Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0006169

Physical Location & Seq

Genomic Coordinates Forward (+)

72889957 .. 72894261

Gene Structure 4305 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G045780.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2469 bp

ATGACAGAGACGGCCATGGAGTCCAAAACAAACCCAAAATTCTTGCTAGCCTTGCTTTTCTTAAGCCTATGCCTCAAGTCCCATATCTGCTTTGGAGCTGACACCATCACAGCAAACCAGTCTCTCTCTGGTGACCAAACCATTGTTTCAGCTCGTGGGAAATTTGAACTGGGTTTCTTCAAACCAGGCAATACTTCAAAATACTACATAGGCATGTGGTACTACAAGACAACAGTACCAGAGCAAACCATAGTCTGGGTGGCCAATAGGGAGCAGCCAGTCTCTGATAGATTTTCTTCAGAGTTGAGAATCTCAGATGGTAATTTGGTGCTGTTTAATGAGTCCAAAACCCCAATTTGGTCCACAGATGTCAGTTCAAGTTCAGCCTCTTCTATACATGTAGTTCTTTTAGATAATGGAAACCTTGTGCTAAGAGCTGGGTCTAATTCATCTTTGCCTTTATGGCAAAGTTTTGATTATCCAGCTCATACTTGGCTACCAGAAGCTAGAGTTGGGTTCAACACTGTTACAAACCGAACCCAAGTCCTGACTTCATGGAAGAATTTTGAGGATCCTGCACCGGGTATTTACTCTCTAGTGCTTGACTCGAATGGAAGTGATACATATTTCTCACTGTGGAATATGTCTAAGCAGTATTGGACTAGTGGAGCTTGGGATGCAAAGAGTCGTATTTTCAGTATGGTTCCTGAGATGCATCTAACCTATATCTACAACTTCAGCTATGTTAAAACCAAAAATGAGAGCTACTTCACCTATTCTCTTTATGATTCTAGCATCACATCTCGATTCATTATGGATGTATCGGGTCAGGTTAAGCAATTTTCCTGGTTGCAAGATCAGGGATGGAACTTGATTTGGTCTCAGCCGAGGAAACTATGTCAGGTTTATTCCTATTGTGGGGCATTTGGCAGTTGCAATGAGACGTCCTTGCCCTTCTGTAATTGTTTATATGGTTTTGAGCCGAAATTGAAGGTGGATTGGAATTCGCAGGTTTATTCTGGTGGCTGCAAAAGACGGACTGTGCTGAATTTTGCTAATGCTACTACCAACAATGGGAAGCAAGACCGGTTTTTAGAAATGCCTAGCATGTCTTTGCCTGACAGTGAAGTGTCTGTAGAGGCTGGGAGGACTGCGCAATGTGAATCAATCTGCTTAAGTAACCGCTCTTGCACTGCTTATGCTTATGATAGCAATGGATGTTCAATATGGATTGGAGATCTCTTGAATCTGCAACAACTCACAGCAGATGACGGTGATGGAAAAACTTTGTACCTCAGACTTGCAGCTTCCGAGTTTAAGGATCCAAAAAGTAAAAAGGGATTGATTATCGGTGTTGCAGTAGGCTCAGCTGTTGGGATAGCAGTTCTTTTAGGCCTTATTGTGTTTAGAGTGTTGAGAAACAGATATAGAGTGATTGGAAAATCAGTGGAGGGTTCATTGGTGGCATTTGAGTATAGAGATCTTCAAGAAGTAACAAAGAACTTCTCGGAGAAATTGGGGGGAGGAGGTTTTGGTTCTGTCTTCAAGGGCACAATGCCTGATTCATCAGTCATAGCAGTGAAGAAGCTCGGAAGTGTTAGCCAAGGAGAGAAGCAATTCAGAACAGAAGTCAGCACAATTGGGACAATCCAACATGTCAATCTTGTTCGGTTTCGTGGGTTCTGCTCTGAAGGTGCCAAGAGGTTGTTGGTCTATGATTACATGCCTAATGGATCTCTGGATGCTCACCTTTTCAATCGTCCAAGCGATGTTTTGGAGTGGAATACAAGATACCAAATTGCTCGGGGTACAGCAAGAGGGTTGGCTTATCTTCATGAGAAATGTAGAGATTGTATCATACATTGTGACATAAAGCCAGAGAACATTCTCCTAGATAACGAACTTTGTCCCAAAGTAGCAGACTTTGGCCTGGCAAAGCTTGTTGGAAGAGAATTCAGCCGAGTCCTGACCACAATGAGAGGCACGCCAGGCTATCTTGCACCAGAGTGGATTTCAGGAGTGGCAGTCACAGCAAAAGCTGATGTTTACAGCTACGGAATGATGCTCTTCGAAATTGTATCAGGAAGACGAAACTCTGAGCCATCTGAAGACGGCAAACTTAGATTCTTCCCAACTCGGGCTGCTTTCGTGATCACGAGTAACGAAGAAGGCGATGTGCTGAGCCTATTGGACCCGAAATTAGGGAGAAATGGTGATGTGGAAGAGATCATGAGGGTTCTTAGAGTTGCTTGCTGGTGTGTCCAAGATGATGAGGCTCATAGACCATCAATGGGTCAAGTGGTGCAGATTCTCGAGGGGGTTTTGAATGTGAACTTGCCCTCAGTTCCAAGATCTCTCCAACTCTTTGGAGATAGCCAGCACATAGTTTTCTTCACCGAGTCATCTGCAAGCCAGAGCTCGCATGCACCAAGCAATACTACATCAACAGCTTCATCGATCTTAGACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

B_lectin PAN_2 Pkinase S_locus_glycop

Protein Analysis

822

Amino Acids

91.45

Weight (kDa)

6.84

Isoelectric Point (pI)

38.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	83 - 187	1.6e-33	D-mannose binding lectin
S_locus_glycop	PF00954	220 - 329	7.2e-29	S-locus glycoprotein domain
PAN_2	PF08276	360 - 416	3.1e-14	PAN-like domain
PK_Tyr_Ser-Thr	PF07714	502 - 770	2.8e-40	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	503 - 769	1.7e-46	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0006169)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G19130
fragaria_vesca	FvH4_6g44310
malus_domestica	MD09G1098900.v1.1 MD17G1087200.v1.1
prunus_persica	Prupe.3G227300_v2.0.a1 Prupe.3G227500_v2.0.a1
pyrus_communis	pycom09g02260 pycom17g08440
rosa_chinensis	RchiOBHm_Chr2g0161171 RchiOBHm_Chr2g0161191
rosa_laevigata	RLG00000021264 RLG00000021266 RLG00000029382
rosa_multiflora	Rmu_sc0006420.1_g000040 Rmu_sc0006420.1_g000045
rosa_roxburghii	Rroxscaffold_2G00089040 Rroxscaffold_2G00089060
rosa_rugosa	Rorug02G0486800 Rorug02G0486900
rosa_samantha	Rh2AG553500 Rh2BG567200 Rh2CG537300 Rh2CG537500 Rh2DG576500 Rh2DG576600
rosa_wichuraiana	Rw2G045780 Rw2G045800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	947
Acc16I	TGCGCA	1 cut(s)	1155
Acc36I	ACCTGC	1 cut(s)	1000
AccB1I	GGYRCC	1 cut(s)	1694
AccB7I	CCANNNNNTGG	1 cut(s)	255
AccBSI	CCGCTC	1 cut(s)	1185
AciI	CCGC	1 cut(s)	1183
AclWI	GGATC	5 cut(s)	566, 579, 1316, 1329, 1741
AcoI	YGGCCR	2 cut(s)	12, 261
AcsI	RAATTY	6 cut(s)	38, 161, 562, 1003, 1048, 1952
AcuI	CTGAAG	4 cut(s)	282, 721, 1710, 2127
AcyI	GRCGYC	1 cut(s)	944
AfaI	GTAC	4 cut(s)	221, 237, 1292, 1810
AfiI	CCNNNNNNNGG	8 cut(s)	255, 357, 581, 918, 1074, 2137, 2201, 2291
AflII	CTTAAG	2 cut(s)	61, 1174
AflIII	ACRYGT	2 cut(s)	397, 1654
AgeI	ACCGGT	1 cut(s)	1086
AhlI	ACTAGT	1 cut(s)	662
AjnI	CCWGG	4 cut(s)	184, 845, 1929, 1987
AjuI	GAANNNNNNNTTGG	2 cut(s)	495, 527
AleI	CACNNNNGTG	1 cut(s)	2005
Alw21I	GWGCWC	1 cut(s)	2420
Alw26I	GTCTC	5 cut(s)	2, 126, 286, 885, 935
AlwI	GGATC	5 cut(s)	566, 579, 1316, 1329, 1741
AlwNI	CAGNNNCTG	1 cut(s)	284
Ama87I	CYCGRG	3 cut(s)	1803, 2136, 2312
AoxI	GGCC	4 cut(s)	12, 261, 1393, 1927
ApeKI	GCWGC	4 cut(s)	274, 1026, 1304, 2141
ApoI	RAATTY	6 cut(s)	38, 161, 562, 1003, 1048, 1952
AsiGI	ACCGGT	1 cut(s)	1086
Asp700I	GAANNNNTTC	1 cut(s)	1616
AspLEI	GCGC	1 cut(s)	1156
AspS9I	GGNCC	2 cut(s)	360, 2191
AsuC2I	CCSGG	1 cut(s)	582
AsuHPI	GGTGA	6 cut(s)	143, 763, 1286, 1739, 2225, 2386
AsuII	TTCGAA	1 cut(s)	2070
AsuNHI	GCTAGC	1 cut(s)	46
AvaI	CYCGRG	3 cut(s)	1803, 2136, 2312
AvaII	GGWCC	2 cut(s)	360, 2191
BaeGI	GKGCMC	1 cut(s)	1553
BaeI	ACNNNNGTAYC	2 cut(s)	219, 252
BalI	TGGCCA	1 cut(s)	263
BamHI	GGATCC	2 cut(s)	571, 1321
BanI	GGYRCC	1 cut(s)	1694
BanII	GRGCYC	1 cut(s)	2420
BauI	CACGAG	2 cut(s)	153, 2155
BbsI	GAAGAC	3 cut(s)	1534, 2092, 2115
Bbv12I	GWGCWC	1 cut(s)	2420
BbvI	GCAGC	4 cut(s)	286, 1013, 1316, 2128
BccI	CCATC	6 cut(s)	113, 311, 858, 1271, 2110, 2293
BceAI	ACGGC	2 cut(s)	27, 2128
BcgI	CGANNNNNNTGC	2 cut(s)	2387, 2421
BciT130I	CCWGG	4 cut(s)	186, 847, 1931, 1989
BclI	TGATCA	1 cut(s)	2151
BcnI	CCSGG	1 cut(s)	582
BcoDI	GTCTC	5 cut(s)	2, 126, 286, 885, 935
BcuI	ACTAGT	1 cut(s)	662
BfaI	CTAG	7 cut(s)	47, 507, 596, 663, 792, 1104, 1892
BfmI	CTRYAG	1 cut(s)	1134
BfrI	CTTAAG	2 cut(s)	61, 1174
BfuAI	ACCTGC	1 cut(s)	1000
BglI	GCCNNNNNGGC	1 cut(s)	463
BglII	AGATCT	3 cut(s)	1237, 1480, 2351
BisI	GCNGC	4 cut(s)	275, 1027, 1305, 2142
BlpI	GCTNAGC	2 cut(s)	1366, 2180
BlsI	GCNGC	4 cut(s)	276, 1028, 1306, 2143
Bme1390I	CCNGG	5 cut(s)	186, 582, 847, 1931, 1989
Bme18I	GGWCC	2 cut(s)	360, 2191
BmeT110I	CYCGRG	3 cut(s)	1803, 2136, 2312
BmgT120I	GGNCC	2 cut(s)	360, 2191
BmiI	GGNNCC	5 cut(s)	573, 705, 1323, 1696, 2193
BmrFI	CCNGG	5 cut(s)	186, 582, 847, 1931, 1989
BmrI	ACTGGG	1 cut(s)	179
BmsI	GCATC	6 cut(s)	667, 702, 724, 804, 1732, 2052
BmtI	GCTAGC	1 cut(s)	50
BmuI	ACTGGG	1 cut(s)	179
BpiI	GAAGAC	3 cut(s)	1534, 2092, 2115
Bpu1102I	GCTNAGC	2 cut(s)	1366, 2180
Bpu14I	TTCGAA	1 cut(s)	2070
BpuEI	CTTGAG	1 cut(s)	59
BpuMI	CCSGG	1 cut(s)	582
Bsa29I	ATCGAT	1 cut(s)	2456
BsaHI	GRCGYC	1 cut(s)	944
BsaI	GGTCTC	1 cut(s)	885
BsaJI	CCNNGG	3 cut(s)	15, 887, 1603
BsaWI	WCCGGW	1 cut(s)	1086
BsaXI	ACNNNNNCTCC	4 cut(s)	2010, 2040, 2197, 2227
Bsc4I	CCNNNNNNNGG	8 cut(s)	255, 357, 581, 918, 1074, 2137, 2201, 2291
Bse118I	RCCGGY	1 cut(s)	1086
Bse1I	ACTGG	3 cut(s)	118, 174, 278
Bse3DI	GCAATG	3 cut(s)	943, 1163, 1219
BseBI	CCWGG	4 cut(s)	186, 847, 1931, 1989
BseCI	ATCGAT	1 cut(s)	2456
BseDI	CCNNGG	3 cut(s)	15, 887, 1603
BseGI	GGATG	5 cut(s)	682, 823, 869, 1223, 1747
BseLI	CCNNNNNNNGG	8 cut(s)	255, 357, 581, 918, 1074, 2137, 2201, 2291
BseMI	GCAATG	3 cut(s)	943, 1163, 1219
BseMII	CTCAG	8 cut(s)	327, 699, 896, 1309, 1380, 2088, 2171, 2355
BseNI	ACTGG	3 cut(s)	118, 174, 278
BseRI	GAGGAG	1 cut(s)	1539
BseSI	GKGCMC	1 cut(s)	1553
BseXI	GCAGC	4 cut(s)	286, 1013, 1316, 2128
BseYI	CCCAGC	2 cut(s)	437, 1142
BsgI	GTGCAG	2 cut(s)	561, 2324
BshFI	GGCC	4 cut(s)	14, 263, 1395, 1929
BshNI	GGYRCC	1 cut(s)	1694
BshTI	ACCGGT	1 cut(s)	1086
BshVI	ATCGAT	1 cut(s)	2456
BsiHKAI	GWGCWC	1 cut(s)	2420
BsiHKCI	CYCGRG	3 cut(s)	1803, 2136, 2312
BsiSI	CCGG	2 cut(s)	581, 1087
BslFI	GGGAC	3 cut(s)	64, 1657, 1893
BslI	CCNNNNNNNGG	8 cut(s)	255, 357, 581, 918, 1074, 2137, 2201, 2291
BsmAI	GTCTC	5 cut(s)	2, 126, 286, 885, 935
BsmBI	CGTCTC	2 cut(s)	2, 935
BsmFI	GGGAC	3 cut(s)	64, 1657, 1893
BsnI	GGCC	4 cut(s)	14, 263, 1395, 1929
Bso31I	GGTCTC	1 cut(s)	885
BsoBI	CYCGRG	3 cut(s)	1803, 2136, 2312
Bsp119I	TTCGAA	1 cut(s)	2070
Bsp1286I	GDGCHC	2 cut(s)	1553, 2420
Bsp1720I	GCTNAGC	2 cut(s)	1366, 2180
Bsp19I	CCATGG	1 cut(s)	15
BspACI	CCGC	1 cut(s)	1183
BspANI	GGCC	4 cut(s)	14, 263, 1395, 1929
BspCNI	CTCAG	8 cut(s)	326, 700, 895, 1308, 1379, 2089, 2172, 2354
BspDI	ATCGAT	1 cut(s)	2456
BspHI	TCATGA	2 cut(s)	1834, 2229
BspLI	GGNNCC	5 cut(s)	573, 705, 1323, 1696, 2193
BspMI	ACCTGC	1 cut(s)	1000
BspOI	GCTAGC	1 cut(s)	50
BspPI	GGATC	5 cut(s)	566, 579, 1316, 1329, 1741
BspQI	GCTCTTC	1 cut(s)	2072
BspT104I	TTCGAA	1 cut(s)	2070
BspT107I	GGYRCC	1 cut(s)	1694
BspTI	CTTAAG	2 cut(s)	61, 1174
BspTNI	GGTCTC	1 cut(s)	885
BsrBI	CCGCTC	1 cut(s)	1185
BsrDI	GCAATG	3 cut(s)	943, 1163, 1219
BsrFI	RCCGGY	1 cut(s)	1086
BsrI	ACTGG	3 cut(s)	118, 174, 278
BssAI	RCCGGY	1 cut(s)	1086
BssECI	CCNNGG	3 cut(s)	15, 887, 1603
BssNI	GRCGYC	1 cut(s)	944
BssSI	CACGAG	2 cut(s)	153, 2155
BssT1I	CCWWGG	2 cut(s)	15, 1603
Bst2BI	CACGAG	2 cut(s)	153, 2155
Bst2UI	CCWGG	4 cut(s)	186, 847, 1931, 1989
Bst4CI	ACNGT	6 cut(s)	235, 526, 636, 1042, 1124, 1274
Bst6I	CTCTTC	4 cut(s)	394, 1942, 2072, 2217
BstACI	GRCGYC	1 cut(s)	944
BstAFI	CTTAAG	2 cut(s)	61, 1174
BstBI	TTCGAA	1 cut(s)	2070
BstC8I	GCNNGC	7 cut(s)	48, 1985, 2251, 2378, 2410, 2420, 2424
BstDSI	CCRYGG	1 cut(s)	15
BstEII	GGTNACC	1 cut(s)	131
BstF5I	GGATG	5 cut(s)	682, 823, 869, 1223, 1747
BstHHI	GCGC	1 cut(s)	1156
BstMAI	GTCTC	5 cut(s)	2, 126, 286, 885, 935
BstMWI	GCNNNNNNNGC	5 cut(s)	52, 463, 677, 1989, 2030
BstNI	CCWGG	4 cut(s)	186, 847, 1931, 1989
BstNSI	RCATGY	6 cut(s)	217, 401, 1111, 1658, 1726, 2426
BstPI	GGTNACC	1 cut(s)	131
BstSCI	CCNGG	5 cut(s)	184, 580, 845, 1929, 1987
BstSFI	CTRYAG	1 cut(s)	1134
BstSLI	GKGCMC	1 cut(s)	1553
BstV1I	GCAGC	4 cut(s)	286, 1013, 1316, 2128
BstV2I	GAAGAC	3 cut(s)	1534, 2092, 2115
BstX2I	RGATCY	6 cut(s)	571, 1237, 1321, 1480, 1733, 2351
BstXI	CCANNNNNNTGG	1 cut(s)	2366
BstYI	RGATCY	6 cut(s)	571, 1237, 1321, 1480, 1733, 2351
Bsu15I	ATCGAT	1 cut(s)	2456
BsuRI	GGCC	4 cut(s)	14, 263, 1395, 1929
BsuTUI	ATCGAT	1 cut(s)	2456
BtgI	CCRYGG	1 cut(s)	15
BtgZI	GCGATG	2 cut(s)	1782, 2187
BtsCI	GGATG	5 cut(s)	682, 823, 869, 1223, 1747
BtsI	GCAGTG	2 cut(s)	1191, 1584
BtsIMutI	CAGTG	6 cut(s)	522, 632, 1129, 1191, 1452, 1584
BveI	ACCTGC	1 cut(s)	1000
Cac8I	GCNNGC	7 cut(s)	48, 1985, 2251, 2378, 2410, 2420, 2424
CaiI	CAGNNNCTG	1 cut(s)	284
CciI	TCATGA	2 cut(s)	1834, 2229
CfoI	GCGC	1 cut(s)	1156
Cfr10I	RCCGGY	1 cut(s)	1086
Cfr13I	GGNCC	2 cut(s)	360, 2191
ClaI	ATCGAT	1 cut(s)	2456
Csp6I	GTAC	4 cut(s)	220, 236, 1291, 1809
CspAI	ACCGGT	1 cut(s)	1086
CviQI	GTAC	4 cut(s)	220, 236, 1291, 1809
EaeI	YGGCCR	2 cut(s)	12, 261
Eam1104I	CTCTTC	4 cut(s)	394, 1942, 2072, 2217
EarI	CTCTTC	4 cut(s)	394, 1942, 2072, 2217
Ecl136II	GAGCTC	1 cut(s)	2418
Eco130I	CCWWGG	2 cut(s)	15, 1603
Eco147I	AGGCCT	1 cut(s)	1395
Eco24I	GRGCYC	1 cut(s)	2420
Eco31I	GGTCTC	1 cut(s)	885
Eco47I	GGWCC	2 cut(s)	360, 2191
Eco53kI	GAGCTC	1 cut(s)	2418
Eco57I	CTGAAG	4 cut(s)	282, 721, 1710, 2127
Eco88I	CYCGRG	3 cut(s)	1803, 2136, 2312
Eco91I	GGTNACC	1 cut(s)	131
EcoICRI	GAGCTC	1 cut(s)	2418
EcoO65I	GGTNACC	1 cut(s)	131
EcoRI	GAATTC	2 cut(s)	1003, 1952
EcoRII	CCWGG	4 cut(s)	184, 845, 1929, 1987
EcoT14I	CCWWGG	2 cut(s)	15, 1603
EcoT22I	ATGCAT	1 cut(s)	717
EcoT38I	GRGCYC	1 cut(s)	2420
ErhI	CCWWGG	2 cut(s)	15, 1603
Esp3I	CGTCTC	2 cut(s)	2, 935
FaqI	GGGAC	3 cut(s)	64, 1657, 1893
FbaI	TGATCA	1 cut(s)	2151
Fnu4HI	GCNGC	4 cut(s)	275, 1027, 1305, 2142
FokI	GGATG	5 cut(s)	689, 830, 876, 1230, 1754
FriOI	GRGCYC	1 cut(s)	2420
Fsp4HI	GCNGC	4 cut(s)	275, 1027, 1305, 2142
FspBI	CTAG	7 cut(s)	47, 507, 596, 663, 792, 1104, 1892
FspI	TGCGCA	1 cut(s)	1155
GlaI	GCGC	1 cut(s)	1155
GluI	GCNGC	4 cut(s)	275, 1027, 1305, 2142
GsaI	CCCAGC	2 cut(s)	441, 1146
HaeIII	GGCC	4 cut(s)	14, 263, 1395, 1929
HapII	CCGG	2 cut(s)	581, 1087
HhaI	GCGC	1 cut(s)	1156
Hin1I	GRCGYC	1 cut(s)	944
Hin6I	GCGC	1 cut(s)	1154
HinP1I	GCGC	1 cut(s)	1154
HindIII	AAGCTT	1 cut(s)	1937
HpaII	CCGG	2 cut(s)	581, 1087
HphI	GGTGA	6 cut(s)	143, 763, 1286, 1739, 2225, 2386
Hpy166II	GTNNAC	3 cut(s)	363, 2047, 2332
Hpy8I	GTNNAC	3 cut(s)	363, 2047, 2332
HpyAV	CCTTC	4 cut(s)	964, 985, 1685, 2162
HpyCH4III	ACNGT	6 cut(s)	235, 526, 636, 1042, 1124, 1274
HpyCH4IV	ACGT	1 cut(s)	944
HpyF10VI	GCNNNNNNNGC	5 cut(s)	52, 463, 677, 1989, 2030
HpySE526I	ACGT	1 cut(s)	944
Hsp92I	GRCGYC	1 cut(s)	944
HspAI	GCGC	1 cut(s)	1154
Ksp22I	TGATCA	1 cut(s)	2151
LguI	GCTCTTC	1 cut(s)	2072
LmnI	GCTCC	3 cut(s)	95, 271, 668
Lsp1109I	GCAGC	4 cut(s)	286, 1013, 1316, 2128
LweI	GCATC	6 cut(s)	667, 702, 724, 804, 1732, 2052
MaeI	CTAG	7 cut(s)	47, 507, 596, 663, 792, 1104, 1892
MaeII	ACGT	1 cut(s)	944
MaeIII	GTNAC	7 cut(s)	131, 526, 1178, 1492, 1865, 2026, 2159
MbiI	CCGCTC	1 cut(s)	1185
MfeI	CAATTG	1 cut(s)	1638
MflI	RGATCY	6 cut(s)	571, 1237, 1321, 1480, 1733, 2351
MhlI	GDGCHC	2 cut(s)	1553, 2420
MlsI	TGGCCA	1 cut(s)	263
MluNI	TGGCCA	1 cut(s)	263
MlyI	GAGTC	6 cut(s)	29, 350, 599, 694, 1971, 2408
MmeI	TCCRAC	3 cut(s)	1675, 1924, 2383
Mox20I	TGGCCA	1 cut(s)	263
Mph1103I	ATGCAT	1 cut(s)	717
MroXI	GAANNNNTTC	1 cut(s)	1616
MscI	TGGCCA	1 cut(s)	263
MseI	TTAA	6 cut(s)	62, 336, 747, 834, 1175, 1317
MslI	CAYNNNNRTG	3 cut(s)	212, 1577, 2005
Msp20I	TGGCCA	1 cut(s)	263
MspA1I	CMGCKG	1 cut(s)	1370
MspCI	CTTAAG	2 cut(s)	61, 1174
MspI	CCGG	2 cut(s)	581, 1087
MspR9I	CCNGG	5 cut(s)	186, 582, 847, 1931, 1989
MunI	CAATTG	1 cut(s)	1638
MvaI	CCWGG	4 cut(s)	186, 847, 1931, 1989
MwoI	GCNNNNNNNGC	5 cut(s)	52, 463, 677, 1989, 2030
NciI	CCSGG	1 cut(s)	582
NcoI	CCATGG	1 cut(s)	15
NheI	GCTAGC	1 cut(s)	46
NlaIV	GGNNCC	5 cut(s)	573, 705, 1323, 1696, 2193
NmeAIII	GCCGAG	2 cut(s)	912, 1985
NmuCI	GTSAC	3 cut(s)	131, 1865, 2026
NsbI	TGCGCA	1 cut(s)	1155
NsiI	ATGCAT	1 cut(s)	717
NspI	RCATGY	6 cut(s)	217, 401, 1111, 1658, 1726, 2426
NspV	TTCGAA	1 cut(s)	2070
OliI	CACNNNNGTG	1 cut(s)	2005
PaeI	GCATGC	1 cut(s)	2426
PaeR7I	CTCGAG	1 cut(s)	2312
PagI	TCATGA	2 cut(s)	1834, 2229
PceI	AGGCCT	1 cut(s)	1395
PciI	ACATGT	2 cut(s)	397, 1654
PciSI	GCTCTTC	1 cut(s)	2072
PcsI	WCGNNNNNNNCGW	1 cut(s)	2169
PdmI	GAANNNNTTC	1 cut(s)	1616
PfeI	GAWTC	8 cut(s)	309, 788, 807, 1163, 1246, 1562, 2124, 2308
PflMI	CCANNNNNTGG	1 cut(s)	255
PinAI	ACCGGT	1 cut(s)	1086
PkrI	GCNGC	4 cut(s)	276, 1028, 1306, 2143
PleI	GAGTC	6 cut(s)	28, 349, 599, 693, 1970, 2407
PpsI	GAGTC	6 cut(s)	28, 349, 599, 693, 1970, 2407
PscI	ACATGT	2 cut(s)	397, 1654
Psp124BI	GAGCTC	1 cut(s)	2420
Psp6I	CCWGG	4 cut(s)	184, 845, 1929, 1987
PspEI	GGTNACC	1 cut(s)	131
PspFI	CCCAGC	2 cut(s)	437, 1142
PspGI	CCWGG	4 cut(s)	184, 845, 1929, 1987
PspN4I	GGNNCC	5 cut(s)	573, 705, 1323, 1696, 2193
PspPI	GGNCC	2 cut(s)	360, 2191
PstNI	CAGNNNCTG	1 cut(s)	284
PsuI	RGATCY	6 cut(s)	571, 1237, 1321, 1480, 1733, 2351
PvuII	CAGCTG	1 cut(s)	1370
RsaI	GTAC	4 cut(s)	221, 237, 1292, 1810
RsaNI	GTAC	4 cut(s)	220, 236, 1291, 1809
RseI	CAYNNNNRTG	3 cut(s)	212, 1577, 2005
SacI	GAGCTC	1 cut(s)	2420
SapI	GCTCTTC	1 cut(s)	2072
SaqAI	TTAA	6 cut(s)	62, 336, 747, 834, 1175, 1317
SatI	GCNGC	4 cut(s)	275, 1027, 1305, 2142
Sau96I	GGNCC	2 cut(s)	360, 2191
SchI	GAGTC	6 cut(s)	29, 350, 599, 694, 1971, 2408
ScrFI	CCNGG	5 cut(s)	186, 582, 847, 1931, 1989
SduI	GDGCHC	2 cut(s)	1553, 2420
SfaNI	GCATC	6 cut(s)	667, 702, 724, 804, 1732, 2052
SfcI	CTRYAG	1 cut(s)	1134
Sfr274I	CTCGAG	1 cut(s)	2312
SfuI	TTCGAA	1 cut(s)	2070
SinI	GGWCC	2 cut(s)	360, 2191
SlaI	CTCGAG	1 cut(s)	2312
SmiMI	CAYNNNNRTG	3 cut(s)	212, 1577, 2005
SmlI	CTYRAG	4 cut(s)	61, 74, 1174, 2312
SmoI	CTYRAG	4 cut(s)	61, 74, 1174, 2312
SpeI	ACTAGT	1 cut(s)	662
SphI	GCATGC	1 cut(s)	2426
SseBI	AGGCCT	1 cut(s)	1395
SsiI	CCGC	1 cut(s)	1183
SspMI	CTAG	7 cut(s)	47, 507, 596, 663, 792, 1104, 1892
SstI	GAGCTC	1 cut(s)	2420
StuI	AGGCCT	1 cut(s)	1395
StyD4I	CCNGG	5 cut(s)	184, 580, 845, 1929, 1987
StyI	CCWWGG	2 cut(s)	15, 1603
TaaI	ACNGT	6 cut(s)	235, 526, 636, 1042, 1124, 1274
TaiI	ACGT	1 cut(s)	947
TaqI	TCGA	5 cut(s)	608, 805, 2070, 2313, 2456
TfiI	GAWTC	8 cut(s)	309, 788, 807, 1163, 1246, 1562, 2124, 2308
Tru1I	TTAA	6 cut(s)	62, 336, 747, 834, 1175, 1317
Tru9I	TTAA	6 cut(s)	62, 336, 747, 834, 1175, 1317
TscAI	CASTG	6 cut(s)	529, 639, 1129, 1198, 1452, 1584
TseFI	GTSAC	3 cut(s)	131, 1865, 2026
TseI	GCWGC	4 cut(s)	274, 1026, 1304, 2141
Tsp45I	GTSAC	3 cut(s)	131, 1865, 2026
TspDTI	ATGAA	7 cut(s)	438, 543, 799, 1446, 1554, 1823, 2442
TspGWI	ACGGA	2 cut(s)	1051, 2070
TspRI	CASTG	6 cut(s)	529, 639, 1129, 1198, 1452, 1584
Van91I	CCANNNNNTGG	1 cut(s)	255
Vha464I	CTTAAG	2 cut(s)	61, 1174
VpaK11BI	GGWCC	2 cut(s)	360, 2191
XapI	RAATTY	6 cut(s)	38, 161, 562, 1003, 1048, 1952
XceI	RCATGY	6 cut(s)	217, 401, 1111, 1658, 1726, 2426
XcmI	CCANNNNNNNNNTGG	5 cut(s)	89, 125, 256, 489, 1705
XhoI	CTCGAG	1 cut(s)	2312
XmnI	GAANNNNTTC	1 cut(s)	1616
XspI	CTAG	7 cut(s)	47, 507, 596, 663, 792, 1104, 1892
ZraI	GACGTC	1 cut(s)	945
Zsp2I	ATGCAT	1 cut(s)	717

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.