGTGGGAAATTCCAAGAAATGATGTGAGCTATCCTGTGAAATTCTACGGCGAAGTTGTTTCAGGGCTAGATGGAAAAAAAACATGGATTGGAGGCGAAAATGTCATGGCGGTTGCCTACGATGTCCCCATACCGGGATATAAAACTAAAACCACCATAAACCTTCGACTTTGGTCCACAAAAGTAGCACCGGAAGAGTTTGATTTACATGCTTTCAACACTGGAGATCATGCCAAGGCGTATGCAGCTTTAAAGAATGCCGAAAAGATCTGTTATATATTGTACCCTGGGGATGAATCCATGGAGGGAAAATCACTTCGGTTAAAGCAACAGTACACATTGTGTTCTGCTTCTCTCCAAGATATAGTTGCGCGGTTTGAGAGAAGATCGGGGGAGTCTGTGAAATGGGAGGAGTTTCCTGAGAAGGTTGCAGTGCAGATGAATGATACTCATCCAACTCTCTGCATCCCAGAGTTGATAAGGATATTGATGGACGTTAAGGGCTTGAGCTGGACGGAAGCCTGGGACATTACTCGAAGAACTGTTGCATACACTAATCATACAGTGTTACCTGAGGCATTGGAGAAATGGAGTTTGCAGCTCATACAGGAATTACTTCCTCGACACGTTGAGATCATAAAAACGATTGATGAGGAGCTAATTCATACCATTATTGCTGAGTACGGCACAGAAGATCTTAACTTGTTGGAACAAAAACTGAGAGAAATGAGAATTCTTGATAATATCGATTTGCCTGATTCAGTCATTGGAATTCTCTGTAAATCAGAAGAAAGTTCTGCAGTTGATCACGTTGAGGAAGTTGATTTTTCTGATGAAGAAGCTAAAACCACTGATGAAGTAGGTCAATCTAAAGGACTAGACACTAATACAAAAAATGAGGTTACATTTGAACCGGATCCTAAACTTCCAAAGACGGTGAGAATGGCTAATCTATGCATTGCTGGTGGACATGCAGTAAACGGCGTTGCTGAGATTCACAGCGAAATAGTGAAGAACGAAGTATTTAACGATTTCTATAAGTTGTGGCCTGAGAAATTTCAAAACAAGACAAATGGGGTGACACCGAGAAGATGGATTCGTTTCTGCAATCCAGGTCTGAGTAAAATTATAACCAAATGGATTGGAACAGACGATTGGGTGAGAAATACTAACATGTTGGTCACCCTTCGGAAGTTTGCTGATAATGAAGATCTCCAATCCGAATGGAGGGAAGCGAAAAGGAGAAACAAGATTAAGGTTGCATCCTTCCTCAAGGAAAAAACTGGTTTTCTAGTCAACCCTGACGCAATGTTTGATGTGCAAGTGAAGCGCATTCATGAATACAAACGGCAGCTATTGAACATCCTGGGTATCATTTATCGCTACAAGAAAATGAAGGAAATGAGTCCTGAGGAAAGAAAAGCAAAGTTTGTTCCAAGGGTGTGTATATTTGGAGGAAAGGCATTCGCAACCTATATCCAAGCGAAAAGGATTGTGAAATTTATCACAGATGTTGGAGCCACTGTGAATAACGATCGAGACATAGGTGACCTTTTGAAGGTTGTGTTTGTTCCTGACTACAATGTCAGTGTTGCTGAAGTGCTTATTCCCGGTAGTGAGCTCTCCCAGCATATCAGCACTGCTGGAATGGAGGCGAGTGGAACTAGCAACATGAAATTTGCAATGAATGGTTGCGTACAAATTGGAACCCTAGATGGGGCAAATGTCGAAATAAGAGAAGAGGTTGGAGAGGACAATTTTTTCCTCTTTGGTGCACATGCTCATGAAATTGCCGGGCTAAGGAAAGAAAGATCTGAAGGAAAGTTCGTTCCGGACCCGCGGTTCGAAGAAGTGAAGGCATATGTGAGAACCGGCGTGTTTGGTCCTTACAACTATGATGAACTCATGGGATCATTGGAAGGAAATGAAGGTTATGGTCGTGCTGATTACTTTCTCGTTGGCAAGGACTTCCCTAGTTACCTTGAGTGCCAAGACAAGGTTGATGAAGCATATCGAGACCAAAAGAGATGGACGAGGAAGTCGATTTTGAACACAGCTGGTTCGTACAAGTTCAGCAGTGACCGTACAATTCATGAATACGCGAGGGACATATGGAAGATTGAACCTGTTGTTTTACAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009501 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 ko02026 ko04217 ko04910 ko04922 ko04931 map00500 map01100 map01110 map02026 map04217 map04910 map04922 map04931

Pfam Domains

Protein Families

Phosphorylase

Protein Analysis

953

Amino Acids

108.17

Weight (kDa)

6.08

Isoelectric Point (pI)

41.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Phosphorylase	PF00343	164 - 486	2.2e-128	Carbohydrate phosphorylase
Phosphorylase	PF00343	548 - 946	9.6e-165	Carbohydrate phosphorylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002988)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G29320
fragaria_vesca	FvH4_4g30370 FvH4_6g47260 FvH4_6g47260 FvH4_6g47260 FvH4_6g47260
malus_domestica	MD00G1156200.v1.1 MD09G1062600.v1.1 MD16G1063300.v1.1
prunus_persica	Prupe.1G283400_v2.0.a1 Prupe.1G283400_v2.0.a1 Prupe.3G257600_v2.0.a1
pyrus_communis	pycom111g05290
rosa_chinensis	RchiOBHm_Chr2g0166331 RchiOBHm_Chr4g0438671
rosa_laevigata	RLG00000006342 RLG00000021610
rosa_multiflora	Rmu_sc0000738.1_g000042 Rmu_sc0002505.1_g000002 Rmu_sc0007974.1_g000007
rosa_roxburghii	Rroxscaffold_2G00084990 Rroxscaffold_5G00379690 Rroxscaffold_7G00200130
rosa_rugosa	Rorug02G0522200 Rorug04G0312500 Rorug04G0312600 Rorug04G0312600
rosa_samantha	Rh2AG588200 Rh2BG600200 Rh2CG571100 Rh2DG610400 Rh4AG363000 Rh4BG374300 Rh4CG388400 Rh4DG368900
rosa_wichuraiana	Rw0G007140 Rw0G016690 Rw2G049020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1847
AasI	GACNNNNNNGTC	2 cut(s)	2300, 2755
Acc65I	GGTACC	1 cut(s)	317
AccB1I	GGYRCC	1 cut(s)	317
AccII	CGCG	5 cut(s)	23, 296, 1090, 2557, 2819
AccIII	TCCGGA	1 cut(s)	2548
AclWI	GGATC	3 cut(s)	1628, 1641, 2635
AcoI	YGGCCR	1 cut(s)	597
AcsI	RAATTY	8 cut(s)	263, 726, 758, 1449, 1488, 1772, 2216, 2393
AcuI	CTGAAG	2 cut(s)	2334, 2553
AdeI	CACNNNGTG	1 cut(s)	1059
AfaI	GTAC	8 cut(s)	319, 374, 1001, 1052, 1400, 2415, 2783, 2803
AflIII	ACRYGT	2 cut(s)	1342, 1890
AgsI	TTSAA	8 cut(s)	493, 934, 1628, 1778, 2077, 2275, 2767, 2840
AjnI	CCWGG	5 cut(s)	594, 1003, 1238, 1828, 2082
AjuI	GAANNNNNNNTTGG	2 cut(s)	1855, 1887
AloI	GAACNNNNNNTCC	4 cut(s)	2529, 2543, 2561, 2575
Alw21I	GWGCWC	2 cut(s)	2340, 2494
Alw26I	GTCTC	5 cut(s)	52, 82, 155, 2250, 2727
Alw44I	GTGCAC	1 cut(s)	2490
AlwI	GGATC	3 cut(s)	1628, 1641, 2635
Ama87I	CYCGRG	1 cut(s)	85
Aor13HI	TCCGGA	1 cut(s)	2548
AoxI	GGCC	2 cut(s)	597, 1763
ApaLI	GTGCAC	1 cut(s)	2490
ApeKI	GCWGC	6 cut(s)	123, 139, 542, 962, 1315, 2068
ApoI	RAATTY	8 cut(s)	263, 726, 758, 1449, 1488, 1772, 2216, 2393
ArsI	GACNNNNNNTTYG	4 cut(s)	2012, 2044, 2543, 2575
Asp700I	GAANNNNTTC	2 cut(s)	330, 1332
Asp718I	GGTACC	1 cut(s)	317
AspLEI	GCGC	4 cut(s)	298, 547, 1090, 2049
AspS9I	GGNCC	3 cut(s)	891, 2551, 2600
AsuC2I	CCSGG	4 cut(s)	614, 852, 2328, 2512
AsuHPI	GGTGA	5 cut(s)	1666, 1807, 1888, 1891, 2276
AsuII	TTCGAA	1 cut(s)	2562
AvaI	CYCGRG	1 cut(s)	85
AvaII	GGWCC	3 cut(s)	891, 2551, 2600
AxyI	CCTNAGG	2 cut(s)	1290, 2127
BaeGI	GKGCMC	2 cut(s)	507, 2494
BalI	TGGCCA	1 cut(s)	599
BamHI	GGATCC	1 cut(s)	1633
BanI	GGYRCC	1 cut(s)	317
BanII	GRGCYC	1 cut(s)	2340
Bbv12I	GWGCWC	2 cut(s)	2340, 2494
BbvI	GCAGC	6 cut(s)	135, 151, 529, 974, 1327, 2080
BccI	CCATC	7 cut(s)	668, 703, 782, 1201, 1803, 2426, 2739
BceAI	ACGGC	4 cut(s)	781, 1417, 1714, 2081
BciT130I	CCWGG	5 cut(s)	596, 1005, 1240, 1830, 2084
BciVI	GTATCC	1 cut(s)	617
BclI	TGATCA	1 cut(s)	1522
BcnI	CCSGG	4 cut(s)	614, 852, 2328, 2512
BcoDI	GTCTC	5 cut(s)	52, 82, 155, 2250, 2727
BfaI	CTAG	9 cut(s)	500, 512, 525, 785, 1595, 2009, 2382, 2429, 2691
BfmI	CTRYAG	2 cut(s)	425, 1515
BfuI	GTATCC	1 cut(s)	617
BglII	AGATCT	4 cut(s)	984, 1411, 1927, 2528
BisI	GCNGC	7 cut(s)	6, 124, 140, 543, 963, 1316, 2069
BlsI	GCNGC	7 cut(s)	7, 125, 141, 544, 964, 1317, 2070
Bme1390I	CCNGG	9 cut(s)	596, 614, 852, 1005, 1240, 1830, 2084, 2328, 2512
Bme18I	GGWCC	3 cut(s)	891, 2551, 2600
BmeT110I	CYCGRG	1 cut(s)	85
BmgT120I	GGNCC	3 cut(s)	891, 2551, 2600
BmiI	GGNNCC	6 cut(s)	319, 534, 1635, 2236, 2425, 2553
BmrFI	CCNGG	9 cut(s)	596, 614, 852, 1005, 1240, 1830, 2084, 2328, 2512
BmsI	GCATC	4 cut(s)	248, 529, 1191, 1988
BoxI	GACNNNNGTC	1 cut(s)	889
BpmI	CTGGAG	2 cut(s)	725, 960
Bpu10I	CCTNAGC	1 cut(s)	2516
Bpu14I	TTCGAA	1 cut(s)	2562
BpuEI	CTTGAG	4 cut(s)	34, 1243, 1973, 2720
BpuMI	CCSGG	4 cut(s)	614, 852, 2328, 2512
Bsa29I	ATCGAT	1 cut(s)	1464
BsaBI	GATNNNNATC	1 cut(s)	1167
BsaI	GGTCTC	2 cut(s)	82, 2727
BsaWI	WCCGGW	4 cut(s)	535, 907, 1630, 2548
BsaXI	ACNNNNNCTCC	4 cut(s)	202, 232, 2226, 2256
Bse118I	RCCGGY	1 cut(s)	2588
Bse1I	ACTGG	3 cut(s)	183, 943, 2005
Bse21I	CCTNAGG	2 cut(s)	1290, 2127
Bse3DI	GCAATG	5 cut(s)	234, 437, 1674, 2031, 2406
Bse8I	GATNNNNATC	1 cut(s)	1167
BseAI	TCCGGA	1 cut(s)	2548
BseBI	CCWGG	5 cut(s)	596, 1005, 1240, 1830, 2084
BseCI	ATCGAT	1 cut(s)	1464
BseGI	GGATG	7 cut(s)	523, 544, 1015, 1168, 1182, 1979, 2079
BseJI	GATNNNNATC	1 cut(s)	1167
BseMI	GCAATG	5 cut(s)	234, 437, 1674, 2031, 2406
BseMII	CTCAG	9 cut(s)	646, 1128, 1281, 1386, 1427, 1698, 1758, 1826, 2118
BseNI	ACTGG	3 cut(s)	183, 943, 2005
BseRI	GAGGAG	2 cut(s)	1142, 1385
BseSI	GKGCMC	2 cut(s)	507, 2494
BseXI	GCAGC	6 cut(s)	135, 151, 529, 974, 1327, 2080
BseYI	CCCAGC	1 cut(s)	2343
BsgI	GTGCAG	1 cut(s)	1172
Bsh1236I	CGCG	5 cut(s)	23, 296, 1090, 2557, 2819
Bsh1285I	CGRYCG	1 cut(s)	2254
BshFI	GGCC	2 cut(s)	599, 1765
BshNI	GGYRCC	1 cut(s)	317
BshVI	ATCGAT	1 cut(s)	1464
BsiEI	CGRYCG	1 cut(s)	2254
BsiHKAI	GWGCWC	2 cut(s)	2340, 2494
BsiHKCI	CYCGRG	1 cut(s)	85
BsiSI	CCGG	9 cut(s)	536, 614, 851, 908, 1631, 2328, 2511, 2549, 2589
BslFI	GGGAC	3 cut(s)	827, 1256, 2837
BsmAI	GTCTC	5 cut(s)	52, 82, 155, 2250, 2727
BsmBI	CGTCTC	1 cut(s)	52
BsmFI	GGGAC	3 cut(s)	827, 1256, 2837
BsmI	GAATGC	5 cut(s)	308, 451, 979, 2049, 2180
BsnI	GGCC	2 cut(s)	599, 1765
Bso31I	GGTCTC	2 cut(s)	82, 2727
BsoBI	CYCGRG	1 cut(s)	85
Bsp119I	TTCGAA	1 cut(s)	2562
Bsp1286I	GDGCHC	3 cut(s)	507, 2340, 2494
Bsp13I	TCCGGA	1 cut(s)	2548
Bsp19I	CCATGG	1 cut(s)	1017
BspANI	GGCC	2 cut(s)	599, 1765
BspCNI	CTCAG	9 cut(s)	645, 1129, 1282, 1387, 1428, 1699, 1759, 1827, 2119
BspDI	ATCGAT	1 cut(s)	1464
BspEI	TCCGGA	1 cut(s)	2548
BspFNI	CGCG	5 cut(s)	23, 296, 1090, 2557, 2819
BspHI	TCATGA	3 cut(s)	2053, 2500, 2809
BspLI	GGNNCC	6 cut(s)	319, 534, 1635, 2236, 2425, 2553
BspMAI	CTGCAG	1 cut(s)	1519
BspPI	GGATC	3 cut(s)	1628, 1641, 2635
BspQI	GCTCTTC	1 cut(s)	427
BspT104I	TTCGAA	1 cut(s)	2562
BspT107I	GGYRCC	1 cut(s)	317
BspTNI	GGTCTC	2 cut(s)	82, 2727
BsrDI	GCAATG	5 cut(s)	234, 437, 1674, 2031, 2406
BsrFI	RCCGGY	1 cut(s)	2588
BsrI	ACTGG	3 cut(s)	183, 943, 2005
BssAI	RCCGGY	1 cut(s)	2588
BssT1I	CCWWGG	4 cut(s)	303, 951, 1017, 2153
Bst2UI	CCWGG	5 cut(s)	596, 1005, 1240, 1830, 2084
Bst6I	CTCTTC	3 cut(s)	427, 906, 2451
BstBI	TTCGAA	1 cut(s)	2562
BstC8I	GCNNGC	2 cut(s)	399, 573
BstDSI	CCRYGG	3 cut(s)	719, 1017, 2555
BstEII	GGTNACC	2 cut(s)	1897, 2264
BstENI	CCTNNNNNAGG	1 cut(s)	2273
BstF5I	GGATG	7 cut(s)	523, 544, 1015, 1168, 1182, 1979, 2079
BstFNI	CGCG	5 cut(s)	23, 296, 1090, 2557, 2819
BstHHI	GCGC	4 cut(s)	298, 547, 1090, 2049
BstMAI	GTCTC	5 cut(s)	52, 82, 155, 2250, 2727
BstMCI	CGRYCG	1 cut(s)	2254
BstMWI	GCNNNNNNNGC	3 cut(s)	962, 2044, 2344
BstNI	CCWGG	5 cut(s)	596, 1005, 1240, 1830, 2084
BstNSI	RCATGY	6 cut(s)	343, 488, 929, 1691, 1894, 2498
BstPAI	GACNNNNGTC	1 cut(s)	889
BstPI	GGTNACC	2 cut(s)	1897, 2264
BstSCI	CCNGG	9 cut(s)	594, 612, 850, 1003, 1238, 1828, 2082, 2326, 2510
BstSFI	CTRYAG	2 cut(s)	425, 1515
BstSLI	GKGCMC	2 cut(s)	507, 2494
BstUI	CGCG	5 cut(s)	23, 296, 1090, 2557, 2819
BstV1I	GCAGC	6 cut(s)	135, 151, 529, 974, 1327, 2080
BstX2I	RGATCY	5 cut(s)	984, 1411, 1633, 1927, 2528
BstYI	RGATCY	5 cut(s)	984, 1411, 1633, 1927, 2528
Bsu15I	ATCGAT	1 cut(s)	1464
Bsu36I	CCTNAGG	2 cut(s)	1290, 2127
BsuI	GTATCC	1 cut(s)	617
BsuRI	GGCC	2 cut(s)	599, 1765
BsuTUI	ATCGAT	1 cut(s)	1464
BtgI	CCRYGG	3 cut(s)	719, 1017, 2555
BtsCI	GGATG	7 cut(s)	523, 544, 1015, 1168, 1182, 1979, 2079
BtsI	GCAGTG	3 cut(s)	1155, 2355, 2800
BtsIMutI	CAGTG	8 cut(s)	936, 1155, 1287, 1566, 2238, 2311, 2355, 2800
Cac8I	GCNNGC	2 cut(s)	399, 573
CciI	TCATGA	3 cut(s)	2053, 2500, 2809
CfoI	GCGC	4 cut(s)	298, 547, 1090, 2049
Cfr10I	RCCGGY	1 cut(s)	2588
Cfr13I	GGNCC	3 cut(s)	891, 2551, 2600
Cfr42I	CCGCGG	1 cut(s)	2558
ClaI	ATCGAT	1 cut(s)	1464
CseI	GACGC	1 cut(s)	2030
Csp6I	GTAC	8 cut(s)	318, 373, 1000, 1051, 1399, 2414, 2782, 2802
CviQI	GTAC	8 cut(s)	318, 373, 1000, 1051, 1399, 2414, 2782, 2802
DraI	TTTAAA	2 cut(s)	655, 969
DraIII	CACNNNGTG	1 cut(s)	1059
DrdI	GACNNNNNNGTC	2 cut(s)	2300, 2755
DseDI	GACNNNNNNGTC	2 cut(s)	2300, 2755
EaeI	YGGCCR	1 cut(s)	597
Eam1104I	CTCTTC	3 cut(s)	427, 906, 2451
EarI	CTCTTC	3 cut(s)	427, 906, 2451
Ecl136II	GAGCTC	1 cut(s)	2338
Eco130I	CCWWGG	4 cut(s)	303, 951, 1017, 2153
Eco24I	GRGCYC	1 cut(s)	2340
Eco31I	GGTCTC	2 cut(s)	82, 2727
Eco47I	GGWCC	3 cut(s)	891, 2551, 2600
Eco53kI	GAGCTC	1 cut(s)	2338
Eco57I	CTGAAG	2 cut(s)	2334, 2553
Eco81I	CCTNAGG	2 cut(s)	1290, 2127
Eco88I	CYCGRG	1 cut(s)	85
Eco91I	GGTNACC	2 cut(s)	1897, 2264
EcoICRI	GAGCTC	1 cut(s)	2338
EcoNI	CCTNNNNNAGG	1 cut(s)	2273
EcoO65I	GGTNACC	2 cut(s)	1897, 2264
EcoRI	GAATTC	3 cut(s)	263, 1449, 1488
EcoRII	CCWGG	5 cut(s)	594, 1003, 1238, 1828, 2082
EcoT14I	CCWWGG	4 cut(s)	303, 951, 1017, 2153
EcoT22I	ATGCAT	1 cut(s)	1676
EcoT38I	GRGCYC	1 cut(s)	2340
ErhI	CCWWGG	4 cut(s)	303, 951, 1017, 2153
Esp3I	CGTCTC	1 cut(s)	52
FalI	AAGNNNNNCTT	2 cut(s)	632, 664
FaqI	GGGAC	3 cut(s)	827, 1256, 2837
FauI	CCCGC	3 cut(s)	128, 551, 2562
FauNDI	CATATG	3 cut(s)	367, 2578, 2828
FbaI	TGATCA	1 cut(s)	1522
Fnu4HI	GCNGC	7 cut(s)	6, 124, 140, 543, 963, 1316, 2069
FokI	GGATG	7 cut(s)	530, 551, 1022, 1155, 1169, 1966, 2066
FriOI	GRGCYC	1 cut(s)	2340
Fsp4HI	GCNGC	7 cut(s)	6, 124, 140, 543, 963, 1316, 2069
FspBI	CTAG	9 cut(s)	500, 512, 525, 785, 1595, 2009, 2382, 2429, 2691
GlaI	GCGC	4 cut(s)	297, 546, 1089, 2048
GluI	GCNGC	7 cut(s)	6, 124, 140, 543, 963, 1316, 2069
GsaI	CCCAGC	1 cut(s)	2347
GsuI	CTGGAG	2 cut(s)	725, 960
HaeIII	GGCC	2 cut(s)	599, 1765
HapII	CCGG	9 cut(s)	536, 614, 851, 908, 1631, 2328, 2511, 2549, 2589
HgaI	GACGC	1 cut(s)	2030
HhaI	GCGC	4 cut(s)	298, 547, 1090, 2049
Hin6I	GCGC	4 cut(s)	296, 545, 1088, 2047
HinP1I	GCGC	4 cut(s)	296, 545, 1088, 2047
HincII	GTYRAC	1 cut(s)	2014
HindII	GTYRAC	1 cut(s)	2014
HindIII	AAGCTT	1 cut(s)	52
HpaII	CCGG	9 cut(s)	536, 614, 851, 908, 1631, 2328, 2511, 2549, 2589
HphI	GGTGA	5 cut(s)	1666, 1807, 1888, 1891, 2276
Hpy166II	GTNNAC	6 cut(s)	894, 1053, 1685, 1696, 2014, 2492
Hpy188I	TCNGA	7 cut(s)	635, 1504, 1549, 1836, 1908, 1939, 2533
Hpy8I	GTNNAC	6 cut(s)	894, 1053, 1685, 1696, 2014, 2492
HpyCH4IV	ACGT	4 cut(s)	156, 1212, 1344, 1527
HpyF10VI	GCNNNNNNNGC	3 cut(s)	962, 2044, 2344
HpySE526I	ACGT	4 cut(s)	156, 1212, 1344, 1527
HspAI	GCGC	4 cut(s)	296, 545, 1088, 2047
Kpn2I	TCCGGA	1 cut(s)	2548
KpnI	GGTACC	1 cut(s)	321
Ksp22I	TGATCA	1 cut(s)	1522
KspI	CCGCGG	1 cut(s)	2558
LguI	GCTCTTC	1 cut(s)	427
LmnI	GCTCC	3 cut(s)	463, 1372, 2234
Lsp1109I	GCAGC	6 cut(s)	135, 151, 529, 974, 1327, 2080
LweI	GCATC	4 cut(s)	248, 529, 1191, 1988
MaeI	CTAG	9 cut(s)	500, 512, 525, 785, 1595, 2009, 2382, 2429, 2691
MaeII	ACGT	4 cut(s)	156, 1212, 1344, 1527
MaeIII	GTNAC	9 cut(s)	335, 455, 1284, 1618, 1795, 1897, 2264, 2693, 2795
MflI	RGATCY	5 cut(s)	984, 1411, 1633, 1927, 2528
MhlI	GDGCHC	3 cut(s)	507, 2340, 2494
MlsI	TGGCCA	1 cut(s)	599
MluNI	TGGCCA	1 cut(s)	599
MlyI	GAGTC	4 cut(s)	584, 624, 1121, 2131
MmeI	TCCRAC	5 cut(s)	90, 1196, 1404, 2212, 2443
Mox20I	TGGCCA	1 cut(s)	599
Mph1103I	ATGCAT	1 cut(s)	1676
MroI	TCCGGA	1 cut(s)	2548
MroXI	GAANNNNTTC	2 cut(s)	330, 1332
MscI	TGGCCA	1 cut(s)	599
MseI	TTAA	8 cut(s)	351, 654, 968, 1040, 1215, 1416, 1743, 1971
MslI	CAYNNNNRTG	1 cut(s)	2499
Msp20I	TGGCCA	1 cut(s)	599
MspA1I	CMGCKG	3 cut(s)	323, 2557, 2774
MspI	CCGG	9 cut(s)	536, 614, 851, 908, 1631, 2328, 2511, 2549, 2589
MspR9I	CCNGG	9 cut(s)	596, 614, 852, 1005, 1240, 1830, 2084, 2328, 2512
Mva1269I	GAATGC	5 cut(s)	308, 451, 979, 2049, 2180
MvaI	CCWGG	5 cut(s)	596, 1005, 1240, 1830, 2084
MvnI	CGCG	5 cut(s)	23, 296, 1090, 2557, 2819
MwoI	GCNNNNNNNGC	3 cut(s)	962, 2044, 2344
NciI	CCSGG	4 cut(s)	614, 852, 2328, 2512
NcoI	CCATGG	1 cut(s)	1017
NdeI	CATATG	3 cut(s)	367, 2578, 2828
NlaIV	GGNNCC	6 cut(s)	319, 534, 1635, 2236, 2425, 2553
NmeAIII	GCCGAG	1 cut(s)	152
NmuCI	GTSAC	5 cut(s)	335, 1795, 1897, 2264, 2795
NsiI	ATGCAT	1 cut(s)	1676
NspI	RCATGY	6 cut(s)	343, 488, 929, 1691, 1894, 2498
NspV	TTCGAA	1 cut(s)	2562
PaeR7I	CTCGAG	1 cut(s)	85
PagI	TCATGA	3 cut(s)	2053, 2500, 2809
PasI	CCCWGGG	1 cut(s)	1004
PciI	ACATGT	1 cut(s)	1890
PciSI	GCTCTTC	1 cut(s)	427
PcsI	WCGNNNNNNNCGW	1 cut(s)	2756
PctI	GAATGC	5 cut(s)	308, 451, 979, 2049, 2180
PdmI	GAANNNNTTC	2 cut(s)	330, 1332
PfeI	GAWTC	6 cut(s)	224, 287, 1013, 1475, 1711, 1813
PkrI	GCNGC	7 cut(s)	7, 125, 141, 544, 964, 1317, 2070
Ple19I	CGATCG	1 cut(s)	2254
PleI	GAGTC	4 cut(s)	584, 624, 1120, 2130
PpsI	GAGTC	4 cut(s)	584, 624, 1120, 2130
PscI	ACATGT	1 cut(s)	1890
PshAI	GACNNNNGTC	1 cut(s)	889
PsiI	TTATAA	1 cut(s)	1847
Psp124BI	GAGCTC	1 cut(s)	2340
Psp6I	CCWGG	5 cut(s)	594, 1003, 1238, 1828, 2082
PspEI	GGTNACC	2 cut(s)	1897, 2264
PspFI	CCCAGC	1 cut(s)	2343
PspGI	CCWGG	5 cut(s)	594, 1003, 1238, 1828, 2082
PspN4I	GGNNCC	6 cut(s)	319, 534, 1635, 2236, 2425, 2553
PspPI	GGNCC	3 cut(s)	891, 2551, 2600
PstI	CTGCAG	1 cut(s)	1519
PsuI	RGATCY	5 cut(s)	984, 1411, 1633, 1927, 2528
PvuI	CGATCG	1 cut(s)	2254
PvuII	CAGCTG	1 cut(s)	2774
RsaI	GTAC	8 cut(s)	319, 374, 1001, 1052, 1400, 2415, 2783, 2803
RsaNI	GTAC	8 cut(s)	318, 373, 1000, 1051, 1399, 2414, 2782, 2802
RseI	CAYNNNNRTG	1 cut(s)	2499
SacI	GAGCTC	1 cut(s)	2340
SacII	CCGCGG	1 cut(s)	2558
SapI	GCTCTTC	1 cut(s)	427
SaqAI	TTAA	8 cut(s)	351, 654, 968, 1040, 1215, 1416, 1743, 1971
SatI	GCNGC	7 cut(s)	6, 124, 140, 543, 963, 1316, 2069
Sau96I	GGNCC	3 cut(s)	891, 2551, 2600
SchI	GAGTC	4 cut(s)	584, 624, 1121, 2131
ScrFI	CCNGG	9 cut(s)	596, 614, 852, 1005, 1240, 1830, 2084, 2328, 2512
SduI	GDGCHC	3 cut(s)	507, 2340, 2494
SfaNI	GCATC	4 cut(s)	248, 529, 1191, 1988
SfcI	CTRYAG	2 cut(s)	425, 1515
Sfr274I	CTCGAG	1 cut(s)	85
Sfr303I	CCGCGG	1 cut(s)	2558
SfuI	TTCGAA	1 cut(s)	2562
SgrBI	CCGCGG	1 cut(s)	2558
SinI	GGWCC	3 cut(s)	891, 2551, 2600
SlaI	CTCGAG	1 cut(s)	85
SmiMI	CAYNNNNRTG	1 cut(s)	2499
SmlI	CTYRAG	5 cut(s)	49, 85, 1222, 1988, 2699
SmoI	CTYRAG	5 cut(s)	49, 85, 1222, 1988, 2699
SspMI	CTAG	9 cut(s)	500, 512, 525, 785, 1595, 2009, 2382, 2429, 2691
SstI	GAGCTC	1 cut(s)	2340
StyD4I	CCNGG	9 cut(s)	594, 612, 850, 1003, 1238, 1828, 2082, 2326, 2510
StyI	CCWWGG	4 cut(s)	303, 951, 1017, 2153
TaiI	ACGT	4 cut(s)	159, 1215, 1347, 1530
TatI	WGTACW	1 cut(s)	1050
TauI	GCSGC	1 cut(s)	8
TfiI	GAWTC	6 cut(s)	224, 287, 1013, 1475, 1711, 1813
Tru1I	TTAA	8 cut(s)	351, 654, 968, 1040, 1215, 1416, 1743, 1971
Tru9I	TTAA	8 cut(s)	351, 654, 968, 1040, 1215, 1416, 1743, 1971
TscAI	CASTG	8 cut(s)	943, 1155, 1287, 1573, 2245, 2311, 2362, 2800
TseFI	GTSAC	5 cut(s)	335, 1795, 1897, 2264, 2795
TseI	GCWGC	6 cut(s)	123, 139, 542, 962, 1315, 2068
Tsp45I	GTSAC	5 cut(s)	335, 1795, 1897, 2264, 2795
TspGWI	ACGGA	3 cut(s)	642, 708, 1247
TspRI	CASTG	8 cut(s)	943, 1155, 1287, 1573, 2245, 2311, 2362, 2800
VneI	GTGCAC	1 cut(s)	2490
VpaK11BI	GGWCC	3 cut(s)	891, 2551, 2600
XagI	CCTNNNNNAGG	1 cut(s)	2273
XapI	RAATTY	8 cut(s)	263, 726, 758, 1449, 1488, 1772, 2216, 2393
XbaI	TCTAGA	1 cut(s)	511
XceI	RCATGY	6 cut(s)	343, 488, 929, 1691, 1894, 2498
XcmI	CCANNNNNNNNNTGG	1 cut(s)	457
XhoI	CTCGAG	1 cut(s)	85
XmnI	GAANNNNTTC	2 cut(s)	330, 1332
XspI	CTAG	9 cut(s)	500, 512, 525, 785, 1595, 2009, 2382, 2429, 2691
Zsp2I	ATGCAT	1 cut(s)	1676

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.