Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0003739

Physical Location & Seq

Genomic Coordinates Forward (+)

5411911 .. 5419100

Gene Structure 7190 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1076800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3078 bp

ATGTCCTGCGCCCAAACCCCCTTTTCCCCTGCAAATTTCACAAAGAATTTCACATTTCGAATCCCCATAGGTTTCCACAAGAACCCATCAATGATATCCACAAGTTTGTGGAGAAACTTGGCTAAGCAAGCCATTCCCAGGAACCAGAGGGGTCGTCTCAATTCACAGTCCATTTCTCGAGCTTATAGCCTTCTGTGTTTTTCCTTGGAATCTGTTTCCCCGCAATTGTTCAAGCCTTTTCAGCTGGGTTCTTACTCAAACATGGGTTTTCCGGAGGTGGGTTTTCCCGGGAAAGGTGAAATTTCTACAAGTGAGAGTTATTTGGGCAACCCAAGTTATTGGTCTGTGAGGACTCCAATTGGGTTTAATGGGTTTTGCGCCAAAGGGTATGCGAGCGTCGCTGAGGTTGTTTCTTCCACTGATGCGGAGGAGGATGTGTCTCTTGTAGATGAAGTTCAAGAACTGTTGCAGCAAATGAGTAAAGAGGGTAAGAGGCAGACTGACCATTGGCGGCGGATGCGGCTGAGGAGAGTGAGAGGAATGGGGCAGGCGAAGTACCTTGCACTGAAGAGAAGACAGATAAAGATTGAGACTGAAGCGTGGGAACGGGCAGCGAAGGAGTACAGGGAGCTTATGATGGACATGTGTGAGCACAAGTTAGCTCCCAATTTGCCATACATGAAGTCTTTGTTTCTTGGTTGGTTTGAGCCTATGCGCAATGCCATTGAAAAAGAGCAAGACCTGTGCCGGAAAGGGAAGAATAAGGCAGCCTACGCACCTTATATCGATCAGTTACCAGCTGATATGATGTCGGTTATTACAATGCATAAGCTGATGGGATTGTTGATGACCGGAGGCGAACATGGAACTTGCAGGGTGGTTGCAGCTGCTTGTACCATAGGTGAAGCCATTGAGCACGAGGTTAGAATACACAATTTCCTGGAACGGACAAGAAAGAAAAAGGTCAACAAAGATGAGGAAAACCGAGTGTCTGATGATGTATTGAAGGAACAAGAGAAGCTGAATAAAAAGGTCACTAATTTGATGAAAAAGCAAAAACTGCAAGTGGTGAGACATATAGTGCGGAAACATGATGCTTCAAAACCCTGGAGCCAGGATGCCAGGGCTAAGGTCGGCAGTCGTCTGATTGAATTGTTGATTCAAACGGCTTTTATACAACCTCCGGCCGATCAGTTAGCAGATGGTCCACCAGATATACGACCTGCATTTATACACACATTTAGGACTGTCATGAAAGATCCAAAGAATACGTCCAGCAGAAGATTTGGTGTTATTCAATGTGACCCTCTTGTTCTTAAAGGCTTTGAGAAAACAGGAAGCCACATTGTGATTCCTTATATGCCGATGTTGGTGCCACCAGTCAACTGGACAGGTTATGATAGAGGAGGGTACTTTTTCCTACCATCCTATGTCATGCGCACTCATGGAGCTAAACAGCAGCGTGAAGCAATAAAGAGGACCCCAAGGGAGCAACTGGAACCAGTTTTTAAGGCCCTGGATACTCTTGGAAATACCAGATGGAAGGTTAATAAGAGGGTACTTAATGTTGTAGATAGAATATGGGCTAGTGGAGGCCGTCTTGCTGACTTGGTGGACCGTGATAATGTTCCTTTACCAGAGGAACCAGATACTGAAGATGAAAAACTGCTTAAGAAATGGAAATGGAAGGTGAAATCTGTGAAGAAAGAGAACATGGAGAGGCATTCCCAGCGTTGTGACTTGGAGCTTAAATTAACTGTAGCACGGGAGATGAAGGATGCAGAAGGTTTTTACTATCCACACAATCTTGATTTTCGGGGCCGTGCATATCCTATGCACCCATACTTAAATCATCTTGGTTCAGATCTATCCCGGGGTATTTTGGAGTTCGCAGAGGGACGCCCCCTTGGAAAGTCAGGCTTACGTTGGCTGAAGATACACTTGGCAAATCTTTATGCGAGTGGTGTAGACAAATTATCTCGCGAGGGTCGAATAGCATTTATAGAGAATCATTTGGATGATATATTTGATTCTGTGGACAGACCGCTAGAAGGAAGACGCTGGTGGTTGAAGGCAGAAGATCCATTTCAGTGCTTGGCAGTGTGCATAAACCTAGCTGAAGCTTTGAGAAGCTCTTCTCCAGAGACTTTTATTTCACATATACCCGTACATCAGGATGGCTCTTGCAATGGTTTACAACACTACGCTGCCCTTGGAAGAGACAAGTTGGGAGCAGCTTCTGTCAATCTTGTTGCAGGAGAGAAGCCTGCAGATGTATACTCAGGAATAGCTGCTAGGGTACTGGATATCATGCGAAGGGATGCACAGAAGGATCCTGCTGTTTTTCCAGAGGCATTGCGTGCAAAAGTGTTGGTTGATCAGGTGGATAGGAAATTGGTGAAGCAGACAGTGATGACATCAGTATATGGTGTCACTTACATTGGTGCTCGGGATCAAATCAAGAGGAGGTTGAAGGAACGCGGTTCCATTTCAGATGATGAAGAGCTTTTTGGTTGTGCTTGTTATGCTGCAAAGATCACCTTAACCGCTCTAGGTGAGATGTTTGAAGCTGCACGAAGTATCATGAGCTGGCTTGGCGAATGTGCAAAAATAATTGCATCTGAAAATGAGCCAGTACGATGGACAACTCCGCTCGGACTTCCAGTTGTGCAACCTTACCGTAAATTCGGCAGGCATCTTATTAAAACTTCGCTTCAAGTTTTATCGTTGCAGCAAGAAACAGATAAGGTCATGGTCAAGAGGCAGAGGACAGCGTTCCCACCAAATTTTGTTCATTCCCTCGATAGTTCTCACATGATGATGACTGCGGTTGCCTGTAAAAAAGCAGGCTTAAACTTTGCAGGAGTTCATGACTCGTACTGGACGCATGCTTGTGATGTGGATGAAATGAACAGGATACTTCGGGAAAAATTTGTCGAACTCTATGAGATTCCAATACTAGAGAATTTGTTGGAGAGCTTTCAGCAGTCCTTTCCCACATTAGCTTTTCCTGCCTTGCCCGAACGAGGTGACTTTGACTTAAGAGACGTGTTAGAGTCACCTTATTTCTTCAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0000959 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006351 GO:0006390 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030880 GO:0032774 GO:0032991 GO:0034062 GO:0034245 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0061695 GO:0071704 GO:0090304 GO:0097659 GO:0097747 GO:0098798 GO:0140053 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RNA_pol RPOL_N

Protein Analysis

1026

Amino Acids

116.85

Weight (kDa)

9.22

Isoelectric Point (pI)

49.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPOL_N	PF14700	192 - 511	2.7e-86	DNA-directed RNA polymerase N-terminal
RNA_pol	PF00940	634 - 999	7.8e-161	DNA-dependent RNA polymerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003739)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G15700 AT5G15700
fragaria_vesca	FvH4_6g46120 FvH4_6g46120 FvH4_6g46120 FvH4_6g46120 FvH4_6g46120 FvH4_6g46120 FvH4_6g46120 FvH4_6g46120
malus_domestica	MD09G1076800.v1.1 MD15G1148800.v1.1 MD17G1067300.v1.1
prunus_persica	Prupe.3G247300_v2.0.a1 Prupe.3G247300_v2.0.a1 Prupe.3G247300_v2.0.a1 Prupe.3G247300_v2.0.a1
pyrus_communis	pycom09g00400 pycom17g06760
rosa_chinensis	RchiOBHm_Chr2g0164571
rosa_laevigata	RLG00000021466
rosa_multiflora	Rmu_co8270321.1_g000001 Rmu_sc0020353.1_g000005 Rmu_ssc0000076.1_g000002
rosa_roxburghii	Rroxscaffold_2G00086480
rosa_rugosa	Rorug02G0509000 Rorug02G0509100 Rorug02G0509100 Rorug02G0509200.1
rosa_samantha	Rh2AG576800 Rh2BG587900 Rh2CG558700 Rh2DG598500
rosa_wichuraiana	Rw2G047890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	2 cut(s)	716, 1439
Acc36I	ACCTGC	1 cut(s)	1231
AccB1I	GGYRCC	1 cut(s)	1372
AccBSI	CCGCTC	2 cut(s)	2549, 2653
AccI	GTMKAC	2 cut(s)	1968, 2277
AccII	CGCG	2 cut(s)	1983, 2481
AccIII	TCCGGA	1 cut(s)	271
AclWI	GGATC	5 cut(s)	1253, 2075, 2327, 2340, 2460
AcoI	YGGCCR	1 cut(s)	1185
AcsI	RAATTY	7 cut(s)	34, 46, 300, 2684, 2785, 2930, 2965
AcuI	CTGAAG	5 cut(s)	587, 615, 1674, 1952, 2139
AcyI	GRCGYC	1 cut(s)	1900
AdeI	CACNNNGTG	1 cut(s)	1348
AfaI	GTAC	9 cut(s)	557, 623, 895, 1412, 1560, 2169, 2301, 2637, 2879
AfiI	CCNNNNNNNGG	5 cut(s)	138, 278, 293, 2051, 3026
AflII	CTTAAG	2 cut(s)	1670, 3040
AflIII	ACRYGT	2 cut(s)	642, 3048
AjiI	CACGTC	1 cut(s)	3049
AjnI	CCWGG	6 cut(s)	137, 939, 1106, 1113, 1121, 1515
AjuI	GAANNNNNNNTTGG	4 cut(s)	1925, 1957, 2493, 2525
Alw21I	GWGCWC	3 cut(s)	654, 918, 2449
Alw26I	GTCTC	7 cut(s)	161, 444, 584, 1066, 2138, 2214, 3039
AlwI	GGATC	5 cut(s)	1253, 2075, 2327, 2340, 2460
AlwNI	CAGNNNCTG	2 cut(s)	1652, 2240
Ama87I	CYCGRG	4 cut(s)	177, 287, 1872, 2448
Aor13HI	TCCGGA	1 cut(s)	271
AoxI	GGCC	4 cut(s)	1185, 1512, 1594, 1819
ApoI	RAATTY	7 cut(s)	34, 46, 300, 2684, 2785, 2930, 2965
ArsI	GACNNNNNNTTYG	2 cut(s)	1256, 1288
Asp700I	GAANNNNTTC	1 cut(s)	2134
AspLEI	GCGC	4 cut(s)	11, 380, 717, 1440
AspS9I	GGNCC	5 cut(s)	1205, 1479, 1513, 1615, 1819
AsuC2I	CCSGG	4 cut(s)	288, 289, 1873, 1874
AsuHPI	GGTGA	9 cut(s)	308, 914, 1081, 1702, 2410, 2530, 2567, 3041, 3051
AsuII	TTCGAA	1 cut(s)	58
AvaI	CYCGRG	4 cut(s)	177, 287, 1872, 2448
AvaII	GGWCC	3 cut(s)	1205, 1479, 1615
BamHI	GGATCC	1 cut(s)	2332
BanI	GGYRCC	1 cut(s)	1372
BauI	CACGAG	1 cut(s)	917
BbsI	GAAGAC	2 cut(s)	580, 2062
Bbv12I	GWGCWC	3 cut(s)	654, 918, 2449
BbvCI	CCTCAGC	2 cut(s)	402, 524
BccI	CCATC	8 cut(s)	94, 631, 829, 1196, 1432, 1533, 2171, 2634
BceAI	ACGGC	3 cut(s)	1182, 1581, 1806
BcgI	CGANNNNNNTGC	2 cut(s)	1354, 1388
BciT130I	CCWGG	6 cut(s)	139, 941, 1108, 1115, 1123, 1517
BciVI	GTATCC	2 cut(s)	1513, 2910
BclI	TGATCA	1 cut(s)	2377
BcnI	CCSGG	4 cut(s)	288, 289, 1873, 1874
BcoDI	GTCTC	7 cut(s)	161, 444, 584, 1066, 2138, 2214, 3039
BfaI	CTAG	6 cut(s)	1587, 2048, 2114, 2295, 2552, 2960
BfmI	CTRYAG	2 cut(s)	1758, 2268
BfrI	CTTAAG	2 cut(s)	1670, 3040
BfuAI	ACCTGC	1 cut(s)	1231
BfuI	GTATCC	2 cut(s)	1513, 2910
BglII	AGATCT	1 cut(s)	1864
BlpI	GCTNAGC	1 cut(s)	123
Bme18I	GGWCC	3 cut(s)	1205, 1479, 1615
BmeT110I	CYCGRG	4 cut(s)	177, 287, 1872, 2448
BmgBI	CACGTC	1 cut(s)	3049
BmgT120I	GGNCC	5 cut(s)	1205, 1479, 1513, 1615, 1819
BmiI	GGNNCC	9 cut(s)	143, 1112, 1374, 1481, 1500, 1644, 1820, 2334, 2485
BmsI	GCATC	8 cut(s)	412, 507, 1084, 1108, 1768, 2311, 2627, 2704
BpiI	GAAGAC	2 cut(s)	580, 2062
BpmI	CTGGAG	2 cut(s)	1129, 2124
Bpu10I	CCTNAGC	3 cut(s)	402, 524, 1128
Bpu1102I	GCTNAGC	1 cut(s)	123
Bpu14I	TTCGAA	1 cut(s)	58
BpuMI	CCSGG	4 cut(s)	288, 289, 1873, 1874
Bsa29I	ATCGAT	1 cut(s)	786
BsaHI	GRCGYC	1 cut(s)	1900
BsaWI	WCCGGW	2 cut(s)	271, 851
BsaXI	ACNNNNNCTCC	2 cut(s)	422, 452
Bsc4I	CCNNNNNNNGG	5 cut(s)	138, 278, 293, 2051, 3026
Bse1I	ACTGG	8 cut(s)	1379, 1391, 1500, 1502, 2307, 2633, 2663, 2885
Bse3DI	GCAATG	3 cut(s)	724, 2194, 2354
BseAI	TCCGGA	1 cut(s)	271
BseBI	CCWGG	6 cut(s)	139, 941, 1108, 1115, 1123, 1517
BseCI	ATCGAT	1 cut(s)	786
BseGI	GGATG	9 cut(s)	439, 522, 1123, 1424, 1783, 2023, 2182, 2326, 2908
BseLI	CCNNNNNNNGG	5 cut(s)	138, 278, 293, 2051, 3026
BseMI	GCAATG	3 cut(s)	724, 2194, 2354
BseMII	CTCAG	3 cut(s)	393, 515, 2295
BseNI	ACTGG	8 cut(s)	1379, 1391, 1500, 1502, 2307, 2633, 2663, 2885
BseRI	GAGGAG	4 cut(s)	443, 541, 1419, 2479
BseX3I	CGGCCG	1 cut(s)	1185
BseYI	CCCAGC	2 cut(s)	244, 1728
BsgI	GTGCAG	1 cut(s)	2556
Bsh1236I	CGCG	2 cut(s)	1983, 2481
Bsh1285I	CGRYCG	1 cut(s)	1188
BshFI	GGCC	4 cut(s)	1187, 1514, 1596, 1821
BshNI	GGYRCC	1 cut(s)	1372
BshVI	ATCGAT	1 cut(s)	786
BsiEI	CGRYCG	1 cut(s)	1188
BsiHKAI	GWGCWC	3 cut(s)	654, 918, 2449
BsiHKCI	CYCGRG	4 cut(s)	177, 287, 1872, 2448
BsiSI	CCGG	6 cut(s)	272, 288, 748, 852, 1184, 1873
BslFI	GGGAC	1 cut(s)	1911
BslI	CCNNNNNNNGG	5 cut(s)	138, 278, 293, 2051, 3026
BsmAI	GTCTC	7 cut(s)	161, 444, 584, 1066, 2138, 2214, 3039
BsmBI	CGTCTC	2 cut(s)	161, 3039
BsmFI	GGGAC	1 cut(s)	1911
BsmI	GAATGC	1 cut(s)	1723
BsnI	GGCC	4 cut(s)	1187, 1514, 1596, 1821
BsoBI	CYCGRG	4 cut(s)	177, 287, 1872, 2448
Bsp119I	TTCGAA	1 cut(s)	58
Bsp1286I	GDGCHC	3 cut(s)	654, 918, 2449
Bsp13I	TCCGGA	1 cut(s)	271
Bsp143I	GATC	9 cut(s)	787, 1189, 1258, 1864, 2080, 2332, 2377, 2452, 2535
Bsp1720I	GCTNAGC	1 cut(s)	123
Bsp68I	TCGCGA	1 cut(s)	1983
BspANI	GGCC	4 cut(s)	1187, 1514, 1596, 1821
BspCNI	CTCAG	3 cut(s)	394, 516, 2294
BspDI	ATCGAT	1 cut(s)	786
BspEI	TCCGGA	1 cut(s)	271
BspFNI	CGCG	2 cut(s)	1983, 2481
BspHI	TCATGA	3 cut(s)	1251, 2583, 2869
BspLI	GGNNCC	9 cut(s)	143, 1112, 1374, 1481, 1500, 1644, 1820, 2334, 2485
BspMAI	CTGCAG	1 cut(s)	2272
BspMI	ACCTGC	1 cut(s)	1231
BspPI	GGATC	5 cut(s)	1253, 2075, 2327, 2340, 2460
BspQI	GCTCTTC	2 cut(s)	2140, 2496
BspT104I	TTCGAA	1 cut(s)	58
BspT107I	GGYRCC	1 cut(s)	1372
BspTI	CTTAAG	2 cut(s)	1670, 3040
BsrBI	CCGCTC	2 cut(s)	2549, 2653
BsrDI	GCAATG	3 cut(s)	724, 2194, 2354
BsrI	ACTGG	8 cut(s)	1379, 1391, 1500, 1502, 2307, 2633, 2663, 2885
BssMI	GATC	9 cut(s)	787, 1189, 1258, 1864, 2080, 2332, 2377, 2452, 2535
BssNAI	GTATAC	1 cut(s)	2278
BssNI	GRCGYC	1 cut(s)	1900
BssSI	CACGAG	1 cut(s)	917
BssT1I	CCWWGG	4 cut(s)	204, 1484, 1906, 2212
Bst1107I	GTATAC	1 cut(s)	2278
Bst2BI	CACGAG	1 cut(s)	917
Bst2UI	CCWGG	6 cut(s)	139, 941, 1108, 1115, 1123, 1517
Bst4CI	ACNGT	7 cut(s)	168, 465, 1249, 1619, 1759, 2410, 2681
Bst6I	CTCTTC	4 cut(s)	563, 2140, 2212, 2496
BstACI	GRCGYC	1 cut(s)	1900
BstAFI	CTTAAG	2 cut(s)	1670, 3040
BstAPI	GCANNNNNTGC	2 cut(s)	1060, 2360
BstBI	TTCGAA	1 cut(s)	58
BstC8I	GCNNGC	9 cut(s)	129, 394, 549, 2268, 2361, 2591, 2693, 2848, 2889
BstDEI	CTNAG	5 cut(s)	123, 402, 524, 1128, 2281
BstF5I	GGATG	9 cut(s)	439, 522, 1123, 1424, 1783, 2023, 2182, 2326, 2908
BstFNI	CGCG	2 cut(s)	1983, 2481
BstHHI	GCGC	4 cut(s)	11, 380, 717, 1440
BstKTI	GATC	9 cut(s)	790, 1192, 1261, 1867, 2083, 2335, 2380, 2455, 2538
BstMAI	GTCTC	7 cut(s)	161, 444, 584, 1066, 2138, 2214, 3039
BstMBI	GATC	9 cut(s)	787, 1189, 1258, 1864, 2080, 2332, 2377, 2452, 2535
BstMCI	CGRYCG	1 cut(s)	1188
BstNI	CCWGG	6 cut(s)	139, 941, 1108, 1115, 1123, 1517
BstNSI	RCATGY	2 cut(s)	646, 2891
BstSFI	CTRYAG	2 cut(s)	1758, 2268
BstUI	CGCG	2 cut(s)	1983, 2481
BstV2I	GAAGAC	2 cut(s)	580, 2062
BstX2I	RGATCY	4 cut(s)	1258, 1864, 2080, 2332
BstXI	CCANNNNNNTGG	3 cut(s)	339, 1386, 2640
BstYI	RGATCY	4 cut(s)	1258, 1864, 2080, 2332
BstZ17I	GTATAC	1 cut(s)	2278
BstZI	CGGCCG	1 cut(s)	1185
Bsu15I	ATCGAT	1 cut(s)	786
BsuI	GTATCC	2 cut(s)	1513, 2910
BsuRI	GGCC	4 cut(s)	1187, 1514, 1596, 1821
BsuTUI	ATCGAT	1 cut(s)	786
BtrI	CACGTC	1 cut(s)	3049
BtsCI	GGATG	9 cut(s)	439, 522, 1123, 1424, 1783, 2023, 2182, 2326, 2908
BtsI	GCAGTG	1 cut(s)	2106
BtsIMutI	CAGTG	5 cut(s)	417, 563, 2096, 2106, 2415
BtuMI	TCGCGA	1 cut(s)	1983
BveI	ACCTGC	1 cut(s)	1231
Cac8I	GCNNGC	9 cut(s)	129, 394, 549, 2268, 2361, 2591, 2693, 2848, 2889
CaiI	CAGNNNCTG	2 cut(s)	1652, 2240
CciI	TCATGA	3 cut(s)	1251, 2583, 2869
CfoI	GCGC	4 cut(s)	11, 380, 717, 1440
Cfr13I	GGNCC	5 cut(s)	1205, 1479, 1513, 1615, 1819
Cfr9I	CCCGGG	2 cut(s)	287, 1872
ClaI	ATCGAT	1 cut(s)	786
CseI	GACGC	4 cut(s)	385, 1908, 2067, 2893
Csp6I	GTAC	9 cut(s)	556, 622, 894, 1411, 1559, 2168, 2300, 2636, 2878
CviQI	GTAC	9 cut(s)	556, 622, 894, 1411, 1559, 2168, 2300, 2636, 2878
DdeI	CTNAG	5 cut(s)	123, 402, 524, 1128, 2281
DpnI	GATC	9 cut(s)	789, 1191, 1260, 1866, 2082, 2334, 2379, 2454, 2537
DpnII	GATC	9 cut(s)	787, 1189, 1258, 1864, 2080, 2332, 2377, 2452, 2535
DraIII	CACNNNGTG	1 cut(s)	1348
EaeI	YGGCCR	1 cut(s)	1185
EagI	CGGCCG	1 cut(s)	1185
Eam1104I	CTCTTC	4 cut(s)	563, 2140, 2212, 2496
EarI	CTCTTC	4 cut(s)	563, 2140, 2212, 2496
EciI	GGCGGA	1 cut(s)	529
EclXI	CGGCCG	1 cut(s)	1185
Eco130I	CCWWGG	4 cut(s)	204, 1484, 1906, 2212
Eco32I	GATATC	2 cut(s)	96, 2308
Eco47I	GGWCC	3 cut(s)	1205, 1479, 1615
Eco52I	CGGCCG	1 cut(s)	1185
Eco57I	CTGAAG	5 cut(s)	587, 615, 1674, 1952, 2139
Eco88I	CYCGRG	4 cut(s)	177, 287, 1872, 2448
EcoO109I	RGGNCCY	2 cut(s)	1479, 1513
EcoRII	CCWGG	6 cut(s)	137, 939, 1106, 1113, 1121, 1515
EcoRV	GATATC	2 cut(s)	96, 2308
EcoT14I	CCWWGG	4 cut(s)	204, 1484, 1906, 2212
EcoT22I	ATGCAT	1 cut(s)	828
ErhI	CCWWGG	4 cut(s)	204, 1484, 1906, 2212
Esp3I	CGTCTC	2 cut(s)	161, 3039
FalI	AAGNNNNNCTT	6 cut(s)	1904, 1936, 1925, 1957, 2525, 2557
FaqI	GGGAC	1 cut(s)	1911
FauI	CCCGC	1 cut(s)	228
FbaI	TGATCA	1 cut(s)	2377
FblI	GTMKAC	2 cut(s)	1968, 2277
FokI	GGATG	9 cut(s)	446, 529, 1130, 1411, 1790, 2030, 2189, 2333, 2915
FspAI	RTGCGCAY	1 cut(s)	1439
FspBI	CTAG	6 cut(s)	1587, 2048, 2114, 2295, 2552, 2960
FspI	TGCGCA	2 cut(s)	716, 1439
GlaI	GCGC	4 cut(s)	10, 379, 716, 1439
GsaI	CCCAGC	2 cut(s)	248, 1732
GsuI	CTGGAG	2 cut(s)	1129, 2124
HaeIII	GGCC	4 cut(s)	1187, 1514, 1596, 1821
HapII	CCGG	6 cut(s)	272, 288, 748, 852, 1184, 1873
HgaI	GACGC	4 cut(s)	385, 1908, 2067, 2893
HhaI	GCGC	4 cut(s)	11, 380, 717, 1440
Hin1I	GRCGYC	1 cut(s)	1900
Hin6I	GCGC	4 cut(s)	9, 378, 715, 1438
HinP1I	GCGC	4 cut(s)	9, 378, 715, 1438
HincII	GTYRAC	2 cut(s)	967, 1384
HindII	GTYRAC	2 cut(s)	967, 1384
HindIII	AAGCTT	1 cut(s)	2121
HpaII	CCGG	6 cut(s)	272, 288, 748, 852, 1184, 1873
HphI	GGTGA	9 cut(s)	308, 914, 1081, 1702, 2410, 2530, 2567, 3041, 3051
Hpy166II	GTNNAC	8 cut(s)	967, 1208, 1384, 1615, 1969, 2038, 2195, 2278
Hpy188I	TCNGA	6 cut(s)	994, 1146, 1864, 2494, 2623, 2657
Hpy8I	GTNNAC	8 cut(s)	967, 1208, 1384, 1615, 1969, 2038, 2195, 2278
Hpy99I	CGWCG	1 cut(s)	401
HpyCH4III	ACNGT	7 cut(s)	168, 465, 1249, 1619, 1759, 2410, 2681
HpyCH4IV	ACGT	3 cut(s)	1271, 1924, 3048
HpyF3I	CTNAG	5 cut(s)	123, 402, 524, 1128, 2281
HpySE526I	ACGT	3 cut(s)	1271, 1924, 3048
Hsp92I	GRCGYC	1 cut(s)	1900
HspAI	GCGC	4 cut(s)	9, 378, 715, 1438
Kpn2I	TCCGGA	1 cut(s)	271
Ksp22I	TGATCA	1 cut(s)	2377
Kzo9I	GATC	9 cut(s)	787, 1189, 1258, 1864, 2080, 2332, 2377, 2452, 2535
LguI	GCTCTTC	2 cut(s)	2140, 2496
LmnI	GCTCC	7 cut(s)	628, 667, 1110, 1448, 1489, 1744, 2231
LweI	GCATC	8 cut(s)	412, 507, 1084, 1108, 1768, 2311, 2627, 2704
MaeI	CTAG	6 cut(s)	1587, 2048, 2114, 2295, 2552, 2960
MaeII	ACGT	3 cut(s)	1271, 1924, 3048
MaeIII	GTNAC	7 cut(s)	792, 1033, 1301, 1736, 2431, 3029, 3057
MalI	GATC	9 cut(s)	789, 1191, 1260, 1866, 2082, 2334, 2379, 2454, 2537
MbiI	CCGCTC	2 cut(s)	2549, 2653
MboI	GATC	9 cut(s)	787, 1189, 1258, 1864, 2080, 2332, 2377, 2452, 2535
MfeI	CAATTG	2 cut(s)	224, 357
MflI	RGATCY	4 cut(s)	1258, 1864, 2080, 2332
MhlI	GDGCHC	3 cut(s)	654, 918, 2449
MlyI	GAGTC	3 cut(s)	346, 2867, 3065
MmeI	TCCRAC	1 cut(s)	2952
Mph1103I	ATGCAT	1 cut(s)	828
MroI	TCCGGA	1 cut(s)	271
MroXI	GAANNNNTTC	1 cut(s)	2134
MslI	CAYNNNNRTG	5 cut(s)	2016, 2089, 2175, 2819, 2892
MspA1I	CMGCKG	3 cut(s)	244, 800, 887
MspCI	CTTAAG	2 cut(s)	1670, 3040
MspI	CCGG	6 cut(s)	272, 288, 748, 852, 1184, 1873
MunI	CAATTG	2 cut(s)	224, 357
Mva1269I	GAATGC	1 cut(s)	1723
MvaI	CCWGG	6 cut(s)	139, 941, 1108, 1115, 1123, 1517
MvnI	CGCG	2 cut(s)	1983, 2481
NciI	CCSGG	4 cut(s)	288, 289, 1873, 1874
NdeII	GATC	9 cut(s)	787, 1189, 1258, 1864, 2080, 2332, 2377, 2452, 2535
NlaIV	GGNNCC	9 cut(s)	143, 1112, 1374, 1481, 1500, 1644, 1820, 2334, 2485
NmuCI	GTSAC	6 cut(s)	1033, 1301, 1736, 2431, 3029, 3057
NruI	TCGCGA	1 cut(s)	1983
NsbI	TGCGCA	2 cut(s)	716, 1439
NsiI	ATGCAT	1 cut(s)	828
NspI	RCATGY	2 cut(s)	646, 2891
NspV	TTCGAA	1 cut(s)	58
PaeI	GCATGC	1 cut(s)	2891
PaeR7I	CTCGAG	1 cut(s)	177
PagI	TCATGA	3 cut(s)	1251, 2583, 2869
PciI	ACATGT	1 cut(s)	642
PciSI	GCTCTTC	2 cut(s)	2140, 2496
PcsI	WCGNNNNNNNCGW	1 cut(s)	1987
PctI	GAATGC	1 cut(s)	1723
PdmI	GAANNNNTTC	1 cut(s)	2134
PfeI	GAWTC	7 cut(s)	60, 209, 1159, 1351, 2008, 2030, 2950
PfoI	TCCNGGA	1 cut(s)	939
PleI	GAGTC	3 cut(s)	346, 2867, 3064
PpsI	GAGTC	3 cut(s)	346, 2867, 3064
PpuMI	RGGWCCY	1 cut(s)	1479
PscI	ACATGT	1 cut(s)	642
Psp5II	RGGWCCY	1 cut(s)	1479
Psp6I	CCWGG	6 cut(s)	137, 939, 1106, 1113, 1121, 1515
PspFI	CCCAGC	2 cut(s)	244, 1728
PspGI	CCWGG	6 cut(s)	137, 939, 1106, 1113, 1121, 1515
PspN4I	GGNNCC	9 cut(s)	143, 1112, 1374, 1481, 1500, 1644, 1820, 2334, 2485
PspPI	GGNCC	5 cut(s)	1205, 1479, 1513, 1615, 1819
PspPPI	RGGWCCY	1 cut(s)	1479
PsrI	GAACNNNNNNTAC	2 cut(s)	438, 470
PstI	CTGCAG	1 cut(s)	2272
PstNI	CAGNNNCTG	2 cut(s)	1652, 2240
PsuI	RGATCY	4 cut(s)	1258, 1864, 2080, 2332
PvuII	CAGCTG	3 cut(s)	244, 800, 887
RruI	TCGCGA	1 cut(s)	1983
RsaI	GTAC	9 cut(s)	557, 623, 895, 1412, 1560, 2169, 2301, 2637, 2879
RsaNI	GTAC	9 cut(s)	556, 622, 894, 1411, 1559, 2168, 2300, 2636, 2878
RseI	CAYNNNNRTG	5 cut(s)	2016, 2089, 2175, 2819, 2892
SapI	GCTCTTC	2 cut(s)	2140, 2496
Sau3AI	GATC	9 cut(s)	787, 1189, 1258, 1864, 2080, 2332, 2377, 2452, 2535
Sau96I	GGNCC	5 cut(s)	1205, 1479, 1513, 1615, 1819
SchI	GAGTC	3 cut(s)	346, 2867, 3065
SduI	GDGCHC	3 cut(s)	654, 918, 2449
SfaNI	GCATC	8 cut(s)	412, 507, 1084, 1108, 1768, 2311, 2627, 2704
SfcI	CTRYAG	2 cut(s)	1758, 2268
Sfr274I	CTCGAG	1 cut(s)	177
SfuI	TTCGAA	1 cut(s)	58
SinI	GGWCC	3 cut(s)	1205, 1479, 1615
SlaI	CTCGAG	1 cut(s)	177
SmaI	CCCGGG	2 cut(s)	289, 1874
SmiMI	CAYNNNNRTG	5 cut(s)	2016, 2089, 2175, 2819, 2892
SmlI	CTYRAG	3 cut(s)	177, 1670, 3040
SmoI	CTYRAG	3 cut(s)	177, 1670, 3040
SphI	GCATGC	1 cut(s)	2891
SspMI	CTAG	6 cut(s)	1587, 2048, 2114, 2295, 2552, 2960
StyI	CCWWGG	4 cut(s)	204, 1484, 1906, 2212
TaaI	ACNGT	7 cut(s)	168, 465, 1249, 1619, 1759, 2410, 2681
TaiI	ACGT	3 cut(s)	1274, 1927, 3051
TaqI	TCGA	6 cut(s)	58, 178, 786, 1990, 2802, 2937
TatI	WGTACW	1 cut(s)	621
TauI	GCSGC	2 cut(s)	514, 523
TfiI	GAWTC	7 cut(s)	60, 209, 1159, 1351, 2008, 2030, 2950
TscAI	CASTG	5 cut(s)	424, 570, 2096, 2106, 2415
TseFI	GTSAC	6 cut(s)	1033, 1301, 1736, 2431, 3029, 3057
Tsp45I	GTSAC	6 cut(s)	1033, 1301, 1736, 2431, 3029, 3057
TspGWI	ACGGA	1 cut(s)	961
TspMI	CCCGGG	2 cut(s)	287, 1872
TspRI	CASTG	5 cut(s)	424, 570, 2096, 2106, 2415
Vha464I	CTTAAG	2 cut(s)	1670, 3040
VpaK11BI	GGWCC	3 cut(s)	1205, 1479, 1615
XapI	RAATTY	7 cut(s)	34, 46, 300, 2684, 2785, 2930, 2965
XceI	RCATGY	2 cut(s)	646, 2891
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1383
XhoI	CTCGAG	1 cut(s)	177
XmaI	CCCGGG	2 cut(s)	287, 1872
XmiI	GTMKAC	2 cut(s)	1968, 2277
XmnI	GAANNNNTTC	1 cut(s)	2134
XspI	CTAG	6 cut(s)	1587, 2048, 2114, 2295, 2552, 2960
Zsp2I	ATGCAT	1 cut(s)	828

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.