Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0005118

Physical Location & Seq

Genomic Coordinates Forward (+)

7728087 .. 7734105

Gene Structure 6019 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00085390.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1881 bp

ATGACGGAGGAGAGCAGCGCTAGAGTTTCCAATGATGCATCGCAAACAAGGCAAAGAAAGAAAAGAAAATGGGATCAACCGGCGGAGTCATTGGTTTCAGCTGGTTTAGCGGTGCCTGGGGCGCTCCCATTAGGCAATGTGGGATTGCTTGGTGGGATGACGCTTCCCGGTGTAAGTCCAGTGTCCGGTGCGATCAATCCACTTGCTGCTGGCTTTCCTACTGTACCGCAGGCGTATCAGGTGCCTCTGATACCGCAGCAAGCTGCAACAGTTATCCAGAAACTAATACAACCAAAGATCCAAGATGAGTTAATAGCACGAGAAATTGTTATTAATGATGCAGAGTCTTCTATTCGGTACAAGCTGACAAAACGCCAAACACAGGAGGAGATTCAAAGGTGCACTGGTGCTGTGGTGATAACAAGGGGTAAGTATCATCCACCAAATGCGTCAACTGATGGTGAAAAGCCATTATATCTTCATATTTCTGCAGGGGCTAATATAAAAGAGACGACAGAACGGATTTTAGCAGTTGATCATGCTGCTGCCATGGTTGAAGAAATGTTGAAACAGGGTCAAAATTTACATCCCTTGTCATTGTCGTCTCTTTCTACCGTGAGTAATGGACTGAAGGAACCAAGCACATGTGTATATCTGGGCTTTGATGCAGATCCATCATTGAACATTGCTGCCCGTATACGTGGACCAAGTGACCAGTATATAAATCACATTATGAATGAAACAGGAGCAACTGTCTTACTCAGAGGGCGTGGTTCAGGAAATATTGATTGTTTACATGGTGAAGAAGGACAACAACCATTGCATCTGTTCTTGTCAAGTAATAATTTGAAAAGTCTCGAAGATGCTAGGCTTTTGGCTGAACATCTTTTGGATACAATCAGCGTAGAGTGTGGTGTCTCCAGGGTTTCTTCATCTAAGGTTTATAGTGCTATTGCACCCCCACAGCAGGTATATAGTGCTGTTCCTCCACCACATCAGTTGTCAGCTGGAGTTCAGAGTTCTGGAAATGAGGTAAAAGCCATTACAACTTTGGCGTCTGCAACTGTTGGTCTTACACCAGCTCCCCCGGTTTCCTCAGTTGGGACTCCTGTGACCACTACCGTTTTTACTCAAGGGACGGTATCTCAGTCTGGAGGATTTTCAAACTGTGTGCAATCTCAGGCAAGCAGTGGTGGTTGTCCACAACTTCCATCTGGTGGAACAATCTACAGTGGATATGGTGGGATATATCCTCAAGCCACACCTTTACAACAAGTTGCCCTGGCCCTTAGACAGTCAACATCCCCTATCACTTCTACAATTGCTCCCACAACATCAGTCCCAAGCACTGAACCACAGTTTAATACGAAATTCAGTTCTGATTCTGAGAAGGAAAAACGGCCTCCGCAAAGACGGAAGTTTCAGGAGTTACCAGTTAGTTCGAAGGGTCCTGCCAAAGTTCATCAGGTATTTTCTCTGACCTTCTATGAGGCTAACTGGCCGATACTGGACCTCTCTATTGGGCCATTGGAATTGGACTTATTAAAGCCACCTGAACTGGCGTCAGATTTGGGTGTGAGAAATGTATCAACTATGCCAGCTCCAAAGAAGTTGGTCCAGCAGGTTTCAAATGGAATGCCGCCACCACCACCCAGAACCATGCCTCCACCTCCACCAAAATTTACATCATCAACACAGGTTGTTAAAGAGATTGACAAGAACAATGTTCTGAAAAAGACAAAATCTGATAATGTACCTGATACTTTGGTCAAGCTCATGGAATATGGAGAAGATGATGATGACTCTGAGGAAACTGATGAACAGTCACCCAATAGCAACCCTGTTGCAGTAACAGCTCGAAAGCCTTTCTGGGCTTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0097159 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

626

Amino Acids

66.81

Weight (kDa)

6.4

Isoelectric Point (pI)

52.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
KH_12	PF23469	105 - 193	2e-35	KH-domain
KH-I_KHDC4-BBP	PF22675	223 - 298	9.5e-26	KHDC4/BBP-like, KH-domain type I

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005118)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G29390 AT3G29390
fragaria_vesca	FvH4_6g46910 FvH4_6g46910 FvH4_6g46910 FvH4_6g46910
malus_domestica	MD09G1066300.v1.1 MD09G1066400.v1.1
prunus_persica	Prupe.3G255200_v2.0.a1 Prupe.3G255200_v2.0.a1
pyrus_communis	pycom111g05570
rosa_chinensis	RchiOBHm_Chr2g0165881
rosa_laevigata	RLG00000021564
rosa_multiflora	Rmu_co8354311.1_g000001 Rmu_sc0001982.1_g000006 Rmu_sc0006218.1_g000001 Rmu_sc0006218.1_g000002 Rmu_sc0008709.1_g000009 Rmu_sc0013817.1_g000001
rosa_roxburghii	Rroxscaffold_2G00085390
rosa_rugosa	Rorug02G0518600 Rorug02G0518700 Rorug02G0518800 Rorug05G0064400 Rorug05G0462700
rosa_samantha	Rh2AG584500 Rh2BG596300 Rh2CG567200 Rh2DG606100
rosa_wichuraiana	Rw2G048730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	958, 1612
AccB1I	GGYRCC	2 cut(s)	112, 241
AccB7I	CCANNNNNTGG	1 cut(s)	1217
AccI	GTMKAC	1 cut(s)	697
AciI	CCGC	6 cut(s)	83, 110, 227, 254, 1406, 1640
AclWI	GGATC	3 cut(s)	81, 292, 665
AcoI	YGGCCR	1 cut(s)	1499
AcsI	RAATTY	3 cut(s)	580, 1370, 1679
AcuI	CTGAAG	1 cut(s)	650
AcyI	GRCGYC	2 cut(s)	1055, 1562
AfaI	GTAC	3 cut(s)	225, 359, 1755
AfeI	AGCGCT	1 cut(s)	19
AfiI	CCNNNNNNNGG	5 cut(s)	185, 382, 967, 1101, 1217
AflIII	ACRYGT	1 cut(s)	644
AgsI	TTSAA	7 cut(s)	395, 557, 568, 682, 850, 1164, 1629
AjnI	CCWGG	3 cut(s)	115, 920, 1281
AloI	GAACNNNNNNTCC	2 cut(s)	1648, 1680
AluBI	AGCT	8 cut(s)	101, 263, 364, 1007, 1082, 1601, 1774, 1856
AluI	AGCT	8 cut(s)	101, 263, 364, 1007, 1082, 1601, 1774, 1856
Alw21I	GWGCWC	1 cut(s)	404
Alw26I	GTCTC	4 cut(s)	503, 609, 860, 922
Alw44I	GTGCAC	1 cut(s)	400
AlwI	GGATC	3 cut(s)	81, 292, 665
Aor51HI	AGCGCT	1 cut(s)	19
AoxI	GGCC	4 cut(s)	1284, 1400, 1499, 1523
ApaLI	GTGCAC	1 cut(s)	400
ApeKI	GCWGC	7 cut(s)	15, 206, 256, 263, 542, 545, 689
ApoI	RAATTY	3 cut(s)	580, 1370, 1679
AseI	ATTAAT	1 cut(s)	333
AspLEI	GCGC	2 cut(s)	20, 124
AspS9I	GGNCC	6 cut(s)	704, 1285, 1448, 1510, 1523, 1615
AsuC2I	CCSGG	2 cut(s)	168, 1088
AsuHPI	GGTGA	4 cut(s)	427, 473, 812, 1818
AsuII	TTCGAA	1 cut(s)	1442
AvaII	GGWCC	4 cut(s)	704, 1448, 1510, 1615
BaeGI	GKGCMC	1 cut(s)	404
BaeI	ACNNNNGTAYC	2 cut(s)	1752, 1785
BanI	GGYRCC	2 cut(s)	112, 241
BauI	CACGAG	1 cut(s)	318
BbsI	GAAGAC	1 cut(s)	339
Bbv12I	GWGCWC	1 cut(s)	404
BbvI	GCAGC	7 cut(s)	27, 193, 250, 268, 529, 532, 676
BccI	CCATC	3 cut(s)	452, 682, 1219
BceAI	ACGGC	1 cut(s)	1415
BciT130I	CCWGG	3 cut(s)	117, 922, 1283
BciVI	GTATCC	1 cut(s)	886
BclI	TGATCA	1 cut(s)	535
BcnI	CCSGG	2 cut(s)	168, 1088
BcoDI	GTCTC	4 cut(s)	503, 609, 860, 922
BfaI	CTAG	2 cut(s)	21, 867
BfmI	CTRYAG	2 cut(s)	489, 1228
BfoI	RGCGCY	2 cut(s)	21, 125
BfuAI	ACCTGC	2 cut(s)	958, 1612
BfuI	GTATCC	1 cut(s)	886
BisI	GCNGC	8 cut(s)	16, 207, 257, 264, 543, 546, 690, 1640
BlsI	GCNGC	8 cut(s)	17, 208, 258, 265, 544, 547, 691, 1641
Bme1390I	CCNGG	5 cut(s)	117, 168, 922, 1088, 1283
Bme18I	GGWCC	4 cut(s)	704, 1448, 1510, 1615
BmgT120I	GGNCC	6 cut(s)	704, 1285, 1448, 1510, 1523, 1615
BmiI	GGNNCC	4 cut(s)	114, 243, 636, 1449
BmrFI	CCNGG	5 cut(s)	117, 168, 922, 1088, 1283
BmsI	GCATC	6 cut(s)	25, 47, 328, 655, 832, 853
BpiI	GAAGAC	1 cut(s)	339
BpmI	CTGGAG	3 cut(s)	904, 1029, 1173
Bpu14I	TTCGAA	1 cut(s)	1442
BpuEI	CTTGAG	2 cut(s)	1116, 1239
BpuMI	CCSGG	2 cut(s)	168, 1088
BsaAI	YACGTR	1 cut(s)	701
BsaBI	GATNNNNATC	1 cut(s)	669
BsaHI	GRCGYC	2 cut(s)	1055, 1562
BsaJI	CCNNGG	5 cut(s)	116, 549, 921, 1086, 1281
BsaWI	WCCGGW	1 cut(s)	185
BsaXI	ACNNNNNCTCC	8 cut(s)	738, 768, 1066, 1096, 1309, 1339, 1648, 1678
Bsc4I	CCNNNNNNNGG	5 cut(s)	185, 382, 967, 1101, 1217
Bse118I	RCCGGY	1 cut(s)	79
Bse1I	ACTGG	7 cut(s)	179, 409, 715, 1433, 1502, 1512, 1563
Bse3DI	GCAATG	3 cut(s)	142, 684, 818
Bse8I	GATNNNNATC	1 cut(s)	669
BseBI	CCWGG	3 cut(s)	117, 922, 1283
BseDI	CCNNGG	5 cut(s)	116, 549, 921, 1086, 1281
BseGI	GGATG	4 cut(s)	162, 436, 586, 1301
BseJI	GATNNNNATC	1 cut(s)	669
BseLI	CCNNNNNNNGG	5 cut(s)	185, 382, 967, 1101, 1217
BseMI	GCAATG	3 cut(s)	142, 684, 818
BseMII	CTCAG	6 cut(s)	775, 1110, 1160, 1193, 1377, 1797
BseNI	ACTGG	7 cut(s)	179, 409, 715, 1433, 1502, 1512, 1563
BseRI	GAGGAG	2 cut(s)	23, 401
BseSI	GKGCMC	1 cut(s)	404
BseXI	GCAGC	7 cut(s)	27, 193, 250, 268, 529, 532, 676
BshFI	GGCC	4 cut(s)	1286, 1402, 1501, 1525
BshNI	GGYRCC	2 cut(s)	112, 241
BsiHKAI	GWGCWC	1 cut(s)	404
BsiSI	CCGG	4 cut(s)	80, 168, 186, 1088
BslFI	GGGAC	3 cut(s)	1117, 1150, 1325
BslI	CCNNNNNNNGG	5 cut(s)	185, 382, 967, 1101, 1217
BsmAI	GTCTC	4 cut(s)	503, 609, 860, 922
BsmBI	CGTCTC	2 cut(s)	503, 609
BsmFI	GGGAC	3 cut(s)	1117, 1150, 1325
BsmI	GAATGC	1 cut(s)	1641
BsnI	GGCC	4 cut(s)	1286, 1402, 1501, 1525
Bsp119I	TTCGAA	1 cut(s)	1442
Bsp1286I	GDGCHC	1 cut(s)	404
Bsp143I	GATC	5 cut(s)	73, 192, 297, 535, 670
Bsp19I	CCATGG	1 cut(s)	549
BspACI	CCGC	6 cut(s)	83, 110, 227, 254, 1406, 1640
BspANI	GGCC	4 cut(s)	1286, 1402, 1501, 1525
BspCNI	CTCAG	6 cut(s)	774, 1109, 1159, 1192, 1378, 1798
BspLI	GGNNCC	4 cut(s)	114, 243, 636, 1449
BspMAI	CTGCAG	1 cut(s)	493
BspMI	ACCTGC	2 cut(s)	958, 1612
BspPI	GGATC	3 cut(s)	81, 292, 665
BspT104I	TTCGAA	1 cut(s)	1442
BspT107I	GGYRCC	2 cut(s)	112, 241
BsrDI	GCAATG	3 cut(s)	142, 684, 818
BsrFI	RCCGGY	1 cut(s)	79
BsrI	ACTGG	7 cut(s)	179, 409, 715, 1433, 1502, 1512, 1563
BssAI	RCCGGY	1 cut(s)	79
BssECI	CCNNGG	5 cut(s)	116, 549, 921, 1086, 1281
BssMI	GATC	5 cut(s)	73, 192, 297, 535, 670
BssNAI	GTATAC	1 cut(s)	698
BssNI	GRCGYC	2 cut(s)	1055, 1562
BssSI	CACGAG	1 cut(s)	318
BssT1I	CCWWGG	1 cut(s)	549
Bst1107I	GTATAC	1 cut(s)	698
Bst2BI	CACGAG	1 cut(s)	318
Bst2UI	CCWGG	3 cut(s)	117, 922, 1283
BstACI	GRCGYC	2 cut(s)	1055, 1562
BstBAI	YACGTR	1 cut(s)	701
BstBI	TTCGAA	1 cut(s)	1442
BstC8I	GCNNGC	5 cut(s)	211, 231, 261, 1186, 1599
BstDEI	CTNAG	8 cut(s)	761, 936, 1096, 1146, 1179, 1289, 1386, 1806
BstDSI	CCRYGG	1 cut(s)	549
BstF5I	GGATG	4 cut(s)	162, 436, 586, 1301
BstH2I	RGCGCY	2 cut(s)	21, 125
BstHHI	GCGC	2 cut(s)	20, 124
BstKTI	GATC	5 cut(s)	76, 195, 300, 538, 673
BstMAI	GTCTC	4 cut(s)	503, 609, 860, 922
BstMBI	GATC	5 cut(s)	73, 192, 297, 535, 670
BstMWI	GCNNNNNNNGC	4 cut(s)	49, 107, 121, 1853
BstNI	CCWGG	3 cut(s)	117, 922, 1283
BstNSI	RCATGY	1 cut(s)	648
BstSCI	CCNGG	5 cut(s)	115, 166, 920, 1086, 1281
BstSFI	CTRYAG	2 cut(s)	489, 1228
BstSLI	GKGCMC	1 cut(s)	404
BstV1I	GCAGC	7 cut(s)	27, 193, 250, 268, 529, 532, 676
BstV2I	GAAGAC	1 cut(s)	339
BstX2I	RGATCY	2 cut(s)	297, 670
BstYI	RGATCY	2 cut(s)	297, 670
BstZ17I	GTATAC	1 cut(s)	698
BsuI	GTATCC	1 cut(s)	886
BsuRI	GGCC	4 cut(s)	1286, 1402, 1501, 1525
BtgI	CCRYGG	1 cut(s)	549
BtgZI	GCGATG	1 cut(s)	24
BtsCI	GGATG	4 cut(s)	162, 436, 586, 1301
BtsI	GCAGTG	1 cut(s)	1195
BtsIMutI	CAGTG	5 cut(s)	186, 402, 1195, 1237, 1347
BveI	ACCTGC	2 cut(s)	958, 1612
Cac8I	GCNNGC	5 cut(s)	211, 231, 261, 1186, 1599
CfoI	GCGC	2 cut(s)	20, 124
Cfr10I	RCCGGY	1 cut(s)	79
Cfr13I	GGNCC	6 cut(s)	704, 1285, 1448, 1510, 1523, 1615
CseI	GACGC	4 cut(s)	169, 438, 1044, 1551
Csp6I	GTAC	3 cut(s)	224, 358, 1754
CspCI	CAANNNNNGTGG	2 cut(s)	981, 1016
CviAII	CATG	6 cut(s)	539, 550, 645, 797, 1660, 1777
CviQI	GTAC	3 cut(s)	224, 358, 1754
DdeI	CTNAG	8 cut(s)	761, 936, 1096, 1146, 1179, 1289, 1386, 1806
DpnI	GATC	5 cut(s)	75, 194, 299, 537, 672
DpnII	GATC	5 cut(s)	73, 192, 297, 535, 670
EaeI	YGGCCR	1 cut(s)	1499
EciI	GGCGGA	1 cut(s)	98
Eco130I	CCWWGG	1 cut(s)	549
Eco47I	GGWCC	4 cut(s)	704, 1448, 1510, 1615
Eco47III	AGCGCT	1 cut(s)	19
Eco57I	CTGAAG	1 cut(s)	650
EcoO109I	RGGNCCY	1 cut(s)	1448
EcoRII	CCWGG	3 cut(s)	115, 920, 1281
EcoT14I	CCWWGG	1 cut(s)	549
EcoT22I	ATGCAT	1 cut(s)	40
ErhI	CCWWGG	1 cut(s)	549
Esp3I	CGTCTC	2 cut(s)	503, 609
FaeI	CATG	6 cut(s)	542, 553, 648, 800, 1663, 1780
FaqI	GGGAC	3 cut(s)	1117, 1150, 1325
FatI	CATG	6 cut(s)	538, 549, 644, 796, 1659, 1776
FbaI	TGATCA	1 cut(s)	535
FblI	GTMKAC	1 cut(s)	697
Fnu4HI	GCNGC	8 cut(s)	16, 207, 257, 264, 543, 546, 690, 1640
FokI	GGATG	4 cut(s)	169, 423, 573, 1288
Fsp4HI	GCNGC	8 cut(s)	16, 207, 257, 264, 543, 546, 690, 1640
FspBI	CTAG	2 cut(s)	21, 867
GlaI	GCGC	2 cut(s)	19, 123
GluI	GCNGC	8 cut(s)	16, 207, 257, 264, 543, 546, 690, 1640
GsuI	CTGGAG	3 cut(s)	904, 1029, 1173
HaeII	RGCGCY	2 cut(s)	21, 125
HaeIII	GGCC	4 cut(s)	1286, 1402, 1501, 1525
HapII	CCGG	4 cut(s)	80, 168, 186, 1088
HgaI	GACGC	4 cut(s)	169, 438, 1044, 1551
HhaI	GCGC	2 cut(s)	20, 124
Hin1I	GRCGYC	2 cut(s)	1055, 1562
Hin1II	CATG	6 cut(s)	542, 553, 648, 800, 1663, 1780
Hin6I	GCGC	2 cut(s)	18, 122
HinP1I	GCGC	2 cut(s)	18, 122
HincII	GTYRAC	2 cut(s)	453, 1299
HindII	GTYRAC	2 cut(s)	453, 1299
HinfI	GANTC	6 cut(s)	86, 344, 391, 1105, 1382, 1802
HpaII	CCGG	4 cut(s)	80, 168, 186, 1088
HphI	GGTGA	4 cut(s)	427, 473, 812, 1818
Hpy166II	GTNNAC	7 cut(s)	402, 453, 698, 704, 794, 1202, 1299
Hpy188III	TCNNGA	6 cut(s)	277, 777, 857, 1023, 1152, 1424
Hpy8I	GTNNAC	7 cut(s)	402, 453, 698, 704, 794, 1202, 1299
HpyAV	CCTTC	5 cut(s)	625, 800, 1384, 1438, 1492
HpyCH4IV	ACGT	1 cut(s)	700
HpyF10VI	GCNNNNNNNGC	4 cut(s)	49, 107, 121, 1853
HpyF3I	CTNAG	8 cut(s)	761, 936, 1096, 1146, 1179, 1289, 1386, 1806
HpySE526I	ACGT	1 cut(s)	700
Hsp92I	GRCGYC	2 cut(s)	1055, 1562
Hsp92II	CATG	6 cut(s)	542, 553, 648, 800, 1663, 1780
HspAI	GCGC	2 cut(s)	18, 122
Ksp22I	TGATCA	1 cut(s)	535
Kzo9I	GATC	5 cut(s)	73, 192, 297, 535, 670
LmnI	GCTCC	5 cut(s)	129, 746, 1087, 1330, 1606
Lsp1109I	GCAGC	7 cut(s)	27, 193, 250, 268, 529, 532, 676
LweI	GCATC	6 cut(s)	25, 47, 328, 655, 832, 853
MaeI	CTAG	2 cut(s)	21, 867
MaeII	ACGT	1 cut(s)	700
MaeIII	GTNAC	5 cut(s)	710, 1111, 1428, 1824, 1849
MalI	GATC	5 cut(s)	75, 194, 299, 537, 672
MboI	GATC	5 cut(s)	73, 192, 297, 535, 670
MboII	GAAGA	7 cut(s)	339, 470, 569, 815, 872, 921, 1802
MfeI	CAATTG	1 cut(s)	1320
MflI	RGATCY	2 cut(s)	297, 670
MhlI	GDGCHC	1 cut(s)	404
MluCI	AATT	7 cut(s)	324, 580, 844, 1320, 1370, 1532, 1679
MlyI	GAGTC	4 cut(s)	95, 353, 1099, 1796
Mph1103I	ATGCAT	1 cut(s)	40
MseI	TTAA	5 cut(s)	311, 333, 1362, 1544, 1704
MspA1I	CMGCKG	2 cut(s)	101, 1007
MspI	CCGG	4 cut(s)	80, 168, 186, 1088
MspR9I	CCNGG	5 cut(s)	117, 168, 922, 1088, 1283
MunI	CAATTG	1 cut(s)	1320
Mva1269I	GAATGC	1 cut(s)	1641
MvaI	CCWGG	3 cut(s)	117, 922, 1283
MwoI	GCNNNNNNNGC	4 cut(s)	49, 107, 121, 1853
NciI	CCSGG	2 cut(s)	168, 1088
NcoI	CCATGG	1 cut(s)	549
NdeII	GATC	5 cut(s)	73, 192, 297, 535, 670
NlaIII	CATG	6 cut(s)	542, 553, 648, 800, 1663, 1780
NlaIV	GGNNCC	4 cut(s)	114, 243, 636, 1449
NmuCI	GTSAC	3 cut(s)	710, 1111, 1824
NsiI	ATGCAT	1 cut(s)	40
NspI	RCATGY	1 cut(s)	648
NspV	TTCGAA	1 cut(s)	1442
PciI	ACATGT	1 cut(s)	644
PctI	GAATGC	1 cut(s)	1641
PfeI	GAWTC	2 cut(s)	391, 1382
PflMI	CCANNNNNTGG	1 cut(s)	1217
PkrI	GCNGC	8 cut(s)	17, 208, 258, 265, 544, 547, 691, 1641
PleI	GAGTC	4 cut(s)	94, 352, 1099, 1796
PpsI	GAGTC	4 cut(s)	94, 352, 1099, 1796
Ppu21I	YACGTR	1 cut(s)	701
PpuMI	RGGWCCY	1 cut(s)	1448
PscI	ACATGT	1 cut(s)	644
PshBI	ATTAAT	1 cut(s)	333
Psp5II	RGGWCCY	1 cut(s)	1448
Psp6I	CCWGG	3 cut(s)	115, 920, 1281
PspGI	CCWGG	3 cut(s)	115, 920, 1281
PspN4I	GGNNCC	4 cut(s)	114, 243, 636, 1449
PspPI	GGNCC	6 cut(s)	704, 1285, 1448, 1510, 1523, 1615
PspPPI	RGGWCCY	1 cut(s)	1448
PstI	CTGCAG	1 cut(s)	493
PsuI	RGATCY	2 cut(s)	297, 670
PvuII	CAGCTG	2 cut(s)	101, 1007
RsaI	GTAC	3 cut(s)	225, 359, 1755
RsaNI	GTAC	3 cut(s)	224, 358, 1754
SaqAI	TTAA	5 cut(s)	311, 333, 1362, 1544, 1704
SatI	GCNGC	8 cut(s)	16, 207, 257, 264, 543, 546, 690, 1640
Sau3AI	GATC	5 cut(s)	73, 192, 297, 535, 670
Sau96I	GGNCC	6 cut(s)	704, 1285, 1448, 1510, 1523, 1615
SchI	GAGTC	4 cut(s)	95, 353, 1099, 1796
ScrFI	CCNGG	5 cut(s)	117, 168, 922, 1088, 1283
SduI	GDGCHC	1 cut(s)	404
SfaNI	GCATC	6 cut(s)	25, 47, 328, 655, 832, 853
SfcI	CTRYAG	2 cut(s)	489, 1228
SfuI	TTCGAA	1 cut(s)	1442
SinI	GGWCC	4 cut(s)	704, 1448, 1510, 1615
SmlI	CTYRAG	2 cut(s)	1131, 1254
SmoI	CTYRAG	2 cut(s)	1131, 1254
Sse9I	AATT	7 cut(s)	324, 580, 844, 1320, 1370, 1532, 1679
SsiI	CCGC	6 cut(s)	83, 110, 227, 254, 1406, 1640
SspI	AATATT	1 cut(s)	784
SspMI	CTAG	2 cut(s)	21, 867
StyD4I	CCNGG	5 cut(s)	115, 166, 920, 1086, 1281
StyI	CCWWGG	1 cut(s)	549
TaiI	ACGT	1 cut(s)	703
TaqI	TCGA	3 cut(s)	858, 1442, 1858
TasI	AATT	7 cut(s)	324, 580, 844, 1320, 1370, 1532, 1679
TauI	GCSGC	1 cut(s)	1642
TfiI	GAWTC	2 cut(s)	391, 1382
Tru1I	TTAA	5 cut(s)	311, 333, 1362, 1544, 1704
Tru9I	TTAA	5 cut(s)	311, 333, 1362, 1544, 1704
TscAI	CASTG	5 cut(s)	186, 409, 1195, 1237, 1354
TseFI	GTSAC	3 cut(s)	710, 1111, 1824
TseI	GCWGC	7 cut(s)	15, 206, 256, 263, 542, 545, 689
Tsp45I	GTSAC	3 cut(s)	710, 1111, 1824
TspDTI	ATGAA	6 cut(s)	470, 749, 753, 921, 1451, 1833
TspGWI	ACGGA	3 cut(s)	20, 535, 1429
TspRI	CASTG	5 cut(s)	186, 409, 1195, 1237, 1354
Van91I	CCANNNNNTGG	1 cut(s)	1217
VneI	GTGCAC	1 cut(s)	400
VpaK11BI	GGWCC	4 cut(s)	704, 1448, 1510, 1615
VspI	ATTAAT	1 cut(s)	333
XapI	RAATTY	3 cut(s)	580, 1370, 1679
XceI	RCATGY	1 cut(s)	648
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1048
XmiI	GTMKAC	1 cut(s)	697
XspI	CTAG	2 cut(s)	21, 867
Zsp2I	ATGCAT	1 cut(s)	40

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.