MD09G1066400.v1.1

Protein RIK isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005118
Physical Location & Seq
Forward (+)
4519348 .. 4523788
4441 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1066400.v1.1.491

Sequence Viewer

Length: 1044 bp
ATGAATGAAACAGGAGCAACTGTCTCACTCAGAGGGCGTGGTTCCGGAAATATTGAGAATATACATGACGAAGAAGGACAGCTACCGTTGCATTTATTCTTGTCAAGTAATAATTCAAAAAGTCTTGAAGATGCTAAGCTTTTGGCTGAAAATCTTTTAGATACAATCAGTGTAGAGTGTGGTGTCTCCAGGGTTTCATCATCTAAGGTTTATAGTGCTGTTGCACCCCCACAGCAGGTATATAGTGCTGTTCCACCACCGCATCAGTTGGCCGGGGTTCAGAGTTCTGGAATTGAGGTAAAAGCAATTACAAGTTCGGTGTCTCCAACTGTGGGTGCTACACCAGCTTTGCCAGTTTCCTCTGCTGGAACCCCTGGTGTCGCTACTGTCTTTTCTCAAGGGACAGTCTCTCAACCTGGAGGATTGTTGAACTGTGCACAATCTCAGGCGAACATTGGTGGTTATCCCCAACCTCTATTATCTAGTGGAACAAGCTACAATGGATACGCAGGGATATATCCTCAAGTCACACCTTTGCAACAGGTTGCTCTGGCCCTTAGACAGCCATCTTCCCCTATCACTTCTACAGTTGCTCCCACAACATCAGCCCCAAGCACTGAACCAAAGGTGAATGTTACCTCTGGTTCTGAGAAGGAGAAACACCCTCCACAAAGGCGGAAGTTTCAGGAGTTACCAGTTGGTCCTGCAAAAGTCCATCAGGATTTGGGTGTGAGAAATGTATCCACGATGCCAGCTCCAAAGAAACTGGTCCAGCAGGCATCAAATGGAATGCCACCTCCCCCACCTAGAGCCATGCCTCCAGCTCCACCTCCACCAAAATTTACCTCATCAACACAGGCTGTTAAAGTGCTTGACAAGAACATTGCTCTGAAGAAAACAAAACCTGATAATGTTCCTGATACTCTGGTCAAGCTAATGGAATATGGTGATGATGAAGATGATGACGATGAGGAAACTAATGAAGAATTACCTAATAACAACTCCGGTCCAGTGACAGCTCGAAAGCCTTTCTGGGCCTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

348

Amino Acids

36.4

Weight (kDa)

6.0

Isoelectric Point (pI)

60.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KH-I_KHDC4-BBP PF22675 1 - 54 2.8e-16 KHDC4/BBP-like, KH-domain type I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 226
AccIII TCCGGA 1 cut(s) 44
AciI CCGC 2 cut(s) 260, 676
AcoI YGGCCR 1 cut(s) 270
AcsI RAATTY 1 cut(s) 839
AcuI CTGAAG 1 cut(s) 911
AfiI CCNNNNNNNGG 2 cut(s) 235, 332
AgsI TTSAA 3 cut(s) 117, 128, 430
AjnI CCWGG 3 cut(s) 188, 373, 415
AluBI AGCT 8 cut(s) 82, 139, 347, 495, 755, 824, 934, 1019
AluI AGCT 8 cut(s) 82, 139, 347, 495, 755, 824, 934, 1019
Alw21I GWGCWC 1 cut(s) 439
Alw26I GTCTC 4 cut(s) 28, 190, 327, 412
Alw44I GTGCAC 1 cut(s) 435
Aor13HI TCCGGA 1 cut(s) 44
AoxI GGCC 3 cut(s) 270, 552, 1035
ApaLI GTGCAC 1 cut(s) 435
ApoI RAATTY 1 cut(s) 839
AspS9I GGNCC 5 cut(s) 553, 701, 769, 1007, 1035
AsuC2I CCSGG 1 cut(s) 274
AsuHPI GGTGA 2 cut(s) 640, 959
AvaII GGWCC 3 cut(s) 701, 769, 1007
BaeGI GKGCMC 1 cut(s) 439
BaeI ACNNNNGTAYC 2 cut(s) 912, 945
BarI GAAGNNNNNNTAC 2 cut(s) 66, 98
Bbv12I GWGCWC 1 cut(s) 439
BccI CCATC 2 cut(s) 574, 723
BciT130I CCWGG 3 cut(s) 190, 375, 417
BciVI GTATCC 2 cut(s) 497, 751
BcnI CCSGG 1 cut(s) 274
BcoDI GTCTC 4 cut(s) 28, 190, 327, 412
BfaI CTAG 2 cut(s) 483, 807
BfmI CTRYAG 1 cut(s) 585
BfuAI ACCTGC 1 cut(s) 226
BfuI GTATCC 2 cut(s) 497, 751
BlpI GCTNAGC 1 cut(s) 135
Bme1390I CCNGG 4 cut(s) 190, 274, 375, 417
Bme18I GGWCC 3 cut(s) 701, 769, 1007
BmgT120I GGNCC 5 cut(s) 553, 701, 769, 1007, 1035
BmiI GGNNCC 2 cut(s) 43, 370
BmrFI CCNGG 4 cut(s) 190, 274, 375, 417
BmsI GCATC 4 cut(s) 121, 271, 738, 788
BpmI CTGGAG 3 cut(s) 172, 438, 804
Bpu1102I GCTNAGC 1 cut(s) 135
BpuEI CTTGAG 2 cut(s) 381, 507
BpuMI CCSGG 1 cut(s) 274
BsaJI CCNNGG 3 cut(s) 189, 273, 373
BsaWI WCCGGW 2 cut(s) 44, 1004
BsaXI ACNNNNNCTCC 4 cut(s) 6, 36, 577, 607
Bsc4I CCNNNNNNNGG 2 cut(s) 235, 332
Bse1I ACTGG 4 cut(s) 353, 695, 771, 1010
Bse3DI GCAATG 1 cut(s) 882
BseAI TCCGGA 1 cut(s) 44
BseBI CCWGG 3 cut(s) 190, 375, 417
BseDI CCNNGG 3 cut(s) 189, 273, 373
BseLI CCNNNNNNNGG 2 cut(s) 235, 332
BseMI GCAATG 1 cut(s) 882
BseMII CTCAG 3 cut(s) 43, 458, 639
BseNI ACTGG 4 cut(s) 353, 695, 771, 1010
BseSI GKGCMC 1 cut(s) 439
BshFI GGCC 3 cut(s) 272, 554, 1037
BsiHKAI GWGCWC 1 cut(s) 439
BsiSI CCGG 3 cut(s) 45, 273, 1005
BslFI GGGAC 1 cut(s) 415
BslI CCNNNNNNNGG 2 cut(s) 235, 332
BsmAI GTCTC 4 cut(s) 28, 190, 327, 412
BsmFI GGGAC 1 cut(s) 415
BsmI GAATGC 1 cut(s) 795
BsnI GGCC 3 cut(s) 272, 554, 1037
Bsp1286I GDGCHC 1 cut(s) 439
Bsp13I TCCGGA 1 cut(s) 44
Bsp1720I GCTNAGC 1 cut(s) 135
BspACI CCGC 2 cut(s) 260, 676
BspANI GGCC 3 cut(s) 272, 554, 1037
BspCNI CTCAG 3 cut(s) 42, 457, 640
BspEI TCCGGA 1 cut(s) 44
BspLI GGNNCC 2 cut(s) 43, 370
BspMI ACCTGC 1 cut(s) 226
BsrDI GCAATG 1 cut(s) 882
BsrI ACTGG 4 cut(s) 353, 695, 771, 1010
BssECI CCNNGG 3 cut(s) 189, 273, 373
Bst2UI CCWGG 3 cut(s) 190, 375, 417
Bst4CI ACNGT 7 cut(s) 22, 87, 331, 388, 406, 434, 589
BstC8I GCNNGC 2 cut(s) 753, 777
BstDEI CTNAG 6 cut(s) 29, 135, 204, 444, 557, 648
BstMAI GTCTC 4 cut(s) 28, 190, 327, 412
BstMWI GCNNNNNNNGC 2 cut(s) 88, 344
BstNI CCWGG 3 cut(s) 190, 375, 417
BstSCI CCNGG 4 cut(s) 188, 272, 373, 415
BstSFI CTRYAG 1 cut(s) 585
BstSLI GKGCMC 1 cut(s) 439
BsuI GTATCC 2 cut(s) 497, 751
BsuRI GGCC 3 cut(s) 272, 554, 1037
BtsIMutI CAGTG 3 cut(s) 175, 615, 1017
BveI ACCTGC 1 cut(s) 226
Cac8I GCNNGC 2 cut(s) 753, 777
Cfr13I GGNCC 5 cut(s) 553, 701, 769, 1007, 1035
CviAII CATG 2 cut(s) 65, 814
DdeI CTNAG 6 cut(s) 29, 135, 204, 444, 557, 648
EaeI YGGCCR 1 cut(s) 270
EciI GGCGGA 1 cut(s) 691
Eco47I GGWCC 3 cut(s) 701, 769, 1007
Eco57I CTGAAG 1 cut(s) 911
EcoRII CCWGG 3 cut(s) 188, 373, 415
FaeI CATG 2 cut(s) 68, 817
FaiI YATR 9 cut(s) 62, 66, 213, 241, 243, 517, 815, 945, 1042
FaqI GGGAC 1 cut(s) 415
FatI CATG 2 cut(s) 64, 813
FspBI CTAG 2 cut(s) 483, 807
GsuI CTGGAG 3 cut(s) 172, 438, 804
HaeIII GGCC 3 cut(s) 272, 554, 1037
HapII CCGG 3 cut(s) 45, 273, 1005
Hin1II CATG 2 cut(s) 68, 817
HindIII AAGCTT 1 cut(s) 137
HpaII CCGG 3 cut(s) 45, 273, 1005
HphI GGTGA 2 cut(s) 640, 959
Hpy166II GTNNAC 1 cut(s) 437
Hpy188I TCNGA 4 cut(s) 32, 282, 649, 891
Hpy188III TCNNGA 6 cut(s) 45, 125, 288, 686, 719, 917
Hpy8I GTNNAC 1 cut(s) 437
HpyAV CCTTC 2 cut(s) 68, 646
HpyCH4III ACNGT 7 cut(s) 22, 87, 331, 388, 406, 434, 589
HpyCH4V TGCA 5 cut(s) 91, 224, 437, 538, 707
HpyF10VI GCNNNNNNNGC 2 cut(s) 88, 344
HpyF3I CTNAG 6 cut(s) 29, 135, 204, 444, 557, 648
Hsp92II CATG 2 cut(s) 68, 817
Kpn2I TCCGGA 1 cut(s) 44
LmnI GCTCC 4 cut(s) 14, 598, 760, 829
LweI GCATC 4 cut(s) 121, 271, 738, 788
MaeI CTAG 2 cut(s) 483, 807
MaeIII GTNAC 4 cut(s) 526, 634, 690, 1012
MboII GAAGA 6 cut(s) 83, 140, 561, 904, 968, 995
MhlI GDGCHC 1 cut(s) 439
MluCI AATT 5 cut(s) 112, 291, 306, 839, 986
MmeI TCCRAC 1 cut(s) 350
MroI TCCGGA 1 cut(s) 44
MseI TTAA 1 cut(s) 864
MspI CCGG 3 cut(s) 45, 273, 1005
MspR9I CCNGG 4 cut(s) 190, 274, 375, 417
Mva1269I GAATGC 1 cut(s) 795
MvaI CCWGG 3 cut(s) 190, 375, 417
MwoI GCNNNNNNNGC 2 cut(s) 88, 344
NciI CCSGG 1 cut(s) 274
NlaIII CATG 2 cut(s) 68, 817
NlaIV GGNNCC 2 cut(s) 43, 370
NmuCI GTSAC 2 cut(s) 526, 1012
PctI GAATGC 1 cut(s) 795
Psp6I CCWGG 3 cut(s) 188, 373, 415
PspGI CCWGG 3 cut(s) 188, 373, 415
PspN4I GGNNCC 2 cut(s) 43, 370
PspPI GGNCC 5 cut(s) 553, 701, 769, 1007, 1035
SaqAI TTAA 1 cut(s) 864
Sau96I GGNCC 5 cut(s) 553, 701, 769, 1007, 1035
ScrFI CCNGG 4 cut(s) 190, 274, 375, 417
SduI GDGCHC 1 cut(s) 439
SfaNI GCATC 4 cut(s) 121, 271, 738, 788
SfcI CTRYAG 1 cut(s) 585
SinI GGWCC 3 cut(s) 701, 769, 1007
SmlI CTYRAG 2 cut(s) 396, 522
SmoI CTYRAG 2 cut(s) 396, 522
Sse9I AATT 5 cut(s) 112, 291, 306, 839, 986
SsiI CCGC 2 cut(s) 260, 676
SspI AATATT 1 cut(s) 52
SspMI CTAG 2 cut(s) 483, 807
StyD4I CCNGG 4 cut(s) 188, 272, 373, 415
TaaI ACNGT 7 cut(s) 22, 87, 331, 388, 406, 434, 589
TaqI TCGA 1 cut(s) 1021
TasI AATT 5 cut(s) 112, 291, 306, 839, 986
Tru1I TTAA 1 cut(s) 864
Tru9I TTAA 1 cut(s) 864
TscAI CASTG 3 cut(s) 175, 622, 1017
TseFI GTSAC 2 cut(s) 526, 1012
Tsp45I GTSAC 2 cut(s) 526, 1012
TspDTI ATGAA 5 cut(s) 17, 21, 186, 969, 996
TspRI CASTG 3 cut(s) 175, 622, 1017
VneI GTGCAC 1 cut(s) 435
VpaK11BI GGWCC 3 cut(s) 701, 769, 1007
XapI RAATTY 1 cut(s) 839
XspI CTAG 2 cut(s) 483, 807
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.