Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0003739

Physical Location & Seq

Genomic Coordinates Forward (+)

8657529 .. 8666148

Gene Structure 8620 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00086480.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2880 bp

ATGATATCAACCGCTGTGTGGAGAAGCTTCGCTAAACAAGCCATTTCCAGAGCCCAGAGGCTCCCCCTTAAACCCCAATCTTTTTCACGAGCTCATACCCTTCTGGGTTTTTGCCAAAGATCGAGTTTTCATGAAAATTTCAAGCCCATTTTGCTAAGTTCTTGCTCAAATTTTGTGGGTTTTCACCGAAATGATCATATGCACAAGCCCAGTTTTGGGTCTCTGAATCGAGTTGGGGTCAGTGATTTTTGCCCCAAAGGGTATGCTAGTGTAGCTGAGGCGGTTTCTTCCACTGATGCTGAGGAAGAGGTGTCTGTTGTTGATGGGCTTCAAGAAATATTGCAGGAGATGAGGAAAGAGGATAGGAGGCAGGCACAGTATAGGCGGCGGAGCCGGCAAGCTAGGCAGAGAGGAATGGGGCAAGGGAAGTACCTAATGCTTAAGAGGAAGCAGATAAAGATTGAGACCGAAGCGTGGGAGAAGGCCGCGAAAGAGTACAGGGAGCTTTTGATGGATATGTGTGAGCAGAAGCTGGCACCCAATTTGCCATATATGAAGTCTTTGTTCCTTGGTTGGTTTGAGCCTTTGTGTAATGCCATTACCAAGGAGCAAGACTTGTGCCGCCAAGGCAAGAATAAGGCGGCTTATGGTCCATATATGGATAAGTTACCGGCTGATATGATGTCGGTTATTACAATGCATAAGTTGATGGGGTTGCTGATGACCGGCGAGCATGGTAGTTGCAGGGTTGTATCGGCTGCTTGCACCATTGGTGAAGCCATAGAGCAGGAGGTTAGAATACACAACTTCTTGGAAAGGACAAAAAAGAAAAAGGTCGGTAAAGATCAGGAAACCGGAGAATCTGACGCTATGATGAAGGAAAATGAGAAGCTGAATAAAAAGGTCACAAATTTGATGAAAAAACAAAAAATGCAAGCAGTGAGGCACATAGTGAGGGGACATGATGATTCCAAAGGCTGGAGCCAGGAAGCAAAGGCTAAGGTTGGCAGTCGTTTGATTGAATTGCTGACCCAAACAGCTTTTATACAACCTCCGGCTAATCAGTTAGGGGACGGTCCGCTGGACATACGACCTGCATTTGTGCACACATTTAGGACTGTGAGCAAAGATGCAAAGAACAGTAGCAGAAGATATGGTGTTATTGAATGTGATCCTCTCGTTCTCAAAGGGCTTGAGAGAACAGTAAGTGATTTGGTGCTAAGCAAGGCACATGGTGATTCCTTATATGCCCATGTTGGTACCCCCACTCAATTGGACAGGGGTCTTTATGACAAAGGGGGGCACTTTTTCCTACCATCATATGTTATGCGCATTCATGGAGCTAAACAGCAGCGTGAAGCAGCCCTGGACACTCTTGGGAATACCAGATGGAGGGTTAATAAGAGGGTACTTAATGTTGTAGATAGGATATGGGCTAGTGGAGGCCGTCTTGCTGATTTGGTGGACCGCAGTGATGTTCCTTTACCGGAGAAACCAGATACTGAAGATGAAACATTGCTTAAAAAATGGAAGTGGAAAGTGACCTCTGTGAAGAAAGAGAACAGGGAGAGACATGCACAGCGTTGTGACATAGAACTTAAACTTGCTGTAGCACGGAAAATGAAGGATGAAGAAGGATTTTACTTTCCACACAATCTTGACTTCCGGGGCCGTGCATATCCTATGCACCCATACTTGAACCATCTCGGTTCAGATCTTTGCCGGGGTATTTTAGAGTTTGCCGAGGGACGCCCTCTTGGAAAGTCAGGCTTACGTTGGCTGAAGATACACTTGGCAAATCTATATGCTGGTGGTGTGGACAAATTATCTCTTGAGGGTCGACTAGCATTTACTGAGAATCATTTGGATGATATATTTGATTCTGTGGACAGACCACTAGAAGGAAGGCGCTGGTGGTTGAAGGCAGAAGATCCATTTCAGTGCTTGGCTGTGTGCGTTAACCTCTCTGAAGCTCTAAGAAGCTCTTCTCCAGAGACATTTATCTCACATATTCCTGTACATCAGGATGGCTCTTGCAATGGCTTACAACACTATGCTGCCCTTGGAAGGGACAAGTTAGGAGCAGGTGCTGTCAATCTGGTTGCAGGAGAGAAACCTGCTGATGTTTACTCAGGAATAGCTGCTAGGGTTCTGGCTATCATGCAGAGTGATGCACAGAAGGATCCTGAAGTTTTTCCTGATGCATTGCGTGCAAGAGTGTTAGTTAATCAGGTGGATAGGAAGTTGGTAAAGCAGACTGTGATGACGTCAGTATATGGTGTCACTTATATTGGTGCTCGGGATCAAATCAAGAGGAGGTTGAAGGAACGTGGTGCCATTTCAGACGATGCAGAGGTCTTTGGTTGTGCTTGTTATGCTGCAAAGGTCACCTTAACTGCTTTGGGGGAGATGTTTGAAGCTGCTCGAAGTATCATGAGCTGGCTTGGAGATTGCGCAAAAATAATTGCATCTGAAAATGAGCCAGTACGGTGGACAACTCCACTTGGACTTCCAGTTGTGCAACCTTACCGCAAATTTGGAAGGCATCTTATCATGGTCAAGAGGCAGAGGACAGCTTTCCCACCAAATTTTGTTCATTCCCTCGATGGTTCTCATATGATGATGACTGCAGTTGCCTGTAAAAAGGCAGGCTTAAACTTTGCAGGAGTTCATGACTCGTATTGGACACATGCTTGTGATGTGGATGTAATGAACAGGATACTCAGGGAAAAGTTTGTCGAGCTATATGAGACTCCAATACTAGAGAGATTGTCGGAGAGCTTTCAGCAGTCTTTTCCTGAATTAACTTTTCCGCCCTTACCTGAGCGGGGCGACTTTGATTTAAGGGACGTTTTAGAGTCACCTTATTTCTTTAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0000959 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006351 GO:0006390 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030880 GO:0032774 GO:0032991 GO:0034062 GO:0034245 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0061695 GO:0071704 GO:0090304 GO:0097659 GO:0097747 GO:0098798 GO:0140053 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RNA_pol RPOL_N

Protein Analysis

959

Amino Acids

108.58

Weight (kDa)

9.16

Isoelectric Point (pI)

49.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPOL_N	PF14700	151 - 402	2.5e-55	DNA-directed RNA polymerase N-terminal
RNA_pol	PF00940	584 - 933	5.3e-150	DNA-dependent RNA polymerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003739)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G15700 AT5G15700
fragaria_vesca	FvH4_6g46120 FvH4_6g46120 FvH4_6g46120 FvH4_6g46120 FvH4_6g46120 FvH4_6g46120 FvH4_6g46120 FvH4_6g46120
malus_domestica	MD09G1076800.v1.1 MD15G1148800.v1.1 MD17G1067300.v1.1
prunus_persica	Prupe.3G247300_v2.0.a1 Prupe.3G247300_v2.0.a1 Prupe.3G247300_v2.0.a1 Prupe.3G247300_v2.0.a1
pyrus_communis	pycom09g00400 pycom17g06760
rosa_chinensis	RchiOBHm_Chr2g0164571
rosa_laevigata	RLG00000021466
rosa_multiflora	Rmu_co8270321.1_g000001 Rmu_sc0020353.1_g000005 Rmu_ssc0000076.1_g000002
rosa_roxburghii	Rroxscaffold_2G00086480
rosa_rugosa	Rorug02G0509000 Rorug02G0509100 Rorug02G0509100 Rorug02G0509200.1
rosa_samantha	Rh2AG576800 Rh2BG587900 Rh2CG558700 Rh2DG598500
rosa_wichuraiana	Rw2G047890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2075
AatII	GACGTC	1 cut(s)	2270
Acc16I	TGCGCA	2 cut(s)	1331, 2455
Acc36I	ACCTGC	3 cut(s)	1102, 2075, 2125
Acc65I	GGTACC	1 cut(s)	1259
AccB1I	GGYRCC	3 cut(s)	535, 1259, 2333
AccB7I	CCANNNNNTGG	1 cut(s)	978
AccBSI	CCGCTC	1 cut(s)	2827
AccI	GTMKAC	1 cut(s)	1840
AccII	CGCG	1 cut(s)	488
AclWI	GGATC	5 cut(s)	1166, 1925, 2177, 2190, 2310
AcsI	RAATTY	5 cut(s)	136, 169, 910, 2534, 2587
AcuI	CTGAAG	4 cut(s)	1524, 1802, 1989, 2208
AcyI	GRCGYC	2 cut(s)	1750, 2267
AdeI	CACNNNGTG	2 cut(s)	952, 1235
AfaI	GTAC	6 cut(s)	431, 497, 1261, 1410, 2019, 2487
AflII	CTTAAG	1 cut(s)	440
AgsI	TTSAA	8 cut(s)	142, 332, 1022, 1166, 1699, 1921, 2323, 2417
AjnI	CCWGG	2 cut(s)	984, 1365
AjuI	GAANNNNNNNTTGG	2 cut(s)	1775, 1807
Alw21I	GWGCWC	3 cut(s)	94, 1107, 2299
Alw26I	GTCTC	5 cut(s)	225, 458, 1564, 1988, 2744
Alw44I	GTGCAC	1 cut(s)	1103
AlwI	GGATC	5 cut(s)	1166, 1925, 2177, 2190, 2310
AlwNI	CAGNNNCTG	3 cut(s)	532, 1502, 2090
Ama87I	CYCGRG	1 cut(s)	2298
AoxI	GGCC	3 cut(s)	483, 1444, 1669
ApaLI	GTGCAC	1 cut(s)	1103
ApeKI	GCWGC	7 cut(s)	758, 1351, 1361, 2057, 2141, 2378, 2420
ApoI	RAATTY	5 cut(s)	136, 169, 910, 2534, 2587
Asp700I	GAANNNNTTC	2 cut(s)	1984, 2193
Asp718I	GGTACC	1 cut(s)	1259
AspLEI	GCGC	3 cut(s)	1332, 1911, 2456
AspS9I	GGNCC	4 cut(s)	650, 1076, 1465, 1669
AsuC2I	CCSGG	2 cut(s)	1667, 1724
AsuHPI	GGTGA	5 cut(s)	176, 785, 1247, 2380, 2853
AvaI	CYCGRG	1 cut(s)	2298
AvaII	GGWCC	3 cut(s)	650, 1076, 1465
BaeGI	GKGCMC	2 cut(s)	1107, 1305
BamHI	GGATCC	1 cut(s)	2182
BanI	GGYRCC	3 cut(s)	535, 1259, 2333
BanII	GRGCYC	2 cut(s)	55, 94
BauI	CACGAG	1 cut(s)	87
Bbv12I	GWGCWC	3 cut(s)	94, 1107, 2299
BbvCI	CCTCAGC	2 cut(s)	276, 300
BbvI	GCAGC	7 cut(s)	745, 1363, 1373, 2044, 2128, 2365, 2407
BccI	CCATC	8 cut(s)	317, 505, 703, 1324, 1383, 1710, 2021, 2600
BceAI	ACGGC	2 cut(s)	1431, 1656
BciT130I	CCWGG	2 cut(s)	986, 1367
BciVI	GTATCC	1 cut(s)	2712
BclI	TGATCA	1 cut(s)	193
BcnI	CCSGG	2 cut(s)	1667, 1724
BcoDI	GTCTC	5 cut(s)	225, 458, 1564, 1988, 2744
BfaI	CTAG	7 cut(s)	267, 402, 1437, 1844, 1898, 2145, 2762
BfmI	CTRYAG	2 cut(s)	1608, 2628
BfoI	RGCGCY	1 cut(s)	1912
BfrI	CTTAAG	1 cut(s)	440
BfuAI	ACCTGC	3 cut(s)	1102, 2075, 2125
BfuI	GTATCC	1 cut(s)	2712
BglI	GCCNNNNNGGC	1 cut(s)	627
BglII	AGATCT	1 cut(s)	1714
BlpI	GCTNAGC	1 cut(s)	1220
Bme1390I	CCNGG	4 cut(s)	986, 1367, 1667, 1724
Bme18I	GGWCC	3 cut(s)	650, 1076, 1465
BmeT110I	CYCGRG	1 cut(s)	2298
BmgT120I	GGNCC	4 cut(s)	650, 1076, 1465, 1669
BmiI	GGNNCC	8 cut(s)	62, 392, 537, 983, 1261, 1670, 2184, 2335
BmrFI	CCNGG	4 cut(s)	986, 1367, 1667, 1724
BmrI	ACTGGG	1 cut(s)	204
BmsI	GCATC	7 cut(s)	286, 1120, 2161, 2191, 2338, 2477, 2554
BmuI	ACTGGG	1 cut(s)	204
BoxI	GACNNNNGTC	1 cut(s)	1281
BpmI	CTGGAG	2 cut(s)	1000, 1974
Bpu10I	CCTNAGC	4 cut(s)	276, 300, 999, 2823
Bpu1102I	GCTNAGC	1 cut(s)	1220
BpuEI	CTTGAG	2 cut(s)	1214, 1853
BpuMI	CCSGG	2 cut(s)	1667, 1724
BsaHI	GRCGYC	2 cut(s)	1750, 2267
BsaI	GGTCTC	2 cut(s)	225, 458
BsaJI	CCNNGG	8 cut(s)	568, 603, 625, 1365, 1666, 1723, 1743, 2062
BsaWI	WCCGGW	2 cut(s)	854, 1486
Bse118I	RCCGGY	3 cut(s)	393, 670, 725
Bse1I	ACTGG	3 cut(s)	210, 2483, 2513
Bse3DI	GCAATG	3 cut(s)	1514, 2044, 2204
BseBI	CCWGG	2 cut(s)	986, 1367
BseDI	CCNNGG	8 cut(s)	568, 603, 625, 1365, 1666, 1723, 1743, 2062
BseGI	GGATG	4 cut(s)	1633, 1873, 2032, 2710
BseMI	GCAATG	3 cut(s)	1514, 2044, 2204
BseMII	CTCAG	6 cut(s)	267, 291, 1845, 2145, 2737, 2814
BseNI	ACTGG	3 cut(s)	210, 2483, 2513
BseRI	GAGGAG	1 cut(s)	2329
BseSI	GKGCMC	2 cut(s)	1107, 1305
BseXI	GCAGC	7 cut(s)	745, 1363, 1373, 2044, 2128, 2365, 2407
Bsh1236I	CGCG	1 cut(s)	488
BshFI	GGCC	3 cut(s)	485, 1446, 1671
BshNI	GGYRCC	3 cut(s)	535, 1259, 2333
BsiHKAI	GWGCWC	3 cut(s)	94, 1107, 2299
BsiHKCI	CYCGRG	1 cut(s)	2298
BsiSI	CCGG	8 cut(s)	394, 671, 726, 855, 1055, 1487, 1666, 1723
BslFI	GGGAC	5 cut(s)	972, 1085, 1761, 2084, 2861
BsmAI	GTCTC	5 cut(s)	225, 458, 1564, 1988, 2744
BsmFI	GGGAC	5 cut(s)	972, 1085, 1761, 2084, 2861
BsmI	GAATGC	1 cut(s)	1332
BsnI	GGCC	3 cut(s)	485, 1446, 1671
Bso31I	GGTCTC	2 cut(s)	225, 458
BsoBI	CYCGRG	1 cut(s)	2298
Bsp1286I	GDGCHC	5 cut(s)	55, 94, 1107, 1305, 2299
Bsp1407I	TGTACA	1 cut(s)	2017
Bsp143I	GATC	8 cut(s)	119, 193, 844, 1171, 1714, 1930, 2182, 2302
Bsp1720I	GCTNAGC	1 cut(s)	1220
BspANI	GGCC	3 cut(s)	485, 1446, 1671
BspCNI	CTCAG	6 cut(s)	268, 292, 1846, 2144, 2736, 2815
BspFNI	CGCG	1 cut(s)	488
BspHI	TCATGA	3 cut(s)	130, 2433, 2671
BspLI	GGNNCC	8 cut(s)	62, 392, 537, 983, 1261, 1670, 2184, 2335
BspMAI	CTGCAG	1 cut(s)	2632
BspMI	ACCTGC	3 cut(s)	1102, 2075, 2125
BspPI	GGATC	5 cut(s)	1166, 1925, 2177, 2190, 2310
BspQI	GCTCTTC	1 cut(s)	1990
BspT107I	GGYRCC	3 cut(s)	535, 1259, 2333
BspTI	CTTAAG	1 cut(s)	440
BspTNI	GGTCTC	2 cut(s)	225, 458
BsrBI	CCGCTC	1 cut(s)	2827
BsrDI	GCAATG	3 cut(s)	1514, 2044, 2204
BsrFI	RCCGGY	3 cut(s)	393, 670, 725
BsrGI	TGTACA	1 cut(s)	2017
BsrI	ACTGG	3 cut(s)	210, 2483, 2513
BssAI	RCCGGY	3 cut(s)	393, 670, 725
BssECI	CCNNGG	8 cut(s)	568, 603, 625, 1365, 1666, 1723, 1743, 2062
BssMI	GATC	8 cut(s)	119, 193, 844, 1171, 1714, 1930, 2182, 2302
BssNI	GRCGYC	2 cut(s)	1750, 2267
BssSI	CACGAG	1 cut(s)	87
BssT1I	CCWWGG	4 cut(s)	568, 603, 625, 2062
Bst2BI	CACGAG	1 cut(s)	87
Bst2UI	CCWGG	2 cut(s)	986, 1367
Bst4CI	ACNGT	7 cut(s)	378, 1076, 1120, 1142, 1204, 2260, 2490
Bst6I	CTCTTC	2 cut(s)	300, 1990
BstACI	GRCGYC	2 cut(s)	1750, 2267
BstAFI	CTTAAG	1 cut(s)	440
BstAPI	GCANNNNNTGC	1 cut(s)	2210
BstAUI	TGTACA	1 cut(s)	2017
BstEII	GGTNACC	1 cut(s)	2386
BstF5I	GGATG	4 cut(s)	1633, 1873, 2032, 2710
BstFNI	CGCG	1 cut(s)	488
BstH2I	RGCGCY	1 cut(s)	1912
BstHHI	GCGC	3 cut(s)	1332, 1911, 2456
BstKTI	GATC	8 cut(s)	122, 196, 847, 1174, 1717, 1933, 2185, 2305
BstMAI	GTCTC	5 cut(s)	225, 458, 1564, 1988, 2744
BstMBI	GATC	8 cut(s)	119, 193, 844, 1171, 1714, 1930, 2182, 2302
BstMWI	GCNNNNNNNGC	8 cut(s)	38, 151, 272, 394, 403, 627, 2210, 2375
BstNI	CCWGG	2 cut(s)	986, 1367
BstNSI	RCATGY	2 cut(s)	1577, 2693
BstPAI	GACNNNNGTC	1 cut(s)	1281
BstPI	GGTNACC	1 cut(s)	2386
BstSCI	CCNGG	4 cut(s)	984, 1365, 1665, 1722
BstSFI	CTRYAG	2 cut(s)	1608, 2628
BstSLI	GKGCMC	2 cut(s)	1107, 1305
BstUI	CGCG	1 cut(s)	488
BstV1I	GCAGC	7 cut(s)	745, 1363, 1373, 2044, 2128, 2365, 2407
BstX2I	RGATCY	3 cut(s)	1714, 1930, 2182
BstXI	CCANNNNNNTGG	2 cut(s)	1273, 2490
BstYI	RGATCY	3 cut(s)	1714, 1930, 2182
BsuI	GTATCC	1 cut(s)	2712
BsuRI	GGCC	3 cut(s)	485, 1446, 1671
BtsCI	GGATG	4 cut(s)	1633, 1873, 2032, 2710
BtsI	GCAGTG	2 cut(s)	945, 1477
BtsIMutI	CAGTG	5 cut(s)	247, 291, 945, 1477, 1946
BveI	ACCTGC	3 cut(s)	1102, 2075, 2125
CaiI	CAGNNNCTG	3 cut(s)	532, 1502, 2090
CciI	TCATGA	3 cut(s)	130, 2433, 2671
CfoI	GCGC	3 cut(s)	1332, 1911, 2456
Cfr10I	RCCGGY	3 cut(s)	393, 670, 725
Cfr13I	GGNCC	4 cut(s)	650, 1076, 1465, 1669
CpoI	CGGWCCG	1 cut(s)	1076
CseI	GACGC	2 cut(s)	875, 1758
Csp6I	GTAC	6 cut(s)	430, 496, 1260, 1409, 2018, 2486
CspCI	CAANNNNNGTGG	2 cut(s)	156, 191
CspI	CGGWCCG	1 cut(s)	1076
CviQI	GTAC	6 cut(s)	430, 496, 1260, 1409, 2018, 2486
DpnI	GATC	8 cut(s)	121, 195, 846, 1173, 1716, 1932, 2184, 2304
DpnII	GATC	8 cut(s)	119, 193, 844, 1171, 1714, 1930, 2182, 2302
DraIII	CACNNNGTG	2 cut(s)	952, 1235
Eam1104I	CTCTTC	2 cut(s)	300, 1990
EarI	CTCTTC	2 cut(s)	300, 1990
EciI	GGCGGA	2 cut(s)	403, 2802
Ecl136II	GAGCTC	1 cut(s)	92
Eco130I	CCWWGG	4 cut(s)	568, 603, 625, 2062
Eco24I	GRGCYC	2 cut(s)	55, 94
Eco31I	GGTCTC	2 cut(s)	225, 458
Eco32I	GATATC	1 cut(s)	6
Eco47I	GGWCC	3 cut(s)	650, 1076, 1465
Eco53kI	GAGCTC	1 cut(s)	92
Eco57I	CTGAAG	4 cut(s)	1524, 1802, 1989, 2208
Eco88I	CYCGRG	1 cut(s)	2298
Eco91I	GGTNACC	1 cut(s)	2386
EcoICRI	GAGCTC	1 cut(s)	92
EcoO65I	GGTNACC	1 cut(s)	2386
EcoRII	CCWGG	2 cut(s)	984, 1365
EcoRV	GATATC	1 cut(s)	6
EcoT14I	CCWWGG	4 cut(s)	568, 603, 625, 2062
EcoT22I	ATGCAT	2 cut(s)	702, 2206
EcoT38I	GRGCYC	2 cut(s)	55, 94
ErhI	CCWWGG	4 cut(s)	568, 603, 625, 2062
FaqI	GGGAC	5 cut(s)	972, 1085, 1761, 2084, 2861
FauI	CCCGC	1 cut(s)	2820
FauNDI	CATATG	3 cut(s)	198, 1321, 2616
FbaI	TGATCA	1 cut(s)	193
FblI	GTMKAC	1 cut(s)	1840
FokI	GGATG	4 cut(s)	1640, 1880, 2039, 2717
FriOI	GRGCYC	2 cut(s)	55, 94
FspAI	RTGCGCAY	1 cut(s)	1331
FspBI	CTAG	7 cut(s)	267, 402, 1437, 1844, 1898, 2145, 2762
FspI	TGCGCA	2 cut(s)	1331, 2455
GlaI	GCGC	3 cut(s)	1331, 1910, 2455
GsuI	CTGGAG	2 cut(s)	1000, 1974
HaeII	RGCGCY	1 cut(s)	1912
HaeIII	GGCC	3 cut(s)	485, 1446, 1671
HapII	CCGG	8 cut(s)	394, 671, 726, 855, 1055, 1487, 1666, 1723
HgaI	GACGC	2 cut(s)	875, 1758
HhaI	GCGC	3 cut(s)	1332, 1911, 2456
Hin1I	GRCGYC	2 cut(s)	1750, 2267
Hin6I	GCGC	3 cut(s)	1330, 1909, 2454
HinP1I	GCGC	3 cut(s)	1330, 1909, 2454
HincII	GTYRAC	2 cut(s)	1841, 1960
HindII	GTYRAC	2 cut(s)	1841, 1960
HindIII	AAGCTT	1 cut(s)	25
HinfI	GANTC	9 cut(s)	226, 860, 968, 1238, 1858, 1880, 2675, 2752, 2858
HpaI	GTTAAC	1 cut(s)	1960
HpaII	CCGG	8 cut(s)	394, 671, 726, 855, 1055, 1487, 1666, 1723
HphI	GGTGA	5 cut(s)	176, 785, 1247, 2380, 2853
Hpy166II	GTNNAC	8 cut(s)	1105, 1465, 1819, 1841, 1888, 1960, 2128, 2493
Hpy188I	TCNGA	7 cut(s)	225, 865, 1714, 1969, 2344, 2473, 2776
Hpy8I	GTNNAC	8 cut(s)	1105, 1465, 1819, 1841, 1888, 1960, 2128, 2493
HpyCH4III	ACNGT	7 cut(s)	378, 1076, 1120, 1142, 1204, 2260, 2490
HpyCH4IV	ACGT	4 cut(s)	1774, 2267, 2329, 2850
HpyF10VI	GCNNNNNNNGC	8 cut(s)	38, 151, 272, 394, 403, 627, 2210, 2375
HpySE526I	ACGT	4 cut(s)	1774, 2267, 2329, 2850
Hsp92I	GRCGYC	2 cut(s)	1750, 2267
HspAI	GCGC	3 cut(s)	1330, 1909, 2454
KpnI	GGTACC	1 cut(s)	1263
KroI	GCCGGC	1 cut(s)	393
KroNI	GCCGGC	1 cut(s)	395
Ksp22I	TGATCA	1 cut(s)	193
KspAI	GTTAAC	1 cut(s)	1960
Kzo9I	GATC	8 cut(s)	119, 193, 844, 1171, 1714, 1930, 2182, 2302
LguI	GCTCTTC	1 cut(s)	1990
LmnI	GCTCC	7 cut(s)	66, 390, 502, 607, 981, 1340, 2081
Lsp1109I	GCAGC	7 cut(s)	745, 1363, 1373, 2044, 2128, 2365, 2407
LweI	GCATC	7 cut(s)	286, 1120, 2161, 2191, 2338, 2477, 2554
MaeI	CTAG	7 cut(s)	267, 402, 1437, 1844, 1898, 2145, 2762
MaeII	ACGT	4 cut(s)	1774, 2267, 2329, 2850
MaeIII	GTNAC	7 cut(s)	666, 904, 1540, 1586, 2281, 2386, 2859
MalI	GATC	8 cut(s)	121, 195, 846, 1173, 1716, 1932, 2184, 2304
MbiI	CCGCTC	1 cut(s)	2827
MboI	GATC	8 cut(s)	119, 193, 844, 1171, 1714, 1930, 2182, 2302
MboII	GAAGA	9 cut(s)	279, 317, 1161, 1517, 1564, 1643, 1795, 1940, 1977
MfeI	CAATTG	1 cut(s)	1271
MflI	RGATCY	3 cut(s)	1714, 1930, 2182
MhlI	GDGCHC	5 cut(s)	55, 94, 1107, 1305, 2299
MlyI	GAGTC	3 cut(s)	2669, 2746, 2867
MmeI	TCCRAC	1 cut(s)	2754
Mph1103I	ATGCAT	2 cut(s)	702, 2206
MroNI	GCCGGC	1 cut(s)	393
MroXI	GAANNNNTTC	2 cut(s)	1984, 2193
MslI	CAYNNNNRTG	5 cut(s)	189, 1866, 1939, 2025, 2694
MspA1I	CMGCKG	2 cut(s)	14, 1081
MspCI	CTTAAG	1 cut(s)	440
MspI	CCGG	8 cut(s)	394, 671, 726, 855, 1055, 1487, 1666, 1723
MspR9I	CCNGG	4 cut(s)	986, 1367, 1667, 1724
MunI	CAATTG	1 cut(s)	1271
Mva1269I	GAATGC	1 cut(s)	1332
MvaI	CCWGG	2 cut(s)	986, 1367
MvnI	CGCG	1 cut(s)	488
MwoI	GCNNNNNNNGC	8 cut(s)	38, 151, 272, 394, 403, 627, 2210, 2375
NaeI	GCCGGC	1 cut(s)	395
NciI	CCSGG	2 cut(s)	1667, 1724
NdeI	CATATG	3 cut(s)	198, 1321, 2616
NdeII	GATC	8 cut(s)	119, 193, 844, 1171, 1714, 1930, 2182, 2302
NgoMIV	GCCGGC	1 cut(s)	393
NlaIV	GGNNCC	8 cut(s)	62, 392, 537, 983, 1261, 1670, 2184, 2335
NmeAIII	GCCGAG	1 cut(s)	1768
NmuCI	GTSAC	6 cut(s)	904, 1540, 1586, 2281, 2386, 2859
NsbI	TGCGCA	2 cut(s)	1331, 2455
NsiI	ATGCAT	2 cut(s)	702, 2206
NspI	RCATGY	2 cut(s)	1577, 2693
PagI	TCATGA	3 cut(s)	130, 2433, 2671
PaqCI	CACCTGC	1 cut(s)	2075
PciSI	GCTCTTC	1 cut(s)	1990
PctI	GAATGC	1 cut(s)	1332
PdiI	GCCGGC	1 cut(s)	395
PdmI	GAANNNNTTC	2 cut(s)	1984, 2193
PfeI	GAWTC	6 cut(s)	226, 860, 968, 1238, 1858, 1880
PflMI	CCANNNNNTGG	1 cut(s)	978
PleI	GAGTC	3 cut(s)	2669, 2746, 2866
PpsI	GAGTC	3 cut(s)	2669, 2746, 2866
PshAI	GACNNNNGTC	1 cut(s)	1281
Psp124BI	GAGCTC	1 cut(s)	94
Psp6I	CCWGG	2 cut(s)	984, 1365
PspEI	GGTNACC	1 cut(s)	2386
PspGI	CCWGG	2 cut(s)	984, 1365
PspN4I	GGNNCC	8 cut(s)	62, 392, 537, 983, 1261, 1670, 2184, 2335
PspPI	GGNCC	4 cut(s)	650, 1076, 1465, 1669
PstI	CTGCAG	1 cut(s)	2632
PstNI	CAGNNNCTG	3 cut(s)	532, 1502, 2090
PsuI	RGATCY	3 cut(s)	1714, 1930, 2182
RsaI	GTAC	6 cut(s)	431, 497, 1261, 1410, 2019, 2487
RsaNI	GTAC	6 cut(s)	430, 496, 1260, 1409, 2018, 2486
RseI	CAYNNNNRTG	5 cut(s)	189, 1866, 1939, 2025, 2694
Rsr2I	CGGWCCG	1 cut(s)	1076
RsrII	CGGWCCG	1 cut(s)	1076
SacI	GAGCTC	1 cut(s)	94
SalI	GTCGAC	1 cut(s)	1839
SapI	GCTCTTC	1 cut(s)	1990
Sau3AI	GATC	8 cut(s)	119, 193, 844, 1171, 1714, 1930, 2182, 2302
Sau96I	GGNCC	4 cut(s)	650, 1076, 1465, 1669
SchI	GAGTC	3 cut(s)	2669, 2746, 2867
ScrFI	CCNGG	4 cut(s)	986, 1367, 1667, 1724
SduI	GDGCHC	5 cut(s)	55, 94, 1107, 1305, 2299
SfaNI	GCATC	7 cut(s)	286, 1120, 2161, 2191, 2338, 2477, 2554
SfcI	CTRYAG	2 cut(s)	1608, 2628
SinI	GGWCC	3 cut(s)	650, 1076, 1465
SmiMI	CAYNNNNRTG	5 cut(s)	189, 1866, 1939, 2025, 2694
SmlI	CTYRAG	3 cut(s)	440, 1193, 1832
SmoI	CTYRAG	3 cut(s)	440, 1193, 1832
SspI	AATATT	1 cut(s)	339
SspMI	CTAG	7 cut(s)	267, 402, 1437, 1844, 1898, 2145, 2762
SstI	GAGCTC	1 cut(s)	94
StyD4I	CCNGG	4 cut(s)	984, 1365, 1665, 1722
StyI	CCWWGG	4 cut(s)	568, 603, 625, 2062
TaaI	ACNGT	7 cut(s)	378, 1076, 1120, 1142, 1204, 2260, 2490
TaiI	ACGT	4 cut(s)	1777, 2270, 2332, 2853
TaqI	TCGA	6 cut(s)	122, 229, 1840, 2425, 2604, 2739
TaqII	GACCGA	1 cut(s)	482
TatI	WGTACW	2 cut(s)	495, 2017
TauI	GCSGC	4 cut(s)	388, 488, 624, 644
TfiI	GAWTC	6 cut(s)	226, 860, 968, 1238, 1858, 1880
TscAI	CASTG	5 cut(s)	247, 298, 945, 1477, 1946
TseFI	GTSAC	6 cut(s)	904, 1540, 1586, 2281, 2386, 2859
TseI	GCWGC	7 cut(s)	758, 1351, 1361, 2057, 2141, 2378, 2420
Tsp45I	GTSAC	6 cut(s)	904, 1540, 1586, 2281, 2386, 2859
TspGWI	ACGGA	1 cut(s)	1630
TspRI	CASTG	5 cut(s)	247, 298, 945, 1477, 1946
Van91I	CCANNNNNTGG	1 cut(s)	978
Vha464I	CTTAAG	1 cut(s)	440
VneI	GTGCAC	1 cut(s)	1103
VpaK11BI	GGWCC	3 cut(s)	650, 1076, 1465
XapI	RAATTY	5 cut(s)	136, 169, 910, 2534, 2587
XceI	RCATGY	2 cut(s)	1577, 2693
XmiI	GTMKAC	1 cut(s)	1840
XmnI	GAANNNNTTC	2 cut(s)	1984, 2193
XspI	CTAG	7 cut(s)	267, 402, 1437, 1844, 1898, 2145, 2762
ZraI	GACGTC	1 cut(s)	2268
Zsp2I	ATGCAT	2 cut(s)	702, 2206

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.