Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0005118

Physical Location & Seq

Genomic Coordinates Forward (+)

64992993 .. 64996320

Gene Structure 3328 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0518700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1698 bp

ATGCAGAGCGCCACCTCCCCCCTCCAAACCCTCCTCGACCTCATCAACGAGACCCTCTCCCTCATCCTCCGCACCTCCCTCACCGTCCGATCCTTCGCCGGCCGCTGGCAGCCCCTCCGCTCCAAGCTCGCCTCCCTCCAGACCTCCGTCGCCGACATCTCCGACTCCCCCCACTGGGCCGAGAACCCCCTCCTCTACACTCTCCTCCCCAACCTCCTCTCCACTCTCCAGCGCCTCAAGGCCCTCGCCGACCAATGCAACGACACGTCGTTCACCGGCGGGAAGCTCCACATGCAGAGCGACCTCGACATGGCCGCCGCGTCCCTCACCAACCAGCTCCGCGACCTCGACCTGCTGCTCAGATCTGGCGTGCTCCACCAGTCAAACGCCATCGTTTTGTCCCACCCGGGTCCCGGGTCGGATAAGGAGAATCTCAGCTTCTTTATCCGGGACCTCTTCACGCGTCTGCAAATCGGCGGCGTCGAGTTCAAGAAGAAGGCTCTGGAGTCTCTGCTTCAGCTTCTCGAAGACGACGACAAGGCCGCGACCCTCGTCGCCAAAGAAGGAAACGTCGCCTATTTGATTCACCTTCTCGATTTCCACAACCAGCCCTGGGTCCGAGAACAGGCCACCTCCGCCGTTTCGCTCCTCGCCGCCGCAAACGACGAGTCGCGCAAGGCGGTGTTCGAAGAAGGGGGTTTAGGCCCGTTGCTGAGACTGCTCGAATCCGGGACTAACACATTGAAAGAGAAGGCGGCAATCGCCGTCGAGGCACTCACCGTCGACCCGGAAAACGCCTGGGCCGTTTCAGCCTACGGCGGCGTTGCTGTATTGATCGAATCCATTCGATCCGGGACTCCGGCGACGCAGACCCACTCGGTCGGCGCCATCAGAAACGTGGCTAACGTGGAGGAGATCAAGACGGCTTTAGGAGAAGAAGGGGCTGTGCCAGTGCTACTCCAATTGCTCCTCTCCGGCACCATCACCGCCCAAGAGAAATCCGCCAATTGCATAGCGATTTTAGCCTCAACCGATGAGTATTTCCGAGCTTTGATCATCCAAGAACGCGGCCTGCAGAGATTGATGCATTTGATCCAGGACCTACTGAGCTCGGAGACATTGGAACACGTCCTCCGAGCCATCACTTCGCTTTCGGCATCAGAGCCCTGCGCTCGAGTCCTGTCATCTTCAACCTTGTTCCTAATTCAATTGGCCGAGTTCATAAAGCAGGGTAGCTCAATCCTCCAGCAAATCTCAGTTTCTTTGCTTTCGAATCTATCAATTAGTATCAGCGATGGGAATAAGCGAGCCATTAGTAGCTGTATGGGGTCATTGGTCAAGCTCATGGAGTCGCCAAAACCGGTGGGGCTACAAGAATCGGCGTCGCAAGCTCTTGTTTCGCTGTTGACGGTCCGGTCGAACCGGAAAGAGTTGGTTAGGGACGAGAAGAGCGTGATGAGGCTGATTCAGATGTTGGATCCCAGAAACGAAGCAGTGAGCTACAAGTTTCCGGTGATGGTAATCTCGGGGATACTCGCCGGAGGAAGCCAGGGATGCAGGAAGAGGCTCGCTGCGGCCGGAGCTCAGCCACATTTACAGAAGCTAGCGGAGATGGAGGTCGCCGGAGCCAAGAAGGCGTTGCAGAGACTCTCCGGCAATAGGCTAAAGACCATTTTCTCCCTGACTTGGAGGGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0097159 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

565

Amino Acids

61.23

Weight (kDa)

6.82

Isoelectric Point (pI)

41.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF7032	PF23005	16 - 124	1.8e-33	Domain of unknown function (DUF7032)
ARM_PUB	PF25598	164 - 346	2.1e-06	Plant U-box protein ARM repeat region
ARM_ARMC5	PF24768	178 - 382	1.9e-12	ARMC5

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005118)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G29390 AT3G29390
fragaria_vesca	FvH4_6g46910 FvH4_6g46910 FvH4_6g46910 FvH4_6g46910
malus_domestica	MD09G1066300.v1.1 MD09G1066400.v1.1
prunus_persica	Prupe.3G255200_v2.0.a1 Prupe.3G255200_v2.0.a1
pyrus_communis	pycom111g05570
rosa_chinensis	RchiOBHm_Chr2g0165881
rosa_laevigata	RLG00000021564
rosa_multiflora	Rmu_co8354311.1_g000001 Rmu_sc0001982.1_g000006 Rmu_sc0006218.1_g000001 Rmu_sc0006218.1_g000002 Rmu_sc0008709.1_g000009 Rmu_sc0013817.1_g000001
rosa_roxburghii	Rroxscaffold_2G00085390
rosa_rugosa	Rorug02G0518600 Rorug02G0518700 Rorug02G0518800 Rorug05G0064400 Rorug05G0462700
rosa_samantha	Rh2AG584500 Rh2BG596300 Rh2CG567200 Rh2DG606100
rosa_wichuraiana	Rw2G048730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1414
Acc36I	ACCTGC	1 cut(s)	360
AccB1I	GGYRCC	2 cut(s)	884, 977
AccBSI	CCGCTC	1 cut(s)	120
AccI	GTMKAC	1 cut(s)	783
AccII	CGCG	6 cut(s)	320, 342, 463, 545, 673, 1068
AclWI	GGATC	5 cut(s)	84, 843, 1087, 1472, 1485
AcoI	YGGCCR	4 cut(s)	100, 312, 1212, 1575
AcuI	CTGAAG	1 cut(s)	500
AcyI	GRCGYC	3 cut(s)	480, 885, 1382
AfiI	CCNNNNNNNGG	9 cut(s)	105, 174, 175, 310, 413, 613, 625, 1659, 1686
AflIII	ACRYGT	3 cut(s)	264, 461, 1126
AgeI	ACCGGT	1 cut(s)	1360
AgsI	TTSAA	4 cut(s)	490, 745, 1191, 1208
AjiI	CACGTC	2 cut(s)	267, 1129
AjnI	CCWGG	4 cut(s)	611, 797, 1095, 1548
AloI	GAACNNNNNNTCC	2 cut(s)	1116, 1148
Alw21I	GWGCWC	3 cut(s)	375, 1112, 1585
Alw26I	GTCTC	5 cut(s)	44, 513, 709, 1109, 1639
AlwI	GGATC	5 cut(s)	84, 843, 1087, 1472, 1485
Ama87I	CYCGRG	4 cut(s)	406, 413, 1173, 1525
ApeKI	GCWGC	3 cut(s)	109, 355, 1571
ArsI	GACNNNNNNTTYG	2 cut(s)	653, 685
AsiGI	ACCGGT	1 cut(s)	1360
Asp700I	GAANNNNTTC	1 cut(s)	843
AspLEI	GCGC	5 cut(s)	11, 234, 675, 887, 1172
AspS9I	GGNCC	9 cut(s)	177, 241, 410, 451, 616, 704, 801, 1099, 1411
AsuC2I	CCSGG	8 cut(s)	407, 408, 414, 415, 449, 730, 788, 853
AsuHPI	GGTGA	7 cut(s)	73, 265, 319, 578, 769, 976, 1525
AsuII	TTCGAA	2 cut(s)	687, 1271
AsuNHI	GCTAGC	1 cut(s)	1603
AvaI	CYCGRG	4 cut(s)	406, 413, 1173, 1525
AvaII	GGWCC	5 cut(s)	410, 451, 616, 1099, 1411
BamHI	GGATCC	1 cut(s)	1477
BanI	GGYRCC	2 cut(s)	884, 977
BanII	GRGCYC	3 cut(s)	1112, 1167, 1585
BbsI	GAAGAC	1 cut(s)	534
Bbv12I	GWGCWC	3 cut(s)	375, 1112, 1585
BbvI	GCAGC	3 cut(s)	121, 342, 1558
BccI	CCATC	7 cut(s)	398, 896, 989, 1148, 1289, 1510, 1606
BceAI	ACGGC	5 cut(s)	623, 749, 788, 832, 939
BciT130I	CCWGG	4 cut(s)	613, 799, 1097, 1550
BciVI	GTATCC	1 cut(s)	1524
BclI	TGATCA	1 cut(s)	1053
BcnI	CCSGG	8 cut(s)	407, 408, 414, 415, 449, 730, 788, 853
BcoDI	GTCTC	5 cut(s)	44, 513, 709, 1109, 1639
BfaI	CTAG	1 cut(s)	1604
BfmI	CTRYAG	1 cut(s)	1073
BfoI	RGCGCY	3 cut(s)	12, 235, 888
BfuAI	ACCTGC	1 cut(s)	360
BfuI	GTATCC	1 cut(s)	1524
BglI	GCCNNNNNGGC	2 cut(s)	770, 1634
BglII	AGATCT	1 cut(s)	362
BlpI	GCTNAGC	1 cut(s)	1584
Bme18I	GGWCC	5 cut(s)	410, 451, 616, 1099, 1411
BmeT110I	CYCGRG	4 cut(s)	406, 413, 1173, 1525
BmgBI	CACGTC	2 cut(s)	267, 1129
BmgT120I	GGNCC	9 cut(s)	177, 241, 410, 451, 616, 704, 801, 1099, 1411
BmiI	GGNNCC	8 cut(s)	411, 412, 452, 617, 886, 979, 1479, 1627
BmrI	ACTGGG	1 cut(s)	184
BmsI	GCATC	3 cut(s)	1074, 1166, 1544
BmtI	GCTAGC	1 cut(s)	1607
BmuI	ACTGGG	1 cut(s)	184
BoxI	GACNNNNGTC	2 cut(s)	146, 551
BpiI	GAAGAC	1 cut(s)	534
BplI	GAGNNNNNCTC	2 cut(s)	41, 73
BpmI	CTGGAG	4 cut(s)	122, 212, 524, 1229
Bpu1102I	GCTNAGC	1 cut(s)	1584
Bpu14I	TTCGAA	2 cut(s)	687, 1271
BpuEI	CTTGAG	1 cut(s)	221
BpuMI	CCSGG	8 cut(s)	407, 408, 414, 415, 449, 730, 788, 853
BsaBI	GATNNNNATC	1 cut(s)	1520
BsaHI	GRCGYC	3 cut(s)	480, 885, 1382
BsaI	GGTCTC	1 cut(s)	44
BsaJI	CCNNGG	6 cut(s)	406, 413, 611, 612, 798, 1549
BsaWI	WCCGGW	4 cut(s)	1360, 1413, 1422, 1510
BsaXI	ACNNNNNCTCC	4 cut(s)	203, 233, 1116, 1146
Bsc4I	CCNNNNNNNGG	9 cut(s)	105, 174, 175, 310, 413, 613, 625, 1659, 1686
Bse118I	RCCGGY	3 cut(s)	98, 275, 1360
Bse1I	ACTGG	3 cut(s)	179, 379, 950
Bse8I	GATNNNNATC	1 cut(s)	1520
BseBI	CCWGG	4 cut(s)	613, 799, 1097, 1550
BseDI	CCNNGG	6 cut(s)	406, 413, 611, 612, 798, 1549
BseGI	GGATG	3 cut(s)	63, 1056, 1559
BseJI	GATNNNNATC	1 cut(s)	1520
BseLI	CCNNNNNNNGG	9 cut(s)	105, 174, 175, 310, 413, 613, 625, 1659, 1686
BseMII	CTCAG	6 cut(s)	373, 448, 704, 1097, 1269, 1598
BseNI	ACTGG	3 cut(s)	179, 379, 950
BseRI	GAGGAG	7 cut(s)	23, 182, 194, 206, 638, 926, 959
BseX3I	CGGCCG	2 cut(s)	100, 1575
BseXI	GCAGC	3 cut(s)	121, 342, 1558
Bsh1236I	CGCG	6 cut(s)	320, 342, 463, 545, 673, 1068
Bsh1285I	CGRYCG	4 cut(s)	103, 882, 1418, 1578
BshNI	GGYRCC	2 cut(s)	884, 977
BshTI	ACCGGT	1 cut(s)	1360
BsiEI	CGRYCG	4 cut(s)	103, 882, 1418, 1578
BsiHKAI	GWGCWC	3 cut(s)	375, 1112, 1585
BsiHKCI	CYCGRG	4 cut(s)	406, 413, 1173, 1525
BslFI	GGGAC	7 cut(s)	307, 385, 396, 464, 745, 868, 1454
BslI	CCNNNNNNNGG	9 cut(s)	105, 174, 175, 310, 413, 613, 625, 1659, 1686
BsmAI	GTCTC	5 cut(s)	44, 513, 709, 1109, 1639
BsmFI	GGGAC	7 cut(s)	307, 385, 396, 464, 745, 868, 1454
Bso31I	GGTCTC	1 cut(s)	44
BsoBI	CYCGRG	4 cut(s)	406, 413, 1173, 1525
Bsp119I	TTCGAA	2 cut(s)	687, 1271
Bsp1286I	GDGCHC	4 cut(s)	375, 1112, 1167, 1585
Bsp143I	GATC	8 cut(s)	89, 362, 834, 848, 915, 1053, 1092, 1477
Bsp1720I	GCTNAGC	1 cut(s)	1584
BspCNI	CTCAG	6 cut(s)	372, 447, 705, 1098, 1268, 1597
BspFNI	CGCG	6 cut(s)	320, 342, 463, 545, 673, 1068
BspLI	GGNNCC	8 cut(s)	411, 412, 452, 617, 886, 979, 1479, 1627
BspMAI	CTGCAG	1 cut(s)	1077
BspMI	ACCTGC	1 cut(s)	360
BspOI	GCTAGC	1 cut(s)	1607
BspPI	GGATC	5 cut(s)	84, 843, 1087, 1472, 1485
BspQI	GCTCTTC	1 cut(s)	1442
BspT104I	TTCGAA	2 cut(s)	687, 1271
BspT107I	GGYRCC	2 cut(s)	884, 977
BspTNI	GGTCTC	1 cut(s)	44
BsrBI	CCGCTC	1 cut(s)	120
BsrFI	RCCGGY	3 cut(s)	98, 275, 1360
BsrI	ACTGG	3 cut(s)	179, 379, 950
BssAI	RCCGGY	3 cut(s)	98, 275, 1360
BssECI	CCNNGG	6 cut(s)	406, 413, 611, 612, 798, 1549
BssMI	GATC	8 cut(s)	89, 362, 834, 848, 915, 1053, 1092, 1477
BssNI	GRCGYC	3 cut(s)	480, 885, 1382
Bst2UI	CCWGG	4 cut(s)	613, 799, 1097, 1550
Bst4CI	ACNGT	3 cut(s)	85, 781, 1411
Bst6I	CTCTTC	3 cut(s)	461, 1442, 1556
BstACI	GRCGYC	3 cut(s)	480, 885, 1382
BstBI	TTCGAA	2 cut(s)	687, 1271
BstC8I	GCNNGC	9 cut(s)	100, 107, 129, 371, 1073, 1308, 1389, 1569, 1605
BstDEI	CTNAG	6 cut(s)	359, 434, 713, 1106, 1255, 1584
BstF5I	GGATG	3 cut(s)	63, 1056, 1559
BstFNI	CGCG	6 cut(s)	320, 342, 463, 545, 673, 1068
BstH2I	RGCGCY	3 cut(s)	12, 235, 888
BstHHI	GCGC	5 cut(s)	11, 234, 675, 887, 1172
BstKTI	GATC	8 cut(s)	92, 365, 837, 851, 918, 1056, 1095, 1480
BstMAI	GTCTC	5 cut(s)	44, 513, 709, 1109, 1639
BstMBI	GATC	8 cut(s)	89, 362, 834, 848, 915, 1053, 1092, 1477
BstMCI	CGRYCG	4 cut(s)	103, 882, 1418, 1578
BstNI	CCWGG	4 cut(s)	613, 799, 1097, 1550
BstNSI	RCATGY	1 cut(s)	295
BstPAI	GACNNNNGTC	2 cut(s)	146, 551
BstSFI	CTRYAG	1 cut(s)	1073
BstUI	CGCG	6 cut(s)	320, 342, 463, 545, 673, 1068
BstV1I	GCAGC	3 cut(s)	121, 342, 1558
BstV2I	GAAGAC	1 cut(s)	534
BstX2I	RGATCY	2 cut(s)	362, 1477
BstYI	RGATCY	2 cut(s)	362, 1477
BstZI	CGGCCG	2 cut(s)	100, 1575
BsuI	GTATCC	1 cut(s)	1524
BtgZI	GCGATG	1 cut(s)	1308
BtrI	CACGTC	2 cut(s)	267, 1129
BtsCI	GGATG	3 cut(s)	63, 1056, 1559
BtsI	GCAGTG	1 cut(s)	1500
BtsIMutI	CAGTG	3 cut(s)	172, 957, 1500
BveI	ACCTGC	1 cut(s)	360
Cac8I	GCNNGC	9 cut(s)	100, 107, 129, 371, 1073, 1308, 1389, 1569, 1605
CfoI	GCGC	5 cut(s)	11, 234, 675, 887, 1172
Cfr10I	RCCGGY	3 cut(s)	98, 275, 1360
Cfr13I	GGNCC	9 cut(s)	177, 241, 410, 451, 616, 704, 801, 1099, 1411
Cfr9I	CCCGGG	2 cut(s)	406, 413
CpoI	CGGWCCG	1 cut(s)	1411
CseI	GACGC	5 cut(s)	309, 452, 469, 874, 1371
CspAI	ACCGGT	1 cut(s)	1360
CspCI	CAANNNNNGTGG	2 cut(s)	1344, 1379
CspI	CGGWCCG	1 cut(s)	1411
CviAII	CATG	3 cut(s)	292, 310, 1345
DdeI	CTNAG	6 cut(s)	359, 434, 713, 1106, 1255, 1584
DinI	GGCGCC	1 cut(s)	886
DpnI	GATC	8 cut(s)	91, 364, 836, 850, 917, 1055, 1094, 1479
DpnII	GATC	8 cut(s)	89, 362, 834, 848, 915, 1053, 1092, 1477
DrdI	GACNNNNNNGTC	1 cut(s)	1414
DseDI	GACNNNNNNGTC	1 cut(s)	1414
EaeI	YGGCCR	4 cut(s)	100, 312, 1212, 1575
EagI	CGGCCG	2 cut(s)	100, 1575
Eam1104I	CTCTTC	3 cut(s)	461, 1442, 1556
EarI	CTCTTC	3 cut(s)	461, 1442, 1556
EciI	GGCGGA	2 cut(s)	625, 991
Ecl136II	GAGCTC	2 cut(s)	1110, 1583
EclXI	CGGCCG	2 cut(s)	100, 1575
Eco24I	GRGCYC	3 cut(s)	1112, 1167, 1585
Eco31I	GGTCTC	1 cut(s)	44
Eco47I	GGWCC	5 cut(s)	410, 451, 616, 1099, 1411
Eco52I	CGGCCG	2 cut(s)	100, 1575
Eco53kI	GAGCTC	2 cut(s)	1110, 1583
Eco57I	CTGAAG	1 cut(s)	500
Eco88I	CYCGRG	4 cut(s)	406, 413, 1173, 1525
EcoICRI	GAGCTC	2 cut(s)	1110, 1583
EcoO109I	RGGNCCY	4 cut(s)	241, 410, 451, 1099
EcoRII	CCWGG	4 cut(s)	611, 797, 1095, 1548
EcoT22I	ATGCAT	1 cut(s)	1089
EcoT38I	GRGCYC	3 cut(s)	1112, 1167, 1585
EgeI	GGCGCC	1 cut(s)	886
EheI	GGCGCC	1 cut(s)	886
FaeI	CATG	3 cut(s)	295, 313, 1348
FaiI	YATR	6 cut(s)	293, 311, 1013, 1223, 1325, 1346
FaqI	GGGAC	7 cut(s)	307, 385, 396, 464, 745, 868, 1454
FatI	CATG	3 cut(s)	291, 309, 1344
FauI	CCCGC	1 cut(s)	272
FbaI	TGATCA	1 cut(s)	1053
FblI	GTMKAC	1 cut(s)	783
FokI	GGATG	3 cut(s)	50, 1043, 1566
FriOI	GRGCYC	3 cut(s)	1112, 1167, 1585
FspBI	CTAG	1 cut(s)	1604
GlaI	GCGC	5 cut(s)	10, 233, 674, 886, 1171
GsuI	CTGGAG	4 cut(s)	122, 212, 524, 1229
HaeII	RGCGCY	3 cut(s)	12, 235, 888
HgaI	GACGC	5 cut(s)	309, 452, 469, 874, 1371
HhaI	GCGC	5 cut(s)	11, 234, 675, 887, 1172
Hin1I	GRCGYC	3 cut(s)	480, 885, 1382
Hin1II	CATG	3 cut(s)	295, 313, 1348
Hin6I	GCGC	5 cut(s)	9, 232, 673, 885, 1170
HinP1I	GCGC	5 cut(s)	9, 232, 673, 885, 1170
HincII	GTYRAC	2 cut(s)	784, 1407
HindII	GTYRAC	2 cut(s)	784, 1407
HphI	GGTGA	7 cut(s)	73, 265, 319, 578, 769, 976, 1525
Hpy166II	GTNNAC	3 cut(s)	273, 784, 1407
Hpy188III	TCNNGA	6 cut(s)	139, 490, 503, 524, 593, 919
Hpy8I	GTNNAC	3 cut(s)	273, 784, 1407
HpyAV	CCTTC	8 cut(s)	103, 490, 557, 599, 686, 745, 932, 1627
HpyCH4III	ACNGT	3 cut(s)	85, 781, 1411
HpyCH4IV	ACGT	5 cut(s)	266, 570, 897, 906, 1128
HpyCH4V	TGCA	9 cut(s)	4, 258, 295, 469, 1011, 1075, 1087, 1557, 1642
HpyF3I	CTNAG	6 cut(s)	359, 434, 713, 1106, 1255, 1584
HpySE526I	ACGT	5 cut(s)	266, 570, 897, 906, 1128
Hsp92I	GRCGYC	3 cut(s)	480, 885, 1382
Hsp92II	CATG	3 cut(s)	295, 313, 1348
HspAI	GCGC	5 cut(s)	9, 232, 673, 885, 1170
KasI	GGCGCC	1 cut(s)	884
KflI	GGGWCCC	1 cut(s)	410
KroI	GCCGGC	1 cut(s)	98
KroNI	GCCGGC	1 cut(s)	100
Ksp22I	TGATCA	1 cut(s)	1053
Kzo9I	GATC	8 cut(s)	89, 362, 834, 848, 915, 1053, 1092, 1477
LguI	GCTCTTC	1 cut(s)	1442
LmnI	GCTCC	8 cut(s)	125, 291, 342, 378, 651, 972, 1580, 1625
Lsp1109I	GCAGC	3 cut(s)	121, 342, 1558
LweI	GCATC	3 cut(s)	1074, 1166, 1544
MaeI	CTAG	1 cut(s)	1604
MaeII	ACGT	5 cut(s)	266, 570, 897, 906, 1128
MalI	GATC	8 cut(s)	91, 364, 836, 850, 917, 1055, 1094, 1479
MbiI	CCGCTC	1 cut(s)	120
MboI	GATC	8 cut(s)	89, 362, 834, 848, 915, 1053, 1092, 1477
MboII	GAAGA	8 cut(s)	448, 505, 539, 701, 947, 1179, 1459, 1573
MfeI	CAATTG	3 cut(s)	962, 1006, 1208
MflI	RGATCY	2 cut(s)	362, 1477
MhlI	GDGCHC	4 cut(s)	375, 1112, 1167, 1585
MluCI	AATT	5 cut(s)	962, 1006, 1203, 1208, 1281
MluI	ACGCGT	1 cut(s)	461
Mly113I	GGCGCC	1 cut(s)	885
MlyI	GAGTC	7 cut(s)	158, 515, 677, 850, 1185, 1358, 1641
MmeI	TCCRAC	3 cut(s)	186, 399, 1455
Mph1103I	ATGCAT	1 cut(s)	1089
MroNI	GCCGGC	1 cut(s)	98
MroXI	GAANNNNTTC	1 cut(s)	843
MspA1I	CMGCKG	1 cut(s)	105
MunI	CAATTG	3 cut(s)	962, 1006, 1208
MvaI	CCWGG	4 cut(s)	613, 799, 1097, 1550
MvnI	CGCG	6 cut(s)	320, 342, 463, 545, 673, 1068
NaeI	GCCGGC	1 cut(s)	100
NarI	GGCGCC	1 cut(s)	885
NciI	CCSGG	8 cut(s)	407, 408, 414, 415, 449, 730, 788, 853
NdeII	GATC	8 cut(s)	89, 362, 834, 848, 915, 1053, 1092, 1477
NgoMIV	GCCGGC	1 cut(s)	98
NheI	GCTAGC	1 cut(s)	1603
NlaIII	CATG	3 cut(s)	295, 313, 1348
NlaIV	GGNNCC	8 cut(s)	411, 412, 452, 617, 886, 979, 1479, 1627
NmeAIII	GCCGAG	2 cut(s)	205, 1240
NsiI	ATGCAT	1 cut(s)	1089
NspI	RCATGY	1 cut(s)	295
NspV	TTCGAA	2 cut(s)	687, 1271
PaeR7I	CTCGAG	1 cut(s)	1173
PasI	CCCWGGG	1 cut(s)	612
PciSI	GCTCTTC	1 cut(s)	1442
PcsI	WCGNNNNNNNCGW	6 cut(s)	480, 531, 801, 903, 1415, 1449
PdiI	GCCGGC	1 cut(s)	100
PdmI	GAANNNNTTC	1 cut(s)	843
PfeI	GAWTC	7 cut(s)	430, 583, 725, 839, 1273, 1376, 1465
PfoI	TCCNGGA	4 cut(s)	447, 728, 851, 1095
PinAI	ACCGGT	1 cut(s)	1360
PleI	GAGTC	7 cut(s)	158, 514, 676, 850, 1184, 1357, 1641
PluTI	GGCGCC	1 cut(s)	888
PpsI	GAGTC	7 cut(s)	158, 514, 676, 850, 1184, 1357, 1641
PpuMI	RGGWCCY	3 cut(s)	410, 451, 1099
PshAI	GACNNNNGTC	2 cut(s)	146, 551
Psp124BI	GAGCTC	2 cut(s)	1112, 1585
Psp5II	RGGWCCY	3 cut(s)	410, 451, 1099
Psp6I	CCWGG	4 cut(s)	611, 797, 1095, 1548
PspGI	CCWGG	4 cut(s)	611, 797, 1095, 1548
PspN4I	GGNNCC	8 cut(s)	411, 412, 452, 617, 886, 979, 1479, 1627
PspPI	GGNCC	9 cut(s)	177, 241, 410, 451, 616, 704, 801, 1099, 1411
PspPPI	RGGWCCY	3 cut(s)	410, 451, 1099
PspXI	VCTCGAGB	1 cut(s)	1173
PstI	CTGCAG	1 cut(s)	1077
PsuI	RGATCY	2 cut(s)	362, 1477
Rsr2I	CGGWCCG	1 cut(s)	1411
RsrII	CGGWCCG	1 cut(s)	1411
SacI	GAGCTC	2 cut(s)	1112, 1585
SalI	GTCGAC	1 cut(s)	782
SapI	GCTCTTC	1 cut(s)	1442
Sau3AI	GATC	8 cut(s)	89, 362, 834, 848, 915, 1053, 1092, 1477
Sau96I	GGNCC	9 cut(s)	177, 241, 410, 451, 616, 704, 801, 1099, 1411
SchI	GAGTC	7 cut(s)	158, 515, 677, 850, 1185, 1358, 1641
SduI	GDGCHC	4 cut(s)	375, 1112, 1167, 1585
SfaNI	GCATC	3 cut(s)	1074, 1166, 1544
SfcI	CTRYAG	1 cut(s)	1073
SfoI	GGCGCC	1 cut(s)	886
Sfr274I	CTCGAG	1 cut(s)	1173
SfuI	TTCGAA	2 cut(s)	687, 1271
SgrAI	CRCCGGYG	1 cut(s)	275
SinI	GGWCC	5 cut(s)	410, 451, 616, 1099, 1411
SlaI	CTCGAG	1 cut(s)	1173
SmaI	CCCGGG	2 cut(s)	408, 415
SmlI	CTYRAG	2 cut(s)	236, 1173
SmoI	CTYRAG	2 cut(s)	236, 1173
Sse9I	AATT	5 cut(s)	962, 1006, 1203, 1208, 1281
SspDI	GGCGCC	1 cut(s)	884
SspMI	CTAG	1 cut(s)	1604
SstI	GAGCTC	2 cut(s)	1112, 1585
TaaI	ACNGT	3 cut(s)	85, 781, 1411
TaiI	ACGT	5 cut(s)	269, 573, 900, 909, 1131
TaqII	GACCGA	1 cut(s)	868
TasI	AATT	5 cut(s)	962, 1006, 1203, 1208, 1281
TfiI	GAWTC	7 cut(s)	430, 583, 725, 839, 1273, 1376, 1465
TscAI	CASTG	3 cut(s)	179, 957, 1500
TseI	GCWGC	3 cut(s)	109, 355, 1571
TspDTI	ATGAA	1 cut(s)	1210
TspGWI	ACGGA	1 cut(s)	136
TspMI	CCCGGG	2 cut(s)	406, 413
TspRI	CASTG	3 cut(s)	179, 957, 1500
VpaK11BI	GGWCC	5 cut(s)	410, 451, 616, 1099, 1411
XceI	RCATGY	1 cut(s)	295
XcmI	CCANNNNNNNNNTGG	1 cut(s)	895
XhoI	CTCGAG	1 cut(s)	1173
XmaI	CCCGGG	2 cut(s)	406, 413
XmiI	GTMKAC	1 cut(s)	783
XmnI	GAANNNNTTC	1 cut(s)	843
XspI	CTAG	1 cut(s)	1604
Zsp2I	ATGCAT	1 cut(s)	1089

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.