Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0006169

Physical Location & Seq

Genomic Coordinates Forward (+)

73530613 .. 73533066

Gene Structure 2454 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000021264

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2454 bp

ATGGAGTCCAAAACAAACCCAAAATTCTTCCTTTCCTTACTTTTCTTAAGCCTATGCCTCAAGTCCCATATCTGCTTTGGAGCTGACACCATCACAGCAAACCAGTCTCTCTCTGGTGACCAAACCATTGTTTCAGCTGGTGGGAAATTCGAACTGGGTTTCTTCAAACCAGGCAATACTTCAAAATACTACATAGGCATGTGGTACTACAAGACAACAGTACCGGAGCAAACCATAGTCTGGGTGGCCAATAGGGAGCAGCCAGTCTCTGATAGATTTTCTTCAGAGTTGAGAATCTCAGATGGTAATTTGGTGCTGTTTAATGAGTCCAAAACCCCAATTTGGTCCACAGATGTCAGTTCAAGTTCAGCCTCTTCTATACATGTAGTTCTTTTAGATAATGGAAACCTTGTGCTAAGAGCTGGGTCTAATTCATCTTTGCCTTTATGGCAAAGTTTTGATTATCCAGCTCATACTTGGCTACCAGAAGCTAGAGTTGGGTTCAACACTGTTACAAACCGAACCCAAGTCCTGACTTCATGGAAGAATTTTGAGGATCCTGCACCGGGTATTTACTCTCTCCTGCGTGACCCGAATGGAAGTGATGCATATTTCTCACTGTGGAATATGTCTAAGCAGTATTGGACTAGTGGAGCTTGGGATGCAAAGAGTCGTATTTTCAGTATGGTTCCTGAGATGCATCTAACCTATATCTACAACTTCAGCTATGTTAAAACCAAAAATGAGAGCTACTTCACCTATTCTGTTTATAATTCTAGCATCACATCTCGATTCATAATGGATGTATCGGGTGAGGTTAAGCAATTTTCCTGGTTGCAACCTCAGGGATGGAACTTGATTTGGTCTCAGCCGAGGAAACTATGTCAGGTTTATTCCTATTGTGGGGCATTTGGCAGTTGCAATGAGAAGTCCTTGCCCTTCTGTAATTGTTTATATGGTTTTGAGCCGAAATTGAAGGTGGATTGGAATTCGAAGGTTTATTCTGGTGGCTGCAAAAGACGGACTGTGCTGAATTTTGCTAATGCTACTACCAATAATGGGAAGCAAGACCGGTTTCTAGAAATGCCTCACATGTCTTTGCCTGACAGTGAAGTGTCTGTAGAGGCTGGGAGTAATGCGCAATGTGAATCAATCTGCTTAAGTAACCGCTCTTGCACTGCTTATGCTTATGATAGCAATGGATGTTCAATATGGATTGGAGATCTCTTGAATCAGCAACAACTCACGGCAGATGACGGTAATGGAAAAACTTTGTACCTCAGACTTGCAGCTTCCGAGTTTAAGGATCCGAAAAGTAAAAAGGGATTGATTATCAGTGTTGCAGTAGGCTCAGCTGTTGGGATAGCAGTTCTCTTAGGCCTTATTGTGTTTAGAGTGTTGAGAAACAGATATAGAGTGATTGGAAAATCAATGGGGGGTTCATTGGTGGCATTTGAGTATAGAGATCTTCAAGACGTAACAAAGAACTTCTCGGAGAAATTGGGGGGAGGAGGTTTTGGTTCCGTCTTCAAGGGCACAATGCCTGATTCATCAGTCATAGCAGTGAAGAAGCTCCAAAGTGTTAGCCAAGGAGAGAAGCAATTCAGAACAGAAGTCAGCACAATTGGGACAATCCAACATGTCAATCTTGTTCGGCTTCGTGGGTTCTGCTCTGAAGGTGCCAAGAGGTTGTTGGTCTATGATTACATGCCTAATGGATCTCTGGATGCTCACCTTTTCAATCACCCAAGTCATCTTTTGGAGTGGAATACAAGATACCAAATTGCTCTGGGTACAGCAAGAGGGTTGGCTTATCTTCATGAGAAATGTAGAGACTGTATCATACATTGTGACATAAAGCCAGAGAACATTCTCCTAGATACCGAACTTTGTCCCAAAGTAGCAGACTTTGGCTTGGCAAAGCTTGTTGGAAGAGAATTCAGCCGAGTCCTGACCACAATGAGAGGCACGCCAGGCTATCTTGCACCAGAGTGGATTTCAGGAGTGGCAGTCACAGCAAAAGCTGATGTTTACAGCTACGGAATGATGCTCTTCGAAATTGTATCAGGAAGACGAAACACTGAGCCATCTGAAGACGGCAAAGTTAGATTCTTCCCAACTCGGGCTGCTGGCGTGATCACGAGTAACGAAGAAGCCGATGTGCTGAGCCTATTGGACCCGAAATTAGGGAGAAACGGTGATGTGGAAGAGATCATGAGGGTTCTTAGAGTTGCTTGCTGGTGTGTCCAAGATGATGAGGCTCATAGACCATCAATGGGCCAAGTGGTGCAGATTCTCGAGGGGGTTTTGAATGTGAACTTGCCCTCAGTTCCAAGATCTCTCCAACTCTTTGGAGATAGCCAGCACATAGTTTTCTTCACCGAGTCATCTGCAAGCCAGAGCTCACATGCACCAACCAATAATACATCAACAGCTTCATCGATCTCAGACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

B_lectin PAN_2 Pkinase S_locus_glycop

Protein Analysis

818

Amino Acids

90.81

Weight (kDa)

7.97

Isoelectric Point (pI)

38.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	78 - 182	1.6e-33	D-mannose binding lectin
S_locus_glycop	PF00954	215 - 324	8.7e-29	S-locus glycoprotein domain
PAN_2	PF08276	355 - 411	2e-14	PAN-like domain
PK_Tyr_Ser-Thr	PF07714	497 - 765	3e-41	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	498 - 764	1.2e-46	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0006169)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G19130
fragaria_vesca	FvH4_6g44310
malus_domestica	MD09G1098900.v1.1 MD17G1087200.v1.1
prunus_persica	Prupe.3G227300_v2.0.a1 Prupe.3G227500_v2.0.a1
pyrus_communis	pycom09g02260 pycom17g08440
rosa_chinensis	RchiOBHm_Chr2g0161171 RchiOBHm_Chr2g0161191
rosa_laevigata	RLG00000021264 RLG00000021266 RLG00000029382
rosa_multiflora	Rmu_sc0006420.1_g000040 Rmu_sc0006420.1_g000045
rosa_roxburghii	Rroxscaffold_2G00089040 Rroxscaffold_2G00089060
rosa_rugosa	Rorug02G0486800 Rorug02G0486900
rosa_samantha	Rh2AG553500 Rh2BG567200 Rh2CG537300 Rh2CG537500 Rh2DG576500 Rh2DG576600
rosa_wichuraiana	Rw2G045780 Rw2G045800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	771
Acc16I	TGCGCA	1 cut(s)	1140
AccB1I	GGYRCC	1 cut(s)	1679
AccB7I	CCANNNNNTGG	1 cut(s)	240
AccBSI	CCGCTC	1 cut(s)	1170
AciI	CCGC	1 cut(s)	1168
AclWI	GGATC	5 cut(s)	551, 564, 1301, 1314, 1726
AcoI	YGGCCR	1 cut(s)	246
AcsI	RAATTY	6 cut(s)	23, 146, 547, 988, 1033, 1937
AcuI	CTGAAG	4 cut(s)	267, 706, 1695, 2112
AfaI	GTAC	4 cut(s)	206, 222, 1277, 1795
AfiI	CCNNNNNNNGG	8 cut(s)	240, 342, 566, 903, 1059, 2122, 2186, 2276
AflII	CTTAAG	2 cut(s)	46, 1159
AflIII	ACRYGT	3 cut(s)	382, 1092, 1639
AgeI	ACCGGT	1 cut(s)	1071
AhlI	ACTAGT	1 cut(s)	647
AjnI	CCWGG	3 cut(s)	169, 830, 1972
AjuI	GAANNNNNNNTTGG	2 cut(s)	480, 512
AleI	CACNNNNGTG	1 cut(s)	1990
Alw21I	GWGCWC	1 cut(s)	2405
Alw26I	GTCTC	4 cut(s)	111, 271, 870, 1827
AlwI	GGATC	5 cut(s)	551, 564, 1301, 1314, 1726
AlwNI	CAGNNNCTG	1 cut(s)	269
Ama87I	CYCGRG	2 cut(s)	2121, 2297
AoxI	GGCC	3 cut(s)	246, 1378, 2278
ApeKI	GCWGC	4 cut(s)	259, 1011, 1289, 2126
ApoI	RAATTY	6 cut(s)	23, 146, 547, 988, 1033, 1937
AsiGI	ACCGGT	1 cut(s)	1071
Asp700I	GAANNNNTTC	1 cut(s)	1601
AspLEI	GCGC	1 cut(s)	1141
AspS9I	GGNCC	3 cut(s)	345, 2176, 2278
AsuC2I	CCSGG	1 cut(s)	567
AsuHPI	GGTGA	7 cut(s)	128, 748, 824, 1724, 1736, 2210, 2371
AsuII	TTCGAA	3 cut(s)	150, 992, 2055
AvaI	CYCGRG	2 cut(s)	2121, 2297
AvaII	GGWCC	2 cut(s)	345, 2176
AxyI	CCTNAGG	1 cut(s)	843
BaeGI	GKGCMC	1 cut(s)	1538
BaeI	ACNNNNGTAYC	2 cut(s)	204, 237
BalI	TGGCCA	1 cut(s)	248
BamHI	GGATCC	2 cut(s)	556, 1306
BanI	GGYRCC	1 cut(s)	1679
BanII	GRGCYC	1 cut(s)	2405
BauI	CACGAG	1 cut(s)	2140
BbsI	GAAGAC	3 cut(s)	1519, 2077, 2100
Bbv12I	GWGCWC	1 cut(s)	2405
BbvI	GCAGC	4 cut(s)	271, 998, 1301, 2113
BccI	CCATC	5 cut(s)	98, 296, 843, 2095, 2278
BceAI	ACGGC	2 cut(s)	1263, 2113
BcgI	CGANNNNNNTGC	2 cut(s)	2372, 2406
BciT130I	CCWGG	3 cut(s)	171, 832, 1974
BclI	TGATCA	1 cut(s)	2136
BcnI	CCSGG	1 cut(s)	567
BcoDI	GTCTC	4 cut(s)	111, 271, 870, 1827
BcuI	ACTAGT	1 cut(s)	647
BfaI	CTAG	5 cut(s)	492, 648, 777, 1079, 1877
BfmI	CTRYAG	1 cut(s)	1119
BfrI	CTTAAG	2 cut(s)	46, 1159
BglI	GCCNNNNNGGC	1 cut(s)	448
BglII	AGATCT	3 cut(s)	1222, 1465, 2336
BisI	GCNGC	4 cut(s)	260, 1012, 1290, 2127
BlpI	GCTNAGC	2 cut(s)	1351, 2165
BlsI	GCNGC	4 cut(s)	261, 1013, 1291, 2128
Bme1390I	CCNGG	4 cut(s)	171, 567, 832, 1974
Bme18I	GGWCC	2 cut(s)	345, 2176
BmeT110I	CYCGRG	2 cut(s)	2121, 2297
BmgT120I	GGNCC	3 cut(s)	345, 2176, 2278
BmiI	GGNNCC	6 cut(s)	558, 690, 1308, 1522, 1681, 2178
BmrFI	CCNGG	4 cut(s)	171, 567, 832, 1974
BmrI	ACTGGG	1 cut(s)	164
BmsI	GCATC	7 cut(s)	595, 652, 687, 709, 789, 1717, 2037
BmuI	ACTGGG	1 cut(s)	164
BpiI	GAAGAC	3 cut(s)	1519, 2077, 2100
Bpu1102I	GCTNAGC	2 cut(s)	1351, 2165
Bpu14I	TTCGAA	3 cut(s)	150, 992, 2055
BpuEI	CTTGAG	1 cut(s)	44
BpuMI	CCSGG	1 cut(s)	567
Bsa29I	ATCGAT	1 cut(s)	2441
BsaI	GGTCTC	1 cut(s)	870
BsaJI	CCNNGG	2 cut(s)	872, 1588
BsaWI	WCCGGW	2 cut(s)	223, 1071
BsaXI	ACNNNNNCTCC	4 cut(s)	1995, 2025, 2182, 2212
Bsc4I	CCNNNNNNNGG	8 cut(s)	240, 342, 566, 903, 1059, 2122, 2186, 2276
Bse118I	RCCGGY	1 cut(s)	1071
Bse1I	ACTGG	3 cut(s)	103, 159, 263
Bse21I	CCTNAGG	1 cut(s)	843
Bse3DI	GCAATG	3 cut(s)	928, 1148, 1204
BseBI	CCWGG	3 cut(s)	171, 832, 1974
BseCI	ATCGAT	1 cut(s)	2441
BseDI	CCNNGG	2 cut(s)	872, 1588
BseGI	GGATG	5 cut(s)	667, 808, 854, 1208, 1732
BseLI	CCNNNNNNNGG	8 cut(s)	240, 342, 566, 903, 1059, 2122, 2186, 2276
BseMI	GCAATG	3 cut(s)	928, 1148, 1204
BseMII	CTCAG	9 cut(s)	312, 684, 857, 881, 1294, 1365, 2073, 2156, 2340
BseNI	ACTGG	3 cut(s)	103, 159, 263
BseRI	GAGGAG	1 cut(s)	1524
BseSI	GKGCMC	1 cut(s)	1538
BseXI	GCAGC	4 cut(s)	271, 998, 1301, 2113
BseYI	CCCAGC	2 cut(s)	422, 1127
BsgI	GTGCAG	2 cut(s)	546, 2309
BshFI	GGCC	3 cut(s)	248, 1380, 2280
BshNI	GGYRCC	1 cut(s)	1679
BshTI	ACCGGT	1 cut(s)	1071
BshVI	ATCGAT	1 cut(s)	2441
BsiHKAI	GWGCWC	1 cut(s)	2405
BsiHKCI	CYCGRG	2 cut(s)	2121, 2297
BsiSI	CCGG	3 cut(s)	224, 566, 1072
BslFI	GGGAC	3 cut(s)	49, 1642, 1878
BslI	CCNNNNNNNGG	8 cut(s)	240, 342, 566, 903, 1059, 2122, 2186, 2276
BsmAI	GTCTC	4 cut(s)	111, 271, 870, 1827
BsmFI	GGGAC	3 cut(s)	49, 1642, 1878
BsnI	GGCC	3 cut(s)	248, 1380, 2280
Bso31I	GGTCTC	1 cut(s)	870
BsoBI	CYCGRG	2 cut(s)	2121, 2297
Bsp119I	TTCGAA	3 cut(s)	150, 992, 2055
Bsp1286I	GDGCHC	2 cut(s)	1538, 2405
Bsp143I	GATC	9 cut(s)	556, 1222, 1306, 1465, 1718, 2136, 2211, 2336, 2442
Bsp1720I	GCTNAGC	2 cut(s)	1351, 2165
BspACI	CCGC	1 cut(s)	1168
BspANI	GGCC	3 cut(s)	248, 1380, 2280
BspCNI	CTCAG	9 cut(s)	311, 685, 856, 880, 1293, 1364, 2074, 2157, 2339
BspDI	ATCGAT	1 cut(s)	2441
BspHI	TCATGA	2 cut(s)	1819, 2214
BspLI	GGNNCC	6 cut(s)	558, 690, 1308, 1522, 1681, 2178
BspPI	GGATC	5 cut(s)	551, 564, 1301, 1314, 1726
BspQI	GCTCTTC	1 cut(s)	2057
BspT104I	TTCGAA	3 cut(s)	150, 992, 2055
BspT107I	GGYRCC	1 cut(s)	1679
BspTI	CTTAAG	2 cut(s)	46, 1159
BspTNI	GGTCTC	1 cut(s)	870
BsrBI	CCGCTC	1 cut(s)	1170
BsrDI	GCAATG	3 cut(s)	928, 1148, 1204
BsrFI	RCCGGY	1 cut(s)	1071
BsrI	ACTGG	3 cut(s)	103, 159, 263
BssAI	RCCGGY	1 cut(s)	1071
BssECI	CCNNGG	2 cut(s)	872, 1588
BssMI	GATC	9 cut(s)	556, 1222, 1306, 1465, 1718, 2136, 2211, 2336, 2442
BssSI	CACGAG	1 cut(s)	2140
BssT1I	CCWWGG	1 cut(s)	1588
Bst2BI	CACGAG	1 cut(s)	2140
Bst2UI	CCWGG	3 cut(s)	171, 832, 1974
Bst4CI	ACNGT	8 cut(s)	220, 511, 621, 1027, 1109, 1259, 1838, 2198
Bst6I	CTCTTC	4 cut(s)	379, 1927, 2057, 2202
BstAFI	CTTAAG	2 cut(s)	46, 1159
BstBI	TTCGAA	3 cut(s)	150, 992, 2055
BstC8I	GCNNGC	5 cut(s)	1970, 2131, 2236, 2363, 2395
BstEII	GGTNACC	1 cut(s)	116
BstF5I	GGATG	5 cut(s)	667, 808, 854, 1208, 1732
BstHHI	GCGC	1 cut(s)	1141
BstKTI	GATC	9 cut(s)	559, 1225, 1309, 1468, 1721, 2139, 2214, 2339, 2445
BstMAI	GTCTC	4 cut(s)	111, 271, 870, 1827
BstMBI	GATC	9 cut(s)	556, 1222, 1306, 1465, 1718, 2136, 2211, 2336, 2442
BstMWI	GCNNNNNNNGC	4 cut(s)	448, 662, 1974, 2015
BstNI	CCWGG	3 cut(s)	171, 832, 1974
BstNSI	RCATGY	6 cut(s)	202, 386, 1096, 1643, 1711, 2411
BstPI	GGTNACC	1 cut(s)	116
BstSCI	CCNGG	4 cut(s)	169, 565, 830, 1972
BstSFI	CTRYAG	1 cut(s)	1119
BstSLI	GKGCMC	1 cut(s)	1538
BstV1I	GCAGC	4 cut(s)	271, 998, 1301, 2113
BstV2I	GAAGAC	3 cut(s)	1519, 2077, 2100
BstX2I	RGATCY	6 cut(s)	556, 1222, 1306, 1465, 1718, 2336
BstXI	CCANNNNNNTGG	1 cut(s)	2351
BstYI	RGATCY	6 cut(s)	556, 1222, 1306, 1465, 1718, 2336
Bsu15I	ATCGAT	1 cut(s)	2441
Bsu36I	CCTNAGG	1 cut(s)	843
BsuRI	GGCC	3 cut(s)	248, 1380, 2280
BsuTUI	ATCGAT	1 cut(s)	2441
BtsCI	GGATG	5 cut(s)	667, 808, 854, 1208, 1732
BtsI	GCAGTG	2 cut(s)	1176, 1569
BtsIMutI	CAGTG	7 cut(s)	507, 617, 1114, 1176, 1342, 1569, 2079
Cac8I	GCNNGC	5 cut(s)	1970, 2131, 2236, 2363, 2395
CaiI	CAGNNNCTG	1 cut(s)	269
CciI	TCATGA	2 cut(s)	1819, 2214
CfoI	GCGC	1 cut(s)	1141
Cfr10I	RCCGGY	1 cut(s)	1071
Cfr13I	GGNCC	3 cut(s)	345, 2176, 2278
ClaI	ATCGAT	1 cut(s)	2441
Csp6I	GTAC	4 cut(s)	205, 221, 1276, 1794
CspAI	ACCGGT	1 cut(s)	1071
CviAII	CATG	9 cut(s)	199, 383, 540, 1093, 1640, 1708, 1820, 2215, 2408
CviQI	GTAC	4 cut(s)	205, 221, 1276, 1794
DpnI	GATC	9 cut(s)	558, 1224, 1308, 1467, 1720, 2138, 2213, 2338, 2444
DpnII	GATC	9 cut(s)	556, 1222, 1306, 1465, 1718, 2136, 2211, 2336, 2442
EaeI	YGGCCR	1 cut(s)	246
Eam1104I	CTCTTC	4 cut(s)	379, 1927, 2057, 2202
EarI	CTCTTC	4 cut(s)	379, 1927, 2057, 2202
Ecl136II	GAGCTC	1 cut(s)	2403
Eco130I	CCWWGG	1 cut(s)	1588
Eco147I	AGGCCT	1 cut(s)	1380
Eco24I	GRGCYC	1 cut(s)	2405
Eco31I	GGTCTC	1 cut(s)	870
Eco47I	GGWCC	2 cut(s)	345, 2176
Eco53kI	GAGCTC	1 cut(s)	2403
Eco57I	CTGAAG	4 cut(s)	267, 706, 1695, 2112
Eco81I	CCTNAGG	1 cut(s)	843
Eco88I	CYCGRG	2 cut(s)	2121, 2297
Eco91I	GGTNACC	1 cut(s)	116
EcoICRI	GAGCTC	1 cut(s)	2403
EcoO65I	GGTNACC	1 cut(s)	116
EcoRI	GAATTC	2 cut(s)	988, 1937
EcoRII	CCWGG	3 cut(s)	169, 830, 1972
EcoT14I	CCWWGG	1 cut(s)	1588
EcoT22I	ATGCAT	2 cut(s)	610, 702
EcoT38I	GRGCYC	1 cut(s)	2405
ErhI	CCWWGG	1 cut(s)	1588
FaeI	CATG	9 cut(s)	202, 386, 543, 1096, 1643, 1711, 1823, 2218, 2411
FaqI	GGGAC	3 cut(s)	49, 1642, 1878
FatI	CATG	9 cut(s)	198, 382, 539, 1092, 1639, 1707, 1819, 2214, 2407
FbaI	TGATCA	1 cut(s)	2136
Fnu4HI	GCNGC	4 cut(s)	260, 1012, 1290, 2127
FokI	GGATG	5 cut(s)	674, 815, 861, 1215, 1739
FriOI	GRGCYC	1 cut(s)	2405
Fsp4HI	GCNGC	4 cut(s)	260, 1012, 1290, 2127
FspBI	CTAG	5 cut(s)	492, 648, 777, 1079, 1877
FspI	TGCGCA	1 cut(s)	1140
GlaI	GCGC	1 cut(s)	1140
GluI	GCNGC	4 cut(s)	260, 1012, 1290, 2127
GsaI	CCCAGC	2 cut(s)	426, 1131
HaeIII	GGCC	3 cut(s)	248, 1380, 2280
HapII	CCGG	3 cut(s)	224, 566, 1072
HhaI	GCGC	1 cut(s)	1141
Hin1II	CATG	9 cut(s)	202, 386, 543, 1096, 1643, 1711, 1823, 2218, 2411
Hin6I	GCGC	1 cut(s)	1139
HinP1I	GCGC	1 cut(s)	1139
HindIII	AAGCTT	1 cut(s)	1922
HpaII	CCGG	3 cut(s)	224, 566, 1072
HphI	GGTGA	7 cut(s)	128, 748, 824, 1724, 1736, 2210, 2371
Hpy166II	GTNNAC	3 cut(s)	348, 2032, 2317
Hpy8I	GTNNAC	3 cut(s)	348, 2032, 2317
HpyAV	CCTTC	4 cut(s)	949, 970, 988, 1670
HpyCH4III	ACNGT	8 cut(s)	220, 511, 621, 1027, 1109, 1259, 1838, 2198
HpyCH4IV	ACGT	1 cut(s)	1476
HpyF10VI	GCNNNNNNNGC	4 cut(s)	448, 662, 1974, 2015
HpySE526I	ACGT	1 cut(s)	1476
Hsp92II	CATG	9 cut(s)	202, 386, 543, 1096, 1643, 1711, 1823, 2218, 2411
HspAI	GCGC	1 cut(s)	1139
Ksp22I	TGATCA	1 cut(s)	2136
Kzo9I	GATC	9 cut(s)	556, 1222, 1306, 1465, 1718, 2136, 2211, 2336, 2442
LguI	GCTCTTC	1 cut(s)	2057
LmnI	GCTCC	5 cut(s)	80, 226, 256, 653, 1578
Lsp1109I	GCAGC	4 cut(s)	271, 998, 1301, 2113
LweI	GCATC	7 cut(s)	595, 652, 687, 709, 789, 1717, 2037
MaeI	CTAG	5 cut(s)	492, 648, 777, 1079, 1877
MaeII	ACGT	1 cut(s)	1476
MaeIII	GTNAC	8 cut(s)	116, 511, 587, 1163, 1477, 1850, 2011, 2144
MalI	GATC	9 cut(s)	558, 1224, 1308, 1467, 1720, 2138, 2213, 2338, 2444
MbiI	CCGCTC	1 cut(s)	1170
MboI	GATC	9 cut(s)	556, 1222, 1306, 1465, 1718, 2136, 2211, 2336, 2442
MfeI	CAATTG	1 cut(s)	1623
MflI	RGATCY	6 cut(s)	556, 1222, 1306, 1465, 1718, 2336
MhlI	GDGCHC	2 cut(s)	1538, 2405
MlsI	TGGCCA	1 cut(s)	248
MluNI	TGGCCA	1 cut(s)	248
MlyI	GAGTC	5 cut(s)	14, 335, 679, 1956, 2393
MmeI	TCCRAC	3 cut(s)	1660, 1909, 2368
Mox20I	TGGCCA	1 cut(s)	248
Mph1103I	ATGCAT	2 cut(s)	610, 702
MroXI	GAANNNNTTC	1 cut(s)	1601
MscI	TGGCCA	1 cut(s)	248
MseI	TTAA	6 cut(s)	47, 321, 732, 819, 1160, 1302
MslI	CAYNNNNRTG	3 cut(s)	197, 1562, 1990
Msp20I	TGGCCA	1 cut(s)	248
MspA1I	CMGCKG	2 cut(s)	137, 1355
MspCI	CTTAAG	2 cut(s)	46, 1159
MspI	CCGG	3 cut(s)	224, 566, 1072
MspR9I	CCNGG	4 cut(s)	171, 567, 832, 1974
MunI	CAATTG	1 cut(s)	1623
MvaI	CCWGG	3 cut(s)	171, 832, 1974
MwoI	GCNNNNNNNGC	4 cut(s)	448, 662, 1974, 2015
NciI	CCSGG	1 cut(s)	567
NdeII	GATC	9 cut(s)	556, 1222, 1306, 1465, 1718, 2136, 2211, 2336, 2442
NlaIII	CATG	9 cut(s)	202, 386, 543, 1096, 1643, 1711, 1823, 2218, 2411
NlaIV	GGNNCC	6 cut(s)	558, 690, 1308, 1522, 1681, 2178
NmeAIII	GCCGAG	2 cut(s)	897, 1970
NmuCI	GTSAC	4 cut(s)	116, 587, 1850, 2011
NsbI	TGCGCA	1 cut(s)	1140
NsiI	ATGCAT	2 cut(s)	610, 702
NspI	RCATGY	6 cut(s)	202, 386, 1096, 1643, 1711, 2411
NspV	TTCGAA	3 cut(s)	150, 992, 2055
OliI	CACNNNNGTG	1 cut(s)	1990
PaeR7I	CTCGAG	1 cut(s)	2297
PagI	TCATGA	2 cut(s)	1819, 2214
PceI	AGGCCT	1 cut(s)	1380
PciI	ACATGT	3 cut(s)	382, 1092, 1639
PciSI	GCTCTTC	1 cut(s)	2057
PcsI	WCGNNNNNNNCGW	1 cut(s)	2154
PdmI	GAANNNNTTC	1 cut(s)	1601
PfeI	GAWTC	7 cut(s)	294, 792, 1148, 1231, 1547, 2109, 2293
PflMI	CCANNNNNTGG	1 cut(s)	240
PinAI	ACCGGT	1 cut(s)	1071
PkrI	GCNGC	4 cut(s)	261, 1013, 1291, 2128
PleI	GAGTC	5 cut(s)	13, 334, 678, 1955, 2392
PpsI	GAGTC	5 cut(s)	13, 334, 678, 1955, 2392
PscI	ACATGT	3 cut(s)	382, 1092, 1639
PsiI	TTATAA	1 cut(s)	771
Psp124BI	GAGCTC	1 cut(s)	2405
Psp6I	CCWGG	3 cut(s)	169, 830, 1972
PspEI	GGTNACC	1 cut(s)	116
PspFI	CCCAGC	2 cut(s)	422, 1127
PspGI	CCWGG	3 cut(s)	169, 830, 1972
PspN4I	GGNNCC	6 cut(s)	558, 690, 1308, 1522, 1681, 2178
PspPI	GGNCC	3 cut(s)	345, 2176, 2278
PstNI	CAGNNNCTG	1 cut(s)	269
PsuI	RGATCY	6 cut(s)	556, 1222, 1306, 1465, 1718, 2336
PvuII	CAGCTG	2 cut(s)	137, 1355
RsaI	GTAC	4 cut(s)	206, 222, 1277, 1795
RsaNI	GTAC	4 cut(s)	205, 221, 1276, 1794
RseI	CAYNNNNRTG	3 cut(s)	197, 1562, 1990
SacI	GAGCTC	1 cut(s)	2405
SapI	GCTCTTC	1 cut(s)	2057
SaqAI	TTAA	6 cut(s)	47, 321, 732, 819, 1160, 1302
SatI	GCNGC	4 cut(s)	260, 1012, 1290, 2127
Sau3AI	GATC	9 cut(s)	556, 1222, 1306, 1465, 1718, 2136, 2211, 2336, 2442
Sau96I	GGNCC	3 cut(s)	345, 2176, 2278
SchI	GAGTC	5 cut(s)	14, 335, 679, 1956, 2393
ScrFI	CCNGG	4 cut(s)	171, 567, 832, 1974
SduI	GDGCHC	2 cut(s)	1538, 2405
SfaNI	GCATC	7 cut(s)	595, 652, 687, 709, 789, 1717, 2037
SfcI	CTRYAG	1 cut(s)	1119
Sfr274I	CTCGAG	1 cut(s)	2297
SfuI	TTCGAA	3 cut(s)	150, 992, 2055
SinI	GGWCC	2 cut(s)	345, 2176
SlaI	CTCGAG	1 cut(s)	2297
SmiMI	CAYNNNNRTG	3 cut(s)	197, 1562, 1990
SmlI	CTYRAG	4 cut(s)	46, 59, 1159, 2297
SmoI	CTYRAG	4 cut(s)	46, 59, 1159, 2297
SpeI	ACTAGT	1 cut(s)	647
SseBI	AGGCCT	1 cut(s)	1380
SsiI	CCGC	1 cut(s)	1168
SspMI	CTAG	5 cut(s)	492, 648, 777, 1079, 1877
SstI	GAGCTC	1 cut(s)	2405
StuI	AGGCCT	1 cut(s)	1380
StyD4I	CCNGG	4 cut(s)	169, 565, 830, 1972
StyI	CCWWGG	1 cut(s)	1588
TaaI	ACNGT	8 cut(s)	220, 511, 621, 1027, 1109, 1259, 1838, 2198
TaiI	ACGT	1 cut(s)	1479
TaqI	TCGA	6 cut(s)	150, 790, 992, 2055, 2298, 2441
TfiI	GAWTC	7 cut(s)	294, 792, 1148, 1231, 1547, 2109, 2293
Tru1I	TTAA	6 cut(s)	47, 321, 732, 819, 1160, 1302
Tru9I	TTAA	6 cut(s)	47, 321, 732, 819, 1160, 1302
TscAI	CASTG	7 cut(s)	514, 624, 1114, 1183, 1342, 1569, 2086
TseFI	GTSAC	4 cut(s)	116, 587, 1850, 2011
TseI	GCWGC	4 cut(s)	259, 1011, 1289, 2126
Tsp45I	GTSAC	4 cut(s)	116, 587, 1850, 2011
TspDTI	ATGAA	7 cut(s)	423, 528, 784, 1431, 1539, 1808, 2427
TspGWI	ACGGA	3 cut(s)	1036, 1513, 2055
TspRI	CASTG	7 cut(s)	514, 624, 1114, 1183, 1342, 1569, 2086
Van91I	CCANNNNNTGG	1 cut(s)	240
Vha464I	CTTAAG	2 cut(s)	46, 1159
VpaK11BI	GGWCC	2 cut(s)	345, 2176
XapI	RAATTY	6 cut(s)	23, 146, 547, 988, 1033, 1937
XbaI	TCTAGA	1 cut(s)	1078
XceI	RCATGY	6 cut(s)	202, 386, 1096, 1643, 1711, 2411
XcmI	CCANNNNNNNNNTGG	5 cut(s)	74, 110, 241, 474, 1690
XhoI	CTCGAG	1 cut(s)	2297
XmnI	GAANNNNTTC	1 cut(s)	1601
XspI	CTAG	5 cut(s)	492, 648, 777, 1079, 1877
Zsp2I	ATGCAT	2 cut(s)	610, 702

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.