Rorug04G0312600

Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002988
Physical Location & Seq
Forward (+)
48799868 .. 48802626
2759 bp
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UTR
Exon/CDS
Intron
Rorug04G0312600.1

Sequence Viewer

Length: 693 bp
ATGGGTTCTCTTTGCTGCTGTCCATGTGGTGATGAATTTGAAGAATATGCTCTTCCAAGCAATCCAGTGTACAGGCATTGCGCGTGCCTCAGATACTTCTTCCACCAGCTATTCAGCGGGTATAGTGCACCGTTTCAAAGGCTTGATGGGAGATCAGTCCCGTCACCTAACCAAGGGGCTACTTCTTCTGTCCCATCTGGAACTGGCACAACAGTAGCTAATAACTCCTCAAATGAGACTCAATTCTCAGTGGCCAGGCCATCACCCTTTGATGCCGATCAGAGGTACTCACGTTTGCAGCGCGACGGCTTGATCTCTAGGCGTGAGAAGTCAATGACACATTTGCAGGAAGATGCACAACAAGTGAGAAGAAGCAGTTCTGGGACAGAGTCCCTGGGTTCGGGGAAGAAACGGAATGGAGTTGATACTGAAGAAGATTGCAAGTTAGGTCAATCAGAGACCTCAGAAAAGGATCTAGCATCAAAAGTCGCATATGGACTAACTTATGTGCAGTCATCTTCTGAAGATGATGATGTCTGCCCTACATGTCTTGATGATTATACCCCTGAAAATCCTAAAATCACAACAAGATGTTCTCACCATTTCCATCTTGGCTGTATTTATGAGTGGTTGGAAAGAAGTGAAAGTTGTCCAATGTGTGGAAAGGAGATGGAGTTCTGTGAAAGCCCCTAA

Protein Analysis

230

Amino Acids

25.56

Weight (kDa)

5.09

Isoelectric Point (pI)

65.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 177 - 220 4.6e-11 RING-H2 zinc finger domain
zf-ANAPC11 PF12861 177 - 226 2.3e-07 Anaphase-promoting complex subunit 11 RING-H2 finger
zf-RING_2 PF13639 179 - 220 1.6e-11 Ring finger domain
zf-C3HC4_2 PF13923 180 - 220 9e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 180 - 220 1.1e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 180 - 207 8.1e-06 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 659
AccII CGCG 2 cut(s) 83, 303
AciI CCGC 1 cut(s) 117
AclWI GGATC 1 cut(s) 480
AcoI YGGCCR 1 cut(s) 252
AcsI RAATTY 1 cut(s) 35
AcuI CTGAAG 2 cut(s) 450, 543
AfaI GTAC 2 cut(s) 71, 287
AfiI CCNNNNNNNGG 4 cut(s) 173, 282, 400, 659
AflIII ACRYGT 1 cut(s) 545
AgsI TTSAA 2 cut(s) 41, 137
AjnI CCWGG 2 cut(s) 254, 393
AloI GAACNNNNNNTCC 2 cut(s) 659, 691
AluBI AGCT 2 cut(s) 109, 218
AluI AGCT 2 cut(s) 109, 218
Alw21I GWGCWC 1 cut(s) 130
Alw26I GTCTC 2 cut(s) 230, 452
Alw44I GTGCAC 1 cut(s) 126
AlwI GGATC 1 cut(s) 480
AoxI GGCC 2 cut(s) 252, 257
ApaLI GTGCAC 1 cut(s) 126
ApeKI GCWGC 2 cut(s) 15, 298
ApoI RAATTY 1 cut(s) 35
Asp700I GAANNNNTTC 1 cut(s) 376
AspLEI GCGC 2 cut(s) 83, 303
AsuHPI GGTGA 4 cut(s) 41, 156, 255, 590
BaeGI GKGCMC 1 cut(s) 130
BaeI ACNNNNGTAYC 2 cut(s) 277, 310
BalI TGGCCA 1 cut(s) 254
Bbv12I GWGCWC 1 cut(s) 130
BbvI GCAGC 2 cut(s) 2, 310
BccI CCATC 5 cut(s) 140, 202, 268, 615, 664
BceAI ACGGC 1 cut(s) 322
BciT130I CCWGG 2 cut(s) 256, 395
BcoDI GTCTC 2 cut(s) 230, 452
BfaI CTAG 2 cut(s) 318, 476
BisI GCNGC 2 cut(s) 16, 299
BlsI GCNGC 2 cut(s) 17, 300
Bme1390I CCNGG 2 cut(s) 256, 395
BmrFI CCNGG 2 cut(s) 256, 395
BmsI GCATC 3 cut(s) 262, 343, 488
BsaBI GATNNNNATC 1 cut(s) 276
BsaI GGTCTC 1 cut(s) 452
BsaJI CCNNGG 3 cut(s) 172, 393, 394
BsaXI ACNNNNNCTCC 2 cut(s) 659, 689
Bsc4I CCNNNNNNNGG 4 cut(s) 173, 282, 400, 659
Bse1I ACTGG 2 cut(s) 65, 208
Bse3DI GCAATG 1 cut(s) 76
Bse8I GATNNNNATC 1 cut(s) 276
BseBI CCWGG 2 cut(s) 256, 395
BseDI CCNNGG 3 cut(s) 172, 393, 394
BseJI GATNNNNATC 1 cut(s) 276
BseLI CCNNNNNNNGG 4 cut(s) 173, 282, 400, 659
BseMI GCAATG 1 cut(s) 76
BseMII CTCAG 3 cut(s) 103, 261, 477
BseNI ACTGG 2 cut(s) 65, 208
BseRI GAGGAG 1 cut(s) 217
BseSI GKGCMC 1 cut(s) 130
BseXI GCAGC 2 cut(s) 2, 310
BsgI GTGCAG 1 cut(s) 530
Bsh1236I CGCG 2 cut(s) 83, 303
BshFI GGCC 2 cut(s) 254, 259
BsiHKAI GWGCWC 1 cut(s) 130
BslFI GGGAC 4 cut(s) 143, 176, 376, 397
BslI CCNNNNNNNGG 4 cut(s) 173, 282, 400, 659
BsmAI GTCTC 2 cut(s) 230, 452
BsmFI GGGAC 4 cut(s) 143, 176, 376, 397
BsnI GGCC 2 cut(s) 254, 259
Bso31I GGTCTC 1 cut(s) 452
Bsp1286I GDGCHC 1 cut(s) 130
Bsp1407I TGTACA 1 cut(s) 69
Bsp143I GATC 4 cut(s) 152, 277, 312, 472
BspACI CCGC 1 cut(s) 117
BspANI GGCC 2 cut(s) 254, 259
BspCNI CTCAG 3 cut(s) 102, 260, 476
BspFNI CGCG 2 cut(s) 83, 303
BspPI GGATC 1 cut(s) 480
BspQI GCTCTTC 1 cut(s) 57
BspTNI GGTCTC 1 cut(s) 452
BsrDI GCAATG 1 cut(s) 76
BsrGI TGTACA 1 cut(s) 69
BsrI ACTGG 2 cut(s) 65, 208
BssECI CCNNGG 3 cut(s) 172, 393, 394
BssMI GATC 4 cut(s) 152, 277, 312, 472
BssT1I CCWWGG 1 cut(s) 172
Bst2UI CCWGG 2 cut(s) 256, 395
Bst4CI ACNGT 2 cut(s) 132, 214
Bst6I CTCTTC 1 cut(s) 57
BstAUI TGTACA 1 cut(s) 69
BstC8I GCNNGC 1 cut(s) 85
BstDEI CTNAG 3 cut(s) 89, 247, 463
BstENI CCTNNNNNAGG 1 cut(s) 171
BstFNI CGCG 2 cut(s) 83, 303
BstHHI GCGC 2 cut(s) 83, 303
BstKTI GATC 4 cut(s) 155, 280, 315, 475
BstMAI GTCTC 2 cut(s) 230, 452
BstMBI GATC 4 cut(s) 152, 277, 312, 472
BstNI CCWGG 2 cut(s) 256, 395
BstNSI RCATGY 1 cut(s) 549
BstSCI CCNGG 2 cut(s) 254, 393
BstSLI GKGCMC 1 cut(s) 130
BstUI CGCG 2 cut(s) 83, 303
BstV1I GCAGC 2 cut(s) 2, 310
BstX2I RGATCY 1 cut(s) 472
BstYI RGATCY 1 cut(s) 472
BsuRI GGCC 2 cut(s) 254, 259
BtsIMutI CAGTG 2 cut(s) 72, 255
Cac8I GCNNGC 1 cut(s) 85
CfoI GCGC 2 cut(s) 83, 303
Csp6I GTAC 2 cut(s) 70, 286
CviAII CATG 2 cut(s) 24, 546
CviJI RGCY 9 cut(s) 109, 142, 179, 218, 254, 259, 309, 615, 687
CviKI_1 RGCY 9 cut(s) 109, 142, 179, 218, 254, 259, 309, 615, 687
CviQI GTAC 2 cut(s) 70, 286
DdeI CTNAG 3 cut(s) 89, 247, 463
DpnI GATC 4 cut(s) 154, 279, 314, 474
DpnII GATC 4 cut(s) 152, 277, 312, 472
EaeI YGGCCR 1 cut(s) 252
Eam1104I CTCTTC 1 cut(s) 57
EarI CTCTTC 1 cut(s) 57
Eco130I CCWWGG 1 cut(s) 172
Eco31I GGTCTC 1 cut(s) 452
Eco57I CTGAAG 2 cut(s) 450, 543
EcoNI CCTNNNNNAGG 1 cut(s) 171
EcoRII CCWGG 2 cut(s) 254, 393
EcoT14I CCWWGG 1 cut(s) 172
ErhI CCWWGG 1 cut(s) 172
FaeI CATG 2 cut(s) 27, 549
FaiI YATR 9 cut(s) 25, 48, 123, 493, 495, 507, 547, 561, 624
FaqI GGGAC 4 cut(s) 143, 176, 376, 397
FatI CATG 2 cut(s) 23, 545
FauI CCCGC 1 cut(s) 110
FauNDI CATATG 1 cut(s) 493
Fnu4HI GCNGC 2 cut(s) 16, 299
Fsp4HI GCNGC 2 cut(s) 16, 299
FspBI CTAG 2 cut(s) 318, 476
GlaI GCGC 2 cut(s) 82, 302
GluI GCNGC 2 cut(s) 16, 299
HaeIII GGCC 2 cut(s) 254, 259
HhaI GCGC 2 cut(s) 83, 303
Hin1II CATG 2 cut(s) 27, 549
Hin6I GCGC 2 cut(s) 81, 301
HinP1I GCGC 2 cut(s) 81, 301
HinfI GANTC 2 cut(s) 238, 389
HphI GGTGA 4 cut(s) 41, 156, 255, 590
Hpy166II GTNNAC 2 cut(s) 70, 128
Hpy188I TCNGA 5 cut(s) 92, 282, 457, 466, 523
Hpy188III TCNNGA 2 cut(s) 198, 551
Hpy8I GTNNAC 2 cut(s) 70, 128
Hpy99I CGWCG 1 cut(s) 308
HpyCH4III ACNGT 2 cut(s) 132, 214
HpyCH4IV ACGT 1 cut(s) 292
HpyCH4V TGCA 6 cut(s) 128, 298, 346, 356, 441, 511
HpyF3I CTNAG 3 cut(s) 89, 247, 463
HpySE526I ACGT 1 cut(s) 292
Hsp92II CATG 2 cut(s) 27, 549
HspAI GCGC 2 cut(s) 81, 301
Kzo9I GATC 4 cut(s) 152, 277, 312, 472
LguI GCTCTTC 1 cut(s) 57
Lsp1109I GCAGC 2 cut(s) 2, 310
LweI GCATC 3 cut(s) 262, 343, 488
MaeI CTAG 2 cut(s) 318, 476
MaeII ACGT 1 cut(s) 292
MaeIII GTNAC 1 cut(s) 162
MalI GATC 4 cut(s) 154, 279, 314, 474
MboI GATC 4 cut(s) 152, 277, 312, 472
MflI RGATCY 1 cut(s) 472
MhlI GDGCHC 1 cut(s) 130
MlsI TGGCCA 1 cut(s) 254
MluCI AATT 2 cut(s) 35, 242
MluNI TGGCCA 1 cut(s) 254
MlyI GAGTC 2 cut(s) 232, 398
MmeI TCCRAC 1 cut(s) 612
MnlI CCTC 4 cut(s) 98, 238, 276, 472
Mox20I TGGCCA 1 cut(s) 254
MroXI GAANNNNTTC 1 cut(s) 376
MscI TGGCCA 1 cut(s) 254
Msp20I TGGCCA 1 cut(s) 254
MspA1I CMGCKG 1 cut(s) 117
MspR9I CCNGG 2 cut(s) 256, 395
MvaI CCWGG 2 cut(s) 256, 395
MvnI CGCG 2 cut(s) 83, 303
NdeI CATATG 1 cut(s) 493
NdeII GATC 4 cut(s) 152, 277, 312, 472
NlaIII CATG 2 cut(s) 27, 549
NmuCI GTSAC 1 cut(s) 162
NspI RCATGY 1 cut(s) 549
PasI CCCWGGG 1 cut(s) 394
PciI ACATGT 1 cut(s) 545
PciSI GCTCTTC 1 cut(s) 57
PdmI GAANNNNTTC 1 cut(s) 376
PflFI GACNNNGTC 1 cut(s) 388
PflMI CCANNNNNTGG 1 cut(s) 659
PkrI GCNGC 2 cut(s) 17, 300
PleI GAGTC 2 cut(s) 232, 397
PpsI GAGTC 2 cut(s) 232, 397
PscI ACATGT 1 cut(s) 545
Psp6I CCWGG 2 cut(s) 254, 393
PspGI CCWGG 2 cut(s) 254, 393
PsuI RGATCY 1 cut(s) 472
PsyI GACNNNGTC 1 cut(s) 388
RsaI GTAC 2 cut(s) 71, 287
RsaNI GTAC 2 cut(s) 70, 286
SapI GCTCTTC 1 cut(s) 57
SatI GCNGC 2 cut(s) 16, 299
Sau3AI GATC 4 cut(s) 152, 277, 312, 472
SchI GAGTC 2 cut(s) 232, 398
ScrFI CCNGG 2 cut(s) 256, 395
SduI GDGCHC 1 cut(s) 130
SetI ASST 7 cut(s) 111, 169, 220, 287, 295, 451, 464
SfaNI GCATC 3 cut(s) 262, 343, 488
Sse9I AATT 2 cut(s) 35, 242
SsiI CCGC 1 cut(s) 117
SspMI CTAG 2 cut(s) 318, 476
StyD4I CCNGG 2 cut(s) 254, 393
StyI CCWWGG 1 cut(s) 172
TaaI ACNGT 2 cut(s) 132, 214
TaiI ACGT 1 cut(s) 295
TasI AATT 2 cut(s) 35, 242
TatI WGTACW 1 cut(s) 69
TscAI CASTG 2 cut(s) 72, 255
TseFI GTSAC 1 cut(s) 162
TseI GCWGC 2 cut(s) 15, 298
Tsp45I GTSAC 1 cut(s) 162
TspDTI ATGAA 1 cut(s) 48
TspGWI ACGGA 1 cut(s) 427
TspRI CASTG 2 cut(s) 72, 255
Tth111I GACNNNGTC 1 cut(s) 388
Van91I CCANNNNNTGG 1 cut(s) 659
VneI GTGCAC 1 cut(s) 126
XagI CCTNNNNNAGG 1 cut(s) 171
XapI RAATTY 1 cut(s) 35
XceI RCATGY 1 cut(s) 549
XcmI CCANNNNNNNNNTGG 1 cut(s) 608
XmnI GAANNNNTTC 1 cut(s) 376
XspI CTAG 2 cut(s) 318, 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.