Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2C

Orthogroup ID

OG0006169

Physical Location & Seq

Genomic Coordinates Forward (+)

71475532 .. 71478582

Gene Structure 3051 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2CG537500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2526 bp

ATGGAGTCCAAAACAAACCCAAAATTCTTGCTTTCCTTGCTTTTCTTAAGCCTATGCCTCAAGTCCCGTATCTGCTTTGGAGCTGACACCATCACAGCAAACCAGTCTCTCTCTGGTGACCAAACCATTGTTTCAGCTGGTGGGAAATTTGAGCTGGGTTTCTTCAAGGCAGGTAATACTTCAAACTACTACGTAGGCATGTGGTACTACAAGACAGTAGTACCAGAGCAAACCATAGTCTGGGTGGCCAATAGGGTGCAGCCAGTCTCTGATAGATTTTCTTCAGAGTTGAGAATCTCAGATGGTAATCTGGTGCTGTTTAATGAGTCCAAAACCCCAATTTGGTCCACAAATGTCAGTTCAAGTTCAGTTTCTTCTATACATGTAGTTCTTTCAGATAATGGAAACCTTGTGCTAAGAGCTGGGTCTAATTCATCTTTGCCTTTATGGCAAAGTTTTGATTATCCAGCTCATACTTGGCTACCAGGAGCTAGAATTGGGTTCAACACTGTTACAAACCAAACCCAAGTCCTGACTTCATGGAAGAATTCTGATGATCCTGCACCGGGTCTTTTCTCTCTTGAGCTTGACCCGAATGGAAGCGATGCATACTTCATACTGTGGAATAGGTCTATTCGGTATTGGACTAGTGGAGCTTGGGATCCAAAGAGTCGTATTTTCAGTTTGGTTCCTGAGATGAGGCTTAACTATATCTACAACTTCAGCTATGTTAAAAACAAAAATGAGAGCTACTTCACCTATTCTGTTTATGATCCTAGCATCACATCTCGATTCATAATGGATGTATCGGGTCAGATTAAGCAACGATCCTGGTTGAAATCTCAGGGATGGAACTTGTTTTGGTCTCAACCAAGGAAACAATGTGAGGTTTATGCTTATTGTGGGGCATTTGGCAGTTGCAATGAGAATTCTTTGCCCTTCTGTAATTGTTTGAATGGTTTTGAGCCGAAATTGAAGATGAATTGGGATTTGCAGGATTATTCTGGTGGCTGCAACAGACGGACTACGCTGAATTGTGGGAATGGTACCAATGGTACTACTGATAATGGAAAGAAACTGAAGAAAGACCGTTTTCGAGAACTGACTAGCATGTCATTGCCTGTGGGTGGTCAATCTGTGGAGGTTGGGAGTACTTCGCAATGTGAATCCTTTTGCTTCAATGATTGCAATTGCACTGCTTATGCATATGTCGACAATAAATGTTCAATTTGGTCTGGAGATCTCTTGGATCTGCAACAACTCACAGCAGATGACGGTAATGGAAAAACTTTGTACCTCAGACTTGCAGCTTCCGAGTTTAAGGATCCAAAAAGTAAAAAGGGATTGATTATCGGTGTTGCAGTAGGCTCAGCTGTTGGGATAGCAGTTCTTTTAGGCCTTATTGTGGTTGCAGTGTTGAGAGACAGAAACAGAGTGATTGGAAAATCAGTGGAGGGTTCATTGGTGGCATTTGAGTATAGAGATCTTCAAGAAGTAACAAAGAACTTCTCGGAGAAATTGGGGGGAGGAGGTTTTGGTTCTGTCTTCAAGGGCACAATGCCTGATTCATCAGTCATAGCAGTGAAGAAGCTCGAAAGTGTTAGCCAAGGAGAGAAGCAATTCAGAACAGAAGTCAGCACAATTGGGACAATCCAACATGTCAATCTTGTTCGGCTTCGTGGGTTCTGCTCTGCAGGTTCTAAGAGGTTGTTGGTATATGATTACATGCCTAATGGATCTCTGGATGCTCACCTTTTCAATTGTCCAAACGATGTTTTGGAGTGGAATACAAGATACCAGATTGCTCTGGGGACGGCAAGAGGGTTGGCTTATCTTCATGAGAAATGTAGAGATTGTATCATACATTGCGACATAAAGCCAGAAAACATTCTCCTAGATACTGAACTTTGTCCCAAAGTTGCAGACTTTGGCCTGGCAAAGCTTGTTGGAAGAGAGTTCAGCCGAGTCCTGACCACAATGAGAGGCACACGAGGCTATCTTGCACCAGAGTGGATTTCAGGAGTGGCAGTCACAGCAAAAGCTGATGTTTACAGCTACGGAATGATGCTCTTCGAAATTGTATCAGGAAGACGAAACTCTGAGCCATCTGAAGACGGCAAAGTTAGATTCTTCCCAAGTTGGGCTGCTGGCGTGATCACGAGTAACGAAGAAGGCGATGTGCTGAGCCTATTGGACCCGAAATTAGGGAGAAACGGTGATGTGGAAGAGATCATGAGGGTTCTTAGAGTTGCTTGCTGGTGTGTCCAAGATGATGAGGCTCATAGACCATCAATGGGTCAAGTGGTGCAGATTCTCGAGGGGGTTTTGAATGTGAACTTGCCCTCAGTTCCAAGATCACTCCAACTCTTTGGAGATGGCCAGGACATAGTTTTCTTCACAGAGTCATCTTCAAGCCAGAGCTCACATGCACCAAGTAATAACACATCAACAGCTTCATCTCAGATCAAGAGCGCCGCATCTTCAACTATCTCCAAGAGATCTGAAGATCATCAAACGAGCACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

B_lectin PAN_2 Pkinase S_locus_glycop

Protein Analysis

841

Amino Acids

92.97

Weight (kDa)

7.69

Isoelectric Point (pI)

36.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	78 - 182	6.5e-33	D-mannose binding lectin
S_locus_glycop	PF00954	215 - 324	4.5e-30	S-locus glycoprotein domain
PAN_2	PF08276	361 - 418	5.8e-13	PAN-like domain
PK_Tyr_Ser-Thr	PF07714	503 - 771	7.3e-40	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	504 - 770	8.8e-46	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0006169)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G19130
fragaria_vesca	FvH4_6g44310
malus_domestica	MD09G1098900.v1.1 MD17G1087200.v1.1
prunus_persica	Prupe.3G227300_v2.0.a1 Prupe.3G227500_v2.0.a1
pyrus_communis	pycom09g02260 pycom17g08440
rosa_chinensis	RchiOBHm_Chr2g0161171 RchiOBHm_Chr2g0161191
rosa_laevigata	RLG00000021264 RLG00000021266 RLG00000029382
rosa_multiflora	Rmu_sc0006420.1_g000040 Rmu_sc0006420.1_g000045
rosa_roxburghii	Rroxscaffold_2G00089040 Rroxscaffold_2G00089060
rosa_rugosa	Rorug02G0486800 Rorug02G0486900
rosa_samantha	Rh2AG553500 Rh2BG567200 Rh2CG537300 Rh2CG537500 Rh2DG576500 Rh2DG576600
rosa_wichuraiana	Rw2G045780 Rw2G045800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1111
Acc36I	ACCTGC	2 cut(s)	161, 1685
Acc65I	GGTACC	1 cut(s)	1046
AccB1I	GGYRCC	1 cut(s)	1046
AccB7I	CCANNNNNTGG	1 cut(s)	240
AccI	GTMKAC	1 cut(s)	1212
AciI	CCGC	1 cut(s)	2475
AclWI	GGATC	9 cut(s)	551, 656, 669, 767, 822, 1257, 1319, 1332, 1744
AcoI	YGGCCR	2 cut(s)	246, 2377
AcsI	RAATTY	4 cut(s)	23, 146, 547, 928
AcuI	CTGAAG	5 cut(s)	267, 706, 1100, 2130, 2523
AfaI	GTAC	6 cut(s)	206, 222, 1048, 1057, 1153, 1295
AfiI	CCNNNNNNNGG	8 cut(s)	240, 342, 566, 1127, 1405, 2140, 2204, 2294
AflII	CTTAAG	1 cut(s)	46
AflIII	ACRYGT	2 cut(s)	382, 1657
AhlI	ACTAGT	1 cut(s)	647
AjnI	CCWGG	4 cut(s)	484, 830, 1932, 2379
AleI	CACNNNNGTG	1 cut(s)	2008
Alw21I	GWGCWC	2 cut(s)	2423, 2522
Alw26I	GTCTC	4 cut(s)	111, 271, 870, 1416
AlwI	GGATC	9 cut(s)	551, 656, 669, 767, 822, 1257, 1319, 1332, 1744
AlwNI	CAGNNNCTG	1 cut(s)	269
Ama87I	CYCGRG	1 cut(s)	2315
AoxI	GGCC	4 cut(s)	246, 1396, 1930, 2377
ApeKI	GCWGC	4 cut(s)	259, 1011, 1307, 2144
ApoI	RAATTY	4 cut(s)	23, 146, 547, 928
Asp700I	GAANNNNTTC	2 cut(s)	1619, 1887
Asp718I	GGTACC	1 cut(s)	1046
AspLEI	GCGC	1 cut(s)	2474
AspS9I	GGNCC	2 cut(s)	345, 2194
AsuC2I	CCSGG	1 cut(s)	567
AsuHPI	GGTGA	4 cut(s)	128, 748, 1742, 2228
AsuII	TTCGAA	1 cut(s)	2073
AvaI	CYCGRG	1 cut(s)	2315
AvaII	GGWCC	2 cut(s)	345, 2194
BaeGI	GKGCMC	1 cut(s)	1556
BaeI	ACNNNNGTAYC	2 cut(s)	204, 237
BalI	TGGCCA	2 cut(s)	248, 2379
BamHI	GGATCC	2 cut(s)	661, 1324
BanI	GGYRCC	1 cut(s)	1046
BanII	GRGCYC	1 cut(s)	2423
BauI	CACGAG	2 cut(s)	1989, 2158
BbsI	GAAGAC	3 cut(s)	1537, 2095, 2118
Bbv12I	GWGCWC	2 cut(s)	2423, 2522
BbvI	GCAGC	4 cut(s)	271, 998, 1319, 2131
BccI	CCATC	6 cut(s)	98, 296, 843, 2113, 2296, 2369
BceAI	ACGGC	2 cut(s)	1830, 2131
BciT130I	CCWGG	4 cut(s)	486, 832, 1934, 2381
BclI	TGATCA	1 cut(s)	2154
BcnI	CCSGG	1 cut(s)	567
BcoDI	GTCTC	4 cut(s)	111, 271, 870, 1416
BcuI	ACTAGT	1 cut(s)	647
BfaI	CTAG	5 cut(s)	492, 648, 777, 1107, 1895
BfmI	CTRYAG	1 cut(s)	1692
BfoI	RGCGCY	1 cut(s)	2475
BfrI	CTTAAG	1 cut(s)	46
BfuAI	ACCTGC	2 cut(s)	161, 1685
BglI	GCCNNNNNGGC	1 cut(s)	448
BglII	AGATCT	3 cut(s)	1240, 1483, 2498
BisI	GCNGC	5 cut(s)	260, 1012, 1308, 2145, 2475
BlpI	GCTNAGC	2 cut(s)	1369, 2183
BlsI	GCNGC	5 cut(s)	261, 1013, 1309, 2146, 2476
BmcAI	AGTACT	1 cut(s)	1153
Bme1390I	CCNGG	5 cut(s)	486, 567, 832, 1934, 2381
Bme18I	GGWCC	2 cut(s)	345, 2194
BmeT110I	CYCGRG	1 cut(s)	2315
BmgT120I	GGNCC	2 cut(s)	345, 2194
BmiI	GGNNCC	5 cut(s)	663, 690, 1048, 1326, 2196
BmrFI	CCNGG	5 cut(s)	486, 567, 832, 1934, 2381
BmsI	GCATC	5 cut(s)	595, 789, 1735, 2055, 2486
BpiI	GAAGAC	3 cut(s)	1537, 2095, 2118
BpmI	CTGGAG	1 cut(s)	1257
Bpu1102I	GCTNAGC	2 cut(s)	1369, 2183
Bpu14I	TTCGAA	1 cut(s)	2073
BpuEI	CTTGAG	2 cut(s)	44, 602
BpuMI	CCSGG	1 cut(s)	567
BsaAI	YACGTR	1 cut(s)	193
BsaBI	GATNNNNATC	1 cut(s)	306
BsaI	GGTCTC	1 cut(s)	870
BsaJI	CCNNGG	2 cut(s)	872, 1606
BsaXI	ACNNNNNCTCC	4 cut(s)	2013, 2043, 2200, 2230
Bsc4I	CCNNNNNNNGG	8 cut(s)	240, 342, 566, 1127, 1405, 2140, 2204, 2294
Bse1I	ACTGG	2 cut(s)	103, 263
Bse3DI	GCAATG	4 cut(s)	928, 1115, 1166, 1864
Bse8I	GATNNNNATC	1 cut(s)	306
BseBI	CCWGG	4 cut(s)	486, 832, 1934, 2381
BseDI	CCNNGG	2 cut(s)	872, 1606
BseGI	GGATG	3 cut(s)	808, 854, 1750
BseJI	GATNNNNATC	1 cut(s)	306
BseLI	CCNNNNNNNGG	8 cut(s)	240, 342, 566, 1127, 1405, 2140, 2204, 2294
BseMI	GCAATG	4 cut(s)	928, 1115, 1166, 1864
BseMII	CTCAG	9 cut(s)	312, 684, 857, 1312, 1383, 2091, 2174, 2358, 2474
BseNI	ACTGG	2 cut(s)	103, 263
BseRI	GAGGAG	1 cut(s)	1542
BseSI	GKGCMC	1 cut(s)	1556
BseXI	GCAGC	4 cut(s)	271, 998, 1319, 2131
BseYI	CCCAGC	2 cut(s)	154, 422
BsgI	GTGCAG	3 cut(s)	278, 546, 2327
BshFI	GGCC	4 cut(s)	248, 1398, 1932, 2379
BshNI	GGYRCC	1 cut(s)	1046
BsiHKAI	GWGCWC	2 cut(s)	2423, 2522
BsiHKCI	CYCGRG	1 cut(s)	2315
BsiSI	CCGG	1 cut(s)	566
BslFI	GGGAC	4 cut(s)	49, 1660, 1825, 1896
BslI	CCNNNNNNNGG	8 cut(s)	240, 342, 566, 1127, 1405, 2140, 2204, 2294
BsmAI	GTCTC	4 cut(s)	111, 271, 870, 1416
BsmFI	GGGAC	4 cut(s)	49, 1660, 1825, 1896
BsnI	GGCC	4 cut(s)	248, 1398, 1932, 2379
Bso31I	GGTCTC	1 cut(s)	870
BsoBI	CYCGRG	1 cut(s)	2315
Bsp119I	TTCGAA	1 cut(s)	2073
Bsp1286I	GDGCHC	3 cut(s)	1556, 2423, 2522
Bsp1720I	GCTNAGC	2 cut(s)	1369, 2183
BspACI	CCGC	1 cut(s)	2475
BspANI	GGCC	4 cut(s)	248, 1398, 1932, 2379
BspCNI	CTCAG	9 cut(s)	311, 685, 856, 1311, 1382, 2092, 2175, 2357, 2473
BspHI	TCATGA	2 cut(s)	1837, 2232
BspLI	GGNNCC	5 cut(s)	663, 690, 1048, 1326, 2196
BspMAI	CTGCAG	1 cut(s)	1696
BspMI	ACCTGC	2 cut(s)	161, 1685
BspPI	GGATC	9 cut(s)	551, 656, 669, 767, 822, 1257, 1319, 1332, 1744
BspQI	GCTCTTC	1 cut(s)	2075
BspT104I	TTCGAA	1 cut(s)	2073
BspT107I	GGYRCC	1 cut(s)	1046
BspTI	CTTAAG	1 cut(s)	46
BspTNI	GGTCTC	1 cut(s)	870
BsrDI	GCAATG	4 cut(s)	928, 1115, 1166, 1864
BsrI	ACTGG	2 cut(s)	103, 263
BssECI	CCNNGG	2 cut(s)	872, 1606
BssSI	CACGAG	2 cut(s)	1989, 2158
BssT1I	CCWWGG	2 cut(s)	872, 1606
Bst2BI	CACGAG	2 cut(s)	1989, 2158
Bst2UI	CCWGG	4 cut(s)	486, 832, 1934, 2381
Bst4CI	ACNGT	6 cut(s)	217, 511, 621, 1091, 1277, 2216
Bst6I	CTCTTC	3 cut(s)	1945, 2075, 2220
BstAFI	CTTAAG	1 cut(s)	46
BstBAI	YACGTR	1 cut(s)	193
BstBI	TTCGAA	1 cut(s)	2073
BstC8I	GCNNGC	2 cut(s)	2149, 2254
BstEII	GGTNACC	1 cut(s)	116
BstF5I	GGATG	3 cut(s)	808, 854, 1750
BstH2I	RGCGCY	1 cut(s)	2475
BstHHI	GCGC	1 cut(s)	2474
BstMAI	GTCTC	4 cut(s)	111, 271, 870, 1416
BstMWI	GCNNNNNNNGC	4 cut(s)	37, 448, 1992, 2033
BstNI	CCWGG	4 cut(s)	486, 832, 1934, 2381
BstNSI	RCATGY	6 cut(s)	202, 386, 1114, 1661, 1729, 2429
BstPI	GGTNACC	1 cut(s)	116
BstSCI	CCNGG	5 cut(s)	484, 565, 830, 1932, 2379
BstSFI	CTRYAG	1 cut(s)	1692
BstSLI	GKGCMC	1 cut(s)	1556
BstSNI	TACGTA	1 cut(s)	193
BstV1I	GCAGC	4 cut(s)	271, 998, 1319, 2131
BstV2I	GAAGAC	3 cut(s)	1537, 2095, 2118
BstX2I	RGATCY	7 cut(s)	661, 1240, 1249, 1324, 1483, 1736, 2498
BstXI	CCANNNNNNTGG	1 cut(s)	2369
BstYI	RGATCY	7 cut(s)	661, 1240, 1249, 1324, 1483, 1736, 2498
BsuRI	GGCC	4 cut(s)	248, 1398, 1932, 2379
BtgZI	GCGATG	2 cut(s)	618, 2190
BtsCI	GGATG	3 cut(s)	808, 854, 1750
BtsI	GCAGTG	3 cut(s)	1194, 1419, 1587
BtsIMutI	CAGTG	5 cut(s)	507, 1194, 1419, 1455, 1587
BveI	ACCTGC	2 cut(s)	161, 1685
Cac8I	GCNNGC	2 cut(s)	2149, 2254
CaiI	CAGNNNCTG	1 cut(s)	269
CciI	TCATGA	2 cut(s)	1837, 2232
CfoI	GCGC	1 cut(s)	2474
Cfr13I	GGNCC	2 cut(s)	345, 2194
Csp6I	GTAC	6 cut(s)	205, 221, 1047, 1056, 1152, 1294
CviAII	CATG	9 cut(s)	199, 383, 540, 1111, 1658, 1726, 1838, 2233, 2426
CviQI	GTAC	6 cut(s)	205, 221, 1047, 1056, 1152, 1294
DrdI	GACNNNNNNGTC	1 cut(s)	1111
DseDI	GACNNNNNNGTC	1 cut(s)	1111
EaeI	YGGCCR	2 cut(s)	246, 2377
Eam1104I	CTCTTC	3 cut(s)	1945, 2075, 2220
EarI	CTCTTC	3 cut(s)	1945, 2075, 2220
Ecl136II	GAGCTC	1 cut(s)	2421
Eco105I	TACGTA	1 cut(s)	193
Eco130I	CCWWGG	2 cut(s)	872, 1606
Eco147I	AGGCCT	1 cut(s)	1398
Eco24I	GRGCYC	1 cut(s)	2423
Eco31I	GGTCTC	1 cut(s)	870
Eco47I	GGWCC	2 cut(s)	345, 2194
Eco53kI	GAGCTC	1 cut(s)	2421
Eco57I	CTGAAG	5 cut(s)	267, 706, 1100, 2130, 2523
Eco88I	CYCGRG	1 cut(s)	2315
Eco91I	GGTNACC	1 cut(s)	116
EcoICRI	GAGCTC	1 cut(s)	2421
EcoO65I	GGTNACC	1 cut(s)	116
EcoRI	GAATTC	2 cut(s)	547, 928
EcoRII	CCWGG	4 cut(s)	484, 830, 1932, 2379
EcoT14I	CCWWGG	2 cut(s)	872, 1606
EcoT22I	ATGCAT	2 cut(s)	610, 1207
EcoT38I	GRGCYC	1 cut(s)	2423
ErhI	CCWWGG	2 cut(s)	872, 1606
FaeI	CATG	9 cut(s)	202, 386, 543, 1114, 1661, 1729, 1841, 2236, 2429
FaqI	GGGAC	4 cut(s)	49, 1660, 1825, 1896
FatI	CATG	9 cut(s)	198, 382, 539, 1110, 1657, 1725, 1837, 2232, 2425
FauNDI	CATATG	1 cut(s)	1207
FbaI	TGATCA	1 cut(s)	2154
FblI	GTMKAC	1 cut(s)	1212
Fnu4HI	GCNGC	5 cut(s)	260, 1012, 1308, 2145, 2475
FokI	GGATG	3 cut(s)	815, 861, 1757
FriOI	GRGCYC	1 cut(s)	2423
Fsp4HI	GCNGC	5 cut(s)	260, 1012, 1308, 2145, 2475
FspBI	CTAG	5 cut(s)	492, 648, 777, 1107, 1895
GlaI	GCGC	1 cut(s)	2473
GluI	GCNGC	5 cut(s)	260, 1012, 1308, 2145, 2475
GsaI	CCCAGC	2 cut(s)	158, 426
GsuI	CTGGAG	1 cut(s)	1257
HaeII	RGCGCY	1 cut(s)	2475
HaeIII	GGCC	4 cut(s)	248, 1398, 1932, 2379
HapII	CCGG	1 cut(s)	566
HhaI	GCGC	1 cut(s)	2474
Hin1II	CATG	9 cut(s)	202, 386, 543, 1114, 1661, 1729, 1841, 2236, 2429
Hin6I	GCGC	1 cut(s)	2472
HinP1I	GCGC	1 cut(s)	2472
HincII	GTYRAC	1 cut(s)	1213
HindII	GTYRAC	1 cut(s)	1213
HindIII	AAGCTT	1 cut(s)	1940
HpaII	CCGG	1 cut(s)	566
HphI	GGTGA	4 cut(s)	128, 748, 1742, 2228
Hpy166II	GTNNAC	4 cut(s)	348, 1213, 2050, 2335
Hpy8I	GTNNAC	4 cut(s)	348, 1213, 2050, 2335
HpyAV	CCTTC	2 cut(s)	949, 2165
HpyCH4III	ACNGT	6 cut(s)	217, 511, 621, 1091, 1277, 2216
HpyCH4IV	ACGT	1 cut(s)	192
HpyF10VI	GCNNNNNNNGC	4 cut(s)	37, 448, 1992, 2033
HpySE526I	ACGT	1 cut(s)	192
Hsp92II	CATG	9 cut(s)	202, 386, 543, 1114, 1661, 1729, 1841, 2236, 2429
HspAI	GCGC	1 cut(s)	2472
KpnI	GGTACC	1 cut(s)	1050
Ksp22I	TGATCA	1 cut(s)	2154
LguI	GCTCTTC	1 cut(s)	2075
LmnI	GCTCC	3 cut(s)	80, 488, 653
Lsp1109I	GCAGC	4 cut(s)	271, 998, 1319, 2131
LweI	GCATC	5 cut(s)	595, 789, 1735, 2055, 2486
MaeI	CTAG	5 cut(s)	492, 648, 777, 1107, 1895
MaeII	ACGT	1 cut(s)	192
MaeIII	GTNAC	5 cut(s)	116, 511, 1495, 2029, 2162
MfeI	CAATTG	3 cut(s)	1189, 1641, 1759
MflI	RGATCY	7 cut(s)	661, 1240, 1249, 1324, 1483, 1736, 2498
MhlI	GDGCHC	3 cut(s)	1556, 2423, 2522
MlsI	TGGCCA	2 cut(s)	248, 2379
MluNI	TGGCCA	2 cut(s)	248, 2379
MlyI	GAGTC	5 cut(s)	14, 335, 679, 1974, 2411
MmeI	TCCRAC	3 cut(s)	1678, 1927, 2386
Mox20I	TGGCCA	2 cut(s)	248, 2379
Mph1103I	ATGCAT	2 cut(s)	610, 1207
MroXI	GAANNNNTTC	2 cut(s)	1619, 1887
MscI	TGGCCA	2 cut(s)	248, 2379
MseI	TTAA	6 cut(s)	47, 321, 705, 732, 819, 1320
MslI	CAYNNNNRTG	2 cut(s)	1580, 2008
Msp20I	TGGCCA	2 cut(s)	248, 2379
MspA1I	CMGCKG	2 cut(s)	137, 1373
MspCI	CTTAAG	1 cut(s)	46
MspI	CCGG	1 cut(s)	566
MspR9I	CCNGG	5 cut(s)	486, 567, 832, 1934, 2381
MunI	CAATTG	3 cut(s)	1189, 1641, 1759
MvaI	CCWGG	4 cut(s)	486, 832, 1934, 2381
MwoI	GCNNNNNNNGC	4 cut(s)	37, 448, 1992, 2033
NciI	CCSGG	1 cut(s)	567
NdeI	CATATG	1 cut(s)	1207
NlaIII	CATG	9 cut(s)	202, 386, 543, 1114, 1661, 1729, 1841, 2236, 2429
NlaIV	GGNNCC	5 cut(s)	663, 690, 1048, 1326, 2196
NmeAIII	GCCGAG	1 cut(s)	1988
NmuCI	GTSAC	2 cut(s)	116, 2029
NsiI	ATGCAT	2 cut(s)	610, 1207
NspI	RCATGY	6 cut(s)	202, 386, 1114, 1661, 1729, 2429
NspV	TTCGAA	1 cut(s)	2073
OliI	CACNNNNGTG	1 cut(s)	2008
PaeR7I	CTCGAG	1 cut(s)	2315
PagI	TCATGA	2 cut(s)	1837, 2232
PceI	AGGCCT	1 cut(s)	1398
PciI	ACATGT	2 cut(s)	382, 1657
PciSI	GCTCTTC	1 cut(s)	2075
PcsI	WCGNNNNNNNCGW	1 cut(s)	2172
PdmI	GAANNNNTTC	2 cut(s)	1619, 1887
PfeI	GAWTC	6 cut(s)	294, 792, 1166, 1565, 2127, 2311
PflMI	CCANNNNNTGG	1 cut(s)	240
PkrI	GCNGC	5 cut(s)	261, 1013, 1309, 2146, 2476
PleI	GAGTC	5 cut(s)	13, 334, 678, 1973, 2410
PpsI	GAGTC	5 cut(s)	13, 334, 678, 1973, 2410
Ppu21I	YACGTR	1 cut(s)	193
PscI	ACATGT	2 cut(s)	382, 1657
Psp124BI	GAGCTC	1 cut(s)	2423
Psp6I	CCWGG	4 cut(s)	484, 830, 1932, 2379
PspEI	GGTNACC	1 cut(s)	116
PspFI	CCCAGC	2 cut(s)	154, 422
PspGI	CCWGG	4 cut(s)	484, 830, 1932, 2379
PspN4I	GGNNCC	5 cut(s)	663, 690, 1048, 1326, 2196
PspPI	GGNCC	2 cut(s)	345, 2194
PstI	CTGCAG	1 cut(s)	1696
PstNI	CAGNNNCTG	1 cut(s)	269
PsuI	RGATCY	7 cut(s)	661, 1240, 1249, 1324, 1483, 1736, 2498
PvuII	CAGCTG	2 cut(s)	137, 1373
RsaI	GTAC	6 cut(s)	206, 222, 1048, 1057, 1153, 1295
RsaNI	GTAC	6 cut(s)	205, 221, 1047, 1056, 1152, 1294
RseI	CAYNNNNRTG	2 cut(s)	1580, 2008
SacI	GAGCTC	1 cut(s)	2423
SalI	GTCGAC	1 cut(s)	1211
SapI	GCTCTTC	1 cut(s)	2075
SaqAI	TTAA	6 cut(s)	47, 321, 705, 732, 819, 1320
SatI	GCNGC	5 cut(s)	260, 1012, 1308, 2145, 2475
Sau96I	GGNCC	2 cut(s)	345, 2194
ScaI	AGTACT	1 cut(s)	1153
SchI	GAGTC	5 cut(s)	14, 335, 679, 1974, 2411
ScrFI	CCNGG	5 cut(s)	486, 567, 832, 1934, 2381
SduI	GDGCHC	3 cut(s)	1556, 2423, 2522
SfaNI	GCATC	5 cut(s)	595, 789, 1735, 2055, 2486
SfcI	CTRYAG	1 cut(s)	1692
Sfr274I	CTCGAG	1 cut(s)	2315
SfuI	TTCGAA	1 cut(s)	2073
SinI	GGWCC	2 cut(s)	345, 2194
SlaI	CTCGAG	1 cut(s)	2315
SmiMI	CAYNNNNRTG	2 cut(s)	1580, 2008
SmlI	CTYRAG	4 cut(s)	46, 59, 581, 2315
SmoI	CTYRAG	4 cut(s)	46, 59, 581, 2315
SnaBI	TACGTA	1 cut(s)	193
SpeI	ACTAGT	1 cut(s)	647
SseBI	AGGCCT	1 cut(s)	1398
SsiI	CCGC	1 cut(s)	2475
SspMI	CTAG	5 cut(s)	492, 648, 777, 1107, 1895
SstI	GAGCTC	1 cut(s)	2423
StuI	AGGCCT	1 cut(s)	1398
StyD4I	CCNGG	5 cut(s)	484, 565, 830, 1932, 2379
StyI	CCWWGG	2 cut(s)	872, 1606
TaaI	ACNGT	6 cut(s)	217, 511, 621, 1091, 1277, 2216
TaiI	ACGT	1 cut(s)	195
TaqI	TCGA	6 cut(s)	790, 1096, 1212, 1593, 2073, 2316
TatI	WGTACW	1 cut(s)	1151
TauI	GCSGC	1 cut(s)	2477
TfiI	GAWTC	6 cut(s)	294, 792, 1166, 1565, 2127, 2311
Tru1I	TTAA	6 cut(s)	47, 321, 705, 732, 819, 1320
Tru9I	TTAA	6 cut(s)	47, 321, 705, 732, 819, 1320
TscAI	CASTG	5 cut(s)	514, 1201, 1419, 1455, 1587
TseFI	GTSAC	2 cut(s)	116, 2029
TseI	GCWGC	4 cut(s)	259, 1011, 1307, 2144
Tsp45I	GTSAC	2 cut(s)	116, 2029
TspDTI	ATGAA	9 cut(s)	423, 528, 604, 784, 995, 1449, 1557, 1826, 2445
TspGWI	ACGGA	2 cut(s)	1036, 2073
TspRI	CASTG	5 cut(s)	514, 1201, 1419, 1455, 1587
Van91I	CCANNNNNTGG	1 cut(s)	240
Vha464I	CTTAAG	1 cut(s)	46
VpaK11BI	GGWCC	2 cut(s)	345, 2194
XapI	RAATTY	4 cut(s)	23, 146, 547, 928
XceI	RCATGY	6 cut(s)	202, 386, 1114, 1661, 1729, 2429
XcmI	CCANNNNNNNNNTGG	3 cut(s)	110, 241, 474
XhoI	CTCGAG	1 cut(s)	2315
XmiI	GTMKAC	1 cut(s)	1212
XmnI	GAANNNNTTC	2 cut(s)	1619, 1887
XspI	CTAG	5 cut(s)	492, 648, 777, 1107, 1895
ZrmI	AGTACT	1 cut(s)	1153
Zsp2I	ATGCAT	2 cut(s)	610, 1207

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.