MD09G1066300.v1.1

Protein RIK isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005118
Physical Location & Seq
Forward (+)
4516840 .. 4519346
2507 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1066300.v1.1.491

Sequence Viewer

Length: 747 bp
ATGACGGAGGAGAGTGGCGCTAGGGTTTCCTCCGACGTGCCCAGCGATGATTCACAAACAAGGCAAAGGAAGAAAAGAAAGTGGGATCAACCTGCAGCATCCTTGGTTTCAGCCGGTTTAGCAGTGCCTGGGGCTCTCCCAATGGTAAATGTGGGATTTCTTGGTGGGATTACAATTCCTGGCACAACTCTGGTTACTGGTGCTGTTTCAATGAATCCATTTGTGGCCAGCTATGCTACCATACCTCAGCCGTATCAGGTTCCTCTGATCCCGCCACAGACTGCCACGGTCATTCAGAAATTAATTCAACCGAAGATTCAAGATGAGTTAATAATAGCACGAGAAATTGTTATTAACGATGCAGAGTCTTCAGTTCGCTACAAGTTGACAAAACGCCAAACACAGGAGGAGATCCAAAAATGCACGGGAGCTGTGGTGATAACAAGGGGAAAGTATCATCCACCAAATTCATCAACTGATGGTGAAAAGCCACTATATCTTCATATATCTTCAGGGGCTCATTTAAAAGAGACAGCAGAACGTATTTTAGCAGTTGACCGTGCAGCTGCCATGGTTGAAGAAATGTTGAAACAGGGTCAGAATCCAACTATGAGCAATGGAGTGATGGCACAAGGTACTTGTGTATATTTGGGCTTTGACGCAGATCCATCATTGAACATTGCAGCTCGTATACGTGGTCCAAATGTAAGTTATTTTGTTCCTCCCCTTTTCACGTCTTATCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

249

Amino Acids

26.81

Weight (kDa)

8.48

Isoelectric Point (pI)

40.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KH_12 PF23469 112 - 200 2.6e-36 KH-domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 100
AccI GTMKAC 1 cut(s) 691
AciI CCGC 1 cut(s) 272
AclWI GGATC 4 cut(s) 93, 262, 406, 659
AcoI YGGCCR 1 cut(s) 225
AcsI RAATTY 1 cut(s) 466
AcuI CTGAAG 2 cut(s) 354, 495
AfaI GTAC 1 cut(s) 637
AfiI CCNNNNNNNGG 1 cut(s) 403
AgsI TTSAA 6 cut(s) 210, 308, 320, 578, 589, 676
AjiI CACGTC 2 cut(s) 37, 735
AjnI CCWGG 2 cut(s) 127, 178
AluBI AGCT 4 cut(s) 231, 431, 566, 686
AluI AGCT 4 cut(s) 231, 431, 566, 686
Alw26I GTCTC 1 cut(s) 524
AlwI GGATC 4 cut(s) 93, 262, 406, 659
AlwNI CAGNNNCTG 1 cut(s) 128
AoxI GGCC 1 cut(s) 225
ApeKI GCWGC 4 cut(s) 95, 563, 566, 683
ApoI RAATTY 1 cut(s) 466
AseI ATTAAT 1 cut(s) 302
AspLEI GCGC 1 cut(s) 20
AspS9I GGNCC 1 cut(s) 698
AsuHPI GGTGA 2 cut(s) 448, 494
AvaII GGWCC 1 cut(s) 698
BaeGI GKGCMC 1 cut(s) 42
BaeI ACNNNNGTAYC 2 cut(s) 627, 660
BalI TGGCCA 1 cut(s) 227
BanII GRGCYC 2 cut(s) 136, 520
BauI CACGAG 1 cut(s) 339
BbsI GAAGAC 1 cut(s) 360
BbvCI CCTCAGC 1 cut(s) 246
BbvI GCAGC 4 cut(s) 107, 553, 575, 695
BccI CCATC 3 cut(s) 473, 619, 676
BceAI ACGGC 1 cut(s) 235
BciT130I CCWGG 2 cut(s) 129, 180
BcoDI GTCTC 1 cut(s) 524
BfaI CTAG 1 cut(s) 21
BfmI CTRYAG 1 cut(s) 93
BfoI RGCGCY 1 cut(s) 21
BfuAI ACCTGC 1 cut(s) 100
BisI GCNGC 4 cut(s) 96, 564, 567, 684
BlsI GCNGC 4 cut(s) 97, 565, 568, 685
Bme1390I CCNGG 2 cut(s) 129, 180
Bme18I GGWCC 1 cut(s) 698
BmgBI CACGTC 2 cut(s) 37, 735
BmgT120I GGNCC 1 cut(s) 698
BmiI GGNNCC 1 cut(s) 261
BmrFI CCNGG 2 cut(s) 129, 180
BmsI GCATC 2 cut(s) 107, 349
BpiI GAAGAC 1 cut(s) 360
Bpu10I CCTNAGC 1 cut(s) 246
BsaAI YACGTR 1 cut(s) 695
BsaJI CCNNGG 4 cut(s) 102, 128, 285, 570
BsaXI ACNNNNNCTCC 2 cut(s) 420, 450
Bsc4I CCNNNNNNNGG 1 cut(s) 403
Bse118I RCCGGY 1 cut(s) 113
Bse1I ACTGG 1 cut(s) 202
Bse3DI GCAATG 2 cut(s) 622, 678
BseBI CCWGG 2 cut(s) 129, 180
BseDI CCNNGG 4 cut(s) 102, 128, 285, 570
BseGI GGATG 2 cut(s) 98, 457
BseLI CCNNNNNNNGG 1 cut(s) 403
BseMI GCAATG 2 cut(s) 622, 678
BseMII CTCAG 1 cut(s) 260
BseNI ACTGG 1 cut(s) 202
BseRI GAGGAG 2 cut(s) 23, 422
BseSI GKGCMC 1 cut(s) 42
BseXI GCAGC 4 cut(s) 107, 553, 575, 695
BseYI CCCAGC 1 cut(s) 41
BsgI GTGCAG 1 cut(s) 582
BshFI GGCC 1 cut(s) 227
BsiSI CCGG 1 cut(s) 114
BslI CCNNNNNNNGG 1 cut(s) 403
BsmAI GTCTC 1 cut(s) 524
BsnI GGCC 1 cut(s) 227
Bsp1286I GDGCHC 3 cut(s) 42, 136, 520
Bsp143I GATC 4 cut(s) 85, 267, 411, 664
Bsp19I CCATGG 1 cut(s) 570
BspACI CCGC 1 cut(s) 272
BspANI GGCC 1 cut(s) 227
BspCNI CTCAG 1 cut(s) 259
BspLI GGNNCC 1 cut(s) 261
BspMAI CTGCAG 1 cut(s) 97
BspMI ACCTGC 1 cut(s) 100
BspPI GGATC 4 cut(s) 93, 262, 406, 659
BsrDI GCAATG 2 cut(s) 622, 678
BsrFI RCCGGY 1 cut(s) 113
BsrI ACTGG 1 cut(s) 202
BssAI RCCGGY 1 cut(s) 113
BssECI CCNNGG 4 cut(s) 102, 128, 285, 570
BssMI GATC 4 cut(s) 85, 267, 411, 664
BssNAI GTATAC 1 cut(s) 692
BssSI CACGAG 1 cut(s) 339
BssT1I CCWWGG 2 cut(s) 102, 570
Bst1107I GTATAC 1 cut(s) 692
Bst2BI CACGAG 1 cut(s) 339
Bst2UI CCWGG 2 cut(s) 129, 180
Bst4CI ACNGT 2 cut(s) 289, 560
BstBAI YACGTR 1 cut(s) 695
BstC8I GCNNGC 1 cut(s) 229
BstDEI CTNAG 1 cut(s) 246
BstDSI CCRYGG 2 cut(s) 285, 570
BstF5I GGATG 2 cut(s) 98, 457
BstH2I RGCGCY 1 cut(s) 21
BstHHI GCGC 1 cut(s) 20
BstKTI GATC 4 cut(s) 88, 270, 414, 667
BstMAI GTCTC 1 cut(s) 524
BstMBI GATC 4 cut(s) 85, 267, 411, 664
BstMWI GCNNNNNNNGC 2 cut(s) 119, 233
BstNI CCWGG 2 cut(s) 129, 180
BstSCI CCNGG 2 cut(s) 127, 178
BstSFI CTRYAG 1 cut(s) 93
BstSLI GKGCMC 1 cut(s) 42
BstV1I GCAGC 4 cut(s) 107, 553, 575, 695
BstV2I GAAGAC 1 cut(s) 360
BstX2I RGATCY 2 cut(s) 411, 664
BstYI RGATCY 2 cut(s) 411, 664
BstZ17I GTATAC 1 cut(s) 692
BsuRI GGCC 1 cut(s) 227
BtgI CCRYGG 2 cut(s) 285, 570
BtgZI GCGATG 1 cut(s) 60
BtrI CACGTC 2 cut(s) 37, 735
BtsCI GGATG 2 cut(s) 98, 457
BtsI GCAGTG 1 cut(s) 129
BtsIMutI CAGTG 1 cut(s) 129
BveI ACCTGC 1 cut(s) 100
Cac8I GCNNGC 1 cut(s) 229
CaiI CAGNNNCTG 1 cut(s) 128
CfoI GCGC 1 cut(s) 20
Cfr10I RCCGGY 1 cut(s) 113
Cfr13I GGNCC 1 cut(s) 698
CseI GACGC 1 cut(s) 668
Csp6I GTAC 1 cut(s) 636
CviAII CATG 1 cut(s) 571
CviQI GTAC 1 cut(s) 636
DdeI CTNAG 1 cut(s) 246
DpnI GATC 4 cut(s) 87, 269, 413, 666
DpnII GATC 4 cut(s) 85, 267, 411, 664
DraI TTTAAA 1 cut(s) 525
EaeI YGGCCR 1 cut(s) 225
Eco130I CCWWGG 2 cut(s) 102, 570
Eco24I GRGCYC 2 cut(s) 136, 520
Eco47I GGWCC 1 cut(s) 698
Eco57I CTGAAG 2 cut(s) 354, 495
EcoRII CCWGG 2 cut(s) 127, 178
EcoT14I CCWWGG 2 cut(s) 102, 570
EcoT38I GRGCYC 2 cut(s) 136, 520
ErhI CCWWGG 2 cut(s) 102, 570
FaeI CATG 1 cut(s) 574
FaiI YATR 9 cut(s) 234, 242, 496, 504, 506, 572, 611, 646, 692
FatI CATG 1 cut(s) 570
FauI CCCGC 1 cut(s) 279
FblI GTMKAC 1 cut(s) 691
Fnu4HI GCNGC 4 cut(s) 96, 564, 567, 684
FokI GGATG 2 cut(s) 85, 444
FriOI GRGCYC 2 cut(s) 136, 520
Fsp4HI GCNGC 4 cut(s) 96, 564, 567, 684
FspBI CTAG 1 cut(s) 21
GlaI GCGC 1 cut(s) 19
GluI GCNGC 4 cut(s) 96, 564, 567, 684
GsaI CCCAGC 1 cut(s) 45
HaeII RGCGCY 1 cut(s) 21
HaeIII GGCC 1 cut(s) 227
HapII CCGG 1 cut(s) 114
HgaI GACGC 1 cut(s) 668
HhaI GCGC 1 cut(s) 20
Hin1II CATG 1 cut(s) 574
Hin6I GCGC 1 cut(s) 18
HinP1I GCGC 1 cut(s) 18
HincII GTYRAC 2 cut(s) 387, 556
HindII GTYRAC 2 cut(s) 387, 556
HinfI GANTC 5 cut(s) 50, 214, 316, 365, 601
HpaII CCGG 1 cut(s) 114
HphI GGTGA 2 cut(s) 448, 494
Hpy166II GTNNAC 3 cut(s) 387, 556, 692
Hpy188I TCNGA 4 cut(s) 34, 267, 297, 600
Hpy188III TCNNGA 1 cut(s) 320
Hpy8I GTNNAC 3 cut(s) 387, 556, 692
Hpy99I CGWCG 1 cut(s) 38
HpyCH4III ACNGT 2 cut(s) 289, 560
HpyCH4IV ACGT 4 cut(s) 36, 541, 694, 734
HpyCH4V TGCA 5 cut(s) 95, 362, 423, 563, 683
HpyF10VI GCNNNNNNNGC 2 cut(s) 119, 233
HpyF3I CTNAG 1 cut(s) 246
HpySE526I ACGT 4 cut(s) 36, 541, 694, 734
Hsp92II CATG 1 cut(s) 574
HspAI GCGC 1 cut(s) 18
Kzo9I GATC 4 cut(s) 85, 267, 411, 664
LmnI GCTCC 1 cut(s) 428
Lsp1109I GCAGC 4 cut(s) 107, 553, 575, 695
LweI GCATC 2 cut(s) 107, 349
MaeI CTAG 1 cut(s) 21
MaeII ACGT 4 cut(s) 36, 541, 694, 734
MaeIII GTNAC 1 cut(s) 193
MalI GATC 4 cut(s) 87, 269, 413, 666
MboI GATC 4 cut(s) 85, 267, 411, 664
MboII GAAGA 6 cut(s) 82, 325, 360, 491, 501, 590
MflI RGATCY 2 cut(s) 411, 664
MhlI GDGCHC 3 cut(s) 42, 136, 520
MlsI TGGCCA 1 cut(s) 227
MluCI AATT 5 cut(s) 174, 299, 303, 345, 466
MluNI TGGCCA 1 cut(s) 227
MlyI GAGTC 1 cut(s) 374
MmeI TCCRAC 2 cut(s) 57, 629
MnlI CCTC 5 cut(s) 40, 255, 273, 400, 732
Mox20I TGGCCA 1 cut(s) 227
MscI TGGCCA 1 cut(s) 227
MseI TTAA 4 cut(s) 302, 329, 354, 524
Msp20I TGGCCA 1 cut(s) 227
MspA1I CMGCKG 1 cut(s) 566
MspI CCGG 1 cut(s) 114
MspR9I CCNGG 2 cut(s) 129, 180
MvaI CCWGG 2 cut(s) 129, 180
MwoI GCNNNNNNNGC 2 cut(s) 119, 233
NcoI CCATGG 1 cut(s) 570
NdeII GATC 4 cut(s) 85, 267, 411, 664
NlaIII CATG 1 cut(s) 574
NlaIV GGNNCC 1 cut(s) 261
PcsI WCGNNNNNNNCGW 1 cut(s) 42
PfeI GAWTC 4 cut(s) 50, 214, 316, 601
PkrI GCNGC 4 cut(s) 97, 565, 568, 685
PleI GAGTC 1 cut(s) 373
PpsI GAGTC 1 cut(s) 373
Ppu21I YACGTR 1 cut(s) 695
PshBI ATTAAT 1 cut(s) 302
Psp6I CCWGG 2 cut(s) 127, 178
PspFI CCCAGC 1 cut(s) 41
PspGI CCWGG 2 cut(s) 127, 178
PspN4I GGNNCC 1 cut(s) 261
PspPI GGNCC 1 cut(s) 698
PstI CTGCAG 1 cut(s) 97
PstNI CAGNNNCTG 1 cut(s) 128
PsuI RGATCY 2 cut(s) 411, 664
PvuII CAGCTG 1 cut(s) 566
RsaI GTAC 1 cut(s) 637
RsaNI GTAC 1 cut(s) 636
SaqAI TTAA 4 cut(s) 302, 329, 354, 524
SatI GCNGC 4 cut(s) 96, 564, 567, 684
Sau3AI GATC 4 cut(s) 85, 267, 411, 664
Sau96I GGNCC 1 cut(s) 698
SchI GAGTC 1 cut(s) 374
ScrFI CCNGG 2 cut(s) 129, 180
SduI GDGCHC 3 cut(s) 42, 136, 520
SfaNI GCATC 2 cut(s) 107, 349
SfcI CTRYAG 1 cut(s) 93
SinI GGWCC 1 cut(s) 698
Sse9I AATT 5 cut(s) 174, 299, 303, 345, 466
SsiI CCGC 1 cut(s) 272
SspMI CTAG 1 cut(s) 21
StyD4I CCNGG 2 cut(s) 127, 178
StyI CCWWGG 2 cut(s) 102, 570
TaaI ACNGT 2 cut(s) 289, 560
TaiI ACGT 4 cut(s) 39, 544, 697, 737
TasI AATT 5 cut(s) 174, 299, 303, 345, 466
TfiI GAWTC 4 cut(s) 50, 214, 316, 601
Tru1I TTAA 4 cut(s) 302, 329, 354, 524
Tru9I TTAA 4 cut(s) 302, 329, 354, 524
TscAI CASTG 1 cut(s) 129
TseI GCWGC 4 cut(s) 95, 563, 566, 683
TspDTI ATGAA 3 cut(s) 227, 459, 491
TspGWI ACGGA 1 cut(s) 20
TspRI CASTG 1 cut(s) 129
VpaK11BI GGWCC 1 cut(s) 698
VspI ATTAAT 1 cut(s) 302
XapI RAATTY 1 cut(s) 466
XmiI GTMKAC 1 cut(s) 691
XspI CTAG 1 cut(s) 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.