Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0006169

Physical Location & Seq

Genomic Coordinates Reverse (-)

7224365 .. 7226875

Gene Structure 2511 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1098900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2511 bp

ATGGATACCAAAACCAAGAAAAGATTGGTGCTTTCAGTTGTTTTCTTCTGCTTCCTCAAAACCCATGTCTCCCTTGCAGCCGACTCCATCACCGCCAACCAATCTCTCTCCGGTGATCAAACCATTGTCTCTGCCGGTGGGATTTTCGAGCTCGGTTTCTTCACACCAGGTAATTCTTCAAAGTTCTACGTAGGCATATGGTACACCAAGCAACTAGTATCCGAGAGGACCATAGTTTGGGTGGCAAACAGAGAAAAACCTGTTTCCGATCAATTTTCTTCGGTTTTAAGGATTTCAGATGGTAATTTAGTTCTCTTCAACGGGTCCAAAATCCCCGTTTGGTCAACGAACTTAACATCCTCCTCCGCCGCCACTGCACTTCTTTTGGATACCGGAAACCTGGTCTTGAGAGAGGGGTCTAGTAATAGTACGTCAGAGCCGTTATGGCAAAGCTTTGATCATCCGGCTCATACATGGCTGCCAGGAGCTAGAATTGGGTTTAACACTGTTACCAAACAAACCCAAATTCTTACTTCATGGAAGAGTTCGGAGGATCCTGCACCTGGTCTTTTCTCGCTCGAATTAGACCCGAAGGGGAGCCATGCGTATTTCATACGGTGGAATAGGTCTAGGCAGTATTGGAGTAGTGGACCATGGGATTCAAAATCAAAGCTTTTCAGCTGGGTTCCGGAAATGAGGCTCAATTATATATACAACTTCAGCTTTGTTACGAACGCAACCGAAAGTTACTTCACCTATTCTCTTTATGATCCAAAGATAATTTCTCGATTCGTGCTGAATACTTCGGGGCAGATTCAACAGCTGACATGGTTGGAGCCTTCCAGGCAATGGAATCTGTTTTGGTCTCAACCGAGACAACGATGCGAAGTTTATGATTTGTGCGGCGCCTTCGGAACTTGCAATGATGTAAGCACCGTCTCCTGTAATTGCTTGAACGGTTTTGAGCCAAAGGCGAAGAGAGATTGGGATTTGCAGGACTACTCTGGTGGGTGTAAGAGAAAAACCGATCTGCATTGTGAAAATGCTACTAGTGCGAGCGGGAAGCTAGACCGGTTTCTTGAACTTCCTAGCATGTCATTGCCTGAAAATGAACAATCTGTGGAGGTTGGGAATATTGCGGAATGTGAATCATTCTGCCTTAGAAACTGCTCTTGCACTGCTTATTCTTATAACGCAAGTGGATGTTCGATTTGGGAAGGAGAACTCTTGAATTTGCAACAACTAACATCAAGTGTCGACCACGGAATAACTTTATACCTCAGACTTGCAGCTTCTGAGTTTAAGAATTCTAAAAGTAACAACAAGGGATTGATTGTTGGTGTTGCGGCAGGCTCAGCTGCCGGCGTAGCAGTCCTCTTAGGCCTTATTGTGTTTGTAATCTTGGGACAGAGAAAGCGAGTGAATGGAATGGGAAAAGCAGTAGAGGGTTCGTTGATGGCATTCGGGTACAGAGAGATGCAAGATGCAACGAAGAACTTCTCCGAAAAATTGGGGGGAGGAGGTTTTGGTTCTGTTTTCAAAGGGACATTGCCTGATTCGTCTGTCATAGCAGTGAAGAAGCTTGAAAGTGTTAGCCAAGGGGAGAAGCAATTCAGAACAGAAGTGAGCACAATTGGGACCATCCAACACGTCAACCTTGTCCGCCTTCGCGGGTTCTGCTCTGAAGGTGCTAAAAGGATGTTGGTTTATGATTACATGCCGAATGGGTCTTTAGACTCTCACCTTTTCAACGATCAGCATCCGAATGTCTTGAATTGGAGAACAAGGTACCAAATTGCACTTGGGACAGCAAGAGGGTTGGCTTATCTTCACGAGAAATGCAGAGATTGCATCATACATTGTGACATAAAGCCGGAAAACATTCTCTTGGACGCTGAACTATGCCCCAAAGTGGCAGACTTTGGCCTTGCAAAGCTTGTTGGAAGAGAGTTCAGCAGGGTCCTGACTACCATGAGAGGGACAAGAGGCTATCTTGCGCCGGAGTGGATTTCGGGCGTGGCGATCACAGTGAAAGCGGATGTGTACAGCTACGGAATGATGCTGTTTGAATTCGTGTCGGGGAGAAGAAACTCCGAGCAATCTGAAGATGGGAAAGTTAGATTCTTCCCAACTTCTGCTGCTAGCAAGATTAGTAATCCAGAAAGCGATGTGCTGAGCCTATTAGACCCGAGGCTTGATGGCATTGCGGATGTAGAAGAGCTCACAAGGATTTGTAGAGTTGCTTGCTGGTGTGTCCAAGATGATGAGGCCCGGAGGCCGTCAATGGGGCAGGTGGTACAAATCCTCGAGGCGGTTTCCGATGTCAACTTGCCGCCAATTCCGAGAGCTCTTCAACTGTTAGGAGACGACCAGGAGCAGATAATCTTCTTCACTGAGTCATCCTCGAGCCAGAGTTCACGCCCACGGAGCAATAACAGATTAACGGCTTCCTCTCAAACCAAGAACTCCCCATCTTCAACCATCTCCAAGAGATCTGAAGAGCATCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

B_lectin PAN_2 Pkinase S_locus_glycop

Protein Analysis

837

Amino Acids

92.82

Weight (kDa)

7.73

Isoelectric Point (pI)

41.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	77 - 181	1e-32	D-mannose binding lectin
S_locus_glycop	PF00954	214 - 324	6.6e-32	S-locus glycoprotein domain
PAN_2	PF08276	355 - 411	1e-15	PAN-like domain
PK_Tyr_Ser-Thr	PF07714	500 - 767	2.2e-40	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	501 - 767	1.8e-46	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0006169)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G19130
fragaria_vesca	FvH4_6g44310
malus_domestica	MD09G1098900.v1.1 MD17G1087200.v1.1
prunus_persica	Prupe.3G227300_v2.0.a1 Prupe.3G227500_v2.0.a1
pyrus_communis	pycom09g02260 pycom17g08440
rosa_chinensis	RchiOBHm_Chr2g0161171 RchiOBHm_Chr2g0161191
rosa_laevigata	RLG00000021264 RLG00000021266 RLG00000029382
rosa_multiflora	Rmu_sc0006420.1_g000040 Rmu_sc0006420.1_g000045
rosa_roxburghii	Rroxscaffold_2G00089040 Rroxscaffold_2G00089060
rosa_rugosa	Rorug02G0486800 Rorug02G0486900
rosa_samantha	Rh2AG553500 Rh2BG567200 Rh2CG537300 Rh2CG537500 Rh2DG576500 Rh2DG576600
rosa_wichuraiana	Rw2G045780 Rw2G045800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1191
AarI	CACCTGC	1 cut(s)	2281
AbsI	CCTCGAGG	1 cut(s)	2306
Acc36I	ACCTGC	1 cut(s)	2281
Acc65I	GGTACC	1 cut(s)	1788
AccB1I	GGYRCC	2 cut(s)	905, 1788
AccB7I	CCANNNNNTGG	2 cut(s)	237, 849
AccBSI	CCGCTC	1 cut(s)	1059
AccI	GTMKAC	1 cut(s)	1257
AccII	CGCG	1 cut(s)	1671
AccIII	TCCGGA	1 cut(s)	688
AclWI	GGATC	3 cut(s)	548, 561, 764
AcsI	RAATTY	4 cut(s)	525, 1231, 1306, 2069
AcuI	CTGAAG	3 cut(s)	703, 1704, 2124
AcyI	GRCGYC	1 cut(s)	906
AfaI	GTAC	6 cut(s)	203, 430, 1469, 1790, 2045, 2298
AfiI	CCNNNNNNNGG	7 cut(s)	237, 563, 849, 1912, 1977, 2285, 2311
AflIII	ACRYGT	1 cut(s)	1648
AgeI	ACCGGT	1 cut(s)	1071
AhlI	ACTAGT	2 cut(s)	214, 1049
AjiI	CACGTC	1 cut(s)	1651
AjnI	CCWGG	6 cut(s)	166, 399, 481, 562, 842, 2370
AjuI	GAANNNNNNNTTGG	2 cut(s)	766, 798
AloI	GAACNNNNNNTCC	2 cut(s)	341, 373
Alw21I	GWGCWC	4 cut(s)	153, 1631, 2223, 2350
Alw26I	GTCTC	6 cut(s)	73, 133, 868, 870, 943, 2358
AlwI	GGATC	3 cut(s)	548, 561, 764
AlwNI	CAGNNNCTG	1 cut(s)	1295
Ama87I	CYCGRG	3 cut(s)	2188, 2306, 2404
Aor13HI	TCCGGA	1 cut(s)	688
AoxI	GGCC	4 cut(s)	1381, 1924, 2268, 2276
ApeKI	GCWGC	5 cut(s)	77, 478, 1289, 1358, 2138
ApoI	RAATTY	4 cut(s)	525, 1231, 1306, 2069
ArsI	GACNNNNNNTTYG	2 cut(s)	51, 83
AsiGI	ACCGGT	1 cut(s)	1071
Asp700I	GAANNNNTTC	3 cut(s)	1496, 1610, 1881
Asp718I	GGTACC	1 cut(s)	1788
AspLEI	GCGC	2 cut(s)	908, 1999
AspS9I	GGNCC	6 cut(s)	228, 324, 650, 1638, 1960, 2269
AsuC2I	CCSGG	1 cut(s)	2272
AsuHPI	GGTGA	4 cut(s)	82, 125, 745, 1733
AsuNHI	GCTAGC	1 cut(s)	2141
AvaI	CYCGRG	3 cut(s)	2188, 2306, 2404
AvaII	GGWCC	5 cut(s)	228, 324, 650, 1638, 1960
BamHI	GGATCC	1 cut(s)	553
BanI	GGYRCC	2 cut(s)	905, 1788
BanII	GRGCYC	3 cut(s)	153, 2223, 2350
BauI	CACGAG	1 cut(s)	1832
Bbv12I	GWGCWC	4 cut(s)	153, 1631, 2223, 2350
BbvI	GCAGC	5 cut(s)	89, 465, 1301, 1345, 2125
BccI	CCATC	8 cut(s)	95, 293, 1450, 1649, 2102, 2192, 2479, 2489
BceAI	ACGGC	3 cut(s)	424, 2263, 2460
BciT130I	CCWGG	6 cut(s)	168, 401, 483, 564, 844, 2372
BciVI	GTATCC	2 cut(s)	229, 382
BclI	TGATCA	2 cut(s)	115, 457
BcnI	CCSGG	1 cut(s)	2272
BcoDI	GTCTC	6 cut(s)	73, 133, 868, 870, 943, 2358
BcuI	ACTAGT	2 cut(s)	214, 1049
BfaI	CTAG	8 cut(s)	215, 420, 489, 630, 1050, 1067, 1089, 2142
BfoI	RGCGCY	1 cut(s)	909
BfuAI	ACCTGC	1 cut(s)	2281
BfuI	GTATCC	2 cut(s)	229, 382
BglI	GCCNNNNNGGC	2 cut(s)	445, 844
BglII	AGATCT	1 cut(s)	2492
BisI	GCNGC	9 cut(s)	78, 369, 479, 904, 1290, 1347, 1359, 2139, 2333
BlpI	GCTNAGC	2 cut(s)	1354, 2174
BlsI	GCNGC	9 cut(s)	79, 370, 480, 905, 1291, 1348, 1360, 2140, 2334
Bme1390I	CCNGG	7 cut(s)	168, 401, 483, 564, 844, 2272, 2372
Bme18I	GGWCC	5 cut(s)	228, 324, 650, 1638, 1960
BmeT110I	CYCGRG	3 cut(s)	2188, 2306, 2404
BmgBI	CACGTC	1 cut(s)	1651
BmgT120I	GGNCC	6 cut(s)	228, 324, 650, 1638, 1960, 2269
BmiI	GGNNCC	9 cut(s)	325, 555, 599, 687, 837, 907, 1639, 1790, 1961
BmrFI	CCNGG	7 cut(s)	168, 401, 483, 564, 844, 2272, 2372
BmsI	GCATC	6 cut(s)	872, 1467, 1474, 1768, 1860, 2049
BmtI	GCTAGC	1 cut(s)	2145
BplI	GAGNNNNNCTC	2 cut(s)	2387, 2419
Bpu1102I	GCTNAGC	2 cut(s)	1354, 2174
BpuEI	CTTGAG	1 cut(s)	427
BpuMI	CCSGG	1 cut(s)	2272
BsaAI	YACGTR	1 cut(s)	190
BsaBI	GATNNNNATC	1 cut(s)	1758
BsaHI	GRCGYC	1 cut(s)	906
BsaI	GGTCTC	1 cut(s)	870
BsaJI	CCNNGG	5 cut(s)	653, 1261, 1597, 2189, 2423
BsaWI	WCCGGW	4 cut(s)	110, 392, 688, 1071
Bsc4I	CCNNNNNNNGG	7 cut(s)	237, 563, 849, 1912, 1977, 2285, 2311
Bse118I	RCCGGY	3 cut(s)	134, 1071, 1361
Bse3DI	GCAATG	5 cut(s)	854, 928, 1097, 1547, 2202
Bse8I	GATNNNNATC	1 cut(s)	1758
BseAI	TCCGGA	1 cut(s)	688
BseBI	CCWGG	6 cut(s)	168, 401, 483, 564, 844, 2372
BseDI	CCNNGG	5 cut(s)	653, 1261, 1597, 2189, 2423
BseGI	GGATG	9 cut(s)	356, 460, 1208, 1641, 1704, 1759, 2044, 2215, 2399
BseJI	GATNNNNATC	1 cut(s)	1758
BseLI	CCNNNNNNNGG	7 cut(s)	237, 563, 849, 1912, 1977, 2285, 2311
BseMI	GCAATG	5 cut(s)	854, 928, 1097, 1547, 2202
BseMII	CTCAG	5 cut(s)	1287, 1294, 1368, 2165, 2385
BseRI	GAGGAG	2 cut(s)	352, 1533
BseXI	GCAGC	5 cut(s)	89, 465, 1301, 1345, 2125
BseYI	CCCAGC	1 cut(s)	681
BsgI	GTGCAG	2 cut(s)	360, 543
Bsh1236I	CGCG	1 cut(s)	1671
BshFI	GGCC	4 cut(s)	1383, 1926, 2270, 2278
BshNI	GGYRCC	2 cut(s)	905, 1788
BshTI	ACCGGT	1 cut(s)	1071
BsiHKAI	GWGCWC	4 cut(s)	153, 1631, 2223, 2350
BsiHKCI	CYCGRG	3 cut(s)	2188, 2306, 2404
BslFI	GGGAC	5 cut(s)	1419, 1558, 1651, 1819, 1993
BslI	CCNNNNNNNGG	7 cut(s)	237, 563, 849, 1912, 1977, 2285, 2311
BsmAI	GTCTC	6 cut(s)	73, 133, 868, 870, 943, 2358
BsmBI	CGTCTC	2 cut(s)	943, 2358
BsmFI	GGGAC	5 cut(s)	1419, 1558, 1651, 1819, 1993
BsmI	GAATGC	1 cut(s)	1460
BsnI	GGCC	4 cut(s)	1383, 1926, 2270, 2278
Bso31I	GGTCTC	1 cut(s)	870
BsoBI	CYCGRG	3 cut(s)	2188, 2306, 2404
Bsp1286I	GDGCHC	4 cut(s)	153, 1631, 2223, 2350
Bsp13I	TCCGGA	1 cut(s)	688
Bsp1407I	TGTACA	1 cut(s)	2043
Bsp143I	GATC	9 cut(s)	115, 268, 457, 553, 769, 1027, 1753, 2022, 2492
Bsp1720I	GCTNAGC	2 cut(s)	1354, 2174
Bsp19I	CCATGG	1 cut(s)	653
BspANI	GGCC	4 cut(s)	1383, 1926, 2270, 2278
BspCNI	CTCAG	5 cut(s)	1288, 1293, 1367, 2166, 2386
BspEI	TCCGGA	1 cut(s)	688
BspFNI	CGCG	1 cut(s)	1671
BspLI	GGNNCC	9 cut(s)	325, 555, 599, 687, 837, 907, 1639, 1790, 1961
BspMI	ACCTGC	1 cut(s)	2281
BspOI	GCTAGC	1 cut(s)	2145
BspPI	GGATC	3 cut(s)	548, 561, 764
BspQI	GCTCTTC	3 cut(s)	2211, 2355, 2493
BspT107I	GGYRCC	2 cut(s)	905, 1788
BspTNI	GGTCTC	1 cut(s)	870
BsrBI	CCGCTC	1 cut(s)	1059
BsrDI	GCAATG	5 cut(s)	854, 928, 1097, 1547, 2202
BsrFI	RCCGGY	3 cut(s)	134, 1071, 1361
BsrGI	TGTACA	1 cut(s)	2043
BssAI	RCCGGY	3 cut(s)	134, 1071, 1361
BssECI	CCNNGG	5 cut(s)	653, 1261, 1597, 2189, 2423
BssMI	GATC	9 cut(s)	115, 268, 457, 553, 769, 1027, 1753, 2022, 2492
BssNI	GRCGYC	1 cut(s)	906
BssSI	CACGAG	1 cut(s)	1832
BssT1I	CCWWGG	2 cut(s)	653, 1597
Bst2BI	CACGAG	1 cut(s)	1832
Bst2UI	CCWGG	6 cut(s)	168, 401, 483, 564, 844, 2372
Bst4CI	ACNGT	6 cut(s)	508, 618, 937, 959, 2029, 2358
Bst6I	CTCTTC	7 cut(s)	320, 536, 971, 1939, 2211, 2355, 2493
BstACI	GRCGYC	1 cut(s)	906
BstAPI	GCANNNNNTGC	1 cut(s)	1848
BstAUI	TGTACA	1 cut(s)	2043
BstBAI	YACGTR	1 cut(s)	190
BstC8I	GCNNGC	5 cut(s)	1057, 1351, 1363, 2143, 2245
BstDEI	CTNAG	7 cut(s)	1160, 1280, 1296, 1354, 1378, 2174, 2394
BstDSI	CCRYGG	3 cut(s)	653, 1261, 2423
BstF5I	GGATG	9 cut(s)	356, 460, 1208, 1641, 1704, 1759, 2044, 2215, 2399
BstFNI	CGCG	1 cut(s)	1671
BstH2I	RGCGCY	1 cut(s)	909
BstHHI	GCGC	2 cut(s)	908, 1999
BstKTI	GATC	9 cut(s)	118, 271, 460, 556, 772, 1030, 1756, 2025, 2495
BstMAI	GTCTC	6 cut(s)	73, 133, 868, 870, 943, 2358
BstMBI	GATC	9 cut(s)	115, 268, 457, 553, 769, 1027, 1753, 2022, 2492
BstMWI	GCNNNNNNNGC	9 cut(s)	374, 445, 844, 1052, 1355, 1367, 1677, 1848, 2427
BstNI	CCWGG	6 cut(s)	168, 401, 483, 564, 844, 2372
BstNSI	RCATGY	2 cut(s)	1096, 1720
BstSCI	CCNGG	7 cut(s)	166, 399, 481, 562, 842, 2270, 2370
BstSNI	TACGTA	1 cut(s)	190
BstUI	CGCG	1 cut(s)	1671
BstV1I	GCAGC	5 cut(s)	89, 465, 1301, 1345, 2125
BstX2I	RGATCY	2 cut(s)	553, 2492
BstYI	RGATCY	2 cut(s)	553, 2492
BsuI	GTATCC	2 cut(s)	229, 382
BsuRI	GGCC	4 cut(s)	1383, 1926, 2270, 2278
BtgI	CCRYGG	3 cut(s)	653, 1261, 2423
BtgZI	GCGATG	1 cut(s)	2181
BtrI	CACGTC	1 cut(s)	1651
BtsCI	GGATG	9 cut(s)	356, 460, 1208, 1641, 1704, 1759, 2044, 2215, 2399
BtsI	GCAGTG	3 cut(s)	372, 1176, 1578
BtsIMutI	CAGTG	6 cut(s)	372, 504, 1176, 1578, 2034, 2391
BveI	ACCTGC	1 cut(s)	2281
Cac8I	GCNNGC	5 cut(s)	1057, 1351, 1363, 2143, 2245
CaiI	CAGNNNCTG	1 cut(s)	1295
CfoI	GCGC	2 cut(s)	908, 1999
Cfr10I	RCCGGY	3 cut(s)	134, 1071, 1361
Cfr13I	GGNCC	6 cut(s)	228, 324, 650, 1638, 1960, 2269
CseI	GACGC	1 cut(s)	1901
CsiI	ACCWGGT	3 cut(s)	166, 399, 562
Csp6I	GTAC	6 cut(s)	202, 429, 1468, 1789, 2044, 2297
CspAI	ACCGGT	1 cut(s)	1071
CviAII	CATG	9 cut(s)	65, 474, 537, 602, 654, 828, 1093, 1717, 1972
CviQI	GTAC	6 cut(s)	202, 429, 1468, 1789, 2044, 2297
DdeI	CTNAG	7 cut(s)	1160, 1280, 1296, 1354, 1378, 2174, 2394
DinI	GGCGCC	1 cut(s)	907
DpnI	GATC	9 cut(s)	117, 270, 459, 555, 771, 1029, 1755, 2024, 2494
DpnII	GATC	9 cut(s)	115, 268, 457, 553, 769, 1027, 1753, 2022, 2492
Eam1104I	CTCTTC	7 cut(s)	320, 536, 971, 1939, 2211, 2355, 2493
EarI	CTCTTC	7 cut(s)	320, 536, 971, 1939, 2211, 2355, 2493
EciI	GGCGGA	2 cut(s)	355, 1652
Ecl136II	GAGCTC	3 cut(s)	151, 2221, 2348
Eco105I	TACGTA	1 cut(s)	190
Eco130I	CCWWGG	2 cut(s)	653, 1597
Eco147I	AGGCCT	1 cut(s)	1383
Eco24I	GRGCYC	3 cut(s)	153, 2223, 2350
Eco31I	GGTCTC	1 cut(s)	870
Eco47I	GGWCC	5 cut(s)	228, 324, 650, 1638, 1960
Eco53kI	GAGCTC	3 cut(s)	151, 2221, 2348
Eco57I	CTGAAG	3 cut(s)	703, 1704, 2124
Eco88I	CYCGRG	3 cut(s)	2188, 2306, 2404
EcoICRI	GAGCTC	3 cut(s)	151, 2221, 2348
EcoO109I	RGGNCCY	1 cut(s)	1960
EcoRI	GAATTC	2 cut(s)	1306, 2069
EcoRII	CCWGG	6 cut(s)	166, 399, 481, 562, 842, 2370
EcoT14I	CCWWGG	2 cut(s)	653, 1597
EcoT38I	GRGCYC	3 cut(s)	153, 2223, 2350
EgeI	GGCGCC	1 cut(s)	907
EheI	GGCGCC	1 cut(s)	907
ErhI	CCWWGG	2 cut(s)	653, 1597
Esp3I	CGTCTC	2 cut(s)	943, 2358
FaeI	CATG	9 cut(s)	68, 477, 540, 605, 657, 831, 1096, 1720, 1975
FaqI	GGGAC	5 cut(s)	1419, 1558, 1651, 1819, 1993
FatI	CATG	9 cut(s)	64, 473, 536, 601, 653, 827, 1092, 1716, 1971
FauI	CCCGC	2 cut(s)	1052, 1664
FauNDI	CATATG	1 cut(s)	197
FbaI	TGATCA	2 cut(s)	115, 457
FblI	GTMKAC	1 cut(s)	1257
Fnu4HI	GCNGC	9 cut(s)	78, 369, 479, 904, 1290, 1347, 1359, 2139, 2333
FokI	GGATG	9 cut(s)	343, 447, 1215, 1628, 1711, 1746, 2051, 2222, 2386
FriOI	GRGCYC	3 cut(s)	153, 2223, 2350
Fsp4HI	GCNGC	9 cut(s)	78, 369, 479, 904, 1290, 1347, 1359, 2139, 2333
FspBI	CTAG	8 cut(s)	215, 420, 489, 630, 1050, 1067, 1089, 2142
GlaI	GCGC	2 cut(s)	907, 1998
GluI	GCNGC	9 cut(s)	78, 369, 479, 904, 1290, 1347, 1359, 2139, 2333
GsaI	CCCAGC	1 cut(s)	685
HaeII	RGCGCY	1 cut(s)	909
HaeIII	GGCC	4 cut(s)	1383, 1926, 2270, 2278
HgaI	GACGC	1 cut(s)	1901
HhaI	GCGC	2 cut(s)	908, 1999
Hin1I	GRCGYC	1 cut(s)	906
Hin1II	CATG	9 cut(s)	68, 477, 540, 605, 657, 831, 1096, 1720, 1975
Hin6I	GCGC	2 cut(s)	906, 1997
HinP1I	GCGC	2 cut(s)	906, 1997
HincII	GTYRAC	4 cut(s)	345, 1258, 1654, 2326
HindII	GTYRAC	4 cut(s)	345, 1258, 1654, 2326
HindIII	AAGCTT	4 cut(s)	451, 671, 1580, 1934
HphI	GGTGA	4 cut(s)	82, 125, 745, 1733
Hpy166II	GTNNAC	8 cut(s)	204, 345, 650, 1258, 1654, 2044, 2326, 2417
Hpy188III	TCNNGA	9 cut(s)	406, 689, 786, 1079, 1228, 1771, 1832, 1963, 2159
Hpy8I	GTNNAC	8 cut(s)	204, 345, 650, 1258, 1654, 2044, 2326, 2417
HpyAV	CCTTC	6 cut(s)	586, 849, 919, 1211, 1676, 1679
HpyCH4III	ACNGT	6 cut(s)	508, 618, 937, 959, 2029, 2358
HpyCH4IV	ACGT	3 cut(s)	189, 431, 1650
HpyF10VI	GCNNNNNNNGC	9 cut(s)	374, 445, 844, 1052, 1355, 1367, 1677, 1848, 2427
HpyF3I	CTNAG	7 cut(s)	1160, 1280, 1296, 1354, 1378, 2174, 2394
HpySE526I	ACGT	3 cut(s)	189, 431, 1650
Hsp92I	GRCGYC	1 cut(s)	906
Hsp92II	CATG	9 cut(s)	68, 477, 540, 605, 657, 831, 1096, 1720, 1975
HspAI	GCGC	2 cut(s)	906, 1997
KasI	GGCGCC	1 cut(s)	905
Kpn2I	TCCGGA	1 cut(s)	688
KpnI	GGTACC	1 cut(s)	1792
KroI	GCCGGC	1 cut(s)	1361
KroNI	GCCGGC	1 cut(s)	1363
Ksp22I	TGATCA	2 cut(s)	115, 457
Kzo9I	GATC	9 cut(s)	115, 268, 457, 553, 769, 1027, 1753, 2022, 2492
LguI	GCTCTTC	3 cut(s)	2211, 2355, 2493
LmnI	GCTCC	5 cut(s)	485, 597, 835, 2374, 2427
Lsp1109I	GCAGC	5 cut(s)	89, 465, 1301, 1345, 2125
LweI	GCATC	6 cut(s)	872, 1467, 1474, 1768, 1860, 2049
MabI	ACCWGGT	3 cut(s)	166, 399, 562
MaeI	CTAG	8 cut(s)	215, 420, 489, 630, 1050, 1067, 1089, 2142
MaeII	ACGT	3 cut(s)	189, 431, 1650
MaeIII	GTNAC	5 cut(s)	508, 727, 746, 1316, 1862
MalI	GATC	9 cut(s)	117, 270, 459, 555, 771, 1029, 1755, 2024, 2494
MbiI	CCGCTC	1 cut(s)	1059
MboI	GATC	9 cut(s)	115, 268, 457, 553, 769, 1027, 1753, 2022, 2492
MfeI	CAATTG	1 cut(s)	1632
MflI	RGATCY	2 cut(s)	553, 2492
MhlI	GDGCHC	4 cut(s)	153, 1631, 2223, 2350
Mly113I	GGCGCC	1 cut(s)	906
MlyI	GAGTC	3 cut(s)	77, 1730, 2405
MmeI	TCCRAC	3 cut(s)	813, 1669, 1921
MroI	TCCGGA	1 cut(s)	688
MroNI	GCCGGC	1 cut(s)	1361
MroXI	GAANNNNTTC	3 cut(s)	1496, 1610, 1881
MseI	TTAA	5 cut(s)	287, 353, 501, 1302, 2441
MslI	CAYNNNNRTG	2 cut(s)	1571, 1764
MspA1I	CMGCKG	3 cut(s)	681, 823, 1358
MspR9I	CCNGG	7 cut(s)	168, 401, 483, 564, 844, 2272, 2372
MunI	CAATTG	1 cut(s)	1632
Mva1269I	GAATGC	1 cut(s)	1460
MvaI	CCWGG	6 cut(s)	168, 401, 483, 564, 844, 2372
MvnI	CGCG	1 cut(s)	1671
MwoI	GCNNNNNNNGC	9 cut(s)	374, 445, 844, 1052, 1355, 1367, 1677, 1848, 2427
NaeI	GCCGGC	1 cut(s)	1363
NarI	GGCGCC	1 cut(s)	906
NciI	CCSGG	1 cut(s)	2272
NcoI	CCATGG	1 cut(s)	653
NdeI	CATATG	1 cut(s)	197
NdeII	GATC	9 cut(s)	115, 268, 457, 553, 769, 1027, 1753, 2022, 2492
NgoMIV	GCCGGC	1 cut(s)	1361
NheI	GCTAGC	1 cut(s)	2141
NlaIII	CATG	9 cut(s)	68, 477, 540, 605, 657, 831, 1096, 1720, 1975
NlaIV	GGNNCC	9 cut(s)	325, 555, 599, 687, 837, 907, 1639, 1790, 1961
NmuCI	GTSAC	1 cut(s)	1862
NspI	RCATGY	2 cut(s)	1096, 1720
PaeR7I	CTCGAG	2 cut(s)	2306, 2404
PaqCI	CACCTGC	1 cut(s)	2281
PceI	AGGCCT	1 cut(s)	1383
PciSI	GCTCTTC	3 cut(s)	2211, 2355, 2493
PcsI	WCGNNNNNNNCGW	2 cut(s)	437, 2018
PctI	GAATGC	1 cut(s)	1460
PdiI	GCCGGC	1 cut(s)	1363
PdmI	GAANNNNTTC	3 cut(s)	1496, 1610, 1881
PfeI	GAWTC	7 cut(s)	659, 789, 814, 853, 1148, 1556, 2121
PflMI	CCANNNNNTGG	2 cut(s)	237, 849
PinAI	ACCGGT	1 cut(s)	1071
PkrI	GCNGC	9 cut(s)	79, 370, 480, 905, 1291, 1348, 1360, 2140, 2334
PleI	GAGTC	3 cut(s)	77, 1730, 2404
PluTI	GGCGCC	1 cut(s)	909
PpsI	GAGTC	3 cut(s)	77, 1730, 2404
Ppu21I	YACGTR	1 cut(s)	190
PpuMI	RGGWCCY	1 cut(s)	1960
PsiI	TTATAA	1 cut(s)	1191
Psp124BI	GAGCTC	3 cut(s)	153, 2223, 2350
Psp5II	RGGWCCY	1 cut(s)	1960
Psp6I	CCWGG	6 cut(s)	166, 399, 481, 562, 842, 2370
PspFI	CCCAGC	1 cut(s)	681
PspGI	CCWGG	6 cut(s)	166, 399, 481, 562, 842, 2370
PspN4I	GGNNCC	9 cut(s)	325, 555, 599, 687, 837, 907, 1639, 1790, 1961
PspPI	GGNCC	6 cut(s)	228, 324, 650, 1638, 1960, 2269
PspPPI	RGGWCCY	1 cut(s)	1960
PspXI	VCTCGAGB	2 cut(s)	2306, 2404
PstNI	CAGNNNCTG	1 cut(s)	1295
PsuI	RGATCY	2 cut(s)	553, 2492
PvuII	CAGCTG	3 cut(s)	681, 823, 1358
RsaI	GTAC	6 cut(s)	203, 430, 1469, 1790, 2045, 2298
RsaNI	GTAC	6 cut(s)	202, 429, 1468, 1789, 2044, 2297
RseI	CAYNNNNRTG	2 cut(s)	1571, 1764
SacI	GAGCTC	3 cut(s)	153, 2223, 2350
SalI	GTCGAC	1 cut(s)	1256
SapI	GCTCTTC	3 cut(s)	2211, 2355, 2493
SaqAI	TTAA	5 cut(s)	287, 353, 501, 1302, 2441
SatI	GCNGC	9 cut(s)	78, 369, 479, 904, 1290, 1347, 1359, 2139, 2333
Sau3AI	GATC	9 cut(s)	115, 268, 457, 553, 769, 1027, 1753, 2022, 2492
Sau96I	GGNCC	6 cut(s)	228, 324, 650, 1638, 1960, 2269
SchI	GAGTC	3 cut(s)	77, 1730, 2405
ScrFI	CCNGG	7 cut(s)	168, 401, 483, 564, 844, 2272, 2372
SduI	GDGCHC	4 cut(s)	153, 1631, 2223, 2350
SexAI	ACCWGGT	3 cut(s)	166, 399, 562
SfaNI	GCATC	6 cut(s)	872, 1467, 1474, 1768, 1860, 2049
SfoI	GGCGCC	1 cut(s)	907
Sfr274I	CTCGAG	2 cut(s)	2306, 2404
SinI	GGWCC	5 cut(s)	228, 324, 650, 1638, 1960
SlaI	CTCGAG	2 cut(s)	2306, 2404
SmiMI	CAYNNNNRTG	2 cut(s)	1571, 1764
SmlI	CTYRAG	3 cut(s)	406, 2306, 2404
SmoI	CTYRAG	3 cut(s)	406, 2306, 2404
SnaBI	TACGTA	1 cut(s)	190
SpeI	ACTAGT	2 cut(s)	214, 1049
SseBI	AGGCCT	1 cut(s)	1383
SspDI	GGCGCC	1 cut(s)	905
SspI	AATATT	1 cut(s)	1135
SspMI	CTAG	8 cut(s)	215, 420, 489, 630, 1050, 1067, 1089, 2142
SstI	GAGCTC	3 cut(s)	153, 2223, 2350
StuI	AGGCCT	1 cut(s)	1383
StyD4I	CCNGG	7 cut(s)	166, 399, 481, 562, 842, 2270, 2370
StyI	CCWWGG	2 cut(s)	653, 1597
TaaI	ACNGT	6 cut(s)	508, 618, 937, 959, 2029, 2358
TaiI	ACGT	3 cut(s)	192, 434, 1653
TaqI	TCGA	7 cut(s)	147, 579, 787, 1208, 1257, 2307, 2405
TatI	WGTACW	1 cut(s)	2043
TauI	GCSGC	4 cut(s)	371, 906, 1349, 2335
TfiI	GAWTC	7 cut(s)	659, 789, 814, 853, 1148, 1556, 2121
Tru1I	TTAA	5 cut(s)	287, 353, 501, 1302, 2441
Tru9I	TTAA	5 cut(s)	287, 353, 501, 1302, 2441
TscAI	CASTG	6 cut(s)	379, 511, 1183, 1578, 2034, 2398
TseFI	GTSAC	1 cut(s)	1862
TseI	GCWGC	5 cut(s)	77, 478, 1289, 1358, 2138
Tsp45I	GTSAC	1 cut(s)	1862
TspDTI	ATGAA	3 cut(s)	525, 601, 1125
TspGWI	ACGGA	3 cut(s)	1278, 2067, 2440
TspRI	CASTG	6 cut(s)	379, 511, 1183, 1578, 2034, 2398
Van91I	CCANNNNNTGG	2 cut(s)	237, 849
VpaK11BI	GGWCC	5 cut(s)	228, 324, 650, 1638, 1960
XapI	RAATTY	4 cut(s)	525, 1231, 1306, 2069
XceI	RCATGY	2 cut(s)	1096, 1720
XcmI	CCANNNNNNNNNTGG	3 cut(s)	22, 238, 1799
XhoI	CTCGAG	2 cut(s)	2306, 2404
XmiI	GTMKAC	1 cut(s)	1257
XmnI	GAANNNNTTC	3 cut(s)	1496, 1610, 1881
XspI	CTAG	8 cut(s)	215, 420, 489, 630, 1050, 1067, 1089, 2142

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.